AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75781


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75781.t1 MTR_5g098940 99.375 160 1 0 33 192 2 161 3.17e-116 327
MS.gene75781.t1 MTR_5g087820 82.500 160 28 0 33 192 26 185 4.00e-99 285
MS.gene75781.t1 MTR_1g025190 54.286 140 64 0 33 172 2 141 1.25e-51 162
MS.gene75781.t1 MTR_7g100010 43.478 138 78 0 33 170 3 140 3.40e-39 131
MS.gene75781.t1 MTR_8g102180 43.478 138 78 0 33 170 3 140 3.40e-39 131
MS.gene75781.t1 MTR_8g077600 36.810 163 93 2 33 190 2 159 1.43e-30 110
MS.gene75781.t1 MTR_8g077600 34.884 86 51 1 36 116 5 90 5.34e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75781.t1 AT2G17380 91.875 160 13 0 33 192 2 161 3.06e-110 312
MS.gene75781.t1 AT4G35410 90.625 160 15 0 33 192 2 161 7.07e-108 306
MS.gene75781.t1 AT4G35410 91.753 97 8 0 33 129 2 98 2.81e-64 194
MS.gene75781.t1 AT1G47830 52.857 140 66 0 33 172 2 141 1.30e-50 160
MS.gene75781.t1 AT2G19790 42.446 139 80 0 33 171 3 141 1.92e-38 129
MS.gene75781.t1 AT3G50860 36.943 157 88 2 33 184 2 152 2.08e-29 107
MS.gene75781.t1 AT3G50860 37.410 139 82 1 33 166 2 140 2.74e-29 106

Find 30 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATAAAGTTGTTTATAAA+AGG 0.214674 5.3:-79777147 MS.gene75781:intron
GAGATTCTTGATCGGTATTT+TGG 0.252220 5.3:-79776943 MS.gene75781:CDS
AATTTCATCAAGTATATAAT+AGG 0.297509 5.3:+79776385 None:intergenic
TCTAATTGCTGGCGAGCTTC+AGG 0.306112 5.3:-79776363 MS.gene75781:CDS
CATTGTCTTTCAGGATTCAT+TGG 0.310542 5.3:-79776228 MS.gene75781:intron
ATCGCACAAGCTACTAAATA+AGG 0.320939 5.3:-79776164 None:intergenic
CTTCTTTCCTTCTGAGTGTA+AGG 0.334840 5.3:+79777405 None:intergenic
CTTGATGAAATTCTAATTGC+TGG 0.336106 5.3:-79776374 MS.gene75781:CDS
CAATTTGCAGGTCTGTGAAT+TGG 0.375542 5.3:-79776813 MS.gene75781:intron
CTCTACAAAATTACAGAGCT+TGG 0.403422 5.3:+79777177 None:intergenic
GGAAGGTTAGGCTCACAAAA+TGG 0.441892 5.3:-79777434 MS.gene75781:CDS
TGATGATGCTGACAACGAAT+TGG 0.451575 5.3:-79776995 MS.gene75781:CDS
CTTGATATTCAACTTCCATA+AGG 0.468058 5.3:-79776789 MS.gene75781:intron
GATAATATTACTCAAGGAAC+TGG 0.478446 5.3:+79776184 None:intergenic
TTAGTCGTCAAGGGAAGGTT+AGG 0.481414 5.3:-79777446 MS.gene75781:CDS
CACTCAGAAGGAAAGAAGTA+AGG 0.487202 5.3:-79777400 MS.gene75781:intron
AAAAGAATGCTGTTTCATCA+TGG 0.502606 5.3:+79777709 None:intergenic
TCTACAAAATTACAGAGCTT+GGG 0.509679 5.3:+79777178 None:intergenic
GTGCTTCTCATTAGTCGTCA+AGG 0.528040 5.3:-79777456 MS.gene75781:CDS
TGCTCGGTTGATAGCTGCAC+AGG 0.540072 5.3:-79776321 MS.gene75781:intron
TGTCTTTCAGGATTCATTGG+TGG 0.541167 5.3:-79776225 MS.gene75781:intron
TGCTTCTCATTAGTCGTCAA+GGG 0.558103 5.3:-79777455 MS.gene75781:CDS
AATTACAGAGCTTGGGAGCA+CGG 0.567062 5.3:+79777185 None:intergenic
GAATCACTCCACTGAGCTCA+CGG 0.569834 5.3:+79777212 None:intergenic
TCTCATTAGTCGTCAAGGGA+AGG 0.585870 5.3:-79777451 MS.gene75781:CDS
TTGTGCGATAATATTACTCA+AGG 0.602166 5.3:+79776178 None:intergenic
ATTGGTGGAGACTGCTAAGG+AGG 0.602252 5.3:-79776210 MS.gene75781:CDS
GTATTCACCTTACACTCAGA+AGG 0.607833 5.3:-79777412 MS.gene75781:CDS
TTCATTGGTGGAGACTGCTA+AGG 0.613718 5.3:-79776213 MS.gene75781:CDS
AAGGTAATCCGTGAGCTCAG+TGG 0.653418 5.3:-79777220 MS.gene75781:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCATCAAGTATATAAT+AGG + chr5.3:79777491-79777510 None:intergenic 15.0%
!! ATATTATATCCTTAAGAAAA+GGG + chr5.3:79776995-79777014 None:intergenic 15.0%
!! GAAATATATTACATTAAACT+TGG + chr5.3:79777303-79777322 None:intergenic 15.0%
!! GATATAAAGTTGTTTATAAA+AGG - chr5.3:79776726-79776745 MS.gene75781:intron 15.0%
!! TATATCCATAATTCAAAATA+AGG - chr5.3:79777129-79777148 MS.gene75781:intron 15.0%
!! TATATTATATCCTTAAGAAA+AGG + chr5.3:79776996-79777015 None:intergenic 15.0%
!! ATTATCTGTATGTATTAACA+TGG - chr5.3:79776541-79776560 MS.gene75781:intron 20.0%
!! TATTTCCATAATTCAAAACA+AGG - chr5.3:79777317-79777336 MS.gene75781:intron 20.0%
!!! ATCTCATATTTTTATTGACA+AGG - chr5.3:79776634-79776653 MS.gene75781:intron 20.0%
!!! GAACTTTTAAATGAAATGAA+TGG - chr5.3:79776306-79776325 MS.gene75781:intron 20.0%
!!! TTTTAAATGAAATGAATGGT+AGG - chr5.3:79776310-79776329 MS.gene75781:intron 20.0%
! AAGACAATGTCATACAAATT+TGG + chr5.3:79777634-79777653 None:intergenic 25.0%
! ACCATGAAAGGATTATTAAT+AGG + chr5.3:79777176-79777195 None:intergenic 25.0%
! AGATGTAAATACACATATCA+AGG + chr5.3:79776618-79776637 None:intergenic 25.0%
! ATCGCTTATTCTCTTTATTA+TGG - chr5.3:79776509-79776528 MS.gene75781:intron 25.0%
! ATTCCAAGTACAAAAATACA+AGG + chr5.3:79776234-79776253 None:intergenic 25.0%
! CATGATTAAAAACAAAGAAC+AGG + chr5.3:79777463-79777482 None:intergenic 25.0%
! GATATATATCGCATTAAACT+TGG + chr5.3:79777115-79777134 None:intergenic 25.0%
! GATTATTAATAGGAGTTACA+GGG + chr5.3:79777166-79777185 None:intergenic 25.0%
! TAATAAAGAGAATAAGCGAT+AGG + chr5.3:79776509-79776528 None:intergenic 25.0%
! TAGAAATCCTATTAACAACT+GGG + chr5.3:79776349-79776368 None:intergenic 25.0%
! TATTAATAATCCTTTCATGG+TGG - chr5.3:79777175-79777194 MS.gene75781:CDS 25.0%
! TCCTATTAATAATCCTTTCA+TGG - chr5.3:79777172-79777191 MS.gene75781:CDS 25.0%
! TTAGAAATCCTATTAACAAC+TGG + chr5.3:79776350-79776369 None:intergenic 25.0%
!! TGAAATGATTCATCATTTTG+TGG - chr5.3:79776908-79776927 MS.gene75781:intron 25.0%
!!! CTGTTCTTTGTTTTTAATCA+TGG - chr5.3:79777461-79777480 MS.gene75781:CDS 25.0%
!!! TCTTTGTTTTTAATCATGGA+AGG - chr5.3:79777465-79777484 MS.gene75781:CDS 25.0%
ACTAGACCAATAATAATGAC+AGG + chr5.3:79777426-79777445 None:intergenic 30.0%
ACTAGACTGAATATAAAGAC+AGG + chr5.3:79777238-79777257 None:intergenic 30.0%
AGCAGATATTCATTATACCA+TGG + chr5.3:79776824-79776843 None:intergenic 30.0%
CACAAAGTGTATCTACATTT+AGG + chr5.3:79776400-79776419 None:intergenic 30.0%
CTTGATATTCAACTTCCATA+AGG - chr5.3:79777084-79777103 MS.gene75781:intron 30.0%
CTTGATGAAATTCTAATTGC+TGG - chr5.3:79777499-79777518 MS.gene75781:intron 30.0%
GAATATAAAGACAGGTGATA+TGG + chr5.3:79777230-79777249 None:intergenic 30.0%
GATAATATTACTCAAGGAAC+TGG + chr5.3:79777692-79777711 None:intergenic 30.0%
GGATTATTAATAGGAGTTAC+AGG + chr5.3:79777167-79777186 None:intergenic 30.0%
TATTAACATGGTGATTGTGA+TGG - chr5.3:79776553-79776572 MS.gene75781:intron 30.0%
TCTACAAAATTACAGAGCTT+GGG + chr5.3:79776698-79776717 None:intergenic 30.0%
TTAAACTTGGACATACCTTA+TGG + chr5.3:79777102-79777121 None:intergenic 30.0%
TTGTGCGATAATATTACTCA+AGG + chr5.3:79777698-79777717 None:intergenic 30.0%
TTTGTATGACATTGTCTTTC+AGG - chr5.3:79777636-79777655 MS.gene75781:CDS 30.0%
! AACAGCATTCTTTTGTGTTT+TGG - chr5.3:79776173-79776192 MS.gene75781:CDS 30.0%
! ACAGCATTCTTTTGTGTTTT+GGG - chr5.3:79776174-79776193 MS.gene75781:CDS 30.0%
! ATCAATGGTCTTTCTCTTTT+GGG - chr5.3:79777576-79777595 MS.gene75781:intron 30.0%
! ATTTTGTGGAGATTCTTGAT+CGG - chr5.3:79776922-79776941 MS.gene75781:intron 30.0%
! TATCAATGGTCTTTCTCTTT+TGG - chr5.3:79777575-79777594 MS.gene75781:intron 30.0%
! TGATTGAATTCTGAAAGTCT+AGG - chr5.3:79776284-79776303 MS.gene75781:intron 30.0%
!! TCACCTTGTATTTTTGTACT+TGG - chr5.3:79776228-79776247 MS.gene75781:intron 30.0%
!!! GTAGGCCTTATTTTGAATTA+TGG + chr5.3:79777137-79777156 None:intergenic 30.0%
AAACAAAGAACAGGTCAGAA+GGG + chr5.3:79777266-79777285 None:intergenic 35.0%
AATAATAATGACAGGCGATG+TGG + chr5.3:79777418-79777437 None:intergenic 35.0%
ACATCGCCTGTCATTATTAT+TGG - chr5.3:79777417-79777436 MS.gene75781:CDS 35.0%
AGGATGTTTATCATGCAATG+TGG + chr5.3:79776598-79776617 None:intergenic 35.0%
CATGATCACAAACAAAGAAC+AGG + chr5.3:79777275-79777294 None:intergenic 35.0%
CATTGTCTTTCAGGATTCAT+TGG - chr5.3:79777645-79777664 MS.gene75781:CDS 35.0%
CTCTACAAAATTACAGAGCT+TGG + chr5.3:79776699-79776718 None:intergenic 35.0%
GCTTGATGTTTAATTGACCA+TGG - chr5.3:79776804-79776823 MS.gene75781:CDS 35.0%
GTGTATCTACATTTAGGAAG+AGG + chr5.3:79776394-79776413 None:intergenic 35.0%
GTGTCACACTAACTTGATAA+TGG - chr5.3:79777029-79777048 MS.gene75781:intron 35.0%
! AGCTCTGTAATTTTGTAGAG+TGG - chr5.3:79776699-79776718 MS.gene75781:intron 35.0%
! CTGTTCTTTGTTTGTGATCA+TGG - chr5.3:79777273-79777292 MS.gene75781:intron 35.0%
! GAGATTCTTGATCGGTATTT+TGG - chr5.3:79776930-79776949 MS.gene75781:intron 35.0%
! TCTTTGTTTGTGATCATGGA+AGG - chr5.3:79777277-79777296 MS.gene75781:intron 35.0%
! TGTAATTTTGTAGAGTGGAG+AGG - chr5.3:79776704-79776723 MS.gene75781:intron 35.0%
! TTGAAGAGTCCCTTTTCTTA+AGG - chr5.3:79776983-79777002 MS.gene75781:CDS 35.0%
!! CAGCTTTATAGTCTTTTGCA+AGG - chr5.3:79776767-79776786 MS.gene75781:intron 35.0%
!!! GTAGGCCTTGTTTTGAATTA+TGG + chr5.3:79777325-79777344 None:intergenic 35.0%
ATGGTCACTTGTCAATTTGC+AGG - chr5.3:79777048-79777067 MS.gene75781:intron 40.0%
CAAACAAAGAACAGGTCAGA+AGG + chr5.3:79777267-79777286 None:intergenic 40.0%
CAATTTGCAGGTCTGTGAAT+TGG - chr5.3:79777060-79777079 MS.gene75781:intron 40.0%
CACTCAGAAGGAAAGAAGTA+AGG - chr5.3:79776473-79776492 MS.gene75781:intron 40.0%
CGAGCAAAAAAACTGTTGCT+CGG - chr5.3:79777536-79777555 MS.gene75781:intron 40.0%
CTTCTTTCCTTCTGAGTGTA+AGG + chr5.3:79776471-79776490 None:intergenic 40.0%
GGAGTTACAGGGAAATAAGT+AGG + chr5.3:79777155-79777174 None:intergenic 40.0%
GGAGTTACAGGGAATTAAGT+AGG + chr5.3:79777343-79777362 None:intergenic 40.0%
GGTGATATGGTCAACAATCA+AGG + chr5.3:79777217-79777236 None:intergenic 40.0%
GTATTCACCTTACACTCAGA+AGG - chr5.3:79776461-79776480 MS.gene75781:intron 40.0%
TAATTGAGGCTCATTCAGTG+TGG + chr5.3:79777391-79777410 None:intergenic 40.0%
TGCTTCTCATTAGTCGTCAA+GGG - chr5.3:79776418-79776437 MS.gene75781:intron 40.0%
TGTCTTTCAGGATTCATTGG+TGG - chr5.3:79777648-79777667 MS.gene75781:CDS 40.0%
TTGCAAGGCAATCTTCTGTT+TGG - chr5.3:79776782-79776801 MS.gene75781:intron 40.0%
! TGATGATGCTGACAACGAAT+TGG - chr5.3:79776878-79776897 MS.gene75781:intron 40.0%
AATTACAGAGCTTGGGAGCA+CGG + chr5.3:79776691-79776710 None:intergenic 45.0%
CAATCAAGGCTCATTCAGTG+TGG + chr5.3:79777203-79777222 None:intergenic 45.0%
CCTATTAACAACTGGGAGAG+AGG + chr5.3:79776342-79776361 None:intergenic 45.0%
CCTCTCTCCCAGTTGTTAAT+AGG - chr5.3:79776339-79776358 MS.gene75781:CDS 45.0%
GATAGCTGCACAGGTATCAA+TGG - chr5.3:79777561-79777580 MS.gene75781:intron 45.0%
GGAAGGTTAGGCTCACAAAA+TGG - chr5.3:79776439-79776458 MS.gene75781:intron 45.0%
GGCGATGTGGTCAATAATTG+AGG + chr5.3:79777405-79777424 None:intergenic 45.0%
GTGCTTCTCATTAGTCGTCA+AGG - chr5.3:79776417-79776436 MS.gene75781:intron 45.0%
TCTCATTAGTCGTCAAGGGA+AGG - chr5.3:79776422-79776441 MS.gene75781:intron 45.0%
TTAGTCGTCAAGGGAAGGTT+AGG - chr5.3:79776427-79776446 MS.gene75781:intron 45.0%
TTCATTGGTGGAGACTGCTA+AGG - chr5.3:79777660-79777679 MS.gene75781:CDS 45.0%
AAGGTAATCCGTGAGCTCAG+TGG - chr5.3:79776653-79776672 MS.gene75781:intron 50.0%
ATTGGTGGAGACTGCTAAGG+AGG - chr5.3:79777663-79777682 MS.gene75781:CDS 50.0%
CAGTGTGGATCCACCATGAA+AGG + chr5.3:79777188-79777207 None:intergenic 50.0%
GAATCACTCCACTGAGCTCA+CGG + chr5.3:79776664-79776683 None:intergenic 50.0%
TCTAATTGCTGGCGAGCTTC+AGG - chr5.3:79777510-79777529 MS.gene75781:intron 50.0%
!! TGCTCGGTTGATAGCTGCAC+AGG - chr5.3:79777552-79777571 MS.gene75781:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 79776166 79777729 79776166 ID=MS.gene75781
chr5.3 mRNA 79776166 79777729 79776166 ID=MS.gene75781.t1;Parent=MS.gene75781
chr5.3 exon 79777634 79777729 79777634 ID=MS.gene75781.t1.exon1;Parent=MS.gene75781.t1
chr5.3 CDS 79777634 79777729 79777634 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79777401 79777487 79777401 ID=MS.gene75781.t1.exon2;Parent=MS.gene75781.t1
chr5.3 CDS 79777401 79777487 79777401 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79777148 79777239 79777148 ID=MS.gene75781.t1.exon3;Parent=MS.gene75781.t1
chr5.3 CDS 79777148 79777239 79777148 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79776939 79777047 79776939 ID=MS.gene75781.t1.exon4;Parent=MS.gene75781.t1
chr5.3 CDS 79776939 79777047 79776939 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79776790 79776825 79776790 ID=MS.gene75781.t1.exon5;Parent=MS.gene75781.t1
chr5.3 CDS 79776790 79776825 79776790 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79776322 79776408 79776322 ID=MS.gene75781.t1.exon6;Parent=MS.gene75781.t1
chr5.3 CDS 79776322 79776408 79776322 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
chr5.3 exon 79776166 79776237 79776166 ID=MS.gene75781.t1.exon7;Parent=MS.gene75781.t1
chr5.3 CDS 79776166 79776237 79776166 ID=cds.MS.gene75781.t1;Parent=MS.gene75781.t1
Gene Sequence

>MS.gene75781

ATGATGAAACAGCATTCTTTTGTGTTTTGGGTTTCTGTTACTATCAATCATGAAGTTGATTTTCACCTTGTATTTTTGTACTTGGAATTCATATATGTACGAATGCTTATTTATTATCTGATTGAATTCTGAAAGTCTAGGAACTTTTAAATGAAATGAATGGTAGGATTGTGCCTCTCTCCCAGTTGTTAATAGGATTTCTAACTTCAACTTGCATGCTTTATTCCTCTTCCTAAATGTAGATACACTTTGTGCTTCTCATTAGTCGTCAAGGGAAGGTTAGGCTCACAAAATGGTATTCACCTTACACTCAGAAGGAAAGAAGTAAGGTAACTAACAACCTATCGCTTATTCTCTTTATTATGGAAATATGTTATTATCTGTATGTATTAACATGGTGATTGTGATGGTCATTGCATGCTGAGCACACCACATTGCATGATAAACATCCTTGATATGTGTATTTACATCTCATATTTTTATTGACAAGGTAATCCGTGAGCTCAGTGGAGTGATTCTTAGCCGTGCTCCCAAGCTCTGTAATTTTGTAGAGTGGAGAGGATATAAAGTTGTTTATAAAAGGTATTTTGACTAGAGTAGACAGCTTTATAGTCTTTTGCAAGGCAATCTTCTGTTTGGCTTGATGTTTAATTGACCATGGTATAATGAATATCTGCTGCAGATATGCTAGTCTATATTTCTGTATGTGTATTGATGATGCTGACAACGAATTGGAAGTACTTGAAATGATTCATCATTTTGTGGAGATTCTTGATCGGTATTTTGGCAGTGTAAGTTCTATCAAAAGTTTTGACTTTTGAAGAGTCCCTTTTCTTAAGGATATAATATAAGAAGCATTTCTCGTGTCACACTAACTTGATAATGGTCACTTGTCAATTTGCAGGTCTGTGAATTGGACTTGATATTCAACTTCCATAAGGTATGTCCAAGTTTAATGCGATATATATCCATAATTCAAAATAAGGCCTACTTATTTCCCTGTAACTCCTATTAATAATCCTTTCATGGTGGATCCACACTGAATGAGCCTTGATTGTTGACCATATCACCTGTCTTTATATTCAGTCTAGTAATCTCCCTTCTGACCTGTTCTTTGTTTGTGATCATGGAAGGCCAAGTTTAATGTAATATATTTCCATAATTCAAAACAAGGCCTACTTAATTCCCTGTAACTCCTATTAATAATCCTTTCATGGTGGATCCACACTGAATGAGCCTCAATTATTGACCACATCGCCTGTCATTATTATTGGTCTAGTAATCTCGCTTCTGACCTGTTCTTTGTTTTTAATCATGGAAGGCCTATTATATACTTGATGAAATTCTAATTGCTGGCGAGCTTCAGGAGTCGAGCAAAAAAACTGTTGCTCGGTTGATAGCTGCACAGGTATCAATGGTCTTTCTCTTTTGGGTATGTTTTGATTCTTCAATCAGTTTTTCTAATCCAAATTTGTATGACATTGTCTTTCAGGATTCATTGGTGGAGACTGCTAAGGAGGAAGCCAGTTCCTTGAGTAATATTATCGCACAAGCTACTAAATAA

Protein sequence

>MS.gene75781.t1

MMKQHSFVFWVSVTINHEVDFHLVFLYLEFIYIHFVLLISRQGKVRLTKWYSPYTQKERSKVIRELSGVILSRAPKLCNFVEWRGYKVVYKRYASLYFCMCIDDADNELEVLEMIHHFVEILDRYFGSVCELDLIFNFHKAYYILDEILIAGELQESSKKTVARLIAAQDSLVETAKEEASSLSNIIAQATK