Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47603.t1 | XP_003617268.1 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 7.40E-238 | 832.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47603.t1 | G7K355 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 5.3e-238 | 832.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene47603 | 0.856166 | 3.87E-62 | -1.69E-46 |
| MS.gene049159 | MS.gene47603 | 0.846047 | 2.78E-59 | -1.69E-46 |
| MS.gene049254 | MS.gene47603 | 0.842939 | 1.90E-58 | -1.69E-46 |
| MS.gene049404 | MS.gene47603 | 0.826868 | 2.14E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene47603 | 0.860545 | 1.92E-63 | -1.69E-46 |
| MS.gene049452 | MS.gene47603 | 0.807366 | 5.24E-50 | -1.69E-46 |
| MS.gene049501 | MS.gene47603 | 0.831248 | 1.86E-55 | -1.69E-46 |
| MS.gene049585 | MS.gene47603 | 0.800556 | 1.36E-48 | -1.69E-46 |
| MS.gene049647 | MS.gene47603 | 0.838675 | 2.50E-57 | -1.69E-46 |
| MS.gene049711 | MS.gene47603 | 0.801189 | 1.01E-48 | -1.69E-46 |
| MS.gene049744 | MS.gene47603 | 0.804949 | 1.69E-49 | -1.69E-46 |
| MS.gene049801 | MS.gene47603 | 0.807595 | 4.68E-50 | -1.69E-46 |
| MS.gene049958 | MS.gene47603 | 0.804143 | 2.49E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene47603 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene47603 | 0.832445 | 9.41E-56 | -1.69E-46 |
| MS.gene050605 | MS.gene47603 | 0.802327 | 5.91E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene47603 | 0.809157 | 2.17E-50 | -1.69E-46 |
| MS.gene050759 | MS.gene47603 | 0.827928 | 1.19E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene47603 | 0.818237 | 2.18E-52 | -1.69E-46 |
| MS.gene050826 | MS.gene47603 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene47603 | 0.802499 | 5.45E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene47603 | 0.820097 | 8.21E-53 | -1.69E-46 |
| MS.gene051066 | MS.gene47603 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene051180 | MS.gene47603 | 0.808652 | 2.79E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene47603 | 0.864765 | 9.61E-65 | -1.69E-46 |
| MS.gene051210 | MS.gene47603 | 0.806789 | 6.93E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene47603 | 0.82305 | 1.71E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene47603 | 0.828707 | 7.74E-55 | -1.69E-46 |
| MS.gene05132 | MS.gene47603 | 0.812309 | 4.52E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene47603 | 0.804131 | 2.50E-49 | -1.69E-46 |
| MS.gene051388 | MS.gene47603 | 0.837294 | 5.66E-57 | -1.69E-46 |
| MS.gene051490 | MS.gene47603 | 0.817074 | 3.98E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene47603 | 0.803552 | 3.30E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene47603 | 0.802117 | 6.53E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene47603 | 0.831343 | 1.76E-55 | -1.69E-46 |
| MS.gene051800 | MS.gene47603 | 0.861096 | 1.30E-63 | -1.69E-46 |
| MS.gene052012 | MS.gene47603 | 0.810152 | 1.33E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene47603 | 0.800665 | 1.30E-48 | -1.69E-46 |
| MS.gene052037 | MS.gene47603 | 0.808039 | 3.77E-50 | -1.69E-46 |
| MS.gene052168 | MS.gene47603 | 0.810325 | 1.22E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene47603 | 0.8036 | 3.23E-49 | -1.69E-46 |
| MS.gene052323 | MS.gene47603 | 0.805284 | 1.44E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene47603 | 0.818167 | 2.26E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene47603 | 0.811301 | 7.50E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene47603 | 0.813351 | 2.67E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene47603 | 0.809649 | 1.70E-50 | -1.69E-46 |
| MS.gene052534 | MS.gene47603 | 0.812562 | 3.98E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene47603 | 0.816728 | 4.76E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene47603 | 0.819442 | 1.16E-52 | -1.69E-46 |
| MS.gene052586 | MS.gene47603 | 0.808968 | 2.39E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene47603 | 0.800714 | 1.27E-48 | -1.69E-46 |
| MS.gene052773 | MS.gene47603 | 0.820211 | 7.73E-53 | -1.69E-46 |
| MS.gene052775 | MS.gene47603 | 0.80864 | 2.80E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene47603 | 0.832099 | 1.15E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene47603 | 0.814366 | 1.60E-51 | -1.69E-46 |
| MS.gene05308 | MS.gene47603 | 0.8152 | 1.04E-51 | -1.69E-46 |
| MS.gene053147 | MS.gene47603 | 0.813042 | 3.13E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene47603 | 0.838387 | 2.96E-57 | -1.69E-46 |
| MS.gene053226 | MS.gene47603 | 0.835292 | 1.83E-56 | -1.69E-46 |
| MS.gene05350 | MS.gene47603 | 0.803673 | 3.12E-49 | -1.69E-46 |
| MS.gene053621 | MS.gene47603 | 0.836734 | 7.87E-57 | -1.69E-46 |
| MS.gene05415 | MS.gene47603 | 0.80074 | 1.25E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene47603 | 0.833925 | 4.03E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene47603 | -0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene47603 | 0.858903 | 5.98E-63 | -1.69E-46 |
| MS.gene054468 | MS.gene47603 | 0.824259 | 8.89E-54 | -1.69E-46 |
| MS.gene054472 | MS.gene47603 | 0.819237 | 1.29E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene47603 | 0.807912 | 4.01E-50 | -1.69E-46 |
| MS.gene05447 | MS.gene47603 | 0.814696 | 1.35E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene47603 | 0.818854 | 1.58E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene47603 | 0.807697 | 4.45E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene47603 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene054641 | MS.gene47603 | 0.803412 | 3.53E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene47603 | 0.84998 | 2.28E-60 | -1.69E-46 |
| MS.gene05468 | MS.gene47603 | 0.80754 | 4.81E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene47603 | 0.867424 | 1.38E-65 | -1.69E-46 |
| MS.gene054801 | MS.gene47603 | 0.821686 | 3.54E-53 | -1.69E-46 |
| MS.gene054875 | MS.gene47603 | 0.800341 | 1.51E-48 | -1.69E-46 |
| MS.gene054991 | MS.gene47603 | 0.812336 | 4.46E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene47603 | 0.800913 | 1.15E-48 | -1.69E-46 |
| MS.gene055227 | MS.gene47603 | 0.823273 | 1.51E-53 | -1.69E-46 |
| MS.gene055228 | MS.gene47603 | 0.842749 | 2.14E-58 | -1.69E-46 |
| MS.gene055310 | MS.gene47603 | 0.803193 | 3.92E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene47603 | 0.812472 | 4.17E-51 | -1.69E-46 |
| MS.gene055357 | MS.gene47603 | 0.810338 | 1.21E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene47603 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene055466 | MS.gene47603 | 0.823548 | 1.31E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene47603 | 0.800821 | 1.20E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene47603 | 0.81922 | 1.30E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene47603 | 0.826097 | 3.27E-54 | -1.69E-46 |
| MS.gene055623 | MS.gene47603 | 0.802363 | 5.81E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene47603 | 0.822176 | 2.72E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene47603 | 0.831209 | 1.90E-55 | -1.69E-46 |
| MS.gene055926 | MS.gene47603 | 0.858751 | 6.64E-63 | -1.69E-46 |
| MS.gene05604 | MS.gene47603 | 0.842231 | 2.93E-58 | -1.69E-46 |
| MS.gene056050 | MS.gene47603 | 0.852117 | 5.70E-61 | -1.69E-46 |
| MS.gene056162 | MS.gene47603 | 0.81733 | 3.49E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene47603 | 0.841309 | 5.14E-58 | -1.69E-46 |
| MS.gene056174 | MS.gene47603 | 0.825631 | 4.22E-54 | -1.69E-46 |
| MS.gene056252 | MS.gene47603 | 0.812204 | 4.77E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene47603 | 0.817327 | 3.49E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene47603 | 0.820114 | 8.14E-53 | -1.69E-46 |
| MS.gene056373 | MS.gene47603 | 0.809396 | 1.93E-50 | -1.69E-46 |
| MS.gene056507 | MS.gene47603 | 0.817323 | 3.50E-52 | -1.69E-46 |
| MS.gene056553 | MS.gene47603 | 0.80392 | 2.77E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene47603 | 0.830857 | 2.32E-55 | -1.69E-46 |
| MS.gene05686 | MS.gene47603 | 0.812579 | 3.95E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene47603 | 0.802412 | 5.68E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene47603 | 0.81177 | 5.93E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene47603 | 0.812357 | 4.42E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene47603 | 0.810718 | 1.00E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene47603 | 0.803295 | 3.73E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene47603 | 0.828871 | 7.06E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene47603 | 0.830648 | 2.61E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene47603 | 0.852765 | 3.73E-61 | -1.69E-46 |
| MS.gene058146 | MS.gene47603 | 0.816781 | 4.63E-52 | -1.69E-46 |
| MS.gene058339 | MS.gene47603 | 0.81339 | 2.62E-51 | -1.69E-46 |
| MS.gene05847 | MS.gene47603 | 0.837429 | 5.22E-57 | -1.69E-46 |
| MS.gene058515 | MS.gene47603 | 0.804877 | 1.75E-49 | -1.69E-46 |
| MS.gene058595 | MS.gene47603 | 0.809048 | 2.29E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene47603 | 0.841643 | 4.20E-58 | -1.69E-46 |
| MS.gene058636 | MS.gene47603 | 0.801689 | 8.00E-49 | -1.69E-46 |
| MS.gene058647 | MS.gene47603 | 0.802728 | 4.89E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene47603 | 0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene47603 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene47603 | 0.828685 | 7.84E-55 | -1.69E-46 |
| MS.gene05897 | MS.gene47603 | 0.812882 | 3.39E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene47603 | 0.812833 | 3.48E-51 | -1.69E-46 |
| MS.gene059283 | MS.gene47603 | 0.800684 | 1.28E-48 | -1.69E-46 |
| MS.gene059438 | MS.gene47603 | 0.807647 | 4.56E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene47603 | 0.842636 | 2.29E-58 | -1.69E-46 |
| MS.gene059570 | MS.gene47603 | 0.843965 | 1.01E-58 | -1.69E-46 |
| MS.gene059584 | MS.gene47603 | 0.808248 | 3.40E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene47603 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene059725 | MS.gene47603 | 0.830972 | 2.17E-55 | -1.69E-46 |
| MS.gene059842 | MS.gene47603 | 0.853219 | 2.76E-61 | -1.69E-46 |
| MS.gene060011 | MS.gene47603 | 0.838429 | 2.89E-57 | -1.69E-46 |
| MS.gene060137 | MS.gene47603 | -0.803878 | 2.82E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene47603 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene47603 | 0.837971 | 3.79E-57 | -1.69E-46 |
| MS.gene060312 | MS.gene47603 | 0.804614 | 1.99E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene47603 | 0.837723 | 4.39E-57 | -1.69E-46 |
| MS.gene060373 | MS.gene47603 | 0.809028 | 2.32E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene47603 | 0.868396 | 6.73E-66 | -1.69E-46 |
| MS.gene060637 | MS.gene47603 | 0.842956 | 1.88E-58 | -1.69E-46 |
| MS.gene060683 | MS.gene47603 | 0.802913 | 4.48E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene47603 | 0.811586 | 6.50E-51 | -1.69E-46 |
| MS.gene060802 | MS.gene47603 | 0.832774 | 7.80E-56 | -1.69E-46 |
| MS.gene060803 | MS.gene47603 | 0.833698 | 4.59E-56 | -1.69E-46 |
| MS.gene060998 | MS.gene47603 | 0.834368 | 3.12E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47603 | MS.gene68462 | PPI |
| MS.gene47603 | MS.gene90635 | PPI |
| MS.gene47603 | MS.gene48068 | PPI |
| MS.gene47603 | MS.gene63856 | PPI |
| MS.gene47603 | MS.gene81406 | PPI |
| MS.gene47603 | MS.gene020684 | PPI |
| MS.gene66160 | MS.gene47603 | PPI |
| MS.gene47603 | MS.gene064623 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47603.t1 | MTR_5g089720 | 97.489 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 0.0 | 889 |
| MS.gene47603.t1 | MTR_5g094340 | 51.795 | 195 | 81 | 4 | 32 | 218 | 53 | 242 | 6.08e-62 | 206 |
| MS.gene47603.t1 | MTR_3g055960 | 48.718 | 195 | 94 | 3 | 32 | 222 | 54 | 246 | 3.95e-61 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47603.t1 | AT5G12300 | 56.204 | 274 | 86 | 6 | 33 | 296 | 19 | 268 | 1.52e-87 | 271 |
| MS.gene47603.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene47603.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene47603.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene47603.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene47603.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
Find 59 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAACAATTTGTGAAAT+TGG | 0.112542 | 5.1:+70946300 | None:intergenic |
| CCGCTATGATCTTGTCTTTC+GGG | 0.138670 | 5.1:+70945478 | None:intergenic |
| AACTTCTTGTTCTCATGATT+TGG | 0.220949 | 5.1:+70945445 | None:intergenic |
| TGTGTAGCTCTATTCAAATT+TGG | 0.233288 | 5.1:+70946627 | None:intergenic |
| ACCGCTATGATCTTGTCTTT+CGG | 0.271993 | 5.1:+70945477 | None:intergenic |
| CTGTTATGAAATGTGAAATT+TGG | 0.279391 | 5.1:-70946380 | MS.gene47603:CDS |
| AGAAATCGACAATAAGAAAA+AGG | 0.318863 | 5.1:-70945422 | MS.gene47603:CDS |
| AGCATAGAAGTAACAAAGTT+TGG | 0.319974 | 5.1:-70945649 | MS.gene47603:CDS |
| TTCCAACAATTTGTGAAATT+GGG | 0.331184 | 5.1:+70946301 | None:intergenic |
| TTCTTTGAAGTTGGTGTATC+GGG | 0.348339 | 5.1:+70945748 | None:intergenic |
| ATTATCTTTAGACACATCTT+TGG | 0.351395 | 5.1:-70946202 | MS.gene47603:CDS |
| TCAATTCTTGAATACTCAAT+TGG | 0.352551 | 5.1:+70946099 | None:intergenic |
| TTGCTGCCATTTCTTCATCT+TGG | 0.384771 | 5.1:-70945982 | MS.gene47603:CDS |
| TCAAGATTAATATTCCCTAA+TGG | 0.389292 | 5.1:+70945610 | None:intergenic |
| TCCTGACATAAGTATTGTTA+AGG | 0.391070 | 5.1:-70946073 | MS.gene47603:CDS |
| GGGACAAAGAGTAACAAAAT+TGG | 0.398931 | 5.1:+70945768 | None:intergenic |
| GGAATTGTGAAGGCTTTCAT+TGG | 0.401534 | 5.1:+70945882 | None:intergenic |
| ATTTCACAAATTGTTGGAAA+AGG | 0.401795 | 5.1:-70946297 | MS.gene47603:CDS |
| CTTGTAGAAAGAGTTTCATC+AGG | 0.408913 | 5.1:+70946474 | None:intergenic |
| AATGAAAGCCTTCACAATTC+CGG | 0.426166 | 5.1:-70945880 | MS.gene47603:CDS |
| GTCCCAATTTCACAAATTGT+TGG | 0.426474 | 5.1:-70946303 | MS.gene47603:CDS |
| TCACCTTCTTTCTTTGAAGT+TGG | 0.429226 | 5.1:+70945739 | None:intergenic |
| TTTCTTTGAAGTTGGTGTAT+CGG | 0.431713 | 5.1:+70945747 | None:intergenic |
| GAGTTCGCCAGATGAGAATC+AGG | 0.437738 | 5.1:-70945920 | MS.gene47603:CDS |
| GAATCCACCTGATTCTCATC+TGG | 0.447663 | 5.1:+70945913 | None:intergenic |
| TCCTTAACAATACTTATGTC+AGG | 0.450696 | 5.1:+70946072 | None:intergenic |
| GTCATCAAAACAACAGCTTT+AGG | 0.454918 | 5.1:-70946656 | MS.gene47603:CDS |
| AAGCCTTCACAATTCCGGTT+TGG | 0.455785 | 5.1:-70945875 | MS.gene47603:CDS |
| GCAACAGTTTACTGAGTCCT+TGG | 0.468052 | 5.1:-70945533 | MS.gene47603:CDS |
| GAAGCACCAAGATGAAGAAA+TGG | 0.478364 | 5.1:+70945976 | None:intergenic |
| AAAATTCTCAAACAAAGAAA+AGG | 0.487388 | 5.1:-70945689 | MS.gene47603:CDS |
| GAATTGTGAAGGCTTTCATT+GGG | 0.490123 | 5.1:+70945883 | None:intergenic |
| TGTTAAGGAGAATCAACAAA+TGG | 0.492303 | 5.1:-70946058 | MS.gene47603:CDS |
| TGCTATGCAGAAGCAAATAG+TGG | 0.492416 | 5.1:-70945575 | MS.gene47603:CDS |
| AAAGATATCTGAAGTTATGT+TGG | 0.495670 | 5.1:-70946124 | MS.gene47603:CDS |
| GATAGTAGAGCTTACCAAAC+CGG | 0.496758 | 5.1:+70945861 | None:intergenic |
| AAAAGGAATGCAAGAAGGAA+AGG | 0.505483 | 5.1:-70945672 | MS.gene47603:CDS |
| CAAAGAAAAGGAATGCAAGA+AGG | 0.507838 | 5.1:-70945677 | MS.gene47603:CDS |
| GAAGATGAAGCAGAGTTCAC+AGG | 0.514799 | 5.1:-70946594 | MS.gene47603:CDS |
| GGCGAAAATGAAGCTTCCTA+TGG | 0.518944 | 5.1:-70945512 | MS.gene47603:CDS |
| TCTTGAATTGTTGTTACTTG+AGG | 0.524311 | 5.1:+70946008 | None:intergenic |
| TCGGGTTTGTCAACGTCCAT+AGG | 0.526209 | 5.1:+70945496 | None:intergenic |
| TTTCATCAGGGTTGTAAGTG+AGG | 0.531627 | 5.1:+70946487 | None:intergenic |
| GTTAATTGAACAGTTCCAGC+TGG | 0.532682 | 5.1:+70946225 | None:intergenic |
| CTTTCTACAAGAATAATCAA+TGG | 0.534660 | 5.1:-70946462 | MS.gene47603:CDS |
| AAATAGTGGATATGTATATG+AGG | 0.538443 | 5.1:-70945561 | MS.gene47603:CDS |
| ACAAGAATAATCAATGGAGG+TGG | 0.563554 | 5.1:-70946456 | MS.gene47603:CDS |
| TTCATCAGGGTTGTAAGTGA+GGG | 0.567857 | 5.1:+70946488 | None:intergenic |
| ACACCAACTTCAAAGAAAGA+AGG | 0.576636 | 5.1:-70945742 | MS.gene47603:CDS |
| TTACCAAACCGGAATTGTGA+AGG | 0.582333 | 5.1:+70945872 | None:intergenic |
| CAACATTCAATGAAAACTTG+AGG | 0.590629 | 5.1:-70946425 | MS.gene47603:CDS |
| GAATATTAATCTTGAAGCTG+AGG | 0.593724 | 5.1:-70945602 | MS.gene47603:CDS |
| AAGATGAAGCAGAGTTCACA+GGG | 0.598725 | 5.1:-70946593 | MS.gene47603:CDS |
| TGTTATGAATGTTTCTAGCA+TGG | 0.599484 | 5.1:+70946550 | None:intergenic |
| TTGTAGAAAGAGTTTCATCA+GGG | 0.607403 | 5.1:+70946475 | None:intergenic |
| TAGTAGAGCAAGAATTCACA+TGG | 0.626136 | 5.1:-70946352 | MS.gene47603:CDS |
| TCTACAAGAATAATCAATGG+AGG | 0.653951 | 5.1:-70946459 | MS.gene47603:CDS |
| TTCGCCAGATGAGAATCAGG+TGG | 0.666779 | 5.1:-70945917 | MS.gene47603:CDS |
| CCCGAAAGACAAGATCATAG+CGG | 0.770714 | 5.1:-70945478 | MS.gene47603:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTCAAACAAAGAAA+AGG | - | chr5.1:70946367-70946386 | MS.gene47603:CDS | 20.0% |
| !!! | AAGTTTTTAATTCTCCATTA+GGG | - | chr5.1:70946432-70946451 | MS.gene47603:CDS | 20.0% |
| ! | AGAAATCGACAATAAGAAAA+AGG | - | chr5.1:70946634-70946653 | MS.gene47603:CDS | 25.0% |
| ! | ATTATCTTTAGACACATCTT+TGG | - | chr5.1:70945854-70945873 | MS.gene47603:CDS | 25.0% |
| ! | ATTTCACAAATTGTTGGAAA+AGG | - | chr5.1:70945759-70945778 | MS.gene47603:CDS | 25.0% |
| ! | CTGTTATGAAATGTGAAATT+TGG | - | chr5.1:70945676-70945695 | MS.gene47603:CDS | 25.0% |
| ! | CTTTCTACAAGAATAATCAA+TGG | - | chr5.1:70945594-70945613 | MS.gene47603:CDS | 25.0% |
| ! | TCAAGATTAATATTCCCTAA+TGG | + | chr5.1:70946449-70946468 | None:intergenic | 25.0% |
| ! | TCAATTCTTGAATACTCAAT+TGG | + | chr5.1:70945960-70945979 | None:intergenic | 25.0% |
| ! | TTCCAACAATTTGTGAAATT+GGG | + | chr5.1:70945758-70945777 | None:intergenic | 25.0% |
| ! | TTTCCAACAATTTGTGAAAT+TGG | + | chr5.1:70945759-70945778 | None:intergenic | 25.0% |
| ! | TTTGAGAAAAAGAATCATCT+AGG | - | chr5.1:70946254-70946273 | MS.gene47603:CDS | 25.0% |
| !! | AAAGATATCTGAAGTTATGT+TGG | - | chr5.1:70945932-70945951 | MS.gene47603:CDS | 25.0% |
| !!! | AAATAGTGGATATGTATATG+AGG | - | chr5.1:70946495-70946514 | MS.gene47603:CDS | 25.0% |
| !!! | CAAGTTTTTAATTCTCCATT+AGG | - | chr5.1:70946431-70946450 | MS.gene47603:CDS | 25.0% |
| AACTTCTTGTTCTCATGATT+TGG | + | chr5.1:70946614-70946633 | None:intergenic | 30.0% | |
| AGCATAGAAGTAACAAAGTT+TGG | - | chr5.1:70946407-70946426 | MS.gene47603:CDS | 30.0% | |
| ATCGACAATAAGAAAAAGGA+TGG | - | chr5.1:70946638-70946657 | MS.gene47603:CDS | 30.0% | |
| CAACATTCAATGAAAACTTG+AGG | - | chr5.1:70945631-70945650 | MS.gene47603:CDS | 30.0% | |
| GAAAAAGATCAGTTGAAGAA+AGG | + | chr5.1:70945806-70945825 | None:intergenic | 30.0% | |
| GAATATTAATCTTGAAGCTG+AGG | - | chr5.1:70946454-70946473 | MS.gene47603:CDS | 30.0% | |
| TCCTTAACAATACTTATGTC+AGG | + | chr5.1:70945987-70946006 | None:intergenic | 30.0% | |
| TCTACAAGAATAATCAATGG+AGG | - | chr5.1:70945597-70945616 | MS.gene47603:CDS | 30.0% | |
| TGTTAAGGAGAATCAACAAA+TGG | - | chr5.1:70945998-70946017 | MS.gene47603:CDS | 30.0% | |
| TTGTAGAAAGAGTTTCATCA+GGG | + | chr5.1:70945584-70945603 | None:intergenic | 30.0% | |
| ! | ACATGGAAGATCAACTTTTA+GGG | - | chr5.1:70945721-70945740 | MS.gene47603:CDS | 30.0% |
| ! | CTCAAGTTTTCATTGAATGT+TGG | + | chr5.1:70945633-70945652 | None:intergenic | 30.0% |
| ! | TCCTGACATAAGTATTGTTA+AGG | - | chr5.1:70945983-70946002 | MS.gene47603:CDS | 30.0% |
| ! | TCTTGAATTGTTGTTACTTG+AGG | + | chr5.1:70946051-70946070 | None:intergenic | 30.0% |
| ! | TGTGTAGCTCTATTCAAATT+TGG | + | chr5.1:70945432-70945451 | None:intergenic | 30.0% |
| !! | TGTTATGAATGTTTCTAGCA+TGG | + | chr5.1:70945509-70945528 | None:intergenic | 30.0% |
| !! | TTTCTTTGAAGTTGGTGTAT+CGG | + | chr5.1:70946312-70946331 | None:intergenic | 30.0% |
| AAAAGGAATGCAAGAAGGAA+AGG | - | chr5.1:70946384-70946403 | MS.gene47603:CDS | 35.0% | |
| AATGAAAGCCTTCACAATTC+CGG | - | chr5.1:70946176-70946195 | MS.gene47603:CDS | 35.0% | |
| ACAAGAATAATCAATGGAGG+TGG | - | chr5.1:70945600-70945619 | MS.gene47603:CDS | 35.0% | |
| ACACCAACTTCAAAGAAAGA+AGG | - | chr5.1:70946314-70946333 | MS.gene47603:CDS | 35.0% | |
| CAAAGAAAAGGAATGCAAGA+AGG | - | chr5.1:70946379-70946398 | MS.gene47603:CDS | 35.0% | |
| CTTGTAGAAAGAGTTTCATC+AGG | + | chr5.1:70945585-70945604 | None:intergenic | 35.0% | |
| GAATTGTGAAGGCTTTCATT+GGG | + | chr5.1:70946176-70946195 | None:intergenic | 35.0% | |
| GGGACAAAGAGTAACAAAAT+TGG | + | chr5.1:70946291-70946310 | None:intergenic | 35.0% | |
| GTCATCAAAACAACAGCTTT+AGG | - | chr5.1:70945400-70945419 | MS.gene47603:CDS | 35.0% | |
| GTCCCAATTTCACAAATTGT+TGG | - | chr5.1:70945753-70945772 | MS.gene47603:CDS | 35.0% | |
| TAGTAGAGCAAGAATTCACA+TGG | - | chr5.1:70945704-70945723 | MS.gene47603:CDS | 35.0% | |
| ! | CACATGGAAGATCAACTTTT+AGG | - | chr5.1:70945720-70945739 | MS.gene47603:CDS | 35.0% |
| !! | TCACCTTCTTTCTTTGAAGT+TGG | + | chr5.1:70946320-70946339 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTTGGTGTATC+GGG | + | chr5.1:70946311-70946330 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | - | chr5.1:70945731-70945750 | MS.gene47603:CDS | 35.0% |
| AAGATGAAGCAGAGTTCACA+GGG | - | chr5.1:70945463-70945482 | MS.gene47603:CDS | 40.0% | |
| ACCGCTATGATCTTGTCTTT+CGG | + | chr5.1:70946582-70946601 | None:intergenic | 40.0% | |
| GATAGTAGAGCTTACCAAAC+CGG | + | chr5.1:70946198-70946217 | None:intergenic | 40.0% | |
| GGAATTGTGAAGGCTTTCAT+TGG | + | chr5.1:70946177-70946196 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | + | chr5.1:70945834-70945853 | None:intergenic | 40.0% | |
| TGCTATGCAGAAGCAAATAG+TGG | - | chr5.1:70946481-70946500 | MS.gene47603:CDS | 40.0% | |
| TTACCAAACCGGAATTGTGA+AGG | + | chr5.1:70946187-70946206 | None:intergenic | 40.0% | |
| TTCATCAGGGTTGTAAGTGA+GGG | + | chr5.1:70945571-70945590 | None:intergenic | 40.0% | |
| TTTCATCAGGGTTGTAAGTG+AGG | + | chr5.1:70945572-70945591 | None:intergenic | 40.0% | |
| ! | CTTTTTCTCAAACGAGTCTG+CGG | + | chr5.1:70946246-70946265 | None:intergenic | 40.0% |
| ! | GAAGCACCAAGATGAAGAAA+TGG | + | chr5.1:70946083-70946102 | None:intergenic | 40.0% |
| ! | TTGCTGCCATTTCTTCATCT+TGG | - | chr5.1:70946074-70946093 | MS.gene47603:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | - | chr5.1:70945816-70945835 | MS.gene47603:CDS | 40.0% |
| AAGCCTTCACAATTCCGGTT+TGG | - | chr5.1:70946181-70946200 | MS.gene47603:CDS | 45.0% | |
| CCCGAAAGACAAGATCATAG+CGG | - | chr5.1:70946578-70946597 | MS.gene47603:CDS | 45.0% | |
| CCGCTATGATCTTGTCTTTC+GGG | + | chr5.1:70946581-70946600 | None:intergenic | 45.0% | |
| GAAGATGAAGCAGAGTTCAC+AGG | - | chr5.1:70945462-70945481 | MS.gene47603:CDS | 45.0% | |
| GAATCCACCTGATTCTCATC+TGG | + | chr5.1:70946146-70946165 | None:intergenic | 45.0% | |
| GCAACAGTTTACTGAGTCCT+TGG | - | chr5.1:70946523-70946542 | MS.gene47603:CDS | 45.0% | |
| GGCGAAAATGAAGCTTCCTA+TGG | - | chr5.1:70946544-70946563 | MS.gene47603:CDS | 45.0% | |
| ! | AGGAAGCTTCATTTTCGCCA+AGG | + | chr5.1:70946543-70946562 | None:intergenic | 45.0% |
| ! | GATGGTTCGCGTGTGTTTTA+TGG | - | chr5.1:70946656-70946675 | MS.gene47603:CDS | 45.0% |
| GAGTTCGCCAGATGAGAATC+AGG | - | chr5.1:70946136-70946155 | MS.gene47603:CDS | 50.0% | |
| TCGGGTTTGTCAACGTCCAT+AGG | + | chr5.1:70946563-70946582 | None:intergenic | 50.0% | |
| TTCGCCAGATGAGAATCAGG+TGG | - | chr5.1:70946139-70946158 | MS.gene47603:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 70945381 | 70946697 | 70945381 | ID=MS.gene47603 |
| chr5.1 | mRNA | 70945381 | 70946697 | 70945381 | ID=MS.gene47603.t1;Parent=MS.gene47603 |
| chr5.1 | exon | 70945381 | 70946697 | 70945381 | ID=MS.gene47603.t1.exon1;Parent=MS.gene47603.t1 |
| chr5.1 | CDS | 70945381 | 70946697 | 70945381 | ID=cds.MS.gene47603.t1;Parent=MS.gene47603.t1 |
| Gene Sequence |
| Protein sequence |
