AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01668

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01668.t1 MTR_2g015500 96.708 243 8 0 1 243 1 243 3.08e-180 494
MS.gene01668.t1 MTR_2g015510 84.298 242 36 1 2 243 3 242 3.81e-156 433
MS.gene01668.t1 MTR_2g015480 67.249 229 58 4 12 239 65 277 2.09e-98 290
MS.gene01668.t1 MTR_4g105680 45.249 221 112 2 25 242 51 265 5.05e-58 185
MS.gene01668.t1 MTR_7g068550 41.981 212 113 3 4 215 6 207 4.49e-54 175
MS.gene01668.t1 MTR_4g016510 38.136 236 134 4 4 235 6 233 1.09e-51 169
MS.gene01668.t1 MTR_7g068530 38.075 239 134 4 4 238 6 234 1.80e-50 165
MS.gene01668.t1 MTR_7g068460 38.318 214 122 4 2 215 31 234 1.25e-47 159
MS.gene01668.t1 MTR_7g068500 37.850 214 123 4 2 215 30 233 6.79e-46 154
MS.gene01668.t1 MTR_7g068470 36.620 213 123 6 4 215 2 203 8.21e-44 148
MS.gene01668.t1 MTR_7g068450 34.694 245 140 5 4 239 2 235 1.45e-43 148
MS.gene01668.t1 MTR_7g068470 37.089 213 118 7 4 215 2 199 2.38e-43 147
MS.gene01668.t1 MTR_3g070460 36.449 214 124 5 4 215 6 209 1.60e-42 145
MS.gene01668.t1 MTR_4g016500 32.484 157 65 5 33 189 277 392 4.11e-18 83.2
MS.gene01668.t1 MTR_7g068520 50.847 59 28 1 4 62 3 60 2.28e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01668.t1 AT5G45920 68.880 241 68 2 4 242 3 238 2.57e-122 348
MS.gene01668.t1 AT5G45920 68.880 241 68 2 4 242 3 238 2.57e-122 348
MS.gene01668.t1 AT5G45920 72.043 186 45 2 4 187 3 183 1.90e-96 280
MS.gene01668.t1 AT5G45920 72.043 186 45 2 4 187 3 183 1.90e-96 280
MS.gene01668.t1 AT5G62930 52.066 242 107 2 4 242 2 237 2.21e-83 249
MS.gene01668.t1 AT5G62930 52.066 242 107 2 4 242 28 263 7.88e-83 248
MS.gene01668.t1 AT5G62930 49.438 178 87 1 68 242 2 179 8.00e-54 172
MS.gene01668.t1 AT2G38180 38.627 233 129 4 4 232 6 228 9.91e-48 160
MS.gene01668.t1 AT3G11210 35.246 244 139 6 4 241 6 236 1.57e-45 153

Find 67 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTACAGAAATGCCTTTA+AGG 0.118994 2.4:+69513908 None:intergenic
GCAGCTTCATTAGTCCTTTC+AGG 0.209931 2.4:+69514436 None:intergenic
TGACCCTAATGACCCCTTAA+AGG 0.261070 2.4:-69513922 MS.gene01668:CDS
GTCAGGGAACTGTTGCATTT+TGG 0.294369 2.4:+69514345 None:intergenic
CTCCATTGTCTCCTTCTTCA+AGG 0.304012 2.4:-69515732 MS.gene01668:intron
AATGCCTTTAAGGGGTCATT+AGG 0.307170 2.4:+69513918 None:intergenic
TCCTTTGATGTTGGAGGATG+GGG 0.334235 2.4:-69516497 MS.gene01668:CDS
TACTACAGAAATGCCTTTAA+GGG 0.399937 2.4:+69513909 None:intergenic
CAAAGCAATCGGAGCAGTTT+CGG 0.402911 2.4:+69515847 None:intergenic
ATGCCTTTAAGGGGTCATTA+GGG 0.414468 2.4:+69513919 None:intergenic
GTCCTTTCAGGAAGACCCTC+TGG 0.415276 2.4:+69514448 None:intergenic
CTGTCACCGCCCTGTGACAC+AGG 0.417724 2.4:+69515875 None:intergenic
CCCTCTGGATTATTCTCAAA+TGG 0.419053 2.4:+69514463 None:intergenic
TTCTTTCAAACATGACTAAG+AGG 0.439390 2.4:-69516559 None:intergenic
ATATTCCTTACATTGATCTC+TGG 0.444800 2.4:-69514369 MS.gene01668:CDS
GAGTCGTGCAACTTCATCAA+TGG 0.457432 2.4:+69514622 None:intergenic
TTGAAAAGCAGAGCATCTAC+TGG 0.476943 2.4:+69515790 None:intergenic
ATCCTTTGATGTTGGAGGAT+GGG 0.480890 2.4:-69516498 MS.gene01668:CDS
AAATGCAACAGTTCCCTGAC+TGG 0.492869 2.4:-69514342 MS.gene01668:CDS
ATGAGGATGACTTTACTTGT+CGG 0.494758 2.4:+69514656 None:intergenic
AGTCGTGCAACTTCATCAAT+GGG 0.507323 2.4:+69514623 None:intergenic
AAGAAAACAGTCAAAGCAAT+CGG 0.513354 2.4:+69515836 None:intergenic
CAGGCAGATGTGGTGCTAAG+AGG 0.513358 2.4:-69515950 MS.gene01668:CDS
ACTGAGGAATCCTTTGATGT+TGG 0.516117 2.4:-69516506 MS.gene01668:CDS
AGGCAGATGTGGTGCTAAGA+GGG 0.519656 2.4:-69515949 MS.gene01668:CDS
CAACACGAGGTGGGCACTGA+AGG 0.520803 2.4:-69515915 MS.gene01668:CDS
TTGATGAAGTTGCACGACTC+AGG 0.521821 2.4:-69514619 MS.gene01668:intron
CAATGGGAGGAGGAGTTATG+AGG 0.526221 2.4:+69514639 None:intergenic
AATCCTTTGATGTTGGAGGA+TGG 0.530232 2.4:-69516499 MS.gene01668:CDS
TTGGTCCAGAGATCAATGTA+AGG 0.532044 2.4:+69514364 None:intergenic
GAGGAATCCTTTGATGTTGG+AGG 0.547556 2.4:-69516503 MS.gene01668:CDS
TTCTCCTTGTATTCATGTAG+AGG 0.550295 2.4:+69515761 None:intergenic
ATCCAGAGGGTCTTCCTGAA+AGG 0.553423 2.4:-69514450 MS.gene01668:CDS
GTGTCACAGGGCGGTGACAG+TGG 0.555563 2.4:-69515872 MS.gene01668:CDS
GTGGTGCTAAGAGGGTATAG+TGG 0.556252 2.4:-69515941 MS.gene01668:CDS
TAGGGTCAATATCAGATACA+AGG 0.556300 2.4:+69513937 None:intergenic
CCTTTGAATTTCAGAAGCGA+TGG 0.564667 2.4:-69514679 MS.gene01668:intron
ACCCCATCCTCCAACATCAA+AGG 0.571101 2.4:+69516496 None:intergenic
TCTCCTTGTATTCATGTAGA+GGG 0.573844 2.4:+69515762 None:intergenic
TGCCAACTATTTCTCACGCA+CGG 0.583852 2.4:-69516465 MS.gene01668:intron
TACCGTGCGTGAGAAATAGT+TGG 0.585214 2.4:+69516463 None:intergenic
TGTCCCTCTACATGAATACA+AGG 0.587200 2.4:-69515765 MS.gene01668:CDS
GGTATGGGTTACCTTGAAGA+AGG 0.588405 2.4:+69515721 None:intergenic
TGGTGCTAAGAGGGTATAGT+GGG 0.591667 2.4:-69515940 MS.gene01668:CDS
GAAAGGACTAATGAAGCTGC+TGG 0.598460 2.4:-69514433 MS.gene01668:CDS
GACGGTTTGCATCTAACTAA+CGG 0.606195 2.4:-69514044 MS.gene01668:CDS
GCAACTTCATCAATGGGAGG+AGG 0.607044 2.4:+69514629 None:intergenic
ACTACAGAAATGCCTTTAAG+GGG 0.608956 2.4:+69513910 None:intergenic
TGAAAAGCAGAGCATCTACT+GGG 0.610569 2.4:+69515791 None:intergenic
TACCTTGAAGAAGGAGACAA+TGG 0.615115 2.4:+69515730 None:intergenic
AGGGTCAATATCAGATACAA+GGG 0.620846 2.4:+69513938 None:intergenic
AGAAGGAGACAATGGAGCAA+AGG 0.621194 2.4:+69515738 None:intergenic
GAGGTGGGCACTGAAGGTGT+TGG 0.628300 2.4:-69515909 MS.gene01668:CDS
GTAAGGAATATGACATTCAG+TGG 0.633665 2.4:+69514381 None:intergenic
CCATTTGAGAATAATCCAGA+GGG 0.637647 2.4:-69514463 MS.gene01668:CDS
TGTCACCGCCCTGTGACACA+GGG 0.639199 2.4:+69515876 None:intergenic
TGCTAGAGCATGCATTGCTG+TGG 0.657710 2.4:-69514404 MS.gene01668:CDS
GGGTCAATATCAGATACAAG+GGG 0.659677 2.4:+69513939 None:intergenic
TAACATGTAACAGGCAGATG+TGG 0.662178 2.4:-69515960 MS.gene01668:intron
GTTTGCATCTAACTAACGGA+GGG 0.673924 2.4:-69514040 MS.gene01668:CDS
TCCATTTGAGAATAATCCAG+AGG 0.677789 2.4:-69514464 MS.gene01668:CDS
CGTGCAACTTCATCAATGGG+AGG 0.679277 2.4:+69514626 None:intergenic
GGTATAGTGGGTACAACACG+AGG 0.696277 2.4:-69515928 MS.gene01668:CDS
TAGTGGGTACAACACGAGGT+GGG 0.715177 2.4:-69515924 MS.gene01668:CDS
GGTTTGCATCTAACTAACGG+AGG 0.720069 2.4:-69514041 MS.gene01668:CDS
ATAGTGGGTACAACACGAGG+TGG 0.732833 2.4:-69515925 MS.gene01668:CDS
GGTCAATATCAGATACAAGG+GGG 0.748357 2.4:+69513940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATCATATTACAATT+TGG + chr2.4:69514154-69514173 None:intergenic 10.0%
!! AATAAATCATATTACAATTT+GGG + chr2.4:69514153-69514172 None:intergenic 10.0%
!!! ATATTTTTAATTTCAATTCT+AGG - chr2.4:69515719-69515738 MS.gene01668:intron 10.0%
!! ATATCATTATTTAATGCATA+TGG - chr2.4:69516348-69516367 MS.gene01668:intron 15.0%
!! GAATAAAATTAGAGTTTAAT+GGG - chr2.4:69514773-69514792 MS.gene01668:intron 15.0%
!! TATAAACAAAACATATAATG+TGG + chr2.4:69514093-69514112 None:intergenic 15.0%
!! TATGATTTGATATGAATATA+CGG - chr2.4:69516371-69516390 MS.gene01668:intron 15.0%
!! TGAATAAAATTAGAGTTTAA+TGG - chr2.4:69514772-69514791 MS.gene01668:intron 15.0%
!! TTAAACATATAATTCTATAG+AGG - chr2.4:69515931-69515950 MS.gene01668:CDS 15.0%
!! AAAATCTAAAAACAGAATGT+GGG + chr2.4:69514409-69514428 None:intergenic 20.0%
!! AATTATACCATTTATACAAG+AGG + chr2.4:69515745-69515764 None:intergenic 20.0%
!! ACTGGAAAAAAGTTTATTTA+AGG - chr2.4:69516135-69516154 MS.gene01668:intron 20.0%
!! TAAAATCTAAAAACAGAATG+TGG + chr2.4:69514410-69514429 None:intergenic 20.0%
!! TATACCTACATATACATTTA+AGG + chr2.4:69515684-69515703 None:intergenic 20.0%
!! TTATCCTTAAATGTATATGT+AGG - chr2.4:69515677-69515696 MS.gene01668:intron 20.0%
!!! AAGTTTGAATCTTTTTGTAA+TGG - chr2.4:69514435-69514454 MS.gene01668:CDS 20.0%
!!! AATTTTATTCAAACTCTGTA+CGG + chr2.4:69514763-69514782 None:intergenic 20.0%
!!! AGTTTGAATCTTTTTGTAAT+GGG - chr2.4:69514436-69514455 MS.gene01668:CDS 20.0%
! AAAGAACAATGATCCTTTAT+TGG + chr2.4:69514857-69514876 None:intergenic 25.0%
! AAGAACAATGATCCTTTATT+GGG + chr2.4:69514856-69514875 None:intergenic 25.0%
! AATTATGTTATCAGTTGGTT+TGG - chr2.4:69515460-69515479 MS.gene01668:intron 25.0%
! AATTTGGAGGTTAATACAAT+AGG - chr2.4:69515202-69515221 MS.gene01668:intron 25.0%
! ATCTTTGATCCTTAAAGAAA+GGG + chr2.4:69515567-69515586 None:intergenic 25.0%
! TATCTTTGATCCTTAAAGAA+AGG + chr2.4:69515568-69515587 None:intergenic 25.0%
! TATTATTGATCATACAGTAG+TGG + chr2.4:69515257-69515276 None:intergenic 25.0%
! TCACCAAAAAGATATATTTG+TGG + chr2.4:69513926-69513945 None:intergenic 25.0%
! TCTGCTTATAACATTCTTAA+AGG - chr2.4:69515588-69515607 MS.gene01668:intron 25.0%
! TTATAATGTTTGTAAAGCTG+TGG - chr2.4:69515117-69515136 MS.gene01668:intron 25.0%
!! ATTTTATTCAAACTCTGTAC+GGG + chr2.4:69514762-69514781 None:intergenic 25.0%
!! GTTTGAATCTTTTTGTAATG+GGG - chr2.4:69514437-69514456 MS.gene01668:CDS 25.0%
!! TTTACCTTCTTTGTTTATCT+TGG - chr2.4:69516170-69516189 MS.gene01668:intron 25.0%
!!! AAAATAGTTTTGATCTCTTG+TGG - chr2.4:69514911-69514930 MS.gene01668:intron 25.0%
!!! AAATAAACTTTTTTCCAGTC+AGG + chr2.4:69516134-69516153 None:intergenic 25.0%
!!! AATAAACTTTTTTCCAGTCA+GGG + chr2.4:69516133-69516152 None:intergenic 25.0%
!!! TCTTTTGAAAAGGATAAATG+AGG - chr2.4:69514468-69514487 MS.gene01668:CDS 25.0%
AAAGCAAAACAAAGCAAGAA+TGG + chr2.4:69514026-69514045 None:intergenic 30.0%
AAAGGATCATTGTTCTTTAG+TGG - chr2.4:69514859-69514878 MS.gene01668:intron 30.0%
AACTGATAACATAATTGCAC+AGG + chr2.4:69515456-69515475 None:intergenic 30.0%
AAGAAAACAGTCAAAGCAAT+CGG + chr2.4:69514626-69514645 None:intergenic 30.0%
ACAATAGGTGATGTTAAATG+TGG - chr2.4:69515217-69515236 MS.gene01668:intron 30.0%
ATAAAAAAGCTGAGAGATGA+AGG - chr2.4:69516466-69516485 MS.gene01668:CDS 30.0%
ATATTCCTTACATTGATCTC+TGG - chr2.4:69516090-69516109 MS.gene01668:intron 30.0%
ATGAACATTGATCAACCTAT+GGG + chr2.4:69515638-69515657 None:intergenic 30.0%
CACATTCTAATAATGTTGCA+TGG - chr2.4:69515040-69515059 MS.gene01668:intron 30.0%
CATAACTCAAGTCACATATA+TGG + chr2.4:69516291-69516310 None:intergenic 30.0%
GTTGACATTGAGTTTATAAG+TGG - chr2.4:69514202-69514221 MS.gene01668:intron 30.0%
GTTTGTTTGTAACATGTAAC+AGG - chr2.4:69514490-69514509 MS.gene01668:intron 30.0%
TAAGCCAAGATAAACAAAGA+AGG + chr2.4:69516177-69516196 None:intergenic 30.0%
TGTGCAATTATGTTATCAGT+TGG - chr2.4:69515455-69515474 MS.gene01668:intron 30.0%
! AGGCCACAAATATATCTTTT+TGG - chr2.4:69513920-69513939 MS.gene01668:CDS 30.0%
! ATTGCTTTGACTGTTTTCTT+TGG - chr2.4:69514626-69514645 MS.gene01668:CDS 30.0%
! TTGCTTTGACTGTTTTCTTT+GGG - chr2.4:69514627-69514646 MS.gene01668:CDS 30.0%
! TTTATTAGTCTCATCGTGTT+AGG - chr2.4:69514122-69514141 MS.gene01668:intron 30.0%
!! GGTTTGTGTTTCTTTTGAAA+AGG - chr2.4:69514458-69514477 MS.gene01668:CDS 30.0%
!!! AAGTTTTTTTTACACTTGCG+TGG - chr2.4:69515612-69515631 MS.gene01668:intron 30.0%
AAGAATGGTCCCTTTCTTTA+AGG - chr2.4:69515555-69515574 MS.gene01668:intron 35.0%
AAGCATGGAATCAAACTCTT+TGG + chr2.4:69515286-69515305 None:intergenic 35.0%
ACTGTGTTGAAACCCAATAA+AGG - chr2.4:69514841-69514860 MS.gene01668:intron 35.0%
AGGGTCAATATCAGATACAA+GGG + chr2.4:69516524-69516543 None:intergenic 35.0%
AGTAATGGCACAAAGAAAGA+AGG + chr2.4:69515428-69515447 None:intergenic 35.0%
AGTGTCTGATCTTGATCAAA+CGG - chr2.4:69514320-69514339 MS.gene01668:intron 35.0%
ATGAGGATGACTTTACTTGT+CGG + chr2.4:69515806-69515825 None:intergenic 35.0%
ATTCATTGGAATCACGCATT+CGG - chr2.4:69514243-69514262 MS.gene01668:intron 35.0%
CATGAACATTGATCAACCTA+TGG + chr2.4:69515639-69515658 None:intergenic 35.0%
CCATTTGAGAATAATCCAGA+GGG - chr2.4:69515996-69516015 MS.gene01668:intron 35.0%
GAGTTTATAAGTGGTGAAAC+TGG - chr2.4:69514211-69514230 MS.gene01668:intron 35.0%
GTAAGGAATATGACATTCAG+TGG + chr2.4:69516081-69516100 None:intergenic 35.0%
TAGGGTCAATATCAGATACA+AGG + chr2.4:69516525-69516544 None:intergenic 35.0%
TCATGTGAGCTGTTAAGTAT+AGG - chr2.4:69515485-69515504 MS.gene01668:intron 35.0%
TCCATTTGAGAATAATCCAG+AGG - chr2.4:69515995-69516014 MS.gene01668:intron 35.0%
TCTCCTTGTATTCATGTAGA+GGG + chr2.4:69514700-69514719 None:intergenic 35.0%
TGGATTTGATAAGTGTGCTT+TGG - chr2.4:69514931-69514950 MS.gene01668:intron 35.0%
TTCTAGGCCTCTTGTATAAA+TGG - chr2.4:69515735-69515754 MS.gene01668:CDS 35.0%
TTCTCCTTGTATTCATGTAG+AGG + chr2.4:69514701-69514720 None:intergenic 35.0%
! AGAAAGAAGGCATGATGTTT+AGG + chr2.4:69515415-69515434 None:intergenic 35.0%
! TCTTTCTTTGTGCCATTACT+TGG - chr2.4:69515428-69515447 MS.gene01668:intron 35.0%
! TGCTTTGACTGTTTTCTTTG+GGG - chr2.4:69514628-69514647 MS.gene01668:CDS 35.0%
! TTGATTCCATGCTTTTCTGT+GGG - chr2.4:69515292-69515311 MS.gene01668:intron 35.0%
! TTTGATTCCATGCTTTTCTG+TGG - chr2.4:69515291-69515310 MS.gene01668:intron 35.0%
!! CGACTCAGGTATTGTTTTAT+TGG - chr2.4:69515854-69515873 MS.gene01668:CDS 35.0%
AATCCTTTGATGTTGGAGGA+TGG - chr2.4:69513960-69513979 MS.gene01668:CDS 40.0%
AATGGGTATGCACACAATGA+GGG - chr2.4:69514790-69514809 MS.gene01668:intron 40.0%
ACTGAGGAATCCTTTGATGT+TGG - chr2.4:69513953-69513972 MS.gene01668:CDS 40.0%
ACTGGATTCCATGCATTCAT+TGG - chr2.4:69514229-69514248 MS.gene01668:intron 40.0%
AGTCGTGCAACTTCATCAAT+GGG + chr2.4:69515839-69515858 None:intergenic 40.0%
ATAGGCATGCATGTTAGCAA+TGG - chr2.4:69515503-69515522 MS.gene01668:intron 40.0%
ATCCTTTGATGTTGGAGGAT+GGG - chr2.4:69513961-69513980 MS.gene01668:CDS 40.0%
ATTCAAACTCTGTACGGGTA+TGG + chr2.4:69514757-69514776 None:intergenic 40.0%
ATTGCACAGGAACCAAGTAA+TGG + chr2.4:69515443-69515462 None:intergenic 40.0%
CAAATTCCTGAAATCCCACA+GGG + chr2.4:69515188-69515207 None:intergenic 40.0%
CCCTCTGGATTATTCTCAAA+TGG + chr2.4:69515999-69516018 None:intergenic 40.0%
CCTTTGAATTTCAGAAGCGA+TGG - chr2.4:69515780-69515799 MS.gene01668:CDS 40.0%
CTCAAATTGTCGACCACTTA+TGG + chr2.4:69514376-69514395 None:intergenic 40.0%
GACGGTTTGCATCTAACTAA+CGG - chr2.4:69516415-69516434 MS.gene01668:intron 40.0%
GAGAACGAAAAGTTTCTCTC+TGG - chr2.4:69515092-69515111 MS.gene01668:intron 40.0%
GGAAAACCAACCTCTACTTT+AGG - chr2.4:69515527-69515546 MS.gene01668:intron 40.0%
GGGTCAATATCAGATACAAG+GGG + chr2.4:69516523-69516542 None:intergenic 40.0%
GGTCAATATCAGATACAAGG+GGG + chr2.4:69516522-69516541 None:intergenic 40.0%
GTACGAGATGCTTGTGTAAA+AGG - chr2.4:69515325-69515344 MS.gene01668:intron 40.0%
GTGATGTTAAATGTGGACTC+TGG - chr2.4:69515224-69515243 MS.gene01668:intron 40.0%
GTTTGCATCTAACTAACGGA+GGG - chr2.4:69516419-69516438 MS.gene01668:intron 40.0%
TAACATGTAACAGGCAGATG+TGG - chr2.4:69514499-69514518 MS.gene01668:intron 40.0%
TACCTTGAAGAAGGAGACAA+TGG + chr2.4:69514732-69514751 None:intergenic 40.0%
TAGGCATGCATGTTAGCAAT+GGG - chr2.4:69515504-69515523 MS.gene01668:intron 40.0%
TGTCCCTCTACATGAATACA+AGG - chr2.4:69514694-69514713 MS.gene01668:intron 40.0%
TGTTTACCGAAATGCAGTGA+CGG - chr2.4:69516397-69516416 MS.gene01668:intron 40.0%
TTCAAACTCTGTACGGGTAT+GGG + chr2.4:69514756-69514775 None:intergenic 40.0%
TTCATTGGAATCACGCATTC+GGG - chr2.4:69514244-69514263 MS.gene01668:intron 40.0%
TTCTTCCACTCCTAAAGTAG+AGG + chr2.4:69515540-69515559 None:intergenic 40.0%
TTGGTCCAGAGATCAATGTA+AGG + chr2.4:69516098-69516117 None:intergenic 40.0%
TTTGGTCGCGTATATATTGC+AGG - chr2.4:69514949-69514968 MS.gene01668:intron 40.0%
! AATGCCTTTAAGGGGTCATT+AGG + chr2.4:69516544-69516563 None:intergenic 40.0%
! ATGCCTTTAAGGGGTCATTA+GGG + chr2.4:69516543-69516562 None:intergenic 40.0%
! CTACTTTAGGAGTGGAAGAA+TGG - chr2.4:69515540-69515559 MS.gene01668:intron 40.0%
! TAATGGGTATGCACACAATG+AGG - chr2.4:69514789-69514808 MS.gene01668:intron 40.0%
! TGAAAAGCAGAGCATCTACT+GGG + chr2.4:69514671-69514690 None:intergenic 40.0%
! TTGAAAAGCAGAGCATCTAC+TGG + chr2.4:69514672-69514691 None:intergenic 40.0%
!! CCATCGCTTCTGAAATTCAA+AGG + chr2.4:69515783-69515802 None:intergenic 40.0%
!! TTTTGGTGACTCAATCACTG+AGG - chr2.4:69513937-69513956 MS.gene01668:CDS 40.0%
AAATGCAACAGTTCCCTGAC+TGG - chr2.4:69516117-69516136 MS.gene01668:intron 45.0%
AATACCTGAATGCGAAGAGC+CGG - chr2.4:69514348-69514367 MS.gene01668:CDS 45.0%
ACGGCTATAGTGTAGGACTA+GGG - chr2.4:69515148-69515167 MS.gene01668:intron 45.0%
AGAAGGAGACAATGGAGCAA+AGG + chr2.4:69514724-69514743 None:intergenic 45.0%
AGGCATGCATGTTAGCAATG+GGG - chr2.4:69515505-69515524 MS.gene01668:intron 45.0%
CAAAGCAATCGGAGCAGTTT+CGG + chr2.4:69514615-69514634 None:intergenic 45.0%
CCAAATTCCTGAAATCCCAC+AGG + chr2.4:69515189-69515208 None:intergenic 45.0%
CCTGTGGGATTTCAGGAATT+TGG - chr2.4:69515186-69515205 MS.gene01668:intron 45.0%
CTCCATTGTCTCCTTCTTCA+AGG - chr2.4:69514727-69514746 MS.gene01668:intron 45.0%
GAAAGGACTAATGAAGCTGC+TGG - chr2.4:69516026-69516045 MS.gene01668:intron 45.0%
GAGGAATCCTTTGATGTTGG+AGG - chr2.4:69513956-69513975 MS.gene01668:CDS 45.0%
GAGTCGTGCAACTTCATCAA+TGG + chr2.4:69515840-69515859 None:intergenic 45.0%
GCAGCTTCATTAGTCCTTTC+AGG + chr2.4:69516026-69516045 None:intergenic 45.0%
GCGTGATTCCAATGAATGCA+TGG + chr2.4:69514240-69514259 None:intergenic 45.0%
GGTTTGCATCTAACTAACGG+AGG - chr2.4:69516418-69516437 MS.gene01668:intron 45.0%
GTATGCACACAATGAGGGAT+TGG - chr2.4:69514795-69514814 MS.gene01668:intron 45.0%
GTGGGATTTCAGGAATTTGG+AGG - chr2.4:69515189-69515208 MS.gene01668:intron 45.0%
TACCGTGCGTGAGAAATAGT+TGG + chr2.4:69513999-69514018 None:intergenic 45.0%
TCCACTCCTAAAGTAGAGGT+TGG + chr2.4:69515536-69515555 None:intergenic 45.0%
TCCATTCCCACAGAAAAGCA+TGG + chr2.4:69515301-69515320 None:intergenic 45.0%
TCCTTTGATGTTGGAGGATG+GGG - chr2.4:69513962-69513981 MS.gene01668:CDS 45.0%
TGACCCTAATGACCCCTTAA+AGG - chr2.4:69516537-69516556 MS.gene01668:CDS 45.0%
TGCAAACCGTCACTGCATTT+CGG + chr2.4:69516406-69516425 None:intergenic 45.0%
TTGATGAAGTTGCACGACTC+AGG - chr2.4:69515840-69515859 MS.gene01668:CDS 45.0%
TTGTCGACCACTTATGGATC+CGG + chr2.4:69514370-69514389 None:intergenic 45.0%
TTGTTCTTTAGTGGCACAGC+AGG - chr2.4:69514868-69514887 MS.gene01668:intron 45.0%
! ACCAACCTCTACTTTAGGAG+TGG - chr2.4:69515532-69515551 MS.gene01668:intron 45.0%
! AGAGTTTTCCCTGTGTCACA+GGG - chr2.4:69514575-69514594 MS.gene01668:intron 45.0%
! AGGGATTGGCACAGATTATC+AGG - chr2.4:69514809-69514828 MS.gene01668:intron 45.0%
! CTTTTCTGTGGGAATGGAGT+TGG - chr2.4:69515303-69515322 MS.gene01668:intron 45.0%
! GGTATGGGTTACCTTGAAGA+AGG + chr2.4:69514741-69514760 None:intergenic 45.0%
! GTCAGGGAACTGTTGCATTT+TGG + chr2.4:69516117-69516136 None:intergenic 45.0%
! TCCATGCTTTTCTGTGGGAA+TGG - chr2.4:69515297-69515316 MS.gene01668:intron 45.0%
! TGCCAACTATTTCTCACGCA+CGG - chr2.4:69513994-69514013 MS.gene01668:CDS 45.0%
! TGGTGCTAAGAGGGTATAGT+GGG - chr2.4:69514519-69514538 MS.gene01668:intron 45.0%
!! TCTTCGCATTCAGGTATTGG+TGG + chr2.4:69514346-69514365 None:intergenic 45.0%
!! AATAAAAAAAATAAAAAAAG+TGG + chr2.4:69515352-69515371 None:intergenic 5.0%
!!! AATAATAATTATTTTTCATT+TGG - chr2.4:69514979-69514998 MS.gene01668:intron 5.0%
ACAGTGCACGGCTATAGTGT+AGG - chr2.4:69515141-69515160 MS.gene01668:intron 50.0%
ACCCCATCCTCCAACATCAA+AGG + chr2.4:69513966-69513985 None:intergenic 50.0%
ATCCAGAGGGTCTTCCTGAA+AGG - chr2.4:69516009-69516028 MS.gene01668:intron 50.0%
CAATGGGAGGAGGAGTTATG+AGG + chr2.4:69515823-69515842 None:intergenic 50.0%
CACGGCTATAGTGTAGGACT+AGG - chr2.4:69515147-69515166 MS.gene01668:intron 50.0%
CGTGCAACTTCATCAATGGG+AGG + chr2.4:69515836-69515855 None:intergenic 50.0%
GATACAAGGGGGAGATCAAC+TGG + chr2.4:69516511-69516530 None:intergenic 50.0%
GCAACTTCATCAATGGGAGG+AGG + chr2.4:69515833-69515852 None:intergenic 50.0%
GGCATGCATGTTAGCAATGG+GGG - chr2.4:69515506-69515525 MS.gene01668:intron 50.0%
GGCTCTTCGCATTCAGGTAT+TGG + chr2.4:69514349-69514368 None:intergenic 50.0%
TAAAGCTGTGGCACAGTGCA+CGG - chr2.4:69515129-69515148 MS.gene01668:intron 50.0%
TGCTAGAGCATGCATTGCTG+TGG - chr2.4:69516055-69516074 MS.gene01668:intron 50.0%
TTCTTTAGTGGCACAGCAGG+AGG - chr2.4:69514871-69514890 MS.gene01668:intron 50.0%
TTTACACTTGCGTGGCCCAT+AGG - chr2.4:69515620-69515639 MS.gene01668:intron 50.0%
! AGGCAGATGTGGTGCTAAGA+GGG - chr2.4:69514510-69514529 MS.gene01668:intron 50.0%
! CAGAGTTTTCCCTGTGTCAC+AGG - chr2.4:69514574-69514593 MS.gene01668:intron 50.0%
! GTGGTGCTAAGAGGGTATAG+TGG - chr2.4:69514518-69514537 MS.gene01668:intron 50.0%
! TAGTGGGTACAACACGAGGT+GGG - chr2.4:69514535-69514554 MS.gene01668:intron 50.0%
!! ATAGTGGGTACAACACGAGG+TGG - chr2.4:69514534-69514553 MS.gene01668:intron 50.0%
!! GGTATAGTGGGTACAACACG+AGG - chr2.4:69514531-69514550 MS.gene01668:intron 50.0%
ACATGGCCCTGTGGGATTTC+AGG - chr2.4:69515179-69515198 MS.gene01668:intron 55.0%
CGAAGAGCCGGATCCATAAG+TGG - chr2.4:69514360-69514379 MS.gene01668:CDS 55.0%
GTCCTTTCAGGAAGACCCTC+TGG + chr2.4:69516014-69516033 None:intergenic 55.0%
! CAGGCAGATGTGGTGCTAAG+AGG - chr2.4:69514509-69514528 MS.gene01668:intron 55.0%
! GTTTTCCCTGTGTCACAGGG+CGG - chr2.4:69514578-69514597 MS.gene01668:intron 55.0%
ATGCACGCACATGGCCCTGT+GGG - chr2.4:69515171-69515190 MS.gene01668:intron 60.0%
GGACTAGGGATGCACGCACA+TGG - chr2.4:69515162-69515181 MS.gene01668:intron 60.0%
GGATCCGGCTCTTCGCATTC+AGG + chr2.4:69514355-69514374 None:intergenic 60.0%
TGTCACCGCCCTGTGACACA+GGG + chr2.4:69514586-69514605 None:intergenic 60.0%
!! CAACACGAGGTGGGCACTGA+AGG - chr2.4:69514544-69514563 MS.gene01668:intron 60.0%
!! GAGGTGGGCACTGAAGGTGT+TGG - chr2.4:69514550-69514569 MS.gene01668:intron 60.0%
CTGTCACCGCCCTGTGACAC+AGG + chr2.4:69514587-69514606 None:intergenic 65.0%
GATGCACGCACATGGCCCTG+TGG - chr2.4:69515170-69515189 MS.gene01668:intron 65.0%
GTGTCACAGGGCGGTGACAG+TGG - chr2.4:69514587-69514606 MS.gene01668:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69513911 69516570 69513911 ID=MS.gene01668
chr2.4 mRNA 69513911 69516570 69513911 ID=MS.gene01668.t1;Parent=MS.gene01668
chr2.4 exon 69516466 69516570 69516466 ID=MS.gene01668.t1.exon1;Parent=MS.gene01668.t1
chr2.4 CDS 69516466 69516570 69516466 ID=cds.MS.gene01668.t1;Parent=MS.gene01668.t1
chr2.4 exon 69515733 69515969 69515733 ID=MS.gene01668.t1.exon2;Parent=MS.gene01668.t1
chr2.4 CDS 69515733 69515969 69515733 ID=cds.MS.gene01668.t1;Parent=MS.gene01668.t1
chr2.4 exon 69514620 69514687 69514620 ID=MS.gene01668.t1.exon3;Parent=MS.gene01668.t1
chr2.4 CDS 69514620 69514687 69514620 ID=cds.MS.gene01668.t1;Parent=MS.gene01668.t1
chr2.4 exon 69514325 69514489 69514325 ID=MS.gene01668.t1.exon4;Parent=MS.gene01668.t1
chr2.4 CDS 69514325 69514489 69514325 ID=cds.MS.gene01668.t1;Parent=MS.gene01668.t1
chr2.4 exon 69513911 69514067 69513911 ID=MS.gene01668.t1.exon5;Parent=MS.gene01668.t1
chr2.4 CDS 69513911 69514067 69513911 ID=cds.MS.gene01668.t1;Parent=MS.gene01668.t1
Gene Sequence

>MS.gene01668

ATGACTAAGAGGCCACAAATATATCTTTTTGGTGACTCAATCACTGAGGAATCCTTTGATGTTGGAGGATGGGGTGCTTCTCTTGCCAACTATTTCTCACGCACGGTAACATCCATTCTTGCTTTGTTTTGCTTTAACAACTCATATTTTCATATCTGATTCATGTTCTTGTTAACATTCCACATTATATGTTTTGTTTATAAATGATTTATTTATTAGTCTCATCGTGTTAGGTGCAACCCAAATTGTAATATGATTTATTTATTAGTCTCGTCGTGTTGAGAATCTCACGTTGACATTGAGTTTATAAGTGGTGAAACTGGATTCCATGCATTCATTGGAATCACGCATTCGGGACATTGTTGATCGTTCTAAATTTTTTATTTCAAAATATAGAAATTGAATCTAAAGTGTCTGATCTTGATCAAACGGCCACCAATACCTGAATGCGAAGAGCCGGATCCATAAGTGGTCGACAATTTGAGTTAATTGTATCCCACATTCTGTTTTTAGATTTTAATGTAAAGTTTGAATCTTTTTGTAATGGGGTTTGTGTTTCTTTTGAAAAGGATAAATGAGGTTTGTTTGTAACATGTAACAGGCAGATGTGGTGCTAAGAGGGTATAGTGGGTACAACACGAGGTGGGCACTGAAGGTGTTGGACAGAGTTTTCCCTGTGTCACAGGGCGGTGACAGTGGAACCGAAACTGCTCCGATTGCTTTGACTGTTTTCTTTGGGGCTAATGACGCTTGTCTTCCCAGTAGATGCTCTGCTTTTCAACATGTCCCTCTACATGAATACAAGGAGAACCTTTGCTCCATTGTCTCCTTCTTCAAGGTAACCCATACCCGTACAGAGTTTGAATAAAATTAGAGTTTAATGGGTATGCACACAATGAGGGATTGGCACAGATTATCAGGTAAAAGACTACTGTGTTGAAACCCAATAAAGGATCATTGTTCTTTAGTGGCACAGCAGGAGGCTTTTTTCTTGAAAAAAAAAATAGTTTTGATCTCTTGTGGATTTGATAAGTGTGCTTTGGTCGCGTATATATTGCAGGTTACAGAAATAATAATTATTTTTCATTTGGAAGTTTGTCGCTGTTATGATTATTTAAGTATAGTCTTGCACATTCTAATAATGTTGCATGGCGCATTAGCTATTGCAACTGTTAGATCTAGAGAACGAAAAGTTTCTCTCTGGATTTATAATGTTTGTAAAGCTGTGGCACAGTGCACGGCTATAGTGTAGGACTAGGGATGCACGCACATGGCCCTGTGGGATTTCAGGAATTTGGAGGTTAATACAATAGGTGATGTTAAATGTGGACTCTGGCTTTTTGCCACTACTGTATGATCAATAATACACACTCCAAAGAGTTTGATTCCATGCTTTTCTGTGGGAATGGAGTTGGTACGAGATGCTTGTGTAAAAGGTCCACTTTTTTTATTTTTTTTATTGAATTGAGTCTAGCATTGTTGTTAGCGTTGTTTTGACAAACCTAAACATCATGCCTTCTTTCTTTGTGCCATTACTTGGTTCCTGTGCAATTATGTTATCAGTTGGTTTGGTCTCATGTGAGCTGTTAAGTATAGGCATGCATGTTAGCAATGGGGGAAAACCAACCTCTACTTTAGGAGTGGAAGAATGGTCCCTTTCTTTAAGGATCAAAGATATCTGCTTATAACATTCTTAAAGGAAAGTTTTTTTTACACTTGCGTGGCCCATAGGTTGATCAATGTTCATGCTTATATTAAGAAAATTGTTATCCTTAAATGTATATGTAGGTATATAAGTTGACGCTTGAATATTTTTAATTTCAATTCTAGGCCTCTTGTATAAATGGTATAATTAGACACTGCAAATTTCCTTTGAATTTCAGAAGCGATGGCCGACAAGTAAAGTCATCCTCATAACTCCTCCTCCCATTGATGAAGTTGCACGACTCAGGTATTGTTTTATTGGCTTTTTATCTTCATTTTGTCACTGATTTTATTATGTTTTCTTTGTTTGTGACATTTAAACATATAATTCTATAGAGGTTAATCACACTTGAAGAATTTCTAAAAATCTATGACAGATATCCATTTGAGAATAATCCAGAGGGTCTTCCTGAAAGGACTAATGAAGCTGCTGGTGAATATGCTAGAGCATGCATTGCTGTGGCCACTGAATGTCATATTCCTTACATTGATCTCTGGACCAAAATGCAACAGTTCCCTGACTGGAAAAAAGTTTATTTAAGGTTTATAATTTATTTTACCTTCTTTGTTTATCTTGGCTTACAACATTTTGCATTTTTGCATCATACATATAAAAGCTTACGAAGTTTTCATTGCTGACTTGATTGATTTTTCAAAATGATCAATTCTGTAGCCATATATGTGACTTGAGTTATGCGAAAATGACTAATTCTGTATGAATTTGCAAAGAATAATATCATTATTTAATGCATATGGTATGATTTGATATGAATATACGGTTTTGTTTACCGAAATGCAGTGACGGTTTGCATCTAACTAACGGAGGGAATCAACTTGTATTTGAAGAAGTAATAAAAAAGCTGAGAGATGAAGGTTTGAGTCTAGAATCTATTCCAGTTGATCTCCCCCTTGTATCTGATATTGACCCTAATGACCCCTTAAAGGCATTTCTGTAG
Protein sequence

>MS.gene01668.t1

MTKRPQIYLFGDSITEESFDVGGWGASLANYFSRTADVVLRGYSGYNTRWALKVLDRVFPVSQGGDSGTETAPIALTVFFGANDACLPSRCSAFQHVPLHEYKENLCSIVSFFKKRWPTSKVILITPPPIDEVARLRYPFENNPEGLPERTNEAAGEYARACIAVATECHIPYIDLWTKMQQFPDWKKVYLSDGLHLTNGGNQLVFEEVIKKLRDEGLSLESIPVDLPLVSDIDPNDPLKAFL