Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013382.t1 | XP_003602486.1 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 8.70E-201 | 709.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013382.t1 | Q9SVA6 | 91.8 | 353 | 28 | 1 | 1 | 352 | 1 | 353 | 3.2e-188 | 659.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013382.t1 | G7JAA5 | 99.7 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 6.2e-201 | 709.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013382 | MS.gene30893 | PPI |
| MS.gene072150 | MS.gene013382 | PPI |
| MS.gene25152 | MS.gene013382 | PPI |
| MS.gene011134 | MS.gene013382 | PPI |
| MS.gene07894 | MS.gene013382 | PPI |
| MS.gene013382 | MS.gene46830 | PPI |
| MS.gene013382 | MS.gene36673 | PPI |
| MS.gene83732 | MS.gene013382 | PPI |
| MS.gene029882 | MS.gene013382 | PPI |
| MS.gene013382 | MS.gene36568 | PPI |
| MS.gene013382 | MS.gene30367 | PPI |
| MS.gene013382 | MS.gene74304 | PPI |
| MS.gene92847 | MS.gene013382 | PPI |
| MS.gene073797 | MS.gene013382 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013382.t1 | MTR_3g094880 | 99.716 | 352 | 1 | 0 | 1 | 352 | 1 | 352 | 0.0 | 725 |
| MS.gene013382.t1 | MTR_8g020210 | 57.143 | 350 | 147 | 2 | 4 | 351 | 3 | 351 | 3.19e-139 | 403 |
| MS.gene013382.t1 | MTR_2g019790 | 35.652 | 115 | 71 | 2 | 65 | 176 | 319 | 433 | 1.11e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013382.t1 | AT4G39520 | 91.218 | 353 | 30 | 1 | 1 | 352 | 1 | 353 | 0.0 | 658 |
| MS.gene013382.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene013382.t1 | AT1G17470 | 56.286 | 350 | 150 | 2 | 4 | 351 | 3 | 351 | 1.28e-144 | 417 |
| MS.gene013382.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene013382.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene013382.t1 | AT1G72660 | 55.143 | 350 | 154 | 2 | 4 | 351 | 3 | 351 | 1.30e-141 | 409 |
| MS.gene013382.t1 | AT1G72660 | 52.222 | 270 | 127 | 1 | 84 | 351 | 2 | 271 | 4.18e-103 | 308 |
| MS.gene013382.t1 | AT5G18570 | 27.513 | 189 | 114 | 4 | 65 | 230 | 379 | 567 | 3.00e-13 | 72.0 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATGCAGATATTACTTTA+AGG | 0.195713 | 3.4:+86317404 | MS.gene013382:CDS |
| ATAAGACGCTTATGAGTTAT+TGG | 0.217930 | 3.4:-86315915 | None:intergenic |
| TCCTCTTCCCTTTCCATCCT+TGG | 0.232626 | 3.4:-86315101 | None:intergenic |
| AGCCACCAAACCTGACTTTC+AGG | 0.267365 | 3.4:+86317284 | MS.gene013382:CDS |
| TTCTTAGATGACAATATTAC+AGG | 0.277031 | 3.4:-86318095 | None:intergenic |
| AATGATGCAGTGCTCTGGTC+TGG | 0.288648 | 3.4:+86318718 | MS.gene013382:intron |
| ATGGGCTTCTGGATAAAGTT+TGG | 0.291273 | 3.4:+86318009 | MS.gene013382:CDS |
| GTGGAACCTTGATGGGCTTC+TGG | 0.293542 | 3.4:+86317998 | MS.gene013382:CDS |
| AAGGCTACTGCTCATCATTT+GGG | 0.300257 | 3.4:+86312220 | MS.gene013382:CDS |
| CAAGGCTACTGCTCATCATT+TGG | 0.308308 | 3.4:+86312219 | MS.gene013382:CDS |
| TAGTCAGGATTCATACCTTT+AGG | 0.323820 | 3.4:-86318065 | None:intergenic |
| ACTACACTAACTTGCATTCC+AGG | 0.339401 | 3.4:+86314100 | MS.gene013382:CDS |
| TTCCTCCTGAAAGTCAGGTT+TGG | 0.374686 | 3.4:-86317289 | None:intergenic |
| TTTCTTTCCTCCTGAAAGTC+AGG | 0.381939 | 3.4:-86317294 | None:intergenic |
| CTCCTGAAAGTCAGGTTTGG+TGG | 0.386899 | 3.4:-86317286 | None:intergenic |
| ATGATGCAGTGCTCTGGTCT+GGG | 0.389872 | 3.4:+86318719 | MS.gene013382:intron |
| TGGAAAGGGAAGAGGAAGGC+AGG | 0.392906 | 3.4:+86315108 | MS.gene013382:CDS |
| ATTACAGGGTCTTCGTAGTC+AGG | 0.396058 | 3.4:-86318080 | None:intergenic |
| TGGGCTTCTGGATAAAGTTT+GGG | 0.396911 | 3.4:+86318010 | MS.gene013382:CDS |
| TCAGATCACACTTGAGGAAC+TGG | 0.403962 | 3.4:+86317513 | MS.gene013382:CDS |
| TTGGCACCCTCAATAATTCC+AGG | 0.407131 | 3.4:-86315082 | None:intergenic |
| TCACGGTATCAAGATCTAGA+TGG | 0.410734 | 3.4:-86317354 | None:intergenic |
| ACTCCTTACACCTACAACTA+AGG | 0.413818 | 3.4:+86312887 | MS.gene013382:CDS |
| CCGCCACCTCCCTTAGTTGT+AGG | 0.416550 | 3.4:-86312897 | None:intergenic |
| CACCTGGAGTGGAACCTTGA+TGG | 0.417281 | 3.4:+86317990 | MS.gene013382:CDS |
| ACTTGAGGAACTGGAGATCT+TGG | 0.417927 | 3.4:+86317522 | MS.gene013382:CDS |
| ACCTGGAGTGGAACCTTGAT+GGG | 0.417928 | 3.4:+86317991 | MS.gene013382:CDS |
| TGCCCTCAATGACATCTATA+AGG | 0.420928 | 3.4:-86317447 | None:intergenic |
| AGCTAGAAGGATTTGGCATA+AGG | 0.430372 | 3.4:+86315946 | MS.gene013382:CDS |
| CTCGCTACATATAGCCTTCA+CGG | 0.438245 | 3.4:-86317371 | None:intergenic |
| TTCCTCATTACTGCCCTGTC+AGG | 0.441209 | 3.4:+86317551 | MS.gene013382:CDS |
| CTCCCTTAGTTGTAGGTGTA+AGG | 0.443291 | 3.4:-86312890 | None:intergenic |
| TTCCAAATGATGCAGTGCTC+TGG | 0.443660 | 3.4:+86318713 | MS.gene013382:intron |
| GCCCATCAAGGTTCCACTCC+AGG | 0.455391 | 3.4:-86317992 | None:intergenic |
| GACTTTCAGGAGGAAAGAAA+AGG | 0.462402 | 3.4:+86317297 | MS.gene013382:CDS |
| ACTAAGGGAGGTGGCGGTGC+TGG | 0.471973 | 3.4:+86312903 | MS.gene013382:CDS |
| GATCTCCCTGGAATTATTGA+GGG | 0.477457 | 3.4:+86315076 | MS.gene013382:CDS |
| TGTCAGTTGCTGGATCTCCC+TGG | 0.485451 | 3.4:+86315064 | MS.gene013382:intron |
| CTTGTTCACAACATAGATGC+AGG | 0.490496 | 3.4:-86317485 | None:intergenic |
| CGTATTTATACTAAACCTAA+AGG | 0.494629 | 3.4:+86318050 | MS.gene013382:CDS |
| GGAGGTGGCGGTGCTGGTGA+AGG | 0.497652 | 3.4:+86312909 | MS.gene013382:CDS |
| GGATCTCCCTGGAATTATTG+AGG | 0.497825 | 3.4:+86315075 | MS.gene013382:CDS |
| ACTCTGCTGAATAAATTGAC+TGG | 0.498625 | 3.4:+86312999 | MS.gene013382:CDS |
| CTCGTGGGCTTCCCGTCAGT+TGG | 0.504184 | 3.4:+86312969 | MS.gene013382:CDS |
| CTCTGCTGAATAAATTGACT+GGG | 0.511261 | 3.4:+86313000 | MS.gene013382:CDS |
| AGTTAGTGTAGTGAACTCAT+AGG | 0.513041 | 3.4:-86314089 | None:intergenic |
| CATCCTCTAGTATCTCATGC+AGG | 0.523981 | 3.4:-86319003 | None:intergenic |
| ATTATTGAGGGTGCCAAGGA+TGG | 0.524161 | 3.4:+86315088 | MS.gene013382:CDS |
| GCCAAGGATGGAAAGGGAAG+AGG | 0.527508 | 3.4:+86315100 | MS.gene013382:CDS |
| CCACGATATGTGATCACACC+TGG | 0.532149 | 3.4:-86314118 | None:intergenic |
| CTTTATCCAGAAGCCCATCA+AGG | 0.533921 | 3.4:-86318004 | None:intergenic |
| TCTGTTATTCAGTGCACACC+TGG | 0.544525 | 3.4:+86317974 | MS.gene013382:intron |
| TGGAATTATTGAGGGTGCCA+AGG | 0.547326 | 3.4:+86315084 | MS.gene013382:CDS |
| TGAGGGTGCCAAGGATGGAA+AGG | 0.549980 | 3.4:+86315093 | MS.gene013382:CDS |
| CACGGTATCAAGATCTAGAT+GGG | 0.550347 | 3.4:-86317353 | None:intergenic |
| GATGCAAGAGTCGGTCTCGT+GGG | 0.558118 | 3.4:+86312954 | MS.gene013382:CDS |
| CTCCTTACACCTACAACTAA+GGG | 0.561218 | 3.4:+86312888 | MS.gene013382:CDS |
| GGATGCAAGAGTCGGTCTCG+TGG | 0.561453 | 3.4:+86312953 | MS.gene013382:CDS |
| GAGGGTGCCAAGGATGGAAA+GGG | 0.568429 | 3.4:+86315094 | MS.gene013382:CDS |
| GAGTAGACTTGCCAACTGAC+GGG | 0.569689 | 3.4:-86312980 | None:intergenic |
| GACCTTATAGATGTCATTGA+GGG | 0.569803 | 3.4:+86317445 | MS.gene013382:CDS |
| TCATCATTTGGGGTTACTGA+AGG | 0.570485 | 3.4:+86312231 | MS.gene013382:CDS |
| AGAGTAGACTTGCCAACTGA+CGG | 0.570529 | 3.4:-86312981 | None:intergenic |
| TGATGCAGTGCTCTGGTCTG+GGG | 0.575620 | 3.4:+86318720 | MS.gene013382:intron |
| CTAAACTTGCAAAACTGAGG+AGG | 0.585745 | 3.4:+86312862 | MS.gene013382:CDS |
| GGTTACAAGAATTACCTGAC+AGG | 0.586053 | 3.4:-86317565 | None:intergenic |
| ATCCTCTAGTATCTCATGCA+GGG | 0.588051 | 3.4:-86319002 | None:intergenic |
| TACCTGACAGGGCAGTAATG+AGG | 0.589669 | 3.4:-86317553 | None:intergenic |
| ACTTTCAGGAGGAAAGAAAA+GGG | 0.590692 | 3.4:+86317298 | MS.gene013382:CDS |
| TCAGGAGGAAAGAAAAGGGT+GGG | 0.592993 | 3.4:+86317302 | MS.gene013382:CDS |
| TGACCTTATAGATGTCATTG+AGG | 0.593679 | 3.4:+86317444 | MS.gene013382:CDS |
| AATCAAAGACATCGAAGATG+AGG | 0.596340 | 3.4:+86311768 | MS.gene013382:CDS |
| ATCTATAAGGTCATCTGCAG+TGG | 0.597376 | 3.4:-86317434 | None:intergenic |
| TATTCAGTGCACACCTGGAG+TGG | 0.599235 | 3.4:+86317979 | MS.gene013382:intron |
| AGTATCTCATGCAGGGAATG+TGG | 0.603443 | 3.4:-86318995 | None:intergenic |
| TTTCTGCGATCGCATTCACA+AGG | 0.607440 | 3.4:+86318133 | MS.gene013382:CDS |
| TAAACTTGCAAAACTGAGGA+GGG | 0.608244 | 3.4:+86312863 | MS.gene013382:CDS |
| AGGCTACTGCTCATCATTTG+GGG | 0.608492 | 3.4:+86312221 | MS.gene013382:CDS |
| TCGTTCTTCAACAATGGCGA+CGG | 0.609637 | 3.4:+86311735 | None:intergenic |
| TCTTAGATGACAATATTACA+GGG | 0.622650 | 3.4:-86318094 | None:intergenic |
| ACACCTACAACTAAGGGAGG+TGG | 0.623374 | 3.4:+86312894 | MS.gene013382:CDS |
| CAAGCCTCAGAGAGTCGGCA+AGG | 0.623563 | 3.4:+86318758 | MS.gene013382:CDS |
| AAACACAAGCCTCAGAGAGT+CGG | 0.626180 | 3.4:+86318753 | MS.gene013382:CDS |
| TCTCATGCAGGGAATGTGGT+CGG | 0.637938 | 3.4:-86318991 | None:intergenic |
| GATTGATCAGATCACACTTG+AGG | 0.641269 | 3.4:+86317507 | MS.gene013382:CDS |
| CCAGGTGTGATCACATATCG+TGG | 0.641756 | 3.4:+86314118 | MS.gene013382:CDS |
| TGGCACCCTCAATAATTCCA+GGG | 0.644735 | 3.4:-86315081 | None:intergenic |
| AAAAGTGGGGATGCAAGAGT+CGG | 0.647820 | 3.4:+86312945 | MS.gene013382:CDS |
| CACAGGTTATTAGTACTGCA+AGG | 0.648199 | 3.4:+86315853 | MS.gene013382:intron |
| TCTAGATCTTGATACCGTGA+AGG | 0.652544 | 3.4:+86317357 | MS.gene013382:CDS |
| TTCAGGAGGAAAGAAAAGGG+TGG | 0.654637 | 3.4:+86317301 | MS.gene013382:CDS |
| TCTACCTTGCCGACTCTCTG+AGG | 0.654923 | 3.4:-86318762 | None:intergenic |
| CCTACAACTAAGGGAGGTGG+CGG | 0.656986 | 3.4:+86312897 | MS.gene013382:CDS |
| AGGCTAAACTTGCAAAACTG+AGG | 0.657417 | 3.4:+86312859 | MS.gene013382:intron |
| TTGTTCACAACATAGATGCA+GGG | 0.658113 | 3.4:-86317484 | None:intergenic |
| TTCCCTGCATGAGATACTAG+AGG | 0.664860 | 3.4:+86319000 | MS.gene013382:CDS |
| GGCGAAAACTCAAAAGAACA+AGG | 0.674392 | 3.4:+86312201 | MS.gene013382:CDS |
| CACCAAACCTGACTTTCAGG+AGG | 0.677415 | 3.4:+86317287 | MS.gene013382:CDS |
| AGGATGGAAAGGGAAGAGGA+AGG | 0.692562 | 3.4:+86315104 | MS.gene013382:CDS |
| GTTACAAGAATTACCTGACA+GGG | 0.705478 | 3.4:-86317564 | None:intergenic |
| CTTACACCTACAACTAAGGG+AGG | 0.707070 | 3.4:+86312891 | MS.gene013382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 86311748 | 86319030 | 86311748 | ID=MS.gene013382 |
| chr3.4 | mRNA | 86311748 | 86319030 | 86311748 | ID=MS.gene013382.t1;Parent=MS.gene013382 |
| chr3.4 | exon | 86311748 | 86311789 | 86311748 | ID=MS.gene013382.t1.exon1;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86311748 | 86311789 | 86311748 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86312199 | 86312252 | 86312199 | ID=MS.gene013382.t1.exon2;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86312199 | 86312252 | 86312199 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86312861 | 86313034 | 86312861 | ID=MS.gene013382.t1.exon3;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86312861 | 86313034 | 86312861 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86314082 | 86314153 | 86314082 | ID=MS.gene013382.t1.exon4;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86314082 | 86314153 | 86314082 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86315070 | 86315129 | 86315070 | ID=MS.gene013382.t1.exon5;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86315070 | 86315129 | 86315070 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86315858 | 86315967 | 86315858 | ID=MS.gene013382.t1.exon6;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86315858 | 86315967 | 86315858 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86317273 | 86317572 | 86317273 | ID=MS.gene013382.t1.exon7;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86317273 | 86317572 | 86317273 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86317986 | 86318177 | 86317986 | ID=MS.gene013382.t1.exon8;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86317986 | 86318177 | 86317986 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86318728 | 86318817 | 86318728 | ID=MS.gene013382.t1.exon9;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86318728 | 86318817 | 86318728 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| chr3.4 | exon | 86318919 | 86319030 | 86318919 | ID=MS.gene013382.t1.exon10;Parent=MS.gene013382.t1 |
| chr3.4 | CDS | 86318919 | 86319030 | 86318919 | ID=cds.MS.gene013382.t1;Parent=MS.gene013382.t1 |
| Gene Sequence |
| Protein sequence |
