Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038202.t1 | XP_003617268.1 | 96.8 | 437 | 11 | 1 | 1 | 434 | 1 | 437 | 3.40E-235 | 823.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038202.t1 | G7K355 | 96.8 | 437 | 11 | 1 | 1 | 434 | 1 | 437 | 2.5e-235 | 823.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038202 | MS.gene48068 | PPI |
| MS.gene038202 | MS.gene63856 | PPI |
| MS.gene038202 | MS.gene90635 | PPI |
| MS.gene038202 | MS.gene66160 | PPI |
| MS.gene038202 | MS.gene81406 | PPI |
| MS.gene038202 | MS.gene020684 | PPI |
| MS.gene038202 | MS.gene064623 | PPI |
| MS.gene038202 | MS.gene68462 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038202.t1 | MTR_5g089720 | 96.796 | 437 | 11 | 1 | 1 | 434 | 1 | 437 | 0.0 | 877 |
| MS.gene038202.t1 | MTR_3g055960 | 36.298 | 416 | 215 | 9 | 32 | 434 | 54 | 432 | 3.02e-76 | 244 |
| MS.gene038202.t1 | MTR_5g094340 | 51.795 | 195 | 81 | 4 | 32 | 218 | 53 | 242 | 1.96e-62 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038202.t1 | AT5G12300 | 49.176 | 425 | 124 | 11 | 33 | 434 | 19 | 374 | 1.28e-108 | 325 |
| MS.gene038202.t1 | AT1G50570 | 39.803 | 407 | 193 | 9 | 29 | 434 | 33 | 388 | 2.20e-84 | 264 |
| MS.gene038202.t1 | AT1G50570 | 39.803 | 407 | 193 | 9 | 29 | 434 | 33 | 388 | 2.20e-84 | 264 |
| MS.gene038202.t1 | AT5G55530 | 50.761 | 197 | 91 | 3 | 32 | 224 | 48 | 242 | 8.52e-63 | 208 |
| MS.gene038202.t1 | AT5G55530 | 50.761 | 197 | 91 | 3 | 32 | 224 | 48 | 242 | 8.52e-63 | 208 |
| MS.gene038202.t1 | AT5G55530 | 50.761 | 197 | 91 | 3 | 32 | 224 | 48 | 242 | 8.52e-63 | 208 |
Find 57 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAACAATTTGTGAAAT+TGG | 0.112542 | 5.4:+70799154 | None:intergenic |
| CCGCTATGATCTTGTCTTTC+GGG | 0.138670 | 5.4:+70798344 | None:intergenic |
| AACTTCTTGTTCTCATGATT+TGG | 0.220949 | 5.4:+70798311 | None:intergenic |
| TGTGTAGCTCTATTCAAATT+TGG | 0.233288 | 5.4:+70799481 | None:intergenic |
| ACCGCTATGATCTTGTCTTT+CGG | 0.271993 | 5.4:+70798343 | None:intergenic |
| CTGTTATGAAATGTGAAATT+TGG | 0.279391 | 5.4:-70799234 | MS.gene038202:CDS |
| AGAAATCGACAATAAGAAAA+AGG | 0.318863 | 5.4:-70798288 | MS.gene038202:CDS |
| AGCATAGAAGTAACAAAGTT+TGG | 0.319974 | 5.4:-70798515 | MS.gene038202:CDS |
| TTCCAACAATTTGTGAAATT+GGG | 0.331184 | 5.4:+70799155 | None:intergenic |
| TTCTTTGAAGTTGGTGTATC+GGG | 0.348339 | 5.4:+70798602 | None:intergenic |
| AATGAAAGCCTTCACAATTC+CGG | 0.350419 | 5.4:-70798734 | MS.gene038202:CDS |
| ATTATCTTTAGACACATCTT+TGG | 0.351395 | 5.4:-70799056 | MS.gene038202:CDS |
