AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015771

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015771.t1 MTR_8g103030 98.635 293 4 0 1 293 1 293 0.0 593
MS.gene015771.t1 MTR_6g488150 60.993 282 58 5 23 293 14 254 2.67e-108 315
MS.gene015771.t1 MTR_2g101500 54.627 335 101 11 1 293 1 326 2.49e-104 307
MS.gene015771.t1 MTR_5g030710 51.274 314 103 10 12 293 40 335 2.81e-95 285
MS.gene015771.t1 MTR_8g067530 50.482 311 107 9 12 293 64 356 1.08e-90 273
MS.gene015771.t1 MTR_5g067350 47.205 322 105 8 12 293 51 347 1.39e-82 253
MS.gene015771.t1 MTR_1g080860 48.214 280 92 8 24 292 15 252 1.90e-72 223
MS.gene015771.t1 MTR_1g093240 47.636 275 95 6 23 293 7 236 4.12e-70 217
MS.gene015771.t1 MTR_7g096090 63.125 160 55 2 135 293 94 250 2.87e-66 207
MS.gene015771.t1 MTR_1g080860 46.667 270 91 8 24 282 15 242 7.92e-65 204
MS.gene015771.t1 MTR_1g070520 62.424 165 51 4 135 292 84 244 1.08e-64 203
MS.gene015771.t1 MTR_1g093240 46.008 263 93 6 23 281 7 224 1.50e-61 195
MS.gene015771.t1 MTR_6g488150 52.709 203 45 5 23 214 14 176 4.12e-56 179
MS.gene015771.t1 MTR_1g093240 42.798 243 90 6 23 261 7 204 1.54e-49 163
MS.gene015771.t1 MTR_1g070830 45.385 260 70 11 24 281 12 201 8.91e-49 161
MS.gene015771.t1 MTR_1g076270 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene015771.t1 MTR_1g093350 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene015771.t1 MTR_1g040675 41.473 258 75 9 24 281 1 182 5.85e-45 150
MS.gene015771.t1 MTR_1g069495 43.169 183 71 3 139 288 81 263 1.73e-37 134
MS.gene015771.t1 MTR_5g067350 38.272 243 89 7 12 214 51 272 6.20e-35 127
MS.gene015771.t1 MTR_1g040675 38.235 238 71 8 24 261 1 162 8.42e-35 124
MS.gene015771.t1 MTR_2g100780 42.442 172 76 5 136 284 92 263 1.20e-32 121
MS.gene015771.t1 MTR_4g011880 49.333 150 45 4 138 285 52 172 2.19e-32 118
MS.gene015771.t1 MTR_2g100780 42.945 163 72 4 136 277 92 254 2.43e-32 120
MS.gene015771.t1 MTR_2g102490 53.636 110 38 3 170 276 84 183 2.48e-31 116
MS.gene015771.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 4.96e-29 112
MS.gene015771.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 5.33e-29 112
MS.gene015771.t1 MTR_3g106850 30.921 152 79 4 131 277 79 209 2.45e-19 84.7
MS.gene015771.t1 MTR_4g124300 35.811 148 63 5 135 276 61 182 5.12e-18 80.5
MS.gene015771.t1 MTR_7g110790 36.090 133 59 4 146 274 45 155 1.97e-16 75.5
MS.gene015771.t1 MTR_1g085750 37.607 117 57 2 165 281 76 176 2.82e-16 75.5
MS.gene015771.t1 MTR_4g115075 30.769 143 62 3 141 275 93 206 4.40e-14 70.1
MS.gene015771.t1 MTR_1g109510 28.571 161 85 4 114 274 38 168 9.88e-12 62.8
MS.gene015771.t1 MTR_1g085750 36.082 97 47 1 165 261 76 157 3.99e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015771.t1 AT4G29080 55.864 324 91 11 1 293 3 305 8.04e-102 300
MS.gene015771.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene015771.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene015771.t1 AT2G22670 48.993 298 97 7 24 292 64 335 3.19e-84 256
MS.gene015771.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene015771.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene015771.t1 AT5G65670 46.128 297 110 6 24 292 61 335 6.07e-74 230
MS.gene015771.t1 AT5G65670 45.819 299 110 7 24 292 61 337 4.98e-72 225
MS.gene015771.t1 AT4G14550 48.913 276 87 9 24 293 1 228 5.46e-70 216
MS.gene015771.t1 AT3G04730 53.778 225 76 5 93 292 14 235 1.91e-69 215
MS.gene015771.t1 AT3G23050 49.301 286 81 12 24 293 6 243 1.61e-68 213
MS.gene015771.t1 AT1G04250 45.956 272 100 6 21 292 4 228 1.51e-65 205
MS.gene015771.t1 AT4G14550 47.727 264 84 9 24 281 1 216 7.32e-63 198
MS.gene015771.t1 AT4G14550 44.444 243 81 9 24 260 1 195 1.22e-49 163
MS.gene015771.t1 AT3G23050 45.059 253 76 11 24 261 6 210 1.03e-48 161
MS.gene015771.t1 AT1G04240 41.313 259 75 10 24 282 5 186 9.95e-47 155
MS.gene015771.t1 AT1G04240 41.473 258 74 10 24 281 105 285 2.87e-45 155
MS.gene015771.t1 AT5G43700 52.667 150 47 6 133 282 57 182 2.44e-42 144
MS.gene015771.t1 AT3G23030 48.993 149 45 2 133 281 53 170 4.11e-39 135
MS.gene015771.t1 AT2G33310 44.068 177 69 4 133 287 69 237 6.25e-39 137
MS.gene015771.t1 AT3G15540 37.313 268 91 9 23 288 5 197 1.06e-38 135
MS.gene015771.t1 AT3G23030 48.993 149 45 2 133 281 140 257 1.79e-38 136
MS.gene015771.t1 AT1G04550 45.946 185 65 7 133 293 63 236 4.79e-38 134
MS.gene015771.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene015771.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene015771.t1 AT1G52830 36.726 226 100 6 56 281 2 184 1.04e-36 129
MS.gene015771.t1 AT4G14560 49.020 153 49 5 126 278 41 164 3.00e-35 125
MS.gene015771.t1 AT4G28640 44.910 167 70 4 133 282 81 242 7.41e-35 126
MS.gene015771.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.17e-34 126
MS.gene015771.t1 AT3G16500 40.964 166 75 4 130 277 92 252 2.24e-34 126
MS.gene015771.t1 AT3G16500 40.964 166 75 4 130 277 92 252 3.01e-34 125
MS.gene015771.t1 AT1G15580 44.366 142 49 2 135 276 48 159 4.69e-34 122
MS.gene015771.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.43e-33 124
MS.gene015771.t1 AT1G04100 41.916 167 68 4 142 281 95 259 6.05e-31 117
MS.gene015771.t1 AT5G25890 36.250 160 74 3 125 284 39 170 1.25e-29 110
MS.gene015771.t1 AT1G51950 42.683 164 67 6 136 277 92 250 3.91e-29 112
MS.gene015771.t1 AT4G14550 40.513 195 65 8 24 212 1 150 3.96e-28 107
MS.gene015771.t1 AT3G23050 41.429 210 60 11 24 218 6 167 1.84e-27 105
MS.gene015771.t1 AT3G17600 37.931 145 64 5 130 274 36 154 9.61e-23 92.4
MS.gene015771.t1 AT2G46990 39.423 104 47 1 173 276 85 172 7.18e-19 82.4
MS.gene015771.t1 AT3G62100 38.393 112 48 3 169 275 74 169 1.11e-18 82.0
MS.gene015771.t1 AT2G46990 40.449 89 37 1 173 261 85 157 2.51e-14 69.7
MS.gene015771.t1 AT3G62100 37.755 98 40 3 169 261 74 155 7.19e-14 68.2
MS.gene015771.t1 AT4G32280 35.294 102 45 2 173 274 118 198 4.25e-13 67.4
MS.gene015771.t1 AT4G32280 35.294 102 45 2 173 274 160 240 1.17e-12 67.0
MS.gene015771.t1 AT1G15050 31.579 133 74 2 147 279 35 150 5.89e-12 63.2
MS.gene015771.t1 AT1G15050 31.579 133 74 2 147 279 67 182 1.31e-11 62.8
MS.gene015771.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8
MS.gene015771.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8

Find 81 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACCAATGCTTCTCTCTT+TGG 0.185471 4.3:-2341332 None:intergenic
CTCTCTTTGGTTTCCATTGA+AGG 0.229858 4.3:-2341319 None:intergenic
TCACCACCACCTTTAGCTTT+AGG 0.284997 4.3:-2341559 None:intergenic
GCTATTACTGATGGAGGTTC+TGG 0.289483 4.3:+2341469 MS.gene015771:CDS
AGATCCACTTTCCTCAAGTA+TGG 0.291849 4.3:-2342817 None:intergenic
GGCCTCCAATCCGATCTTTC+CGG 0.299985 4.3:+2342701 MS.gene015771:CDS
GAGTCACCAGAGAGAGAAAA+TGG 0.324653 4.3:+2341397 MS.gene015771:CDS
GCCTCAGTTCAGTAGCTTTG+AGG 0.337674 4.3:-2341357 None:intergenic
CCAGGTCAATGTGGCTCATA+TGG 0.347339 4.3:+2343528 MS.gene015771:intron
GTGAAACCATTGAGTTCTTC+CGG 0.369117 4.3:-2342720 None:intergenic
ATGAAGAGTTCTGAAGCAAT+TGG 0.391710 4.3:+2343881 MS.gene015771:CDS
AGCATCAGAGAAACCCCTTT+TGG 0.393944 4.3:-2341449 None:intergenic
TTGTTATCATGCAAAGGCTT+TGG 0.395919 4.3:-2341646 None:intergenic
TTCTCTGATGCTATTACTGA+TGG 0.411709 4.3:+2341460 MS.gene015771:CDS
TGGTGGTGTGCTTAAGAGTT+TGG 0.414516 4.3:+2341417 MS.gene015771:CDS
TTATGGAGAGGTTCCTTCAA+TGG 0.424280 4.3:+2341306 MS.gene015771:CDS
GTGCCAGTGGAACAAGGTTA+TGG 0.429339 4.3:+2341289 MS.gene015771:CDS
CTGATGGAGGTTCTGGGAAG+TGG 0.429765 4.3:+2341476 MS.gene015771:CDS
CTCTCTGGTGACTCTGAGCC+TGG 0.432328 4.3:-2341388 None:intergenic
CCGAGTCTTGCAAGAGGTTG+AGG 0.432404 4.3:+2343855 MS.gene015771:CDS
ATCACCATCCTTGTCTTCAT+AGG 0.451715 4.3:-2343625 None:intergenic
ACAACAACTGAAGCAGAGAA+AGG 0.456026 4.3:-2341601 None:intergenic
AGATTGGTGGATCTCCTTCA+TGG 0.457760 4.3:+2343585 MS.gene015771:CDS
CGAATCAGGGCGCAAGTTGT+GGG 0.462809 4.3:+2342676 MS.gene015771:intron
CTATTACTGATGGAGGTTCT+GGG 0.466403 4.3:+2341470 MS.gene015771:CDS
TGCTAGTTGGTGATGTTCCA+TGG 0.467311 4.3:+2343652 MS.gene015771:CDS
TTTGGTGTCTGGTGCCAAAA+GGG 0.469379 4.3:+2341435 MS.gene015771:CDS
TACATATATGCATTCAGACT+TGG 0.476242 4.3:-2342773 None:intergenic
TGGAAATGGTGGATCTGAAG+TGG 0.482511 4.3:+2341507 MS.gene015771:CDS
GCGAATCAGGGCGCAAGTTG+TGG 0.486260 4.3:+2342675 MS.gene015771:intron
GTGCTTAAGAGTTTGGTGTC+TGG 0.488896 4.3:+2341424 MS.gene015771:CDS
TGAAGAGTTCTGAAGCAATT+GGG 0.489200 4.3:+2343882 MS.gene015771:CDS
GTTTGGTGTCTGGTGCCAAA+AGG 0.490914 4.3:+2341434 MS.gene015771:CDS
GAACTGAGGCTAGGGTTACC+AGG 0.498587 4.3:+2341370 MS.gene015771:CDS
AAATCTAAGTGAAAGTAGAT+TGG 0.505003 4.3:+2343569 MS.gene015771:CDS
TTGTTCTCTCCTAAAGCTAA+AGG 0.505328 4.3:+2341550 MS.gene015771:CDS
TGATGGAGGTTCTGGGAAGT+GGG 0.506160 4.3:+2341477 MS.gene015771:CDS
GAAACCAAAGAGAGAAGCAT+TGG 0.506436 4.3:+2341328 MS.gene015771:CDS
ATCTTTCCGGAAGAACTCAA+TGG 0.508429 4.3:+2342714 MS.gene015771:CDS
TTGAGTTCTTCCGGAAAGAT+CGG 0.510793 4.3:-2342711 None:intergenic
ATGAAGACAAGGATGGTGAT+TGG 0.522716 4.3:+2343628 MS.gene015771:CDS
CAACAACTGAAGCAGAGAAA+GGG 0.523720 4.3:-2341600 None:intergenic
GGAAATGGTGGATCTGAAGT+GGG 0.526580 4.3:+2341508 MS.gene015771:CDS
TCTGATGCTATTACTGATGG+AGG 0.531086 4.3:+2341463 MS.gene015771:CDS
TTCCGGAAAGATCGGATTGG+AGG 0.531139 4.3:-2342703 None:intergenic
TCCTGCAGCACCTCGAGCCA+TGG 0.531399 4.3:+2343987 MS.gene015771:intron
AAGAAATCTTACTTTGCAGA+AGG 0.533764 4.3:-2341685 None:intergenic
TTGAGGAAAGTGGATCTGAA+TGG 0.538432 4.3:+2342823 MS.gene015771:CDS
TATGTAAAAGTCAGCATGGA+AGG 0.544619 4.3:+2342790 MS.gene015771:CDS
AAATCTTACTTTGCAGAAGG+AGG 0.546311 4.3:-2341682 None:intergenic
TCTAAGTGAAAGTAGATTGG+TGG 0.547921 4.3:+2343572 MS.gene015771:CDS
TCTCCATAACCTTGTTCCAC+TGG 0.551400 4.3:-2341292 None:intergenic
AAAGCTACTGAACTGAGGCT+AGG 0.551933 4.3:+2341361 MS.gene015771:CDS
AGTTCTGAAGCAATTGGGCT+AGG 0.552508 4.3:+2343887 MS.gene015771:CDS
TGCTCCATACTTGAGGAAAG+TGG 0.552637 4.3:+2342813 MS.gene015771:CDS
AAGCTACTGAACTGAGGCTA+GGG 0.557212 4.3:+2341362 MS.gene015771:CDS
TGTGGTTTGTTATCATGCAA+AGG 0.558132 4.3:-2341652 None:intergenic
GAAGTGGGGTTGTGTAAAGA+TGG 0.559129 4.3:+2341523 MS.gene015771:CDS
TTCTTCCGGAAAGATCGGAT+TGG 0.561734 4.3:-2342706 None:intergenic
TTGGTGTCTGGTGCCAAAAG+GGG 0.565847 4.3:+2341436 MS.gene015771:CDS
AGAAAATAGACGTACTCCCA+TGG 0.566126 4.3:-2343669 None:intergenic
CATATATGTAAAAGTCAGCA+TGG 0.572072 4.3:+2342786 MS.gene015771:CDS
GCTAGTTGGTGATGTTCCAT+GGG 0.572324 4.3:+2343653 MS.gene015771:CDS
TCTCCTAAAGCTAAAGGTGG+TGG 0.583056 4.3:+2341556 MS.gene015771:CDS
CCTCAACCTCTTGCAAGACT+CGG 0.585897 4.3:-2343855 None:intergenic
TTACATTTCTCCATGGCTCG+AGG 0.588053 4.3:-2343997 None:intergenic
TCAGGGCGCAAGTTGTGGGA+TGG 0.592117 4.3:+2342680 MS.gene015771:intron
GCACATATTCTGAACCATGA+AGG 0.604244 4.3:-2343599 None:intergenic
GATGGTGATTGGATGCTAGT+TGG 0.610602 4.3:+2343639 MS.gene015771:CDS
TGGAAGGTGCTCCATACTTG+AGG 0.612400 4.3:+2342806 MS.gene015771:CDS
GCAGAAGGAGGAGAAACATG+TGG 0.623815 4.3:-2341670 None:intergenic
ATGTCTGTGCCAGTGGAACA+AGG 0.641481 4.3:+2341283 MS.gene015771:CDS
TTCTCTCCTAAAGCTAAAGG+TGG 0.652446 4.3:+2341553 MS.gene015771:CDS
TTGACAAATCCAGGTCAATG+TGG 0.662027 4.3:+2343519 MS.gene015771:intron
TGTGCTTACCTATGAAGACA+AGG 0.670147 4.3:+2343617 MS.gene015771:CDS
TGTTCACCGAGTCTTGCAAG+AGG 0.674237 4.3:+2343849 MS.gene015771:CDS
GAAATGGTGGATCTGAAGTG+GGG 0.680430 4.3:+2341509 MS.gene015771:CDS
CTTACCTATGAAGACAAGGA+TGG 0.683773 4.3:+2343621 MS.gene015771:CDS
AGTGGAACAAGGTTATGGAG+AGG 0.703860 4.3:+2341294 MS.gene015771:CDS
TCACCAGAGAGAGAAAATGG+TGG 0.713194 4.3:+2341400 MS.gene015771:CDS
ACCTCAAAGCTACTGAACTG+AGG 0.714271 4.3:+2341356 MS.gene015771:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTTAAGTTTAACATT+TGG - chr4.3:2343169-2343188 None:intergenic 15.0%
!!! GATGTTATTTTTGTTATTTT+AGG + chr4.3:2343825-2343844 MS.gene015771:intron 15.0%
!!! GATTTTAATATAACAAACAT+TGG - chr4.3:2343918-2343937 None:intergenic 15.0%
!!! TCATTTTATTTAAGCAAAAA+AGG - chr4.3:2343482-2343501 None:intergenic 15.0%
!! AAGTAAGATTTCTTCTTATT+TGG + chr4.3:2341694-2341713 MS.gene015771:intron 20.0%
!!! ACTTGTTGTATCTTTTATTA+TGG - chr4.3:2342066-2342085 None:intergenic 20.0%
!!! ATTGATAGTTAGACTTATTT+TGG + chr4.3:2343748-2343767 MS.gene015771:intron 20.0%
!!! TGATTTCTTTTTTCTCAATA+CGG + chr4.3:2342645-2342664 MS.gene015771:intron 20.0%
! AAATCTAAGTGAAAGTAGAT+TGG + chr4.3:2343569-2343588 MS.gene015771:CDS 25.0%
! AACATGCAGAAGAAAATTTA+AGG - chr4.3:2342399-2342418 None:intergenic 25.0%
! AGATTTATATTATCATGCGT+TGG - chr4.3:2342575-2342594 None:intergenic 25.0%
! CGATCATAAATTCAAAACTA+AGG - chr4.3:2342612-2342631 None:intergenic 25.0%
! GCAAAAATATTTGAAAATCG+AGG + chr4.3:2343058-2343077 MS.gene015771:intron 25.0%
! TGACATGAATTTATGAACAT+CGG + chr4.3:2343243-2343262 MS.gene015771:intron 25.0%
! TTGATTCTAACATTGTTCAT+AGG - chr4.3:2343774-2343793 None:intergenic 25.0%
!! TCTATTGTTGGTTGAAATTA+TGG + chr4.3:2343337-2343356 MS.gene015771:intron 25.0%
!!! ACTTTTTTCACAAGCTATTA+TGG + chr4.3:2342121-2342140 MS.gene015771:intron 25.0%
!!! AGTTTTGAATTTATGATCGT+TGG + chr4.3:2342613-2342632 MS.gene015771:intron 25.0%
!!! GATACCTTCATATTTTTTGT+CGG + chr4.3:2343946-2343965 MS.gene015771:intron 25.0%
!!! TTCACATAGATAGTTACTTT+TGG + chr4.3:2342368-2342387 MS.gene015771:intron 25.0%
AAGAAATCTTACTTTGCAGA+AGG - chr4.3:2341688-2341707 None:intergenic 30.0%
AGATATTTGTGATCATGCAT+CGG - chr4.3:2342971-2342990 None:intergenic 30.0%
CATATATGTAAAAGTCAGCA+TGG + chr4.3:2342786-2342805 MS.gene015771:CDS 30.0%
GATTCCGACAAAAAATATGA+AGG - chr4.3:2343953-2343972 None:intergenic 30.0%
TACATATATGCATTCAGACT+TGG - chr4.3:2342776-2342795 None:intergenic 30.0%
TATGTGCTCTAGTAAATTTC+AGG + chr4.3:2342319-2342338 MS.gene015771:intron 30.0%
TCAAATAAGCCAATCCAAAT+AGG + chr4.3:2342223-2342242 MS.gene015771:intron 30.0%
TCAACCAACAATAGAGAAAA+TGG - chr4.3:2343332-2343351 None:intergenic 30.0%
TGCATGTATTTATATGTCAC+AGG + chr4.3:2343383-2343402 MS.gene015771:intron 30.0%
! GTTGAATCTGATGTTTCTTT+TGG + chr4.3:2341890-2341909 MS.gene015771:intron 30.0%
! TATATTCTTATGTTAGCCTC+TGG + chr4.3:2342266-2342285 MS.gene015771:intron 30.0%
! TCAACCATTTTCTCTATTGT+TGG + chr4.3:2343325-2343344 MS.gene015771:intron 30.0%
!! ATGTTTTTGTTGACAAATCC+AGG + chr4.3:2343510-2343529 MS.gene015771:intron 30.0%
!!! AATCACTTTTGAGACTGTTT+GGG - chr4.3:2342187-2342206 None:intergenic 30.0%
!!! GAATGGTTTTAGCTCTTATA+GGG + chr4.3:2342840-2342859 MS.gene015771:CDS 30.0%
!!! TAATCACTTTTGAGACTGTT+TGG - chr4.3:2342188-2342207 None:intergenic 30.0%
!!! TGAATGGTTTTAGCTCTTAT+AGG + chr4.3:2342839-2342858 MS.gene015771:CDS 30.0%
AAATCTTACTTTGCAGAAGG+AGG - chr4.3:2341685-2341704 None:intergenic 35.0%
ACATCAAGACACACAGATAA+TGG - chr4.3:2342943-2342962 None:intergenic 35.0%
ATAATTAGCAACAACCACCA+AGG - chr4.3:2342249-2342268 None:intergenic 35.0%
ATCTTCATTCTCAACCATGT+TGG - chr4.3:2343430-2343449 None:intergenic 35.0%
CATCAAGACACACAGATAAT+GGG - chr4.3:2342942-2342961 None:intergenic 35.0%
GCATGATCACAAATATCTCT+CGG + chr4.3:2342973-2342992 MS.gene015771:intron 35.0%
TATGTAAAAGTCAGCATGGA+AGG + chr4.3:2342790-2342809 MS.gene015771:CDS 35.0%
TCTAAGTGAAAGTAGATTGG+TGG + chr4.3:2343572-2343591 MS.gene015771:CDS 35.0%
TTCTCTGATGCTATTACTGA+TGG + chr4.3:2341460-2341479 MS.gene015771:CDS 35.0%
TTGTTATCATGCAAAGGCTT+TGG - chr4.3:2341649-2341668 None:intergenic 35.0%
TTGTTCTCTCCTAAAGCTAA+AGG + chr4.3:2341550-2341569 MS.gene015771:CDS 35.0%
! ATACGGTTTTTATGCGAATC+AGG + chr4.3:2342662-2342681 MS.gene015771:intron 35.0%
! ATGGTTGAAAGAGAGTGTAT+TGG - chr4.3:2343313-2343332 None:intergenic 35.0%
! CAAAGGTATGACTACTGTAT+AGG - chr4.3:2343455-2343474 None:intergenic 35.0%
! GAAAGAGAGTGTATTGGTAA+CGG - chr4.3:2343307-2343326 None:intergenic 35.0%
! TAAGCAAAAAAGGCATGCAA+AGG - chr4.3:2343472-2343491 None:intergenic 35.0%
! TACGGTTTTTATGCGAATCA+GGG + chr4.3:2342663-2342682 MS.gene015771:intron 35.0%
! TGTGCTTTGTTGATTGATGT+TGG + chr4.3:2341755-2341774 MS.gene015771:intron 35.0%
!! ATGAAGAGTTCTGAAGCAAT+TGG + chr4.3:2343881-2343900 MS.gene015771:CDS 35.0%
!! TGAAGAGTTCTGAAGCAATT+GGG + chr4.3:2343882-2343901 MS.gene015771:CDS 35.0%
!! TGTGGTTTGTTATCATGCAA+AGG - chr4.3:2341655-2341674 None:intergenic 35.0%
AAATCGAGGTGTGTCTTGAA+TGG + chr4.3:2343072-2343091 MS.gene015771:intron 40.0%
AAGACATGGAAAAGGCTAAC+TGG - chr4.3:2342505-2342524 None:intergenic 40.0%
AATATCTCTCGGTGATGTGA+TGG + chr4.3:2342984-2343003 MS.gene015771:intron 40.0%
ACAACAACTGAAGCAGAGAA+AGG - chr4.3:2341604-2341623 None:intergenic 40.0%
ACTGGACAAAATCAAATCCC+AGG - chr4.3:2342487-2342506 None:intergenic 40.0%
AGAAAATAGACGTACTCCCA+TGG - chr4.3:2343672-2343691 None:intergenic 40.0%
AGATCCACTTTCCTCAAGTA+TGG - chr4.3:2342820-2342839 None:intergenic 40.0%
AGCATCAGAAGACATGGAAA+AGG - chr4.3:2342513-2342532 None:intergenic 40.0%
ATCACCATCCTTGTCTTCAT+AGG - chr4.3:2343628-2343647 None:intergenic 40.0%
ATCTTTCCGGAAGAACTCAA+TGG + chr4.3:2342714-2342733 MS.gene015771:CDS 40.0%
ATGAAGACAAGGATGGTGAT+TGG + chr4.3:2343628-2343647 MS.gene015771:CDS 40.0%
CAAACCAATGCTTCTCTCTT+TGG - chr4.3:2341335-2341354 None:intergenic 40.0%
CAACAACTGAAGCAGAGAAA+GGG - chr4.3:2341603-2341622 None:intergenic 40.0%
CAACTGTACTGTCGAAGTAT+AGG - chr4.3:2343219-2343238 None:intergenic 40.0%
CTATTACTGATGGAGGTTCT+GGG + chr4.3:2341470-2341489 MS.gene015771:CDS 40.0%
CTGGACAAAATCAAATCCCA+GGG - chr4.3:2342486-2342505 None:intergenic 40.0%
CTTACCTATGAAGACAAGGA+TGG + chr4.3:2343621-2343640 MS.gene015771:CDS 40.0%
GCACATATTCTGAACCATGA+AGG - chr4.3:2343602-2343621 None:intergenic 40.0%
GTGAAACCATTGAGTTCTTC+CGG - chr4.3:2342723-2342742 None:intergenic 40.0%
TCTGATGCTATTACTGATGG+AGG + chr4.3:2341463-2341482 MS.gene015771:CDS 40.0%
TGTGCTTACCTATGAAGACA+AGG + chr4.3:2343617-2343636 MS.gene015771:CDS 40.0%
TTATGGAGAGGTTCCTTCAA+TGG + chr4.3:2341306-2341325 MS.gene015771:CDS 40.0%
TTCTCTCCTAAAGCTAAAGG+TGG + chr4.3:2341553-2341572 MS.gene015771:CDS 40.0%
TTGACAAATCCAGGTCAATG+TGG + chr4.3:2343519-2343538 MS.gene015771:intron 40.0%
TTGAGGAAAGTGGATCTGAA+TGG + chr4.3:2342823-2342842 MS.gene015771:CDS 40.0%
TTGAGTTCTTCCGGAAAGAT+CGG - chr4.3:2342714-2342733 None:intergenic 40.0%
! CTCTCTTTGGTTTCCATTGA+AGG - chr4.3:2341322-2341341 None:intergenic 40.0%
! GAAACCAAAGAGAGAAGCAT+TGG + chr4.3:2341328-2341347 MS.gene015771:CDS 40.0%
! GCGACTTTTACATTTCTCCA+TGG - chr4.3:2344007-2344026 None:intergenic 40.0%
! TATGTCACAGGTTCTGATTG+TGG + chr4.3:2343395-2343414 MS.gene015771:intron 40.0%
!! AAGCAGAGAAAGGGTTTTGT+TGG - chr4.3:2341594-2341613 None:intergenic 40.0%
!! AAGTGGGTTTTGTCTGGAAA+TGG + chr4.3:2341493-2341512 MS.gene015771:CDS 40.0%
AAAGCTACTGAACTGAGGCT+AGG + chr4.3:2341361-2341380 MS.gene015771:CDS 45.0%
AAATCCCAGGGCATACACAA+GGG - chr4.3:2342474-2342493 None:intergenic 45.0%
AACAACCACCAAGGCCTATT+TGG - chr4.3:2342240-2342259 None:intergenic 45.0%
AAGCCAATCCAAATAGGCCT+TGG + chr4.3:2342229-2342248 MS.gene015771:intron 45.0%
AAGCTACTGAACTGAGGCTA+GGG + chr4.3:2341362-2341381 MS.gene015771:CDS 45.0%
ACCTCAAAGCTACTGAACTG+AGG + chr4.3:2341356-2341375 MS.gene015771:CDS 45.0%
AGTGGAACAAGGTTATGGAG+AGG + chr4.3:2341294-2341313 MS.gene015771:CDS 45.0%
CCATGTCTTCTGATGCTGAA+AGG + chr4.3:2342516-2342535 MS.gene015771:intron 45.0%
CCTTTCAGCATCAGAAGACA+TGG - chr4.3:2342519-2342538 None:intergenic 45.0%
GAAGTGGGGTTGTGTAAAGA+TGG + chr4.3:2341523-2341542 MS.gene015771:CDS 45.0%
GAGTCACCAGAGAGAGAAAA+TGG + chr4.3:2341397-2341416 MS.gene015771:CDS 45.0%
GATGGTGATTGGATGCTAGT+TGG + chr4.3:2343639-2343658 MS.gene015771:CDS 45.0%
GCTATTACTGATGGAGGTTC+TGG + chr4.3:2341469-2341488 MS.gene015771:CDS 45.0%
TCACCAGAGAGAGAAAATGG+TGG + chr4.3:2341400-2341419 MS.gene015771:CDS 45.0%
TCTCCATAACCTTGTTCCAC+TGG - chr4.3:2341295-2341314 None:intergenic 45.0%
TCTCCTAAAGCTAAAGGTGG+TGG + chr4.3:2341556-2341575 MS.gene015771:CDS 45.0%
TGCTCCATACTTGAGGAAAG+TGG + chr4.3:2342813-2342832 MS.gene015771:CDS 45.0%
TTACATTTCTCCATGGCTCG+AGG - chr4.3:2344000-2344019 None:intergenic 45.0%
TTAGCCTCTGGTGTAAAAGC+TGG + chr4.3:2342278-2342297 MS.gene015771:intron 45.0%
TTCTCAACCATGTTGGCACT+TGG - chr4.3:2343423-2343442 None:intergenic 45.0%
TTCTTCCGGAAAGATCGGAT+TGG - chr4.3:2342709-2342728 None:intergenic 45.0%
! ACACCACCATTTTCTCTCTC+TGG - chr4.3:2341406-2341425 None:intergenic 45.0%
! AGATTGGTGGATCTCCTTCA+TGG + chr4.3:2343585-2343604 MS.gene015771:CDS 45.0%
! AGCATCAGAGAAACCCCTTT+TGG - chr4.3:2341452-2341471 None:intergenic 45.0%
! GCATCAGCATCGTTCTTTTG+AGG - chr4.3:2342748-2342767 None:intergenic 45.0%
! TGGTGGTGTGCTTAAGAGTT+TGG + chr4.3:2341417-2341436 MS.gene015771:CDS 45.0%
!! ACTCACTTGTTGATTCGCAC+TGG + chr4.3:2341962-2341981 MS.gene015771:intron 45.0%
!! ACTTGTTGATTCGCACTGGT+TGG + chr4.3:2341966-2341985 MS.gene015771:intron 45.0%
!! AGTTCTGAAGCAATTGGGCT+AGG + chr4.3:2343887-2343906 MS.gene015771:CDS 45.0%
!! GAAATGGTGGATCTGAAGTG+GGG + chr4.3:2341509-2341528 MS.gene015771:CDS 45.0%
!! GCTAGTTGGTGATGTTCCAT+GGG + chr4.3:2343653-2343672 MS.gene015771:CDS 45.0%
!! GGAAATGGTGGATCTGAAGT+GGG + chr4.3:2341508-2341527 MS.gene015771:CDS 45.0%
