Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048485.t1 | NP_001235659.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 3.30E-62 | 247.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048485.t1 | P0DI19 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.1e-64 | 246.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048485.t1 | M5W1X1 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.4e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene048485 | MS.gene008298 | PPI |
| MS.gene048485 | MS.gene008697 | PPI |
| MS.gene048485 | MS.gene04991 | PPI |
| MS.gene048485 | MS.gene029826 | PPI |
| MS.gene048485 | MS.gene05722 | PPI |
| MS.gene048485 | MS.gene75012 | PPI |
| MS.gene048485 | MS.gene01187 | PPI |
| MS.gene048485 | MS.gene002214 | PPI |
| MS.gene048485 | MS.gene53859 | PPI |
| MS.gene048485 | MS.gene24607 | PPI |
| MS.gene048485 | MS.gene048687 | PPI |
| MS.gene048485 | MS.gene53861 | PPI |
| MS.gene048485 | MS.gene071905 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048485.t1 | MTR_3g464510 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.57e-77 | 223 |
| MS.gene048485.t1 | MTR_4g022900 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.44e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048485.t1 | AT2G30000 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene048485.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCTAAATTGACAATTT+TGG | 0.090409 | 3.3:+52004102 | None:intergenic |
| TGCCCCAAAATTGTCAATTT+AGG | 0.226054 | 3.3:-52004106 | MS.gene048485:CDS |
| TTCTATGAACGCAAGAAGTA+TGG | 0.233785 | 3.3:-52004064 | MS.gene048485:CDS |
| ATGCAACTATGGATCATTTC+AGG | 0.249118 | 3.3:-52004215 | MS.gene048485:CDS |
| TTCATAGAATAAATCAGTTT+TGG | 0.292411 | 3.3:+52004078 | None:intergenic |
| GCCCCAAAATTGTCAATTTA+GGG | 0.323285 | 3.3:-52004105 | MS.gene048485:CDS |
| GCACACAGCAGGAGAAAGAT+AGG | 0.403394 | 3.3:-52004135 | MS.gene048485:CDS |
| TGCGTCCTTGTACACTTGTA+AGG | 0.414410 | 3.3:-52004249 | MS.gene048485:CDS |
| GTCTGTGATGAATGCAACTA+TGG | 0.439271 | 3.3:-52004226 | MS.gene048485:CDS |
| AAGCAGCCAGGAATTGCTAT+TGG | 0.443682 | 3.3:-52004322 | MS.gene048485:CDS |
| TAGCAATTCCTGGCTGCTTA+CGG | 0.455501 | 3.3:+52004326 | None:intergenic |
| AATCGTCCAATAGCAATTCC+TGG | 0.467819 | 3.3:+52004316 | None:intergenic |
| TTACGGCACATGATCAAATC+AGG | 0.468198 | 3.3:+52004343 | None:intergenic |
| CAGGGTCGATGCGTTATATG+TGG | 0.508420 | 3.3:-52004196 | MS.gene048485:CDS |
| TGCAACTATGGATCATTTCA+GGG | 0.533649 | 3.3:-52004214 | MS.gene048485:CDS |
| ATCATGTGCCGTAAGCAGCC+AGG | 0.536811 | 3.3:-52004334 | MS.gene048485:CDS |
| CAGACCCTTACAAGTGTACA+AGG | 0.539307 | 3.3:+52004244 | None:intergenic |
| TCTATGAACGCAAGAAGTAT+GGG | 0.555254 | 3.3:-52004063 | MS.gene048485:CDS |
| CTGCAAGGAATGCACACAGC+AGG | 0.557725 | 3.3:-52004146 | MS.gene048485:CDS |
| GATCAAATCAGGATGATGCT+TGG | 0.567091 | 3.3:+52004354 | None:intergenic |
| TTCCTTGCAGTAGTAAGCAT+CGG | 0.596378 | 3.3:+52004159 | None:intergenic |
| TGCGTTATATGTGGAGGAGT+AGG | 0.603349 | 3.3:-52004187 | MS.gene048485:CDS |
| ATCCGATGCTTACTACTGCA+AGG | 0.604803 | 3.3:-52004161 | MS.gene048485:CDS |
| GCGTCCTTGTACACTTGTAA+GGG | 0.606045 | 3.3:-52004248 | MS.gene048485:CDS |
| CACACAGCAGGAGAAAGATA+GGG | 0.609822 | 3.3:-52004134 | MS.gene048485:CDS |
| GGTCGATGCGTTATATGTGG+AGG | 0.657003 | 3.3:-52004193 | MS.gene048485:CDS |
| CGATTATGTGAGAAATGTGA+CGG | 0.674326 | 3.3:-52004298 | MS.gene048485:CDS |
| CAGCAGGAGAAAGATAGGGA+TGG | 0.676444 | 3.3:-52004130 | MS.gene048485:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATAGAATAAATCAGTTT+TGG | + | chr3.3:52004331-52004350 | None:intergenic | 20.0% |
| ! | ACTCCCTAAATTGACAATTT+TGG | + | chr3.3:52004307-52004326 | None:intergenic | 30.0% |
| ! | CTCCCTAAATTGACAATTTT+GGG | + | chr3.3:52004306-52004325 | None:intergenic | 30.0% |
| ! | TCCCTAAATTGACAATTTTG+GGG | + | chr3.3:52004305-52004324 | None:intergenic | 30.0% |
| ATGCAACTATGGATCATTTC+AGG | - | chr3.3:52004191-52004210 | MS.gene048485:CDS | 35.0% | |
| CGATTATGTGAGAAATGTGA+CGG | - | chr3.3:52004108-52004127 | MS.gene048485:CDS | 35.0% | |
| GCCCCAAAATTGTCAATTTA+GGG | - | chr3.3:52004301-52004320 | MS.gene048485:CDS | 35.0% | |
| TGCAACTATGGATCATTTCA+GGG | - | chr3.3:52004192-52004211 | MS.gene048485:CDS | 35.0% | |
| TGCCCCAAAATTGTCAATTT+AGG | - | chr3.3:52004300-52004319 | MS.gene048485:CDS | 35.0% | |
| TTCTATGAACGCAAGAAGTA+TGG | - | chr3.3:52004342-52004361 | MS.gene048485:CDS | 35.0% | |
| ! | TCTATGAACGCAAGAAGTAT+GGG | - | chr3.3:52004343-52004362 | MS.gene048485:CDS | 35.0% |
| AATCGTCCAATAGCAATTCC+TGG | + | chr3.3:52004093-52004112 | None:intergenic | 40.0% | |
| GATCAAATCAGGATGATGCT+TGG | + | chr3.3:52004055-52004074 | None:intergenic | 40.0% | |
| GTCTGTGATGAATGCAACTA+TGG | - | chr3.3:52004180-52004199 | MS.gene048485:CDS | 40.0% | |
| TTACGGCACATGATCAAATC+AGG | + | chr3.3:52004066-52004085 | None:intergenic | 40.0% | |
| TTCCTTGCAGTAGTAAGCAT+CGG | + | chr3.3:52004250-52004269 | None:intergenic | 40.0% | |
| AAGCAGCCAGGAATTGCTAT+TGG | - | chr3.3:52004084-52004103 | MS.gene048485:CDS | 45.0% | |
| ATCCGATGCTTACTACTGCA+AGG | - | chr3.3:52004245-52004264 | MS.gene048485:CDS | 45.0% | |
| CACACAGCAGGAGAAAGATA+GGG | - | chr3.3:52004272-52004291 | MS.gene048485:CDS | 45.0% | |
| CAGACCCTTACAAGTGTACA+AGG | + | chr3.3:52004165-52004184 | None:intergenic | 45.0% | |
| GCGTCCTTGTACACTTGTAA+GGG | - | chr3.3:52004158-52004177 | MS.gene048485:CDS | 45.0% | |
| TAGCAATTCCTGGCTGCTTA+CGG | + | chr3.3:52004083-52004102 | None:intergenic | 45.0% | |
| TGCGTCCTTGTACACTTGTA+AGG | - | chr3.3:52004157-52004176 | MS.gene048485:CDS | 45.0% | |
| TGCGTTATATGTGGAGGAGT+AGG | - | chr3.3:52004219-52004238 | MS.gene048485:CDS | 45.0% | |
| CAGCAGGAGAAAGATAGGGA+TGG | - | chr3.3:52004276-52004295 | MS.gene048485:CDS | 50.0% | |
| CAGGGTCGATGCGTTATATG+TGG | - | chr3.3:52004210-52004229 | MS.gene048485:CDS | 50.0% | |
| GCACACAGCAGGAGAAAGAT+AGG | - | chr3.3:52004271-52004290 | MS.gene048485:CDS | 50.0% | |
| GGTCGATGCGTTATATGTGG+AGG | - | chr3.3:52004213-52004232 | MS.gene048485:CDS | 50.0% | |
| ATCATGTGCCGTAAGCAGCC+AGG | - | chr3.3:52004072-52004091 | MS.gene048485:CDS | 55.0% | |
| CTGCAAGGAATGCACACAGC+AGG | - | chr3.3:52004260-52004279 | MS.gene048485:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 52004048 | 52004380 | 52004048 | ID=MS.gene048485 |
| chr3.3 | mRNA | 52004048 | 52004380 | 52004048 | ID=MS.gene048485.t1;Parent=MS.gene048485 |
| chr3.3 | exon | 52004048 | 52004380 | 52004048 | ID=MS.gene048485.t1.exon1;Parent=MS.gene048485.t1 |
| chr3.3 | CDS | 52004048 | 52004380 | 52004048 | ID=cds.MS.gene048485.t1;Parent=MS.gene048485.t1 |
| Gene Sequence |
| Protein sequence |
