AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023496


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023496.t1 MTR_8g081600 94.096 271 16 0 1 271 1 271 0.0 524
MS.gene023496.t1 MTR_2g036780 35.793 271 157 6 1 260 164 428 4.03e-42 149
MS.gene023496.t1 MTR_1g035890 32.857 280 171 6 1 268 165 439 1.94e-40 145
MS.gene023496.t1 MTR_3g100150 31.852 270 172 6 1 261 163 429 3.09e-39 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023496.t1 AT3G23840 38.806 268 147 8 1 266 164 416 6.47e-45 156
MS.gene023496.t1 AT4G13840 33.086 269 169 6 1 266 166 426 4.28e-42 149
MS.gene023496.t1 AT4G24510 30.038 263 167 6 1 260 168 416 3.22e-37 136

Find 75 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACTTGCAAGAACAAATT+TGG 0.140491 4.3:-15494821 MS.gene023496:CDS
TGACACTAGTTTGAAAATTT+TGG 0.151455 4.3:-15494601 MS.gene023496:CDS
GTCTCAACACCCCTTAATTT+TGG 0.157335 4.3:-15494960 MS.gene023496:CDS
AAACTTTATGTGCAATAATT+TGG 0.180735 4.3:-15494767 MS.gene023496:CDS
CTTCAGATTTCATCAACTTA+TGG 0.205690 4.3:-15495037 MS.gene023496:CDS
GTTCTTGCAAGTTGTTCATT+AGG 0.260077 4.3:+15494829 None:intergenic
TTCAGATTTCATCAACTTAT+GGG 0.298539 4.3:-15495036 MS.gene023496:CDS
TGGTAGGGGCAATGATATAA+TGG 0.301115 4.3:-15494676 MS.gene023496:CDS
TGATGAAAGAAATCAAATTA+TGG 0.305939 4.3:-15494553 MS.gene023496:CDS
ATATCATTGCCCCTACCATT+AGG 0.319594 4.3:+15494681 None:intergenic
TTCTTGCAAGTTGTTCATTA+GGG 0.331603 4.3:+15494830 None:intergenic
TGTGTATGACTTAAAATTAA+AGG 0.335448 4.3:-15494448 MS.gene023496:CDS
GGGAGATGAAGGTGTTGTTT+TGG 0.345479 4.3:-15494385 MS.gene023496:CDS
TTCATCTTCTGGCAATATAA+TGG 0.352304 4.3:+15494323 None:intergenic
TTGACCCGGTTGGTGATCAT+TGG 0.353117 4.3:-15494905 MS.gene023496:CDS
CAACTTGCAAGAACAAATTT+GGG 0.358829 4.3:-15494820 MS.gene023496:CDS
TATCATTGCCCCTACCATTA+GGG 0.360008 4.3:+15494682 None:intergenic
AGTGTGTAGTAAACAAATCT+TGG 0.364561 4.3:+15494417 None:intergenic
AACTGTCACGGTTGTGGGTT+CGG 0.365252 4.3:+15494716 None:intergenic
AGGAACCAGCTACATTTAAA+CGG 0.370152 4.3:-15494929 MS.gene023496:CDS
TAAGTCAAGAAATGTCAAAT+TGG 0.372854 4.3:+15494482 None:intergenic
AGGGGTGTTGAGACGGATCT+TGG 0.389004 4.3:+15494969 None:intergenic
TTTGTTTACTACACACTTCA+AGG 0.403996 4.3:-15494411 MS.gene023496:CDS
GTGTATGACTTAAAATTAAA+GGG 0.419239 4.3:-15494447 MS.gene023496:CDS
GTTCGGATCCTTCTCGAACT+CGG 0.421086 4.3:+15494733 None:intergenic
TCGACAAACTGTCACGGTTG+TGG 0.425168 4.3:+15494710 None:intergenic
GGTAGGGGCAATGATATAAT+GGG 0.427066 4.3:-15494675 MS.gene023496:CDS
TATATTGCCAGAAGATGAAA+TGG 0.440744 4.3:-15494319 MS.gene023496:CDS
ATTGCACATAAAGTTTCAAA+TGG 0.442525 4.3:+15494774 None:intergenic
CACATAAAGTTTCAAATGGA+GGG 0.442722 4.3:+15494778 None:intergenic
CGACAAACTGTCACGGTTGT+GGG 0.445047 4.3:+15494711 None:intergenic
GGAACCAGCTACATTTAAAC+GGG 0.448779 4.3:-15494928 MS.gene023496:CDS
CCTAAGGTGAATGATATTGA+TGG 0.452041 4.3:-15494357 MS.gene023496:CDS
TTCGGATCCTTCTCGAACTC+GGG 0.459230 4.3:+15494734 None:intergenic
CCTCAACCTTGCATATCAAT+TGG 0.459576 4.3:+15494646 None:intergenic
GCTAGCATGCTTGTGGATCA+AGG 0.466138 4.3:-15494579 MS.gene023496:CDS
AAATCCGAGCTTAATGTGTA+TGG 0.468103 4.3:-15494288 MS.gene023496:CDS
CTTCAAGGGGTGGGAGATGA+AGG 0.469388 4.3:-15494396 MS.gene023496:CDS
GTGTGTAGTAAACAAATCTT+GGG 0.472684 4.3:+15494418 None:intergenic
CCATCAATATCATTCACCTT+AGG 0.474137 4.3:+15494357 None:intergenic
GGGATCCAATGATCACCAAC+CGG 0.475921 4.3:+15494900 None:intergenic
GACGGATCTTGGAATGTTGA+AGG 0.485139 4.3:+15494980 None:intergenic
GGAGGGGTTTGTTCAACACT+TGG 0.494342 4.3:+15494795 None:intergenic
TACATTTAAACGGGTTGACC+CGG 0.497184 4.3:-15494919 MS.gene023496:CDS
GTTTGTCGACCCGACCCTAA+TGG 0.497568 4.3:-15494696 MS.gene023496:CDS
TCAACCCGTTTAAATGTAGC+TGG 0.502437 4.3:+15494924 None:intergenic
TTGATATGCAAGGTTGAGGC+AGG 0.515574 4.3:-15494642 MS.gene023496:CDS
GCACATAAAGTTTCAAATGG+AGG 0.536084 4.3:+15494777 None:intergenic
TACTACACACTTCAAGGGGT+GGG 0.539191 4.3:-15494405 MS.gene023496:CDS
TCGACCCGACCCTAATGGTA+GGG 0.545566 4.3:-15494691 MS.gene023496:CDS
TTGTTTACTACACACTTCAA+GGG 0.547045 4.3:-15494410 MS.gene023496:CDS
GTGTATGGTATCATGTTTGA+GGG 0.549776 4.3:-15494273 MS.gene023496:CDS
TTGCCCCTACCATTAGGGTC+GGG 0.551150 4.3:+15494687 None:intergenic
ACATAAAGTTTCAAATGGAG+GGG 0.558070 4.3:+15494779 None:intergenic
ATTGCCCCTACCATTAGGGT+CGG 0.561243 4.3:+15494686 None:intergenic
TGTGTATGGTATCATGTTTG+AGG 0.561998 4.3:-15494274 MS.gene023496:CDS
AAAATTAAGGGGTGTTGAGA+CGG 0.562724 4.3:+15494962 None:intergenic
CCAATTGATATGCAAGGTTG+AGG 0.574004 4.3:-15494646 MS.gene023496:CDS
TCAGATTTCATCAACTTATG+GGG 0.579204 4.3:-15495035 MS.gene023496:CDS
TCATTAGGGAGCTAGTTATG+TGG 0.584575 4.3:+15494844 None:intergenic
ATGAAATCTGAAGCTGAATG+TGG 0.593595 4.3:+15495047 None:intergenic
GTCGACCCGACCCTAATGGT+AGG 0.594252 4.3:-15494692 MS.gene023496:CDS
TTTAAACGGGTTGACCCGGT+TGG 0.594712 4.3:-15494915 MS.gene023496:CDS
GGATCCAATGATCACCAACC+GGG 0.601111 4.3:+15494901 None:intergenic
TTACTACACACTTCAAGGGG+TGG 0.606615 4.3:-15494406 MS.gene023496:CDS
CGACCCGACCCTAATGGTAG+GGG 0.607530 4.3:-15494690 MS.gene023496:CDS
ACGGATCTTGGAATGTTGAA+GGG 0.614481 4.3:+15494981 None:intergenic
TGTTTACTACACACTTCAAG+GGG 0.616578 4.3:-15494409 MS.gene023496:CDS
TAGGGAGCTAGTTATGTGGA+AGG 0.620633 4.3:+15494848 None:intergenic
TGCATAGCCCGAGTTCGAGA+AGG 0.625715 4.3:-15494741 MS.gene023496:CDS
GATACCATACACATTAAGCT+CGG 0.633143 4.3:+15494284 None:intergenic
AAATGACCAATTGATATGCA+AGG 0.646231 4.3:-15494652 MS.gene023496:CDS
AACTTGCAAGAACAAATTTG+GGG 0.669322 4.3:-15494819 MS.gene023496:CDS
GTCGGGTCGACAAACTGTCA+CGG 0.691405 4.3:+15494704 None:intergenic
TGAAATCTGAAGCTGAATGT+GGG 0.702970 4.3:+15495048 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTTATGTGCAATAATT+TGG - chr4.3:15494552-15494571 MS.gene023496:CDS 20.0%
!! ATATAGTAGAAAAAGATCAA+GGG - chr4.3:15494789-15494808 MS.gene023496:CDS 20.0%
!! GTGTATGACTTAAAATTAAA+GGG - chr4.3:15494872-15494891 MS.gene023496:CDS 20.0%
!! TGATGAAAGAAATCAAATTA+TGG - chr4.3:15494766-15494785 MS.gene023496:CDS 20.0%
!! TGTGTATGACTTAAAATTAA+AGG - chr4.3:15494871-15494890 MS.gene023496:CDS 20.0%
! ATTGCACATAAAGTTTCAAA+TGG + chr4.3:15494548-15494567 None:intergenic 25.0%
! GATATAGTAGAAAAAGATCA+AGG - chr4.3:15494788-15494807 MS.gene023496:CDS 25.0%
! TAAGTCAAGAAATGTCAAAT+TGG + chr4.3:15494840-15494859 None:intergenic 25.0%
! TATAGTAGAAAAAGATCAAG+GGG - chr4.3:15494790-15494809 MS.gene023496:CDS 25.0%
! TTCAGATTTCATCAACTTAT+GGG - chr4.3:15494283-15494302 MS.gene023496:CDS 25.0%
!!! TGACACTAGTTTGAAAATTT+TGG - chr4.3:15494718-15494737 MS.gene023496:CDS 25.0%
AAATGACCAATTGATATGCA+AGG - chr4.3:15494667-15494686 MS.gene023496:CDS 30.0%
AACTTGCAAGAACAAATTTG+GGG - chr4.3:15494500-15494519 MS.gene023496:CDS 30.0%
ACAACTTGCAAGAACAAATT+TGG - chr4.3:15494498-15494517 MS.gene023496:CDS 30.0%
ACATAAAGTTTCAAATGGAG+GGG + chr4.3:15494543-15494562 None:intergenic 30.0%
AGTGTGTAGTAAACAAATCT+TGG + chr4.3:15494905-15494924 None:intergenic 30.0%
CAACTTGCAAGAACAAATTT+GGG - chr4.3:15494499-15494518 MS.gene023496:CDS 30.0%
CACATAAAGTTTCAAATGGA+GGG + chr4.3:15494544-15494563 None:intergenic 30.0%
CTTCAGATTTCATCAACTTA+TGG - chr4.3:15494282-15494301 MS.gene023496:CDS 30.0%
GTGTGTAGTAAACAAATCTT+GGG + chr4.3:15494904-15494923 None:intergenic 30.0%
TATATTGCCAGAAGATGAAA+TGG - chr4.3:15495000-15495019 MS.gene023496:CDS 30.0%
TCAGATTTCATCAACTTATG+GGG - chr4.3:15494284-15494303 MS.gene023496:CDS 30.0%
TTCATCTTCTGGCAATATAA+TGG + chr4.3:15494999-15495018 None:intergenic 30.0%
TTCTTGCAAGTTGTTCATTA+GGG + chr4.3:15494492-15494511 None:intergenic 30.0%
TTGTTTACTACACACTTCAA+GGG - chr4.3:15494909-15494928 MS.gene023496:CDS 30.0%
TTTGTTTACTACACACTTCA+AGG - chr4.3:15494908-15494927 MS.gene023496:CDS 30.0%
! AGTTTTACCATTTCATCTTC+TGG + chr4.3:15495010-15495029 None:intergenic 30.0%
! CTTTTCGGATCCAAAATTAA+GGG + chr4.3:15494372-15494391 None:intergenic 30.0%
! TTTTAGGCTCAAATTGTTCA+AGG + chr4.3:15494315-15494334 None:intergenic 30.0%
! TTTTCGGATCCAAAATTAAG+GGG + chr4.3:15494371-15494390 None:intergenic 30.0%
!!! GTGACTGATTTTTTTGTCTA+TGG - chr4.3:15494812-15494831 MS.gene023496:CDS 30.0%
!!! TGTGTCCATTTTTTTGTTGT+TGG + chr4.3:15494447-15494466 None:intergenic 30.0%
AAATCCGAGCTTAATGTGTA+TGG - chr4.3:15495031-15495050 MS.gene023496:CDS 35.0%
AGGAACCAGCTACATTTAAA+CGG - chr4.3:15494390-15494409 MS.gene023496:CDS 35.0%
CCATCAATATCATTCACCTT+AGG + chr4.3:15494965-15494984 None:intergenic 35.0%
CCTAAGGTGAATGATATTGA+TGG - chr4.3:15494962-15494981 MS.gene023496:CDS 35.0%
GATACCATACACATTAAGCT+CGG + chr4.3:15495038-15495057 None:intergenic 35.0%
GCACATAAAGTTTCAAATGG+AGG + chr4.3:15494545-15494564 None:intergenic 35.0%
GTGTATGGTATCATGTTTGA+GGG - chr4.3:15495046-15495065 MS.gene023496:CDS 35.0%
GTTCTTGCAAGTTGTTCATT+AGG + chr4.3:15494493-15494512 None:intergenic 35.0%
TGTGTATGGTATCATGTTTG+AGG - chr4.3:15495045-15495064 MS.gene023496:CDS 35.0%
TGTTTACTACACACTTCAAG+GGG - chr4.3:15494910-15494929 MS.gene023496:CDS 35.0%
! ATGAAATCTGAAGCTGAATG+TGG + chr4.3:15494275-15494294 None:intergenic 35.0%
! CCTTAATTTTGGATCCGAAA+AGG - chr4.3:15494370-15494389 MS.gene023496:CDS 35.0%
! CCTTTTCGGATCCAAAATTA+AGG + chr4.3:15494373-15494392 None:intergenic 35.0%
! TGAAATCTGAAGCTGAATGT+GGG + chr4.3:15494274-15494293 None:intergenic 35.0%
!! AAAATTAAGGGGTGTTGAGA+CGG + chr4.3:15494360-15494379 None:intergenic 35.0%
!! AAATGTAGCTGGTTCCTTTT+CGG + chr4.3:15494387-15494406 None:intergenic 35.0%
!!! CCATTTTTTTGTTGTTGGCT+GGG + chr4.3:15494442-15494461 None:intergenic 35.0%
!!! TCCATTTTTTTGTTGTTGGC+TGG + chr4.3:15494443-15494462 None:intergenic 35.0%
ACGGATCTTGGAATGTTGAA+GGG + chr4.3:15494341-15494360 None:intergenic 40.0%
ATATCATTGCCCCTACCATT+AGG + chr4.3:15494641-15494660 None:intergenic 40.0%
CCAATTGATATGCAAGGTTG+AGG - chr4.3:15494673-15494692 MS.gene023496:CDS 40.0%
CCCAGCCAACAACAAAAAAA+TGG - chr4.3:15494439-15494458 MS.gene023496:CDS 40.0%
CCTCAACCTTGCATATCAAT+TGG + chr4.3:15494676-15494695 None:intergenic 40.0%
GGAACCAGCTACATTTAAAC+GGG - chr4.3:15494391-15494410 MS.gene023496:CDS 40.0%
GGTAGGGGCAATGATATAAT+GGG - chr4.3:15494644-15494663 MS.gene023496:CDS 40.0%
TATCATTGCCCCTACCATTA+GGG + chr4.3:15494640-15494659 None:intergenic 40.0%
TCAACCCGTTTAAATGTAGC+TGG + chr4.3:15494398-15494417 None:intergenic 40.0%
TCATTAGGGAGCTAGTTATG+TGG + chr4.3:15494478-15494497 None:intergenic 40.0%
TGGTAGGGGCAATGATATAA+TGG - chr4.3:15494643-15494662 MS.gene023496:CDS 40.0%
! GAATGTTGAAGGGCAGTTTT+AGG + chr4.3:15494331-15494350 None:intergenic 40.0%
! GTCTCAACACCCCTTAATTT+TGG - chr4.3:15494359-15494378 MS.gene023496:CDS 40.0%
!! TACATTTAAACGGGTTGACC+CGG - chr4.3:15494400-15494419 MS.gene023496:CDS 40.0%
!!! AATTTTGGCTAGCATGCTTG+TGG - chr4.3:15494733-15494752 MS.gene023496:CDS 40.0%
!!! TGTTGTTTTGGTGCTTCCTA+AGG - chr4.3:15494946-15494965 MS.gene023496:CDS 40.0%
GACGGATCTTGGAATGTTGA+AGG + chr4.3:15494342-15494361 None:intergenic 45.0%
TACTACACACTTCAAGGGGT+GGG - chr4.3:15494914-15494933 MS.gene023496:CDS 45.0%
TAGGGAGCTAGTTATGTGGA+AGG + chr4.3:15494474-15494493 None:intergenic 45.0%
TTACTACACACTTCAAGGGG+TGG - chr4.3:15494913-15494932 MS.gene023496:CDS 45.0%
TTGATATGCAAGGTTGAGGC+AGG - chr4.3:15494677-15494696 MS.gene023496:CDS 45.0%
!!! GGGAGATGAAGGTGTTGTTT+TGG - chr4.3:15494934-15494953 MS.gene023496:CDS 45.0%
AACTGTCACGGTTGTGGGTT+CGG + chr4.3:15494606-15494625 None:intergenic 50.0%
ATTGCCCCTACCATTAGGGT+CGG + chr4.3:15494636-15494655 None:intergenic 50.0%
CGACAAACTGTCACGGTTGT+GGG + chr4.3:15494611-15494630 None:intergenic 50.0%
GCTAGCATGCTTGTGGATCA+AGG - chr4.3:15494740-15494759 MS.gene023496:CDS 50.0%
GGATCCAATGATCACCAACC+GGG + chr4.3:15494421-15494440 None:intergenic 50.0%
GGGATCCAATGATCACCAAC+CGG + chr4.3:15494422-15494441 None:intergenic 50.0%
GTTCGGATCCTTCTCGAACT+CGG + chr4.3:15494589-15494608 None:intergenic 50.0%
TCGACAAACTGTCACGGTTG+TGG + chr4.3:15494612-15494631 None:intergenic 50.0%
TTCGGATCCTTCTCGAACTC+GGG + chr4.3:15494588-15494607 None:intergenic 50.0%
! GGAGGGGTTTGTTCAACACT+TGG + chr4.3:15494527-15494546 None:intergenic 50.0%
!! TTGACCCGGTTGGTGATCAT+TGG - chr4.3:15494414-15494433 MS.gene023496:CDS 50.0%
!! TTTAAACGGGTTGACCCGGT+TGG - chr4.3:15494404-15494423 MS.gene023496:CDS 50.0%
CTTCAAGGGGTGGGAGATGA+AGG - chr4.3:15494923-15494942 MS.gene023496:CDS 55.0%
GTCGGGTCGACAAACTGTCA+CGG + chr4.3:15494618-15494637 None:intergenic 55.0%
GTTTGTCGACCCGACCCTAA+TGG - chr4.3:15494623-15494642 MS.gene023496:CDS 55.0%
TCGACCCGACCCTAATGGTA+GGG - chr4.3:15494628-15494647 MS.gene023496:CDS 55.0%
TGCATAGCCCGAGTTCGAGA+AGG - chr4.3:15494578-15494597 MS.gene023496:CDS 55.0%
TTGCCCCTACCATTAGGGTC+GGG + chr4.3:15494635-15494654 None:intergenic 55.0%
!! AGGGGTGTTGAGACGGATCT+TGG + chr4.3:15494353-15494372 None:intergenic 55.0%
CGACCCGACCCTAATGGTAG+GGG - chr4.3:15494629-15494648 MS.gene023496:CDS 60.0%
GTCGACCCGACCCTAATGGT+AGG - chr4.3:15494627-15494646 MS.gene023496:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 15494263 15495078 15494263 ID=MS.gene023496
chr4.3 mRNA 15494263 15495078 15494263 ID=MS.gene023496.t1;Parent=MS.gene023496
chr4.3 exon 15494263 15495078 15494263 ID=MS.gene023496.t1.exon1;Parent=MS.gene023496.t1
chr4.3 CDS 15494263 15495078 15494263 ID=cds.MS.gene023496.t1;Parent=MS.gene023496.t1
Gene Sequence

>MS.gene023496

ATGGGGGACCCACATTCAGCTTCAGATTTCATCAACTTATGGGGTCAAACCTTGAACAATTTGAGCCTAAAACTGCCCTTCAACATTCCAAGATCCGTCTCAACACCCCTTAATTTTGGATCCGAAAAGGAACCAGCTACATTTAAACGGGTTGACCCGGTTGGTGATCATTGGATCCCAGCCAACAACAAAAAAATGGACACATTCTCCTTCCACATAACTAGCTCCCTAATGAACAACTTGCAAGAACAAATTTGGGGTCCAAGTGTTGAACAAACCCCTCCATTTGAAACTTTATGTGCAATAATTTGGCATTGCATAGCCCGAGTTCGAGAAGGATCCGAACCCACAACCGTGACAGTTTGTCGACCCGACCCTAATGGTAGGGGCAATGATATAATGGGAAATGACCAATTGATATGCAAGGTTGAGGCAGGAAAAGAGTGTTCTATTGTTGACACTAGTTTGAAAATTTTGGCTAGCATGCTTGTGGATCAAGGTATTGATGAAAGAAATCAAATTATGGATATAGTAGAAAAAGATCAAGGGGTGACTGATTTTTTTGTCTATGGTGCCAATTTGACATTTCTTGACTTAGAAGATGTCAATGTGTATGACTTAAAATTAAAGGGACAAAACCCAAGATTTGTTTACTACACACTTCAAGGGGTGGGAGATGAAGGTGTTGTTTTGGTGCTTCCTAAGGTGAATGATATTGATGGAAAATTTGTGACCATTATATTGCCAGAAGATGAAATGGTAAAACTTAAATCCGAGCTTAATGTGTATGGTATCATGTTTGAGGGTGACTTTTAA

Protein sequence

>MS.gene023496.t1

MGDPHSASDFINLWGQTLNNLSLKLPFNIPRSVSTPLNFGSEKEPATFKRVDPVGDHWIPANNKKMDTFSFHITSSLMNNLQEQIWGPSVEQTPPFETLCAIIWHCIARVREGSEPTTVTVCRPDPNGRGNDIMGNDQLICKVEAGKECSIVDTSLKILASMLVDQGIDERNQIMDIVEKDQGVTDFFVYGANLTFLDLEDVNVYDLKLKGQNPRFVYYTLQGVGDEGVVLVLPKVNDIDGKFVTIILPEDEMVKLKSELNVYGIMFEGDF