AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02469

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02469.t1 MTR_4g086600 98.789 413 5 0 1 413 42 454 0.0 852
MS.gene02469.t1 MTR_1g020080 55.637 408 147 6 1 407 36 410 3.18e-148 427
MS.gene02469.t1 MTR_3g101370 53.623 414 138 8 1 407 42 408 4.90e-144 416
MS.gene02469.t1 MTR_4g131970 52.105 380 122 7 36 403 57 388 1.10e-129 380
MS.gene02469.t1 MTR_2g022950 58.228 316 113 4 60 374 45 342 2.22e-126 371
MS.gene02469.t1 MTR_2g097960 53.295 349 142 6 64 398 6 347 1.83e-122 362
MS.gene02469.t1 MTR_1g020080 53.667 300 106 5 1 300 36 302 1.91e-99 299
MS.gene02469.t1 MTR_3g101370 50.667 300 96 7 1 295 42 294 2.46e-93 283
MS.gene02469.t1 MTR_2g022950 60.000 210 79 2 60 268 45 250 4.95e-85 261
MS.gene02469.t1 MTR_2g022950 63.590 195 66 2 60 253 45 235 2.37e-84 259
MS.gene02469.t1 MTR_3g109090 33.333 303 173 10 74 366 43 326 1.25e-37 141
MS.gene02469.t1 MTR_3g109090 33.333 303 173 10 74 366 59 342 1.54e-37 141
MS.gene02469.t1 MTR_4g124940 30.640 297 188 8 74 366 45 327 7.31e-36 136
MS.gene02469.t1 MTR_8g022050 29.851 335 199 11 37 366 41 344 2.53e-34 132
MS.gene02469.t1 MTR_7g102730 27.119 295 157 9 76 358 11 259 3.24e-26 111
MS.gene02469.t1 MTR_7g102730 28.920 287 165 10 74 350 97 354 2.14e-22 100
MS.gene02469.t1 MTR_4g085540 27.869 305 183 9 77 372 124 400 1.04e-25 110
MS.gene02469.t1 MTR_4g085540 27.465 284 160 10 76 353 35 278 3.89e-21 96.3
MS.gene02469.t1 MTR_4g085540 26.500 200 114 6 73 254 211 395 5.42e-13 71.2
MS.gene02469.t1 MTR_1g019150 25.962 312 181 9 77 372 122 399 1.13e-20 94.7
MS.gene02469.t1 MTR_1g019150 26.148 283 165 8 76 353 33 276 1.06e-19 91.7
MS.gene02469.t1 MTR_1g019150 29.703 202 128 6 62 254 198 394 7.80e-16 80.1
MS.gene02469.t1 MTR_1g097440 26.797 306 175 11 76 374 31 294 7.17e-20 92.4
MS.gene02469.t1 MTR_1g097440 25.952 289 182 10 77 358 120 383 3.51e-19 90.1
MS.gene02469.t1 MTR_1g097440 28.495 186 121 6 77 254 211 392 5.43e-13 71.2
MS.gene02469.t1 MTR_3g102040 27.083 288 164 9 70 350 17 265 1.03e-19 91.7
MS.gene02469.t1 MTR_3g102040 24.756 307 190 10 77 372 114 390 9.00e-17 82.8
MS.gene02469.t1 MTR_3g102040 27.861 201 136 5 60 254 188 385 1.73e-13 72.8
MS.gene02469.t1 MTR_3g065190 29.670 182 117 3 76 257 26 196 1.18e-19 90.5
MS.gene02469.t1 MTR_4g104470 32.275 189 119 4 71 256 118 300 2.96e-19 88.2
MS.gene02469.t1 MTR_3g075500 33.333 195 118 5 69 258 103 290 3.57e-19 87.4
MS.gene02469.t1 MTR_1g061870 23.711 291 178 7 76 361 16 267 5.03e-19 89.4
MS.gene02469.t1 MTR_1g061870 26.481 287 168 10 77 353 105 358 4.10e-17 83.6
MS.gene02469.t1 MTR_6g040190 24.832 298 175 7 73 365 23 276 5.92e-19 89.4
MS.gene02469.t1 MTR_6g040190 27.053 207 136 5 79 276 207 407 6.88e-11 64.7
MS.gene02469.t1 MTR_1g056520 28.962 183 124 3 75 254 108 287 8.52e-18 84.0
MS.gene02469.t1 MTR_3g075500 33.908 174 103 5 69 237 103 269 9.12e-17 80.1
MS.gene02469.t1 MTR_7g106440 26.442 208 137 4 62 259 96 297 3.61e-16 79.3
MS.gene02469.t1 MTR_1g045510 29.474 190 125 5 67 255 104 285 1.96e-15 76.6
MS.gene02469.t1 MTR_3g055990 28.713 202 118 7 73 261 4 192 1.16e-13 72.8
MS.gene02469.t1 MTR_3g055990 28.713 202 118 7 73 261 4 192 1.25e-13 72.8
MS.gene02469.t1 MTR_1g080150 40.789 76 44 1 172 247 131 205 2.52e-13 69.7
MS.gene02469.t1 MTR_1g064230 28.571 196 123 3 77 256 86 280 6.32e-13 69.3
MS.gene02469.t1 MTR_5g097580 28.788 198 115 7 77 261 8 192 2.03e-12 68.9
MS.gene02469.t1 MTR_5g097580 28.788 198 115 7 77 261 8 192 3.17e-12 68.6
MS.gene02469.t1 MTR_7g118120 18.994 358 231 8 28 369 47 361 3.92e-12 68.2
MS.gene02469.t1 MTR_5g017280 25.507 345 205 10 86 393 15 344 4.05e-12 67.8
MS.gene02469.t1 MTR_6g004810 22.549 204 145 5 58 259 26 218 6.33e-12 67.0
MS.gene02469.t1 MTR_5g017280 25.507 345 205 10 86 393 15 344 7.26e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02469.t1 AT3G19130 63.636 385 104 8 2 375 43 402 5.14e-164 468
MS.gene02469.t1 AT1G49600 66.366 333 96 6 72 403 116 433 3.86e-154 443
MS.gene02469.t1 AT1G49600 68.475 295 83 2 72 366 116 400 2.70e-146 423
MS.gene02469.t1 AT1G49600 68.475 295 83 2 72 366 116 400 2.70e-146 423
MS.gene02469.t1 AT1G47490 56.522 391 137 9 30 407 48 418 6.43e-143 414
MS.gene02469.t1 AT1G47500 59.167 360 124 7 51 407 81 420 7.28e-139 404
MS.gene02469.t1 AT1G11650 54.683 331 123 6 72 400 59 364 1.86e-120 356
MS.gene02469.t1 AT5G19350 49.176 364 153 5 72 412 21 375 2.63e-118 351
MS.gene02469.t1 AT5G19350 49.315 365 155 5 72 412 21 379 1.03e-117 350
MS.gene02469.t1 AT5G54900 50.882 340 147 3 58 396 43 363 1.91e-115 342
MS.gene02469.t1 AT4G27000 56.436 303 110 4 73 374 78 359 1.12e-113 339
MS.gene02469.t1 AT1G11650 56.134 269 95 5 72 338 59 306 4.38e-103 308
MS.gene02469.t1 AT1G47490 53.929 280 98 7 30 297 48 308 4.41e-92 280
MS.gene02469.t1 AT1G47500 57.258 248 85 5 51 296 81 309 5.48e-87 267
MS.gene02469.t1 AT1G54080 31.579 361 201 12 11 366 26 345 3.32e-43 156
MS.gene02469.t1 AT1G54080 31.233 365 201 13 11 366 26 349 1.53e-41 152
MS.gene02469.t1 AT3G14100 33.555 301 181 9 71 366 55 341 8.03e-41 150
MS.gene02469.t1 AT1G17370 32.239 335 191 11 37 366 33 336 5.27e-40 147
MS.gene02469.t1 AT1G17370 32.537 335 187 12 37 366 33 333 1.13e-39 147
MS.gene02469.t1 AT4G34110 29.836 305 177 10 77 372 126 402 2.12e-25 108
MS.gene02469.t1 AT4G34110 26.071 280 163 8 76 350 37 277 2.99e-21 96.7
MS.gene02469.t1 AT2G23350 28.239 301 177 11 74 364 133 404 1.02e-24 107
MS.gene02469.t1 AT2G23350 25.874 286 160 10 74 350 45 287 3.84e-16 80.9
MS.gene02469.t1 AT2G23350 27.885 208 127 7 61 254 209 407 1.34e-14 76.3
MS.gene02469.t1 AT2G36660 25.000 288 170 7 76 358 25 271 9.70e-24 103
MS.gene02469.t1 AT2G36660 23.297 279 183 7 77 350 114 366 3.91e-14 74.7
MS.gene02469.t1 AT1G49760 26.246 301 193 7 77 372 135 411 1.88e-22 100
MS.gene02469.t1 AT1G49760 27.402 281 158 7 76 350 46 286 1.03e-19 92.0
MS.gene02469.t1 AT1G49760 26.246 301 193 7 77 372 135 411 1.88e-22 100
MS.gene02469.t1 AT1G49760 27.402 281 158 7 76 350 46 286 1.03e-19 92.0
MS.gene02469.t1 AT1G71770 25.763 295 188 9 77 364 148 418 4.34e-22 99.4
MS.gene02469.t1 AT1G71770 24.306 288 175 7 76 358 60 309 4.85e-19 89.7
MS.gene02469.t1 AT1G71770 25.763 295 188 9 77 364 148 418 4.34e-22 99.4
MS.gene02469.t1 AT1G71770 24.306 288 175 7 76 358 60 309 4.85e-19 89.7
MS.gene02469.t1 AT1G34140 25.676 296 185 8 77 365 33 300 9.52e-22 96.7
MS.gene02469.t1 AT1G34140 28.108 185 123 5 77 254 121 302 1.11e-12 69.7
MS.gene02469.t1 AT1G22760 24.731 372 182 12 76 363 50 407 4.31e-20 93.2
MS.gene02469.t1 AT1G22760 24.519 208 144 5 55 254 209 411 2.36e-12 69.3
MS.gene02469.t1 AT5G50250 32.778 180 115 3 71 250 110 283 1.13e-18 86.3
MS.gene02469.t1 AT4G24770 32.105 190 119 4 71 256 146 329 6.36e-18 84.7
MS.gene02469.t1 AT4G24770 32.105 190 119 4 71 256 146 329 6.36e-18 84.7
MS.gene02469.t1 AT2G18510 27.473 182 121 3 76 257 26 196 5.46e-17 82.4
MS.gene02469.t1 AT3G13224 28.125 192 128 5 75 265 10 192 1.01e-16 81.3
MS.gene02469.t1 AT3G16380 25.000 288 174 8 76 358 22 272 1.22e-16 82.4
MS.gene02469.t1 AT3G16380 25.430 291 179 10 77 358 114 375 3.36e-16 80.9
MS.gene02469.t1 AT3G52380 27.919 197 131 4 65 254 107 299 1.25e-16 80.9
MS.gene02469.t1 AT3G13224 28.042 189 126 5 77 264 21 200 2.37e-16 80.5
MS.gene02469.t1 AT4G26650 26.906 223 126 9 64 258 4 217 2.17e-14 75.1
MS.gene02469.t1 AT4G26650 26.108 203 124 7 163 353 4 192 5.80e-11 64.3
MS.gene02469.t1 AT4G26650 27.027 222 125 9 65 258 2 214 2.33e-14 75.1
MS.gene02469.t1 AT1G60000 29.730 185 122 5 71 254 80 257 2.60e-14 72.8
MS.gene02469.t1 AT3G04500 41.558 77 44 1 171 247 135 210 1.41e-13 70.5
MS.gene02469.t1 AT2G37220 27.500 200 122 4 77 256 93 289 1.05e-12 68.6
MS.gene02469.t1 AT2G37220 28.079 203 123 5 170 361 88 278 1.45e-11 65.1
MS.gene02469.t1 AT4G13850 40.741 81 47 1 175 255 37 116 1.96e-12 64.7
MS.gene02469.t1 AT2G33410 29.032 155 103 3 77 225 8 161 2.22e-12 68.6
MS.gene02469.t1 AT3G13224 28.395 162 107 4 77 238 21 173 2.59e-12 66.6
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 2.97e-12 68.6
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 2.97e-12 68.6
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 2.97e-12 68.6
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 2.97e-12 68.6
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 2.97e-12 68.6
MS.gene02469.t1 AT1G07360 37.255 102 60 2 285 385 220 318 3.55e-12 68.2
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 3.60e-12 68.2
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 3.60e-12 68.2
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 3.60e-12 68.2
MS.gene02469.t1 AT5G55550 26.633 199 116 7 73 254 4 189 3.60e-12 68.2
MS.gene02469.t1 AT4G13850 41.772 79 45 1 175 253 37 114 4.72e-12 63.9
MS.gene02469.t1 AT4G13850 41.772 79 45 1 175 253 37 114 5.74e-12 63.9
MS.gene02469.t1 AT3G07810 27.000 200 118 7 77 261 8 194 5.90e-12 67.8
MS.gene02469.t1 AT4G13850 41.772 79 45 1 175 253 37 114 6.38e-12 63.9
MS.gene02469.t1 AT3G07810 27.000 200 118 7 77 261 8 194 6.44e-12 67.4
MS.gene02469.t1 AT5G47620 26.630 184 121 5 77 253 8 184 1.89e-11 65.9
MS.gene02469.t1 AT5G47620 26.630 184 121 5 77 253 8 184 1.89e-11 65.9
MS.gene02469.t1 AT5G47620 26.630 184 121 5 77 253 8 184 1.89e-11 65.9
MS.gene02469.t1 AT2G29580 34.314 102 66 1 285 385 220 321 2.81e-11 65.5
MS.gene02469.t1 AT1G58470 28.492 179 114 7 175 346 8 179 6.54e-11 63.9
MS.gene02469.t1 AT1G58470 28.492 179 114 7 175 346 8 179 6.54e-11 63.9
MS.gene02469.t1 AT5G40490 25.414 181 118 4 77 254 44 210 7.32e-11 63.9

Find 113 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGCTGAGGAAGCAATTC+AGG 0.256444 4.2:+39903194 MS.gene02469:CDS
CGAAACGCCTGATCGGTATT+TGG 0.263962 4.2:-39901169 None:intergenic
ATCAGGCGTTTCGTCTGAAT+TGG 0.276433 4.2:+39901179 MS.gene02469:CDS
ACTGGTCAATCGGAGGGATA+TGG 0.328591 4.2:+39901079 MS.gene02469:CDS
GGATATGGTGGCCATGGATA+TGG 0.328731 4.2:+39903775 MS.gene02469:CDS
AGCAGACAGTGCGTCTTTCT+TGG 0.329108 4.2:+39903241 MS.gene02469:CDS
AAACTCACATGCTTATTTCC+TGG 0.336224 4.2:-39903273 None:intergenic
CATAACTGCTTTGCTCATAC+TGG 0.338799 4.2:+39900290 MS.gene02469:CDS
GCAGACAGTGCGTCTTTCTT+GGG 0.339350 4.2:+39903242 MS.gene02469:CDS
GGTTATGGAGGTCATGGATA+TGG 0.355121 4.2:+39903760 MS.gene02469:CDS
CTCTGTATTTGTGGGAGACT+TGG 0.355245 4.2:+39901264 MS.gene02469:CDS
CAACCCGCTGCTGCAGTTCA+AGG 0.361560 4.2:+39903841 MS.gene02469:CDS
CCCTTCAGATTGGGAACCTT+GGG 0.361796 4.2:-39902614 None:intergenic
AGGAATGACTCTAATGGTTA+TGG 0.376637 4.2:+39903745 MS.gene02469:CDS
CAGATCGTCCTGATCTCTTC+AGG 0.381114 4.2:-39900227 None:intergenic
ATCTGATCTCTCTGTATTTG+TGG 0.381754 4.2:+39901255 MS.gene02469:CDS
GGCTTGGTGACCTTCATCAT+TGG 0.396406 4.2:+39900249 MS.gene02469:CDS
TCAGGCGTTTCGTCTGAATT+GGG 0.404528 4.2:+39901180 MS.gene02469:CDS
TATCTGTTGCAGATATTTGT+TGG 0.405468 4.2:+39902721 MS.gene02469:intron
CATAGCATCAGTAACATCTA+TGG 0.413758 4.2:-39901287 None:intergenic
ATATGCTGCTAGACAAAATC+AGG 0.422308 4.2:+39903807 MS.gene02469:CDS
CCCCTTCAGATTGGGAACCT+TGG 0.423023 4.2:-39902615 None:intergenic
GGATTGAATGGAACAGTGAT+TGG 0.427017 4.2:+39903216 MS.gene02469:CDS
ACCTTGGGCCACAGCACCAT+TGG 0.430193 4.2:-39902599 None:intergenic
ACATTCAGCGCTGCCGGTGG+TGG 0.434038 4.2:+39901205 MS.gene02469:CDS
AGCTGGCGCCTGAAGAGATC+AGG 0.435334 4.2:+39900219 MS.gene02469:CDS
CAAGTGTCGCCGCCTCCGAC+GGG 0.435710 4.2:+39900140 MS.gene02469:CDS
GTCATACGTAATAAGCAAAC+TGG 0.435726 4.2:+39901061 MS.gene02469:CDS
GTTAGAATCCCCTTCAGATT+GGG 0.438640 4.2:-39902623 None:intergenic
GCAGCATCAGCAGAAGCAGC+TGG 0.446100 4.2:+39900202 MS.gene02469:CDS
CTTTCTTGGGGTCGCAGTCC+AGG 0.447144 4.2:+39903255 MS.gene02469:CDS
GGCTTATGTGCAGGTTGTAT+CGG 0.450307 4.2:+39901033 MS.gene02469:intron
GTGAAAATCCCAGTCGGTAA+AGG 0.454342 4.2:+39902814 MS.gene02469:CDS
TTCAGCGCTGCCGGTGGTGG+TGG 0.454357 4.2:+39901208 MS.gene02469:CDS
TCTAATACTGGTCGTTCAAA+AGG 0.454872 4.2:+39901370 MS.gene02469:CDS
GTTGTTATTGACTCTAATAC+TGG 0.457060 4.2:+39901358 MS.gene02469:CDS
CTGAAGAGATCAGGACGATC+TGG 0.457665 4.2:+39900228 MS.gene02469:CDS
TCTGATCTCTCTGTATTTGT+GGG 0.467526 4.2:+39901256 MS.gene02469:CDS
TGTTAGAATCCCCTTCAGAT+TGG 0.468873 4.2:-39902624 None:intergenic
GCCCCTTGAACTGCAGCAGC+GGG 0.471724 4.2:-39903844 None:intergenic
AACCCGCTGCTGCAGTTCAA+GGG 0.482024 4.2:+39903842 MS.gene02469:CDS
GAAGCAATTCAGGGATTGAA+TGG 0.482531 4.2:+39903204 MS.gene02469:CDS
ACGCCTCTCGCCACCACCAC+CGG 0.482553 4.2:-39901218 None:intergenic
ATGGCGTTTATTGTTCTAGC+AGG 0.496066 4.2:+39901452 MS.gene02469:CDS
TAATAAGCAAACTGGTCAAT+CGG 0.501480 4.2:+39901069 MS.gene02469:CDS
AAAGTCTTGCAGAACTTCAA+TGG 0.502491 4.2:+39901136 MS.gene02469:CDS
GTAGTGTTGGCTGGTGGCGG+TGG 0.503654 4.2:+39902571 MS.gene02469:CDS
TCTTAAACTAGCTGTAGTGT+TGG 0.504172 4.2:+39902558 MS.gene02469:intron
AAACTAGCTGTAGTGTTGGC+TGG 0.505517 4.2:+39902562 MS.gene02469:intron
TCTAATGGTTATGGAGGTCA+TGG 0.506688 4.2:+39903754 MS.gene02469:CDS
GCTGATATTGATGCTGCTGA+TGG 0.508419 4.2:-39900181 None:intergenic
TGGGCTACATTCAGCGCTGC+CGG 0.511139 4.2:+39901199 MS.gene02469:CDS
TAATGATGAGGATGAACATA+AGG 0.511533 4.2:-39900092 None:intergenic
GCAAGTGTCGCCGCCTCCGA+CGG 0.514870 4.2:+39900139 MS.gene02469:CDS
GAGATCAGGACGATCTGGCT+TGG 0.515806 4.2:+39900233 MS.gene02469:CDS
TCCAATGGTGCTGTGGCCCA+AGG 0.516661 4.2:+39902598 MS.gene02469:CDS
AAACCACATCCTTTACCGAC+TGG 0.519643 4.2:-39902823 None:intergenic
TATGATGCCAAATACCGATC+AGG 0.523250 4.2:+39901162 MS.gene02469:CDS
CTGCCGGTGGTGGTGGCGAG+AGG 0.523668 4.2:+39901215 MS.gene02469:CDS
AGCCCCTTGAACTGCAGCAG+CGG 0.525089 4.2:-39903845 None:intergenic
ACTGGTCGTTCAAAAGGCTA+TGG 0.525221 4.2:+39901376 MS.gene02469:CDS
AACCACATCCTTTACCGACT+GGG 0.528514 4.2:-39902822 None:intergenic
TGGTGACCTTCATCATTGGA+TGG 0.528713 4.2:+39900253 MS.gene02469:CDS
TTGCTTATTACGTATGACCT+TGG 0.529532 4.2:-39901056 None:intergenic
CTGTTGCAGATATTTGTTGG+AGG 0.534128 4.2:+39902724 MS.gene02469:intron
ACCCGCTGCTGCAGTTCAAG+GGG 0.535252 4.2:+39903843 MS.gene02469:CDS
GGTCATGGATATGGTGGCCA+TGG 0.539115 4.2:+39903769 MS.gene02469:CDS
ATCCATTACTTCCATATCCA+TGG 0.539208 4.2:-39903786 None:intergenic
CAGTGGAGGAATGACTCTAA+TGG 0.541519 4.2:+39903739 MS.gene02469:intron
TGACTATGCCCCGTCGGAGG+CGG 0.541638 4.2:-39900149 None:intergenic
CCGACGGGGCATAGTCATCA+TGG 0.543230 4.2:+39900155 MS.gene02469:CDS
TCTCATCCATCCAATGATGA+AGG 0.546643 4.2:-39900259 None:intergenic
ATCCCAGTCGGTAAAGGATG+TGG 0.548381 4.2:+39902820 MS.gene02469:CDS
CCTTGCGAAGAATATTGTTG+TGG 0.552246 4.2:-39901520 None:intergenic
GGCCATGGATATGGAAGTAA+TGG 0.553720 4.2:+39903784 MS.gene02469:CDS
GCTGTAGTGTTGGCTGGTGG+CGG 0.557579 4.2:+39902568 MS.gene02469:intron
GCCCAAGGTTCCCAATCTGA+AGG 0.557760 4.2:+39902613 MS.gene02469:CDS
CCACAACAATATTCTTCGCA+AGG 0.559021 4.2:+39901520 MS.gene02469:CDS
AATGCTGAGGAAGCAATTCA+GGG 0.562076 4.2:+39903195 MS.gene02469:CDS
ATTCAGACGAAACGCCTGAT+CGG 0.563201 4.2:-39901176 None:intergenic
ATCATAGCCATAGCAGTAGC+TGG 0.566838 4.2:-39900032 None:intergenic
CTGCTTTGCTCATACTGGAG+AGG 0.568397 4.2:+39900295 MS.gene02469:CDS
CAGTTCAAGGGGCTTCCTAA+CGG 0.574226 4.2:+39903854 MS.gene02469:CDS
TGGCGAGAGGCGTTCTTCTG+AGG 0.575371 4.2:+39901228 MS.gene02469:CDS
GGTGGCGGTGGACACTCCAA+TGG 0.577143 4.2:+39902583 MS.gene02469:CDS
CGAGTTCTATACGCGAGCAA+TGG 0.581423 4.2:+39901108 MS.gene02469:CDS
AGAGCAATGACTGAAATGAA+TGG 0.587516 4.2:+39901433 MS.gene02469:CDS
GACACTTGCTGTTGATGAGG+TGG 0.591335 4.2:-39900125 None:intergenic
CCCAAGGTTCCCAATCTGAA+GGG 0.591408 4.2:+39902614 MS.gene02469:CDS
GCAGTATCCAGCTACTGCTA+TGG 0.591918 4.2:+39900025 MS.gene02469:CDS
AAGCAAACTGGTCAATCGGA+GGG 0.598971 4.2:+39901073 MS.gene02469:CDS
AAGTGTCGCCGCCTCCGACG+GGG 0.600014 4.2:+39900141 MS.gene02469:CDS
CAGACAGTGCGTCTTTCTTG+GGG 0.601300 4.2:+39903243 MS.gene02469:CDS
TGGACACTCCAATGGTGCTG+TGG 0.602089 4.2:+39902591 MS.gene02469:CDS
CCATGATGACTATGCCCCGT+CGG 0.605517 4.2:-39900155 None:intergenic
TGTGCAGGTTGTATCGGCCA+AGG 0.610498 4.2:+39901039 MS.gene02469:intron
GGCGACACTTGCTGTTGATG+AGG 0.611155 4.2:-39900128 None:intergenic
GGTTTGGTGATGAAAGTGAA+AGG 0.613030 4.2:+39901407 MS.gene02469:CDS
TAAGCAAACTGGTCAATCGG+AGG 0.614770 4.2:+39901072 MS.gene02469:CDS
ATCTCTGTGAAAATCCCAGT+CGG 0.624783 4.2:+39902808 MS.gene02469:CDS
AATGACTCTAATGGTTATGG+AGG 0.625005 4.2:+39903748 MS.gene02469:CDS
GCTACATTCAGCGCTGCCGG+TGG 0.628050 4.2:+39901202 MS.gene02469:CDS
TCTAGCAGGCCTATGCGAGT+AGG 0.628642 4.2:+39901466 MS.gene02469:CDS
TGACTCTGACATTAGTGATG+AGG 0.629614 4.2:+39902750 MS.gene02469:CDS
TATGGAGGTCATGGATATGG+TGG 0.629902 4.2:+39903763 MS.gene02469:CDS
CCAAGGTTCCCAATCTGAAG+GGG 0.630101 4.2:+39902615 MS.gene02469:CDS
TGATGACTATGCCCCGTCGG+AGG 0.632739 4.2:-39900152 None:intergenic
GTTGCAACACCTACTCGCAT+AGG 0.642755 4.2:-39901475 None:intergenic
GGCTGCTGCTGATAATGATG+AGG 0.650096 4.2:-39900104 None:intergenic
ATGTTACAGAAAGAATGCTG+AGG 0.670854 4.2:+39903182 MS.gene02469:intron
ACATAAGGCATATAATGCTG+AGG 0.677746 4.2:-39900077 None:intergenic
CTAGCTGTAGTGTTGGCTGG+TGG 0.692590 4.2:+39902565 MS.gene02469:intron
ACGGGGCATAGTCATCATGG+AGG 0.747794 4.2:+39900158 MS.gene02469:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTTAATAAACATCTA+TGG - chr4.2:39901989-39902008 None:intergenic 10.0%
!!! GGAAATTTTATAAAATAATT+TGG - chr4.2:39903515-39903534 None:intergenic 10.0%
!! AAAGTATAACTATCTTATAT+GGG - chr4.2:39900732-39900751 None:intergenic 15.0%
!!! AAAAGTATAACTATCTTATA+TGG - chr4.2:39900733-39900752 None:intergenic 15.0%
!!! AAATTTTCATTATAACTGAA+AGG + chr4.2:39901602-39901621 MS.gene02469:intron 15.0%
!!! AGTTTCTGTTTTTATATAAA+TGG + chr4.2:39900418-39900437 MS.gene02469:intron 15.0%
!!! ATATAAGATAGTTATACTTT+TGG + chr4.2:39900732-39900751 MS.gene02469:intron 15.0%
!! AAAAATAAGACGATACATAT+TGG - chr4.2:39903118-39903137 None:intergenic 20.0%
!! AACATTCAATAAATAGATTG+AGG - chr4.2:39902325-39902344 None:intergenic 20.0%
!! AATATCCTACAATACATTAT+TGG - chr4.2:39900897-39900916 None:intergenic 20.0%
!! ACAAAGAATTAAATTAGAGA+TGG - chr4.2:39903712-39903731 None:intergenic 20.0%
!! ACGACAATAAAAAAAAAGTT+TGG - chr4.2:39900982-39901001 None:intergenic 20.0%
!! ATGATATAAGATTACTTGAT+TGG - chr4.2:39903680-39903699 None:intergenic 20.0%
!! GTAAATATCATATTTGTTTC+TGG + chr4.2:39901861-39901880 MS.gene02469:intron 20.0%
!!! AAAAGTGAGAATTCATTTTA+GGG - chr4.2:39903643-39903662 None:intergenic 20.0%
!!! AGCAAATTTTCATCTATTAA+GGG + chr4.2:39901328-39901347 MS.gene02469:CDS 20.0%
!!! GGCAGAAAAATTAATTTTTA+TGG + chr4.2:39902413-39902432 MS.gene02469:intron 20.0%
!!! TAAAAATTAATTTTTCTGCC+TGG - chr4.2:39902413-39902432 None:intergenic 20.0%
!!! TAAAAGTGAGAATTCATTTT+AGG - chr4.2:39903644-39903663 None:intergenic 20.0%
!!! TAGCAAATTTTCATCTATTA+AGG + chr4.2:39901327-39901346 MS.gene02469:CDS 20.0%
!!! TTAATTGCACTTTGAAAAAT+GGG + chr4.2:39900848-39900867 MS.gene02469:intron 20.0%
!!! TTGTTGTTATATTTTCACTT+TGG + chr4.2:39903318-39903337 MS.gene02469:intron 20.0%
!!! TTTGATTTGAGATTGATTTA+AGG + chr4.2:39900515-39900534 MS.gene02469:intron 20.0%
! ATATTAATATCAATCCAACC+AGG - chr4.2:39901648-39901667 None:intergenic 25.0%
! ATTGGACTCTTTAATGAATT+AGG + chr4.2:39900650-39900669 MS.gene02469:intron 25.0%
! ATTTATTGCTGGAAGTTATT+AGG + chr4.2:39900446-39900465 MS.gene02469:intron 25.0%
! CTCAGTGATTAATTAAATCA+AGG - chr4.2:39900543-39900562 None:intergenic 25.0%
! CTTGATTTAATTAATCACTG+AGG + chr4.2:39900541-39900560 MS.gene02469:intron 25.0%
! GACTTCATAATCAAAATCTA+AGG - chr4.2:39903621-39903640 None:intergenic 25.0%
! TATGCATGAATATTTGTTTC+TGG + chr4.2:39901958-39901977 MS.gene02469:intron 25.0%
! TATTAATATCAATCCAACCA+GGG - chr4.2:39901647-39901666 None:intergenic 25.0%
! TTAAATCTCATATTTGTTCC+TGG + chr4.2:39902128-39902147 MS.gene02469:intron 25.0%
! TTGGACTCTTTAATGAATTA+GGG + chr4.2:39900651-39900670 MS.gene02469:intron 25.0%
! TTTATTGCTGGAAGTTATTA+GGG + chr4.2:39900447-39900466 MS.gene02469:intron 25.0%
! TTTGTCCAATAATGTATTGT+AGG + chr4.2:39900889-39900908 MS.gene02469:intron 25.0%
!! ATCTTTTTGTGTGTGATTAT+TGG + chr4.2:39900632-39900651 MS.gene02469:intron 25.0%
!! CTTAATTGCACTTTGAAAAA+TGG + chr4.2:39900847-39900866 MS.gene02469:intron 25.0%
!! TAATTGCACTTTGAAAAATG+GGG + chr4.2:39900849-39900868 MS.gene02469:intron 25.0%
!! TTCTTTTGATATCTCAGTAA+CGG + chr4.2:39902697-39902716 MS.gene02469:intron 25.0%
!! TTTTCATTATAACTGAAAGG+AGG + chr4.2:39901605-39901624 MS.gene02469:intron 25.0%
!!! GTGCATATCTATTTTTATGT+TGG + chr4.2:39901697-39901716 MS.gene02469:intron 25.0%
AAAGTGCAATTAAGAGATCT+AGG - chr4.2:39900841-39900860 None:intergenic 30.0%
AACAGTATACATCATGAGAT+AGG - chr4.2:39902270-39902289 None:intergenic 30.0%
AACCAGGGAATTGAATTATT+AGG - chr4.2:39901632-39901651 None:intergenic 30.0%
AAGTATTCCTCAAATGGATA+GGG + chr4.2:39902971-39902990 MS.gene02469:intron 30.0%
ACATCACCAAATTGCAAAAA+TGG - chr4.2:39902787-39902806 None:intergenic 30.0%
ACGATCTATATCTATGCTAA+GGG - chr4.2:39902882-39902901 None:intergenic 30.0%
AGCACACTTACAAATAAAAC+AGG - chr4.2:39901767-39901786 None:intergenic 30.0%
GCAAGTCTAGTTAAAAACAT+TGG - chr4.2:39900810-39900829 None:intergenic 30.0%
GTATGATGAATAAATCGAAG+TGG + chr4.2:39901741-39901760 MS.gene02469:intron 30.0%
GTTAAAAACATTGGTCAACA+AGG - chr4.2:39900801-39900820 None:intergenic 30.0%
GTTATGCATGAATTCAAACA+TGG + chr4.2:39901932-39901951 MS.gene02469:intron 30.0%
TAAGTATTCCTCAAATGGAT+AGG + chr4.2:39902970-39902989 MS.gene02469:intron 30.0%
TAATAAGCAAACTGGTCAAT+CGG + chr4.2:39901069-39901088 MS.gene02469:CDS 30.0%
TAATAATTCAATTCCCTGGT+TGG + chr4.2:39901631-39901650 MS.gene02469:intron 30.0%
TAATGATGAGGATGAACATA+AGG - chr4.2:39900095-39900114 None:intergenic 30.0%
TACGATCTATATCTATGCTA+AGG - chr4.2:39902883-39902902 None:intergenic 30.0%
TATCTGTTGCAGATATTTGT+TGG + chr4.2:39902721-39902740 MS.gene02469:intron 30.0%
TGCAAGGAAATATAACAAAG+TGG - chr4.2:39902226-39902245 None:intergenic 30.0%
TTAGTACACAAACTTAGAAC+AGG - chr4.2:39903390-39903409 None:intergenic 30.0%
TTGACTCTTGTGATTTAAGT+TGG + chr4.2:39900681-39900700 MS.gene02469:intron 30.0%
TTGGTCATAAATGCGATAAT+CGG - chr4.2:39900963-39900982 None:intergenic 30.0%
TTTAGTAGTGTAAGCAATAC+AGG + chr4.2:39903429-39903448 MS.gene02469:intron 30.0%
! AAATGGTCTTGATTTATTGC+TGG + chr4.2:39900435-39900454 MS.gene02469:intron 30.0%
! TGACTTTTCAATACAAGTCT+TGG + chr4.2:39901809-39901828 MS.gene02469:intron 30.0%
! TTTTATTGTCGTCTTCTGAT+TGG + chr4.2:39900990-39901009 MS.gene02469:intron 30.0%
!! AGACAACCATTTTTGCAATT+TGG + chr4.2:39902778-39902797 MS.gene02469:CDS 30.0%
!! GTTGTTATTGACTCTAATAC+TGG + chr4.2:39901358-39901377 MS.gene02469:CDS 30.0%
!!! GGATTGCCATAAGTTTTTTT+GGG - chr4.2:39901502-39901521 None:intergenic 30.0%
!!! GTATCGTCTTATTTTTGACT+GGG + chr4.2:39903122-39903141 MS.gene02469:intron 30.0%
!!! GTTTGTTGATGTTTTTTCGT+TGG + chr4.2:39901012-39901031 MS.gene02469:intron 30.0%
!!! TGGATTGCCATAAGTTTTTT+TGG - chr4.2:39901503-39901522 None:intergenic 30.0%
!!! TGTATCGTCTTATTTTTGAC+TGG + chr4.2:39903121-39903140 MS.gene02469:intron 30.0%
AAACTCACATGCTTATTTCC+TGG - chr4.2:39903276-39903295 None:intergenic 35.0%
AAAGTCTTGCAGAACTTCAA+TGG + chr4.2:39901136-39901155 MS.gene02469:CDS 35.0%
AATGACTCTAATGGTTATGG+AGG + chr4.2:39903748-39903767 MS.gene02469:CDS 35.0%
ACATAAGGCATATAATGCTG+AGG - chr4.2:39900080-39900099 None:intergenic 35.0%
AGAATCTTGAATGCATAACC+AGG + chr4.2:39902392-39902411 MS.gene02469:intron 35.0%
AGAGCAATGACTGAAATGAA+TGG + chr4.2:39901433-39901452 MS.gene02469:CDS 35.0%
AGCGCACTTACAAATAAAAC+AGG - chr4.2:39902037-39902056 None:intergenic 35.0%
AGGAATGACTCTAATGGTTA+TGG + chr4.2:39903745-39903764 MS.gene02469:CDS 35.0%
ATACATCATGAGATAGGCAA+GGG - chr4.2:39902264-39902283 None:intergenic 35.0%
ATATGCTGCTAGACAAAATC+AGG + chr4.2:39903807-39903826 MS.gene02469:CDS 35.0%
ATCCATTACTTCCATATCCA+TGG - chr4.2:39903789-39903808 None:intergenic 35.0%
ATCTGATCTCTCTGTATTTG+TGG + chr4.2:39901255-39901274 MS.gene02469:CDS 35.0%
ATGAAGAGTTGGCATAATCA+AGG - chr4.2:39903000-39903019 None:intergenic 35.0%
ATGTTACAGAAAGAATGCTG+AGG + chr4.2:39903182-39903201 MS.gene02469:intron 35.0%
CATAGCATCAGTAACATCTA+TGG - chr4.2:39901290-39901309 None:intergenic 35.0%
GCAACACCCAAAAAAACTTA+TGG + chr4.2:39901493-39901512 MS.gene02469:CDS 35.0%
GTCATACGTAATAAGCAAAC+TGG + chr4.2:39901061-39901080 MS.gene02469:CDS 35.0%
GTCCTAATAATTCAATTCCC+TGG + chr4.2:39901627-39901646 MS.gene02469:intron 35.0%
TATACATCATGAGATAGGCA+AGG - chr4.2:39902265-39902284 None:intergenic 35.0%
TCTAATACTGGTCGTTCAAA+AGG + chr4.2:39901370-39901389 MS.gene02469:CDS 35.0%
TCTGATCTCTCTGTATTTGT+GGG + chr4.2:39901256-39901275 MS.gene02469:CDS 35.0%
TCTGTAACATAAAGAGCAGA+AGG - chr4.2:39903172-39903191 None:intergenic 35.0%
TCTTAAACTAGCTGTAGTGT+TGG + chr4.2:39902558-39902577 MS.gene02469:intron 35.0%
TGGCATAAGTATTCCTCAAA+TGG + chr4.2:39902965-39902984 MS.gene02469:intron 35.0%
TTGCTTATTACGTATGACCT+TGG - chr4.2:39901059-39901078 None:intergenic 35.0%
TTTCACTTTGGCCATCTAAT+GGG + chr4.2:39903330-39903349 MS.gene02469:intron 35.0%
! ATGCTGAAGGTATATGAATG+CGG - chr4.2:39903536-39903555 None:intergenic 35.0%
! CTCGTAATTTTCCCATTAGA+TGG - chr4.2:39903344-39903363 None:intergenic 35.0%
! TATACTTTTGGTACCCTAAG+TGG + chr4.2:39900744-39900763 MS.gene02469:intron 35.0%
! TGGTTTCCTTTTTCTATCCT+TGG + chr4.2:39900701-39900720 MS.gene02469:intron 35.0%
! TTTTCACTTTGGCCATCTAA+TGG + chr4.2:39903329-39903348 MS.gene02469:intron 35.0%
!! TTCCTGGCTTTATGCATTAA+TGG + chr4.2:39902144-39902163 MS.gene02469:intron 35.0%
!!! CAAAAGGCTATGGTTTTGTT+AGG + chr4.2:39901386-39901405 MS.gene02469:CDS 35.0%
AAGGATTCCCTATCCATTTG+AGG - chr4.2:39902981-39903000 None:intergenic 40.0%
AATGCTGAGGAAGCAATTCA+GGG + chr4.2:39903195-39903214 MS.gene02469:CDS 40.0%
AATTCTTTGTGATGTCGCAG+TGG + chr4.2:39903722-39903741 MS.gene02469:intron 40.0%
AGCAACTAGTTACATGCTGA+AGG - chr4.2:39903549-39903568 None:intergenic 40.0%
ATCTCTGTGAAAATCCCAGT+CGG + chr4.2:39902808-39902827 MS.gene02469:CDS 40.0%
ATCTTATATGGGCAATGCCA+AGG - chr4.2:39900721-39900740 None:intergenic 40.0%