| TCAATTCTTGAATACTCAAT+TGG | 0.352551 | 5.4:+70798953 | None:intergenic |
| TCCTGACATAAGTGTTGTTA+AGG | 0.358977 | 5.4:-70798927 | MS.gene038202:CDS |
| TCAAGATTAATATTTCCTAA+TGG | 0.368275 | 5.4:+70798476 | None:intergenic |
| TTGCTACCATTTCTTCATCT+TGG | 0.374030 | 5.4:-70798836 | MS.gene038202:CDS |
| ATTTCACAAATTGTTGGAAA+AGG | 0.401795 | 5.4:-70799151 | MS.gene038202:CDS |
| CTTGTAGAAAGAGTTTCATC+AGG | 0.408913 | 5.4:+70799328 | None:intergenic |
| TCACCTTCTTTCTTTGAAGT+TGG | 0.413213 | 5.4:+70798593 | None:intergenic |
| GTCCCAATTTCACAAATTGT+TGG | 0.426474 | 5.4:-70799157 | MS.gene038202:CDS |
| TTTCTTTGAAGTTGGTGTAT+CGG | 0.431713 | 5.4:+70798601 | None:intergenic |
| AAAAGGAATGCAAGAAAGAA+AGG | 0.435173 | 5.4:-70798538 | MS.gene038202:CDS |
| GAATCCACCTGATTCTCATC+TGG | 0.437208 | 5.4:+70798767 | None:intergenic |
| GAGTTCGCCAGATGAGAATC+AGG | 0.437738 | 5.4:-70798774 | MS.gene038202:CDS |
| GGGACAAAGAGTAACAAAAT+TGG | 0.453649 | 5.4:+70798622 | None:intergenic |
| GTCATCAAAACAACAGCTTT+AGG | 0.454918 | 5.4:-70799510 | MS.gene038202:CDS |
| AAGCCTTCACAATTCCGGTT+TGG | 0.455785 | 5.4:-70798729 | MS.gene038202:CDS |
| GCAACAGTTTACTGAGTCCT+TGG | 0.468052 | 5.4:-70798399 | MS.gene038202:CDS |
| AAAATTCTCAACCAAAGAAA+AGG | 0.474986 | 5.4:-70798555 | MS.gene038202:CDS |
| GAAGCACCAAGATGAAGAAA+TGG | 0.479734 | 5.4:+70798830 | None:intergenic |
| TCCTTAACAACACTTATGTC+AGG | 0.485333 | 5.4:+70798926 | None:intergenic |
| TGCTATGCAGAAGCAAATAG+TGG | 0.492416 | 5.4:-70798441 | MS.gene038202:CDS |
| AAAGATATCTGAAGTTATGT+TGG | 0.495670 | 5.4:-70798978 | MS.gene038202:CDS |
| GATAGTAGAGCTTACCAAAC+CGG | 0.496758 | 5.4:+70798715 | None:intergenic |
| GAATTGTGAAGGCTTTCATT+CGG | 0.498893 | 5.4:+70798737 | None:intergenic |
| TGTTAAGGAGAATCAACAAA+TGG | 0.505876 | 5.4:-70798912 | MS.gene038202:CDS |
| GAAGATGAAGCAGAGTTCAC+AGG | 0.514799 | 5.4:-70799448 | MS.gene038202:CDS |
| GGCGAAAATGAAGCTTCCTA+TGG | 0.518944 | 5.4:-70798378 | MS.gene038202:CDS |
| TCTTGAATTGTTGTTACTTG+AGG | 0.524311 | 5.4:+70798862 | None:intergenic |
| TCGGGTTTGTCAACGTCCAT+AGG | 0.526209 | 5.4:+70798362 | None:intergenic |
| TTTCATCAGGGTCGTAAGTG+AGG | 0.529931 | 5.4:+70799341 | None:intergenic |
| GTTAATTGAACAGTTCCAGC+TGG | 0.532682 | 5.4:+70799079 | None:intergenic |
| CTTTCTACAAGAATAATCAA+TGG | 0.534660 | 5.4:-70799316 | MS.gene038202:CDS |
| AAATAGTGGATATGTATATG+AGG | 0.538443 | 5.4:-70798427 | MS.gene038202:CDS |
| ACAAGAATAATCAATGGAGG+TGG | 0.563554 | 5.4:-70799310 | MS.gene038202:CDS |
| ACACCAACTTCAAAGAAAGA+AGG | 0.576636 | 5.4:-70798596 | MS.gene038202:CDS |
| AAATATTAATCTTGAAGCTG+AGG | 0.577321 | 5.4:-70798468 | MS.gene038202:CDS |
| TTACCAAACCGGAATTGTGA+AGG | 0.582333 | 5.4:+70798726 | None:intergenic |
| CAACATTCAATGAAAACTTG+AGG | 0.590629 | 5.4:-70799279 | MS.gene038202:CDS |
| TTCATCAGGGTCGTAAGTGA+GGG | 0.591372 | 5.4:+70799342 | None:intergenic |
| AAGATGAAGCAGAGTTCACA+GGG | 0.598725 | 5.4:-70799447 | MS.gene038202:CDS |
| TGTTATGAATGTTTCTAGCA+TGG | 0.599484 | 5.4:+70799404 | None:intergenic |
| TTGTAGAAAGAGTTTCATCA+GGG | 0.607403 | 5.4:+70799329 | None:intergenic |
| TAGTAGAGCAAGAATTCACA+TGG | 0.626136 | 5.4:-70799206 | MS.gene038202:CDS |
| TCTACAAGAATAATCAATGG+AGG | 0.653951 | 5.4:-70799313 | MS.gene038202:CDS |
| TTCGCCAGATGAGAATCAGG+TGG | 0.666779 | 5.4:-70798771 | MS.gene038202:CDS |
| CCCGAAAGACAAGATCATAG+CGG | 0.770714 | 5.4:-70798344 | MS.gene038202:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAGATTAATATTTCCTAA+TGG | + | chr5.4:70799303-70799322 | None:intergenic | 20.0% |
| !! | TTTGAGAAAAAGAATCATTT+AGG | - | chr5.4:70799120-70799139 | MS.gene038202:CDS | 20.0% |
| ! | AAAATTCTCAACCAAAGAAA+AGG | - | chr5.4:70799221-70799240 | MS.gene038202:CDS | 25.0% |
| ! | AAATATTAATCTTGAAGCTG+AGG | - | chr5.4:70799308-70799327 | MS.gene038202:CDS | 25.0% |
| ! | AGAAATCGACAATAAGAAAA+AGG | - | chr5.4:70799488-70799507 | MS.gene038202:CDS | 25.0% |
| ! | ATTATCTTTAGACACATCTT+TGG | - | chr5.4:70798720-70798739 | MS.gene038202:CDS | 25.0% |
| ! | ATTTCACAAATTGTTGGAAA+AGG | - | chr5.4:70798625-70798644 | MS.gene038202:CDS | 25.0% |
| ! | CTGTTATGAAATGTGAAATT+TGG | - | chr5.4:70798542-70798561 | MS.gene038202:CDS | 25.0% |
| ! | CTTTCTACAAGAATAATCAA+TGG | - | chr5.4:70798460-70798479 | MS.gene038202:CDS | 25.0% |
| ! | TCAATTCTTGAATACTCAAT+TGG | + | chr5.4:70798826-70798845 | None:intergenic | 25.0% |
| ! | TTCCAACAATTTGTGAAATT+GGG | + | chr5.4:70798624-70798643 | None:intergenic | 25.0% |
| ! | TTTCCAACAATTTGTGAAAT+TGG | + | chr5.4:70798625-70798644 | None:intergenic | 25.0% |
| !! | AAAGATATCTGAAGTTATGT+TGG | - | chr5.4:70798798-70798817 | MS.gene038202:CDS | 25.0% |
| !!! | AAATAGTGGATATGTATATG+AGG | - | chr5.4:70799349-70799368 | MS.gene038202:CDS | 25.0% |
| !!! | CAAGTTTTTAATTCTCCATT+AGG | - | chr5.4:70799285-70799304 | MS.gene038202:CDS | 25.0% |
| AAAAGGAATGCAAGAAAGAA+AGG | - | chr5.4:70799238-70799257 | MS.gene038202:CDS | 30.0% | |
| AACTTCTTGTTCTCATGATT+TGG | + | chr5.4:70799468-70799487 | None:intergenic | 30.0% | |
| AGCATAGAAGTAACAAAGTT+TGG | - | chr5.4:70799261-70799280 | MS.gene038202:CDS | 30.0% | |
| ATCGACAATAAGAAAAAGGA+TGG | - | chr5.4:70799492-70799511 | MS.gene038202:CDS | 30.0% | |
| CAACATTCAATGAAAACTTG+AGG | - | chr5.4:70798497-70798516 | MS.gene038202:CDS | 30.0% | |
| GAAAAAGATCAGTTGAAGAA+AGG | + | chr5.4:70798672-70798691 | None:intergenic | 30.0% | |
| TCTACAAGAATAATCAATGG+AGG | - | chr5.4:70798463-70798482 | MS.gene038202:CDS | 30.0% | |
| TGTTAAGGAGAATCAACAAA+TGG | - | chr5.4:70798864-70798883 | MS.gene038202:CDS | 30.0% | |
| TTGTAGAAAGAGTTTCATCA+GGG | + | chr5.4:70798450-70798469 | None:intergenic | 30.0% | |
| ! | ACATGGAAGATCAACTTTTA+GGG | - | chr5.4:70798587-70798606 | MS.gene038202:CDS | 30.0% |
| ! | CTCAAGTTTTCATTGAATGT+TGG | + | chr5.4:70798499-70798518 | None:intergenic | 30.0% |
| ! | TCTTGAATTGTTGTTACTTG+AGG | + | chr5.4:70798917-70798936 | None:intergenic | 30.0% |
| ! | TGTGTAGCTCTATTCAAATT+TGG | + | chr5.4:70798298-70798317 | None:intergenic | 30.0% |
| ! | TTTCTTGCATTCCTTTTCTT+TGG | + | chr5.4:70799235-70799254 | None:intergenic | 30.0% |
| !! | TGTTATGAATGTTTCTAGCA+TGG | + | chr5.4:70798375-70798394 | None:intergenic | 30.0% |
| !! | TTTCTTTGAAGTTGGTGTAT+CGG | + | chr5.4:70799178-70799197 | None:intergenic | 30.0% |
| AATGAAAGCCTTCACAATTC+CGG | - | chr5.4:70799042-70799061 | MS.gene038202:CDS | 35.0% | |
| ACAAGAATAATCAATGGAGG+TGG | - | chr5.4:70798466-70798485 | MS.gene038202:CDS | 35.0% | |
| ACACCAACTTCAAAGAAAGA+AGG | - | chr5.4:70799180-70799199 | MS.gene038202:CDS | 35.0% | |
| CTTGTAGAAAGAGTTTCATC+AGG | + | chr5.4:70798451-70798470 | None:intergenic | 35.0% | |
| GAATTGTGAAGGCTTTCATT+CGG | + | chr5.4:70799042-70799061 | None:intergenic | 35.0% | |
| GGGACAAAGAGTAACAAAAT+TGG | + | chr5.4:70799157-70799176 | None:intergenic | 35.0% | |
| GTCATCAAAACAACAGCTTT+AGG | - | chr5.4:70798266-70798285 | MS.gene038202:CDS | 35.0% | |
| GTCCCAATTTCACAAATTGT+TGG | - | chr5.4:70798619-70798638 | MS.gene038202:CDS | 35.0% | |
| TAGTAGAGCAAGAATTCACA+TGG | - | chr5.4:70798570-70798589 | MS.gene038202:CDS | 35.0% | |
| TCCTTAACAACACTTATGTC+AGG | + | chr5.4:70798853-70798872 | None:intergenic | 35.0% | |
| TTGCTACCATTTCTTCATCT+TGG | - | chr5.4:70798940-70798959 | MS.gene038202:CDS | 35.0% | |
| ! | CACATGGAAGATCAACTTTT+AGG | - | chr5.4:70798586-70798605 | MS.gene038202:CDS | 35.0% |
| ! | TCCTGACATAAGTGTTGTTA+AGG | - | chr5.4:70798849-70798868 | MS.gene038202:CDS | 35.0% |
| !! | TCACCTTCTTTCTTTGAAGT+TGG | + | chr5.4:70799186-70799205 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTTGGTGTATC+GGG | + | chr5.4:70799177-70799196 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | - | chr5.4:70798597-70798616 | MS.gene038202:CDS | 35.0% |
| AAGATGAAGCAGAGTTCACA+GGG | - | chr5.4:70798329-70798348 | MS.gene038202:CDS | 40.0% | |
| ACCGCTATGATCTTGTCTTT+CGG | + | chr5.4:70799436-70799455 | None:intergenic | 40.0% | |
| GATAGTAGAGCTTACCAAAC+CGG | + | chr5.4:70799064-70799083 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | + | chr5.4:70798700-70798719 | None:intergenic | 40.0% | |
| TGCTATGCAGAAGCAAATAG+TGG | - | chr5.4:70799335-70799354 | MS.gene038202:CDS | 40.0% | |
| TTACCAAACCGGAATTGTGA+AGG | + | chr5.4:70799053-70799072 | None:intergenic | 40.0% | |
| ! | CTTTTTCTCAAACGAGTCTG+CGG | + | chr5.4:70799112-70799131 | None:intergenic | 40.0% |
| ! | GAAGCACCAAGATGAAGAAA+TGG | + | chr5.4:70798949-70798968 | None:intergenic | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | - | chr5.4:70798682-70798701 | MS.gene038202:CDS | 40.0% |
| !! | GATGGTTTGCGTGTGTTTTA+TGG | - | chr5.4:70799510-70799529 | MS.gene038202:CDS | 40.0% |
| AAGCCTTCACAATTCCGGTT+TGG | - | chr5.4:70799047-70799066 | MS.gene038202:CDS | 45.0% | |
| CCCGAAAGACAAGATCATAG+CGG | - | chr5.4:70799432-70799451 | MS.gene038202:CDS | 45.0% | |
| CCGCTATGATCTTGTCTTTC+GGG | + | chr5.4:70799435-70799454 | None:intergenic | 45.0% | |
| GAAGATGAAGCAGAGTTCAC+AGG | - | chr5.4:70798328-70798347 | MS.gene038202:CDS | 45.0% | |
| GAATCCACCTGATTCTCATC+TGG | + | chr5.4:70799012-70799031 | None:intergenic | 45.0% | |
| GCAACAGTTTACTGAGTCCT+TGG | - | chr5.4:70799377-70799396 | MS.gene038202:CDS | 45.0% | |
| GGCGAAAATGAAGCTTCCTA+TGG | - | chr5.4:70799398-70799417 | MS.gene038202:CDS | 45.0% | |
| TTCATCAGGGTCGTAAGTGA+GGG | + | chr5.4:70798437-70798456 | None:intergenic | 45.0% | |
| TTTCATCAGGGTCGTAAGTG+AGG | + | chr5.4:70798438-70798457 | None:intergenic | 45.0% | |
| ! | AGGAAGCTTCATTTTCGCCA+AGG | + | chr5.4:70799397-70799416 | None:intergenic | 45.0% |
| GAGTTCGCCAGATGAGAATC+AGG | - | chr5.4:70799002-70799021 | MS.gene038202:CDS | 50.0% | |
| TCGGGTTTGTCAACGTCCAT+AGG | + | chr5.4:70799417-70799436 | None:intergenic | 50.0% | |
| TTCGCCAGATGAGAATCAGG+TGG | - | chr5.4:70799005-70799024 | MS.gene038202:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 70798247 | 70799551 | 70798247 | ID=MS.gene038202 |
| chr5.4 | mRNA | 70798247 | 70799551 | 70798247 | ID=MS.gene038202.t1;Parent=MS.gene038202 |
| chr5.4 | exon | 70798247 | 70799551 | 70798247 | ID=MS.gene038202.t1.exon1;Parent=MS.gene038202.t1 |
| chr5.4 | CDS | 70798247 | 70799551 | 70798247 | ID=cds.MS.gene038202.t1;Parent=MS.gene038202.t1 |
| Gene Sequence |
| Protein sequence |
