Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70895.t1 | XP_003617268.1 | 97.5 | 432 | 10 | 1 | 1 | 432 | 7 | 437 | 1.70E-234 | 821.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70895.t1 | G7K355 | 97.5 | 432 | 10 | 1 | 1 | 432 | 7 | 437 | 1.2e-234 | 821.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene70895 | 0.858369 | 8.64E-63 | -1.69E-46 |
| MS.gene049159 | MS.gene70895 | 0.849847 | 2.49E-60 | -1.69E-46 |
| MS.gene049254 | MS.gene70895 | 0.839916 | 1.19E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene70895 | 0.827755 | 1.31E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene70895 | 0.856742 | 2.62E-62 | -1.69E-46 |
| MS.gene049452 | MS.gene70895 | 0.804171 | 2.45E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene70895 | 0.832723 | 8.03E-56 | -1.69E-46 |
| MS.gene049585 | MS.gene70895 | 0.80103 | 1.09E-48 | -1.69E-46 |
| MS.gene049647 | MS.gene70895 | 0.840456 | 8.60E-58 | -1.69E-46 |
| MS.gene049711 | MS.gene70895 | 0.801496 | 8.76E-49 | -1.69E-46 |
| MS.gene049801 | MS.gene70895 | 0.804041 | 2.61E-49 | -1.69E-46 |
| MS.gene049958 | MS.gene70895 | 0.804664 | 1.94E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene70895 | 0.807834 | 4.16E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene70895 | 0.830907 | 2.25E-55 | -1.69E-46 |
| MS.gene050605 | MS.gene70895 | 0.802875 | 4.56E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene70895 | 0.809334 | 1.99E-50 | -1.69E-46 |
| MS.gene050759 | MS.gene70895 | 0.825639 | 4.20E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene70895 | 0.819266 | 1.27E-52 | -1.69E-46 |
| MS.gene050826 | MS.gene70895 | 0.803922 | 2.77E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene70895 | 0.8119 | 5.56E-51 | -1.69E-46 |
| MS.gene050962 | MS.gene70895 | 0.816938 | 4.27E-52 | -1.69E-46 |
| MS.gene051066 | MS.gene70895 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene051180 | MS.gene70895 | 0.813117 | 3.01E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene70895 | 0.866124 | 3.59E-65 | -1.69E-46 |
| MS.gene051210 | MS.gene70895 | 0.81652 | 5.30E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene70895 | 0.825519 | 4.48E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene70895 | 0.824126 | 9.56E-54 | -1.69E-46 |
| MS.gene05132 | MS.gene70895 | 0.818579 | 1.82E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene70895 | 0.800454 | 1.43E-48 | -1.69E-46 |
| MS.gene051388 | MS.gene70895 | 0.843434 | 1.40E-58 | -1.69E-46 |
| MS.gene051490 | MS.gene70895 | 0.816365 | 5.74E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene70895 | 0.805667 | 1.20E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene70895 | 0.802405 | 5.70E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene70895 | 0.833067 | 6.60E-56 | -1.69E-46 |
| MS.gene051800 | MS.gene70895 | 0.862683 | 4.26E-64 | -1.69E-46 |
| MS.gene052012 | MS.gene70895 | 0.818789 | 1.63E-52 | -1.69E-46 |
| MS.gene052020 | MS.gene70895 | 0.801447 | 8.97E-49 | -1.69E-46 |
| MS.gene052037 | MS.gene70895 | 0.811929 | 5.47E-51 | -1.69E-46 |
| MS.gene052042 | MS.gene70895 | 0.80608 | 9.79E-50 | -1.69E-46 |
| MS.gene052168 | MS.gene70895 | 0.808916 | 2.45E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene70895 | 0.803774 | 2.97E-49 | -1.69E-46 |
| MS.gene052311 | MS.gene70895 | 0.800471 | 1.42E-48 | -1.69E-46 |
| MS.gene052323 | MS.gene70895 | 0.813445 | 2.55E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene70895 | 0.821946 | 3.08E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene70895 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene70895 | 0.813307 | 2.73E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene70895 | 0.812769 | 3.59E-51 | -1.69E-46 |
| MS.gene052534 | MS.gene70895 | 0.807863 | 4.11E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene70895 | 0.81636 | 5.76E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene70895 | 0.817712 | 2.86E-52 | -1.69E-46 |
| MS.gene052586 | MS.gene70895 | 0.814575 | 1.44E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene70895 | 0.810452 | 1.14E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene70895 | 0.821796 | 3.34E-53 | -1.69E-46 |
| MS.gene052775 | MS.gene70895 | 0.803503 | 3.38E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene70895 | 0.833872 | 4.15E-56 | -1.69E-46 |
| MS.gene05288 | MS.gene70895 | 0.802604 | 5.19E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene70895 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene053079 | MS.gene70895 | 0.816311 | 5.90E-52 | -1.69E-46 |
| MS.gene05308 | MS.gene70895 | 0.813935 | 1.99E-51 | -1.69E-46 |
| MS.gene053147 | MS.gene70895 | 0.813413 | 2.59E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene70895 | 0.84034 | 9.22E-58 | -1.69E-46 |
| MS.gene053226 | MS.gene70895 | 0.838971 | 2.09E-57 | -1.69E-46 |
| MS.gene05350 | MS.gene70895 | 0.801146 | 1.03E-48 | -1.69E-46 |
| MS.gene053621 | MS.gene70895 | 0.838454 | 2.85E-57 | -1.69E-46 |
| MS.gene054139 | MS.gene70895 | 0.800449 | 1.43E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene70895 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene70895 | 0.836559 | 8.72E-57 | -1.69E-46 |
| MS.gene054291 | MS.gene70895 | -0.807753 | 4.33E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene70895 | 0.860812 | 1.59E-63 | -1.69E-46 |
| MS.gene054468 | MS.gene70895 | 0.828882 | 7.02E-55 | -1.69E-46 |
| MS.gene054472 | MS.gene70895 | 0.823483 | 1.35E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene70895 | 0.809505 | 1.83E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene70895 | 0.812934 | 3.30E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene70895 | 0.823216 | 1.56E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene70895 | 0.808038 | 3.77E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene70895 | 0.821743 | 3.43E-53 | -1.69E-46 |
| MS.gene054641 | MS.gene70895 | 0.80346 | 3.45E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene70895 | 0.849824 | 2.52E-60 | -1.69E-46 |
| MS.gene054658 | MS.gene70895 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene05468 | MS.gene70895 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene70895 | 0.867069 | 1.80E-65 | -1.69E-46 |
| MS.gene054801 | MS.gene70895 | 0.820672 | 6.06E-53 | -1.69E-46 |
| MS.gene054875 | MS.gene70895 | 0.801618 | 8.27E-49 | -1.69E-46 |
| MS.gene054991 | MS.gene70895 | 0.811963 | 5.38E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene70895 | 0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene055227 | MS.gene70895 | 0.829815 | 4.17E-55 | -1.69E-46 |
| MS.gene055228 | MS.gene70895 | 0.842298 | 2.82E-58 | -1.69E-46 |
| MS.gene055310 | MS.gene70895 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene70895 | 0.816302 | 5.93E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene70895 | 0.818388 | 2.01E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene70895 | 0.826708 | 2.34E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene70895 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene70895 | 0.818143 | 2.29E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene70895 | 0.826349 | 2.85E-54 | -1.69E-46 |
| MS.gene055623 | MS.gene70895 | 0.806976 | 6.33E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene70895 | 0.821264 | 4.43E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene70895 | 0.838745 | 2.40E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene70895 | 0.860452 | 2.04E-63 | -1.69E-46 |
| MS.gene05604 | MS.gene70895 | 0.845927 | 2.99E-59 | -1.69E-46 |
| MS.gene056050 | MS.gene70895 | 0.853579 | 2.18E-61 | -1.69E-46 |
| MS.gene056162 | MS.gene70895 | 0.817917 | 2.57E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene70895 | 0.843896 | 1.06E-58 | -1.69E-46 |
| MS.gene056174 | MS.gene70895 | 0.823089 | 1.67E-53 | -1.69E-46 |
| MS.gene056252 | MS.gene70895 | 0.813768 | 2.17E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene70895 | 0.817507 | 3.18E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene70895 | 0.82893 | 6.84E-55 | -1.69E-46 |
| MS.gene056373 | MS.gene70895 | 0.815266 | 1.01E-51 | -1.69E-46 |
| MS.gene056507 | MS.gene70895 | 0.815369 | 9.58E-52 | -1.69E-46 |
| MS.gene056553 | MS.gene70895 | 0.809841 | 1.55E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene70895 | 0.831428 | 1.68E-55 | -1.69E-46 |
| MS.gene05686 | MS.gene70895 | 0.8085 | 3.00E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene70895 | 0.80716 | 5.79E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene70895 | 0.810902 | 9.15E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene70895 | 0.812112 | 5.00E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene70895 | 0.810224 | 1.28E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene70895 | 0.826496 | 2.63E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene70895 | 0.832029 | 1.19E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene70895 | 0.849543 | 3.02E-60 | -1.69E-46 |
| MS.gene058146 | MS.gene70895 | 0.820454 | 6.80E-53 | -1.69E-46 |
| MS.gene058339 | MS.gene70895 | 0.816676 | 4.89E-52 | -1.69E-46 |
| MS.gene058360 | MS.gene70895 | 0.800077 | 1.71E-48 | -1.69E-46 |
| MS.gene05847 | MS.gene70895 | 0.83759 | 4.75E-57 | -1.69E-46 |
| MS.gene058515 | MS.gene70895 | 0.804843 | 1.78E-49 | -1.69E-46 |
| MS.gene058595 | MS.gene70895 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene70895 | 0.842465 | 2.54E-58 | -1.69E-46 |
| MS.gene058636 | MS.gene70895 | 0.800114 | 1.68E-48 | -1.69E-46 |
| MS.gene058781 | MS.gene70895 | 0.802931 | 4.44E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene70895 | 0.812351 | 4.43E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene70895 | 0.830688 | 2.55E-55 | -1.69E-46 |
| MS.gene05897 | MS.gene70895 | 0.808981 | 2.37E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene70895 | 0.813221 | 2.86E-51 | -1.69E-46 |
| MS.gene059438 | MS.gene70895 | 0.807293 | 5.43E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene70895 | 0.842287 | 2.84E-58 | -1.69E-46 |
| MS.gene059570 | MS.gene70895 | 0.847225 | 1.32E-59 | -1.69E-46 |
| MS.gene059584 | MS.gene70895 | 0.806547 | 7.80E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene70895 | 0.818928 | 1.52E-52 | -1.69E-46 |
| MS.gene059725 | MS.gene70895 | 0.830975 | 2.17E-55 | -1.69E-46 |
| MS.gene059842 | MS.gene70895 | 0.854358 | 1.30E-61 | -1.69E-46 |
| MS.gene060011 | MS.gene70895 | 0.840068 | 1.09E-57 | -1.69E-46 |
| MS.gene060137 | MS.gene70895 | -0.800488 | 1.41E-48 | -1.69E-46 |
| MS.gene060167 | MS.gene70895 | 0.808682 | 2.75E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene70895 | 0.838892 | 2.20E-57 | -1.69E-46 |
| MS.gene060312 | MS.gene70895 | 0.806674 | 7.34E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene70895 | 0.839818 | 1.26E-57 | -1.69E-46 |
| MS.gene060373 | MS.gene70895 | 0.808263 | 3.37E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene70895 | 0.866458 | 2.81E-65 | -1.69E-46 |
| MS.gene060637 | MS.gene70895 | 0.849325 | 3.48E-60 | -1.69E-46 |
| MS.gene060683 | MS.gene70895 | 0.801046 | 1.08E-48 | -1.69E-46 |
| MS.gene060685 | MS.gene70895 | 0.809568 | 1.77E-50 | -1.69E-46 |
| MS.gene060781 | MS.gene70895 | 0.806814 | 6.85E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene70895 | 0.831984 | 1.22E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene70895 | 0.838466 | 2.83E-57 | -1.69E-46 |
| MS.gene060998 | MS.gene70895 | 0.830408 | 2.99E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene48068 | MS.gene70895 | PPI |
| MS.gene70895 | MS.gene064623 | PPI |
| MS.gene63856 | MS.gene70895 | PPI |
| MS.gene90635 | MS.gene70895 | PPI |
| MS.gene020684 | MS.gene70895 | PPI |
| MS.gene66160 | MS.gene70895 | PPI |
| MS.gene81406 | MS.gene70895 | PPI |
| MS.gene68462 | MS.gene70895 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70895.t1 | MTR_5g089720 | 97.454 | 432 | 10 | 1 | 1 | 432 | 7 | 437 | 0.0 | 876 |
| MS.gene70895.t1 | MTR_5g094340 | 51.795 | 195 | 81 | 4 | 26 | 212 | 53 | 242 | 5.12e-62 | 206 |
| MS.gene70895.t1 | MTR_3g055960 | 48.718 | 195 | 94 | 3 | 26 | 216 | 54 | 246 | 3.83e-61 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70895.t1 | AT5G12300 | 56.204 | 274 | 86 | 6 | 27 | 290 | 19 | 268 | 1.15e-87 | 271 |
| MS.gene70895.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 23 | 432 | 33 | 388 | 1.18e-83 | 262 |
| MS.gene70895.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 23 | 432 | 33 | 388 | 1.18e-83 | 262 |
| MS.gene70895.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 26 | 218 | 48 | 242 | 7.56e-62 | 206 |
| MS.gene70895.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 26 | 218 | 48 | 242 | 7.56e-62 | 206 |
| MS.gene70895.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 26 | 218 | 48 | 242 | 7.56e-62 | 206 |
Find 59 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAACAATTTGTGAAAT+TGG | 0.112542 | 5.1:+70961849 | None:intergenic |
| CCGCTATGATCTTGTCTTTC+GGG | 0.138670 | 5.1:+70961027 | None:intergenic |
| AACTTCTTGTTCTCATGATT+TGG | 0.220949 | 5.1:+70960994 | None:intergenic |
| TGTGTAGCTCTATTCAAATT+TGG | 0.233288 | 5.1:+70962176 | None:intergenic |
| ACCGCTATGATCTTGTCTTT+CGG | 0.271993 | 5.1:+70961026 | None:intergenic |
| CTGTTATGAAATGTGAAATT+TGG | 0.279391 | 5.1:-70961929 | MS.gene70895:CDS |
| AGAAATCGACAATAAGAAAA+AGG | 0.318863 | 5.1:-70960971 | MS.gene70895:CDS |
| AGCATAGAAGTAACAAAGTT+TGG | 0.319974 | 5.1:-70961198 | MS.gene70895:CDS |
| TTCCAACAATTTGTGAAATT+GGG | 0.331184 | 5.1:+70961850 | None:intergenic |
| TTCTTTGAAGTTGGTGTATC+GGG | 0.348339 | 5.1:+70961297 | None:intergenic |
| ATTATCTTTAGACACATCTT+TGG | 0.351395 | 5.1:-70961751 | MS.gene70895:CDS |
| TCAATTCTTGAATACTCAAT+TGG | 0.352551 | 5.1:+70961648 | None:intergenic |
| TTGCTGCCATTTCTTCATCT+TGG | 0.384771 | 5.1:-70961531 | MS.gene70895:CDS |
| TCAAGATTAATATTCCCTAA+TGG | 0.389292 | 5.1:+70961159 | None:intergenic |
| TCCTGACATAAGTATTGTTA+AGG | 0.391070 | 5.1:-70961622 | MS.gene70895:CDS |
| GGGACAAAGAGTAACAAAAT+TGG | 0.398931 | 5.1:+70961317 | None:intergenic |
| GGAATTGTGAAGGCTTTCAT+TGG | 0.401534 | 5.1:+70961431 | None:intergenic |
| ATTTCACAAATTGTTGGAAA+AGG | 0.401795 | 5.1:-70961846 | MS.gene70895:CDS |
| CTTGTAGAAAGAGTTTCATC+AGG | 0.408913 | 5.1:+70962023 | None:intergenic |
| AATGAAAGCCTTCACAATTC+CGG | 0.426166 | 5.1:-70961429 | MS.gene70895:CDS |
| GTCCCAATTTCACAAATTGT+TGG | 0.426474 | 5.1:-70961852 | MS.gene70895:CDS |
| TCACCTTCTTTCTTTGAAGT+TGG | 0.429226 | 5.1:+70961288 | None:intergenic |
| TTTCTTTGAAGTTGGTGTAT+CGG | 0.431713 | 5.1:+70961296 | None:intergenic |
| GAGTTCGCCAGATGAGAATC+AGG | 0.437738 | 5.1:-70961469 | MS.gene70895:CDS |
| GAATCCACCTGATTCTCATC+TGG | 0.447663 | 5.1:+70961462 | None:intergenic |
| TCCTTAACAATACTTATGTC+AGG | 0.450696 | 5.1:+70961621 | None:intergenic |
| GTCATCAAAACAACAGCTTT+AGG | 0.454918 | 5.1:-70962205 | MS.gene70895:CDS |
| AAGCCTTCACAATTCCGGTT+TGG | 0.455785 | 5.1:-70961424 | MS.gene70895:CDS |
| GCAACAGTTTACTGAGTCCT+TGG | 0.468052 | 5.1:-70961082 | MS.gene70895:CDS |
| GAAGCACCAAGATGAAGAAA+TGG | 0.478364 | 5.1:+70961525 | None:intergenic |
| AAAATTCTCAAACAAAGAAA+AGG | 0.487388 | 5.1:-70961238 | MS.gene70895:CDS |
| GAATTGTGAAGGCTTTCATT+GGG | 0.490123 | 5.1:+70961432 | None:intergenic |
| TGTTAAGGAGAATCAACAAA+TGG | 0.492303 | 5.1:-70961607 | MS.gene70895:CDS |
| TGCTATGCAGAAGCAAATAG+TGG | 0.492416 | 5.1:-70961124 | MS.gene70895:CDS |
| AAAGATATCTGAAGTTATGT+TGG | 0.495670 | 5.1:-70961673 | MS.gene70895:CDS |
| GATAGTAGAGCTTACCAAAC+CGG | 0.496758 | 5.1:+70961410 | None:intergenic |
| AAAAGGAATGCAAGAAGGAA+AGG | 0.505483 | 5.1:-70961221 | MS.gene70895:CDS |
| CAAAGAAAAGGAATGCAAGA+AGG | 0.507838 | 5.1:-70961226 | MS.gene70895:CDS |
| GAAGATGAAGCAGAGTTCAC+AGG | 0.514799 | 5.1:-70962143 | MS.gene70895:CDS |
| GGCGAAAATGAAGCTTCCTA+TGG | 0.518944 | 5.1:-70961061 | MS.gene70895:CDS |
| TCTTGAATTGTTGTTACTTG+AGG | 0.524311 | 5.1:+70961557 | None:intergenic |
| TCGGGTTTGTCAACGTCCAT+AGG | 0.526209 | 5.1:+70961045 | None:intergenic |
| TTTCATCAGGGTTGTAAGTG+AGG | 0.531627 | 5.1:+70962036 | None:intergenic |
| GTTAATTGAACAGTTCCAGC+TGG | 0.532682 | 5.1:+70961774 | None:intergenic |
| CTTTCTACAAGAATAATCAA+TGG | 0.534660 | 5.1:-70962011 | MS.gene70895:CDS |
| AAATAGTGGATATGTATATG+AGG | 0.538443 | 5.1:-70961110 | MS.gene70895:CDS |
| ACAAGAATAATCAATGGAGG+TGG | 0.563554 | 5.1:-70962005 | MS.gene70895:CDS |
| TTCATCAGGGTTGTAAGTGA+GGG | 0.567857 | 5.1:+70962037 | None:intergenic |
| ACACCAACTTCAAAGAAAGA+AGG | 0.576636 | 5.1:-70961291 | MS.gene70895:CDS |
| TTACCAAACCGGAATTGTGA+AGG | 0.582333 | 5.1:+70961421 | None:intergenic |
| CAACATTCAATGAAAACTTG+AGG | 0.590629 | 5.1:-70961974 | MS.gene70895:CDS |
| GAATATTAATCTTGAAGCTG+AGG | 0.593724 | 5.1:-70961151 | MS.gene70895:CDS |
| AAGATGAAGCAGAGTTCACA+GGG | 0.598725 | 5.1:-70962142 | MS.gene70895:CDS |
| TGTTATGAATGTTTCTAGCA+TGG | 0.599484 | 5.1:+70962099 | None:intergenic |
| TTGTAGAAAGAGTTTCATCA+GGG | 0.607403 | 5.1:+70962024 | None:intergenic |
| TAGTAGAGCAAGAATTCACA+TGG | 0.626136 | 5.1:-70961901 | MS.gene70895:CDS |
| TCTACAAGAATAATCAATGG+AGG | 0.653951 | 5.1:-70962008 | MS.gene70895:CDS |
| TTCGCCAGATGAGAATCAGG+TGG | 0.666779 | 5.1:-70961466 | MS.gene70895:CDS |
| CCCGAAAGACAAGATCATAG+CGG | 0.770714 | 5.1:-70961027 | MS.gene70895:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTCAAACAAAGAAA+AGG | - | chr5.1:70961898-70961917 | MS.gene70895:CDS | 20.0% |
| !!! | AAGTTTTTAATTCTCCATTA+GGG | - | chr5.1:70961963-70961982 | MS.gene70895:CDS | 20.0% |
| ! | AGAAATCGACAATAAGAAAA+AGG | - | chr5.1:70962165-70962184 | MS.gene70895:CDS | 25.0% |
| ! | ATTATCTTTAGACACATCTT+TGG | - | chr5.1:70961385-70961404 | MS.gene70895:CDS | 25.0% |
| ! | ATTTCACAAATTGTTGGAAA+AGG | - | chr5.1:70961290-70961309 | MS.gene70895:CDS | 25.0% |
| ! | CTGTTATGAAATGTGAAATT+TGG | - | chr5.1:70961207-70961226 | MS.gene70895:CDS | 25.0% |
| ! | CTTTCTACAAGAATAATCAA+TGG | - | chr5.1:70961125-70961144 | MS.gene70895:CDS | 25.0% |
| ! | TCAAGATTAATATTCCCTAA+TGG | + | chr5.1:70961980-70961999 | None:intergenic | 25.0% |
| ! | TCAATTCTTGAATACTCAAT+TGG | + | chr5.1:70961491-70961510 | None:intergenic | 25.0% |
| ! | TTCCAACAATTTGTGAAATT+GGG | + | chr5.1:70961289-70961308 | None:intergenic | 25.0% |
| ! | TTTCCAACAATTTGTGAAAT+TGG | + | chr5.1:70961290-70961309 | None:intergenic | 25.0% |
| ! | TTTGAGAAAAAGAATCATCT+AGG | - | chr5.1:70961785-70961804 | MS.gene70895:CDS | 25.0% |
| !! | AAAGATATCTGAAGTTATGT+TGG | - | chr5.1:70961463-70961482 | MS.gene70895:CDS | 25.0% |
| !!! | AAATAGTGGATATGTATATG+AGG | - | chr5.1:70962026-70962045 | MS.gene70895:CDS | 25.0% |
| !!! | CAAGTTTTTAATTCTCCATT+AGG | - | chr5.1:70961962-70961981 | MS.gene70895:CDS | 25.0% |
| AACTTCTTGTTCTCATGATT+TGG | + | chr5.1:70962145-70962164 | None:intergenic | 30.0% | |
| AGCATAGAAGTAACAAAGTT+TGG | - | chr5.1:70961938-70961957 | MS.gene70895:CDS | 30.0% | |
| ATCGACAATAAGAAAAAGGA+TGG | - | chr5.1:70962169-70962188 | MS.gene70895:CDS | 30.0% | |
| CAACATTCAATGAAAACTTG+AGG | - | chr5.1:70961162-70961181 | MS.gene70895:CDS | 30.0% | |
| GAAAAAGATCAGTTGAAGAA+AGG | + | chr5.1:70961337-70961356 | None:intergenic | 30.0% | |
| GAATATTAATCTTGAAGCTG+AGG | - | chr5.1:70961985-70962004 | MS.gene70895:CDS | 30.0% | |
| TCCTTAACAATACTTATGTC+AGG | + | chr5.1:70961518-70961537 | None:intergenic | 30.0% | |
| TCTACAAGAATAATCAATGG+AGG | - | chr5.1:70961128-70961147 | MS.gene70895:CDS | 30.0% | |
| TGTTAAGGAGAATCAACAAA+TGG | - | chr5.1:70961529-70961548 | MS.gene70895:CDS | 30.0% | |
| TTGTAGAAAGAGTTTCATCA+GGG | + | chr5.1:70961115-70961134 | None:intergenic | 30.0% | |
| ! | ACATGGAAGATCAACTTTTA+GGG | - | chr5.1:70961252-70961271 | MS.gene70895:CDS | 30.0% |
| ! | CTCAAGTTTTCATTGAATGT+TGG | + | chr5.1:70961164-70961183 | None:intergenic | 30.0% |
| ! | TCCTGACATAAGTATTGTTA+AGG | - | chr5.1:70961514-70961533 | MS.gene70895:CDS | 30.0% |
| ! | TCTTGAATTGTTGTTACTTG+AGG | + | chr5.1:70961582-70961601 | None:intergenic | 30.0% |
| ! | TGTGTAGCTCTATTCAAATT+TGG | + | chr5.1:70960963-70960982 | None:intergenic | 30.0% |
| !! | TGTTATGAATGTTTCTAGCA+TGG | + | chr5.1:70961040-70961059 | None:intergenic | 30.0% |
| !! | TTTCTTTGAAGTTGGTGTAT+CGG | + | chr5.1:70961843-70961862 | None:intergenic | 30.0% |
| AAAAGGAATGCAAGAAGGAA+AGG | - | chr5.1:70961915-70961934 | MS.gene70895:CDS | 35.0% | |
| AATGAAAGCCTTCACAATTC+CGG | - | chr5.1:70961707-70961726 | MS.gene70895:CDS | 35.0% | |
| ACAAGAATAATCAATGGAGG+TGG | - | chr5.1:70961131-70961150 | MS.gene70895:CDS | 35.0% | |
| ACACCAACTTCAAAGAAAGA+AGG | - | chr5.1:70961845-70961864 | MS.gene70895:CDS | 35.0% | |
| CAAAGAAAAGGAATGCAAGA+AGG | - | chr5.1:70961910-70961929 | MS.gene70895:CDS | 35.0% | |
| CTTGTAGAAAGAGTTTCATC+AGG | + | chr5.1:70961116-70961135 | None:intergenic | 35.0% | |
| GAATTGTGAAGGCTTTCATT+GGG | + | chr5.1:70961707-70961726 | None:intergenic | 35.0% | |
| GGGACAAAGAGTAACAAAAT+TGG | + | chr5.1:70961822-70961841 | None:intergenic | 35.0% | |
| GTCATCAAAACAACAGCTTT+AGG | - | chr5.1:70960931-70960950 | MS.gene70895:CDS | 35.0% | |
| GTCCCAATTTCACAAATTGT+TGG | - | chr5.1:70961284-70961303 | MS.gene70895:CDS | 35.0% | |
| TAGTAGAGCAAGAATTCACA+TGG | - | chr5.1:70961235-70961254 | MS.gene70895:CDS | 35.0% | |
| ! | CACATGGAAGATCAACTTTT+AGG | - | chr5.1:70961251-70961270 | MS.gene70895:CDS | 35.0% |
| !! | TCACCTTCTTTCTTTGAAGT+TGG | + | chr5.1:70961851-70961870 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTTGGTGTATC+GGG | + | chr5.1:70961842-70961861 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | - | chr5.1:70961262-70961281 | MS.gene70895:CDS | 35.0% |
| AAGATGAAGCAGAGTTCACA+GGG | - | chr5.1:70960994-70961013 | MS.gene70895:CDS | 40.0% | |
| ACCGCTATGATCTTGTCTTT+CGG | + | chr5.1:70962113-70962132 | None:intergenic | 40.0% | |
| GATAGTAGAGCTTACCAAAC+CGG | + | chr5.1:70961729-70961748 | None:intergenic | 40.0% | |
| GGAATTGTGAAGGCTTTCAT+TGG | + | chr5.1:70961708-70961727 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | + | chr5.1:70961365-70961384 | None:intergenic | 40.0% | |
| TGCTATGCAGAAGCAAATAG+TGG | - | chr5.1:70962012-70962031 | MS.gene70895:CDS | 40.0% | |
| TTACCAAACCGGAATTGTGA+AGG | + | chr5.1:70961718-70961737 | None:intergenic | 40.0% | |
| TTCATCAGGGTTGTAAGTGA+GGG | + | chr5.1:70961102-70961121 | None:intergenic | 40.0% | |
| TTTCATCAGGGTTGTAAGTG+AGG | + | chr5.1:70961103-70961122 | None:intergenic | 40.0% | |
| ! | CTTTTTCTCAAACGAGTCTG+CGG | + | chr5.1:70961777-70961796 | None:intergenic | 40.0% |
| ! | GAAGCACCAAGATGAAGAAA+TGG | + | chr5.1:70961614-70961633 | None:intergenic | 40.0% |
| ! | TTGCTGCCATTTCTTCATCT+TGG | - | chr5.1:70961605-70961624 | MS.gene70895:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | - | chr5.1:70961347-70961366 | MS.gene70895:CDS | 40.0% |
| AAGCCTTCACAATTCCGGTT+TGG | - | chr5.1:70961712-70961731 | MS.gene70895:CDS | 45.0% | |
| CCCGAAAGACAAGATCATAG+CGG | - | chr5.1:70962109-70962128 | MS.gene70895:CDS | 45.0% | |
| CCGCTATGATCTTGTCTTTC+GGG | + | chr5.1:70962112-70962131 | None:intergenic | 45.0% | |
| GAAGATGAAGCAGAGTTCAC+AGG | - | chr5.1:70960993-70961012 | MS.gene70895:CDS | 45.0% | |
| GAATCCACCTGATTCTCATC+TGG | + | chr5.1:70961677-70961696 | None:intergenic | 45.0% | |
| GCAACAGTTTACTGAGTCCT+TGG | - | chr5.1:70962054-70962073 | MS.gene70895:CDS | 45.0% | |
| GGCGAAAATGAAGCTTCCTA+TGG | - | chr5.1:70962075-70962094 | MS.gene70895:CDS | 45.0% | |
| ! | AGGAAGCTTCATTTTCGCCA+AGG | + | chr5.1:70962074-70962093 | None:intergenic | 45.0% |
| ! | GATGGTTCGCGTGTGTTTTA+TGG | - | chr5.1:70962187-70962206 | MS.gene70895:CDS | 45.0% |
| GAGTTCGCCAGATGAGAATC+AGG | - | chr5.1:70961667-70961686 | MS.gene70895:CDS | 50.0% | |
| TCGGGTTTGTCAACGTCCAT+AGG | + | chr5.1:70962094-70962113 | None:intergenic | 50.0% | |
| TTCGCCAGATGAGAATCAGG+TGG | - | chr5.1:70961670-70961689 | MS.gene70895:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 70960930 | 70962228 | 70960930 | ID=MS.gene70895 |
| chr5.1 | mRNA | 70960930 | 70962228 | 70960930 | ID=MS.gene70895.t1;Parent=MS.gene70895 |
| chr5.1 | exon | 70960930 | 70962228 | 70960930 | ID=MS.gene70895.t1.exon1;Parent=MS.gene70895.t1 |
| chr5.1 | CDS | 70960930 | 70962228 | 70960930 | ID=cds.MS.gene70895.t1;Parent=MS.gene70895.t1 |
| Gene Sequence |
| Protein sequence |