ATGGCGTTTATTGTTCTAGC+AGG + chr4.2:39901452-39901471 MS.gene02469:CDS 40.0%
CAATGTCTAAGCGTCAAAGT+CGG - chr4.2:39900940-39900959 None:intergenic 40.0%
CATAACTGCTTTGCTCATAC+TGG + chr4.2:39900290-39900309 MS.gene02469:CDS 40.0%
CCACAACAATATTCTTCGCA+AGG + chr4.2:39901520-39901539 MS.gene02469:CDS 40.0%
CCTTGCGAAGAATATTGTTG+TGG - chr4.2:39901523-39901542 None:intergenic 40.0%
GAAGCAATTCAGGGATTGAA+TGG + chr4.2:39903204-39903223 MS.gene02469:CDS 40.0%
GAATAGATTCAAGTGGTGCA+AGG - chr4.2:39902242-39902261 None:intergenic 40.0%
GCAATGCCAAGGATAGAAAA+AGG - chr4.2:39900710-39900729 None:intergenic 40.0%
GGATTGAATGGAACAGTGAT+TGG + chr4.2:39903216-39903235 MS.gene02469:CDS 40.0%
GTCCATTAATGCATAAAGCC+AGG - chr4.2:39902149-39902168 None:intergenic 40.0%
GTTAGAATCCCCTTCAGATT+GGG - chr4.2:39902626-39902645 None:intergenic 40.0%
GTTTCTTGCGAAATGAACAG+TGG + chr4.2:39902945-39902964 MS.gene02469:intron 40.0%
TAACACGAGAGAGTTATCAC+TGG + chr4.2:39902476-39902495 MS.gene02469:intron 40.0%
TATGATGCCAAATACCGATC+AGG + chr4.2:39901162-39901181 MS.gene02469:CDS 40.0%
TCATCCTGTGGAATCATGTA+AGG + chr4.2:39903016-39903035 MS.gene02469:intron 40.0%
TCATCCTTACATGATTCCAC+AGG - chr4.2:39903023-39903042 None:intergenic 40.0%
TCTAATGGTTATGGAGGTCA+TGG + chr4.2:39903754-39903773 MS.gene02469:CDS 40.0%
TCTCATCCATCCAATGATGA+AGG - chr4.2:39900262-39900281 None:intergenic 40.0%
TGACTCTGACATTAGTGATG+AGG + chr4.2:39902750-39902769 MS.gene02469:CDS 40.0%
TGTGGAATCATGTAAGGATG+AGG + chr4.2:39903022-39903041 MS.gene02469:intron 40.0%
TGTTAGAATCCCCTTCAGAT+TGG - chr4.2:39902627-39902646 None:intergenic 40.0%
TTTCGGCCTTCAAAACTCTA+GGG - chr4.2:39900772-39900791 None:intergenic 40.0%
! CTGTTGCAGATATTTGTTGG+AGG + chr4.2:39902724-39902743 MS.gene02469:intron 40.0%
! GGTCAACAAGGTCACATTTT+CGG - chr4.2:39900789-39900808 None:intergenic 40.0%
! TGAAGTATCTGCAGCAGAAA+AGG - chr4.2:39901583-39901602 None:intergenic 40.0%
! TTTTCGGCCTTCAAAACTCT+AGG - chr4.2:39900773-39900792 None:intergenic 40.0%
!! AAGGTTGGTGCTGATTTAAG+TGG - chr4.2:39901564-39901583 None:intergenic 40.0%
!! GGTTTGGTGATGAAAGTGAA+AGG + chr4.2:39901407-39901426 MS.gene02469:CDS 40.0%
!!! GGCTATGGTTTTGTTAGGTT+TGG + chr4.2:39901391-39901410 MS.gene02469:CDS 40.0%
!!! GTTTTGTTCAACTTGCTGAC+AGG + chr4.2:39902842-39902861 MS.gene02469:CDS 40.0%
!!! TCGTCTTATTTTTGACTGGG+TGG + chr4.2:39903125-39903144 MS.gene02469:intron 40.0%
!!! TTTTTCGTTGGCTTATGTGC+AGG + chr4.2:39901024-39901043 MS.gene02469:intron 40.0%
AAACCACATCCTTTACCGAC+TGG - chr4.2:39902826-39902845 None:intergenic 45.0%
AAGCAAACTGGTCAATCGGA+GGG + chr4.2:39901073-39901092 MS.gene02469:CDS 45.0%
ACACAACTGTGAGTCTGCTT+TGG + chr4.2:39902647-39902666 MS.gene02469:intron 45.0%
ACTGGTCGTTCAAAAGGCTA+TGG + chr4.2:39901376-39901395 MS.gene02469:CDS 45.0%
ATCAGGCGTTTCGTCTGAAT+TGG + chr4.2:39901179-39901198 MS.gene02469:CDS 45.0%
ATCATAGCCATAGCAGTAGC+TGG - chr4.2:39900035-39900054 None:intergenic 45.0%
ATTCAGACGAAACGCCTGAT+CGG - chr4.2:39901179-39901198 None:intergenic 45.0%
CAGTGGAGGAATGACTCTAA+TGG + chr4.2:39903739-39903758 MS.gene02469:intron 45.0%
CTCTGTATTTGTGGGAGACT+TGG + chr4.2:39901264-39901283 MS.gene02469:CDS 45.0%
GAATGCTGAGGAAGCAATTC+AGG + chr4.2:39903194-39903213 MS.gene02469:CDS 45.0%
GATTCCACAGGATGAAGAGT+TGG - chr4.2:39903011-39903030 None:intergenic 45.0%
GCTGATATTGATGCTGCTGA+TGG - chr4.2:39900184-39900203 None:intergenic 45.0%
GCTGGAAGTTATTAGGGTAC+AGG + chr4.2:39900453-39900472 MS.gene02469:intron 45.0%
GGCAAGGGAATAGATTCAAG+TGG - chr4.2:39902249-39902268 None:intergenic 45.0%
GGCCATGGATATGGAAGTAA+TGG + chr4.2:39903784-39903803 MS.gene02469:CDS 45.0%
GGCTTATGTGCAGGTTGTAT+CGG + chr4.2:39901033-39901052 MS.gene02469:intron 45.0%
GGTTATGGAGGTCATGGATA+TGG + chr4.2:39903760-39903779 MS.gene02469:CDS 45.0%
GTATCTGCAGCAGAAAAGGT+TGG - chr4.2:39901579-39901598 None:intergenic 45.0%
GTCTCGTTCTCTCTGTAATC+AGG + chr4.2:39903363-39903382 MS.gene02469:intron 45.0%
GTGAAAATCCCAGTCGGTAA+AGG + chr4.2:39902814-39902833 MS.gene02469:CDS 45.0%
TAAGCAAACTGGTCAATCGG+AGG + chr4.2:39901072-39901091 MS.gene02469:CDS 45.0%
TATGCCAACTCTTCATCCTG+TGG + chr4.2:39903004-39903023 MS.gene02469:intron 45.0%
TATGGAGGTCATGGATATGG+TGG + chr4.2:39903763-39903782 MS.gene02469:CDS 45.0%
TCAGGCGTTTCGTCTGAATT+GGG + chr4.2:39901180-39901199 MS.gene02469:CDS 45.0%
TGGTGACCTTCATCATTGGA+TGG + chr4.2:39900253-39900272 MS.gene02469:CDS 45.0%
TTCGGCCTTCAAAACTCTAG+GGG - chr4.2:39900771-39900790 None:intergenic 45.0%
! AAAACTCTAGGGGACCACTT+AGG - chr4.2:39900761-39900780 None:intergenic 45.0%
! AAACTCTAGGGGACCACTTA+GGG - chr4.2:39900760-39900779 None:intergenic 45.0%
! AACCACATCCTTTACCGACT+GGG - chr4.2:39902825-39902844 None:intergenic 45.0%
!! AAACTAGCTGTAGTGTTGGC+TGG + chr4.2:39902562-39902581 MS.gene02469:intron 45.0%
ACTGGTCAATCGGAGGGATA+TGG + chr4.2:39901079-39901098 MS.gene02469:CDS 50.0%
AGCAGACAGTGCGTCTTTCT+TGG + chr4.2:39903241-39903260 MS.gene02469:CDS 50.0%
ATCCCAGTCGGTAAAGGATG+TGG + chr4.2:39902820-39902839 MS.gene02469:CDS 50.0%
CAGACAGTGCGTCTTTCTTG+GGG + chr4.2:39903243-39903262 MS.gene02469:CDS 50.0%
CAGATCGTCCTGATCTCTTC+AGG - chr4.2:39900230-39900249 None:intergenic 50.0%
