Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038142.t1 | XP_003617268.1 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 7.40E-238 | 832.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038142.t1 | G7K355 | 97.5 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 5.3e-238 | 832.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90635 | MS.gene038142 | PPI |
| MS.gene020684 | MS.gene038142 | PPI |
| MS.gene48068 | MS.gene038142 | PPI |
| MS.gene66160 | MS.gene038142 | PPI |
| MS.gene038142 | MS.gene81406 | PPI |
| MS.gene68462 | MS.gene038142 | PPI |
| MS.gene038142 | MS.gene064623 | PPI |
| MS.gene63856 | MS.gene038142 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038142.t1 | MTR_5g089720 | 97.489 | 438 | 10 | 1 | 1 | 438 | 1 | 437 | 0.0 | 889 |
| MS.gene038142.t1 | MTR_5g094340 | 51.795 | 195 | 81 | 4 | 32 | 218 | 53 | 242 | 6.08e-62 | 206 |
| MS.gene038142.t1 | MTR_3g055960 | 48.718 | 195 | 94 | 3 | 32 | 222 | 54 | 246 | 3.95e-61 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038142.t1 | AT5G12300 | 56.204 | 274 | 86 | 6 | 33 | 296 | 19 | 268 | 1.52e-87 | 271 |
| MS.gene038142.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene038142.t1 | AT1G50570 | 40.389 | 411 | 189 | 11 | 29 | 438 | 33 | 388 | 1.02e-83 | 262 |
| MS.gene038142.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene038142.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
| MS.gene038142.t1 | AT5G55530 | 49.746 | 197 | 93 | 3 | 32 | 224 | 48 | 242 | 8.06e-62 | 206 |
Find 59 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAACAATTTGTGAAAT+TGG | 0.112542 | 5.1:+70870275 | None:intergenic |
| CCGCTATGATCTTGTCTTTC+GGG | 0.138670 | 5.1:+70869453 | None:intergenic |
| AACTTCTTGTTCTCATGATT+TGG | 0.220949 | 5.1:+70869420 | None:intergenic |
| TGTGTAGCTCTATTCAAATT+TGG | 0.233288 | 5.1:+70870602 | None:intergenic |
| ACCGCTATGATCTTGTCTTT+CGG | 0.271993 | 5.1:+70869452 | None:intergenic |
| CTGTTATGAAATGTGAAATT+TGG | 0.279391 | 5.1:-70870355 | MS.gene038142:CDS |
| AGAAATCGACAATAAGAAAA+AGG | 0.318863 | 5.1:-70869397 | MS.gene038142:CDS |
| AGCATAGAAGTAACAAAGTT+TGG | 0.319974 | 5.1:-70869624 | MS.gene038142:CDS |
| TTCCAACAATTTGTGAAATT+GGG | 0.331184 | 5.1:+70870276 | None:intergenic |
| TTCTTTGAAGTTGGTGTATC+GGG | 0.348339 | 5.1:+70869723 | None:intergenic |
| ATTATCTTTAGACACATCTT+TGG | 0.351395 | 5.1:-70870177 | MS.gene038142:CDS |
| TCAATTCTTGAATACTCAAT+TGG | 0.352551 | 5.1:+70870074 | None:intergenic |
| TTGCTGCCATTTCTTCATCT+TGG | 0.384771 | 5.1:-70869957 | MS.gene038142:CDS |
| TCAAGATTAATATTCCCTAA+TGG | 0.389292 | 5.1:+70869585 | None:intergenic |
| TCCTGACATAAGTATTGTTA+AGG | 0.391070 | 5.1:-70870048 | MS.gene038142:CDS |
| GGGACAAAGAGTAACAAAAT+TGG | 0.398931 | 5.1:+70869743 | None:intergenic |
| GGAATTGTGAAGGCTTTCAT+TGG | 0.401534 | 5.1:+70869857 | None:intergenic |
| ATTTCACAAATTGTTGGAAA+AGG | 0.401795 | 5.1:-70870272 | MS.gene038142:CDS |
| CTTGTAGAAAGAGTTTCATC+AGG | 0.408913 | 5.1:+70870449 | None:intergenic |
| AATGAAAGCCTTCACAATTC+CGG | 0.426166 | 5.1:-70869855 | MS.gene038142:CDS |
| GTCCCAATTTCACAAATTGT+TGG | 0.426474 | 5.1:-70870278 | MS.gene038142:CDS |
| TCACCTTCTTTCTTTGAAGT+TGG | 0.429226 | 5.1:+70869714 | None:intergenic |
| TTTCTTTGAAGTTGGTGTAT+CGG | 0.431713 | 5.1:+70869722 | None:intergenic |
| GAGTTCGCCAGATGAGAATC+AGG | 0.437738 | 5.1:-70869895 | MS.gene038142:CDS |
| GAATCCACCTGATTCTCATC+TGG | 0.447663 | 5.1:+70869888 | None:intergenic |
| TCCTTAACAATACTTATGTC+AGG | 0.450696 | 5.1:+70870047 | None:intergenic |
| GTCATCAAAACAACAGCTTT+AGG | 0.454918 | 5.1:-70870631 | MS.gene038142:CDS |
| AAGCCTTCACAATTCCGGTT+TGG | 0.455785 | 5.1:-70869850 | MS.gene038142:CDS |
| GCAACAGTTTACTGAGTCCT+TGG | 0.468052 | 5.1:-70869508 | MS.gene038142:CDS |
| GAAGCACCAAGATGAAGAAA+TGG | 0.478364 | 5.1:+70869951 | None:intergenic |
| AAAATTCTCAAACAAAGAAA+AGG | 0.487388 | 5.1:-70869664 | MS.gene038142:CDS |
| GAATTGTGAAGGCTTTCATT+GGG | 0.490123 | 5.1:+70869858 | None:intergenic |
| TGTTAAGGAGAATCAACAAA+TGG | 0.492303 | 5.1:-70870033 | MS.gene038142:CDS |
| TGCTATGCAGAAGCAAATAG+TGG | 0.492416 | 5.1:-70869550 | MS.gene038142:CDS |
| AAAGATATCTGAAGTTATGT+TGG | 0.495670 | 5.1:-70870099 | MS.gene038142:CDS |
| GATAGTAGAGCTTACCAAAC+CGG | 0.496758 | 5.1:+70869836 | None:intergenic |
| AAAAGGAATGCAAGAAGGAA+AGG | 0.505483 | 5.1:-70869647 | MS.gene038142:CDS |
| CAAAGAAAAGGAATGCAAGA+AGG | 0.507838 | 5.1:-70869652 | MS.gene038142:CDS |
| GAAGATGAAGCAGAGTTCAC+AGG | 0.514799 | 5.1:-70870569 | MS.gene038142:CDS |
| GGCGAAAATGAAGCTTCCTA+TGG | 0.518944 | 5.1:-70869487 | MS.gene038142:CDS |
| TCTTGAATTGTTGTTACTTG+AGG | 0.524311 | 5.1:+70869983 | None:intergenic |
| TCGGGTTTGTCAACGTCCAT+AGG | 0.526209 | 5.1:+70869471 | None:intergenic |
| TTTCATCAGGGTTGTAAGTG+AGG | 0.531627 | 5.1:+70870462 | None:intergenic |
| GTTAATTGAACAGTTCCAGC+TGG | 0.532682 | 5.1:+70870200 | None:intergenic |
| CTTTCTACAAGAATAATCAA+TGG | 0.534660 | 5.1:-70870437 | MS.gene038142:CDS |
| AAATAGTGGATATGTATATG+AGG | 0.538443 | 5.1:-70869536 | MS.gene038142:CDS |
| ACAAGAATAATCAATGGAGG+TGG | 0.563554 | 5.1:-70870431 | MS.gene038142:CDS |
| TTCATCAGGGTTGTAAGTGA+GGG | 0.567857 | 5.1:+70870463 | None:intergenic |
| ACACCAACTTCAAAGAAAGA+AGG | 0.576636 | 5.1:-70869717 | MS.gene038142:CDS |
| TTACCAAACCGGAATTGTGA+AGG | 0.582333 | 5.1:+70869847 | None:intergenic |
| CAACATTCAATGAAAACTTG+AGG | 0.590629 | 5.1:-70870400 | MS.gene038142:CDS |
| GAATATTAATCTTGAAGCTG+AGG | 0.593724 | 5.1:-70869577 | MS.gene038142:CDS |
| AAGATGAAGCAGAGTTCACA+GGG | 0.598725 | 5.1:-70870568 | MS.gene038142:CDS |
| TGTTATGAATGTTTCTAGCA+TGG | 0.599484 | 5.1:+70870525 | None:intergenic |
| TTGTAGAAAGAGTTTCATCA+GGG | 0.607403 | 5.1:+70870450 | None:intergenic |
| TAGTAGAGCAAGAATTCACA+TGG | 0.626136 | 5.1:-70870327 | MS.gene038142:CDS |
| TCTACAAGAATAATCAATGG+AGG | 0.653951 | 5.1:-70870434 | MS.gene038142:CDS |
| TTCGCCAGATGAGAATCAGG+TGG | 0.666779 | 5.1:-70869892 | MS.gene038142:CDS |
| CCCGAAAGACAAGATCATAG+CGG | 0.770714 | 5.1:-70869453 | MS.gene038142:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTCAAACAAAGAAA+AGG | - | chr5.1:70870342-70870361 | MS.gene038142:CDS | 20.0% |
| !!! | AAGTTTTTAATTCTCCATTA+GGG | - | chr5.1:70870407-70870426 | MS.gene038142:CDS | 20.0% |
| ! | AGAAATCGACAATAAGAAAA+AGG | - | chr5.1:70870609-70870628 | MS.gene038142:CDS | 25.0% |
| ! | ATTATCTTTAGACACATCTT+TGG | - | chr5.1:70869829-70869848 | MS.gene038142:CDS | 25.0% |
| ! | ATTTCACAAATTGTTGGAAA+AGG | - | chr5.1:70869734-70869753 | MS.gene038142:CDS | 25.0% |
| ! | CTGTTATGAAATGTGAAATT+TGG | - | chr5.1:70869651-70869670 | MS.gene038142:CDS | 25.0% |
| ! | CTTTCTACAAGAATAATCAA+TGG | - | chr5.1:70869569-70869588 | MS.gene038142:CDS | 25.0% |
| ! | TCAAGATTAATATTCCCTAA+TGG | + | chr5.1:70870424-70870443 | None:intergenic | 25.0% |
| ! | TCAATTCTTGAATACTCAAT+TGG | + | chr5.1:70869935-70869954 | None:intergenic | 25.0% |
| ! | TTCCAACAATTTGTGAAATT+GGG | + | chr5.1:70869733-70869752 | None:intergenic | 25.0% |
| ! | TTTCCAACAATTTGTGAAAT+TGG | + | chr5.1:70869734-70869753 | None:intergenic | 25.0% |
| ! | TTTGAGAAAAAGAATCATCT+AGG | - | chr5.1:70870229-70870248 | MS.gene038142:CDS | 25.0% |
| !! | AAAGATATCTGAAGTTATGT+TGG | - | chr5.1:70869907-70869926 | MS.gene038142:CDS | 25.0% |
| !!! | AAATAGTGGATATGTATATG+AGG | - | chr5.1:70870470-70870489 | MS.gene038142:CDS | 25.0% |
| !!! | CAAGTTTTTAATTCTCCATT+AGG | - | chr5.1:70870406-70870425 | MS.gene038142:CDS | 25.0% |
| AACTTCTTGTTCTCATGATT+TGG | + | chr5.1:70870589-70870608 | None:intergenic | 30.0% | |
| AGCATAGAAGTAACAAAGTT+TGG | - | chr5.1:70870382-70870401 | MS.gene038142:CDS | 30.0% | |
| ATCGACAATAAGAAAAAGGA+TGG | - | chr5.1:70870613-70870632 | MS.gene038142:CDS | 30.0% | |
| CAACATTCAATGAAAACTTG+AGG | - | chr5.1:70869606-70869625 | MS.gene038142:CDS | 30.0% | |
| GAAAAAGATCAGTTGAAGAA+AGG | + | chr5.1:70869781-70869800 | None:intergenic | 30.0% | |
| GAATATTAATCTTGAAGCTG+AGG | - | chr5.1:70870429-70870448 | MS.gene038142:CDS | 30.0% | |
| TCCTTAACAATACTTATGTC+AGG | + | chr5.1:70869962-70869981 | None:intergenic | 30.0% | |
| TCTACAAGAATAATCAATGG+AGG | - | chr5.1:70869572-70869591 | MS.gene038142:CDS | 30.0% | |
| TGTTAAGGAGAATCAACAAA+TGG | - | chr5.1:70869973-70869992 | MS.gene038142:CDS | 30.0% | |
| TTGTAGAAAGAGTTTCATCA+GGG | + | chr5.1:70869559-70869578 | None:intergenic | 30.0% | |
| ! | ACATGGAAGATCAACTTTTA+GGG | - | chr5.1:70869696-70869715 | MS.gene038142:CDS | 30.0% |
| ! | CTCAAGTTTTCATTGAATGT+TGG | + | chr5.1:70869608-70869627 | None:intergenic | 30.0% |
| ! | TCCTGACATAAGTATTGTTA+AGG | - | chr5.1:70869958-70869977 | MS.gene038142:CDS | 30.0% |
| ! | TCTTGAATTGTTGTTACTTG+AGG | + | chr5.1:70870026-70870045 | None:intergenic | 30.0% |
| ! | TGTGTAGCTCTATTCAAATT+TGG | + | chr5.1:70869407-70869426 | None:intergenic | 30.0% |
| !! | TGTTATGAATGTTTCTAGCA+TGG | + | chr5.1:70869484-70869503 | None:intergenic | 30.0% |
| !! | TTTCTTTGAAGTTGGTGTAT+CGG | + | chr5.1:70870287-70870306 | None:intergenic | 30.0% |
| AAAAGGAATGCAAGAAGGAA+AGG | - | chr5.1:70870359-70870378 | MS.gene038142:CDS | 35.0% | |
| AATGAAAGCCTTCACAATTC+CGG | - | chr5.1:70870151-70870170 | MS.gene038142:CDS | 35.0% | |
| ACAAGAATAATCAATGGAGG+TGG | - | chr5.1:70869575-70869594 | MS.gene038142:CDS | 35.0% | |
| ACACCAACTTCAAAGAAAGA+AGG | - | chr5.1:70870289-70870308 | MS.gene038142:CDS | 35.0% | |
| CAAAGAAAAGGAATGCAAGA+AGG | - | chr5.1:70870354-70870373 | MS.gene038142:CDS | 35.0% | |
| CTTGTAGAAAGAGTTTCATC+AGG | + | chr5.1:70869560-70869579 | None:intergenic | 35.0% | |
| GAATTGTGAAGGCTTTCATT+GGG | + | chr5.1:70870151-70870170 | None:intergenic | 35.0% | |
| GGGACAAAGAGTAACAAAAT+TGG | + | chr5.1:70870266-70870285 | None:intergenic | 35.0% | |
| GTCATCAAAACAACAGCTTT+AGG | - | chr5.1:70869375-70869394 | MS.gene038142:CDS | 35.0% | |
| GTCCCAATTTCACAAATTGT+TGG | - | chr5.1:70869728-70869747 | MS.gene038142:CDS | 35.0% | |
| TAGTAGAGCAAGAATTCACA+TGG | - | chr5.1:70869679-70869698 | MS.gene038142:CDS | 35.0% | |
| ! | CACATGGAAGATCAACTTTT+AGG | - | chr5.1:70869695-70869714 | MS.gene038142:CDS | 35.0% |
| !! | TCACCTTCTTTCTTTGAAGT+TGG | + | chr5.1:70870295-70870314 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTTGGTGTATC+GGG | + | chr5.1:70870286-70870305 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | - | chr5.1:70869706-70869725 | MS.gene038142:CDS | 35.0% |
| AAGATGAAGCAGAGTTCACA+GGG | - | chr5.1:70869438-70869457 | MS.gene038142:CDS | 40.0% | |
| ACCGCTATGATCTTGTCTTT+CGG | + | chr5.1:70870557-70870576 | None:intergenic | 40.0% | |
| GATAGTAGAGCTTACCAAAC+CGG | + | chr5.1:70870173-70870192 | None:intergenic | 40.0% | |
| GGAATTGTGAAGGCTTTCAT+TGG | + | chr5.1:70870152-70870171 | None:intergenic | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | + | chr5.1:70869809-70869828 | None:intergenic | 40.0% | |
| TGCTATGCAGAAGCAAATAG+TGG | - | chr5.1:70870456-70870475 | MS.gene038142:CDS | 40.0% | |
| TTACCAAACCGGAATTGTGA+AGG | + | chr5.1:70870162-70870181 | None:intergenic | 40.0% | |
| TTCATCAGGGTTGTAAGTGA+GGG | + | chr5.1:70869546-70869565 | None:intergenic | 40.0% | |
| TTTCATCAGGGTTGTAAGTG+AGG | + | chr5.1:70869547-70869566 | None:intergenic | 40.0% | |
| ! | CTTTTTCTCAAACGAGTCTG+CGG | + | chr5.1:70870221-70870240 | None:intergenic | 40.0% |
| ! | GAAGCACCAAGATGAAGAAA+TGG | + | chr5.1:70870058-70870077 | None:intergenic | 40.0% |
| ! | TTGCTGCCATTTCTTCATCT+TGG | - | chr5.1:70870049-70870068 | MS.gene038142:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | - | chr5.1:70869791-70869810 | MS.gene038142:CDS | 40.0% |
| AAGCCTTCACAATTCCGGTT+TGG | - | chr5.1:70870156-70870175 | MS.gene038142:CDS | 45.0% | |
| CCCGAAAGACAAGATCATAG+CGG | - | chr5.1:70870553-70870572 | MS.gene038142:CDS | 45.0% | |
| CCGCTATGATCTTGTCTTTC+GGG | + | chr5.1:70870556-70870575 | None:intergenic | 45.0% | |
| GAAGATGAAGCAGAGTTCAC+AGG | - | chr5.1:70869437-70869456 | MS.gene038142:CDS | 45.0% | |
| GAATCCACCTGATTCTCATC+TGG | + | chr5.1:70870121-70870140 | None:intergenic | 45.0% | |
| GCAACAGTTTACTGAGTCCT+TGG | - | chr5.1:70870498-70870517 | MS.gene038142:CDS | 45.0% | |
| GGCGAAAATGAAGCTTCCTA+TGG | - | chr5.1:70870519-70870538 | MS.gene038142:CDS | 45.0% | |
| ! | AGGAAGCTTCATTTTCGCCA+AGG | + | chr5.1:70870518-70870537 | None:intergenic | 45.0% |
| ! | GATGGTTCGCGTGTGTTTTA+TGG | - | chr5.1:70870631-70870650 | MS.gene038142:CDS | 45.0% |
| GAGTTCGCCAGATGAGAATC+AGG | - | chr5.1:70870111-70870130 | MS.gene038142:CDS | 50.0% | |
| TCGGGTTTGTCAACGTCCAT+AGG | + | chr5.1:70870538-70870557 | None:intergenic | 50.0% | |
| TTCGCCAGATGAGAATCAGG+TGG | - | chr5.1:70870114-70870133 | MS.gene038142:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 70869356 | 70870672 | 70869356 | ID=MS.gene038142 |
| chr5.1 | mRNA | 70869356 | 70870672 | 70869356 | ID=MS.gene038142.t1;Parent=MS.gene038142 |
| chr5.1 | exon | 70869356 | 70870672 | 70869356 | ID=MS.gene038142.t1.exon1;Parent=MS.gene038142.t1 |
| chr5.1 | CDS | 70869356 | 70870672 | 70869356 | ID=cds.MS.gene038142.t1;Parent=MS.gene038142.t1 |
| Gene Sequence |
| Protein sequence |
