Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009274.t1 | XP_003598033.2 | 96.6 | 493 | 16 | 1 | 3 | 495 | 49 | 540 | 4.70E-257 | 896.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009274.t1 | Q9FVW4 | 48.2 | 498 | 191 | 12 | 3 | 495 | 47 | 482 | 3.2e-97 | 357.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009274.t1 | G7IYF2 | 96.6 | 493 | 16 | 1 | 3 | 495 | 49 | 540 | 3.4e-257 | 896.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene009274 | MS.gene30557 | PPI |
| MS.gene009274 | MS.gene069519 | PPI |
| MS.gene009274 | MS.gene41129 | PPI |
| MS.gene009274 | MS.gene021158 | PPI |
| MS.gene009274 | MS.gene003999 | PPI |
| MS.gene009274 | MS.gene75866 | PPI |
| MS.gene009274 | MS.gene033864 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009274.t1 | MTR_3g005510 | 96.356 | 494 | 17 | 1 | 2 | 495 | 48 | 540 | 0.0 | 961 |
| MS.gene009274.t1 | MTR_2g005600 | 41.551 | 503 | 235 | 18 | 10 | 495 | 50 | 510 | 3.02e-84 | 269 |
| MS.gene009274.t1 | MTR_2g005600 | 39.587 | 533 | 233 | 18 | 10 | 495 | 50 | 540 | 1.04e-81 | 263 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009274.t1 | AT1G33980 | 48.503 | 501 | 187 | 14 | 2 | 495 | 46 | 482 | 1.81e-121 | 364 |
| MS.gene009274.t1 | AT1G33980 | 48.303 | 501 | 188 | 14 | 2 | 495 | 48 | 484 | 8.78e-121 | 363 |
Find 115 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCCCCGTCTGGTATTAC+TGG | 0.217254 | 3.2:+53496 | None:intergenic |
| GAGTTTGCGGAGTTCTTTAA+TGG | 0.236801 | 3.2:-55958 | MS.gene009274:CDS |
| TTCCACCGGCTTCACGCTTT+TGG | 0.251325 | 3.2:+49201 | None:intergenic |
| AAATTTATATGTGCAGTTAT+AGG | 0.264693 | 3.2:-56035 | None:intergenic |
| GGTAAAAGAAAATGCTTATT+CGG | 0.273466 | 3.2:-48643 | MS.gene009274:CDS |
| ATTCTGAAGGGGAATCTAAA+AGG | 0.281820 | 3.2:-49004 | MS.gene009274:CDS |
| TCTTCTGAATCAAGTATAAA+AGG | 0.294483 | 3.2:-51710 | MS.gene009274:CDS |
| CTTGCCTTGCATAGCAGCTC+TGG | 0.315043 | 3.2:+53526 | None:intergenic |
| AGGCGTCCACCTCGTCCTTT+TGG | 0.319628 | 3.2:-49080 | MS.gene009274:CDS |
| GTCATACCTTCAACTGATTC+AGG | 0.322351 | 3.2:+48795 | None:intergenic |
| TCTGTATGCAGAAACAAGTT+TGG | 0.325968 | 3.2:-48255 | MS.gene009274:intron |
| GCTGCTATGCAAGGCAAGCC+TGG | 0.337012 | 3.2:-53521 | MS.gene009274:CDS |
| CAGGCCTGATCGTGGTGTTT+GGG | 0.338312 | 3.2:-48898 | MS.gene009274:CDS |
| CCATCATCCTTCACTATATA+GGG | 0.349986 | 3.2:+48405 | None:intergenic |
| ATTACTAGAATGTGAAGAAT+TGG | 0.350236 | 3.2:+48860 | None:intergenic |
| ACAGGCCTGATCGTGGTGTT+TGG | 0.351241 | 3.2:-48899 | MS.gene009274:CDS |
| GGCATTGAAGGTTCCGTCTC+TGG | 0.374822 | 3.2:-49704 | MS.gene009274:CDS |
| ACCATCATCCTTCACTATAT+AGG | 0.376532 | 3.2:+48404 | None:intergenic |
| AGGGGAATCTAAAAGGGATT+TGG | 0.379049 | 3.2:-48997 | MS.gene009274:CDS |
| AAAATGCTTATTCGGGAAAC+AGG | 0.394937 | 3.2:-48635 | MS.gene009274:CDS |
| ACGGGGATCTGAAAAGAAAA+AGG | 0.396521 | 3.2:-53483 | MS.gene009274:intron |
| AGAAGAATGGTCCTCACGCC+TGG | 0.398858 | 3.2:+51727 | None:intergenic |
| TGGGTTCAGAAATCATCTTC+AGG | 0.399182 | 3.2:-48235 | MS.gene009274:CDS |
| CTGTATGCAGAAACAAGTTT+GGG | 0.400640 | 3.2:-48254 | MS.gene009274:intron |
| CGTGAAGGGACTATATATAA+AGG | 0.410366 | 3.2:-55228 | MS.gene009274:intron |
| TTGAGGAACTCGAGATAGTC+TGG | 0.416889 | 3.2:+55086 | None:intergenic |
| GTAAAAGAAAATGCTTATTC+GGG | 0.425492 | 3.2:-48642 | MS.gene009274:CDS |
| TCCAAAGCCGAGTACCAAAA+AGG | 0.427328 | 3.2:-55256 | MS.gene009274:CDS |
| TCCGAGTGCAGAAATACAAT+TGG | 0.428949 | 3.2:-55036 | MS.gene009274:CDS |
| CAAATCTTAACTTCTGAGAA+TGG | 0.431636 | 3.2:-49104 | MS.gene009274:CDS |
| AAACTTTCAAAGAAAGGTGT+TGG | 0.453145 | 3.2:-48360 | MS.gene009274:CDS |
| CTTTCAGGCCACTGAAGGTA+TGG | 0.458031 | 3.2:-49282 | MS.gene009274:intron |
| GATCCCCGTCTGGTATTACT+GGG | 0.459889 | 3.2:+53497 | None:intergenic |
| CATCTTGTGTCAGGTTTCAT+CGG | 0.460552 | 3.2:-53573 | MS.gene009274:intron |
| TATACAGTGCATGGCATTGA+AGG | 0.462061 | 3.2:-49716 | MS.gene009274:CDS |
| GAGGCTTTGAAATGAGTTTG+AGG | 0.464705 | 3.2:+55069 | None:intergenic |
| AACTTTCAAAGAAAGGTGTT+GGG | 0.465614 | 3.2:-48359 | MS.gene009274:CDS |
| TTATACTTGATTCAGAAGAA+TGG | 0.467658 | 3.2:+51714 | None:intergenic |
| CTGTTTAACCATACCTTCAG+TGG | 0.475898 | 3.2:+49274 | None:intergenic |
| CGTCTGGTATTACTGGGGCC+AGG | 0.479425 | 3.2:+53503 | None:intergenic |
| TCTGACGTATATATGCCATC+AGG | 0.484252 | 3.2:+54810 | None:intergenic |
| AGAAATACAATTGGAAAGAA+AGG | 0.484703 | 3.2:-55027 | MS.gene009274:CDS |
| CTTTATATATAGTCCCTTCA+CGG | 0.485953 | 3.2:+55229 | None:intergenic |
| GGTGAATTCTCAACTTCTGC+AGG | 0.489502 | 3.2:-48609 | MS.gene009274:CDS |
| GCCATCAGGGGTGTGACAAT+AGG | 0.490885 | 3.2:+54824 | None:intergenic |
| TTCTGAAGGGGAATCTAAAA+GGG | 0.490928 | 3.2:-49003 | MS.gene009274:CDS |
| GCAAACTCGAAAACATCATC+AGG | 0.492867 | 3.2:+55973 | None:intergenic |
| ACAATGTCAAGAATGCTAAT+CGG | 0.510501 | 3.2:-51787 | MS.gene009274:CDS |
| TCATATCCTGAATCAGTTGA+AGG | 0.510630 | 3.2:-48801 | MS.gene009274:intron |
| CAGAACCAAAAGCGTGAAGC+CGG | 0.512531 | 3.2:-49206 | MS.gene009274:CDS |
| AAGCCGGTGGAAGATTGATC+AGG | 0.514442 | 3.2:-49190 | MS.gene009274:CDS |
| GGTGCCCAAACACCACGATC+AGG | 0.517553 | 3.2:+48894 | None:intergenic |
| GAAGAATGGTCCTCACGCCT+GGG | 0.520144 | 3.2:+51728 | None:intergenic |
| TCCAATTGTATTTCTGCACT+CGG | 0.526926 | 3.2:+55035 | None:intergenic |
| TCATCGGCCGCAACAAGAGT+TGG | 0.528330 | 3.2:-53557 | MS.gene009274:CDS |
| GAAGGGAAACTTTCAAAGAA+AGG | 0.528593 | 3.2:-48366 | MS.gene009274:CDS |
| TGCTGTTGACAGTGGTCCAC+TGG | 0.532809 | 3.2:-54783 | MS.gene009274:intron |
| CCCTATATAGTGAAGGATGA+TGG | 0.537091 | 3.2:-48405 | MS.gene009274:CDS |
| AATCCAGACCTTGAACCAAA+AGG | 0.539385 | 3.2:+49065 | None:intergenic |
| CTGCCTTTACTGTGGTCCCC+AGG | 0.539742 | 3.2:-51745 | MS.gene009274:CDS |
| ACGCCTGGGGACCACAGTAA+AGG | 0.540090 | 3.2:+51742 | None:intergenic |
| TGGTTGTCAGTATGTTCAGA+AGG | 0.540774 | 3.2:-51810 | MS.gene009274:intron |
| CTCCTTTCTAGGGGCTAGCA+AGG | 0.541253 | 3.2:-54852 | MS.gene009274:intron |
| TCAACTGATTCAGGATATGA+TGG | 0.541254 | 3.2:+48804 | None:intergenic |
| ACCTATTGTCACACCCCTGA+TGG | 0.547863 | 3.2:-54825 | MS.gene009274:CDS |
| ACTCAACTACTGCTTTATGC+TGG | 0.548532 | 3.2:+55298 | None:intergenic |
| AGTAGTCCCTCTGTTGAGAA+TGG | 0.556313 | 3.2:-48579 | MS.gene009274:intron |
| CTGACGTATATATGCCATCA+GGG | 0.556567 | 3.2:+54811 | None:intergenic |
| CATGTCTCTAGTCATGATGC+TGG | 0.556919 | 3.2:-49035 | MS.gene009274:CDS |
| CCTTCTGAAAGGAAAACAGC+GGG | 0.562206 | 3.2:-49642 | MS.gene009274:CDS |
| AGAGCCAGAGCTGCTATGCA+AGG | 0.562280 | 3.2:-53530 | MS.gene009274:CDS |
| TCTTAACTTCTGAGAATGGA+AGG | 0.562840 | 3.2:-49100 | MS.gene009274:CDS |
| GTGCAGTCAGTTCGAGTGAA+GGG | 0.563790 | 3.2:-48383 | MS.gene009274:CDS |
| GGCTTTGGAACACGCTGAGA+AGG | 0.570985 | 3.2:+55270 | None:intergenic |
| TACTGTGGTCCCCAGGCGTG+AGG | 0.571346 | 3.2:-51738 | MS.gene009274:CDS |
| TGGAACTTTCAGGCCACTGA+AGG | 0.573276 | 3.2:-49287 | MS.gene009274:intron |
| TGGCCCCAGTAATACCAGAC+GGG | 0.576005 | 3.2:-53501 | MS.gene009274:CDS |
| GCTGGGCATGCTAATTCTGA+AGG | 0.580615 | 3.2:-49017 | MS.gene009274:CDS |
| GCTCCTGATCAATCTTCCAC+CGG | 0.582381 | 3.2:+49187 | None:intergenic |
| TGAGCTCTCATCATCCTCAC+TGG | 0.585263 | 3.2:-48838 | MS.gene009274:CDS |
| AGAAAGGAAGCTGAGCAAGC+TGG | 0.587029 | 3.2:-55011 | MS.gene009274:intron |
| CACCAACCATTCTCAACAGA+GGG | 0.588071 | 3.2:+48573 | None:intergenic |
| GCACTCGGAAGATGTTCTTG+AGG | 0.590407 | 3.2:+55050 | None:intergenic |
| GGTGCAGTCAGTTCGAGTGA+AGG | 0.593819 | 3.2:-48384 | MS.gene009274:CDS |
| CTGGGCATGCTAATTCTGAA+GGG | 0.594470 | 3.2:-49016 | MS.gene009274:CDS |
| TCTTCTGCCAACTCTTGTTG+CGG | 0.594967 | 3.2:+53550 | None:intergenic |
| ATGTCTCTAGTCATGATGCT+GGG | 0.599416 | 3.2:-49034 | MS.gene009274:CDS |
| AACCAAAAGCGTGAAGCCGG+TGG | 0.606148 | 3.2:-49203 | MS.gene009274:CDS |
| GAATTCTCAACTTCTGCAGG+TGG | 0.610336 | 3.2:-48606 | MS.gene009274:CDS |
| TTTCCTGAGTCAGAAGTCAG+TGG | 0.613047 | 3.2:+49677 | None:intergenic |
| ATACCACTGACTTCTGACTC+AGG | 0.622853 | 3.2:-49680 | MS.gene009274:CDS |
| CTGGCCCCAGTAATACCAGA+CGG | 0.624233 | 3.2:-53502 | MS.gene009274:CDS |
| GACGTACTAGAAACAAGGAC+AGG | 0.626561 | 3.2:-48917 | MS.gene009274:CDS |
| TGGGGCTCCCTATATAGTGA+AGG | 0.637270 | 3.2:-48412 | MS.gene009274:CDS |
| TAGAATGTGAAGAATTGGAA+CGG | 0.637276 | 3.2:+48865 | None:intergenic |
| ATTGATGAATATACAGTGCA+TGG | 0.638195 | 3.2:-49725 | MS.gene009274:CDS |
| AACAAGGACAGGCCTGATCG+TGG | 0.639778 | 3.2:-48906 | MS.gene009274:CDS |
| AGTCAGTGGTATTCCAGAGA+CGG | 0.644574 | 3.2:+49691 | None:intergenic |
| CAGAAGCGTGCTGTTGACAG+TGG | 0.645146 | 3.2:-54791 | MS.gene009274:CDS |
| TGGGCATGCTAATTCTGAAG+GGG | 0.646561 | 3.2:-49015 | MS.gene009274:CDS |
| GACCTTGAACCAAAAGGACG+AGG | 0.650876 | 3.2:+49071 | None:intergenic |
| TGTTGGGAATAGTACTCATG+AGG | 0.651580 | 3.2:-48343 | MS.gene009274:intron |
| GCTTATTCGGGAAACAGGAG+TGG | 0.655321 | 3.2:-48630 | MS.gene009274:CDS |
| AGAAAGACGTACTAGAAACA+AGG | 0.658009 | 3.2:-48922 | MS.gene009274:CDS |
| AAGAATGGTCCTCACGCCTG+GGG | 0.661060 | 3.2:+51729 | None:intergenic |
| TCCTTCTGAAAGGAAAACAG+CGG | 0.668874 | 3.2:-49643 | MS.gene009274:CDS |
| CTTGAACCAAAAGGACGAGG+TGG | 0.668946 | 3.2:+49074 | None:intergenic |
| ACACCAACCATTCTCAACAG+AGG | 0.678931 | 3.2:+48572 | None:intergenic |
| AGACAAATCTGCCTTTACTG+TGG | 0.679939 | 3.2:-51753 | MS.gene009274:CDS |
| GGCCCCAGTAATACCAGACG+GGG | 0.682231 | 3.2:-53500 | MS.gene009274:CDS |
| GCAGTAGATGTAGACTGACT+CGG | 0.696602 | 3.2:+49140 | None:intergenic |
| GAAGAATTGGAACGGCAAAG+AGG | 0.703570 | 3.2:+48873 | None:intergenic |
| TGACGTATATATGCCATCAG+GGG | 0.713880 | 3.2:+54812 | None:intergenic |
| ATCCCCGTCTGGTATTACTG+GGG | 0.722921 | 3.2:+53498 | None:intergenic |
| TGGTGCAGACTGTGCCAGTG+AGG | 0.729152 | 3.2:+48824 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 48228 | 56049 | 48228 | ID=MS.gene009274 |
| chr3.2 | mRNA | 48228 | 56049 | 48228 | ID=MS.gene009274.t1;Parent=MS.gene009274 |
| chr3.2 | exon | 55935 | 56049 | 55935 | ID=MS.gene009274.t1.exon1;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 55935 | 56049 | 55935 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 55229 | 55324 | 55229 | ID=MS.gene009274.t1.exon2;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 55229 | 55324 | 55229 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 55012 | 55110 | 55012 | ID=MS.gene009274.t1.exon3;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 55012 | 55110 | 55012 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 54784 | 54863 | 54784 | ID=MS.gene009274.t1.exon4;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 54784 | 54863 | 54784 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 53484 | 53582 | 53484 | ID=MS.gene009274.t1.exon5;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 53484 | 53582 | 53484 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 51690 | 51822 | 51690 | ID=MS.gene009274.t1.exon6;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 51690 | 51822 | 51690 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 49631 | 49755 | 49631 | ID=MS.gene009274.t1.exon7;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 49631 | 49755 | 49631 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 48802 | 49297 | 48802 | ID=MS.gene009274.t1.exon8;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 48802 | 49297 | 48802 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 48580 | 48669 | 48580 | ID=MS.gene009274.t1.exon9;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 48580 | 48669 | 48580 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 48344 | 48459 | 48344 | ID=MS.gene009274.t1.exon10;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 48344 | 48459 | 48344 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| chr3.2 | exon | 48228 | 48266 | 48228 | ID=MS.gene009274.t1.exon11;Parent=MS.gene009274.t1 |
| chr3.2 | CDS | 48228 | 48266 | 48228 | ID=cds.MS.gene009274.t1;Parent=MS.gene009274.t1 |
| Gene Sequence |
| Protein sequence |