!! GTGCTTAAGAGTTTGGTGTC+TGG + chr4.3:2341424-2341443 MS.gene015771:CDS 45.0%
!! TCACCACCACCTTTAGCTTT+AGG - chr4.3:2341562-2341581 None:intergenic 45.0%
!! TGCTAGTTGGTGATGTTCCA+TGG + chr4.3:2343652-2343671 MS.gene015771:CDS 45.0%
!! TGGAAATGGTGGATCTGAAG+TGG + chr4.3:2341507-2341526 MS.gene015771:CDS 45.0%
!! TGGGTTTTGTCTGGAAATGG+TGG + chr4.3:2341496-2341515 MS.gene015771:CDS 45.0%
!! TTTGGTGTCTGGTGCCAAAA+GGG + chr4.3:2341435-2341454 MS.gene015771:CDS 45.0%
ATGTCTGTGCCAGTGGAACA+AGG + chr4.3:2341283-2341302 MS.gene015771:CDS 50.0%
CAAATCCCAGGGCATACACA+AGG - chr4.3:2342475-2342494 None:intergenic 50.0%
CCAATCCAAATAGGCCTTGG+TGG + chr4.3:2342232-2342251 MS.gene015771:intron 50.0%
CCACCAAGGCCTATTTGGAT+TGG - chr4.3:2342235-2342254 None:intergenic 50.0%
CCAGGTCAATGTGGCTCATA+TGG + chr4.3:2343528-2343547 MS.gene015771:intron 50.0%
CCATATGAGCCACATTGACC+TGG - chr4.3:2343531-2343550 None:intergenic 50.0%
CCTCAACCTCTTGCAAGACT+CGG - chr4.3:2343858-2343877 None:intergenic 50.0%
GCAGAAGGAGGAGAAACATG+TGG - chr4.3:2341673-2341692 None:intergenic 50.0%
GTGCCAGTGGAACAAGGTTA+TGG + chr4.3:2341289-2341308 MS.gene015771:CDS 50.0%
TGTTCACCGAGTCTTGCAAG+AGG + chr4.3:2343849-2343868 MS.gene015771:CDS 50.0%
! AACGCCAGCTTTTACACCAG+AGG - chr4.3:2342285-2342304 None:intergenic 50.0%
! GCCTCAGTTCAGTAGCTTTG+AGG - chr4.3:2341360-2341379 None:intergenic 50.0%
! TTCCGGAAAGATCGGATTGG+AGG - chr4.3:2342706-2342725 None:intergenic 50.0%
! TTGGTGTCTGGTGCCAAAAG+GGG + chr4.3:2341436-2341455 MS.gene015771:CDS 50.0%
!! GTTTGGTGTCTGGTGCCAAA+AGG + chr4.3:2341434-2341453 MS.gene015771:CDS 50.0%
!! TCTGGGAAGTGGGTTTTGTC+TGG + chr4.3:2341487-2341506 MS.gene015771:CDS 50.0%
!! TGATGGAGGTTCTGGGAAGT+GGG + chr4.3:2341477-2341496 MS.gene015771:CDS 50.0%
!! TGGAAGGTGCTCCATACTTG+AGG + chr4.3:2342806-2342825 MS.gene015771:CDS 50.0%
CCGAGTCTTGCAAGAGGTTG+AGG + chr4.3:2343855-2343874 MS.gene015771:CDS 55.0%
CGAATCAGGGCGCAAGTTGT+GGG + chr4.3:2342676-2342695 MS.gene015771:intron 55.0%
GAACTGAGGCTAGGGTTACC+AGG + chr4.3:2341370-2341389 MS.gene015771:CDS 55.0%
GGCCTCCAATCCGATCTTTC+CGG + chr4.3:2342701-2342720 MS.gene015771:CDS 55.0%
TGTGCCCTTGTGTATGCCCT+GGG + chr4.3:2342467-2342486 MS.gene015771:intron 55.0%
TTGTGCCCTTGTGTATGCCC+TGG + chr4.3:2342466-2342485 MS.gene015771:intron 55.0%
! TGTGGTGCCAAGTGCCAACA+TGG + chr4.3:2343413-2343432 MS.gene015771:intron 55.0%
!! CTGATGGAGGTTCTGGGAAG+TGG + chr4.3:2341476-2341495 MS.gene015771:CDS 55.0%
CTCTCTGGTGACTCTGAGCC+TGG - chr4.3:2341391-2341410 None:intergenic 60.0%
GCGAATCAGGGCGCAAGTTG+TGG + chr4.3:2342675-2342694 MS.gene015771:intron 60.0%
TCAGGGCGCAAGTTGTGGGA+TGG + chr4.3:2342680-2342699 MS.gene015771:intron 60.0%
TCCTGCAGCACCTCGAGCCA+TGG + chr4.3:2343987-2344006 MS.gene015771:intron 65.0%
!! TCCATGGCTCGAGGTGCTGC+AGG - chr4.3:2343991-2344010 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 2341283 2344032 2341283 ID=MS.gene015771
chr4.3 mRNA 2341283 2344032 2341283 ID=MS.gene015771.t1;Parent=MS.gene015771
chr4.3 exon 2341283 2341695 2341283 ID=MS.gene015771.t1.exon1;Parent=MS.gene015771.t1
chr4.3 CDS 2341283 2341695 2341283 ID=cds.MS.gene015771.t1;Parent=MS.gene015771.t1
chr4.3 exon 2342684 2342907 2342684 ID=MS.gene015771.t1.exon2;Parent=MS.gene015771.t1
chr4.3 CDS 2342684 2342907 2342684 ID=cds.MS.gene015771.t1;Parent=MS.gene015771.t1
chr4.3 exon 2343532 2343676 2343532 ID=MS.gene015771.t1.exon3;Parent=MS.gene015771.t1
chr4.3 CDS 2343532 2343676 2343532 ID=cds.MS.gene015771.t1;Parent=MS.gene015771.t1
chr4.3 exon 2343847 2343908 2343847 ID=MS.gene015771.t1.exon4;Parent=MS.gene015771.t1
chr4.3 CDS 2343847 2343908 2343847 ID=cds.MS.gene015771.t1;Parent=MS.gene015771.t1
chr4.3 exon 2343995 2344032 2343995 ID=MS.gene015771.t1.exon5;Parent=MS.gene015771.t1
chr4.3 CDS 2343995 2344032 2343995 ID=cds.MS.gene015771.t1;Parent=MS.gene015771.t1
Gene Sequence

>MS.gene015771

ATGTCTGTGCCAGTGGAACAAGGTTATGGAGAGGTTCCTTCAATGGAAACCAAAGAGAGAAGCATTGGTTTGAACCTCAAAGCTACTGAACTGAGGCTAGGGTTACCAGGCTCAGAGTCACCAGAGAGAGAAAATGGTGGTGTGCTTAAGAGTTTGGTGTCTGGTGCCAAAAGGGGTTTCTCTGATGCTATTACTGATGGAGGTTCTGGGAAGTGGGTTTTGTCTGGAAATGGTGGATCTGAAGTGGGGTTGTGTAAAGATGGAAACTTGTTCTCTCCTAAAGCTAAAGGTGGTGGTGAATGTAATAACCAACAAAACCCTTTCTCTGCTTCAGTTGTTGTTAAAGAAACTGTTACACACTCTCCAAAGCCTTTGCATGATAACAAACCACATGTTTCTCCTCCTTCTGCAAAGTAAGATTTCTTCTTATTTGGTTGTATGTTATGTTTCTCTTAGTGTTTGTGTATGTTGATGTGCTTTGTTGATTGATGTTGGCTCTTTGTTCATTGAAATATTTTTTGTTTATTTGTCTTGTCAGTTATCACTATTTAGTGTTTATAGTTTCTTCTGTGAGTTTACAAAATGTTGAAAACAAGAACAAAATTATGTTGAATCTGATGTTTCTTTTGGAAATTTGAACAGAAGTCAACTAACTCACTTTTTTTTTTTACAAGAACAAACTCACTTGTTGATTCGCACTGGTTGGATAAACAACTTAATTAAGTGCTTATAGCATTAGCACTTATCTTATATCTTATAAGTGCTTATATATAGCTATTTCCATAATAAAAGATACAACAAGTTCAAAGTGTTTTCATTTAAGCAATTTAAGCTATAAACTTTTTTCACAAGCTATTATGGAGAGCTTATGCTCATGTCATAAGTTGTATCTATAAGCTCTCCCAAACAGTCTCAAAAGTGATTATACTAGTAGATAAGCTCAAATAAGCCAATCCAAATAGGCCTTGGTGGTTGTTGCTAATTATATTCTTATGTTAGCCTCTGGTGTAAAAGCTGGCGTTGTTACTTGTAGTTGTATGTGCTCTAGTAAATTTCAGGATTTTCTATGTGCTAATCTTCAAAATTTCACATAGATAGTTACTTTTGGTTATTCCTTAAATTTTCTTCTGCATGTTGCATTCAATATCTTAAAAGTTTAAAATTTTATCTTAAAAGTTTTTTCTTGTGCCCTTGTGTATGCCCTGGGATTTGATTTTGTCCAGTTAGCCTTTTCCATGTCTTCTGATGCTGAAAGGTTTTTACTCAGCTTCTGTATTCATTGATTTTCACCAACGCATGATAATATAAATCTGTTTTAGCTTGATTCCTTAGTTTTGAATTTATGATCGTTGGCTTTAAAATTGATTTCTTTTTTCTCAATACGGTTTTTATGCGAATCAGGGCGCAAGTTGTGGGATGGCCTCCAATCCGATCTTTCCGGAAGAACTCAATGGTTTCACAGCCTCAAAAGAACGATGCTGATGCAGAAGCCAAGTCTGAATGCATATATGTAAAAGTCAGCATGGAAGGTGCTCCATACTTGAGGAAAGTGGATCTGAATGGTTTTAGCTCTTATAGGGAACTTTCTTCAGCACTCGAAAAGATGTTCAGTTGTTTTACAATCAGTGAGTTTTTATCTACTTGTTATCGTCTTCTCCCATTATCTGTGTGTCTTGATGTTTTCACCGATGCATGATCACAAATATCTCTCGGTGATGTGATGGTGATCATTCTAGTAAATGTATTGTACTTTGACGAACATTTTAGTTAAATGAGCAAAAATATTTGAAAATCGAGGTGTGTCTTGAATGGCTCAATAAATTCTTTACTAGAAAAAGTTCTTTATATAATTAATTGCATATGAAAAAGAAAACGTAATGAAACCAAATGTTAAACTTAAATAGTTAGCTCAAACACACAGACACACATTTTGCCTATACTTCGACAGTACAGTTGTTGATGACATGAATTTATGAACATCGGTCAATGTCAGTGTTGTATAGTGTTTCAATTGAAGAGAACCGTTACCAATACACTCTCTTTCAACCATTTTCTCTATTGTTGGTTGAAATTATGGCATATAAAAACTATTATAGAATATGCATGTATTTATATGTCACAGGTTCTGATTGTGGTGCCAAGTGCCAACATGGTTGAGAATGAAGATTACCTATACAGTAGTCATACCTTTGCATGCCTTTTTTGCTTAAATAAAATGATTCTGATGATGTTTTTGTTGACAAATCCAGGTCAATGTGGCTCATATGGAGTTTCTTGTCGAGAAAAAAATCTAAGTGAAAGTAGATTGGTGGATCTCCTTCATGGTTCAGAATATGTGCTTACCTATGAAGACAAGGATGGTGATTGGATGCTAGTTGGTGATGTTCCATGGGAGTACGTCTATTTTCTTATATATAATTCATAATTCACTACATGAATTAACAATGATTAGCTATGTTTGCTGAATTGATAGTTAGACTTATTTTGGCCTATGAACAATGTTAGAATCAACAAAGAGTGATTTAATTCTGATATCTGAAATGATGTTATTTTTGTTATTTTAGGATGTTCACCGAGTCTTGCAAGAGGTTGAGGATAATGAAGAGTTCTGAAGCAATTGGGCTAGGTAAGACCAATGTTTGTTATATTAAAATCTCATGTATGATACCTTCATATTTTTTGTCGGAATCATGTATACTCTTATTCCTGCAGCACCTCGAGCCATGGAGAAATGTAAAAGTCGCAACTAG
Protein sequence

>MS.gene015771.t1

MSVPVEQGYGEVPSMETKERSIGLNLKATELRLGLPGSESPERENGGVLKSLVSGAKRGFSDAITDGGSGKWVLSGNGGSEVGLCKDGNLFSPKAKGGGECNNQQNPFSASVVVKETVTHSPKPLHDNKPHVSPPSAKAQVVGWPPIRSFRKNSMVSQPQKNDADAEAKSECIYVKVSMEGAPYLRKVDLNGFSSYRELSSALEKMFSCFTISQCGSYGVSCREKNLSESRLVDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCKRLRIMKSSEAIGLAPRAMEKCKSRN