AlfalfaGEDB | MS.gene29736

M. sativa cultivar XinJiangDaYe / MS.gene29736

KEGG: K07342
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008320 GO:0015031 GO:0015399 GO:0015450 GO:0015833 GO:0016020 GO:0022804 GO:0022857 GO:0022884 GO:0033036 GO:0042886 GO:0042887 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705 GO:0110165 GO:0140318 GO:1904680
PFAM: PF00584.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29736.t1	RHN57236.1	100	69	0	0	1	69	1	69	5.20E-29	136.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29736.t1	P38385	94.2	69	4	0	1	69	1	69	6.9e-30	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29736.t1	A0A1S3VHV1	100.0	69	0	0	1	69	1	69	3.8e-29	136.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050288	MS.gene29736	0.804605	1.99E-49	-1.69E-46
MS.gene050320	MS.gene29736	0.826479	2.65E-54	-1.69E-46
MS.gene050752	MS.gene29736	0.853385	2.47E-61	-1.69E-46
MS.gene050838	MS.gene29736	0.804113	2.52E-49	-1.69E-46
MS.gene050955	MS.gene29736	0.834603	2.72E-56	-1.69E-46
MS.gene051003	MS.gene29736	0.818541	1.86E-52	-1.69E-46
MS.gene051746	MS.gene29736	0.814233	1.71E-51	-1.69E-46
MS.gene051748	MS.gene29736	0.816143	6.44E-52	-1.69E-46
MS.gene051756	MS.gene29736	0.811505	6.77E-51	-1.69E-46
MS.gene051903	MS.gene29736	0.839438	1.58E-57	-1.69E-46
MS.gene051975	MS.gene29736	0.803507	3.37E-49	-1.69E-46
MS.gene053058	MS.gene29736	0.812259	4.64E-51	-1.69E-46
MS.gene053109	MS.gene29736	0.832323	1.01E-55	-1.69E-46
MS.gene053564	MS.gene29736	0.818331	2.07E-52	-1.69E-46
MS.gene053952	MS.gene29736	0.844294	8.27E-59	-1.69E-46
MS.gene054403	MS.gene29736	0.852491	4.46E-61	-1.69E-46
MS.gene05469	MS.gene29736	0.857529	1.53E-62	-1.69E-46
MS.gene056631	MS.gene29736	0.808185	3.51E-50	-1.69E-46
MS.gene056787	MS.gene29736	0.801778	7.67E-49	-1.69E-46
MS.gene056986	MS.gene29736	0.810122	1.35E-50	-1.69E-46
MS.gene057094	MS.gene29736	0.820361	7.14E-53	-1.69E-46
MS.gene057234	MS.gene29736	0.803089	4.12E-49	-1.69E-46
MS.gene057999	MS.gene29736	0.814205	1.73E-51	-1.69E-46
MS.gene058238	MS.gene29736	0.807995	3.85E-50	-1.69E-46
MS.gene06001	MS.gene29736	0.812186	4.81E-51	-1.69E-46
MS.gene06011	MS.gene29736	0.85217	5.50E-61	-1.69E-46
MS.gene060763	MS.gene29736	0.816404	5.63E-52	-1.69E-46
MS.gene061145	MS.gene29736	0.803685	3.10E-49	-1.69E-46
MS.gene06118	MS.gene29736	0.818679	1.73E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene073333	MS.gene29736	PPI
MS.gene94163	MS.gene29736	PPI
MS.gene96323	MS.gene29736	PPI
MS.gene29736	MS.gene98867	PPI
MS.gene29736	MS.gene81703	PPI
MS.gene012321	MS.gene29736	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29736.t1	MTR_5g084060	100.000	69	0	0	1	69	1	69	2.53e-44	137
MS.gene29736.t1	MTR_4g127150	100.000	69	0	0	1	69	1	69	2.53e-44	137

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29736.t1	AT5G50460	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene29736.t1	AT4G24920	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene29736.t1	AT3G48570	86.957	69	9	1	69	1	69	2.08e-39	124

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGAGCCTAACGCTGTCTT+TGG	0.352610	5.3:+68151451	None:intergenic
CGCGATTGGTTTCGTTGTGA+TGG	0.380019	5.3:-68151286	MS.gene29736:CDS
TTCGTTGTGATGGGATTTGT+TGG	0.388700	5.3:-68151276	MS.gene29736:CDS
CACCTTTGCGATCGGGTTTG+TGG	0.390413	5.3:+68151420	None:intergenic
CCAACGATGATGTTGTTGAT+TGG	0.436566	5.3:+68151228	None:intergenic
GGGGTTACACCTTTGCGATC+GGG	0.438874	5.3:+68151413	None:intergenic
CATCATCGTTGGATCTGGTT+AGG	0.451612	5.3:-68151217	None:intergenic
AACAACATCATCGTTGGATC+TGG	0.457089	5.3:-68151222	MS.gene29736:CDS
AGGGGTTACACCTTTGCGAT+CGG	0.459137	5.3:+68151412	None:intergenic
TGGATCGAAGACGGAATCAA+TGG	0.467824	5.3:+68151487	None:intergenic
GTTGCTGTTCGTACCGCGAT+TGG	0.481738	5.3:-68151300	MS.gene29736:CDS
TCGTTGTGATGGGATTTGTT+GGG	0.487876	5.3:-68151275	MS.gene29736:CDS
GCGATTGGTTTCGTTGTGAT+GGG	0.488493	5.3:-68151285	MS.gene29736:CDS
CGCGGTACGAACAGCAACCT+TGG	0.492229	5.3:+68151305	None:intergenic
TTCTCTGAGTGGATCGAAGA+CGG	0.515282	5.3:+68151478	None:intergenic
CCAATCAACAACATCATCGT+TGG	0.538152	5.3:-68151228	MS.gene29736:CDS
AATTCGCCAAAGACAGCGTT+AGG	0.569841	5.3:-68151457	MS.gene29736:CDS
TGCCACAAACCCGATCGCAA+AGG	0.641778	5.3:-68151422	MS.gene29736:intron
CATCACAACGAAACCAATCG+CGG	0.678324	5.3:+68151287	None:intergenic
TCTTTGGCGAATTCTCTGAG+TGG	0.681670	5.3:+68151467	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTGAAGAGAAAATCGATTT+AGG	+	chr5.3:68151323-68151342	None:intergenic	30.0%
!	GTGAAGAGAAAATCGATTTA+GGG	+	chr5.3:68151322-68151341	None:intergenic	30.0%
!	TGAAGAGAAAATCGATTTAG+GGG	+	chr5.3:68151321-68151340	None:intergenic	30.0%
!	TGATTTTGTAGAATTCTCCA+AGG	-	chr5.3:68151390-68151409	MS.gene29736:intron	30.0%
	AACAACATCATCGTTGGATC+TGG	-	chr5.3:68151490-68151509	MS.gene29736:CDS	40.0%
	CCAACGATGATGTTGTTGAT+TGG	+	chr5.3:68151487-68151506	None:intergenic	40.0%
	CCAATCAACAACATCATCGT+TGG	-	chr5.3:68151484-68151503	MS.gene29736:CDS	40.0%
!	TCGTTGTGATGGGATTTGTT+GGG	-	chr5.3:68151437-68151456	MS.gene29736:CDS	40.0%
!	TTCGTTGTGATGGGATTTGT+TGG	-	chr5.3:68151436-68151455	MS.gene29736:CDS	40.0%
	AATTCGCCAAAGACAGCGTT+AGG	-	chr5.3:68151255-68151274	MS.gene29736:CDS	45.0%
	CATCACAACGAAACCAATCG+CGG	+	chr5.3:68151428-68151447	None:intergenic	45.0%
	TCTTTGGCGAATTCTCTGAG+TGG	+	chr5.3:68151248-68151267	None:intergenic	45.0%
	TGGATCGAAGACGGAATCAA+TGG	+	chr5.3:68151228-68151247	None:intergenic	45.0%
	TTCTCTGAGTGGATCGAAGA+CGG	+	chr5.3:68151237-68151256	None:intergenic	45.0%
!!	GCGATTGGTTTCGTTGTGAT+GGG	-	chr5.3:68151427-68151446	MS.gene29736:CDS	45.0%
	AACGAGCCTAACGCTGTCTT+TGG	+	chr5.3:68151264-68151283	None:intergenic	50.0%
	AGGGGTTACACCTTTGCGAT+CGG	+	chr5.3:68151303-68151322	None:intergenic	50.0%
!!	CGCGATTGGTTTCGTTGTGA+TGG	-	chr5.3:68151426-68151445	MS.gene29736:CDS	50.0%
	CACCTTTGCGATCGGGTTTG+TGG	+	chr5.3:68151295-68151314	None:intergenic	55.0%
	GGGGTTACACCTTTGCGATC+GGG	+	chr5.3:68151302-68151321	None:intergenic	55.0%
	TGCCACAAACCCGATCGCAA+AGG	-	chr5.3:68151290-68151309	MS.gene29736:CDS	55.0%
!	GTTGCTGTTCGTACCGCGAT+TGG	-	chr5.3:68151412-68151431	MS.gene29736:intron	55.0%
	CGCGGTACGAACAGCAACCT+TGG	+	chr5.3:68151410-68151429	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	68151218	68151516	68151218	ID=MS.gene29736
chr5.3	mRNA	68151218	68151516	68151218	ID=MS.gene29736.t1;Parent=MS.gene29736
chr5.3	exon	68151423	68151516	68151423	ID=MS.gene29736.t1.exon1;Parent=MS.gene29736.t1
chr5.3	CDS	68151423	68151516	68151423	ID=cds.MS.gene29736.t1;Parent=MS.gene29736.t1
chr5.3	exon	68151218	68151333	68151218	ID=MS.gene29736.t1.exon2;Parent=MS.gene29736.t1
chr5.3	CDS	68151218	68151333	68151218	ID=cds.MS.gene29736.t1;Parent=MS.gene29736.t1

Gene Sequence	>MS.gene29736 ATGGACGCCATTGATTCCGTCTTCGATCCACTCAGAGAATTCGCCAAAGACAGCGTTAGGCTCGTTAAGCGTTGCCACAAACCCGATCGCAAAGGTGTAACCCCTAAATCGATTTTCTCTTCACTTGATTTCAATCTCTATATTGTAGATCTTGAATCTTGATTGTGTTTTTTGATTTTGTAGAATTCTCCAAGGTTGCTGTTCGTACCGCGATTGGTTTCGTTGTGATGGGATTTGTTGGGTTTTTCGTTAAGCTTATCTTTATTCCAATCAACAACATCATCGTTGGATCTGGTTAG
Protein sequence	>MS.gene29736.t1 MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG