AlfalfaGEDB | MS.gene012321

M. sativa cultivar XinJiangDaYe / MS.gene012321

KEGG: K07342
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008320 GO:0015031 GO:0015399 GO:0015450 GO:0015833 GO:0016020 GO:0022804 GO:0022857 GO:0022884 GO:0033036 GO:0042886 GO:0042887 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705 GO:0110165 GO:0140318 GO:1904680
PFAM: PF00584.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012321.t1	RHN57236.1	100	69	0	0	1	69	1	69	5.20E-29	136.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012321.t1	P38385	94.2	69	4	0	1	69	1	69	6.9e-30	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012321.t1	A0A1S3VHV1	100.0	69	0	0	1	69	1	69	3.8e-29	136.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene012321	MS.gene98867	PPI
MS.gene012321	MS.gene81703	PPI
MS.gene012321	MS.gene94163	PPI
MS.gene96323	MS.gene012321	PPI
MS.gene073333	MS.gene012321	PPI
MS.gene012321	MS.gene29736	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012321.t1	MTR_5g084060	100.000	69	0	0	1	69	1	69	2.53e-44	137
MS.gene012321.t1	MTR_4g127150	100.000	69	0	0	1	69	1	69	2.53e-44	137

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene012321.t1	AT5G50460	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene012321.t1	AT4G24920	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene012321.t1	AT3G48570	86.957	69	9	1	69	1	69	2.08e-39	124

Find 16 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAACGATGATGTTGTTAAT+TGG	0.213895	8.4:-7546593	None:intergenic
TCGTATTGTAGAATTCTCTA+AGG	0.363993	8.4:+7546499	MS.gene012321:intron
TGCCATCGGTTTCGTTGTGA+TGG	0.408671	8.4:+7546535	MS.gene012321:CDS
TACCTTTGCGATCGGGTTTG+TGG	0.417825	8.4:-7546392	None:intergenic
AACAACATCATCGTTGGATC+TGG	0.426526	8.4:+7546599	MS.gene012321:CDS
CATCATCGTTGGATCTGGTT+AGG	0.451612	8.4:+7546604	MS.gene012321:CDS
TTCGTTGTGATGGGATTCGT+TGG	0.482699	8.4:+7546545	MS.gene012321:CDS
GCCATCGGTTTCGTTGTGAT+GGG	0.492846	8.4:+7546536	MS.gene012321:CDS
CCAATTAACAACATCATCGT+TGG	0.497266	8.4:+7546593	MS.gene012321:CDS
GTTGCTGTGCGCACTGCCAT+CGG	0.516428	8.4:+7546521	MS.gene012321:CDS
TGGATCGAAAACAGAATCAA+TGG	0.519751	8.4:-7546325	None:intergenic
AATTCGCTAAGGACAGCGTA+AGG	0.605053	8.4:+7546355	MS.gene012321:CDS
TGCCACAAACCCGATCGCAA+AGG	0.641795	8.4:+7546390	MS.gene012321:CDS
TCCCATCACAACGAAACCGA+TGG	0.648968	8.4:-7546537	None:intergenic
TCCACTCAGAGAATTCGCTA+AGG	0.652105	8.4:+7546344	MS.gene012321:CDS
TCCTTAGCGAATTCTCTGAG+TGG	0.675399	8.4:-7546345	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGATCAAAGAGGAATGAAA+GGG	-	chr8.4:7546432-7546451	None:intergenic	30.0%
	TCATAAAACCAAGATCAAAG+AGG	-	chr8.4:7546442-7546461	None:intergenic	30.0%
	TCGTATTGTAGAATTCTCTA+AGG	+	chr8.4:7546499-7546518	MS.gene012321:intron	30.0%
!	TTTTATGAATCTCTGTGCTA+TGG	+	chr8.4:7546454-7546473	MS.gene012321:intron	30.0%
!!!	AGTTTTTTTACCTTTGCGAT+CGG	-	chr8.4:7546403-7546422	None:intergenic	30.0%
	CAAGATCAAAGAGGAATGAA+AGG	-	chr8.4:7546433-7546452	None:intergenic	35.0%
	CCAACGATGATGTTGTTAAT+TGG	-	chr8.4:7546596-7546615	None:intergenic	35.0%
	CCAATTAACAACATCATCGT+TGG	+	chr8.4:7546593-7546612	MS.gene012321:CDS	35.0%
	CTTTCATTCCTCTTTGATCT+TGG	+	chr8.4:7546431-7546450	MS.gene012321:intron	35.0%
	TGGATCGAAAACAGAATCAA+TGG	-	chr8.4:7546328-7546347	None:intergenic	35.0%
!!!	GTTTTTTTACCTTTGCGATC+GGG	-	chr8.4:7546402-7546421	None:intergenic	35.0%
	AACAACATCATCGTTGGATC+TGG	+	chr8.4:7546599-7546618	MS.gene012321:CDS	40.0%
	AATTCGCTAAGGACAGCGTA+AGG	+	chr8.4:7546355-7546374	MS.gene012321:CDS	45.0%
	TCCACTCAGAGAATTCGCTA+AGG	+	chr8.4:7546344-7546363	MS.gene012321:CDS	45.0%
	TCCTTAGCGAATTCTCTGAG+TGG	-	chr8.4:7546348-7546367	None:intergenic	45.0%
!	TTCGTTGTGATGGGATTCGT+TGG	+	chr8.4:7546545-7546564	MS.gene012321:CDS	45.0%
	TACCTTTGCGATCGGGTTTG+TGG	-	chr8.4:7546395-7546414	None:intergenic	50.0%
	TCCCATCACAACGAAACCGA+TGG	-	chr8.4:7546540-7546559	None:intergenic	50.0%
!!	GCCATCGGTTTCGTTGTGAT+GGG	+	chr8.4:7546536-7546555	MS.gene012321:CDS	50.0%
!!	TGCCATCGGTTTCGTTGTGA+TGG	+	chr8.4:7546535-7546554	MS.gene012321:CDS	50.0%
	TGCCACAAACCCGATCGCAA+AGG	+	chr8.4:7546390-7546409	MS.gene012321:CDS	55.0%
	GTTGCTGTGCGCACTGCCAT+CGG	+	chr8.4:7546521-7546540	MS.gene012321:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	7546318	7546625	7546318	ID=MS.gene012321
chr8.4	mRNA	7546318	7546625	7546318	ID=MS.gene012321.t1;Parent=MS.gene012321
chr8.4	exon	7546318	7546411	7546318	ID=MS.gene012321.t1.exon1;Parent=MS.gene012321.t1
chr8.4	CDS	7546318	7546411	7546318	ID=cds.MS.gene012321.t1;Parent=MS.gene012321.t1
chr8.4	exon	7546510	7546625	7546510	ID=MS.gene012321.t1.exon2;Parent=MS.gene012321.t1
chr8.4	CDS	7546510	7546625	7546510	ID=cds.MS.gene012321.t1;Parent=MS.gene012321.t1

Gene Sequence	>MS.gene012321 ATGGACGCCATTGATTCTGTTTTCGATCCACTCAGAGAATTCGCTAAGGACAGCGTAAGGCTCGTCAAGCGATGCCACAAACCCGATCGCAAAGGTAAAAAAACTCAAATTCCCTTTCATTCCTCTTTGATCTTGGTTTTATGAATCTCTGTGCTATGGATCTTTAATTTTATTGTACATTTCGTATTGTAGAATTCTCTAAGGTTGCTGTGCGCACTGCCATCGGTTTCGTTGTGATGGGATTCGTTGGCTTTTTCGTTAAGCTCATTTTCATTCCAATTAACAACATCATCGTTGGATCTGGTTAG
Protein sequence	>MS.gene012321.t1 MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG