AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014858

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014858.t1 MTR_3g106690 97.297 333 9 0 1 333 59 391 0.0 679
MS.gene014858.t1 MTR_7g103260 58.258 333 136 2 1 333 68 397 1.09e-148 424
MS.gene014858.t1 MTR_7g103270 58.859 333 134 2 1 333 69 398 7.05e-144 412
MS.gene014858.t1 MTR_1g061760 57.485 334 138 3 1 333 154 484 9.48e-144 415
MS.gene014858.t1 MTR_3g080690 57.704 331 137 2 1 331 115 442 3.15e-142 409
MS.gene014858.t1 MTR_3g081240 58.006 331 136 2 1 331 117 444 3.19e-142 410
MS.gene014858.t1 MTR_3g097310 58.258 333 136 2 1 333 162 491 5.95e-140 405
MS.gene014858.t1 MTR_4g115530 54.142 338 148 3 1 333 55 390 5.89e-131 379
MS.gene014858.t1 MTR_4g115530 54.142 338 148 3 1 333 1 336 1.10e-130 377
MS.gene014858.t1 MTR_4g115540 54.438 338 147 3 1 333 289 624 3.37e-129 383
MS.gene014858.t1 MTR_4g115460 52.976 336 153 3 1 333 129 462 2.46e-124 365
MS.gene014858.t1 MTR_4g100730 47.619 336 168 5 1 330 200 533 1.41e-102 311
MS.gene014858.t1 MTR_3g110198 46.386 332 170 4 1 329 264 590 2.48e-97 300
MS.gene014858.t1 MTR_7g103150 45.482 332 173 5 1 329 155 481 3.79e-97 296
MS.gene014858.t1 MTR_3g110200 44.578 332 176 5 1 329 261 587 2.39e-96 297
MS.gene014858.t1 MTR_1g006600 45.783 332 172 5 1 329 257 583 2.19e-94 292
MS.gene014858.t1 MTR_8g102840 42.643 333 182 4 1 329 279 606 7.30e-91 283
MS.gene014858.t1 MTR_4g115540 54.032 248 107 3 1 243 289 534 4.43e-90 280
MS.gene014858.t1 MTR_1g090733 43.114 334 176 6 1 330 222 545 5.41e-89 277
MS.gene014858.t1 MTR_5g031480 39.706 340 182 8 1 332 90 414 1.42e-76 241
MS.gene014858.t1 MTR_5g049790 39.169 337 172 9 1 332 126 434 1.70e-72 231
MS.gene014858.t1 MTR_7g085840 39.706 340 181 11 1 331 1 325 1.60e-64 207
MS.gene014858.t1 MTR_8g102840 40.323 248 139 4 1 244 279 521 3.70e-58 196
MS.gene014858.t1 MTR_5g031480 39.062 256 133 8 1 248 90 330 6.20e-48 164
MS.gene014858.t1 MTR_5g031480 38.824 255 133 8 1 247 90 329 2.52e-47 163
MS.gene014858.t1 MTR_5g049790 38.967 213 110 7 1 208 126 323 5.11e-36 132
MS.gene014858.t1 MTR_5g049790 38.967 213 110 7 1 208 143 340 5.50e-36 133
MS.gene014858.t1 MTR_8g096140 28.041 296 193 6 45 324 93 384 5.47e-29 115
MS.gene014858.t1 MTR_8g096160 28.716 296 191 7 45 324 93 384 8.17e-29 115
MS.gene014858.t1 MTR_2g084245 28.000 300 192 7 45 324 131 426 5.32e-25 105
MS.gene014858.t1 MTR_4g057500 28.896 308 179 10 45 324 149 444 4.78e-24 102
MS.gene014858.t1 MTR_8g080490 25.079 315 206 12 27 324 97 398 1.13e-16 80.9
MS.gene014858.t1 MTR_1g029350 24.924 329 206 9 2 302 71 386 2.66e-16 79.7
MS.gene014858.t1 MTR_3g037720 25.773 291 198 8 45 324 187 470 2.70e-16 80.1
MS.gene014858.t1 MTR_6g027310 26.452 310 191 12 45 334 183 475 3.31e-15 76.6
MS.gene014858.t1 MTR_4g084200 25.949 316 180 13 45 333 160 448 5.42e-15 75.9
MS.gene014858.t1 MTR_3g085480 26.263 297 190 12 45 324 116 400 8.23e-14 72.4
MS.gene014858.t1 MTR_4g077920 24.762 315 193 9 49 333 111 411 1.38e-13 71.6
MS.gene014858.t1 MTR_4g084200 27.559 254 141 10 45 274 160 394 4.08e-13 70.1
MS.gene014858.t1 MTR_4g084200 27.559 254 141 10 45 274 160 394 4.50e-13 70.1
MS.gene014858.t1 MTR_4g077920 25.954 262 157 8 95 333 222 469 4.14e-12 67.4
MS.gene014858.t1 MTR_7g087100 24.055 291 202 7 45 324 188 470 8.11e-12 66.2
MS.gene014858.t1 MTR_4g077920 27.350 234 147 8 95 312 222 448 1.58e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014858.t1 AT5G25310 67.365 334 107 2 1 333 147 479 6.14e-169 479
MS.gene014858.t1 AT5G03795 57.784 334 138 2 1 334 186 516 5.73e-143 414
MS.gene014858.t1 AT3G07620 57.357 333 140 1 1 333 128 458 2.13e-136 395
MS.gene014858.t1 AT5G11130 51.796 334 158 2 1 333 1 332 1.30e-132 381
MS.gene014858.t1 AT5G11130 51.796 334 158 2 1 333 145 476 2.13e-131 383
MS.gene014858.t1 AT5G20260 52.695 334 156 2 1 333 1 333 6.25e-126 364
MS.gene014858.t1 AT5G20260 52.695 334 156 2 1 333 28 360 7.02e-126 365
MS.gene014858.t1 AT5G20260 52.695 334 156 2 1 333 133 465 3.19e-125 367
MS.gene014858.t1 AT3G42180 50.440 341 159 3 1 333 1 339 1.70e-122 355
MS.gene014858.t1 AT3G42180 50.440 341 159 3 1 333 130 468 1.27e-121 358
MS.gene014858.t1 AT5G33290 51.613 341 155 3 1 333 160 498 1.53e-121 359
MS.gene014858.t1 AT4G16745 49.254 335 164 3 1 330 196 529 1.81e-112 337
MS.gene014858.t1 AT5G19670 46.547 333 169 4 1 329 277 604 2.33e-102 313
MS.gene014858.t1 AT5G19670 46.547 333 169 4 1 329 277 604 2.33e-102 313
MS.gene014858.t1 AT4G16745 49.032 310 152 3 1 305 196 504 1.11e-101 311
MS.gene014858.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.34e-96 296
MS.gene014858.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.34e-96 296
MS.gene014858.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.34e-96 296
MS.gene014858.t1 AT5G25820 44.012 334 178 5 1 329 320 649 9.34e-91 284
MS.gene014858.t1 AT5G37000 41.246 337 180 6 1 329 222 548 3.99e-84 265
MS.gene014858.t1 AT5G11610 42.090 335 180 10 1 329 216 542 4.18e-80 254
MS.gene014858.t1 AT4G38040 40.000 340 181 8 1 332 100 424 2.78e-74 235
MS.gene014858.t1 AT4G38040 39.550 311 165 8 1 303 100 395 5.83e-63 206
MS.gene014858.t1 AT5G11610 39.834 241 132 9 1 236 216 448 1.47e-47 166
MS.gene014858.t1 AT1G27440 29.097 299 186 9 45 324 89 380 1.66e-28 114
MS.gene014858.t1 AT5G61840 28.523 298 189 8 45 324 92 383 5.77e-28 113
MS.gene014858.t1 AT5G22940 29.126 309 188 10 45 330 152 452 7.09e-27 110
MS.gene014858.t1 AT5G22940 29.126 309 188 10 45 330 181 481 8.96e-27 110
MS.gene014858.t1 AT2G28110 27.042 355 209 10 5 324 94 433 1.71e-26 109
MS.gene014858.t1 AT5G11610 41.739 115 64 2 1 113 216 329 6.93e-25 103
MS.gene014858.t1 AT1G21480 26.531 294 174 13 5 270 89 368 9.97e-16 77.8
MS.gene014858.t1 AT1G21480 24.405 336 193 13 5 302 89 401 1.22e-15 77.8
MS.gene014858.t1 AT1G74680 27.027 296 188 9 45 324 149 432 9.69e-15 75.1
MS.gene014858.t1 AT1G67410 25.079 315 210 11 27 324 101 406 1.17e-14 74.7
MS.gene014858.t1 AT2G35100 25.597 293 167 12 45 310 127 395 2.08e-14 74.3
MS.gene014858.t1 AT5G16890 28.175 252 164 8 95 333 228 475 7.95e-13 69.7
MS.gene014858.t1 AT3G45400 31.933 119 78 1 218 333 327 445 6.31e-12 66.6
MS.gene014858.t1 AT3G03650 24.735 283 186 9 45 312 193 463 6.13e-11 63.9

Find 99 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCATAGAGATGAATTTC+TGG 0.201182 3.3:-87520961 None:intergenic
TCAGAGACAAACTGCGTTAA+AGG 0.217419 3.3:-87520410 None:intergenic
GAACTTGGAGCTTAGCATTA+TGG 0.244455 3.3:-87521160 None:intergenic
CGGTATCTTGCCTTCTTTGC+CGG 0.249948 3.3:+87521033 MS.gene014858:CDS
TAAATTGATTCCACAATTCT+TGG 0.255386 3.3:-87521216 None:intergenic
AAACCCCATGTTCCATTTCA+TGG 0.279328 3.3:-87520280 None:intergenic
TTACCAGAAATTCATCTCTA+TGG 0.286433 3.3:+87520958 MS.gene014858:CDS
GAATAGTAGTCTATGCCTTT+AGG 0.293641 3.3:-87521138 None:intergenic
TCATGCTTGCTTGCCATGAT+TGG 0.306171 3.3:+87520495 MS.gene014858:CDS
TATCAATGAGGTACCCCTTC+TGG 0.307231 3.3:+87520447 MS.gene014858:CDS
GGTAAAGATACATCCTTTAG+AGG 0.312438 3.3:-87520940 None:intergenic
TTCCAGTGTTGGAGAAGAAT+TGG 0.325056 3.3:-87521078 None:intergenic
TGGAGAAGAATTGGGCGAAT+TGG 0.350998 3.3:-87521069 None:intergenic
GGTGGAAGTGAAAGGAGTTT+AGG 0.373477 3.3:-87520994 None:intergenic
GGAGAAGAATTGGGCGAATT+GGG 0.381140 3.3:-87521068 None:intergenic
AATAGTAGTCTATGCCTTTA+GGG 0.390854 3.3:-87521137 None:intergenic
AGAAGCTCAAGCAAGGAGTA+AGG 0.412812 3.3:+87521388 MS.gene014858:CDS
TACCGGCGTGGGGCATTGTC+AGG 0.414760 3.3:-87521015 None:intergenic
ATGAAATGGAACATGGGGTT+TGG 0.415076 3.3:+87520282 MS.gene014858:CDS
TCCAGTGTTGGAGAAGAATT+GGG 0.427997 3.3:-87521077 None:intergenic
AGCTTCTTATACTCACTCTC+TGG 0.429763 3.3:-87521372 None:intergenic
TGAAGGTTTCAATCCTCTAA+AGG 0.433578 3.3:+87520927 MS.gene014858:CDS
AAATTGATTCCACAATTCTT+GGG 0.433600 3.3:-87521215 None:intergenic
AAAGTAAACATGAGCTGCAT+TGG 0.434974 3.3:-87520324 None:intergenic
AGGAAGGTTCCTCCATGAAA+TGG 0.439602 3.3:+87520268 MS.gene014858:CDS
GCCCAATTCTTCTCCAACAC+TGG 0.442592 3.3:+87521076 MS.gene014858:CDS
CATGCTTGCTTGCCATGATT+GGG 0.450735 3.3:+87520496 MS.gene014858:CDS
ACAATAGGTAGGTCCCCATC+TGG 0.451801 3.3:-87520209 None:intergenic
TGTTCATGATGGCCCGTGTA+AGG 0.468004 3.3:+87520229 MS.gene014858:CDS
TGGATGTTTCAGATATTCCT+AGG 0.469967 3.3:+87521325 MS.gene014858:CDS
CACGGGCCATCATGAACAAT+AGG 0.471146 3.3:-87520224 None:intergenic
TTCTTTGCCGGTGGTATGCA+TGG 0.475267 3.3:+87521045 MS.gene014858:CDS
CCTTCAGTGATGTGCTGCAA+TGG 0.481513 3.3:+87521283 MS.gene014858:CDS
ATGGAGGAACCTTCCTTCGA+TGG 0.484390 3.3:-87520261 None:intergenic
ACTTGGACAAAGACAGAACT+TGG 0.485720 3.3:-87521175 None:intergenic
TGCAATGCCAACACTTCTGA+AGG 0.485933 3.3:+87520910 MS.gene014858:CDS
AAGTAAACATGAGCTGCATT+GGG 0.487410 3.3:-87520323 None:intergenic
TTGAACAAATTACCCAATCA+TGG 0.495105 3.3:-87520508 None:intergenic
ATGAGCTGCATTGGGATCAC+TGG 0.499158 3.3:-87520315 None:intergenic
ACCATCCATGTCACACTGAA+TGG 0.500488 3.3:-87520350 None:intergenic
CTTCAGTGATGTGCTGCAAT+GGG 0.500866 3.3:+87521284 MS.gene014858:CDS
GCAGGGTCCACATGCATCAA+AGG 0.511644 3.3:+87520849 MS.gene014858:intron
ATGCATACCACCGGCAAAGA+AGG 0.518456 3.3:-87521043 None:intergenic
GTCAATGAGTATCTCCCTAA+AGG 0.518478 3.3:+87521123 MS.gene014858:CDS
AAGTTCTGTCTTTGTCCAAG+TGG 0.519604 3.3:+87521177 MS.gene014858:CDS
GAAGCTCAAGCAAGGAGTAA+GGG 0.521382 3.3:+87521389 MS.gene014858:CDS
ATTGAAACCTTCAGAAGTGT+TGG 0.527913 3.3:-87520917 None:intergenic
CTAAGAATCTCTTTCAACCT+AGG 0.532971 3.3:-87521342 None:intergenic
TAAAGGAGTGACGTTGTAAG+AGG 0.533141 3.3:-87520393 None:intergenic
CTAGCCACTTCAAAACCACT+TGG 0.537494 3.3:-87521192 None:intergenic
TGTAAGAGGATGGTGTATAG+AGG 0.538083 3.3:-87520379 None:intergenic
GGAGTGACGTTGTAAGAGGA+TGG 0.541914 3.3:-87520389 None:intergenic
AAGGTGTATGTATACCCAGA+TGG 0.543645 3.3:+87520194 MS.gene014858:CDS
GGAGATGGAGAAGATATTCA+AGG 0.549467 3.3:+87520175 None:intergenic
CAGGGTCCACATGCATCAAA+GGG 0.550516 3.3:+87520850 MS.gene014858:intron
CCATTGCAGCACATCACTGA+AGG 0.558494 3.3:-87521283 None:intergenic
CATTGTCAGGTGGAAGTGAA+AGG 0.562267 3.3:-87521002 None:intergenic
TTCCTCCATGAAATGGAACA+TGG 0.562897 3.3:+87520275 MS.gene014858:CDS
CCCTTCTGGAACAGAACTCA+TGG 0.563069 3.3:+87520461 MS.gene014858:CDS
CGGCAAAGAAGGCAAGATAC+CGG 0.565879 3.3:-87521032 None:intergenic
AGGTGTATGTATACCCAGAT+GGG 0.567201 3.3:+87520195 MS.gene014858:CDS
TAATAATTTGATAGAATCAC+AGG 0.575809 3.3:-87521252 None:intergenic
AATTGGGCCATGCATACCAC+CGG 0.576027 3.3:-87521052 None:intergenic
GACCTACCTATTGTTCATGA+TGG 0.577284 3.3:+87520218 MS.gene014858:CDS
AGTGGCTAGCCCAAGAATTG+TGG 0.578684 3.3:+87521206 MS.gene014858:CDS
TCCTCCATGAAATGGAACAT+GGG 0.584529 3.3:+87520276 MS.gene014858:CDS
CCATGAGTTCTGTTCCAGAA+GGG 0.585895 3.3:-87520461 None:intergenic
ACCATGAGTTCTGTTCCAGA+AGG 0.586634 3.3:-87520462 None:intergenic
TTCTACCATTCAGTGTGACA+TGG 0.586958 3.3:+87520345 MS.gene014858:CDS
ATGGAGGTATTGTAGAGGAA+AGG 0.590050 3.3:-87520877 None:intergenic
CGGCGTGGGGCATTGTCAGG+TGG 0.590629 3.3:-87521012 None:intergenic
AGAAGGCAAGATACCGGCGT+GGG 0.592284 3.3:-87521026 None:intergenic
CCAGAAATTCATCTCTATGG+AGG 0.596875 3.3:+87520961 MS.gene014858:CDS
TATCTTGCCTTCTTTGCCGG+TGG 0.597223 3.3:+87521036 MS.gene014858:CDS
GAGTATAAGAAGCTCAAGCA+AGG 0.597781 3.3:+87521381 MS.gene014858:CDS
CAATAGGTAGGTCCCCATCT+GGG 0.602453 3.3:-87520208 None:intergenic
CATGAGTTCTGTTCCAGAAG+GGG 0.604621 3.3:-87520460 None:intergenic
ACCATTCAGTGTGACATGGA+TGG 0.616761 3.3:+87520349 MS.gene014858:CDS
CCTCCATGAAATGGAACATG+GGG 0.617032 3.3:+87520277 MS.gene014858:CDS
ACATATACTCCATCGAAGGA+AGG 0.623622 3.3:+87520252 MS.gene014858:CDS
CATGGCAAGCAAGCATGAAG+TGG 0.628450 3.3:-87520490 None:intergenic
TGTCTCTGATTATGTGAGAG+TGG 0.630472 3.3:+87520424 MS.gene014858:CDS
CACGAATGGAGGTATTGTAG+AGG 0.630731 3.3:-87520882 None:intergenic
ATGGAGTATATGTCCTTACA+CGG 0.631773 3.3:-87520242 None:intergenic
CATTGCAGCACATCACTGAA+GGG 0.633240 3.3:-87521282 None:intergenic
AGCAAGGAGTAAGGGCTGTG+AGG 0.634342 3.3:+87521397 MS.gene014858:CDS
CAGTGATGTGCTGCAATGGG+AGG 0.641289 3.3:+87521287 MS.gene014858:CDS
GGCATTGCACAAGACACGAA+TGG 0.644582 3.3:-87520896 None:intergenic
CACCTGACAATGCCCCACGC+CGG 0.655258 3.3:+87521013 MS.gene014858:CDS
GGATTGCCCTTTGATGCATG+TGG 0.662943 3.3:-87520856 None:intergenic
AAGGACATATACTCCATCGA+AGG 0.665739 3.3:+87520248 MS.gene014858:CDS
GAAGGCAAGATACCGGCGTG+GGG 0.675913 3.3:-87521025 None:intergenic
AAGAAGGCAAGATACCGGCG+TGG 0.688297 3.3:-87521027 None:intergenic
TGGAGTATATGTCCTTACAC+GGG 0.689264 3.3:-87520241 None:intergenic
CCCCATGTTCCATTTCATGG+AGG 0.689291 3.3:-87520277 None:intergenic
ATGTGAGAGTGGTATCAATG+AGG 0.692550 3.3:+87520435 MS.gene014858:CDS
GGCCATCATGAACAATAGGT+AGG 0.694574 3.3:-87520220 None:intergenic
GGTGTATGTATACCCAGATG+GGG 0.701214 3.3:+87520196 MS.gene014858:CDS
ATTGCACAAGACACGAATGG+AGG 0.708664 3.3:-87520893 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTTAAAGGAAACAAA+AGG - chr3.3:87520829-87520848 None:intergenic 15.0%
!! AACATACAAAAACTATAGAT+TGG - chr3.3:87520660-87520679 None:intergenic 20.0%
!! AAGTGAAACTATTCATAAAA+TGG - chr3.3:87520568-87520587 None:intergenic 20.0%
!! TAATAATTTGATAGAATCAC+AGG - chr3.3:87521255-87521274 None:intergenic 20.0%
! ATTATTTACTTGACCTAAAG+TGG + chr3.3:87520784-87520803 MS.gene014858:intron 25.0%
! GAAACTATTCATAAAATGGA+TGG - chr3.3:87520564-87520583 None:intergenic 25.0%
! GTTTCCTTTAAATATATGCA+GGG + chr3.3:87520832-87520851 MS.gene014858:intron 25.0%
! TGTTTCCTTTAAATATATGC+AGG + chr3.3:87520831-87520850 MS.gene014858:intron 25.0%
! TTACTGTACTAACAAAATGT+TGG - chr3.3:87520683-87520702 None:intergenic 25.0%
!! AAAACATCAAATCTTTTAGC+CGG - chr3.3:87521444-87521463 None:intergenic 25.0%
!! AAATTGATTCCACAATTCTT+GGG - chr3.3:87521218-87521237 None:intergenic 25.0%
!! TAAATTGATTCCACAATTCT+TGG - chr3.3:87521219-87521238 None:intergenic 25.0%
AAAAGGCTTGAAATTCACAT+TGG - chr3.3:87520812-87520831 None:intergenic 30.0%
AAAGGCTTGAAATTCACATT+GGG - chr3.3:87520811-87520830 None:intergenic 30.0%
AACTAAAATGTGGGTAAAGT+TGG - chr3.3:87520615-87520634 None:intergenic 30.0%
AATAGTAGTCTATGCCTTTA+GGG - chr3.3:87521140-87521159 None:intergenic 30.0%
ATAAATGTGAAGTGTGGAAA+AGG - chr3.3:87520537-87520556 None:intergenic 30.0%
TAAATGTGAAGTGTGGAAAA+GGG - chr3.3:87520536-87520555 None:intergenic 30.0%
TTACCAGAAATTCATCTCTA+TGG + chr3.3:87520958-87520977 MS.gene014858:CDS 30.0%
TTGAACAAATTACCCAATCA+TGG - chr3.3:87520511-87520530 None:intergenic 30.0%
! ACATGATTTTGCACTCAATA+TGG + chr3.3:87521466-87521485 MS.gene014858:CDS 30.0%
!! TTTAGTTCTCCCAGTTATTA+GGG + chr3.3:87520628-87520647 MS.gene014858:intron 30.0%
!!! TTTTAGTTCTCCCAGTTATT+AGG + chr3.3:87520627-87520646 MS.gene014858:intron 30.0%
AAAGTAAACATGAGCTGCAT+TGG - chr3.3:87520327-87520346 None:intergenic 35.0%
AACTGGGAGAACTAAAATGT+GGG - chr3.3:87520624-87520643 None:intergenic 35.0%
AAGTAAACATGAGCTGCATT+GGG - chr3.3:87520326-87520345 None:intergenic 35.0%
ATGGAGTATATGTCCTTACA+CGG - chr3.3:87520245-87520264 None:intergenic 35.0%
CTAAGAATCTCTTTCAACCT+AGG - chr3.3:87521345-87521364 None:intergenic 35.0%
GAATAGTAGTCTATGCCTTT+AGG - chr3.3:87521141-87521160 None:intergenic 35.0%
GGTAAAGATACATCCTTTAG+AGG - chr3.3:87520943-87520962 None:intergenic 35.0%
TAACTGGGAGAACTAAAATG+TGG - chr3.3:87520625-87520644 None:intergenic 35.0%
TGAAGGTTTCAATCCTCTAA+AGG + chr3.3:87520927-87520946 MS.gene014858:CDS 35.0%
TGGACCCTGCATATATTTAA+AGG - chr3.3:87520839-87520858 None:intergenic 35.0%
TGGATGTTTCAGATATTCCT+AGG + chr3.3:87521325-87521344 MS.gene014858:CDS 35.0%
! ACATTTTACTCTGAATCAGC+CGG + chr3.3:87521422-87521441 MS.gene014858:CDS 35.0%
! ATTGAAACCTTCAGAAGTGT+TGG - chr3.3:87520920-87520939 None:intergenic 35.0%
AAACCCCATGTTCCATTTCA+TGG - chr3.3:87520283-87520302 None:intergenic 40.0%
AAGGACATATACTCCATCGA+AGG + chr3.3:87520248-87520267 MS.gene014858:CDS 40.0%
AAGGTGTATGTATACCCAGA+TGG + chr3.3:87520194-87520213 MS.gene014858:CDS 40.0%
AAGTTCTGTCTTTGTCCAAG+TGG + chr3.3:87521177-87521196 MS.gene014858:CDS 40.0%
ACATATACTCCATCGAAGGA+AGG + chr3.3:87520252-87520271 MS.gene014858:CDS 40.0%
ACTTGGACAAAGACAGAACT+TGG - chr3.3:87521178-87521197 None:intergenic 40.0%
AGCTTCTTATACTCACTCTC+TGG - chr3.3:87521375-87521394 None:intergenic 40.0%
AGGTGTATGTATACCCAGAT+GGG + chr3.3:87520195-87520214 MS.gene014858:CDS 40.0%
ATGAAATGGAACATGGGGTT+TGG + chr3.3:87520282-87520301 MS.gene014858:CDS 40.0%
ATGTGAGAGTGGTATCAATG+AGG + chr3.3:87520435-87520454 MS.gene014858:CDS 40.0%
ATTCACATTGGGACCACTTT+AGG - chr3.3:87520800-87520819 None:intergenic 40.0%
CCAGAAATTCATCTCTATGG+AGG + chr3.3:87520961-87520980 MS.gene014858:CDS 40.0%
CCTCCATAGAGATGAATTTC+TGG - chr3.3:87520964-87520983 None:intergenic 40.0%
GAACTTGGAGCTTAGCATTA+TGG - chr3.3:87521163-87521182 None:intergenic 40.0%
GACCTACCTATTGTTCATGA+TGG + chr3.3:87520218-87520237 MS.gene014858:CDS 40.0%
GAGTATAAGAAGCTCAAGCA+AGG + chr3.3:87521381-87521400 MS.gene014858:CDS 40.0%
GGCACAATAAATGTGAAGTG+TGG - chr3.3:87520543-87520562 None:intergenic 40.0%
GTCAATGAGTATCTCCCTAA+AGG + chr3.3:87521123-87521142 MS.gene014858:CDS 40.0%
TAAAGGAGTGACGTTGTAAG+AGG - chr3.3:87520396-87520415 None:intergenic 40.0%
TCAGAGACAAACTGCGTTAA+AGG - chr3.3:87520413-87520432 None:intergenic 40.0%
TCCTCCATGAAATGGAACAT+GGG + chr3.3:87520276-87520295 MS.gene014858:CDS 40.0%
TCTCCCAGTTATTAGGGATT+TGG + chr3.3:87520634-87520653 MS.gene014858:intron 40.0%
TGGAGTATATGTCCTTACAC+GGG - chr3.3:87520244-87520263 None:intergenic 40.0%
TGGCCAAATCCCTAATAACT+GGG - chr3.3:87520640-87520659 None:intergenic 40.0%
TGTAAGAGGATGGTGTATAG+AGG - chr3.3:87520382-87520401 None:intergenic 40.0%
TGTCTCTGATTATGTGAGAG+TGG + chr3.3:87520424-87520443 MS.gene014858:CDS 40.0%
TTCCTCCATGAAATGGAACA+TGG + chr3.3:87520275-87520294 MS.gene014858:CDS 40.0%
TTCTACCATTCAGTGTGACA+TGG + chr3.3:87520345-87520364 MS.gene014858:CDS 40.0%
TTGGCCAAATCCCTAATAAC+TGG - chr3.3:87520641-87520660 None:intergenic 40.0%
! TCCAGTGTTGGAGAAGAATT+GGG - chr3.3:87521080-87521099 None:intergenic 40.0%
! TTCCAGTGTTGGAGAAGAAT+TGG - chr3.3:87521081-87521100 None:intergenic 40.0%
!! ATGGAGGTATTGTAGAGGAA+AGG - chr3.3:87520880-87520899 None:intergenic 40.0%
!! TGTCTCTGTTTTTCCAGTGT+TGG - chr3.3:87521092-87521111 None:intergenic 40.0%
!!! TTGTCCAAGTGGTTTTGAAG+TGG + chr3.3:87521188-87521207 MS.gene014858:CDS 40.0%
ACCATCCATGTCACACTGAA+TGG - chr3.3:87520353-87520372 None:intergenic 45.0%
ACCATGAGTTCTGTTCCAGA+AGG - chr3.3:87520465-87520484 None:intergenic 45.0%
ACCATTCAGTGTGACATGGA+TGG + chr3.3:87520349-87520368 MS.gene014858:CDS 45.0%
AGAAGCTCAAGCAAGGAGTA+AGG + chr3.3:87521388-87521407 MS.gene014858:CDS 45.0%
AGGAAGGTTCCTCCATGAAA+TGG + chr3.3:87520268-87520287 MS.gene014858:CDS 45.0%
ATTGCACAAGACACGAATGG+AGG - chr3.3:87520896-87520915 None:intergenic 45.0%
CATGAGTTCTGTTCCAGAAG+GGG - chr3.3:87520463-87520482 None:intergenic 45.0%
CATGCTTGCTTGCCATGATT+GGG + chr3.3:87520496-87520515 MS.gene014858:CDS 45.0%
CATTGCAGCACATCACTGAA+GGG - chr3.3:87521285-87521304 None:intergenic 45.0%
CATTGTCAGGTGGAAGTGAA+AGG - chr3.3:87521005-87521024 None:intergenic 45.0%
CCATGAGTTCTGTTCCAGAA+GGG - chr3.3:87520464-87520483 None:intergenic 45.0%
CCTCCATGAAATGGAACATG+GGG + chr3.3:87520277-87520296 MS.gene014858:CDS 45.0%
CTAGCCACTTCAAAACCACT+TGG - chr3.3:87521195-87521214 None:intergenic 45.0%
CTTCAGTGATGTGCTGCAAT+GGG + chr3.3:87521284-87521303 MS.gene014858:CDS 45.0%
GAAGCTCAAGCAAGGAGTAA+GGG + chr3.3:87521389-87521408 MS.gene014858:CDS 45.0%
GGAGAAGAATTGGGCGAATT+GGG - chr3.3:87521071-87521090 None:intergenic 45.0%
GGCCATCATGAACAATAGGT+AGG - chr3.3:87520223-87520242 None:intergenic 45.0%
GGTGGAAGTGAAAGGAGTTT+AGG - chr3.3:87520997-87521016 None:intergenic 45.0%
GGTGTATGTATACCCAGATG+GGG + chr3.3:87520196-87520215 MS.gene014858:CDS 45.0%
TCATGCTTGCTTGCCATGAT+TGG + chr3.3:87520495-87520514 MS.gene014858:CDS 45.0%
TGCAATGCCAACACTTCTGA+AGG + chr3.3:87520910-87520929 MS.gene014858:CDS 45.0%
TGGAGAAGAATTGGGCGAAT+TGG - chr3.3:87521072-87521091 None:intergenic 45.0%
! TATCAATGAGGTACCCCTTC+TGG + chr3.3:87520447-87520466 MS.gene014858:CDS 45.0%
!! CACGAATGGAGGTATTGTAG+AGG - chr3.3:87520885-87520904 None:intergenic 45.0%
AATTGGGCCATGCATACCAC+CGG - chr3.3:87521055-87521074 None:intergenic 50.0%
ACAATAGGTAGGTCCCCATC+TGG - chr3.3:87520212-87520231 None:intergenic 50.0%
ATGAGCTGCATTGGGATCAC+TGG - chr3.3:87520318-87520337 None:intergenic 50.0%
ATGCATACCACCGGCAAAGA+AGG - chr3.3:87521046-87521065 None:intergenic 50.0%
ATGGAGGAACCTTCCTTCGA+TGG - chr3.3:87520264-87520283 None:intergenic 50.0%
CAATAGGTAGGTCCCCATCT+GGG - chr3.3:87520211-87520230 None:intergenic 50.0%
CACGGGCCATCATGAACAAT+AGG - chr3.3:87520227-87520246 None:intergenic 50.0%
CAGGGTCCACATGCATCAAA+GGG + chr3.3:87520850-87520869 MS.gene014858:intron 50.0%
CATGGCAAGCAAGCATGAAG+TGG - chr3.3:87520493-87520512 None:intergenic 50.0%
CCATTGCAGCACATCACTGA+AGG - chr3.3:87521286-87521305 None:intergenic 50.0%
CCCCATGTTCCATTTCATGG+AGG - chr3.3:87520280-87520299 None:intergenic 50.0%
CCTTCAGTGATGTGCTGCAA+TGG + chr3.3:87521283-87521302 MS.gene014858:CDS 50.0%
CGGCAAAGAAGGCAAGATAC+CGG - chr3.3:87521035-87521054 None:intergenic 50.0%
CGGTATCTTGCCTTCTTTGC+CGG + chr3.3:87521033-87521052 MS.gene014858:CDS 50.0%
GCCCAATTCTTCTCCAACAC+TGG + chr3.3:87521076-87521095 MS.gene014858:CDS 50.0%
GGAGTGACGTTGTAAGAGGA+TGG - chr3.3:87520392-87520411 None:intergenic 50.0%
GGATTGCCCTTTGATGCATG+TGG - chr3.3:87520859-87520878 None:intergenic 50.0%
GGCATTGCACAAGACACGAA+TGG - chr3.3:87520899-87520918 None:intergenic 50.0%
TATCTTGCCTTCTTTGCCGG+TGG + chr3.3:87521036-87521055 MS.gene014858:CDS 50.0%
TGTTCATGATGGCCCGTGTA+AGG + chr3.3:87520229-87520248 MS.gene014858:CDS 50.0%
TTCTTTGCCGGTGGTATGCA+TGG + chr3.3:87521045-87521064 MS.gene014858:CDS 50.0%
! AGTGGCTAGCCCAAGAATTG+TGG + chr3.3:87521206-87521225 MS.gene014858:CDS 50.0%
!! CCCTTCTGGAACAGAACTCA+TGG + chr3.3:87520461-87520480 MS.gene014858:CDS 50.0%
!!! ATGGGAGGCATTTTCAGTGC+AGG + chr3.3:87521302-87521321 MS.gene014858:CDS 50.0%
AAGAAGGCAAGATACCGGCG+TGG - chr3.3:87521030-87521049 None:intergenic 55.0%
AGAAGGCAAGATACCGGCGT+GGG - chr3.3:87521029-87521048 None:intergenic 55.0%
AGCAAGGAGTAAGGGCTGTG+AGG + chr3.3:87521397-87521416 MS.gene014858:CDS 55.0%
CAGTGATGTGCTGCAATGGG+AGG + chr3.3:87521287-87521306 MS.gene014858:CDS 55.0%
GCAGGGTCCACATGCATCAA+AGG + chr3.3:87520849-87520868 MS.gene014858:intron 55.0%
!!! GGAGGCATTTTCAGTGCAGG+TGG + chr3.3:87521305-87521324 MS.gene014858:CDS 55.0%
GAAGGCAAGATACCGGCGTG+GGG - chr3.3:87521028-87521047 None:intergenic 60.0%
CACCTGACAATGCCCCACGC+CGG + chr3.3:87521013-87521032 MS.gene014858:CDS 65.0%
!! TACCGGCGTGGGGCATTGTC+AGG - chr3.3:87521018-87521037 None:intergenic 65.0%
!! CGGCGTGGGGCATTGTCAGG+TGG - chr3.3:87521015-87521034 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 87520179 87521518 87520179 ID=MS.gene014858
chr3.3 mRNA 87520179 87521518 87520179 ID=MS.gene014858.t1;Parent=MS.gene014858
chr3.3 exon 87520179 87520517 87520179 ID=MS.gene014858.t1.exon1;Parent=MS.gene014858.t1
chr3.3 CDS 87520179 87520517 87520179 ID=cds.MS.gene014858.t1;Parent=MS.gene014858.t1
chr3.3 exon 87520853 87521518 87520853 ID=MS.gene014858.t1.exon2;Parent=MS.gene014858.t1
chr3.3 CDS 87520853 87521518 87520853 ID=cds.MS.gene014858.t1;Parent=MS.gene014858.t1
Gene Sequence

>MS.gene014858

ATGGAGAAGATATTCAAGGTGTATGTATACCCAGATGGGGACCTACCTATTGTTCATGATGGCCCGTGTAAGGACATATACTCCATCGAAGGAAGGTTCCTCCATGAAATGGAACATGGGGTTTGGAAGTTTAGAACCAGTGATCCCAATGCAGCTCATGTTTACTTTCTACCATTCAGTGTGACATGGATGGTCAAATACCTCTATACACCATCCTCTTACAACGTCACTCCTTTAACGCAGTTTGTCTCTGATTATGTGAGAGTGGTATCAATGAGGTACCCCTTCTGGAACAGAACTCATGGTGCTGACCACTTCATGCTTGCTTGCCATGATTGGGTAATTTGTTCAATTCCCTTTTCCACACTTCACATTTATTGTGCCATCCATTTTATGAATAGTTTCACTTTTTGTTAATGACACACAATTGATGCCAACTTTACCCACATTTTAGTTCTCCCAGTTATTAGGGATTTGGCCAATCTATAGTTTTTGTATGTTCCAACATTTTGTTAGTACAGTAAAAAAATTAATAGTTGATATTGTAACATTTGTTATGCATATACATTTAATATCAATTATTGATTTATTTATCAAACTTTTATATTATTTACTTGACCTAAAGTGGTCCCAATGTGAATTTCAAGCCTTTTGTTTCCTTTAAATATATGCAGGGTCCACATGCATCAAAGGGCAATCCTTTCCTCTACAATACCTCCATTCGTGTCTTGTGCAATGCCAACACTTCTGAAGGTTTCAATCCTCTAAAGGATGTATCTTTACCAGAAATTCATCTCTATGGAGGTGAAGTATCTCCTAAACTCCTTTCACTTCCACCTGACAATGCCCCACGCCGGTATCTTGCCTTCTTTGCCGGTGGTATGCATGGCCCAATTCGCCCAATTCTTCTCCAACACTGGAAAAACAGAGACAAAGATATTATTGTCAATGAGTATCTCCCTAAAGGCATAGACTACTATTCCATAATGCTAAGCTCCAAGTTCTGTCTTTGTCCAAGTGGTTTTGAAGTGGCTAGCCCAAGAATTGTGGAATCAATTTATGCAGAATGTGTACCTGTGATTCTATCAAATTATTATGTGTTGCCCTTCAGTGATGTGCTGCAATGGGAGGCATTTTCAGTGCAGGTGGATGTTTCAGATATTCCTAGGTTGAAAGAGATTCTTAGTGCTATTCCAGAGAGTGAGTATAAGAAGCTCAAGCAAGGAGTAAGGGCTGTGAGGAGACATTTTACTCTGAATCAGCCGGCTAAAAGATTTGATGTTTTTCACATGATTTTGCACTCAATATGGCTAAGAAGATTGAATATAAAACTTGAATAG
Protein sequence

>MS.gene014858.t1

MEKIFKVYVYPDGDLPIVHDGPCKDIYSIEGRFLHEMEHGVWKFRTSDPNAAHVYFLPFSVTWMVKYLYTPSSYNVTPLTQFVSDYVRVVSMRYPFWNRTHGADHFMLACHDWGPHASKGNPFLYNTSIRVLCNANTSEGFNPLKDVSLPEIHLYGGEVSPKLLSLPPDNAPRRYLAFFAGGMHGPIRPILLQHWKNRDKDIIVNEYLPKGIDYYSIMLSSKFCLCPSGFEVASPRIVESIYAECVPVILSNYYVLPFSDVLQWEAFSVQVDVSDIPRLKEILSAIPESEYKKLKQGVRAVRRHFTLNQPAKRFDVFHMILHSIWLRRLNIKLE