Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05722.t1 | NP_001235659.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 3.30E-62 | 247.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05722.t1 | P0DI19 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.1e-64 | 246.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05722.t1 | M5W1X1 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.4e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene05722 | MS.gene53861 | PPI |
| MS.gene05722 | MS.gene008298 | PPI |
| MS.gene05722 | MS.gene008697 | PPI |
| MS.gene002214 | MS.gene05722 | PPI |
| MS.gene05722 | MS.gene04991 | PPI |
| MS.gene05722 | MS.gene029826 | PPI |
| MS.gene048485 | MS.gene05722 | PPI |
| MS.gene05722 | MS.gene75012 | PPI |
| MS.gene05722 | MS.gene01187 | PPI |
| MS.gene05722 | MS.gene53859 | PPI |
| MS.gene05722 | MS.gene071905 | PPI |
| MS.gene05722 | MS.gene24607 | PPI |
| MS.gene05722 | MS.gene048687 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05722.t1 | MTR_3g464510 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.57e-77 | 223 |
| MS.gene05722.t1 | MTR_4g022900 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.44e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05722.t1 | AT2G30000 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene05722.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCTAAATTGACAATTT+TGG | 0.090409 | 3.1:+48429030 | None:intergenic |
| TGCCCCAAAATTGTCAATTT+AGG | 0.226054 | 3.1:-48429034 | MS.gene05722:CDS |
| GTGCAACTATGGATCATTTC+AGG | 0.247422 | 3.1:-48429143 | MS.gene05722:CDS |
| TTCATAGAATAAATCAGTTT+TGG | 0.292411 | 3.1:+48429006 | None:intergenic |
| TTCTATGAACGCAAGAAGTA+TGG | 0.313430 | 3.1:-48428992 | MS.gene05722:CDS |
| GCCCCAAAATTGTCAATTTA+GGG | 0.323285 | 3.1:-48429033 | MS.gene05722:CDS |
| GCACACAGCAGGAGAAAGAT+AGG | 0.403394 | 3.1:-48429063 | MS.gene05722:CDS |
| TGCGTCCTTGTACACTTGTA+AGG | 0.414410 | 3.1:-48429177 | MS.gene05722:CDS |
| GTCTGTGATGAGTGCAACTA+TGG | 0.415210 | 3.1:-48429154 | MS.gene05722:CDS |
| AAGCAGCCAGGAATTGCTAT+TGG | 0.443682 | 3.1:-48429250 | MS.gene05722:CDS |
| TAGCAATTCCTGGCTGCTTA+CGG | 0.455501 | 3.1:+48429254 | None:intergenic |
| AATCGTCCAATAGCAATTCC+TGG | 0.467819 | 3.1:+48429244 | None:intergenic |
| TTACGGCACATGATCAAATC+AGG | 0.468198 | 3.1:+48429271 | None:intergenic |
| ATCATGTGCCGTAAGCAGCC+AGG | 0.536811 | 3.1:-48429262 | MS.gene05722:CDS |
| TGCAACTATGGATCATTTCA+GGG | 0.537300 | 3.1:-48429142 | MS.gene05722:CDS |
| CAGACCCTTACAAGTGTACA+AGG | 0.539307 | 3.1:+48429172 | None:intergenic |
| CTGCAAGGAATGCACACAGC+AGG | 0.557725 | 3.1:-48429074 | MS.gene05722:CDS |
| GATCAAATCAGGATGATGCT+TGG | 0.567091 | 3.1:+48429282 | None:intergenic |
| TGTGTCATATGTGGAGGAGT+AGG | 0.572461 | 3.1:-48429115 | MS.gene05722:CDS |
| CAGGGTCGATGTGTCATATG+TGG | 0.573549 | 3.1:-48429124 | MS.gene05722:CDS |
| TTCCTTGCAGTAGTAAGCAT+CGG | 0.596378 | 3.1:+48429087 | None:intergenic |
| ATCCGATGCTTACTACTGCA+AGG | 0.604803 | 3.1:-48429089 | MS.gene05722:CDS |
| GCGTCCTTGTACACTTGTAA+GGG | 0.606045 | 3.1:-48429176 | MS.gene05722:CDS |
| CACACAGCAGGAGAAAGATA+GGG | 0.609822 | 3.1:-48429062 | MS.gene05722:CDS |
| AAGTGTACAAGGACGCACAT+AGG | 0.637033 | 3.1:+48429183 | None:intergenic |
| GGTCGATGTGTCATATGTGG+AGG | 0.641454 | 3.1:-48429121 | MS.gene05722:CDS |
| CGATTATGCGAGAAATGTGA+CGG | 0.661791 | 3.1:-48429226 | MS.gene05722:CDS |
| CAGCAGGAGAAAGATAGGGA+TGG | 0.676444 | 3.1:-48429058 | MS.gene05722:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATAGAATAAATCAGTTT+TGG | + | chr3.1:48429259-48429278 | None:intergenic | 20.0% |
| ! | ACTCCCTAAATTGACAATTT+TGG | + | chr3.1:48429235-48429254 | None:intergenic | 30.0% |
| ! | CTCCCTAAATTGACAATTTT+GGG | + | chr3.1:48429234-48429253 | None:intergenic | 30.0% |
| ! | TCCCTAAATTGACAATTTTG+GGG | + | chr3.1:48429233-48429252 | None:intergenic | 30.0% |
| GCCCCAAAATTGTCAATTTA+GGG | - | chr3.1:48429229-48429248 | MS.gene05722:CDS | 35.0% | |
| TGCAACTATGGATCATTTCA+GGG | - | chr3.1:48429120-48429139 | MS.gene05722:CDS | 35.0% | |
| TGCCCCAAAATTGTCAATTT+AGG | - | chr3.1:48429228-48429247 | MS.gene05722:CDS | 35.0% | |
| TTCTATGAACGCAAGAAGTA+TGG | - | chr3.1:48429270-48429289 | MS.gene05722:CDS | 35.0% | |
| AATCGTCCAATAGCAATTCC+TGG | + | chr3.1:48429021-48429040 | None:intergenic | 40.0% | |
| CGATTATGCGAGAAATGTGA+CGG | - | chr3.1:48429036-48429055 | MS.gene05722:CDS | 40.0% | |
| GATCAAATCAGGATGATGCT+TGG | + | chr3.1:48428983-48429002 | None:intergenic | 40.0% | |
| GTGCAACTATGGATCATTTC+AGG | - | chr3.1:48429119-48429138 | MS.gene05722:CDS | 40.0% | |
| TTACGGCACATGATCAAATC+AGG | + | chr3.1:48428994-48429013 | None:intergenic | 40.0% | |
| TTCCTTGCAGTAGTAAGCAT+CGG | + | chr3.1:48429178-48429197 | None:intergenic | 40.0% | |
| AAGCAGCCAGGAATTGCTAT+TGG | - | chr3.1:48429012-48429031 | MS.gene05722:CDS | 45.0% | |
| AAGTGTACAAGGACGCACAT+AGG | + | chr3.1:48429082-48429101 | None:intergenic | 45.0% | |
| ATCCGATGCTTACTACTGCA+AGG | - | chr3.1:48429173-48429192 | MS.gene05722:CDS | 45.0% | |
| CACACAGCAGGAGAAAGATA+GGG | - | chr3.1:48429200-48429219 | MS.gene05722:CDS | 45.0% | |
| CAGACCCTTACAAGTGTACA+AGG | + | chr3.1:48429093-48429112 | None:intergenic | 45.0% | |
| GCGTCCTTGTACACTTGTAA+GGG | - | chr3.1:48429086-48429105 | MS.gene05722:CDS | 45.0% | |
| GTCTGTGATGAGTGCAACTA+TGG | - | chr3.1:48429108-48429127 | MS.gene05722:CDS | 45.0% | |
| TAGCAATTCCTGGCTGCTTA+CGG | + | chr3.1:48429011-48429030 | None:intergenic | 45.0% | |
| TGCGTCCTTGTACACTTGTA+AGG | - | chr3.1:48429085-48429104 | MS.gene05722:CDS | 45.0% | |
| TGTGTCATATGTGGAGGAGT+AGG | - | chr3.1:48429147-48429166 | MS.gene05722:CDS | 45.0% | |
| CAGCAGGAGAAAGATAGGGA+TGG | - | chr3.1:48429204-48429223 | MS.gene05722:CDS | 50.0% | |
| CAGGGTCGATGTGTCATATG+TGG | - | chr3.1:48429138-48429157 | MS.gene05722:CDS | 50.0% | |
| GCACACAGCAGGAGAAAGAT+AGG | - | chr3.1:48429199-48429218 | MS.gene05722:CDS | 50.0% | |
| GGTCGATGTGTCATATGTGG+AGG | - | chr3.1:48429141-48429160 | MS.gene05722:CDS | 50.0% | |
| ATCATGTGCCGTAAGCAGCC+AGG | - | chr3.1:48429000-48429019 | MS.gene05722:CDS | 55.0% | |
| CTGCAAGGAATGCACACAGC+AGG | - | chr3.1:48429188-48429207 | MS.gene05722:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 48428976 | 48429308 | 48428976 | ID=MS.gene05722 |
| chr3.1 | mRNA | 48428976 | 48429308 | 48428976 | ID=MS.gene05722.t1;Parent=MS.gene05722 |
| chr3.1 | exon | 48428976 | 48429308 | 48428976 | ID=MS.gene05722.t1.exon1;Parent=MS.gene05722.t1 |
| chr3.1 | CDS | 48428976 | 48429308 | 48428976 | ID=cds.MS.gene05722.t1;Parent=MS.gene05722.t1 |
| Gene Sequence |
| Protein sequence |
