AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020684


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020684.t1 MTR_5g094340 93.765 417 25 1 1 416 1 417 0.0 769
MS.gene020684.t1 MTR_3g055960 73.210 433 96 8 1 416 3 432 0.0 595
MS.gene020684.t1 MTR_5g089720 39.663 416 189 10 53 416 32 437 3.22e-86 269
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020684.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene020684.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene020684.t1 AT5G55530 55.607 428 155 12 1 416 1 405 1.91e-145 420
MS.gene020684.t1 AT1G50570 55.039 387 145 10 39 416 22 388 1.27e-127 374
MS.gene020684.t1 AT1G50570 55.039 387 145 10 39 416 22 388 1.27e-127 374
MS.gene020684.t1 AT5G12300 47.500 200 96 4 54 247 19 215 3.03e-53 182

Find 71 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAATGAAGACCACTTAA+TGG 0.202475 5.4:+74229320 MS.gene020684:CDS
TCCAATCAGGAGCAAATTTC+AGG 0.224053 5.4:+74229549 MS.gene020684:CDS
TTCACACTCACAACAGGTTT+TGG 0.272566 5.4:-74229645 None:intergenic
TAGCATAAACATCTTGCTTA+TGG 0.294667 5.4:-74228852 None:intergenic
TCGAATCCAGAGGAATTTAT+TGG 0.302487 5.4:+74228775 MS.gene020684:CDS
GAAAATCTTAGTTCAGAAAA+AGG 0.307362 5.4:+74229387 MS.gene020684:CDS
CCAAAGCAAAACCAAGTAAT+TGG 0.309892 5.4:-74229044 None:intergenic
TTTCTGTCCAATTCAGTATT+AGG 0.324501 5.4:-74229219 None:intergenic
ATATCCTGCTGCACCATCTT+TGG 0.325154 5.4:-74229681 None:intergenic
TCCTCCTCCATTGATAATCT+TGG 0.335282 5.4:-74228914 None:intergenic
GAAAGTGGACCAACTAGTTC+AGG 0.360152 5.4:+74229777 MS.gene020684:CDS
ACCTGAAATTTGCTCCTGAT+TGG 0.370968 5.4:-74229550 None:intergenic
AGCAATGCCTAATACTGAAT+TGG 0.385474 5.4:+74229212 MS.gene020684:CDS
TGCTGCACCATCTTTGGCTC+TGG 0.397036 5.4:-74229675 None:intergenic
ATAACCTTCGTCTAAGTGTT+AGG 0.404097 5.4:+74228956 MS.gene020684:CDS
GTCAATACCAAGATTATCAA+TGG 0.409373 5.4:+74228907 MS.gene020684:CDS
TCTAAAGACATTGAGGTCAA+TGG 0.413522 5.4:+74228733 MS.gene020684:CDS
TGGCGCTTCACCAGATGTTA+TGG 0.425510 5.4:+74229182 MS.gene020684:CDS
TATCTTGAAGACCAATTACT+TGG 0.432499 5.4:+74229033 MS.gene020684:CDS
TCAATATTCACACTCACAAC+AGG 0.440373 5.4:-74229651 None:intergenic
GATGGTGCAGCAGGATATAG+TGG 0.440448 5.4:+74229686 MS.gene020684:CDS
GTGAGATCTGGATGCTTAGC+AGG 0.442573 5.4:+74229001 MS.gene020684:CDS
CTTCTCTCAAATGTGAGATC+TGG 0.446006 5.4:+74228989 MS.gene020684:CDS
ACCATTCAAGAGTTCTGTCT+TGG 0.461139 5.4:+74228657 MS.gene020684:CDS
TGCTACTAAATCTTCCAATC+AGG 0.468664 5.4:+74229536 MS.gene020684:CDS
TCTGAAGTATTGATGAAAGA+TGG 0.470375 5.4:+74229075 MS.gene020684:CDS
CTCATTCAGCAACGTATTCG+AGG 0.473738 5.4:-74229616 None:intergenic
TGAATCTGTTCAACCTCCTA+AGG 0.483694 5.4:+74229446 MS.gene020684:CDS
GAACAAAACCTGCTGGTGAA+TGG 0.486115 5.4:-74229140 None:intergenic
CTAGGTGGAGATGCAACCTT+AGG 0.492862 5.4:-74229462 None:intergenic
ATACTTTGGTACTGATAGCT+TGG 0.493813 5.4:+74229350 MS.gene020684:CDS
AGAGCCAAAGATGGTGCAGC+AGG 0.494831 5.4:+74229677 MS.gene020684:CDS
ATGTTTACATACATCAAGCT+AGG 0.499383 5.4:+74228806 MS.gene020684:CDS
CCATTCAAGAGTTCTGTCTT+GGG 0.499630 5.4:+74228658 MS.gene020684:CDS
GTTGAGTTTCCTGAACTAGT+TGG 0.500780 5.4:-74229786 None:intergenic
ACTACTCCAATAAATTCCTC+TGG 0.500999 5.4:-74228781 None:intergenic
GATCTATTCCATTCACCAGC+AGG 0.502121 5.4:+74229132 MS.gene020684:CDS
TCCTTTGTCTAAAGACATTG+AGG 0.512174 5.4:+74228726 MS.gene020684:CDS
TGGTGTTGTTTCCTCTTCAG+TGG 0.515128 5.4:+74229491 MS.gene020684:CDS
AAGAGTTCTGTCTTGGGACA+TGG 0.516859 5.4:+74228664 MS.gene020684:CDS
CTTCCTATGGACTTTGAAAG+TGG 0.518348 5.4:+74229762 MS.gene020684:CDS
GCATCTCCACCTAGCAGTAA+TGG 0.534582 5.4:+74229471 MS.gene020684:CDS
TTAATGGTTTCAGAATACTT+TGG 0.535977 5.4:+74229336 MS.gene020684:CDS
AGTATTCTGAAACCATTAAG+TGG 0.538501 5.4:-74229332 None:intergenic
GCTGAAATTGCCATAACATC+TGG 0.541367 5.4:-74229192 None:intergenic
GGTGGAGATGCAACCTTAGG+AGG 0.554345 5.4:-74229459 None:intergenic
ACCAAGATTATCAATGGAGG+AGG 0.555656 5.4:+74228913 MS.gene020684:CDS
AATACTGAATTGGACAGAAA+CGG 0.555790 5.4:+74229222 MS.gene020684:CDS
ACATTTGAGAGAAGAATCAA+TGG 0.559219 5.4:-74228980 None:intergenic
CCCAAGACAGAACTCTTGAA+TGG 0.559287 5.4:-74228658 None:intergenic
TGAGCTTGCAGCCACTGAAG+AGG 0.564334 5.4:-74229502 None:intergenic
CAATTGTCACTTGCTTACAA+TGG 0.566767 5.4:+74229162 MS.gene020684:CDS
AGATTGTAGTTTCTGTTCAG+AGG 0.567533 5.4:-74229808 None:intergenic
GGTCCACTTTCAAAGTCCAT+AGG 0.582924 5.4:-74229765 None:intergenic
AATGGTGACACAACAGATTG+TGG 0.593267 5.4:-74228640 None:intergenic
CGAAGGTTATCGTTAAACAC+GGG 0.594871 5.4:-74228943 None:intergenic
TTCATCAATACTTCAGACAA+AGG 0.596605 5.4:-74229069 None:intergenic
AAAAGAGTTGATCTCAAAGA+CGG 0.598346 5.4:+74229582 MS.gene020684:CDS
ACCTCAATGTCTTTAGACAA+AGG 0.602535 5.4:-74228727 None:intergenic
TATTGAGCCAGAGCCAAAGA+TGG 0.602654 5.4:+74229668 MS.gene020684:CDS
TGCTGGTGAATGGAATAGAT+CGG 0.604836 5.4:-74229130 None:intergenic
TGTTTACATACATCAAGCTA+GGG 0.605167 5.4:+74228807 MS.gene020684:CDS
GACAATTGAACAAAACCTGC+TGG 0.612847 5.4:-74229147 None:intergenic
GAAACAACACCATTACTGCT+AGG 0.628325 5.4:-74229480 None:intergenic
TGGTCCTAACACTTAGACGA+AGG 0.635127 5.4:-74228960 None:intergenic
AATACCAAGATTATCAATGG+AGG 0.656844 5.4:+74228910 MS.gene020684:CDS
ACGAAGGTTATCGTTAAACA+CGG 0.658274 5.4:-74228944 None:intergenic
TGTTGCAGTGTCGAATCCAG+AGG 0.689971 5.4:+74228765 MS.gene020684:CDS
ACAACACCATTACTGCTAGG+TGG 0.692805 5.4:-74229477 None:intergenic
TGAGATCTGGATGCTTAGCA+GGG 0.697112 5.4:+74229002 MS.gene020684:CDS
GAAGGTTATCGTTAAACACG+GGG 0.724753 5.4:-74228942 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTACAATTTTTGGATTT+GGG - chr5.4:74229308-74229327 None:intergenic 20.0%
!!! TCATTTACAATTTTTGGATT+TGG - chr5.4:74229309-74229328 None:intergenic 20.0%
! GAAAATCTTAGTTCAGAAAA+AGG + chr5.4:74229387-74229406 MS.gene020684:CDS 25.0%
! TTAATGGTTTCAGAATACTT+TGG + chr5.4:74229336-74229355 MS.gene020684:CDS 25.0%
!! TGGTCTTCATTTACAATTTT+TGG - chr5.4:74229315-74229334 None:intergenic 25.0%
!!! TTGGTATTGACAAAATTTTC+AGG - chr5.4:74228898-74228917 None:intergenic 25.0%
AATACCAAGATTATCAATGG+AGG + chr5.4:74228910-74228929 MS.gene020684:CDS 30.0%
AATACTGAATTGGACAGAAA+CGG + chr5.4:74229222-74229241 MS.gene020684:CDS 30.0%
ACATTTGAGAGAAGAATCAA+TGG - chr5.4:74228983-74229002 None:intergenic 30.0%
ATGTTTACATACATCAAGCT+AGG + chr5.4:74228806-74228825 MS.gene020684:CDS 30.0%
GTCAATACCAAGATTATCAA+TGG + chr5.4:74228907-74228926 MS.gene020684:CDS 30.0%
TAGCATAAACATCTTGCTTA+TGG - chr5.4:74228855-74228874 None:intergenic 30.0%
TATCTTGAAGACCAATTACT+TGG + chr5.4:74229033-74229052 MS.gene020684:CDS 30.0%
TGTAAATGAAGACCACTTAA+TGG + chr5.4:74229320-74229339 MS.gene020684:CDS 30.0%
TGTTTACATACATCAAGCTA+GGG + chr5.4:74228807-74228826 MS.gene020684:CDS 30.0%
TTCATCAATACTTCAGACAA+AGG - chr5.4:74229072-74229091 None:intergenic 30.0%
TTTCTGTCCAATTCAGTATT+AGG - chr5.4:74229222-74229241 None:intergenic 30.0%
! AAAAGAGTTGATCTCAAAGA+CGG + chr5.4:74229582-74229601 MS.gene020684:CDS 30.0%
! AAGATTTTCAGTATCAGTAG+TGG - chr5.4:74229376-74229395 None:intergenic 30.0%
!! AGTATTCTGAAACCATTAAG+TGG - chr5.4:74229335-74229354 None:intergenic 30.0%
!! CTTCATTTTTGCTAATGACT+CGG - chr5.4:74229742-74229761 None:intergenic 30.0%
!! TCTGAAGTATTGATGAAAGA+TGG + chr5.4:74229075-74229094 MS.gene020684:CDS 30.0%
ACCTCAATGTCTTTAGACAA+AGG - chr5.4:74228730-74228749 None:intergenic 35.0%
ACGAAGGTTATCGTTAAACA+CGG - chr5.4:74228947-74228966 None:intergenic 35.0%
ACTACTCCAATAAATTCCTC+TGG - chr5.4:74228784-74228803 None:intergenic 35.0%
AGATTGTAGTTTCTGTTCAG+AGG - chr5.4:74229811-74229830 None:intergenic 35.0%
AGCAAAAATGAAGCTTCCTA+TGG + chr5.4:74229749-74229768 MS.gene020684:CDS 35.0%
AGCAATGCCTAATACTGAAT+TGG + chr5.4:74229212-74229231 MS.gene020684:CDS 35.0%
ATAACCTTCGTCTAAGTGTT+AGG + chr5.4:74228956-74228975 MS.gene020684:CDS 35.0%
CCAAAGCAAAACCAAGTAAT+TGG - chr5.4:74229047-74229066 None:intergenic 35.0%
TCAATATTCACACTCACAAC+AGG - chr5.4:74229654-74229673 None:intergenic 35.0%
TCCTTTGTCTAAAGACATTG+AGG + chr5.4:74228726-74228745 MS.gene020684:CDS 35.0%
TCGAATCCAGAGGAATTTAT+TGG + chr5.4:74228775-74228794 MS.gene020684:CDS 35.0%
TCTAAAGACATTGAGGTCAA+TGG + chr5.4:74228733-74228752 MS.gene020684:CDS 35.0%
TGCTACTAAATCTTCCAATC+AGG + chr5.4:74229536-74229555 MS.gene020684:CDS 35.0%
! AGTAACTCACGTGTGTTTTA+TGG + chr5.4:74229840-74229859 MS.gene020684:CDS 35.0%
! CAATTGTCACTTGCTTACAA+TGG + chr5.4:74229162-74229181 MS.gene020684:CDS 35.0%
!! AGAAAAAGGTGTTCAACTTG+TGG + chr5.4:74229401-74229420 MS.gene020684:CDS 35.0%
!! ATACTTTGGTACTGATAGCT+TGG + chr5.4:74229350-74229369 MS.gene020684:CDS 35.0%
!!! CCAATTACTTGGTTTTGCTT+TGG + chr5.4:74229044-74229063 MS.gene020684:CDS 35.0%
AATGGTGACACAACAGATTG+TGG - chr5.4:74228643-74228662 None:intergenic 40.0%
ACCAAGATTATCAATGGAGG+AGG + chr5.4:74228913-74228932 MS.gene020684:CDS 40.0%
ACCATTCAAGAGTTCTGTCT+TGG + chr5.4:74228657-74228676 MS.gene020684:CDS 40.0%
CCATTCAAGAGTTCTGTCTT+GGG + chr5.4:74228658-74228677 MS.gene020684:CDS 40.0%
CGAAGGTTATCGTTAAACAC+GGG - chr5.4:74228946-74228965 None:intergenic 40.0%
CTTCTCTCAAATGTGAGATC+TGG + chr5.4:74228989-74229008 MS.gene020684:CDS 40.0%
GAAACAACACCATTACTGCT+AGG - chr5.4:74229483-74229502 None:intergenic 40.0%
GAAGGTTATCGTTAAACACG+GGG - chr5.4:74228945-74228964 None:intergenic 40.0%
GACAATTGAACAAAACCTGC+TGG - chr5.4:74229150-74229169 None:intergenic 40.0%
GCTGAAATTGCCATAACATC+TGG - chr5.4:74229195-74229214 None:intergenic 40.0%
GTTGAGTTTCCTGAACTAGT+TGG - chr5.4:74229789-74229808 None:intergenic 40.0%
TCCAATCAGGAGCAAATTTC+AGG + chr5.4:74229549-74229568 MS.gene020684:CDS 40.0%
TGAATCTGTTCAACCTCCTA+AGG + chr5.4:74229446-74229465 MS.gene020684:CDS 40.0%
TGCTGGTGAATGGAATAGAT+CGG - chr5.4:74229133-74229152 None:intergenic 40.0%
! ACCTGAAATTTGCTCCTGAT+TGG - chr5.4:74229553-74229572 None:intergenic 40.0%
! CTTCCTATGGACTTTGAAAG+TGG + chr5.4:74229762-74229781 MS.gene020684:CDS 40.0%
! TCCTCCTCCATTGATAATCT+TGG - chr5.4:74228917-74228936 None:intergenic 40.0%
! TTCACACTCACAACAGGTTT+TGG - chr5.4:74229648-74229667 None:intergenic 40.0%
!!! GCTTTTTTCGGAGTCTTTCT+CGG - chr5.4:74229250-74229269 None:intergenic 40.0%
AAGAGTTCTGTCTTGGGACA+TGG + chr5.4:74228664-74228683 MS.gene020684:CDS 45.0%
ACAACACCATTACTGCTAGG+TGG - chr5.4:74229480-74229499 None:intergenic 45.0%
ATATCCTGCTGCACCATCTT+TGG - chr5.4:74229684-74229703 None:intergenic 45.0%
CCCAAGACAGAACTCTTGAA+TGG - chr5.4:74228661-74228680 None:intergenic 45.0%
CTCATTCAGCAACGTATTCG+AGG - chr5.4:74229619-74229638 None:intergenic 45.0%
GAAAGTGGACCAACTAGTTC+AGG + chr5.4:74229777-74229796 MS.gene020684:CDS 45.0%
GAACAAAACCTGCTGGTGAA+TGG - chr5.4:74229143-74229162 None:intergenic 45.0%
GATCTATTCCATTCACCAGC+AGG + chr5.4:74229132-74229151 MS.gene020684:CDS 45.0%
GGTCCACTTTCAAAGTCCAT+AGG - chr5.4:74229768-74229787 None:intergenic 45.0%
TATTGAGCCAGAGCCAAAGA+TGG + chr5.4:74229668-74229687 MS.gene020684:CDS 45.0%
TGAGATCTGGATGCTTAGCA+GGG + chr5.4:74229002-74229021 MS.gene020684:CDS 45.0%
TGGTCCTAACACTTAGACGA+AGG - chr5.4:74228963-74228982 None:intergenic 45.0%
!! TGGTGTTGTTTCCTCTTCAG+TGG + chr5.4:74229491-74229510 MS.gene020684:CDS 45.0%
AAAAAAGCGAGTCGCACGTG+AGG + chr5.4:74229262-74229281 MS.gene020684:CDS 50.0%
AAAAAGCGAGTCGCACGTGA+GGG + chr5.4:74229263-74229282 MS.gene020684:CDS 50.0%
CTAGGTGGAGATGCAACCTT+AGG - chr5.4:74229465-74229484 None:intergenic 50.0%
GATGGTGCAGCAGGATATAG+TGG + chr5.4:74229686-74229705 MS.gene020684:CDS 50.0%
GCATCTCCACCTAGCAGTAA+TGG + chr5.4:74229471-74229490 MS.gene020684:CDS 50.0%
GTGAGATCTGGATGCTTAGC+AGG + chr5.4:74229001-74229020 MS.gene020684:CDS 50.0%
TGGCGCTTCACCAGATGTTA+TGG + chr5.4:74229182-74229201 MS.gene020684:CDS 50.0%
! TGTTGCAGTGTCGAATCCAG+AGG + chr5.4:74228765-74228784 MS.gene020684:CDS 50.0%
!!! CACGTGCGACTCGCTTTTTT+CGG - chr5.4:74229262-74229281 None:intergenic 50.0%
AGAGCCAAAGATGGTGCAGC+AGG + chr5.4:74229677-74229696 MS.gene020684:CDS 55.0%
GGTGGAGATGCAACCTTAGG+AGG - chr5.4:74229462-74229481 None:intergenic 55.0%
TGAGCTTGCAGCCACTGAAG+AGG - chr5.4:74229505-74229524 None:intergenic 55.0%
! TGCTGCACCATCTTTGGCTC+TGG - chr5.4:74229678-74229697 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 74228631 74229881 74228631 ID=MS.gene020684
chr5.4 mRNA 74228631 74229881 74228631 ID=MS.gene020684.t1;Parent=MS.gene020684
chr5.4 exon 74228631 74229881 74228631 ID=MS.gene020684.t1.exon1;Parent=MS.gene020684.t1
chr5.4 CDS 74228631 74229881 74228631 ID=cds.MS.gene020684.t1;Parent=MS.gene020684.t1
Gene Sequence

>MS.gene020684

ATGGAATCTCCACAATCTGTTGTGTCACCATTCAAGAGTTCTGTCTTGGGACATGGTGAGAAGTATAATTCTGATGTTTTGACACAAAGTTCTAGTCCTTTGTCTAAAGACATTGAGGTCAATGGAAAAGAAGCTGTTGCAGTGTCGAATCCAGAGGAATTTATTGGAGTAGTTGATGTTTACATACATCAAGCTAGGGACATTCATAACATCTGCATATACCATAAGCAAGATGTTTATGCTAAGATTTCTTTGACTAGTGATCCTGAAAATTTTGTCAATACCAAGATTATCAATGGAGGAGGAAGAAACCCCGTGTTTAACGATAACCTTCGTCTAAGTGTTAGGACCATTGATTCTTCTCTCAAATGTGAGATCTGGATGCTTAGCAGGGTGAAAAATTATCTTGAAGACCAATTACTTGGTTTTGCTTTGGTACCTTTGTCTGAAGTATTGATGAAAGATGGAAAACTAGAGAAAGAGTTCTCTCTTTCTTCGACCGATCTATTCCATTCACCAGCAGGTTTTGTTCAATTGTCACTTGCTTACAATGGCGCTTCACCAGATGTTATGGCAATTTCAGCAATGCCTAATACTGAATTGGACAGAAACGGCACCGAGAAAGACTCCGAAAAAAGCGAGTCGCACGTGAGGGATTTAGATAAAATAGAGTTCCCAAATCCAAAAATTGTAAATGAAGACCACTTAATGGTTTCAGAATACTTTGGTACTGATAGCTTGGCCACTACTGATACTGAAAATCTTAGTTCAGAAAAAGGTGTTCAACTTGTGGAAAGCTTCTCAGCATGTAGTGTTGAATCTGTTCAACCTCCTAAGGTTGCATCTCCACCTAGCAGTAATGGTGTTGTTTCCTCTTCAGTGGCTGCAAGCTCAGAATCACATGCTGCTACTAAATCTTCCAATCAGGAGCAAATTTCAGGTACAGAAGAGAAAAGAGTTGATCTCAAAGACGGCGAGAGTGATTCCTCGAATACGTTGCTGAATGAGTCATTTCCAAAACCTGTTGTGAGTGTGAATATTGAGCCAGAGCCAAAGATGGTGCAGCAGGATATAGTGGACATGTACTTGAAAAGTATGCAGCAATTTACCGAGTCATTAGCAAAAATGAAGCTTCCTATGGACTTTGAAAGTGGACCAACTAGTTCAGGAAACTCAACCTCTGAACAGAAACTACAATCTTCAAAGAGTAGTAACTCACGTGTGTTTTATGGTAGCAGAGCTTTCTTCTGA

Protein sequence

>MS.gene020684.t1

MESPQSVVSPFKSSVLGHGEKYNSDVLTQSSSPLSKDIEVNGKEAVAVSNPEEFIGVVDVYIHQARDIHNICIYHKQDVYAKISLTSDPENFVNTKIINGGGRNPVFNDNLRLSVRTIDSSLKCEIWMLSRVKNYLEDQLLGFALVPLSEVLMKDGKLEKEFSLSSTDLFHSPAGFVQLSLAYNGASPDVMAISAMPNTELDRNGTEKDSEKSESHVRDLDKIEFPNPKIVNEDHLMVSEYFGTDSLATTDTENLSSEKGVQLVESFSACSVESVQPPKVASPPSSNGVVSSSVAASSESHAATKSSNQEQISGTEEKRVDLKDGESDSSNTLLNESFPKPVVSVNIEPEPKMVQQDIVDMYLKSMQQFTESLAKMKLPMDFESGPTSSGNSTSEQKLQSSKSSNSRVFYGSRAFF