CCAAGGTTCCCAATCTGAAG+GGG + chr4.2:39902615-39902634 MS.gene02469:CDS 50.0%
CCCAAGGTTCCCAATCTGAA+GGG + chr4.2:39902614-39902633 MS.gene02469:CDS 50.0%
CCCTTCAGATTGGGAACCTT+GGG - chr4.2:39902617-39902636 None:intergenic 50.0%
CGAAACGCCTGATCGGTATT+TGG - chr4.2:39901172-39901191 None:intergenic 50.0%
CGAGTTCTATACGCGAGCAA+TGG + chr4.2:39901108-39901127 MS.gene02469:CDS 50.0%
CTGAAGAGATCAGGACGATC+TGG + chr4.2:39900228-39900247 MS.gene02469:CDS 50.0%
CTGCTTTGCTCATACTGGAG+AGG + chr4.2:39900295-39900314 MS.gene02469:CDS 50.0%
CTTGCTGACAGGTACACTTG+TGG + chr4.2:39902853-39902872 MS.gene02469:intron 50.0%
GCAGACAGTGCGTCTTTCTT+GGG + chr4.2:39903242-39903261 MS.gene02469:CDS 50.0%
GCAGTATCCAGCTACTGCTA+TGG + chr4.2:39900025-39900044 MS.gene02469:CDS 50.0%
GGATATGGTGGCCATGGATA+TGG + chr4.2:39903775-39903794 MS.gene02469:CDS 50.0%
GTTGCAACACCTACTCGCAT+AGG - chr4.2:39901478-39901497 None:intergenic 50.0%
TCTTTGTGATGTCGCAGTGG+AGG + chr4.2:39903725-39903744 MS.gene02469:intron 50.0%
! GGCTGCTGCTGATAATGATG+AGG - chr4.2:39900107-39900126 None:intergenic 50.0%
!! GACACTTGCTGTTGATGAGG+TGG - chr4.2:39900128-39900147 None:intergenic 50.0%
!! GGCTTGGTGACCTTCATCAT+TGG + chr4.2:39900249-39900268 MS.gene02469:CDS 50.0%
!! GTGGTCCCCTAGAGTTTTGA+AGG + chr4.2:39900763-39900782 MS.gene02469:intron 50.0%
AACCCGCTGCTGCAGTTCAA+GGG + chr4.2:39903842-39903861 MS.gene02469:CDS 55.0%
ACGGGGCATAGTCATCATGG+AGG + chr4.2:39900158-39900177 MS.gene02469:CDS 55.0%
CCATGATGACTATGCCCCGT+CGG - chr4.2:39900158-39900177 None:intergenic 55.0%
CCCCTTCAGATTGGGAACCT+TGG - chr4.2:39902618-39902637 None:intergenic 55.0%
GAGATCAGGACGATCTGGCT+TGG + chr4.2:39900233-39900252 MS.gene02469:CDS 55.0%
GCCCAAGGTTCCCAATCTGA+AGG + chr4.2:39902613-39902632 MS.gene02469:CDS 55.0%
GGTCATGGATATGGTGGCCA+TGG + chr4.2:39903769-39903788 MS.gene02469:CDS 55.0%
TCTAGCAGGCCTATGCGAGT+AGG + chr4.2:39901466-39901485 MS.gene02469:CDS 55.0%
! TGGACACTCCAATGGTGCTG+TGG + chr4.2:39902591-39902610 MS.gene02469:CDS 55.0%
! TGTGCAGGTTGTATCGGCCA+AGG + chr4.2:39901039-39901058 MS.gene02469:intron 55.0%
!! CTAGCTGTAGTGTTGGCTGG+TGG + chr4.2:39902565-39902584 MS.gene02469:intron 55.0%
!! GGCGACACTTGCTGTTGATG+AGG - chr4.2:39900131-39900150 None:intergenic 55.0%
AAACCGCCCACTGTGCTGTG+GGG - chr4.2:39902929-39902948 None:intergenic 60.0%
ACCCGCTGCTGCAGTTCAAG+GGG + chr4.2:39903843-39903862 MS.gene02469:CDS 60.0%
ACCTTGGGCCACAGCACCAT+TGG - chr4.2:39902602-39902621 None:intergenic 60.0%
AGAAACCGCCCACTGTGCTG+TGG - chr4.2:39902931-39902950 None:intergenic 60.0%
AGCCCCTTGAACTGCAGCAG+CGG - chr4.2:39903848-39903867 None:intergenic 60.0%
AGCTGGCGCCTGAAGAGATC+AGG + chr4.2:39900219-39900238 MS.gene02469:CDS 60.0%
CAACCCGCTGCTGCAGTTCA+AGG + chr4.2:39903841-39903860 MS.gene02469:CDS 60.0%
CCGACGGGGCATAGTCATCA+TGG + chr4.2:39900155-39900174 MS.gene02469:CDS 60.0%
CTTTCTTGGGGTCGCAGTCC+AGG + chr4.2:39903255-39903274 MS.gene02469:CDS 60.0%
GAAACCGCCCACTGTGCTGT+GGG - chr4.2:39902930-39902949 None:intergenic 60.0%
GCAGCATCAGCAGAAGCAGC+TGG + chr4.2:39900202-39900221 MS.gene02469:CDS 60.0%
TGATGACTATGCCCCGTCGG+AGG - chr4.2:39900155-39900174 None:intergenic 60.0%
TGCAACCCCCACAGCACAGT+GGG + chr4.2:39902920-39902939 MS.gene02469:intron 60.0%
TGGGCTACATTCAGCGCTGC+CGG + chr4.2:39901199-39901218 MS.gene02469:CDS 60.0%
TTGCAACCCCCACAGCACAG+TGG + chr4.2:39902919-39902938 MS.gene02469:intron 60.0%
!! GCTGTAGTGTTGGCTGGTGG+CGG + chr4.2:39902568-39902587 MS.gene02469:intron 60.0%
!! TCCAATGGTGCTGTGGCCCA+AGG + chr4.2:39902598-39902617 MS.gene02469:CDS 60.0%
!! TGGCGAGAGGCGTTCTTCTG+AGG + chr4.2:39901228-39901247 MS.gene02469:CDS 60.0%
AACCCCCACAGCACAGTGGG+CGG + chr4.2:39902923-39902942 MS.gene02469:intron 65.0%
AACCGCCCACTGTGCTGTGG+GGG - chr4.2:39902928-39902947 None:intergenic 65.0%
ACATTCAGCGCTGCCGGTGG+TGG + chr4.2:39901205-39901224 MS.gene02469:CDS 65.0%
GCCCCTTGAACTGCAGCAGC+GGG - chr4.2:39903847-39903866 None:intergenic 65.0%
GCTACATTCAGCGCTGCCGG+TGG + chr4.2:39901202-39901221 MS.gene02469:CDS 65.0%
TGACTATGCCCCGTCGGAGG+CGG - chr4.2:39900152-39900171 None:intergenic 65.0%
! GGTGGCGGTGGACACTCCAA+TGG + chr4.2:39902583-39902602 MS.gene02469:CDS 65.0%
!! GTAGTGTTGGCTGGTGGCGG+TGG + chr4.2:39902571-39902590 MS.gene02469:CDS 65.0%
ACGCCTCTCGCCACCACCAC+CGG - chr4.2:39901221-39901240 None:intergenic 70.0%
! AAGTGTCGCCGCCTCCGACG+GGG + chr4.2:39900141-39900160 MS.gene02469:CDS 70.0%
! CAAGTGTCGCCGCCTCCGAC+GGG + chr4.2:39900140-39900159 MS.gene02469:CDS 70.0%
! GCAAGTGTCGCCGCCTCCGA+CGG + chr4.2:39900139-39900158 MS.gene02469:CDS 70.0%
! TTCAGCGCTGCCGGTGGTGG+TGG + chr4.2:39901208-39901227 MS.gene02469:CDS 70.0%
!! CTGCCGGTGGTGGTGGCGAG+AGG + chr4.2:39901215-39901234 MS.gene02469:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 39900017 39903873 39900017 ID=MS.gene02469
chr4.2 mRNA 39900017 39903873 39900017 ID=MS.gene02469.t1;Parent=MS.gene02469
chr4.2 exon 39900017 39900316 39900017 ID=MS.gene02469.t1.exon1;Parent=MS.gene02469.t1
chr4.2 CDS 39900017 39900316 39900017 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
chr4.2 exon 39901046 39901541 39901046 ID=MS.gene02469.t1.exon2;Parent=MS.gene02469.t1
chr4.2 CDS 39901046 39901541 39901046 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
chr4.2 exon 39902569 39902654 39902569 ID=MS.gene02469.t1.exon3;Parent=MS.gene02469.t1
chr4.2 CDS 39902569 39902654 39902569 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
chr4.2 exon 39902733 39902863 39902733 ID=MS.gene02469.t1.exon4;Parent=MS.gene02469.t1
chr4.2 CDS 39902733 39902863 39902733 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
chr4.2 exon 39903191 39903287 39903191 ID=MS.gene02469.t1.exon5;Parent=MS.gene02469.t1
chr4.2 CDS 39903191 39903287 39903191 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
chr4.2 exon 39903742 39903873 39903742 ID=MS.gene02469.t1.exon6;Parent=MS.gene02469.t1
chr4.2 CDS 39903742 39903873 39903742 ID=cds.MS.gene02469.t1;Parent=MS.gene02469.t1
Gene Sequence

>MS.gene02469

ATGTCTATGCAGTATCCAGCTACTGCTATGGCTATGATGCAACAGCAGATGATGATGTATCCTCAGCATTATATGCCTTATGTTCATCCTCATCATTATCAGCAGCAGCCACCTCATCAACAGCAAGTGTCGCCGCCTCCGACGGGGCATAGTCATCATGGAGGCCATCAGCAGCATCAATATCAGCAGCATCAGCAGAAGCAGCTGGCGCCTGAAGAGATCAGGACGATCTGGCTTGGTGACCTTCATCATTGGATGGATGAGACTTTTCTTCATAACTGCTTTGCTCATACTGGAGAGGTTTATTTCTATCTCTTTAATTGTAATTGAATTTGTTAGATTCGTTGTTTTAATTTCAATATTAATATGCTTCATTTGCTTAATTAATAGCATATCTTGTTAGTTTCTGTTTTTATATAAATGGTCTTGATTTATTGCTGGAAGTTATTAGGGTACAGGCTTCTTTGTTTAATTTGCTGATTTGAATTTTTTTTTTTTTTTGATTTGAGATTGATTTAAGGAGCCTTGATTTAATTAATCACTGAGGATTTGTTGAATCTTGATTAAATGTTTTAACTTTTAAGCTATGATTTCGAGTTTAATTTAAGTTCATCGATCTTTTTGTGTGTGATTATTGGACTCTTTAATGAATTAGGGTTGTAAATTGACTCTTGTGATTTAAGTTGGTTTCCTTTTTCTATCCTTGGCATTGCCCATATAAGATAGTTATACTTTTGGTACCCTAAGTGGTCCCCTAGAGTTTTGAAGGCCGAAAATGTGACCTTGTTGACCAATGTTTTTAACTAGACTTGCTAGTATAACCTAGATCTCTTAATTGCACTTTGAAAAATGGGGCTAATCAAAATTTGCTTTTTGTCCAATAATGTATTGTAGGATATTCACATGTTAAATATCAATAGCCGACTTTGACGCTTAGACATTGCCGATTATCGCATTTATGACCAAACTTTTTTTTTATTGTCGTCTTCTGATTGGTTTGTTGATGTTTTTTCGTTGGCTTATGTGCAGGTTGTATCGGCCAAGGTCATACGTAATAAGCAAACTGGTCAATCGGAGGGATATGGATTTGTCGAGTTCTATACGCGAGCAATGGCTGAGAAAGTCTTGCAGAACTTCAATGGTACTATGATGCCAAATACCGATCAGGCGTTTCGTCTGAATTGGGCTACATTCAGCGCTGCCGGTGGTGGTGGCGAGAGGCGTTCTTCTGAGGCTACATCTGATCTCTCTGTATTTGTGGGAGACTTGGCCATAGATGTTACTGATGCTATGCTTCAAGAGACGTTTGCTAGCAAATTTTCATCTATTAAGGGAGCAAAAGTTGTTATTGACTCTAATACTGGTCGTTCAAAAGGCTATGGTTTTGTTAGGTTTGGTGATGAAAGTGAAAGGACAAGAGCAATGACTGAAATGAATGGCGTTTATTGTTCTAGCAGGCCTATGCGAGTAGGTGTTGCAACACCCAAAAAAACTTATGGCAATCCACAACAATATTCTTCGCAAGGTTTGTTATTTAATTATTACCACTTAAATCAGCACCAACCTTTTCTGCTGCAGATACTTCAAATTTTCATTATAACTGAAAGGAGGTCCTAATAATTCAATTCCCTGGTTGGATTGATATTAATATTTTCTTTAATGATTTGTTTGATTTCATTTGTGCATATCTATTTTTATGTTGGTTAAAATTTGAATATAAACATGTATGATGAATAAATCGAAGTGGCCTGTTTTATTTGTAAGTGTGCTTTATGATTTCAGTAATCATACTTGACTTTTCAATACAAGTCTTGGAGCTTGTTGCATCTTAAATATGTTAATTCGTAAATATCATATTTGTTTCTGGCTTTATGCATGAATTTAAACATGTGTGATAAATAAATTGTGAAGAATAGTTATGCATGAATTCAAACATGGCTTTATGCATGAATATTTGTTTCTGGCTTTACCATAGATGTTTATTAAATATAAACATGTATGTTAATTTGTAAGTTAGCCTGTTTTATTTGTAAGTGCGCTTTAGTATTCATAGTTGACTTTTCAATACAAGTCTCTGAGCTTGTTACTTCTTAAATATGTTAATTCGTATATTAAATCTCATATTTGTTCCTGGCTTTATGCATTAATGGACTTGCGCTTTTTATTTTTGACAATTGCTTATTGCAGATTTTTCTATGCTTTTGCACCACTTTGTTATATTTCCTTGCACCACTTGAATCTATTCCCTTGCCTATCTCATGATGTATACTGTTTGATGTCTAAACGACAAGTTTGTATTTTTCAACCTCAATCTATTTATTGAATGTTAGTGATGTATGTTTATAGATTTTTTTTTTTTTTTTTTTTTGTAATTTAGAATCTTGAATGCATAACCAGGCAGAAAAATTAATTTTTATGGCATATATATTGCAAAGCTTTCTTGTTTATGTTTATGTTTATAACACGAGAGAGTTATCACTGGAATGCAGTAATATCACGTATCATGCTTCAGAATTTTAAATCACTGACATTTTTTTTCTTTCTTAAACTAGCTGTAGTGTTGGCTGGTGGCGGTGGACACTCCAATGGTGCTGTGGCCCAAGGTTCCCAATCTGAAGGGGATTCTAACAACACAACTGTGAGTCTGCTTTGGTAGCTTTTTCTATATCTTTTAATCAACTTCTTTTGATATCTCAGTAACGGATATCTGTTGCAGATATTTGTTGGAGGACTTGACTCTGACATTAGTGATGAGGATCTCAGACAACCATTTTTGCAATTTGGTGATGTAATCTCTGTGAAAATCCCAGTCGGTAAAGGATGTGGTTTTGTTCAACTTGCTGACAGGTACACTTGTGGTATCCCTTAGCATAGATATAGATCGTAACTTGCTTGCAAGTCATTGCAACCCCCACAGCACAGTGGGCGGTTTCTTGCGAAATGAACAGTGGCATAAGTATTCCTCAAATGGATAGGGAATCCTTGATTATGCCAACTCTTCATCCTGTGGAATCATGTAAGGATGAGGATATGATAATAAAGAAAGTTGATGTGAACTACTGTATAAGATGCTATGATTCTGATTGCAATTGTAACTGCCAATATGTATCGTCTTATTTTTGACTGGGTGGATTAGCTATGTTTTAATAATGCCTTCTGCTCTTTATGTTACAGAAAGAATGCTGAGGAAGCAATTCAGGGATTGAATGGAACAGTGATTGGAAAGCAGACAGTGCGTCTTTCTTGGGGTCGCAGTCCAGGAAATAAGCATGTGAGTTTATTATTCTGCTCTGAATTCAATTTGTTGTTATATTTTCACTTTGGCCATCTAATGGGAAAATTACGAGTCTCGTTCTCTCTGTAATCAGGTCCTGTTCTAAGTTTGTGTACTAAATCTGTGAAACTAATTGTTTTTAGTAGTGTAAGCAATACAGGCATCAGTGTACTAAATGTGTTGTATATTTTCTATTATGAGCTGCATCTTCTACTTACAAACCAAATTATTTTATAAAATTTCCGCATTCATATACCTTCAGCATGTAACTAGTTGCTACTAAGAGTTTTCAATTAGCATATACATACACTTTTTAATTTCTTTTCACCTTAGATTTTGATTATGAAGTCCCTAAAATGAATTCTCACTTTTAAGTAAAGCAGAAACCAATCAAGTAATCTTATATCATCATTTAAATCCATCTCTAATTTAATTCTTTGTGATGTCGCAGTGGAGGAATGACTCTAATGGTTATGGAGGTCATGGATATGGTGGCCATGGATATGGAAGTAATGGATATGCTGCTAGACAAAATCAGGATATAGCTATGCAACCCGCTGCTGCAGTTCAAGGGGCTTCCTAA
Protein sequence

>MS.gene02469.t1

MSMQYPATAMAMMQQQMMMYPQHYMPYVHPHHYQQQPPHQQQVSPPPTGHSHHGGHQQHQYQQHQQKQLAPEEIRTIWLGDLHHWMDETFLHNCFAHTGEVVSAKVIRNKQTGQSEGYGFVEFYTRAMAEKVLQNFNGTMMPNTDQAFRLNWATFSAAGGGGERRSSEATSDLSVFVGDLAIDVTDAMLQETFASKFSSIKGAKVVIDSNTGRSKGYGFVRFGDESERTRAMTEMNGVYCSSRPMRVGVATPKKTYGNPQQYSSQAVVLAGGGGHSNGAVAQGSQSEGDSNNTTIFVGGLDSDISDEDLRQPFLQFGDVISVKIPVGKGCGFVQLADRKNAEEAIQGLNGTVIGKQTVRLSWGRSPGNKHWRNDSNGYGGHGYGGHGYGSNGYAARQNQDIAMQPAAAVQGAS