Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038143.t1 | XP_003617268.1 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 7.40E-238 | 832.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038143.t1 | G7K355 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 5.3e-238 | 832.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene48068 | MS.gene038143 | PPI |
| MS.gene038143 | MS.gene90635 | PPI |
| MS.gene66160 | MS.gene038143 | PPI |
| MS.gene038143 | MS.gene81406 | PPI |
| MS.gene68462 | MS.gene038143 | PPI |
| MS.gene038143 | MS.gene020684 | PPI |
| MS.gene038143 | MS.gene064623 | PPI |
| MS.gene63856 | MS.gene038143 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038143.t1 | MTR_5g089720 | 97.489 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 0.0 | 889 |
| MS.gene038143.t1 | MTR_5g094340 | 51.795 | 195 | 81 | 4 | 32 | 218 | 53 | 242 | 6.08e-62 | 206 |
| MS.gene038143.t1 | MTR_3g055960 | 48.718 | 195 | 94 | 3 | 32 | 222 | 54 | 246 | 3.95e-61 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038143.t1 | AT5G12300 | 56.204 | 274 | 86 | 6 | 33 | 296 | 19 | 268 | 1.52e-87 | 271 |
| MS.gene038143.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene038143.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene038143.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene038143.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene038143.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
Find 59 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAACAATTTGTGAAAT+TGG | 0.112542 | 5.1:+70927352 | None:intergenic |
| CCGCTATGATCTTGTCTTTC+GGG | 0.138670 | 5.1:+70926530 | None:intergenic |
| AACTTCTTGTTCTCATGATT+TGG | 0.220949 | 5.1:+70926497 | None:intergenic |
| TGTGTAGCTCTATTCAAATT+TGG | 0.233288 | 5.1:+70927679 | None:intergenic |
| ACCGCTATGATCTTGTCTTT+CGG | 0.271993 | 5.1:+70926529 | None:intergenic |
| CTGTTATGAAATGTGAAATT+TGG | 0.279391 | 5.1:-70927432 | MS.gene038143:CDS |
| AGAAATCGACAATAAGAAAA+AGG | 0.318863 | 5.1:-70926474 | MS.gene038143:CDS |
| AGCATAGAAGTAACAAAGTT+TGG | 0.319974 | 5.1:-70926701 | MS.gene038143:CDS |
| TTCCAACAATTTGTGAAATT+GGG | 0.331184 | 5.1:+70927353 | None:intergenic |
| TTCTTTGAAGTTGGTGTATC+GGG | 0.348339 | 5.1:+70926800 | None:intergenic |
| ATTATCTTTAGACACATCTT+TGG | 0.351395 | 5.1:-70927254 | MS.gene038143:CDS |
| TCAATTCTTGAATACTCAAT+TGG | 0.352551 | 5.1:+70927151 | None:intergenic |
| TTGCTGCCATTTCTTCATCT+TGG | 0.384771 | 5.1:-70927034 | MS.gene038143:CDS |
| TCAAGATTAATATTCCCTAA+TGG | 0.389292 | 5.1:+70926662 | None:intergenic |
| TCCTGACATAAGTATTGTTA+AGG | 0.391070 | 5.1:-70927125 | MS.gene038143:CDS |
| GGGACAAAGAGTAACAAAAT+TGG | 0.398931 | 5.1:+70926820 | None:intergenic |
| GGAATTGTGAAGGCTTTCAT+TGG | 0.401534 | 5.1:+70926934 | None:intergenic |
| ATTTCACAAATTGTTGGAAA+AGG | 0.401795 | 5.1:-70927349 | MS.gene038143:CDS |
| CTTGTAGAAAGAGTTTCATC+AGG | 0.408913 | 5.1:+70927526 | None:intergenic |
| AATGAAAGCCTTCACAATTC+CGG | 0.426166 | 5.1:-70926932 | MS.gene038143:CDS |
| GTCCCAATTTCACAAATTGT+TGG | 0.426474 | 5.1:-70927355 | MS.gene038143:CDS |
| TCACCTTCTTTCTTTGAAGT+TGG | 0.429226 | 5.1:+70926791 | None:intergenic |
| TTTCTTTGAAGTTGGTGTAT+CGG | 0.431713 | 5.1:+70926799 | None:intergenic |
| GAGTTCGCCAGATGAGAATC+AGG | 0.437738 | 5.1:-70926972 | MS.gene038143:CDS |
| GAATCCACCTGATTCTCATC+TGG | 0.447663 | 5.1:+70926965 | None:intergenic |
| TCCTTAACAATACTTATGTC+AGG | 0.450696 | 5.1:+70927124 | None:intergenic |
| GTCATCAAAACAACAGCTTT+AGG | 0.454918 | 5.1:-70927708 | MS.gene038143:CDS |
| AAGCCTTCACAATTCCGGTT+TGG | 0.455785 | 5.1:-70926927 | MS.gene038143:CDS |
| GCAACAGTTTACTGAGTCCT+TGG | 0.468052 | 5.1:-70926585 | MS.gene038143:CDS |
| GAAGCACCAAGATGAAGAAA+TGG | 0.478364 | 5.1:+70927028 | None:intergenic |
| AAAATTCTCAAACAAAGAAA+AGG | 0.487388 | 5.1:-70926741 | MS.gene038143:CDS |
| GAATTGTGAAGGCTTTCATT+GGG | 0.490123 | 5.1:+70926935 | None:intergenic |
| TGTTAAGGAGAATCAACAAA+TGG | 0.492303 | 5.1:-70927110 | MS.gene038143:CDS |
| TGCTATGCAGAAGCAAATAG+TGG | 0.492416 | 5.1:-70926627 | MS.gene038143:CDS |
| AAAGATATCTGAAGTTATGT+TGG | 0.495670 | 5.1:-70927176 | MS.gene038143:CDS |
| GATAGTAGAGCTTACCAAAC+CGG | 0.496758 | 5.1:+70926913 | None:intergenic |
| AAAAGGAATGCAAGAAGGAA+AGG | 0.505483 | 5.1:-70926724 | MS.gene038143:CDS |
| CAAAGAAAAGGAATGCAAGA+AGG | 0.507838 | 5.1:-70926729 | MS.gene038143:CDS |
| GAAGATGAAGCAGAGTTCAC+AGG | 0.514799 | 5.1:-70927646 | MS.gene038143:CDS |
| GGCGAAAATGAAGCTTCCTA+TGG | 0.518944 | 5.1:-70926564 | MS.gene038143:CDS |
| TCTTGAATTGTTGTTACTTG+AGG | 0.524311 | 5.1:+70927060 | None:intergenic |
| TCGGGTTTGTCAACGTCCAT+AGG | 0.526209 | 5.1:+70926548 | None:intergenic |
| TTTCATCAGGGTTGTAAGTG+AGG | 0.531627 | 5.1:+70927539 | None:intergenic |
| GTTAATTGAACAGTTCCAGC+TGG | 0.532682 | 5.1:+70927277 | None:intergenic |
| CTTTCTACAAGAATAATCAA+TGG | 0.534660 | 5.1:-70927514 | MS.gene038143:CDS |
| AAATAGTGGATATGTATATG+AGG | 0.538443 | 5.1:-70926613 | MS.gene038143:CDS |
| ACAAGAATAATCAATGGAGG+TGG | 0.563554 | 5.1:-70927508 | MS.gene038143:CDS |
| TTCATCAGGGTTGTAAGTGA+GGG | 0.567857 | 5.1:+70927540 | None:intergenic |
| ACACCAACTTCAAAGAAAGA+AGG | 0.576636 | 5.1:-70926794 | MS.gene038143:CDS |
| TTACCAAACCGGAATTGTGA+AGG | 0.582333 | 5.1:+70926924 | None:intergenic |
| CAACATTCAATGAAAACTTG+AGG | 0.590629 | 5.1:-70927477 | MS.gene038143:CDS |
| GAATATTAATCTTGAAGCTG+AGG | 0.593724 | 5.1:-70926654 | MS.gene038143:CDS |
| AAGATGAAGCAGAGTTCACA+GGG | 0.598725 | 5.1:-70927645 | MS.gene038143:CDS |
| TGTTATGAATGTTTCTAGCA+TGG | 0.599484 | 5.1:+70927602 | None:intergenic |
| TTGTAGAAAGAGTTTCATCA+GGG | 0.607403 | 5.1:+70927527 | None:intergenic |
| TAGTAGAGCAAGAATTCACA+TGG | 0.626136 | 5.1:-70927404 | MS.gene038143:CDS |
| TCTACAAGAATAATCAATGG+AGG | 0.653951 | 5.1:-70927511 | MS.gene038143:CDS |
| TTCGCCAGATGAGAATCAGG+TGG | 0.666779 | 5.1:-70926969 | MS.gene038143:CDS |
| CCCGAAAGACAAGATCATAG+CGG | 0.770714 | 5.1:-70926530 | MS.gene038143:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTCAAACAAAGAAA+AGG | - | chr5.1:70927419-70927438 | MS.gene038143:CDS | 20.0% |
| !!! | AAGTTTTTAATTCTCCATTA+GGG | - | chr5.1:70927484-70927503 | MS.gene038143:CDS | 20.0% |
| ! | AGAAATCGACAATAAGAAAA+AGG | - | chr5.1:70927686-70927705 | MS.gene038143:CDS | 25.0% |
| ! | ATTATCTTTAGACACATCTT+TGG | - | chr5.1:70926906-70926925 | MS.gene038143:CDS | 25.0% |
| ! | ATTTCACAAATTGTTGGAAA+AGG | - | chr5.1:70926811-70926830 | MS.gene038143:CDS | 25.0% |
| ! | CTGTTATGAAATGTGAAATT+TGG | - | chr5.1:70926728-70926747 | MS.gene038143:CDS | 25.0% |
| ! | CTTTCTACAAGAATAATCAA+TGG | - | chr5.1:70926646-70926665 | MS.gene038143:CDS | 25.0% |
| ! | TCAAGATTAATATTCCCTAA+TGG | + | chr5.1:70927501-70927520 | None:intergenic | 25.0% |
| ! | TCAATTCTTGAATACTCAAT+TGG | + | chr5.1:70927012-70927031 | None:intergenic | 25.0% |
| ! | TTCCAACAATTTGTGAAATT+GGG | + | chr5.1:70926810-70926829 | None:intergenic | 25.0% |
| ! | TTTCCAACAATTTGTGAAAT+TGG | + | chr5.1:70926811-70926830 | None:intergenic | 25.0% |
| ! | TTTGAGAAAAAGAATCATCT+AGG | - | chr5.1:70927306-70927325 | MS.gene038143:CDS | 25.0% |
| !! | AAAGATATCTGAAGTTATGT+TGG | - | chr5.1:70926984-70927003 | MS.gene038143:CDS | 25.0% |
| !!! | AAATAGTGGATATGTATATG+AGG | - | chr5.1:70927547-70927566 | MS.gene038143:CDS | 25.0% |
| !!! | CAAGTTTTTAATTCTCCATT+AGG | - | chr5.1:70927483-70927502 | MS.gene038143:CDS | 25.0% |
| AACTTCTTGTTCTCATGATT+TGG | + | chr5.1:70927666-70927685 | None:intergenic | 30.0% | |
| AGCATAGAAGTAACAAAGTT+TGG | - | chr5.1:70927459-70927478 | MS.gene038143:CDS | 30.0% | |
| ATCGACAATAAGAAAAAGGA+TGG | - | chr5.1:70927690-70927709 | MS.gene038143:CDS | 30.0% | |
| CAACATTCAATGAAAACTTG+AGG | - | chr5.1:70926683-70926702 | MS.gene038143:CDS | 30.0% | |
| GAAAAAGATCAGTTGAAGAA+AGG | + | chr5.1:70926858-70926877 | None:intergenic | 30.0% | |
| GAATATTAATCTTGAAGCTG+AGG | - | chr5.1:70927506-70927525 | MS.gene038143:CDS | 30.0% | |
| TCCTTAACAATACTTATGTC+AGG | + | chr5.1:70927039-70927058 | None:intergenic | 30.0% | |
| TCTACAAGAATAATCAATGG+AGG | - | chr5.1:70926649-70926668 | MS.gene038143:CDS | 30.0% | |
| TGTTAAGGAGAATCAACAAA+TGG | - | chr5.1:70927050-70927069 | MS.gene038143:CDS | 30.0% | |
| TTGTAGAAAGAGTTTCATCA+GGG | + | chr5.1:70926636-70926655 | None:intergenic | 30.0% | |
| ! | ACATGGAAGATCAACTTTTA+GGG | - | chr5.1:70926773-70926792 | MS.gene038143:CDS | 30.0% |
| ! | CTCAAGTTTTCATTGAATGT+TGG | + | chr5.1:70926685-70926704 | None:intergenic | 30.0% |
| ! | TCCTGACATAAGTATTGTTA+AGG | - | chr5.1:70927035-70927054 | MS.gene038143:CDS | 30.0% |
| ! | TCTTGAATTGTTGTTACTTG+AGG | + | chr5.1:70927103-70927122 | None:intergenic | 30.0% |
| ! | TGTGTAGCTCTATTCAAATT+TGG | + | chr5.1:70926484-70926503 | None:intergenic | 30.0% |
| !! | TGTTATGAATGTTTCTAGCA+TGG | + | chr5.1:70926561-70926580 | None:intergenic | 30.0% |
| !! | TTTCTTTGAAGTTGGTGTAT+CGG | + | chr5.1:70927364-70927383 | None:intergenic | 30.0% |
| AAAAGGAATGCAAGAAGGAA+AGG | - | chr5.1:70927436-70927455 | MS.gene038143:CDS | 35.0% | |
| AATGAAAGCCTTCACAATTC+CGG | - | chr5.1:70927228-70927247 | MS.gene038143:CDS | 35.0% | |
| ACAAGAATAATCAATGGAGG+TGG | - | chr5.1:70926652-70926671 | MS.gene038143:CDS | 35.0% | |
| ACACCAACTTCAAAGAAAGA+AGG | - | chr5.1:70927366-70927385 | MS.gene038143:CDS | 35.0% | |
| CAAAGAAAAGGAATGCAAGA+AGG | - | chr5.1:70927431-70927450 | MS.gene038143:CDS | 35.0% | |
| CTTGTAGAAAGAGTTTCATC+AGG | + | chr5.1:70926637-70926656 | None:intergenic | 35.0% | |
| GAATTGTGAAGGCTTTCATT+GGG | + | chr5.1:70927228-70927247 | None:intergenic | 35.0% | |
| GGGACAAAGAGTAACAAAAT+TGG | + | chr5.1:70927343-70927362 | None:intergenic | 35.0% | |
| GTCATCAAAACAACAGCTTT+AGG | - | chr5.1:70926452-70926471 | MS.gene038143:CDS | 35.0% | |
| GTCCCAATTTCACAAATTGT+TGG | - | chr5.1:70926805-70926824 | MS.gene038143:CDS | 35.0% | |
| TAGTAGAGCAAGAATTCACA+TGG | - | chr5.1:70926756-70926775 | MS.gene038143:CDS | 35.0% | |
| ! | CACATGGAAGATCAACTTTT+AGG | - | chr5.1:70926772-70926791 | MS.gene038143:CDS | 35.0% |
| !! | TCACCTTCTTTCTTTGAAGT+TGG | + | chr5.1:70927372-70927391 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTTGGTGTATC+GGG | + | chr5.1:70927363-70927382 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | - | chr5.1:70926783-70926802 | MS.gene038143:CDS | 35.0% |
| AAGATGAAGCAGAGTTCACA+GGG | - | chr5.1:70926515-70926534 | MS.gene038143:CDS | 40.0% | |
| ACCGCTATGATCTTGTCTTT+CGG | + | chr5.1:70927634-70927653 | None:intergenic | 40.0% | |
| GATAGTAGAGCTTACCAAAC+CGG | + | chr5.1:70927250-70927269 | None:intergenic | 40.0% | |
| GGAATTGTGAAGGCTTTCAT+TGG | + | chr5.1:70927229-70927248 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | + | chr5.1:70926886-70926905 | None:intergenic | 40.0% | |
| TGCTATGCAGAAGCAAATAG+TGG | - | chr5.1:70927533-70927552 | MS.gene038143:CDS | 40.0% | |
| TTACCAAACCGGAATTGTGA+AGG | + | chr5.1:70927239-70927258 | None:intergenic | 40.0% | |
| TTCATCAGGGTTGTAAGTGA+GGG | + | chr5.1:70926623-70926642 | None:intergenic | 40.0% | |
| TTTCATCAGGGTTGTAAGTG+AGG | + | chr5.1:70926624-70926643 | None:intergenic | 40.0% | |
| ! | CTTTTTCTCAAACGAGTCTG+CGG | + | chr5.1:70927298-70927317 | None:intergenic | 40.0% |
| ! | GAAGCACCAAGATGAAGAAA+TGG | + | chr5.1:70927135-70927154 | None:intergenic | 40.0% |
| ! | TTGCTGCCATTTCTTCATCT+TGG | - | chr5.1:70927126-70927145 | MS.gene038143:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | - | chr5.1:70926868-70926887 | MS.gene038143:CDS | 40.0% |
| AAGCCTTCACAATTCCGGTT+TGG | - | chr5.1:70927233-70927252 | MS.gene038143:CDS | 45.0% | |
| CCCGAAAGACAAGATCATAG+CGG | - | chr5.1:70927630-70927649 | MS.gene038143:CDS | 45.0% | |
| CCGCTATGATCTTGTCTTTC+GGG | + | chr5.1:70927633-70927652 | None:intergenic | 45.0% | |
| GAAGATGAAGCAGAGTTCAC+AGG | - | chr5.1:70926514-70926533 | MS.gene038143:CDS | 45.0% | |
| GAATCCACCTGATTCTCATC+TGG | + | chr5.1:70927198-70927217 | None:intergenic | 45.0% | |
| GCAACAGTTTACTGAGTCCT+TGG | - | chr5.1:70927575-70927594 | MS.gene038143:CDS | 45.0% | |
| GGCGAAAATGAAGCTTCCTA+TGG | - | chr5.1:70927596-70927615 | MS.gene038143:CDS | 45.0% | |
| ! | AGGAAGCTTCATTTTCGCCA+AGG | + | chr5.1:70927595-70927614 | None:intergenic | 45.0% |
| ! | GATGGTTCGCGTGTGTTTTA+TGG | - | chr5.1:70927708-70927727 | MS.gene038143:CDS | 45.0% |
| GAGTTCGCCAGATGAGAATC+AGG | - | chr5.1:70927188-70927207 | MS.gene038143:CDS | 50.0% | |
| TCGGGTTTGTCAACGTCCAT+AGG | + | chr5.1:70927615-70927634 | None:intergenic | 50.0% | |
| TTCGCCAGATGAGAATCAGG+TGG | - | chr5.1:70927191-70927210 | MS.gene038143:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 70926433 | 70927749 | 70926433 | ID=MS.gene038143 |
| chr5.1 | mRNA | 70926433 | 70927749 | 70926433 | ID=MS.gene038143.t1;Parent=MS.gene038143 |
| chr5.1 | exon | 70926433 | 70927749 | 70926433 | ID=MS.gene038143.t1.exon1;Parent=MS.gene038143.t1 |
| chr5.1 | CDS | 70926433 | 70927749 | 70926433 | ID=cds.MS.gene038143.t1;Parent=MS.gene038143.t1 |
| Gene Sequence |
| Protein sequence |
