Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98867.t1 | RHN57236.1 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 5.20E-29 | 136.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98867.t1 | P38385 | 94.2 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 6.9e-30 | 130.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98867.t1 | A0A1S3VHV1 | 100.0 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 3.8e-29 | 136.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene98867 | 0.818978 | 1.48E-52 | -1.69E-46 |
| MS.gene051490 | MS.gene98867 | 0.80798 | 3.88E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene98867 | 0.806356 | 8.56E-50 | -1.69E-46 |
| MS.gene051832 | MS.gene98867 | 0.831719 | 1.42E-55 | -1.69E-46 |
| MS.gene051848 | MS.gene98867 | -0.804546 | 2.05E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene98867 | 0.801951 | 7.07E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene98867 | 0.800595 | 1.34E-48 | -1.69E-46 |
| MS.gene056699 | MS.gene98867 | 0.812019 | 5.23E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene98867 | 0.825915 | 3.61E-54 | -1.69E-46 |
| MS.gene059170 | MS.gene98867 | 0.805737 | 1.16E-49 | -1.69E-46 |
| MS.gene059774 | MS.gene98867 | 0.80142 | 9.08E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene98867 | -0.833075 | 6.57E-56 | -1.69E-46 |
| MS.gene060365 | MS.gene98867 | 0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene98867 | 0.812308 | 4.53E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene98867 | 0.810473 | 1.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012321 | MS.gene98867 | PPI |
| MS.gene98867 | MS.gene81703 | PPI |
| MS.gene94163 | MS.gene98867 | PPI |
| MS.gene96323 | MS.gene98867 | PPI |
| MS.gene073333 | MS.gene98867 | PPI |
| MS.gene29736 | MS.gene98867 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98867.t1 | MTR_5g084060 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 2.53e-44 | 137 |
| MS.gene98867.t1 | MTR_4g127150 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 2.53e-44 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98867.t1 | AT5G50460 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 4.75e-41 | 128 |
| MS.gene98867.t1 | AT4G24920 | 92.647 | 68 | 5 | 0 | 1 | 68 | 1 | 68 | 4.75e-41 | 128 |
| MS.gene98867.t1 | AT3G48570 | 86.957 | 69 | 9 | 0 | 1 | 69 | 1 | 69 | 2.08e-39 | 124 |
Find 18 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGAGCCTAACGCTGTCTT+TGG | 0.352610 | 5.2:+71886245 | None:intergenic |
| TTTGTTGTGATGGGATTTGT+TGG | 0.396415 | 5.2:-71886069 | MS.gene98867:CDS |
| TACCTTTGCGATCGGGTTTG+TGG | 0.417825 | 5.2:+71886214 | None:intergenic |
| CCAACGATGATGTTGTTGAT+TGG | 0.436566 | 5.2:+71886021 | None:intergenic |
| CATCATCGTTGGATCTGGTT+AGG | 0.451612 | 5.2:-71886010 | None:intergenic |
| GGGGTTATACCTTTGCGATC+GGG | 0.456406 | 5.2:+71886207 | None:intergenic |
| AACAACATCATCGTTGGATC+TGG | 0.457089 | 5.2:-71886015 | MS.gene98867:CDS |
| TGGATCGAAGACGGAATCAA+TGG | 0.467824 | 5.2:+71886281 | None:intergenic |
| AGGGGTTATACCTTTGCGAT+CGG | 0.473227 | 5.2:+71886206 | None:intergenic |
| GTTGCTGTTCGTACCGCGAT+TGG | 0.481738 | 5.2:-71886093 | MS.gene98867:CDS |
| TTGTTGTGATGGGATTTGTT+GGG | 0.484079 | 5.2:-71886068 | MS.gene98867:CDS |
| CGCGGTACGAACAGCAACCT+TGG | 0.492229 | 5.2:+71886098 | None:intergenic |
| TTCTCTGAGTGGATCGAAGA+CGG | 0.515282 | 5.2:+71886272 | None:intergenic |
| CCAATCAACAACATCATCGT+TGG | 0.538152 | 5.2:-71886021 | MS.gene98867:CDS |
| AATTCGCCAAAGACAGCGTT+AGG | 0.569841 | 5.2:-71886251 | MS.gene98867:CDS |
| TGCCACAAACCCGATCGCAA+AGG | 0.648616 | 5.2:-71886216 | MS.gene98867:intron |
| CATCACAACAAAACCAATCG+CGG | 0.679795 | 5.2:+71886080 | None:intergenic |
| TCTTTGGCGAATTCTCTGAG+TGG | 0.681670 | 5.2:+71886261 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATCGAATAGAGAATTTA+GGG | + | chr5.2:71886115-71886134 | None:intergenic | 20.0% |
| !! | TGATTTAAATCTCTATATTG+TGG | - | chr5.2:71886137-71886156 | MS.gene98867:intron | 20.0% |
| ! | AAATCGAATAGAGAATTTAG+GGG | + | chr5.2:71886114-71886133 | None:intergenic | 25.0% |
| ! | GTAAATCGAATAGAGAATTT+AGG | + | chr5.2:71886116-71886135 | None:intergenic | 25.0% |
| !! | TTGTTTTGTAGAATTCTCCA+AGG | - | chr5.2:71886184-71886203 | MS.gene98867:intron | 30.0% |
| ! | TTGTTGTGATGGGATTTGTT+GGG | - | chr5.2:71886231-71886250 | MS.gene98867:CDS | 35.0% |
| ! | TTTGTTGTGATGGGATTTGT+TGG | - | chr5.2:71886230-71886249 | MS.gene98867:CDS | 35.0% |
| AACAACATCATCGTTGGATC+TGG | - | chr5.2:71886284-71886303 | MS.gene98867:CDS | 40.0% | |
| CATCACAACAAAACCAATCG+CGG | + | chr5.2:71886222-71886241 | None:intergenic | 40.0% | |
| CCAACGATGATGTTGTTGAT+TGG | + | chr5.2:71886281-71886300 | None:intergenic | 40.0% | |
| CCAATCAACAACATCATCGT+TGG | - | chr5.2:71886278-71886297 | MS.gene98867:CDS | 40.0% | |
| !!! | GCGATTGGTTTTGTTGTGAT+GGG | - | chr5.2:71886221-71886240 | MS.gene98867:CDS | 40.0% |
| AATTCGCCAAAGACAGCGTT+AGG | - | chr5.2:71886048-71886067 | MS.gene98867:CDS | 45.0% | |
| AGGGGTTATACCTTTGCGAT+CGG | + | chr5.2:71886096-71886115 | None:intergenic | 45.0% | |
| TCTTTGGCGAATTCTCTGAG+TGG | + | chr5.2:71886041-71886060 | None:intergenic | 45.0% | |
| TGGATCGAAGACGGAATCAA+TGG | + | chr5.2:71886021-71886040 | None:intergenic | 45.0% | |
| TTCTCTGAGTGGATCGAAGA+CGG | + | chr5.2:71886030-71886049 | None:intergenic | 45.0% | |
| !!! | CGCGATTGGTTTTGTTGTGA+TGG | - | chr5.2:71886220-71886239 | MS.gene98867:CDS | 45.0% |
| AACGAGCCTAACGCTGTCTT+TGG | + | chr5.2:71886057-71886076 | None:intergenic | 50.0% | |
| GGGGTTATACCTTTGCGATC+GGG | + | chr5.2:71886095-71886114 | None:intergenic | 50.0% | |
| TACCTTTGCGATCGGGTTTG+TGG | + | chr5.2:71886088-71886107 | None:intergenic | 50.0% | |
| TGCCACAAACCCGATCGCAA+AGG | - | chr5.2:71886083-71886102 | MS.gene98867:CDS | 55.0% | |
| ! | GTTGCTGTTCGTACCGCGAT+TGG | - | chr5.2:71886206-71886225 | MS.gene98867:intron | 55.0% |
| CGCGGTACGAACAGCAACCT+TGG | + | chr5.2:71886204-71886223 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 71886011 | 71886310 | 71886011 | ID=MS.gene98867 |
| chr5.2 | mRNA | 71886011 | 71886310 | 71886011 | ID=MS.gene98867.t1;Parent=MS.gene98867 |
| chr5.2 | exon | 71886217 | 71886310 | 71886217 | ID=MS.gene98867.t1.exon1;Parent=MS.gene98867.t1 |
| chr5.2 | CDS | 71886217 | 71886310 | 71886217 | ID=cds.MS.gene98867.t1;Parent=MS.gene98867.t1 |
| chr5.2 | exon | 71886011 | 71886126 | 71886011 | ID=MS.gene98867.t1.exon2;Parent=MS.gene98867.t1 |
| chr5.2 | CDS | 71886011 | 71886126 | 71886011 | ID=cds.MS.gene98867.t1;Parent=MS.gene98867.t1 |
| Gene Sequence |
| Protein sequence |
