Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015847.t1 | XP_003625515.1 | 99.3 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 6.50E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015847.t1 | O82201 | 90.8 | 141 | 13 | 0 | 1 | 141 | 1 | 141 | 2.8e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015847.t1 | G7L0L8 | 99.3 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.7e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75781 | MS.gene015847 | PPI |
| MS.gene021005 | MS.gene015847 | PPI |
| MS.gene017498 | MS.gene015847 | PPI |
| MS.gene64216 | MS.gene015847 | PPI |
| MS.gene64013 | MS.gene015847 | PPI |
| MS.gene27073 | MS.gene015847 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015847.t1 | MTR_7g100010 | 99.301 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.64e-104 | 293 |
| MS.gene015847.t1 | MTR_8g102180 | 99.301 | 143 | 1 | 0 | 1 | 143 | 1 | 143 | 4.64e-104 | 293 |
| MS.gene015847.t1 | MTR_5g098940 | 42.029 | 138 | 80 | 0 | 3 | 140 | 2 | 139 | 9.50e-39 | 129 |
| MS.gene015847.t1 | MTR_1g025190 | 47.482 | 139 | 73 | 0 | 3 | 141 | 2 | 140 | 1.17e-38 | 128 |
| MS.gene015847.t1 | MTR_5g087820 | 41.791 | 134 | 78 | 0 | 3 | 136 | 26 | 159 | 8.53e-35 | 119 |
| MS.gene015847.t1 | MTR_8g077600 | 37.162 | 148 | 80 | 3 | 3 | 141 | 2 | 145 | 1.15e-30 | 108 |
| MS.gene015847.t1 | MTR_8g077600 | 35.484 | 93 | 47 | 3 | 3 | 86 | 2 | 90 | 1.40e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015847.t1 | AT2G19790 | 90.141 | 142 | 14 | 0 | 1 | 142 | 1 | 142 | 2.48e-96 | 274 |
| MS.gene015847.t1 | AT1G47830 | 48.201 | 139 | 72 | 0 | 3 | 141 | 2 | 140 | 2.48e-39 | 129 |
| MS.gene015847.t1 | AT2G17380 | 41.727 | 139 | 81 | 0 | 3 | 141 | 2 | 140 | 1.62e-38 | 128 |
| MS.gene015847.t1 | AT4G35410 | 41.727 | 139 | 81 | 0 | 3 | 141 | 2 | 140 | 3.23e-37 | 125 |
| MS.gene015847.t1 | AT3G50860 | 39.583 | 144 | 82 | 1 | 3 | 141 | 2 | 145 | 5.28e-35 | 119 |
| MS.gene015847.t1 | AT3G50860 | 39.583 | 144 | 82 | 1 | 3 | 141 | 2 | 145 | 5.57e-35 | 119 |
| MS.gene015847.t1 | AT4G35410 | 45.361 | 97 | 53 | 0 | 3 | 99 | 2 | 98 | 2.05e-25 | 93.2 |
Find 21 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAATGAGCTTGCTATTT+TGG | 0.125124 | 4.3:-3280488 | MS.gene015847:intron |
| GAAACCATGGATCGACATTT+TGG | 0.210891 | 4.3:-3280445 | MS.gene015847:CDS |
| GGGGATCCGATTCGTGTTAA+TGG | 0.262612 | 4.3:-3281920 | MS.gene015847:CDS |
| GAAGAACGACGAACACTTGA+AGG | 0.412781 | 4.3:-3281844 | MS.gene015847:CDS |
| GCAACTACAAAATTATATAT+AGG | 0.415613 | 4.3:-3280878 | MS.gene015847:CDS |
| TGTTCACCATTAACACGAAT+CGG | 0.437301 | 4.3:+3281914 | None:intergenic |
| TATCATGTTTCATTTAGAAA+AGG | 0.473226 | 4.3:-3280224 | MS.gene015847:CDS |
| TTCATAATACTGAGCAAGAC+GGG | 0.525077 | 4.3:+3281879 | None:intergenic |
| TCTGACTCCTATTCAGCTGA+TGG | 0.529053 | 4.3:-3280137 | MS.gene015847:CDS |
| TTCCTGGTTGGAGTTGATGA+CGG | 0.536130 | 4.3:-3280837 | MS.gene015847:CDS |
| CGCCGTCATCAACTCCAACC+AGG | 0.570939 | 4.3:+3280835 | None:intergenic |
| CCATGGTTTCGACTAAGAGA+TGG | 0.572456 | 4.3:+3280458 | None:intergenic |
| GGTCATGAATGGTTGTATTG+TGG | 0.573456 | 4.3:-3280179 | MS.gene015847:CDS |
| CCTCGCCCGTAATGAAAACC+AGG | 0.578527 | 4.3:-3281803 | MS.gene015847:intron |
| GTTGCCAAAATGTCGATCCA+TGG | 0.581731 | 4.3:+3280441 | None:intergenic |
| GTGTTAATGGTGAACAAACA+AGG | 0.589164 | 4.3:-3281907 | MS.gene015847:CDS |
| TTGGAGGAAATGGTCATGAA+TGG | 0.600198 | 4.3:-3280190 | MS.gene015847:CDS |
| CCATCTCTTAGTCGAAACCA+TGG | 0.604583 | 4.3:-3280458 | MS.gene015847:CDS |
| ATTCATAATACTGAGCAAGA+CGG | 0.623435 | 4.3:+3281878 | None:intergenic |
| TTCGTCGTTCTTCAACAGTG+AGG | 0.697820 | 4.3:+3281854 | None:intergenic |
| GGTTGGAGTTGATGACGGCG+AGG | 0.701215 | 4.3:-3280832 | MS.gene015847:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAATCAATTTGATT+TGG | + | chr4.3:3280566-3280585 | None:intergenic | 10.0% |
| !! | ATGAAAATAAAAGAAAAGAA+TGG | + | chr4.3:3280275-3280294 | None:intergenic | 15.0% |
| !! | GAAATTGGAAAAAAAAAAAA+AGG | + | chr4.3:3280844-3280863 | None:intergenic | 15.0% |
| !! | ATATTTATCTACAATGAAAG+TGG | + | chr4.3:3281478-3281497 | None:intergenic | 20.0% |
| !! | ATTTAAACATGTGATGAAAT+TGG | + | chr4.3:3280859-3280878 | None:intergenic | 20.0% |
| !! | GCAACTACAAAATTATATAT+AGG | - | chr4.3:3281167-3281186 | MS.gene015847:intron | 20.0% |
| !! | TATCATGTTTCATTTAGAAA+AGG | - | chr4.3:3281821-3281840 | MS.gene015847:CDS | 20.0% |
| !!! | ACTGTGTTAATATTTTAGTT+GGG | - | chr4.3:3280816-3280835 | MS.gene015847:intron | 20.0% |
| !!! | ATTCTAGTTGATTTATACAA+AGG | - | chr4.3:3281260-3281279 | MS.gene015847:intron | 20.0% |
| !!! | TGAATATTTTTACTATAGAC+AGG | - | chr4.3:3280704-3280723 | MS.gene015847:intron | 20.0% |
| ! | AGAAACAAAAAATCAGTGTT+TGG | + | chr4.3:3281759-3281778 | None:intergenic | 25.0% |
| ! | ATTAGGTAAAATTGAAGGTA+AGG | + | chr4.3:3280313-3280332 | None:intergenic | 25.0% |
| ! | CAACTAAAATATTAACACAG+TGG | + | chr4.3:3280817-3280836 | None:intergenic | 25.0% |
| ! | GGAAGAAAACAATAGAATTA+TGG | + | chr4.3:3281711-3281730 | None:intergenic | 25.0% |
| ! | TAAATAAGACATAGATGTCT+TGG | - | chr4.3:3280668-3280687 | MS.gene015847:intron | 25.0% |
| ! | TAGATTATATAATCTGTGAG+TGG | - | chr4.3:3280904-3280923 | MS.gene015847:intron | 25.0% |
| ! | TGTGAATTAGGTAAAATTGA+AGG | + | chr4.3:3280318-3280337 | None:intergenic | 25.0% |
| ! | TTGAATAATAAAGAATGACG+AGG | + | chr4.3:3280947-3280966 | None:intergenic | 25.0% |
| !!! | AGAAAAGGTACATTTTATGT+TGG | - | chr4.3:3281836-3281855 | MS.gene015847:CDS | 25.0% |
| !!! | CACTGTGTTAATATTTTAGT+TGG | - | chr4.3:3280815-3280834 | MS.gene015847:intron | 25.0% |
| AAACGATGACAATGTGAATT+AGG | + | chr4.3:3280330-3280349 | None:intergenic | 30.0% | |
| AGAATTATGGAAGTAATGTG+AGG | + | chr4.3:3281698-3281717 | None:intergenic | 30.0% | |
| AGTAAGAGAAAGTCAAAAGA+TGG | + | chr4.3:3281022-3281041 | None:intergenic | 30.0% | |
| ATTCATAATACTGAGCAAGA+CGG | + | chr4.3:3280170-3280189 | None:intergenic | 30.0% | |
| ATTCTTTATTATTCAACCCG+AGG | - | chr4.3:3280951-3280970 | MS.gene015847:intron | 30.0% | |
| CGTTTATTATTATTCAACCC+TGG | - | chr4.3:3280345-3280364 | MS.gene015847:intron | 30.0% | |
| GCATATTGTGTTAAATAGTC+TGG | - | chr4.3:3281303-3281322 | MS.gene015847:intron | 30.0% | |
| GGTTTATACCAGAAACATAA+GGG | - | chr4.3:3281056-3281075 | MS.gene015847:intron | 30.0% | |
| TATAGACAGGAGATAATTGT+TGG | - | chr4.3:3280717-3280736 | MS.gene015847:intron | 30.0% | |
| TCTTACTCATATTGATGTGT+TGG | - | chr4.3:3281035-3281054 | MS.gene015847:intron | 30.0% | |
| TGAGTGGAATGAAATTGAAA+TGG | - | chr4.3:3280920-3280939 | MS.gene015847:intron | 30.0% | |
| TGGTTTATACCAGAAACATA+AGG | - | chr4.3:3281055-3281074 | MS.gene015847:intron | 30.0% | |
| TTAACAAGACTAAGAACTTC+AGG | + | chr4.3:3281424-3281443 | None:intergenic | 30.0% | |
| TTATTATTATTCAACCCTGG+AGG | - | chr4.3:3280348-3280367 | MS.gene015847:intron | 30.0% | |
| TTTCATAATCTATCATGGAG+AGG | + | chr4.3:3280520-3280539 | None:intergenic | 30.0% | |
| ! | ACATTTTATGTTGGAGGAAA+TGG | - | chr4.3:3281845-3281864 | MS.gene015847:CDS | 30.0% |
| ! | CATAAACACTTCTGAGAATA+TGG | - | chr4.3:3280446-3280465 | MS.gene015847:CDS | 30.0% |
| ! | CTAGTTGATTTATACAAAGG+CGG | - | chr4.3:3281263-3281282 | MS.gene015847:intron | 30.0% |
| ! | GCTGATTTCATAATCTATCA+TGG | + | chr4.3:3280525-3280544 | None:intergenic | 30.0% |
| !! | AAAGGTACATTTTATGTTGG+AGG | - | chr4.3:3281839-3281858 | MS.gene015847:CDS | 30.0% |
| !! | AACATACCTGGTTTTCATTA+CGG | + | chr4.3:3280251-3280270 | None:intergenic | 30.0% |
| AAGTCAATCCCTTATGTTTC+TGG | + | chr4.3:3281067-3281086 | None:intergenic | 35.0% | |
| AATCTATCATGGAGAGGTTA+TGG | + | chr4.3:3280514-3280533 | None:intergenic | 35.0% | |
| AGCAAACTTGAATCCAAAGA+TGG | + | chr4.3:3280984-3281003 | None:intergenic | 35.0% | |
| AGGTAAAATTGAAGGTAAGG+AGG | + | chr4.3:3280310-3280329 | None:intergenic | 35.0% | |
| GAAAAGAATGGAAACATACC+TGG | + | chr4.3:3280263-3280282 | None:intergenic | 35.0% | |
| GTGTTAATGGTGAACAAACA+AGG | - | chr4.3:3280138-3280157 | MS.gene015847:CDS | 35.0% | |
| TCTACAATGAAAGTGGTACA+TGG | + | chr4.3:3281471-3281490 | None:intergenic | 35.0% | |
| TGTTCACCATTAACACGAAT+CGG | + | chr4.3:3280134-3280153 | None:intergenic | 35.0% | |
| TTCATAATACTGAGCAAGAC+GGG | + | chr4.3:3280169-3280188 | None:intergenic | 35.0% | |
| TTGAAGCTAAGTTCAGAAGA+TGG | + | chr4.3:3281736-3281755 | None:intergenic | 35.0% | |
| ! | ATTATTCAACCCGAGGTTTT+AGG | - | chr4.3:3280958-3280977 | MS.gene015847:intron | 35.0% |
| ! | GTTTTATCCATCAGCTGAAT+AGG | + | chr4.3:3281918-3281937 | None:intergenic | 35.0% |
| ! | TGGCTGATACATTTTTGTCA+TGG | - | chr4.3:3281121-3281140 | MS.gene015847:intron | 35.0% |
| !! | ACATACCTGGTTTTCATTAC+GGG | + | chr4.3:3280250-3280269 | None:intergenic | 35.0% |
| !!! | CTATGCATCTTTGTTTTTCC+TGG | - | chr4.3:3281192-3281211 | MS.gene015847:intron | 35.0% |
| AAAAGATGGCCTAAAACCTC+GGG | + | chr4.3:3281008-3281027 | None:intergenic | 40.0% | |
| ACTTTCTCTTCCCCAATTCT+GGG | + | chr4.3:3280755-3280774 | None:intergenic | 40.0% | |
| AGCTAAGTTCAGAAGATGGA+AGG | + | chr4.3:3281732-3281751 | None:intergenic | 40.0% | |
| CAAAAGATGGCCTAAAACCT+CGG | + | chr4.3:3281009-3281028 | None:intergenic | 40.0% | |
| CAATCTAATTGCTACCTCCA+GGG | + | chr4.3:3280365-3280384 | None:intergenic | 40.0% | |
| CCAGTTTCTTTAGACAAGCA+AGG | + | chr4.3:3281374-3281393 | None:intergenic | 40.0% | |
| CCTTGCTTGTCTAAAGAAAC+TGG | - | chr4.3:3281371-3281390 | MS.gene015847:intron | 40.0% | |
| GGTCATGAATGGTTGTATTG+TGG | - | chr4.3:3281866-3281885 | MS.gene015847:CDS | 40.0% | |
| TACTTTCTCTTCCCCAATTC+TGG | + | chr4.3:3280756-3280775 | None:intergenic | 40.0% | |
| TCAATCTAATTGCTACCTCC+AGG | + | chr4.3:3280366-3280385 | None:intergenic | 40.0% | |
| TCTGCAAAATTGTCGACAGA+AGG | + | chr4.3:3281542-3281561 | None:intergenic | 40.0% | |
| TGTATCAGCCAGATGAATCA+TGG | + | chr4.3:3281112-3281131 | None:intergenic | 40.0% | |
| TTCCTTTCACACCCAGAATT+GGG | - | chr4.3:3280741-3280760 | MS.gene015847:intron | 40.0% | |
| TTGGAGGAAATGGTCATGAA+TGG | - | chr4.3:3281855-3281874 | MS.gene015847:CDS | 40.0% | |
| TTTCCTTTCACACCCAGAAT+TGG | - | chr4.3:3280740-3280759 | MS.gene015847:intron | 40.0% | |
| ! | GAAACCATGGATCGACATTT+TGG | - | chr4.3:3281600-3281619 | MS.gene015847:intron | 40.0% |
| !!! | GCAGAATGAGCTTGCTATTT+TGG | - | chr4.3:3281557-3281576 | MS.gene015847:intron | 40.0% |
| !!! | GCATCTTTGTTTTTCCTGGT+TGG | - | chr4.3:3281196-3281215 | MS.gene015847:intron | 40.0% |
| AGTGGTCATCACTGTGTGAT+CGG | - | chr4.3:3281343-3281362 | MS.gene015847:intron | 45.0% | |
| CAAAGATGGCCTAAAACCTC+GGG | + | chr4.3:3280970-3280989 | None:intergenic | 45.0% | |
| CCAAAGATGGCCTAAAACCT+CGG | + | chr4.3:3280971-3280990 | None:intergenic | 45.0% | |
| CCATCTCTTAGTCGAAACCA+TGG | - | chr4.3:3281587-3281606 | MS.gene015847:intron | 45.0% | |
| CCATGGTTTCGACTAAGAGA+TGG | + | chr4.3:3281590-3281609 | None:intergenic | 45.0% | |
| GAAGAACGACGAACACTTGA+AGG | - | chr4.3:3280201-3280220 | MS.gene015847:CDS | 45.0% | |
| GCTTTGATCTTACTGACCAG+TGG | - | chr4.3:3281325-3281344 | MS.gene015847:intron | 45.0% | |
| GTTGCCAAAATGTCGATCCA+TGG | + | chr4.3:3281607-3281626 | None:intergenic | 45.0% | |
| TCCTTTCACACCCAGAATTG+GGG | - | chr4.3:3280742-3280761 | MS.gene015847:intron | 45.0% | |
| TCTGACTCCTATTCAGCTGA+TGG | - | chr4.3:3281908-3281927 | MS.gene015847:CDS | 45.0% | |
| TCTGCTCACCATGATTCATC+TGG | - | chr4.3:3281101-3281120 | MS.gene015847:intron | 45.0% | |
| TTCGTCGTTCTTCAACAGTG+AGG | + | chr4.3:3280194-3280213 | None:intergenic | 45.0% | |
| !! | TTCCTGGTTGGAGTTGATGA+CGG | - | chr4.3:3281208-3281227 | MS.gene015847:intron | 45.0% |
| !!! | AGTTTGCTACCCGAGGTTTT+AGG | - | chr4.3:3280996-3281015 | MS.gene015847:intron | 45.0% |
| ACAGAGACTCTAGTCACAGC+AGG | + | chr4.3:3281639-3281658 | None:intergenic | 50.0% | |
| GATCACACAGTGATGACCAC+TGG | + | chr4.3:3281344-3281363 | None:intergenic | 50.0% | |
| TCCCCAATTCTGGGTGTGAA+AGG | + | chr4.3:3280746-3280765 | None:intergenic | 50.0% | |
| ! | GGGGATCCGATTCGTGTTAA+TGG | - | chr4.3:3280125-3280144 | MS.gene015847:CDS | 50.0% |
| !! | GGATCGACATTTTGGCAACG+TGG | - | chr4.3:3281608-3281627 | MS.gene015847:intron | 50.0% |
| !! | GGATTCAAGTTTGCTACCCG+AGG | - | chr4.3:3280989-3281008 | MS.gene015847:intron | 50.0% |
| !!! | CCGAGGTTTTAGGCCATCTT+TGG | - | chr4.3:3280968-3280987 | MS.gene015847:intron | 50.0% |
| CCTCGCCCGTAATGAAAACC+AGG | - | chr4.3:3280242-3280261 | MS.gene015847:intron | 55.0% | |
| !! | CCTGGTTTTCATTACGGGCG+AGG | + | chr4.3:3280245-3280264 | None:intergenic | 55.0% |
| CGCCGTCATCAACTCCAACC+AGG | + | chr4.3:3281213-3281232 | None:intergenic | 60.0% | |
| !! | GGTTGGAGTTGATGACGGCG+AGG | - | chr4.3:3281213-3281232 | MS.gene015847:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 3280123 | 3281944 | 3280123 | ID=MS.gene015847 |
| chr4.3 | mRNA | 3280123 | 3281944 | 3280123 | ID=MS.gene015847.t1;Parent=MS.gene015847 |
| chr4.3 | exon | 3281804 | 3281944 | 3281804 | ID=MS.gene015847.t1.exon1;Parent=MS.gene015847.t1 |
| chr4.3 | CDS | 3281804 | 3281944 | 3281804 | ID=cds.MS.gene015847.t1;Parent=MS.gene015847.t1 |
| chr4.3 | exon | 3280833 | 3280919 | 3280833 | ID=MS.gene015847.t1.exon2;Parent=MS.gene015847.t1 |
| chr4.3 | CDS | 3280833 | 3280919 | 3280833 | ID=cds.MS.gene015847.t1;Parent=MS.gene015847.t1 |
| chr4.3 | exon | 3280438 | 3280506 | 3280438 | ID=MS.gene015847.t1.exon3;Parent=MS.gene015847.t1 |
| chr4.3 | CDS | 3280438 | 3280506 | 3280438 | ID=cds.MS.gene015847.t1;Parent=MS.gene015847.t1 |
| chr4.3 | exon | 3280123 | 3280257 | 3280123 | ID=MS.gene015847.t1.exon4;Parent=MS.gene015847.t1 |
| chr4.3 | CDS | 3280123 | 3280257 | 3280123 | ID=cds.MS.gene015847.t1;Parent=MS.gene015847.t1 |
| Gene Sequence |
| Protein sequence |
