Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016334.t1 | XP_003628195.1 | 98.4 | 489 | 8 | 0 | 1 | 489 | 1 | 489 | 1.10E-277 | 965.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016334.t1 | Q5E915 | 70.1 | 485 | 140 | 3 | 1 | 485 | 1 | 480 | 6.9e-196 | 684.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016334.t1 | G7L7T0 | 98.4 | 489 | 8 | 0 | 1 | 489 | 1 | 489 | 7.9e-278 | 965.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60096 | MS.gene016334 | PPI |
| MS.gene016334 | MS.gene21793 | PPI |
| MS.gene016334 | MS.gene058069 | PPI |
| MS.gene016334 | MS.gene40494 | PPI |
| MS.gene016334 | MS.gene90863 | PPI |
| MS.gene016334 | MS.gene75146 | PPI |
| MS.gene024821 | MS.gene016334 | PPI |
| MS.gene016334 | MS.gene056221 | PPI |
| MS.gene016334 | MS.gene037409 | PPI |
| MS.gene016615 | MS.gene016334 | PPI |
| MS.gene016334 | MS.gene88509 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016334.t1 | MTR_8g045440 | 98.364 | 489 | 8 | 0 | 1 | 489 | 1 | 489 | 0.0 | 984 |
| MS.gene016334.t1 | MTR_8g041740 | 93.252 | 489 | 33 | 0 | 1 | 489 | 1 | 489 | 0.0 | 910 |
| MS.gene016334.t1 | MTR_8g045463 | 92.292 | 493 | 36 | 1 | 7 | 497 | 7 | 499 | 0.0 | 899 |
| MS.gene016334.t1 | MTR_8g045470 | 92.292 | 493 | 36 | 1 | 7 | 497 | 7 | 499 | 0.0 | 899 |
| MS.gene016334.t1 | MTR_8g045440 | 98.015 | 403 | 8 | 0 | 1 | 403 | 1 | 403 | 0.0 | 809 |
| MS.gene016334.t1 | MTR_5g069620 | 71.429 | 84 | 24 | 0 | 38 | 121 | 8 | 91 | 5.77e-35 | 126 |
| MS.gene016334.t1 | MTR_7g086360 | 25.000 | 340 | 191 | 12 | 29 | 362 | 61 | 342 | 1.25e-15 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016334.t1 | AT3G21060 | 70.226 | 487 | 140 | 3 | 1 | 487 | 1 | 482 | 0.0 | 676 |
| MS.gene016334.t1 | AT2G43770 | 23.824 | 340 | 195 | 13 | 29 | 362 | 59 | 340 | 1.50e-13 | 72.4 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGTGATTGAAGAATATT+TGG | 0.157133 | 8.2:-56562104 | MS.gene016334:CDS |
| ATGATGGAAGCTGTGTTATT+TGG | 0.213605 | 8.2:-56557615 | MS.gene016334:CDS |
| TTCATATTCTTGCAGGTAAA+AGG | 0.213606 | 8.2:-56556468 | MS.gene016334:intron |
| GCTACCACTTCTTCCGAAAA+TGG | 0.216044 | 8.2:+56556255 | None:intergenic |
| TGTGTGGCATAGTTTATATC+TGG | 0.225456 | 8.2:-56556676 | MS.gene016334:CDS |
| CTCTCCATCACCACTGAAAC+AGG | 0.282312 | 8.2:+56556850 | None:intergenic |
| TCCTCAATCTCTTTGAAATC+AGG | 0.297441 | 8.2:+56556617 | None:intergenic |
| AATGCTCTAACTTCATCTTT+TGG | 0.301792 | 8.2:+56556998 | None:intergenic |
| AAATATTCTTCAATCACTTC+TGG | 0.306044 | 8.2:+56562106 | None:intergenic |
| CCAGTTCTCTATGTACTCTT+TGG | 0.307118 | 8.2:+56556652 | None:intergenic |
| ATATCAGAGTCGCTGAAATT+AGG | 0.326868 | 8.2:+56556393 | None:intergenic |
| TGCCAAGCAGAGAGATGATT+TGG | 0.342619 | 8.2:+56557381 | None:intergenic |
| ACCGATAATGGAGTTGTTCA+TGG | 0.343174 | 8.2:+56562401 | None:intergenic |
| TAGATAGTTCTTCGAAGATT+GGG | 0.351030 | 8.2:-56556299 | MS.gene016334:CDS |
| GATGCCACGCTAAATCAATA+AGG | 0.351990 | 8.2:+56556729 | None:intergenic |
| AAAAGCCTATCTTCCTGTTC+AGG | 0.357336 | 8.2:+56556321 | None:intergenic |
| GGTGATGGAGAGTGGGTTGT+TGG | 0.362512 | 8.2:-56556839 | MS.gene016334:CDS |
| CTCCCATCACCAGTATTTGT+TGG | 0.370774 | 8.2:-56557540 | MS.gene016334:CDS |
| CGTGGCATGGTTTCTATCTC+TGG | 0.372952 | 8.2:-56557121 | MS.gene016334:CDS |
| TTAGATAGTTCTTCGAAGAT+TGG | 0.374218 | 8.2:-56556300 | MS.gene016334:CDS |
| TGATGGAAGCTGTGTTATTT+GGG | 0.380302 | 8.2:-56557614 | MS.gene016334:CDS |
| GAGAACCTCGGTGATCTAAA+TGG | 0.383675 | 8.2:-56556962 | MS.gene016334:CDS |
| TTTGCTAGCTCGTCTTCATC+CGG | 0.385250 | 8.2:-56557410 | MS.gene016334:CDS |
| GGATGTTGAAGATCCGTTGC+AGG | 0.390495 | 8.2:-56562137 | MS.gene016334:intron |
| GAACCCTTGTTGCGTGAGAC+AGG | 0.398687 | 8.2:+56557262 | None:intergenic |
| ACCATGAACAACTCCATTAT+CGG | 0.399710 | 8.2:-56562402 | MS.gene016334:intron |
| CATTCCTGATGTTCCTGAAC+AGG | 0.399964 | 8.2:-56556334 | MS.gene016334:CDS |
| ACATTTGTTGCGCTTATTTG+AGG | 0.406787 | 8.2:-56555517 | None:intergenic |
| ACACTTTGACCAACAAATAC+TGG | 0.409133 | 8.2:+56557531 | None:intergenic |
| GGGCTTCCTTAGGCCCTTCA+AGG | 0.426153 | 8.2:+56556750 | None:intergenic |
| TTGCTAGCTCGTCTTCATCC+GGG | 0.427639 | 8.2:-56557409 | MS.gene016334:CDS |
| CAAAGAGATTGAGGAAAATG+AGG | 0.433376 | 8.2:-56556609 | MS.gene016334:CDS |
| CTTGTAATTGACAGTAAAGA+TGG | 0.434768 | 8.2:-56557151 | MS.gene016334:CDS |
| AAAGAAACAGACACAACAAT+TGG | 0.439804 | 8.2:+56556698 | None:intergenic |
| TTGTTGCGTGAGACAGGGTT+TGG | 0.440027 | 8.2:+56557268 | None:intergenic |
| TGAGGAAAATGAGGAGTATG+TGG | 0.443627 | 8.2:-56556600 | MS.gene016334:CDS |
| TCCTGATTTCAAAGAGATTG+AGG | 0.446776 | 8.2:-56556618 | MS.gene016334:CDS |
| GTGTGGCATAGTTTATATCT+GGG | 0.447441 | 8.2:-56556675 | MS.gene016334:CDS |
| GTGTTCAAGTCAACAATCAT+TGG | 0.449074 | 8.2:+56557340 | None:intergenic |
| GGGATGTCTTGAGTGGTAAA+AGG | 0.450323 | 8.2:-56557462 | MS.gene016334:CDS |
| CTCCAAATCATCTCTCTGCT+TGG | 0.452595 | 8.2:-56557383 | MS.gene016334:CDS |
| CATTTGTTGCGCTTATTTGA+GGG | 0.457692 | 8.2:-56555516 | None:intergenic |
| TCTTCCTGTTCAGGAACATC+AGG | 0.459403 | 8.2:+56556330 | None:intergenic |
| ATGTTCCTGAACAGGAAGAT+AGG | 0.459550 | 8.2:-56556326 | MS.gene016334:CDS |
| GATGGAGAGTGGGTTGTTGG+TGG | 0.468537 | 8.2:-56556836 | MS.gene016334:CDS |
| CATGCCTCAAGTCCACTTGA+AGG | 0.471872 | 8.2:-56556213 | MS.gene016334:intron |
| TCACATACATTATCAGTGTC+AGG | 0.473182 | 8.2:+56556551 | None:intergenic |
| GAGAGCACAAGATTTACATA+TGG | 0.478073 | 8.2:-56556799 | MS.gene016334:CDS |
| CGTCGCGGTACCCTTCTTGC+TGG | 0.478409 | 8.2:-56562049 | MS.gene016334:intron |
| TCGGAAGAAGTGGTAGCAAA+TGG | 0.478453 | 8.2:-56556249 | MS.gene016334:CDS |
| GAATCAGCTGGCTGCAATGA+TGG | 0.479058 | 8.2:-56557631 | MS.gene016334:intron |
| CATCTTGTGAAAATCCTTGA+AGG | 0.482006 | 8.2:-56556764 | MS.gene016334:CDS |
| CCAAAGAGTACATAGAGAAC+TGG | 0.483830 | 8.2:-56556652 | MS.gene016334:CDS |
| GTATGGAAACCTGGTTTATG+TGG | 0.490370 | 8.2:-56557194 | MS.gene016334:CDS |
| TTGAAGAATATTTGGAACAT+GGG | 0.501031 | 8.2:-56562096 | MS.gene016334:CDS |
| TGACCAACAAATACTGGTGA+TGG | 0.505055 | 8.2:+56557537 | None:intergenic |
| GACACAACAATTGGACGTAC+AGG | 0.508623 | 8.2:+56556707 | None:intergenic |
| TGAAGAACATTGTATTCAGC+AGG | 0.511366 | 8.2:-56557087 | MS.gene016334:CDS |
| ATGCCACGCTAAATCAATAA+GGG | 0.511908 | 8.2:+56556730 | None:intergenic |
| CTGTTCAGGAACATCAGGAA+TGG | 0.514821 | 8.2:+56556335 | None:intergenic |
| AACATTGTATTCAGCAGGAA+TGG | 0.517859 | 8.2:-56557082 | MS.gene016334:CDS |
| GAGACAGGGTTTGGTCCACT+TGG | 0.523067 | 8.2:+56557277 | None:intergenic |
| TCACGCAACAAGGGTTCTGA+TGG | 0.523362 | 8.2:-56557256 | MS.gene016334:CDS |
| AAATCAATAAGGGCTTCCTT+AGG | 0.523475 | 8.2:+56556740 | None:intergenic |
| TGTTCAGGAACATCAGGAAT+GGG | 0.524926 | 8.2:+56556336 | None:intergenic |
| GCACCCTGTTTCAGTGGTGA+TGG | 0.531362 | 8.2:-56556854 | MS.gene016334:CDS |
| GATGAAGACGAGCTAGCAAA+GGG | 0.534466 | 8.2:+56557413 | None:intergenic |
| GTTTCAGTGGTGATGGAGAG+TGG | 0.534496 | 8.2:-56556847 | MS.gene016334:CDS |
| ATTGAAGAATATTTGGAACA+TGG | 0.536169 | 8.2:-56562097 | MS.gene016334:CDS |
| GTTGTGTCTGTTTCTTTGTG+TGG | 0.539897 | 8.2:-56556692 | MS.gene016334:CDS |
| CAAGCAGAGAGATGATTTGG+AGG | 0.545050 | 8.2:+56557384 | None:intergenic |
| ATGCAATCACGAAAGTGCAC+TGG | 0.552939 | 8.2:-56556880 | MS.gene016334:CDS |
| AGAGCACAAGATTTACATAT+GGG | 0.555323 | 8.2:-56556798 | MS.gene016334:CDS |
| GAAAATCCTTGAAGGGCCTA+AGG | 0.556664 | 8.2:-56556756 | MS.gene016334:CDS |
| AACCCTTGTTGCGTGAGACA+GGG | 0.563354 | 8.2:+56557263 | None:intergenic |
| TGCTAGCTCGTCTTCATCCG+GGG | 0.567258 | 8.2:-56557408 | MS.gene016334:CDS |
| CTTCAAGTGGACTTGAGGCA+TGG | 0.571372 | 8.2:+56556214 | None:intergenic |
| AGATAGTTCTTCGAAGATTG+GGG | 0.573049 | 8.2:-56556298 | MS.gene016334:CDS |
| AAACCCTGTCTCACGCAACA+AGG | 0.574516 | 8.2:-56557266 | MS.gene016334:CDS |
| ATTGGGGACAGCAATAACTC+TGG | 0.576784 | 8.2:-56556282 | MS.gene016334:CDS |
| TTTCAGTGGTGATGGAGAGT+GGG | 0.579791 | 8.2:-56556846 | MS.gene016334:CDS |
| TGGAAGGCACCCTGTTTCAG+TGG | 0.579970 | 8.2:-56556860 | MS.gene016334:CDS |
| ATTTACATATGGGATAGAGC+TGG | 0.580981 | 8.2:-56556788 | MS.gene016334:CDS |
| ATTACCTTCAAGTGGACTTG+AGG | 0.584311 | 8.2:+56556209 | None:intergenic |
| TCGAGTTTCCCACATAAACC+AGG | 0.588345 | 8.2:+56557185 | None:intergenic |
| AAATGGTGTTGAGAAGTTGA+AGG | 0.591187 | 8.2:-56556945 | MS.gene016334:CDS |
| ATCTGATGTCAATGAAGATG+AGG | 0.592161 | 8.2:-56556445 | MS.gene016334:CDS |
| GTTGGTGGTTCTGCTAGCAA+AGG | 0.593351 | 8.2:-56556821 | MS.gene016334:CDS |
| GGCGTGACATTACCTTCAAG+TGG | 0.593844 | 8.2:+56556201 | None:intergenic |
| TTACTAAAACATGCAGCAGT+AGG | 0.595182 | 8.2:+56557217 | None:intergenic |
| GACCAACAAATACTGGTGAT+GGG | 0.601286 | 8.2:+56557538 | None:intergenic |
| TAGATAACGTAGCTCTTCCT+CGG | 0.601900 | 8.2:+56556368 | None:intergenic |
| GGATGAAGACGAGCTAGCAA+AGG | 0.602138 | 8.2:+56557412 | None:intergenic |
| ATCTTGTGAAAATCCTTGAA+GGG | 0.602213 | 8.2:-56556763 | MS.gene016334:CDS |
| GGCATGGTTTCTATCTCTGG+TGG | 0.603497 | 8.2:-56557118 | MS.gene016334:CDS |
| ACATGCAGCAGTAGGTGTGA+AGG | 0.605285 | 8.2:+56557225 | None:intergenic |
| AGATGGTGAGGTGCGTGGCA+TGG | 0.609126 | 8.2:-56557134 | MS.gene016334:CDS |
| TATGGAAACCTGGTTTATGT+GGG | 0.609917 | 8.2:-56557193 | MS.gene016334:CDS |
| TGACAAGTCGTTATCCCTGT+GGG | 0.614779 | 8.2:-56557482 | MS.gene016334:CDS |
| TACCACTCAAGACATCCCAC+AGG | 0.615382 | 8.2:+56557467 | None:intergenic |
| AACCCTGTCTCACGCAACAA+GGG | 0.615851 | 8.2:-56557265 | MS.gene016334:CDS |
| AAATAAGCGCAACAAATGTG+TGG | 0.618207 | 8.2:+56555521 | None:intergenic |
| GATGTTGAAGATCCGTTGCA+GGG | 0.618600 | 8.2:-56562136 | MS.gene016334:intron |
| AGAGATGATTTGGAGGACCC+CGG | 0.619630 | 8.2:+56557391 | None:intergenic |
| TGACATTGTGACAGTCGAGA+AGG | 0.620507 | 8.2:-56556418 | MS.gene016334:CDS |
| TCTGGAAAATCACCCTGCAA+CGG | 0.620523 | 8.2:+56562124 | None:intergenic |
| TTAATATACAGCAAGGCCAA+TGG | 0.622591 | 8.2:-56555554 | MS.gene016334:intron |
| ACATCAGGAATGGGAGACAC+TGG | 0.624457 | 8.2:+56556345 | None:intergenic |
| AGTATTTGTTGGTCAAAGTG+TGG | 0.624701 | 8.2:-56557529 | MS.gene016334:CDS |
| CTGACAAGTCGTTATCCCTG+TGG | 0.627369 | 8.2:-56557483 | MS.gene016334:CDS |
| GTTTATGTGGGAAACTCGAA+AGG | 0.631466 | 8.2:-56557181 | MS.gene016334:CDS |
| TGATTGCATCTTGAAACTCA+CGG | 0.633891 | 8.2:+56556894 | None:intergenic |
| ATCATTGGAGCACATGAGAG+AGG | 0.635797 | 8.2:+56557355 | None:intergenic |
| TCTCCATCACCACTGAAACA+GGG | 0.640101 | 8.2:+56556851 | None:intergenic |
| TCCCTGTGGGATGTCTTGAG+TGG | 0.645082 | 8.2:-56557469 | MS.gene016334:CDS |
| TCTGTCTCTGACAAACCAAG+TGG | 0.648181 | 8.2:-56557292 | MS.gene016334:CDS |
| AAGCCCTTATTGATTTAGCG+TGG | 0.655079 | 8.2:-56556733 | MS.gene016334:CDS |
| TGCAGCAGTAGGTGTGAAGG+AGG | 0.657772 | 8.2:+56557228 | None:intergenic |
| ACCACTCAAGACATCCCACA+GGG | 0.671936 | 8.2:+56557468 | None:intergenic |
| CAGCGACTCTGATATGTCCG+AGG | 0.676024 | 8.2:-56556385 | MS.gene016334:CDS |
| GCTGAATTGAACGAGAACCT+CGG | 0.676455 | 8.2:-56556974 | MS.gene016334:CDS |
| GTTGAGAAGTTGAAGGCTGT+AGG | 0.680893 | 8.2:-56556938 | MS.gene016334:CDS |
| AGTAAAGATGGTGAGGTGCG+TGG | 0.690847 | 8.2:-56557139 | MS.gene016334:CDS |
| AATCACGAAAGTGCACTGGA+AGG | 0.694138 | 8.2:-56556876 | MS.gene016334:CDS |
| TGTATTGCTTTCAATCGTCG+CGG | 0.697076 | 8.2:-56562064 | MS.gene016334:CDS |
| AATTGACAGTAAAGATGGTG+AGG | 0.704790 | 8.2:-56557146 | MS.gene016334:CDS |
| CAACACCATTTAGATCACCG+AGG | 0.738329 | 8.2:+56556957 | None:intergenic |
| ATGAAGACGAGCTAGCAAAG+GGG | 0.760208 | 8.2:+56557414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 56555519 | 56562421 | 56555519 | ID=MS.gene016334 |
| chr8.2 | mRNA | 56555519 | 56562421 | 56555519 | ID=MS.gene016334.t1;Parent=MS.gene016334 |
| chr8.2 | exon | 56562403 | 56562421 | 56562403 | ID=MS.gene016334.t1.exon1;Parent=MS.gene016334.t1 |
| chr8.2 | CDS | 56562403 | 56562421 | 56562403 | ID=cds.MS.gene016334.t1;Parent=MS.gene016334.t1 |
| chr8.2 | exon | 56562050 | 56562148 | 56562050 | ID=MS.gene016334.t1.exon2;Parent=MS.gene016334.t1 |
| chr8.2 | CDS | 56562050 | 56562148 | 56562050 | ID=cds.MS.gene016334.t1;Parent=MS.gene016334.t1 |
| chr8.2 | exon | 56556559 | 56557646 | 56556559 | ID=MS.gene016334.t1.exon3;Parent=MS.gene016334.t1 |
| chr8.2 | CDS | 56556559 | 56557646 | 56556559 | ID=cds.MS.gene016334.t1;Parent=MS.gene016334.t1 |
| chr8.2 | exon | 56556214 | 56556475 | 56556214 | ID=MS.gene016334.t1.exon4;Parent=MS.gene016334.t1 |
| chr8.2 | CDS | 56556214 | 56556475 | 56556214 | ID=cds.MS.gene016334.t1;Parent=MS.gene016334.t1 |
| chr8.2 | exon | 56555519 | 56555565 | 56555519 | ID=MS.gene016334.t1.exon5;Parent=MS.gene016334.t1 |
| chr8.2 | CDS | 56555519 | 56555565 | 56555519 | ID=cds.MS.gene016334.t1;Parent=MS.gene016334.t1 |
| Gene Sequence |
| Protein sequence |
