Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36673.t1 | XP_013444254.1 | 98.4 | 254 | 4 | 0 | 1 | 254 | 1 | 254 | 3.10E-119 | 438 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36673.t1 | Q9CQK7 | 35.0 | 203 | 113 | 4 | 1 | 198 | 1 | 189 | 1.1e-19 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36673.t1 | A0A072TM40 | 98.4 | 254 | 4 | 0 | 1 | 254 | 1 | 254 | 2.2e-119 | 438.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene36673 | 0.83084 | 2.34E-55 | -1.69E-46 |
| MS.gene050277 | MS.gene36673 | 0.826221 | 3.05E-54 | -1.69E-46 |
| MS.gene050317 | MS.gene36673 | 0.824353 | 8.45E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene36673 | 0.809672 | 1.69E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene36673 | 0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene051245 | MS.gene36673 | 0.83219 | 1.09E-55 | -1.69E-46 |
| MS.gene051746 | MS.gene36673 | 0.801945 | 7.09E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene36673 | 0.800836 | 1.20E-48 | -1.69E-46 |
| MS.gene051757 | MS.gene36673 | 0.808925 | 2.44E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene36673 | 0.814387 | 1.58E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene36673 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene053381 | MS.gene36673 | 0.829156 | 6.03E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene36673 | 0.802274 | 6.06E-49 | -1.69E-46 |
| MS.gene056432 | MS.gene36673 | 0.821379 | 4.17E-53 | -1.69E-46 |
| MS.gene056631 | MS.gene36673 | 0.805421 | 1.35E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene36673 | 0.838712 | 2.44E-57 | -1.69E-46 |
| MS.gene057094 | MS.gene36673 | 0.82325 | 1.53E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene36673 | 0.855099 | 7.92E-62 | -1.69E-46 |
| MS.gene057234 | MS.gene36673 | 0.859352 | 4.39E-63 | -1.69E-46 |
| MS.gene058054 | MS.gene36673 | 0.817459 | 3.26E-52 | -1.69E-46 |
| MS.gene058055 | MS.gene36673 | 0.846175 | 2.56E-59 | -1.69E-46 |
| MS.gene058238 | MS.gene36673 | 0.823985 | 1.03E-53 | -1.69E-46 |
| MS.gene058646 | MS.gene36673 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene058654 | MS.gene36673 | 0.813298 | 2.75E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene36673 | 0.844359 | 7.94E-59 | -1.69E-46 |
| MS.gene059249 | MS.gene36673 | 0.80378 | 2.96E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene36673 | 0.806935 | 6.46E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene36673 | 0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene36673 | 0.833055 | 6.64E-56 | -1.69E-46 |
| MS.gene060586 | MS.gene36673 | 0.806913 | 6.53E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene36673 | 0.821586 | 3.73E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028300 | MS.gene36673 | PPI |
| MS.gene31037 | MS.gene36673 | PPI |
| MS.gene013382 | MS.gene36673 | PPI |
| MS.gene049359 | MS.gene36673 | PPI |
| MS.gene023154 | MS.gene36673 | PPI |
| MS.gene36492 | MS.gene36673 | PPI |
| MS.gene35157 | MS.gene36673 | PPI |
| MS.gene06479 | MS.gene36673 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36673.t1 | MTR_8g015590 | 98.819 | 254 | 3 | 0 | 1 | 254 | 1 | 254 | 0.0 | 509 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36673.t1 | AT1G51730 | 70.040 | 247 | 72 | 2 | 1 | 246 | 1 | 246 | 4.43e-105 | 305 |
Find 45 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATATCGTCTGAAGATTTA+AGG | 0.204105 | 8.2:-77747500 | MS.gene36673:CDS |
| AGGCATTCTTACATCTTCTT+TGG | 0.306333 | 8.2:+77747026 | None:intergenic |
| TTTAATCTGGAAGCACCTAT+TGG | 0.387103 | 8.2:+77749245 | None:intergenic |
| TTCATGTTAGAAATACATTC+TGG | 0.390456 | 8.2:-77749288 | MS.gene36673:intron |
| AGCTGTTGTCACTGAAGATT+TGG | 0.411502 | 8.2:-77743157 | MS.gene36673:CDS |
| GTGGTTTAAGCACTTCCAAT+AGG | 0.412630 | 8.2:-77749260 | MS.gene36673:CDS |
| TTCAAAAGCGGAGGTTCGTC+TGG | 0.413109 | 8.2:+77747671 | None:intergenic |
| AGATTTGGATGAGGAGGATG+AGG | 0.441858 | 8.2:-77743142 | MS.gene36673:CDS |
| CCACAGGCATCTGAAAATCT+TGG | 0.452799 | 8.2:-77747147 | MS.gene36673:intron |
| AACAGTAACTGCTTCTCCAT+GGG | 0.457881 | 8.2:+77745979 | None:intergenic |
| AGAAATGGAGATTGAAGCAT+TGG | 0.464035 | 8.2:-77749428 | MS.gene36673:CDS |
| CTACGCGCAAGAACAAGAAA+TGG | 0.480177 | 8.2:-77749443 | MS.gene36673:CDS |
| CAACAGTAACTGCTTCTCCA+TGG | 0.482263 | 8.2:+77745978 | None:intergenic |
| CCAGATTAAAATCACTGCTC+AGG | 0.484543 | 8.2:-77749232 | MS.gene36673:intron |
| GAAATACATTCTGGTGAAAG+TGG | 0.495312 | 8.2:-77749279 | MS.gene36673:CDS |
| ACTATACAAAACAGTTTGCA+AGG | 0.496821 | 8.2:-77747522 | MS.gene36673:intron |
| TCCAACTAACTCACCATGAC+AGG | 0.510196 | 8.2:-77749568 | None:intergenic |
| GACGAGTCAAATACTAATCC+AGG | 0.514669 | 8.2:-77747910 | MS.gene36673:intron |
| GAAAGACAAACTTCAACAAG+AGG | 0.517470 | 8.2:-77747472 | MS.gene36673:intron |
| GCAATTGTACAGGAGGAAGA+TGG | 0.524264 | 8.2:-77747934 | MS.gene36673:intron |
| GATGATGATGAGGATTTCGA+AGG | 0.528804 | 8.2:-77743105 | MS.gene36673:intron |
| TGCAATTTAACTCGCTCGCA+TGG | 0.532396 | 8.2:+77742925 | None:intergenic |
| GGCAGACAGTGGTTTGAAAG+TGG | 0.536978 | 8.2:-77745445 | MS.gene36673:CDS |
| GAAGTAGCAAGCACAGACTC+TGG | 0.546961 | 8.2:+77745487 | None:intergenic |
| CACTGAAGATTTGGATGAGG+AGG | 0.557813 | 8.2:-77743148 | MS.gene36673:CDS |
| GACAGTGGTTTGAAAGTGGA+AGG | 0.561439 | 8.2:-77745441 | MS.gene36673:CDS |
| CATTGACTTTGATGATGATG+AGG | 0.578083 | 8.2:-77743115 | MS.gene36673:CDS |
| GTCTGTGCTTGCTACTTCGA+AGG | 0.579213 | 8.2:-77745482 | MS.gene36673:CDS |
| ATACTCATGAATGAATTCAA+AGG | 0.581196 | 8.2:-77749400 | MS.gene36673:intron |
| TGGTGATGCTGAAGCAGAAG+AGG | 0.584742 | 8.2:-77747052 | MS.gene36673:CDS |
| GAAATTTGGAATACCTGTCA+TGG | 0.587000 | 8.2:+77749555 | None:intergenic |
| GGCATCTGAAAATCTTGGCA+TGG | 0.589383 | 8.2:-77747142 | MS.gene36673:CDS |
| TCGAAGGAAAAGAAACTCAC+TGG | 0.592415 | 8.2:-77745466 | MS.gene36673:CDS |
| CGCTCGCATGGAAGAATGTG+TGG | 0.597052 | 8.2:+77742937 | None:intergenic |
| GTGGTTTGAAAGTGGAAGGA+TGG | 0.600163 | 8.2:-77745437 | MS.gene36673:intron |
| CTAAAGAGTGGTTGACTGAG+CGG | 0.616560 | 8.2:-77747092 | MS.gene36673:CDS |
| TTGTTAGCTCAGCTAAAGAG+TGG | 0.628644 | 8.2:-77747104 | MS.gene36673:CDS |
| CTGCTCACATTCAAAAGCGG+AGG | 0.633056 | 8.2:+77747662 | None:intergenic |
| GACCTGCTCACATTCAAAAG+CGG | 0.648261 | 8.2:+77747659 | None:intergenic |
| TGTCACTGAAGATTTGGATG+AGG | 0.649651 | 8.2:-77743151 | MS.gene36673:CDS |
| TCTTAGATAGTGGTACCCCA+TGG | 0.659025 | 8.2:-77745995 | MS.gene36673:intron |
| TGATGCTGAAGCAGAAGAGG+CGG | 0.674289 | 8.2:-77747049 | MS.gene36673:CDS |
| ACAGTAACTGCTTCTCCATG+GGG | 0.675643 | 8.2:+77745980 | None:intergenic |
| AGAAACTCACTGGCAGACAG+TGG | 0.676924 | 8.2:-77745456 | MS.gene36673:CDS |
| CGGTATTGTGAAGACTCAGA+TGG | 0.683666 | 8.2:-77747072 | MS.gene36673:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 77742931 | 77749575 | 77742931 | ID=MS.gene36673 |
| chr8.2 | mRNA | 77742931 | 77749575 | 77742931 | ID=MS.gene36673.t1;Parent=MS.gene36673 |
| chr8.2 | exon | 77749569 | 77749575 | 77749569 | ID=MS.gene36673.t1.exon1;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77749569 | 77749575 | 77749569 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77749401 | 77749466 | 77749401 | ID=MS.gene36673.t1.exon2;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77749401 | 77749466 | 77749401 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77749233 | 77749300 | 77749233 | ID=MS.gene36673.t1.exon3;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77749233 | 77749300 | 77749233 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77747911 | 77747944 | 77747911 | ID=MS.gene36673.t1.exon4;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77747911 | 77747944 | 77747911 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77747662 | 77747734 | 77747662 | ID=MS.gene36673.t1.exon5;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77747662 | 77747734 | 77747662 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77747473 | 77747530 | 77747473 | ID=MS.gene36673.t1.exon6;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77747473 | 77747530 | 77747473 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77747038 | 77747163 | 77747038 | ID=MS.gene36673.t1.exon7;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77747038 | 77747163 | 77747038 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77745920 | 77746011 | 77745920 | ID=MS.gene36673.t1.exon8;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77745920 | 77746011 | 77745920 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77745438 | 77745516 | 77745438 | ID=MS.gene36673.t1.exon9;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77745438 | 77745516 | 77745438 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77743106 | 77743187 | 77743106 | ID=MS.gene36673.t1.exon10;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77743106 | 77743187 | 77743106 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| chr8.2 | exon | 77742931 | 77743010 | 77742931 | ID=MS.gene36673.t1.exon11;Parent=MS.gene36673.t1 |
| chr8.2 | CDS | 77742931 | 77743010 | 77742931 | ID=cds.MS.gene36673.t1;Parent=MS.gene36673.t1 |
| Gene Sequence |
| Protein sequence |
