AlfalfaGEDB | MS.gene81703

M. sativa cultivar XinJiangDaYe / MS.gene81703

KEGG: K07342
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008320 GO:0015031 GO:0015399 GO:0015450 GO:0015833 GO:0016020 GO:0022804 GO:0022857 GO:0022884 GO:0033036 GO:0042886 GO:0042887 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705 GO:0110165 GO:0140318 GO:1904680
PFAM: PF00584.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81703.t1	RHN57236.1	100	69	0	0	1	69	1	69	5.20E-29	136.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81703.t1	P38385	94.2	69	4	0	1	69	1	69	6.9e-30	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81703.t1	A0A1S3VHV1	100.0	69	0	0	1	69	1	69	3.8e-29	136.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049680	MS.gene81703	0.803292	3.74E-49	-1.69E-46
MS.gene050320	MS.gene81703	0.812193	4.79E-51	-1.69E-46
MS.gene050752	MS.gene81703	0.81271	3.70E-51	-1.69E-46
MS.gene050955	MS.gene81703	0.814911	1.21E-51	-1.69E-46
MS.gene051003	MS.gene81703	0.813657	2.29E-51	-1.69E-46
MS.gene051746	MS.gene81703	0.806979	6.32E-50	-1.69E-46
MS.gene051748	MS.gene81703	0.822201	2.69E-53	-1.69E-46
MS.gene051756	MS.gene81703	0.818355	2.05E-52	-1.69E-46
MS.gene05188	MS.gene81703	0.819613	1.06E-52	-1.69E-46
MS.gene051903	MS.gene81703	0.864894	8.75E-65	-1.69E-46
MS.gene051975	MS.gene81703	0.838941	2.13E-57	-1.69E-46
MS.gene052947	MS.gene81703	0.809214	2.11E-50	-1.69E-46
MS.gene053298	MS.gene81703	0.801015	1.10E-48	-1.69E-46
MS.gene053952	MS.gene81703	0.821853	3.24E-53	-1.69E-46
MS.gene053963	MS.gene81703	0.812821	3.50E-51	-1.69E-46
MS.gene054403	MS.gene81703	0.818453	1.94E-52	-1.69E-46
MS.gene05469	MS.gene81703	0.817705	2.87E-52	-1.69E-46
MS.gene055603	MS.gene81703	0.809275	2.05E-50	-1.69E-46
MS.gene056605	MS.gene81703	0.803309	3.71E-49	-1.69E-46
MS.gene056631	MS.gene81703	0.821694	3.52E-53	-1.69E-46
MS.gene056638	MS.gene81703	0.801841	7.44E-49	-1.69E-46
MS.gene056986	MS.gene81703	0.87567	2.57E-68	-1.69E-46
MS.gene057094	MS.gene81703	0.835607	1.52E-56	-1.69E-46
MS.gene057102	MS.gene81703	0.817911	2.58E-52	-1.69E-46
MS.gene05781	MS.gene81703	0.808345	3.24E-50	-1.69E-46
MS.gene057999	MS.gene81703	0.825972	3.50E-54	-1.69E-46
MS.gene058238	MS.gene81703	0.806827	6.81E-50	-1.69E-46
MS.gene059227	MS.gene81703	0.809447	1.88E-50	-1.69E-46
MS.gene06001	MS.gene81703	0.830928	2.23E-55	-1.69E-46
MS.gene06011	MS.gene81703	0.867867	9.97E-66	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene012321	MS.gene81703	PPI
MS.gene98867	MS.gene81703	PPI
MS.gene073333	MS.gene81703	PPI
MS.gene94163	MS.gene81703	PPI
MS.gene96323	MS.gene81703	PPI
MS.gene29736	MS.gene81703	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81703.t1	MTR_5g084060	100.000	69	0	0	1	69	1	69	2.53e-44	137
MS.gene81703.t1	MTR_4g127150	100.000	69	0	0	1	69	1	69	2.53e-44	137

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81703.t1	AT5G50460	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene81703.t1	AT4G24920	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene81703.t1	AT3G48570	86.957	69	9	1	69	1	69	2.08e-39	124

Find 18 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGAGCCTAACGCTGTCTT+TGG	0.352610	5.1:+66204548	None:intergenic
TACCTTTGCGATCGGGTTTG+TGG	0.417825	5.1:+66204517	None:intergenic
CCAACGATGATGTTGTTGAT+TGG	0.436566	5.1:+66204324	None:intergenic
CATCATCGTTGGATCTGGTT+AGG	0.451612	5.1:-66204313	None:intergenic
GGGGTTATACCTTTGCGATC+GGG	0.456406	5.1:+66204510	None:intergenic
AACAACATCATCGTTGGATC+TGG	0.457089	5.1:-66204318	MS.gene81703:CDS
TGGATCGAAGACGGAATCAA+TGG	0.467824	5.1:+66204584	None:intergenic
AGGGGTTATACCTTTGCGAT+CGG	0.473227	5.1:+66204509	None:intergenic
TTTGTTGTGATGGGATTCGT+TGG	0.478676	5.1:-66204372	MS.gene81703:CDS
GTTGCTGTTCGTACCGCGAT+TGG	0.481738	5.1:-66204396	MS.gene81703:CDS
CGCGGTACGAACAGCAACCT+TGG	0.492229	5.1:+66204401	None:intergenic
TTCTCTGAGTGGATCGAAGA+CGG	0.515282	5.1:+66204575	None:intergenic
TTGTTGTGATGGGATTCGTT+GGG	0.527754	5.1:-66204371	MS.gene81703:CDS
CCAATCAACAACATCATCGT+TGG	0.538152	5.1:-66204324	MS.gene81703:CDS
AATTCGCCAAAGACAGCGTT+AGG	0.569841	5.1:-66204554	MS.gene81703:CDS
TGCCACAAACCCGATCGCAA+AGG	0.648616	5.1:-66204519	MS.gene81703:intron
CATCACAACAAAACCAATCG+CGG	0.679795	5.1:+66204383	None:intergenic
TCTTTGGCGAATTCTCTGAG+TGG	0.681670	5.1:+66204564	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATCGAATAGAGAATTTA+GGG	+	chr5.1:66204418-66204437	None:intergenic	20.0%
!!	TGATTTAAATCTCTATATTG+TGG	-	chr5.1:66204440-66204459	MS.gene81703:intron	20.0%
!	AAATCGAATAGAGAATTTAG+GGG	+	chr5.1:66204417-66204436	None:intergenic	25.0%
!	GTAAATCGAATAGAGAATTT+AGG	+	chr5.1:66204419-66204438	None:intergenic	25.0%
!!	TTGTTTTGTAGAATTCTCCA+AGG	-	chr5.1:66204487-66204506	MS.gene81703:intron	30.0%
	AACAACATCATCGTTGGATC+TGG	-	chr5.1:66204587-66204606	MS.gene81703:CDS	40.0%
	CATCACAACAAAACCAATCG+CGG	+	chr5.1:66204525-66204544	None:intergenic	40.0%
	CCAACGATGATGTTGTTGAT+TGG	+	chr5.1:66204584-66204603	None:intergenic	40.0%
	CCAATCAACAACATCATCGT+TGG	-	chr5.1:66204581-66204600	MS.gene81703:CDS	40.0%
!	TTGTTGTGATGGGATTCGTT+GGG	-	chr5.1:66204534-66204553	MS.gene81703:CDS	40.0%
!	TTTGTTGTGATGGGATTCGT+TGG	-	chr5.1:66204533-66204552	MS.gene81703:CDS	40.0%
!!!	GCGATTGGTTTTGTTGTGAT+GGG	-	chr5.1:66204524-66204543	MS.gene81703:CDS	40.0%
	AATTCGCCAAAGACAGCGTT+AGG	-	chr5.1:66204351-66204370	MS.gene81703:CDS	45.0%
	AGGGGTTATACCTTTGCGAT+CGG	+	chr5.1:66204399-66204418	None:intergenic	45.0%
	TCTTTGGCGAATTCTCTGAG+TGG	+	chr5.1:66204344-66204363	None:intergenic	45.0%
	TGGATCGAAGACGGAATCAA+TGG	+	chr5.1:66204324-66204343	None:intergenic	45.0%
	TTCTCTGAGTGGATCGAAGA+CGG	+	chr5.1:66204333-66204352	None:intergenic	45.0%
!!!	CGCGATTGGTTTTGTTGTGA+TGG	-	chr5.1:66204523-66204542	MS.gene81703:CDS	45.0%
	AACGAGCCTAACGCTGTCTT+TGG	+	chr5.1:66204360-66204379	None:intergenic	50.0%
	GGGGTTATACCTTTGCGATC+GGG	+	chr5.1:66204398-66204417	None:intergenic	50.0%
	TACCTTTGCGATCGGGTTTG+TGG	+	chr5.1:66204391-66204410	None:intergenic	50.0%
	TGCCACAAACCCGATCGCAA+AGG	-	chr5.1:66204386-66204405	MS.gene81703:CDS	55.0%
!	GTTGCTGTTCGTACCGCGAT+TGG	-	chr5.1:66204509-66204528	MS.gene81703:intron	55.0%
	CGCGGTACGAACAGCAACCT+TGG	+	chr5.1:66204507-66204526	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	66204314	66204613	66204314	ID=MS.gene81703
chr5.1	mRNA	66204314	66204613	66204314	ID=MS.gene81703.t1;Parent=MS.gene81703
chr5.1	exon	66204520	66204613	66204520	ID=MS.gene81703.t1.exon1;Parent=MS.gene81703.t1
chr5.1	CDS	66204520	66204613	66204520	ID=cds.MS.gene81703.t1;Parent=MS.gene81703.t1
chr5.1	exon	66204314	66204429	66204314	ID=MS.gene81703.t1.exon2;Parent=MS.gene81703.t1
chr5.1	CDS	66204314	66204429	66204314	ID=cds.MS.gene81703.t1;Parent=MS.gene81703.t1

Gene Sequence	>MS.gene81703 ATGGACGCCATTGATTCCGTCTTCGATCCACTCAGAGAATTCGCCAAAGACAGCGTTAGGCTCGTTAAGCGTTGCCACAAACCCGATCGCAAAGGTATAACCCCTAAATTCTCTATTCGATTTACTTGATTTAAATCTCTATATTGTGGATCTTGAATTTTGATTGTGTTTTTTTGTTTTGTAGAATTCTCCAAGGTTGCTGTTCGTACCGCGATTGGTTTTGTTGTGATGGGATTCGTTGGGTTTTTCGTTAAGCTTATCTTTATTCCAATCAACAACATCATCGTTGGATCTGGTTAG
Protein sequence	>MS.gene81703.t1 MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG