AlfalfaGEDB | MS.gene94163

M. sativa cultivar XinJiangDaYe / MS.gene94163

KEGG: K07342
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008320 GO:0015031 GO:0015399 GO:0015450 GO:0015833 GO:0016020 GO:0022804 GO:0022857 GO:0022884 GO:0033036 GO:0042886 GO:0042887 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705 GO:0110165 GO:0140318 GO:1904680
PFAM: PF00584.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94163.t1	RHN57236.1	100	69	0	0	1	69	1	69	5.20E-29	136.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94163.t1	P38385	94.2	69	4	0	1	69	1	69	6.9e-30	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94163.t1	A0A1S3VHV1	100.0	69	0	0	1	69	1	69	3.8e-29	136.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene96323	MS.gene94163	PPI
MS.gene073333	MS.gene94163	PPI
MS.gene012321	MS.gene94163	PPI
MS.gene94163	MS.gene98867	PPI
MS.gene94163	MS.gene29736	PPI
MS.gene94163	MS.gene81703	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94163.t1	MTR_5g084060	100.000	69	0	0	1	69	1	69	2.53e-44	137
MS.gene94163.t1	MTR_4g127150	100.000	69	0	0	1	69	1	69	2.53e-44	137

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94163.t1	AT5G50460	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene94163.t1	AT4G24920	92.647	68	5	1	68	1	68	4.75e-41	128
MS.gene94163.t1	AT3G48570	86.957	69	9	1	69	1	69	2.08e-39	124

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGAGCCTAACGCTGTCTT+TGG	0.352610	5.4:+66594436	None:intergenic
CGCGATTGGTTTCGTTGTGA+TGG	0.380019	5.4:-66594271	MS.gene94163:CDS
TTCGTTGTGATGGGATTTGT+TGG	0.388700	5.4:-66594261	MS.gene94163:CDS
CACCTTTGCGATCGGGTTTG+TGG	0.390413	5.4:+66594405	None:intergenic
CCAACGATGATGTTGTTGAT+TGG	0.436566	5.4:+66594213	None:intergenic
GGGGTTACACCTTTGCGATC+GGG	0.438874	5.4:+66594398	None:intergenic
CATCATCGTTGGATCTGGTT+AGG	0.451612	5.4:-66594202	None:intergenic
AACAACATCATCGTTGGATC+TGG	0.457089	5.4:-66594207	MS.gene94163:CDS
AGGGGTTACACCTTTGCGAT+CGG	0.459137	5.4:+66594397	None:intergenic
TGGATCGAAGACGGAATCAA+TGG	0.467824	5.4:+66594472	None:intergenic
GTTGCTGTTCGTACCGCGAT+TGG	0.481738	5.4:-66594285	MS.gene94163:CDS
TCGTTGTGATGGGATTTGTT+GGG	0.487876	5.4:-66594260	MS.gene94163:CDS
GCGATTGGTTTCGTTGTGAT+GGG	0.488493	5.4:-66594270	MS.gene94163:CDS
CGCGGTACGAACAGCAACCT+TGG	0.492229	5.4:+66594290	None:intergenic
TTCTCTGAGTGGATCGAAGA+CGG	0.515282	5.4:+66594463	None:intergenic
CCAATCAACAACATCATCGT+TGG	0.538152	5.4:-66594213	MS.gene94163:CDS
AATTCGCCAAAGACAGCGTT+AGG	0.569841	5.4:-66594442	MS.gene94163:CDS
TGCCACAAACCCGATCGCAA+AGG	0.641778	5.4:-66594407	MS.gene94163:intron
CATCACAACGAAACCAATCG+CGG	0.678324	5.4:+66594272	None:intergenic
TCTTTGGCGAATTCTCTGAG+TGG	0.681670	5.4:+66594452	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTGAAGAGAAAATCGATTT+AGG	+	chr5.4:66594308-66594327	None:intergenic	30.0%
!	GTGAAGAGAAAATCGATTTA+GGG	+	chr5.4:66594307-66594326	None:intergenic	30.0%
!	TGAAGAGAAAATCGATTTAG+GGG	+	chr5.4:66594306-66594325	None:intergenic	30.0%
!	TGATTTTGTAGAATTCTCCA+AGG	-	chr5.4:66594375-66594394	MS.gene94163:intron	30.0%
	AACAACATCATCGTTGGATC+TGG	-	chr5.4:66594475-66594494	MS.gene94163:CDS	40.0%
	CCAACGATGATGTTGTTGAT+TGG	+	chr5.4:66594472-66594491	None:intergenic	40.0%
	CCAATCAACAACATCATCGT+TGG	-	chr5.4:66594469-66594488	MS.gene94163:CDS	40.0%
!	TCGTTGTGATGGGATTTGTT+GGG	-	chr5.4:66594422-66594441	MS.gene94163:CDS	40.0%
!	TTCGTTGTGATGGGATTTGT+TGG	-	chr5.4:66594421-66594440	MS.gene94163:CDS	40.0%
	AATTCGCCAAAGACAGCGTT+AGG	-	chr5.4:66594240-66594259	MS.gene94163:CDS	45.0%
	CATCACAACGAAACCAATCG+CGG	+	chr5.4:66594413-66594432	None:intergenic	45.0%
	TCTTTGGCGAATTCTCTGAG+TGG	+	chr5.4:66594233-66594252	None:intergenic	45.0%
	TGGATCGAAGACGGAATCAA+TGG	+	chr5.4:66594213-66594232	None:intergenic	45.0%
	TTCTCTGAGTGGATCGAAGA+CGG	+	chr5.4:66594222-66594241	None:intergenic	45.0%
!!	GCGATTGGTTTCGTTGTGAT+GGG	-	chr5.4:66594412-66594431	MS.gene94163:CDS	45.0%
	AACGAGCCTAACGCTGTCTT+TGG	+	chr5.4:66594249-66594268	None:intergenic	50.0%
	AGGGGTTACACCTTTGCGAT+CGG	+	chr5.4:66594288-66594307	None:intergenic	50.0%
!!	CGCGATTGGTTTCGTTGTGA+TGG	-	chr5.4:66594411-66594430	MS.gene94163:CDS	50.0%
	CACCTTTGCGATCGGGTTTG+TGG	+	chr5.4:66594280-66594299	None:intergenic	55.0%
	GGGGTTACACCTTTGCGATC+GGG	+	chr5.4:66594287-66594306	None:intergenic	55.0%
	TGCCACAAACCCGATCGCAA+AGG	-	chr5.4:66594275-66594294	MS.gene94163:CDS	55.0%
!	GTTGCTGTTCGTACCGCGAT+TGG	-	chr5.4:66594397-66594416	MS.gene94163:intron	55.0%
	CGCGGTACGAACAGCAACCT+TGG	+	chr5.4:66594395-66594414	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	66594203	66594501	66594203	ID=MS.gene94163
chr5.4	mRNA	66594203	66594501	66594203	ID=MS.gene94163.t1;Parent=MS.gene94163
chr5.4	exon	66594408	66594501	66594408	ID=MS.gene94163.t1.exon1;Parent=MS.gene94163.t1
chr5.4	CDS	66594408	66594501	66594408	ID=cds.MS.gene94163.t1;Parent=MS.gene94163.t1
chr5.4	exon	66594203	66594318	66594203	ID=MS.gene94163.t1.exon2;Parent=MS.gene94163.t1
chr5.4	CDS	66594203	66594318	66594203	ID=cds.MS.gene94163.t1;Parent=MS.gene94163.t1

Gene Sequence	>MS.gene94163 ATGGACGCCATTGATTCCGTCTTCGATCCACTCAGAGAATTCGCCAAAGACAGCGTTAGGCTCGTTAAGCGTTGCCACAAACCCGATCGCAAAGGTGTAACCCCTAAATCGATTTTCTCTTCACTTGATTTCAATCTCTATATTGTAGATCTTGAATCTTGATTGTGTTTTTTGATTTTGTAGAATTCTCCAAGGTTGCTGTTCGTACCGCGATTGGTTTCGTTGTGATGGGATTTGTTGGGTTTTTCGTTAAGCTTATCTTTATTCCAATCAACAACATCATCGTTGGATCTGGTTAG
Protein sequence	>MS.gene94163.t1 MDAIDSVFDPLREFAKDSVRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSG