AlfalfaGEDB | MS.gene036277

M. sativa cultivar XinJiangDaYe / MS.gene036277

KEGG: K14484
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF02309.19

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036277.t1	XP_003612933.1	96.3	323	12	0	1	323	1	323	6.40E-178	633.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036277.t1	Q38826	65.9	311	74	7	21	323	21	307	3.4e-103	376.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036277.t1	G7KF56	96.3	323	12	0	1	323	1	323	4.6e-178	633.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene036277.t1	TR	AUX/IAA

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene015771	MS.gene036277	PPI
MS.gene04738	MS.gene036277	PPI
MS.gene049100	MS.gene036277	PPI
MS.gene05894	MS.gene036277	PPI
MS.gene061505	MS.gene036277	PPI
MS.gene68989	MS.gene036277	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036277.t1	MTR_5g030710	96.285	323	12	0	1	323	1	323	0.0	651
MS.gene036277.t1	MTR_8g067530	71.098	346	67	6	1	323	9	344	7.72e-173	483
MS.gene036277.t1	MTR_5g067350	59.494	316	102	11	21	323	33	335	1.97e-112	330
MS.gene036277.t1	MTR_2g101500	50.588	340	110	10	16	323	1	314	1.48e-99	296
MS.gene036277.t1	MTR_6g488150	54.380	274	78	6	51	323	15	242	3.54e-90	270
MS.gene036277.t1	MTR_8g103030	50.000	298	107	8	40	323	12	281	1.91e-87	264
MS.gene036277.t1	MTR_5g067350	53.200	250	94	10	21	257	33	272	2.50e-66	209
MS.gene036277.t1	MTR_1g080860	46.570	277	94	9	51	323	15	241	1.15e-64	204
MS.gene036277.t1	MTR_1g080860	46.570	277	94	9	51	323	15	241	1.37e-64	204
MS.gene036277.t1	MTR_1g093240	45.620	274	91	6	51	323	8	224	1.31e-63	201

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036277.t1	AT2G22670	67.203	311	70	9	21	323	21	307	9.18e-135	385
MS.gene036277.t1	AT2G22670	67.203	311	70	9	21	323	21	307	9.18e-135	385
MS.gene036277.t1	AT2G22670	67.203	311	70	9	21	323	21	307	1.05e-134	385
MS.gene036277.t1	AT2G22670	67.203	311	70	9	21	323	21	307	1.05e-134	385
MS.gene036277.t1	AT2G22670	67.203	311	70	9	21	323	38	324	1.57e-134	385
MS.gene036277.t1	AT5G65670	59.177	316	108	6	13	323	25	324	6.39e-124	358
MS.gene036277.t1	AT5G65670	59.177	316	108	6	13	323	25	324	7.77e-124	358
MS.gene036277.t1	AT4G29080	47.678	323	122	8	16	323	3	293	1.74e-87	265
MS.gene036277.t1	AT4G14550	45.421	273	92	5	51	323	1	216	5.84e-68	212
MS.gene036277.t1	AT4G14550	46.154	273	90	6	51	323	1	216	7.96e-68	212

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Find 98 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGAACTTGAATTTGA+AGG	0.413246	5.1:-20949380	MS.gene036277:CDS
AACAACAATGATGAAGTGGA+TGG	0.601893	5.1:-20948691	MS.gene036277:CDS
AACAGCTGAACATCTTCTCA+AGG	0.549658	5.1:+20948560	None:intergenic
AACTGTCGTCGACGATGGAA+CGG	0.600872	5.1:-20949477	MS.gene036277:CDS
AAGGCTACTGAGTTGAGGTT+GGG	0.419598	5.1:-20949361	MS.gene036277:CDS
ACAAAGAGTGCAGTGGGACC+TGG	0.505055	5.1:+20948664	None:intergenic
ACAGCTGAACATCTTCTCAA+GGG	0.651538	5.1:+20948561	None:intergenic
ACAGGGCACAAGTTGTTGGT+TGG	0.575157	5.1:-20948762	MS.gene036277:intron
ACCCGAGAGGGAAATGGATT+CGG	0.455743	5.1:-20949320	MS.gene036277:CDS
ACCTTCACAAAGAGTGCAGT+GGG	0.538673	5.1:+20948658	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTACCCCTAATAACTAT+TGG	-	chr5.1:20947635-20947654	MS.gene036277:intron	30.0%
AACACACCAATAGTTATTAG+GGG	+	chr5.1:20947644-20947663	None:intergenic	30.0%
AATAAGTCAACCCAGAAATA+TGG	+	chr5.1:20947896-20947915	None:intergenic	30.0%
ACCAATTGATCCATATTTCT+GGG	-	chr5.1:20947883-20947902	MS.gene036277:intron	30.0%
AGAAGGCTGAAAATAATGAA+GGG	-	chr5.1:20949500-20949519	MS.gene036277:CDS	30.0%
AGAGATTGTCGTAATGTATA+TGG	-	chr5.1:20947819-20947838	MS.gene036277:intron	30.0%
AGATGTGATACCAAGTATAA+TGG	-	chr5.1:20948432-20948451	MS.gene036277:intron	30.0%
ATAGTTAGAATATGGGGATT+TGG	-	chr5.1:20948877-20948896	MS.gene036277:intron	30.0%
ATAGTTATTAGGGGTAGTTT+AGG	+	chr5.1:20947635-20947654	None:intergenic	30.0%
ATCATTTAGGAAAAACTCGA+TGG	-	chr5.1:20946864-20946883	MS.gene036277:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	20946072	20949542	20946072	ID=MS.gene036277
chr5.1	mRNA	20946072	20949542	20946072	ID=MS.gene036277.t1;Parent=MS.gene036277
chr5.1	exon	20949192	20949542	20949192	ID=MS.gene036277.t1.exon1;Parent=MS.gene036277.t1
chr5.1	CDS	20949192	20949542	20949192	ID=cds.MS.gene036277.t1;Parent=MS.gene036277.t1
chr5.1	exon	20948918	20949102	20948918	ID=MS.gene036277.t1.exon2;Parent=MS.gene036277.t1
chr5.1	CDS	20948918	20949102	20948918	ID=cds.MS.gene036277.t1;Parent=MS.gene036277.t1
chr5.1	exon	20948551	20948780	20948551	ID=MS.gene036277.t1.exon3;Parent=MS.gene036277.t1
chr5.1	CDS	20948551	20948780	20948551	ID=cds.MS.gene036277.t1;Parent=MS.gene036277.t1
chr5.1	exon	20948299	20948440	20948299	ID=MS.gene036277.t1.exon4;Parent=MS.gene036277.t1
chr5.1	CDS	20948299	20948440	20948299	ID=cds.MS.gene036277.t1;Parent=MS.gene036277.t1
chr5.1	exon	20946072	20946133	20946072	ID=MS.gene036277.t1.exon5;Parent=MS.gene036277.t1
chr5.1	CDS	20946072	20946133	20946072	ID=cds.MS.gene036277.t1;Parent=MS.gene036277.t1

Gene Sequence

>MS.gene036277

ATGTCTCCGACGGCGGTGGCTACGGAGGAGGAAGGGCGGCGCAAACTGTCGTCGACGATGGAACGGAATTACTTGGGATTGTCTGATTGTTCATCGGTGGATAGTTGTGATTCAACTCTCCCAAGTTTGTGTGATGAGAAAAAAGTGAACTTGAATTTGAAGGCTACTGAGTTGAGGTTGGGTCTTCCCGGATCTCAATCACCCGAGAGGGAAATGGATTCGGATTTTTACGTAACAAAGCTTGATGAGAAACCATTGTTCCCTTTACTTCCTGCAAAAGATGGTTTGCAGAAGAATGTGGTTTCGGGGAATAAGAGAGGATTTGCTGATACCGTTGATGGGTTTTCGCAGGTGATTAGTTTGATGAAGTTGGTTTATGCGGTGAGAAGTGCATGTTTTGTTTTAGTGGTTTAATGGTGTTTACAATATTTGTTATGCAGGGTAAGTTTAATGCTAATTCAGGGATTAACGTGATGCTATCGCCTAGACCTGGTGGAGCTCAGGCGAGCACAGTGAAAGAAATGCCGAGTAAGGTTTTGCAGGAAAGGCCTTGTGCAGCTAGAGGAACTACTGGTCATAATCAGGCAGGTGCTGCTTCTATCGCTGGTTGTGCACCGGCATCTAAGTAAGTAAAATCTATCAACTCAACTACTCAAGGATCTTAATTTACACAAGACTAGTAGCTGAACAAACATGTATGATTTGGTTTTTTGTGTTTCTAGATATCCTAATCCTGATATGGTAAATTTTCATATGTGACAGGGCACAAGTTGTTGGTTGGCCTCCTATAAGATCATTTAGGAAAAACTCGATGGCCAATGCTTCCAAGAACAACAATGATGAAGTGGATGGAAAACCAGGTCCCACTGCACTCTTTGTGAAGGTCAGCATGGATGGTGCTCCCTATCTAAGGAAGGTAGATCTAAGAACTTATACAACATATCAGCAACTATCTTCTGCCCTTGAGAAGATGTTCAGCTGTTTTACTCTAGGTGAGTTGTTATTTTAATTTATTTTAACAATAAAATTTGTTTTTGCTGATGATGAAGTTGGTTGTTGCTTTATTTTTGTTCTGAAATCTTTGGCGATTGTGTAATGGCAGGCCAGTGTGGTTCCCATGGAGCTCCAGGAAAAGAAATGATGAGTGAGAGCAAGCTGAGGGACCTTTTGCATGGTTCAGAATATGTTCTCACTTATGAAGATAAAGATGGAGACTGGATGCTTGTAGGGGACGTACCATGGGAGTAAGATGTTCTTTAAATTCTGGTTTTTGCATCTTTGTTTTCTCGCTATATTTTTTCACGTAATAGTAGTCTCTATTTTGCTTCTGAGACAATGGAGTAAATGCTTTTGCAAATATATAGTCTAAAAAAGTAAGTAATCATAATGATCAGCAATAATTGATTGATTTTGTAATTAATAAGTAATTGTAAGTCATAATGATCATTTGAGAGTCCAGAATTAGACTTGTACTCCTTCCGTTTATAAATGAGTGTCGTTTAAGGTTTCCGCATACAAATTAAGAAATACAAAAAGGTTGTCAAAAGACATAACCCTTTTCCTAAACTACCCCTAATAACTATTGGTGTGTTTCCTACAAAAGTAGAATATATTACCTTCGAAGTAGTGTACACAACATTCATTTGAGGGCATAATTGGAAAAGTTTCCATTGAAAAGTGAACATACATTCATTTTGAAACAATTTTTTTTTCTCTAAAGAGACACTCATTTTGAAACGAAGGGATTAGAGATTGTCGTAATGTATATGGAGCGAGTCCTACTTGTATGTCCCCCTTGGAAGTCCCGAAAGACCAATTGATCCATATTTCTGGGTTGACTTATTTTCTTCTACTTTCTACTTAAATCAATTTGCAAATTGAGACTATATCTTATATACAAATATTGTTGGGTTGAGTCTCAGGTTTACTGGTTAATCCAATTTCCATGTATACTCATAGGCACTCACATTTGTGGTTTAGAAGATTAATTAATTGCTTGTGTCTGTTCTTTGAGTGCTAGGTCTAGTGATCAATCATATGTTTTTTCATGCCAAACAGCGGTGGTATGATATCACTTTTATTTTTCAAATACTACCTAACATTCAAGGGTTCTTGTTTTATTAGTGATTCCTGAAATTTGCTCAACATGGGTAAGCACTAATATTACTCAATTTGATTCAAAATATTATGATAAAAGATCATTCGTCAAATTAAGAGATGGATATAGTGAATCGAGAAAGTTAATACTGCAAATAATTGAACTTGTAATTTTTAATCGAAAATTAGGGGGCTCATGTCATGGCTACCGAGAACAACTAATGGGCGCATAACATATTACAGATGCGAATTATAATTTTGACAGATGTGATACCAAGTATAATGGATATTGCATGCAATTGCTCATCTAAGTAACTTGCCACTTGCTTGAAAAAATATCAAGAAAATTTTCCCTTTTTAGAAAGCAAAGTTATTTTGAAATCACAGTTCAGCATGCACTATCCTTAGGTTTCTTCCTGTCTTTTCAATCCTGCTACAGAATTTTCTGGCTAATTCAATATATTTAAAAGTGTGAATCAATTCAATGTATTGAAGTGTATTTTGATTTTTGAATGTTGTTATTTTTGTCTTTTTTGCTGCATAATGTTGCTGGGATGTACTAAACTAATGCATTTGAGTTGGGACAAAAATGGAATATTGTAGATAATTATCTGCTTCATAACACATTGGCTATATGGCATTGGCAAATTAGTATGAATGGGGTGCGGGGAATTCAGTAGGGGTACTTGATGAAAATGTGAGGAGGGAATAGTTAGAATATGGGGATTTGGCATGGAGGGTGCATGTGCATGCCCCTCTAAGAAACTTCAGCTCATTTTTATTAAAAATAAAAATAAAAAATTCTTGTCCTTTGTTTCTCCCATCTTAAATGGATTTACAGTGTTTTTGTGTTCTGTAATGTGTCTATCTATATTTTTTTTGAAGAAATTCAGGTTCTAATCGAAGTGTCGTGTATGCTATGACTTGTTCATTAGTATCCAGACTGAAGAATTTGATCATGACCAGGATTGTGTTGCTGAATATGATTATTAATAATGCTAGCAACACACTTTTCAACACACTCTTTGATTAAAATTCGCATGCGGCCAACCAAAATAATATGGGACCCACGTAATATGGTGGGACAAGTGTGAATTTTACCCAATCAAAGAGAGTATGTTGAAAAGTGTGAGTGTGTTGCTAGCATAGTAGCACTCCTCTAGTATTATAGTTCTGTAATTTACGGGCTTGTCTATTTAGGCATTAAGTGTTGATGCAAATTCTAGTATCTTAAAACCAAGAAAATGGACTGAATGAATGACATAAGGTTTGCATCCCACTCACTAAATTGCAATTCCTTATGCATGTGCAGGATGTTCATTGATACTTGCAGAAGGCTGAAAATAATGAAGGGTTCTGACGCCATTGGGTTAG

Protein sequence

>MS.gene036277.t1

MSPTAVATEEEGRRKLSSTMERNYLGLSDCSSVDSCDSTLPSLCDEKKVNLNLKATELRLGLPGSQSPEREMDSDFYVTKLDEKPLFPLLPAKDGLQKNVVSGNKRGFADTVDGFSQGKFNANSGINVMLSPRPGGAQASTVKEMPSKVLQERPCAARGTTGHNQAGAASIAGCAPASKAQVVGWPPIRSFRKNSMANASKNNNDEVDGKPGPTALFVKVSMDGAPYLRKVDLRTYTTYQQLSSALEKMFSCFTLGQCGSHGAPGKEMMSESKLRDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFIDTCRRLKIMKGSDAIGL