AlfalfaGEDB | MS.gene96323

M. sativa cultivar XinJiangDaYe / MS.gene96323

KEGG: K07342
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008320 GO:0015031 GO:0015399 GO:0015450 GO:0015833 GO:0016020 GO:0022804 GO:0022857 GO:0022884 GO:0033036 GO:0042886 GO:0042887 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705 GO:0110165 GO:0140318 GO:1904680
PFAM: PF00584.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96323.t1	RHN57236.1	100	69	0	0	1	69	1	69	5.20E-29	136.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96323.t1	P38385	94.2	69	4	0	1	69	1	69	6.9e-30	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96323.t1	A0A1S3VHV1	100.0	69	0	0	1	69	1	69	3.8e-29	136.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene96323	MS.gene94163	PPI
MS.gene96323	MS.gene012321	PPI
MS.gene96323	MS.gene29736	PPI
MS.gene96323	MS.gene98867	PPI
MS.gene96323	MS.gene81703	PPI
MS.gene96323	MS.gene073333	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96323.t1	MTR_5g084060	100.000	69	0	0	1	69	1	69	2.53e-44	137
MS.gene96323.t1	MTR_4g127150	100.000	69	0	0	1	69	1	69	2.53e-44	137

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96323.t1	AT5G50460	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene96323.t1	AT4G24920	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene96323.t1	AT3G48570	86.957	69	9	1	69	1	69	2.08e-39	124

Find 16 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAACGATGATGTTGTTAAT+TGG	0.213895	8.3:-3829754	None:intergenic
TCGTATTGTAGAATTCTCTA+AGG	0.363993	8.3:+3829660	MS.gene96323:intron
TGCCATCGGTTTCGTTGTGA+TGG	0.408671	8.3:+3829696	MS.gene96323:CDS
TACCTTTGCGATCGGGTTTG+TGG	0.417825	8.3:-3829553	None:intergenic
AACAACATCATCGTTGGATC+TGG	0.426526	8.3:+3829760	MS.gene96323:CDS
CATCATCGTTGGATCTGGTT+AGG	0.451612	8.3:+3829765	MS.gene96323:CDS
TTCGTTGTGATGGGATTCGT+TGG	0.482699	8.3:+3829706	MS.gene96323:CDS
GCCATCGGTTTCGTTGTGAT+GGG	0.492846	8.3:+3829697	MS.gene96323:CDS
CCAATTAACAACATCATCGT+TGG	0.497266	8.3:+3829754	MS.gene96323:CDS
GTTGCTGTGCGCACTGCCAT+CGG	0.516428	8.3:+3829682	MS.gene96323:CDS
TGGATCGAAAACAGAATCAA+TGG	0.519751	8.3:-3829486	None:intergenic
AATTCGCTAAGGACAGCGTA+AGG	0.605053	8.3:+3829516	MS.gene96323:CDS
TGCCACAAACCCGATCGCAA+AGG	0.641795	8.3:+3829551	MS.gene96323:CDS
TCCCATCACAACGAAACCGA+TGG	0.648968	8.3:-3829698	None:intergenic
TCCACTCAGAGAATTCGCTA+AGG	0.652105	8.3:+3829505	MS.gene96323:CDS
TCCTTAGCGAATTCTCTGAG+TGG	0.675399	8.3:-3829506	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGATCAAAGAGGAATGAAA+GGG	-	chr8.3:3829593-3829612	None:intergenic	30.0%
	TCATAAAACCAAGATCAAAG+AGG	-	chr8.3:3829603-3829622	None:intergenic	30.0%
	TCGTATTGTAGAATTCTCTA+AGG	+	chr8.3:3829660-3829679	MS.gene96323:intron	30.0%
!	TTTTATGAATCTCTGTGCTA+TGG	+	chr8.3:3829615-3829634	MS.gene96323:intron	30.0%
!!!	AGTTTTTTTACCTTTGCGAT+CGG	-	chr8.3:3829564-3829583	None:intergenic	30.0%
	CAAGATCAAAGAGGAATGAA+AGG	-	chr8.3:3829594-3829613	None:intergenic	35.0%
	CCAACGATGATGTTGTTAAT+TGG	-	chr8.3:3829757-3829776	None:intergenic	35.0%
	CCAATTAACAACATCATCGT+TGG	+	chr8.3:3829754-3829773	MS.gene96323:CDS	35.0%
	CTTTCATTCCTCTTTGATCT+TGG	+	chr8.3:3829592-3829611	MS.gene96323:intron	35.0%
	TGGATCGAAAACAGAATCAA+TGG	-	chr8.3:3829489-3829508	None:intergenic	35.0%
!!!	GTTTTTTTACCTTTGCGATC+GGG	-	chr8.3:3829563-3829582	None:intergenic	35.0%
	AACAACATCATCGTTGGATC+TGG	+	chr8.3:3829760-3829779	MS.gene96323:CDS	40.0%
	AATTCGCTAAGGACAGCGTA+AGG	+	chr8.3:3829516-3829535	MS.gene96323:CDS	45.0%
	TCCACTCAGAGAATTCGCTA+AGG	+	chr8.3:3829505-3829524	MS.gene96323:CDS	45.0%
	TCCTTAGCGAATTCTCTGAG+TGG	-	chr8.3:3829509-3829528	None:intergenic	45.0%
!	TTCGTTGTGATGGGATTCGT+TGG	+	chr8.3:3829706-3829725	MS.gene96323:CDS	45.0%
	TACCTTTGCGATCGGGTTTG+TGG	-	chr8.3:3829556-3829575	None:intergenic	50.0%
	TCCCATCACAACGAAACCGA+TGG	-	chr8.3:3829701-3829720	None:intergenic	50.0%
!!	GCCATCGGTTTCGTTGTGAT+GGG	+	chr8.3:3829697-3829716	MS.gene96323:CDS	50.0%
!!	TGCCATCGGTTTCGTTGTGA+TGG	+	chr8.3:3829696-3829715	MS.gene96323:CDS	50.0%
	TGCCACAAACCCGATCGCAA+AGG	+	chr8.3:3829551-3829570	MS.gene96323:CDS	55.0%
	GTTGCTGTGCGCACTGCCAT+CGG	+	chr8.3:3829682-3829701	MS.gene96323:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	3829479	3829786	3829479	ID=MS.gene96323
chr8.3	mRNA	3829479	3829786	3829479	ID=MS.gene96323.t1;Parent=MS.gene96323
chr8.3	exon	3829479	3829572	3829479	ID=MS.gene96323.t1.exon1;Parent=MS.gene96323.t1
chr8.3	CDS	3829479	3829572	3829479	ID=cds.MS.gene96323.t1;Parent=MS.gene96323.t1
chr8.3	exon	3829671	3829786	3829671	ID=MS.gene96323.t1.exon2;Parent=MS.gene96323.t1
chr8.3	CDS	3829671	3829786	3829671	ID=cds.MS.gene96323.t1;Parent=MS.gene96323.t1

Gene Sequence	>MS.gene96323 ATGGACGCCATTGATTCTGTTTTCGATCCACTCAGAGAATTCGCTAAGGACAGCGTAAGGCTCGTCAAGCGATGCCACAAACCCGATCGCAAAGGTAAAAAAACTCAAATTCCCTTTCATTCCTCTTTGATCTTGGTTTTATGAATCTCTGTGCTATGGATCTTTAATTTTATTGTACATTTCGTATTGTAGAATTCTCTAAGGTTGCTGTGCGCACTGCCATCGGTTTCGTTGTGATGGGATTCGTTGGCTTTTTCGTTAAGCTCATTTTCATTCCAATTAACAACATCATCGTTGGATCTGGTTAG
Protein sequence	>MS.gene96323.t1 MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG