AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023253

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023253.t1 MTR_1g112270 45.669 254 133 4 30 280 47 298 1.26e-72 238
MS.gene023253.t1 MTR_8g017210 64.103 156 54 2 27 182 62 215 6.50e-72 235
MS.gene023253.t1 MTR_2g099990 63.462 156 55 2 27 182 46 199 4.44e-71 231
MS.gene023253.t1 MTR_2g093960 62.179 156 58 1 27 182 32 186 5.49e-71 229
MS.gene023253.t1 MTR_1g016010 61.392 158 60 1 27 184 41 197 1.47e-70 229
MS.gene023253.t1 MTR_1g112270 45.528 246 129 4 38 280 66 309 2.48e-69 229
MS.gene023253.t1 MTR_1g094115 60.897 156 59 2 27 182 47 200 5.26e-69 228
MS.gene023253.t1 MTR_4g059870 60.256 156 61 1 27 182 38 192 5.52e-69 226
MS.gene023253.t1 MTR_2g090745 62.162 148 54 2 35 182 15 160 1.94e-63 210
MS.gene023253.t1 MTR_2g099990 63.636 132 46 2 51 182 1 130 7.99e-59 197
MS.gene023253.t1 MTR_1g094115 53.205 156 54 3 27 182 47 183 2.09e-54 189
MS.gene023253.t1 MTR_2g017720 54.887 133 53 2 20 152 306 431 2.31e-39 146
MS.gene023253.t1 MTR_3g102000 46.565 131 58 5 27 150 6 131 1.77e-26 106
MS.gene023253.t1 MTR_5g030560 64.444 45 16 0 37 81 16 60 2.42e-16 73.9
MS.gene023253.t1 MTR_4g103250 34.884 86 56 0 311 396 58 143 1.21e-14 71.2
MS.gene023253.t1 MTR_1g093095 27.374 179 114 6 15 181 74 248 3.13e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023253.t1 AT2G01940 57.482 421 151 9 17 421 34 442 2.03e-164 469
MS.gene023253.t1 AT2G01940 56.738 423 152 11 17 421 34 443 7.02e-159 456
MS.gene023253.t1 AT1G68130 56.863 408 137 9 21 419 34 411 2.52e-149 430
MS.gene023253.t1 AT1G25250 53.846 429 137 10 3 421 8 385 2.23e-145 419
MS.gene023253.t1 AT1G25250 54.315 394 120 9 37 421 20 362 8.48e-135 391
MS.gene023253.t1 AT2G01940 54.247 365 139 9 73 421 1 353 8.60e-127 370
MS.gene023253.t1 AT1G68130 52.113 355 132 8 73 419 1 325 1.17e-112 334
MS.gene023253.t1 AT1G25250 50.000 358 119 9 73 421 1 307 4.40e-106 316
MS.gene023253.t1 AT3G50700 56.757 185 74 3 2 182 5 187 6.74e-72 233
MS.gene023253.t1 AT5G66730 57.219 187 68 4 2 182 5 185 7.52e-71 231
MS.gene023253.t1 AT4G02670 49.569 232 95 4 27 243 55 279 3.37e-70 227
MS.gene023253.t1 AT4G02670 49.569 232 95 4 27 243 53 277 3.59e-70 227
MS.gene023253.t1 AT3G45260 55.747 174 76 1 27 200 39 211 1.08e-69 227
MS.gene023253.t1 AT3G45260 55.747 174 76 1 27 200 39 211 1.08e-69 227
MS.gene023253.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.21e-69 227
MS.gene023253.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.21e-69 227
MS.gene023253.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.21e-69 227
MS.gene023253.t1 AT3G13810 62.821 156 57 1 27 182 51 205 2.61e-69 228
MS.gene023253.t1 AT5G60470 54.098 183 83 1 27 209 44 225 3.44e-69 226
MS.gene023253.t1 AT3G13810 62.821 156 57 1 27 182 70 224 3.67e-69 228
MS.gene023253.t1 AT3G13810 62.821 156 57 1 27 182 70 224 3.67e-69 228
MS.gene023253.t1 AT3G13810 62.821 156 57 1 27 182 65 219 3.79e-69 227
MS.gene023253.t1 AT5G60470 54.098 183 83 1 27 209 40 221 4.01e-69 226
MS.gene023253.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.43e-69 228
MS.gene023253.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.43e-69 228
MS.gene023253.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.43e-69 228
MS.gene023253.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.43e-69 228
MS.gene023253.t1 AT5G03150 61.538 156 59 1 27 182 53 207 5.48e-69 227
MS.gene023253.t1 AT1G03840 63.462 156 55 2 27 182 41 194 2.51e-68 225
MS.gene023253.t1 AT1G14580 58.974 156 62 2 27 182 53 206 3.05e-68 224
MS.gene023253.t1 AT1G14580 58.974 156 62 2 27 182 53 206 3.05e-68 224
MS.gene023253.t1 AT1G14580 58.974 156 62 2 27 182 67 220 4.78e-68 224
MS.gene023253.t1 AT2G02070 61.538 156 58 2 27 182 52 205 1.18e-67 226
MS.gene023253.t1 AT2G02070 61.538 156 58 2 27 182 52 205 1.18e-67 226
MS.gene023253.t1 AT5G44160 49.302 215 97 5 27 236 37 244 2.35e-66 219
MS.gene023253.t1 AT3G13810 64.138 145 51 1 38 182 68 211 7.06e-65 216
MS.gene023253.t1 AT5G60470 54.070 172 78 1 38 209 46 216 9.12e-65 214
MS.gene023253.t1 AT3G13810 64.138 145 51 1 38 182 82 225 9.78e-65 216
MS.gene023253.t1 AT1G03840 62.179 156 55 3 27 182 41 192 1.05e-64 216
MS.gene023253.t1 AT5G44160 49.268 205 92 5 37 236 8 205 6.48e-63 209
MS.gene023253.t1 AT2G02080 53.205 156 68 3 51 203 1 154 2.19e-56 192
MS.gene023253.t1 AT2G02080 53.205 156 68 3 51 203 1 154 2.19e-56 192
MS.gene023253.t1 AT5G22890 31.138 167 101 5 25 178 186 351 7.35e-17 82.0
MS.gene023253.t1 AT1G34370 29.231 195 119 7 36 216 14 203 1.16e-16 80.5
MS.gene023253.t1 AT1G34370 29.231 195 119 7 36 216 224 413 1.67e-16 81.6
MS.gene023253.t1 AT1G34370 29.231 195 119 7 36 216 224 413 1.67e-16 81.6
MS.gene023253.t1 AT3G48550 31.034 174 87 6 226 393 11 157 1.90e-15 73.9
MS.gene023253.t1 AT3G57670 30.986 142 87 5 48 181 209 347 4.26e-12 67.8

Find 74 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATGCATTTGAAGATTC+TGG 0.162069 4.3:-17662558 None:intergenic
CAGTTTCAATCTTCAACTTC+TGG 0.249687 4.3:+17664819 MS.gene023253:CDS
AATGGTTTATCAGGTGGTTT+TGG 0.294809 4.3:-17664336 None:intergenic
TTGAGTTTGAAAATGCTAAA+AGG 0.314430 4.3:+17664685 MS.gene023253:CDS
AGAAGAAACATCCATTGTTA+TGG 0.316252 4.3:+17664851 MS.gene023253:CDS
TCTGATTCTAACAAAGTCTT+TGG 0.339052 4.3:-17662493 None:intergenic
CTAAAATTTGCCTCACTAGA+TGG 0.342793 4.3:-17664279 None:intergenic
GCACTGGTAATCCTTGTAAT+GGG 0.366655 4.3:-17664311 None:intergenic
GGTGCTGATAATGGTTTATC+AGG 0.373734 4.3:-17664345 None:intergenic
GAAGATAAGTAACACTGTTA+TGG 0.384215 4.3:+17664770 MS.gene023253:CDS
TGATGCAAACAACTTGGTTC+TGG 0.390697 4.3:-17662655 None:intergenic
GAAGAAACATCCATTGTTAT+GGG 0.391163 4.3:+17664852 MS.gene023253:CDS
ACACACTTGAAACTTTCAAT+AGG 0.397442 4.3:+17664504 MS.gene023253:CDS
ATTGTTGTTGCTTGGAACTC+TGG 0.397896 4.3:-17664216 None:intergenic
CAGGCTGCATTGTTGTTGCT+TGG 0.402771 4.3:-17664224 None:intergenic
AACAAGGTAGGTGCTGATAA+TGG 0.405416 4.3:-17664354 None:intergenic
GAAACTGTTGCTTACAAGCT+TGG 0.418931 4.3:-17664803 None:intergenic
TCTGGTCTCTTTGAAACCCT+TGG 0.440797 4.3:-17662540 None:intergenic
AGAGACACAACTTCAGCATC+TGG 0.455957 4.3:-17662466 None:intergenic
AGAGTTGAAGTTCTAAGTTA+TGG 0.456212 4.3:-17664422 None:intergenic
AAAGTTGGCAATGGCAGAAA+AGG 0.462255 4.3:+17664608 MS.gene023253:CDS
AGCACTGGTAATCCTTGTAA+TGG 0.468962 4.3:-17664312 None:intergenic
ACAATGGATGTTTCTTCTGA+TGG 0.469771 4.3:-17664846 None:intergenic
TTCCCCTTCTGTGGTGGCTG+AGG 0.471434 4.3:-17664883 None:intergenic
ATGTTACCTGGTGTGCCTGC+CGG 0.489494 4.3:-17662361 None:intergenic
AGTATATCAGCCCCATTACA+AGG 0.490202 4.3:+17664300 MS.gene023253:CDS
TTCTGCTTCCCCTTCTGTGG+TGG 0.491783 4.3:-17664889 None:intergenic
TTGTTGCTTGGAACTCTGGT+TGG 0.493027 4.3:-17664212 None:intergenic
AGAAGACCGGCAGGCACACC+AGG 0.497770 4.3:+17662355 MS.gene023253:CDS
ACATAGTAATCATAAACAAT+GGG 0.505280 4.3:+17662735 MS.gene023253:CDS
GTTTGTGAGATCTGTAACCA+AGG 0.507755 4.3:+17662523 MS.gene023253:CDS
TACTAGTGAGGAGCTAAAGT+TGG 0.509569 4.3:+17664593 MS.gene023253:CDS
AAACAAGCACAAGATGAACT+TGG 0.527243 4.3:+17664714 MS.gene023253:CDS
ACTTGAGGGTTTATAGAGGA+AGG 0.528633 4.3:-17664447 None:intergenic
TCATTCTGCTTCCCCTTCTG+TGG 0.531823 4.3:-17664892 None:intergenic
AACATAGTAATCATAAACAA+TGG 0.531879 4.3:+17662734 MS.gene023253:CDS
ATTAACTGTGTTATCTAACA+TGG 0.533403 4.3:-17662266 None:intergenic
TTTGAAAGAGTTTACTAGTG+AGG 0.537749 4.3:+17664581 MS.gene023253:CDS
CATGATCCATGTCATGCACT+AGG 0.544901 4.3:+17662676 MS.gene023253:CDS
TTCTACTTGAGGGTTTATAG+AGG 0.552798 4.3:-17664451 None:intergenic
TTTGTGAGATCTGTAACCAA+GGG 0.553552 4.3:+17662524 MS.gene023253:CDS
GCTGATAATGGTTTATCAGG+TGG 0.558635 4.3:-17664342 None:intergenic
GTTGAAGTTCTAAGTTATGG+TGG 0.560774 4.3:-17664419 None:intergenic
ATGTCCTCAGCCACCACAGA+AGG 0.565952 4.3:+17664879 MS.gene023253:CDS
GGATCATGATGCAAACAACT+TGG 0.569323 4.3:-17662661 None:intergenic
AGAGGACATTCGTGTGATTG+TGG 0.573870 4.3:+17662829 MS.gene023253:CDS
CATGCACTAGGTGACCTTGT+TGG 0.575089 4.3:+17662688 MS.gene023253:CDS
GTTATGGTGGTGATGAGAAG+TGG 0.575098 4.3:-17664406 None:intergenic
ACAATCACACGAATGTCCTC+TGG 0.575624 4.3:-17662827 None:intergenic
TGATAATGCAAAAGCAGTAG+TGG 0.577782 4.3:-17662302 None:intergenic
TGTCCTCAGCCACCACAGAA+GGG 0.586753 4.3:+17664880 MS.gene023253:CDS
GTTTGTGACAAATGCAACAA+AGG 0.587309 4.3:+17662757 MS.gene023253:CDS
GAAGAAGACACAAAGTGCCA+TGG 0.587753 4.3:+17662578 MS.gene023253:CDS
TCTGAATTAGTTAACAAGGT+AGG 0.590831 4.3:-17664366 None:intergenic
AGCAGTAGTGGCATCAGAAG+AGG 0.593577 4.3:-17662290 None:intergenic
ATAATGAGTCAGAGAAACAA+AGG 0.608502 4.3:+17664529 MS.gene023253:CDS
CAAACTTGCTAGTTCCAATG+TGG 0.610019 4.3:-17664562 None:intergenic
AAGGAATGATGTATCCACAT+TGG 0.613625 4.3:+17664548 MS.gene023253:CDS
GTCCTCAGCCACCACAGAAG+GGG 0.615788 4.3:+17664881 MS.gene023253:CDS
TGAGGAGCTAAAGTTGGCAA+TGG 0.615902 4.3:+17664599 MS.gene023253:CDS
TTAACTGTGTTATCTAACAT+GGG 0.627719 4.3:-17662265 None:intergenic
CAAGACGCATGCACAATCAG+AGG 0.629083 4.3:+17664183 MS.gene023253:CDS
ATCAAAACATGTGGTACCAG+AGG 0.630022 4.3:+17662811 MS.gene023253:CDS
TGTATCTGAATTAGTTAACA+AGG 0.634479 4.3:-17664370 None:intergenic
GCAGTTCTAGAAGAACATGC+AGG 0.635627 4.3:-17664243 None:intergenic
CACTGGTAATCCTTGTAATG+GGG 0.639858 4.3:-17664310 None:intergenic
TGGTGGTGATGAGAAGTGGA+AGG 0.646826 4.3:-17664402 None:intergenic
AAAGCACATATCAAAACATG+TGG 0.657146 4.3:+17662802 MS.gene023253:CDS
AAGCACAAGTCCATCTAGTG+AGG 0.660327 4.3:+17664269 MS.gene023253:CDS
AATGTTTCTTGATTCCAACA+AGG 0.660532 4.3:-17662702 None:intergenic
AGGTCACCTAGTGCATGACA+TGG 0.667882 4.3:-17662682 None:intergenic
GGACATGTAACCCATAACAA+TGG 0.669538 4.3:-17664862 None:intergenic
TTTGCATTATCAGAAAATGG+GGG 0.689769 4.3:+17662313 MS.gene023253:CDS
TTGAAAAGAGAAACAACACA+AGG 0.699162 4.3:+17662607 MS.gene023253:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTAGTTATTAATATATAT+AGG + chr4.3:17663761-17663780 MS.gene023253:intron 10.0%
!! GTAAAATACTAATATTAATT+TGG - chr4.3:17663089-17663108 None:intergenic 10.0%
!!! ATATTTAAATAGTACTATTT+TGG + chr4.3:17663280-17663299 MS.gene023253:intron 10.0%
!! AAATTAATTAATCAAAGATC+TGG - chr4.3:17663646-17663665 None:intergenic 15.0%
!! AATTAATTAATCAAAGATCT+GGG - chr4.3:17663645-17663664 None:intergenic 15.0%
!! TAGCTAATATTATAAGTTTA+TGG + chr4.3:17663454-17663473 MS.gene023253:intron 15.0%
!! TTTCATCTTAAAAAATAACA+TGG - chr4.3:17663693-17663712 None:intergenic 15.0%
!!! ACTCTCAAATATAAAATTTT+AGG - chr4.3:17663179-17663198 None:intergenic 15.0%
!!! CTCTCAAATATAAAATTTTA+GGG - chr4.3:17663178-17663197 None:intergenic 15.0%
!! AACATAGTAATCATAAACAA+TGG + chr4.3:17662734-17662753 MS.gene023253:CDS 20.0%
!! AATTAAATTCTCATCTTCTT+AGG + chr4.3:17663238-17663257 MS.gene023253:intron 20.0%
!! ACATAGTAATCATAAACAAT+GGG + chr4.3:17662735-17662754 MS.gene023253:CDS 20.0%
!! AGTCAATAACCATATATTAT+AGG - chr4.3:17664115-17664134 None:intergenic 20.0%
!! ATGAGAATTTAATTGCAAAA+AGG - chr4.3:17663232-17663251 None:intergenic 20.0%
!! TGAGAATTTAATTGCAAAAA+GGG - chr4.3:17663231-17663250 None:intergenic 20.0%
!!! ATTTTTTAACCTTGTTTTGT+GGG + chr4.3:17662432-17662451 MS.gene023253:intron 20.0%
!!! GTCAAATTATATTTTCATAC+AGG + chr4.3:17663926-17663945 MS.gene023253:intron 20.0%
!!! TATTTTTTAACCTTGTTTTG+TGG + chr4.3:17662431-17662450 MS.gene023253:intron 20.0%
!!! TTTTATCAATAAACCAAACA+AGG + chr4.3:17663501-17663520 MS.gene023253:intron 20.0%
! ACTATCTACTACAATAAAGA+TGG - chr4.3:17662932-17662951 None:intergenic 25.0%
! ATAAGACAGAATGATCAAAT+AGG - chr4.3:17663580-17663599 None:intergenic 25.0%
! TAACAAAAGAAAAAGAAGAC+CGG + chr4.3:17662342-17662361 MS.gene023253:CDS 25.0%
! TAAGACAGAATGATCAAATA+GGG - chr4.3:17663579-17663598 None:intergenic 25.0%
! TAAGATGAAATAGCTATAAC+AGG + chr4.3:17663703-17663722 MS.gene023253:intron 25.0%
! TAATATATATAGGCCTTGAT+TGG + chr4.3:17663771-17663790 MS.gene023253:intron 25.0%
! TACAATAAAGATGGAAAACT+TGG - chr4.3:17662923-17662942 None:intergenic 25.0%
! TAGTAGCTAATTAATGATAC+TGG - chr4.3:17663432-17663451 None:intergenic 25.0%
! TATTGATAAAAAACGCAGAT+AGG - chr4.3:17663492-17663511 None:intergenic 25.0%
! TGTATCTGAATTAGTTAACA+AGG - chr4.3:17664373-17664392 None:intergenic 25.0%
! TTGAGTTTGAAAATGCTAAA+AGG + chr4.3:17664685-17664704 MS.gene023253:CDS 25.0%
!! CTTTTGCATTATCAGAAAAT+GGG + chr4.3:17662311-17662330 MS.gene023253:CDS 25.0%
!! TTTTCTCTCTCTTTCTTTTT+CGG + chr4.3:17662963-17662982 MS.gene023253:intron 25.0%
!! TTTTGCATTATCAGAAAATG+GGG + chr4.3:17662312-17662331 MS.gene023253:CDS 25.0%
!!! AATTTGGTTTTTCTACTTGA+GGG - chr4.3:17664464-17664483 None:intergenic 25.0%
!!! ATTCATTTTAGTACCTTGTT+TGG - chr4.3:17663517-17663536 None:intergenic 25.0%
!!! TAGTTTTGATCGTTTGAATT+TGG - chr4.3:17664480-17664499 None:intergenic 25.0%
AAAGCACATATCAAAACATG+TGG + chr4.3:17662802-17662821 MS.gene023253:CDS 30.0%
AATGTTTCTTGATTCCAACA+AGG - chr4.3:17662705-17662724 None:intergenic 30.0%
ACAAACTGTTTATCCAATCA+AGG - chr4.3:17663787-17663806 None:intergenic 30.0%
ACACACTTGAAACTTTCAAT+AGG + chr4.3:17664504-17664523 MS.gene023253:CDS 30.0%
AGAAGAAACATCCATTGTTA+TGG + chr4.3:17664851-17664870 MS.gene023253:CDS 30.0%
ATAATGAGTCAGAGAAACAA+AGG + chr4.3:17664529-17664548 MS.gene023253:CDS 30.0%
CAATATGAGAAAGTGAAAGA+TGG - chr4.3:17662891-17662910 None:intergenic 30.0%
CCAAACAGACCTATAATATA+TGG + chr4.3:17664103-17664122 MS.gene023253:intron 30.0%
CCATATATTATAGGTCTGTT+TGG - chr4.3:17664106-17664125 None:intergenic 30.0%
GAAGAAACATCCATTGTTAT+GGG + chr4.3:17664852-17664871 MS.gene023253:CDS 30.0%
GAAGATAAGTAACACTGTTA+TGG + chr4.3:17664770-17664789 MS.gene023253:CDS 30.0%
GTTCATCATTCATCAAATTC+AGG + chr4.3:17663024-17663043 MS.gene023253:intron 30.0%
TCTGAATTAGTTAACAAGGT+AGG - chr4.3:17664369-17664388 None:intergenic 30.0%
TCTGATTCTAACAAAGTCTT+TGG - chr4.3:17662496-17662515 None:intergenic 30.0%
TTACATTTGCCCTTTGATTA+GGG + chr4.3:17664140-17664159 MS.gene023253:intron 30.0%
TTCTATGCATTTGAAGATTC+TGG - chr4.3:17662561-17662580 None:intergenic 30.0%
TTGAAAAGAGAAACAACACA+AGG + chr4.3:17662607-17662626 MS.gene023253:CDS 30.0%
TTTGAAAGAGTTTACTAGTG+AGG + chr4.3:17664581-17664600 MS.gene023253:CDS 30.0%
TTTGCATTATCAGAAAATGG+GGG + chr4.3:17662313-17662332 MS.gene023253:CDS 30.0%
! AATTTTGCTGATATGTTACC+TGG - chr4.3:17662376-17662395 None:intergenic 30.0%
! CAATTGACTGTGTTTGTTAA+CGG + chr4.3:17663060-17663079 MS.gene023253:intron 30.0%
! GCTTTTGCATTATCAGAAAA+TGG + chr4.3:17662310-17662329 MS.gene023253:CDS 30.0%
! TCTCTTTTCAACAATTTCCA+TGG - chr4.3:17662598-17662617 None:intergenic 30.0%
! TTTTTACATGCTCTCTCAAA+CGG + chr4.3:17664036-17664055 MS.gene023253:intron 30.0%
!! AGAGTTGAAGTTCTAAGTTA+TGG - chr4.3:17664425-17664444 None:intergenic 30.0%
!! GAAGACTTAATTTGTACTAG+TGG + chr4.3:17663550-17663569 MS.gene023253:intron 30.0%
!!! GAATTTGGTTTTTCTACTTG+AGG - chr4.3:17664465-17664484 None:intergenic 30.0%
AAACAAGCACAAGATGAACT+TGG + chr4.3:17664714-17664733 MS.gene023253:CDS 35.0%
AAGGAATGATGTATCCACAT+TGG + chr4.3:17664548-17664567 MS.gene023253:CDS 35.0%
ACAATGGATGTTTCTTCTGA+TGG - chr4.3:17664849-17664868 None:intergenic 35.0%
CAGTTTCAATCTTCAACTTC+TGG + chr4.3:17664819-17664838 MS.gene023253:CDS 35.0%
CGTTAACAAACACAGTCAAT+TGG - chr4.3:17663062-17663081 None:intergenic 35.0%
CTAAAATTTGCCTCACTAGA+TGG - chr4.3:17664282-17664301 None:intergenic 35.0%
GTTACATTTGCCCTTTGATT+AGG + chr4.3:17664139-17664158 MS.gene023253:intron 35.0%
GTTTGTGACAAATGCAACAA+AGG + chr4.3:17662757-17662776 MS.gene023253:CDS 35.0%
TTCTACTTGAGGGTTTATAG+AGG - chr4.3:17664454-17664473 None:intergenic 35.0%
TTTGTGAGATCTGTAACCAA+GGG + chr4.3:17662524-17662543 MS.gene023253:CDS 35.0%
! AATGGTTTATCAGGTGGTTT+TGG - chr4.3:17664339-17664358 None:intergenic 35.0%
! TGATAATGCAAAAGCAGTAG+TGG - chr4.3:17662305-17662324 None:intergenic 35.0%
!! GTTGAAGTTCTAAGTTATGG+TGG - chr4.3:17664422-17664441 None:intergenic 35.0%
!! TTAATGATACTGGCTCTTAC+AGG - chr4.3:17663422-17663441 None:intergenic 35.0%
!!! ATTTTAGGGTTTGACTTCCT+AGG - chr4.3:17663164-17663183 None:intergenic 35.0%
AAAAGAAAAAGAAGACCGGC+AGG + chr4.3:17662346-17662365 MS.gene023253:CDS 40.0%
AAACTCTCCACCCTAATCAA+AGG - chr4.3:17664153-17664172 None:intergenic 40.0%
AAAGCCTGCATAATGATGAG+GGG - chr4.3:17663391-17663410 None:intergenic 40.0%
AAAGTTGGCAATGGCAGAAA+AGG + chr4.3:17664608-17664627 MS.gene023253:CDS 40.0%
AACTCTCCACCCTAATCAAA+GGG - chr4.3:17664152-17664171 None:intergenic 40.0%
AAGAAAGCAGAACACAAACC+TGG - chr4.3:17662866-17662885 None:intergenic 40.0%
ACAGTTCTTAGGACTGTAGT+AGG + chr4.3:17664063-17664082 MS.gene023253:intron 40.0%
ACTTGAGGGTTTATAGAGGA+AGG - chr4.3:17664450-17664469 None:intergenic 40.0%
AGTATATCAGCCCCATTACA+AGG + chr4.3:17664300-17664319 MS.gene023253:CDS 40.0%
ATCAAAACATGTGGTACCAG+AGG + chr4.3:17662811-17662830 MS.gene023253:CDS 40.0%
CAAACGGTTCAACAGTTCTT+AGG + chr4.3:17664052-17664071 MS.gene023253:intron 40.0%
CAAAGCCTGCATAATGATGA+GGG - chr4.3:17663392-17663411 None:intergenic 40.0%
CACTGGTAATCCTTGTAATG+GGG - chr4.3:17664313-17664332 None:intergenic 40.0%
CTAAACACACCCACAAAACA+AGG - chr4.3:17662444-17662463 None:intergenic 40.0%
GGACATGTAACCCATAACAA+TGG - chr4.3:17664865-17664884 None:intergenic 40.0%
GGATCATGATGCAAACAACT+TGG - chr4.3:17662664-17662683 None:intergenic 40.0%
GTTTGTGAGATCTGTAACCA+AGG + chr4.3:17662523-17662542 MS.gene023253:CDS 40.0%
TACTAGTGAGGAGCTAAAGT+TGG + chr4.3:17664593-17664612 MS.gene023253:CDS 40.0%
TGATGCAAACAACTTGGTTC+TGG - chr4.3:17662658-17662677 None:intergenic 40.0%
! ATTGTTGTTGCTTGGAACTC+TGG - chr4.3:17664219-17664238 None:intergenic 40.0%
! GAAACTGTTGCTTACAAGCT+TGG - chr4.3:17664806-17664825 None:intergenic 40.0%
! GCACTGGTAATCCTTGTAAT+GGG - chr4.3:17664314-17664333 None:intergenic 40.0%
!! AACAAGGTAGGTGCTGATAA+TGG - chr4.3:17664357-17664376 None:intergenic 40.0%
!! AGCACTGGTAATCCTTGTAA+TGG - chr4.3:17664315-17664334 None:intergenic 40.0%
!! CAAACTTGCTAGTTCCAATG+TGG - chr4.3:17664565-17664584 None:intergenic 40.0%
!! GCTGATAATGGTTTATCAGG+TGG - chr4.3:17664345-17664364 None:intergenic 40.0%
!! GGTGCTGATAATGGTTTATC+AGG - chr4.3:17664348-17664367 None:intergenic 40.0%
AAGATCTGGGTCATGACTCA+TGG - chr4.3:17663632-17663651 None:intergenic 45.0%
AAGCACAAGTCCATCTAGTG+AGG + chr4.3:17664269-17664288 MS.gene023253:CDS 45.0%
AAGCCTGCATAATGATGAGG+GGG - chr4.3:17663390-17663409 None:intergenic 45.0%
ACAATCACACGAATGTCCTC+TGG - chr4.3:17662830-17662849 None:intergenic 45.0%
AGAGACACAACTTCAGCATC+TGG - chr4.3:17662469-17662488 None:intergenic 45.0%
AGAGGACATTCGTGTGATTG+TGG + chr4.3:17662829-17662848 MS.gene023253:CDS 45.0%
AGATCTGGGTCATGACTCAT+GGG - chr4.3:17663631-17663650 None:intergenic 45.0%
CATGATCCATGTCATGCACT+AGG + chr4.3:17662676-17662695 MS.gene023253:CDS 45.0%
CATTTGCCCTTTGATTAGGG+TGG + chr4.3:17664143-17664162 MS.gene023253:intron 45.0%
GCAAAGCCTGCATAATGATG+AGG - chr4.3:17663393-17663412 None:intergenic 45.0%
TCTTCTGCAGCTATCTTCCT+AGG + chr4.3:17663144-17663163 MS.gene023253:intron 45.0%
TGAGGAGCTAAAGTTGGCAA+TGG + chr4.3:17664599-17664618 MS.gene023253:CDS 45.0%
! ATTGTGGTCGTGTGTTTTCC+AGG + chr4.3:17662845-17662864 MS.gene023253:CDS 45.0%
! GAAGAAGACACAAAGTGCCA+TGG + chr4.3:17662578-17662597 MS.gene023253:CDS 45.0%
! GTTATGGTGGTGATGAGAAG+TGG - chr4.3:17664409-17664428 None:intergenic 45.0%
! TTGTTGCTTGGAACTCTGGT+TGG - chr4.3:17664215-17664234 None:intergenic 45.0%
!! GCAGTTCTAGAAGAACATGC+AGG - chr4.3:17664246-17664265 None:intergenic 45.0%
!! TCTGGTCTCTTTGAAACCCT+TGG - chr4.3:17662543-17662562 None:intergenic 45.0%
AGCAGTAGTGGCATCAGAAG+AGG - chr4.3:17662293-17662312 None:intergenic 50.0%
AGGTCACCTAGTGCATGACA+TGG - chr4.3:17662685-17662704 None:intergenic 50.0%
ATAATGATGAGGGGGTGGGT+GGG - chr4.3:17663382-17663401 None:intergenic 50.0%
CAAGACGCATGCACAATCAG+AGG + chr4.3:17664183-17664202 MS.gene023253:CDS 50.0%
CATGCACTAGGTGACCTTGT+TGG + chr4.3:17662688-17662707 MS.gene023253:CDS 50.0%
CTGCATAATGATGAGGGGGT+GGG - chr4.3:17663386-17663405 None:intergenic 50.0%
TCATTCTGCTTCCCCTTCTG+TGG - chr4.3:17664895-17664914 None:intergenic 50.0%
TGAGTGAGTGAGTGTGTGTG+TGG + chr4.3:17663002-17663021 MS.gene023253:intron 50.0%
! CAGGCTGCATTGTTGTTGCT+TGG - chr4.3:17664227-17664246 None:intergenic 50.0%
! TGGTGGTGATGAGAAGTGGA+AGG - chr4.3:17664405-17664424 None:intergenic 50.0%
!!! TCAGGTGGTTTTGGAAGCAC+TGG - chr4.3:17664330-17664349 None:intergenic 50.0%
ATGTCCTCAGCCACCACAGA+AGG + chr4.3:17664879-17664898 MS.gene023253:CDS 55.0%
ATGTTACCTGGTGTGCCTGC+CGG - chr4.3:17662364-17662383 None:intergenic 55.0%
CATAATGATGAGGGGGTGGG+TGG - chr4.3:17663383-17663402 None:intergenic 55.0%
CCACCCCCTCATCATTATGC+AGG + chr4.3:17663384-17663403 MS.gene023253:intron 55.0%
CCTGCATAATGATGAGGGGG+TGG - chr4.3:17663387-17663406 None:intergenic 55.0%
TGTCCTCAGCCACCACAGAA+GGG + chr4.3:17664880-17664899 MS.gene023253:CDS 55.0%
TTCTGCTTCCCCTTCTGTGG+TGG - chr4.3:17664892-17664911 None:intergenic 55.0%
GTCCTCAGCCACCACAGAAG+GGG + chr4.3:17664881-17664900 MS.gene023253:CDS 60.0%
TTCCCCTTCTGTGGTGGCTG+AGG - chr4.3:17664886-17664905 None:intergenic 60.0%
AGAAGACCGGCAGGCACACC+AGG + chr4.3:17662355-17662374 MS.gene023253:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 17662268 17664914 17662268 ID=MS.gene023253
chr4.3 mRNA 17662268 17664914 17662268 ID=MS.gene023253.t1;Parent=MS.gene023253
chr4.3 exon 17662268 17662376 17662268 ID=MS.gene023253.t1.exon1;Parent=MS.gene023253.t1
chr4.3 CDS 17662268 17662376 17662268 ID=cds.MS.gene023253.t1;Parent=MS.gene023253.t1
chr4.3 exon 17662464 17662866 17662464 ID=MS.gene023253.t1.exon2;Parent=MS.gene023253.t1
chr4.3 CDS 17662464 17662866 17662464 ID=cds.MS.gene023253.t1;Parent=MS.gene023253.t1
chr4.3 exon 17664161 17664914 17664161 ID=MS.gene023253.t1.exon3;Parent=MS.gene023253.t1
chr4.3 CDS 17664161 17664914 17664161 ID=cds.MS.gene023253.t1;Parent=MS.gene023253.t1
Gene Sequence

>MS.gene023253

ATGTTAGATAACACAGTTAATTCCTCTTCTGATGCCACTACTGCTTTTGCATTATCAGAAAATGGGGGTGCTAATAACAAAAGAAAAAGAAGACCGGCAGGCACACCAGGTAACATATCAGCAAAATTAAGATTACTTCTTTCTAACTTAGTTTTTTATTTTTTATTTTTTAACCTTGTTTTGTGGGTGTGTTTAGATCCAGATGCTGAAGTTGTGTCTCTATCACCAAAGACTTTGTTAGAATCAGACAGATATGTTTGTGAGATCTGTAACCAAGGGTTTCAAAGAGACCAGAATCTTCAAATGCATAGAAGAAGACACAAAGTGCCATGGAAATTGTTGAAAAGAGAAACAACACAAGGATTAAAAAAGAGAGTATTTGTTTGTCCAGAACCAAGTTGTTTGCATCATGATCCATGTCATGCACTAGGTGACCTTGTTGGAATCAAGAAACATTTTAGAAGAAAACATAGTAATCATAAACAATGGGTTTGTGACAAATGCAACAAAGGTTATGCTGTTCAATCTGATTATAAAGCACATATCAAAACATGTGGTACCAGAGGACATTCGTGTGATTGTGGTCGTGTGTTTTCCAGGTTTGTGTTCTGCTTTCTTTTCCATCTTTCACTTTCTCATATTGTTCTTCATTCCAAGTTTTCCATCTTTATTGTAGTAGATAGTTTCTTTTCTCTTTTTCTCTCTCTTTCTTTTTCGGTGTGTGTGTGCGTGTGTGAGTGAGTGAGTGTGTGTGTGGTTCATCATTCATCAAATTCAGGTCACTGCTGAAACCAATTGACTGTGTTTGTTAACGGAAACCAAATTAATATTAGTATTTTACTACTTCAATTATACTATATTATATTATTTGATTATTCTTCTGCAGCTATCTTCCTAGGAAGTCAAACCCTAAAATTTTATATTTGAGAGTGAAACAAAAATTCTTTTAAATCTCAGATACCCTTTTTGCAATTAAATTCTCATCTTCTTAGGTTATCATATTATTCTTCATATATTTAAATAGTACTATTTTGGAAAGTTAAATACATAAGCTGAAAATTTTAACATTATGTTTTGAAATTTTGAATATATGATATGATTATGAATACAGCCCACCCACCCCCTCATCATTATGCAGGCTTTGCAAATTGCCTGTAAGAGCCAGTATCATTAATTAGCTACTAGCTAGCTAATATTATAAGTTTATGGACTGTATTATTACCTATCTGCGTTTTTTATCAATAAACCAAACAAGGTACTAAAATGAATCATATGTAAGAAAGAAGACTTAATTTGTACTAGTGGAGACCCTATTTGATCATTCTGTCTTATTTAATTAATTAATTAATTAATTACTTTTCCCATGAGTCATGACCCAGATCTTTGATTAATTAATTTTTTTATAAACATATCTTTGTTTGACCATGTTATTTTTTAAGATGAAATAGCTATAACAGGCATTAACATATATAATATCACAATCTAGTCATATAATCTAGTTATTAATATATATAGGCCTTGATTGGATAAACAGTTTGTTGAATTATGTGATTATATAACATAGATGCTTATTATTCATGTGTTTATGTAGAAACTTTTTTTATAAAATGAAATAAAGTCAAATTATGTATAAACTTTTTTTTATAAGATAAAATAAAGTCAAATTATATTTTCATACAGGATATACTTAATCTCTTTTCAAAACTATCATCATGATGAACTTCATGAACACACTTAAAACAGTTTATAGACATGTTATTATAAGTTATTTTTACATGCTCTCTCAAACGGTTCAACAGTTCTTAGGACTGTAGTAGGTAAACTCATAAATAAATCCAAACAGACCTATAATATATGGTTATTGACTTAAAGTTACATTTGCCCTTTGATTAGGGTGGAGAGTTTCATAGAACATCAAGACGCATGCACAATCAGAGGAAAGCACCAACCAGAGTTCCAAGCAACAACAATGCAGCCTGCATGTTCTTCTAGAACTGCTTCAAGCACAAGTCCATCTAGTGAGGCAAATTTTAGTATATCAGCCCCATTACAAGGATTACCAGTGCTTCCAAAACCACCTGATAAACCATTATCAGCACCTACCTTGTTAACTAATTCAGATACACATAACAAGCCTTCCACTTCTCATCACCACCATAACTTAGAACTTCAACTCTTACCTTCCTCTATAAACCCTCAAGTAGAAAAACCAAATTCAAACGATCAAAACTACGAAACACACTTGAAACTTTCAATAGGAAATAATGAGTCAGAGAAACAAAGGAATGATGTATCCACATTGGAACTAGCAAGTTTGAAAGAGTTTACTAGTGAGGAGCTAAAGTTGGCAATGGCAGAAAAGGCTTATGCAGAAGAAGCTAGAAAAGAAGCTAAACGTCAAATTGAGATAGCTGAAGTTGAGTTTGAAAATGCTAAAAGGATAAGAAAACAAGCACAAGATGAACTTGGAAAAGCTGAAGCTTTGAGAAAACAAGCTATTAAGAAGATAAGTAACACTGTTATGGAAGTAACATGCCAAGCTTGTAAGCAACAGTTTCAATCTTCAACTTCTGGTGTTCCATCAGAAGAAACATCCATTGTTATGGGTTACATGTCCTCAGCCACCACAGAAGGGGAAGCAGAATGA
Protein sequence

>MS.gene023253.t1

MLDNTVNSSSDATTAFALSENGGANNKRKRRPAGTPDPDAEVVSLSPKTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRETTQGLKKRVFVCPEPSCLHHDPCHALGDLVGIKKHFRRKHSNHKQWVCDKCNKGYAVQSDYKAHIKTCGTRGHSCDCGRVFSRVESFIEHQDACTIRGKHQPEFQATTMQPACSSRTASSTSPSSEANFSISAPLQGLPVLPKPPDKPLSAPTLLTNSDTHNKPSTSHHHHNLELQLLPSSINPQVEKPNSNDQNYETHLKLSIGNNESEKQRNDVSTLELASLKEFTSEELKLAMAEKAYAEEARKEAKRQIEIAEVEFENAKRIRKQAQDELGKAEALRKQAIKKISNTVMEVTCQACKQQFQSSTSGVPSEETSIVMGYMSSATTEGEAE