AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023608

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023608.t1 MTR_8g087430 98.140 215 2 1 1 213 1 215 2.46e-153 424
MS.gene023608.t1 MTR_7g056337 72.558 215 57 1 1 213 1 215 1.18e-109 313
MS.gene023608.t1 MTR_4g075550 35.526 228 126 10 2 213 47 269 2.55e-24 97.4
MS.gene023608.t1 MTR_8g012595 34.211 228 130 8 2 212 47 271 1.04e-23 95.5
MS.gene023608.t1 MTR_3g113920 32.751 229 131 9 2 213 47 269 3.15e-23 94.4
MS.gene023608.t1 MTR_8g012605 34.302 172 103 5 2 167 47 214 1.57e-21 89.7
MS.gene023608.t1 MTR_8g012615 33.333 198 115 7 30 213 10 204 2.80e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023608.t1 AT1G29330 81.395 215 38 1 1 213 1 215 1.99e-130 366
MS.gene023608.t1 AT3G25040 71.628 215 59 1 1 213 1 215 5.06e-113 322
MS.gene023608.t1 AT1G29330 84.314 153 24 0 61 213 1 153 2.32e-94 272
MS.gene023608.t1 AT3G25160 33.040 227 133 6 2 212 48 271 6.95e-25 99.0
MS.gene023608.t1 AT1G19970 34.061 229 128 10 2 213 47 269 3.82e-23 94.4
MS.gene023608.t1 AT2G21190 31.441 229 134 10 2 213 44 266 2.06e-22 92.0
MS.gene023608.t1 AT4G38790 33.188 229 130 10 2 213 48 270 2.29e-22 92.0
MS.gene023608.t1 AT1G75760 33.188 229 130 10 2 213 47 269 2.50e-22 92.0
MS.gene023608.t1 AT1G75760 33.645 214 120 9 2 199 47 254 1.11e-21 90.1
MS.gene023608.t1 AT1G19970 34.463 177 103 7 2 171 47 217 5.74e-19 82.4
MS.gene023608.t1 AT1G75760 33.143 175 104 7 2 169 47 215 1.01e-17 79.0

Find 35 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTCAATTTCAGATCTTC+TGG 0.214649 4.4:+13300569 MS.gene023608:intron
GGGCAATATGTATTCTTTCT+TGG 0.265304 4.4:+13300676 MS.gene023608:CDS
TGTGTTCTTAGCACGTTATT+TGG 0.272313 4.4:+13297542 MS.gene023608:CDS
TGTTGCTGCTAGCTTTCTTC+TGG 0.281140 4.4:+13297716 MS.gene023608:CDS
GGCAATATGTATTCTTTCTT+GGG 0.306579 4.4:+13300677 MS.gene023608:CDS
TCTTCTACTATTACTTCATC+AGG 0.375089 4.4:+13301263 MS.gene023608:CDS
GTGGATTATACATTCTCAAT+TGG 0.399809 4.4:+13301067 MS.gene023608:CDS
ATTGTGACCTGAACATGATT+TGG 0.429861 4.4:-13297407 None:intergenic
CTTCATAAACGTATTATAAA+CGG 0.434373 4.4:-13297586 None:intergenic
CTAGTTCGTTGGCTATTGTT+TGG 0.468529 4.4:+13297622 MS.gene023608:CDS
ACGTTATTTGGATCTGTTCA+CGG 0.477076 4.4:+13297554 MS.gene023608:CDS
ATGTCAATTTCAGATCTTCT+GGG 0.481234 4.4:+13300570 MS.gene023608:intron
GGAAATGTTGATAATTTGAC+TGG 0.481668 4.4:+13300655 MS.gene023608:CDS
GGCGAGTCTGAAGATGTTCA+TGG 0.501045 4.4:-13297329 None:intergenic
TTCGTTGCAACAAAACCAGC+TGG 0.501585 4.4:-13300630 None:intergenic
GTGCATGCGTGTTCATCCTA+TGG 0.502901 4.4:+13297644 MS.gene023608:CDS
TCATAGCTTCTCTTAACCAT+AGG 0.504328 4.4:-13297660 None:intergenic
CTGAACAACACCTGAGACAC+AGG 0.506289 4.4:-13301221 None:intergenic
CTGAGCCACGTTTCACTCGA+TGG 0.510830 4.4:+13301106 MS.gene023608:CDS
AGTTGCAATACTGCCCCAGC+TGG 0.517340 4.4:+13300615 MS.gene023608:CDS
AGTCTTCATTGCTAGTTCGT+TGG 0.524045 4.4:+13297611 MS.gene023608:CDS
CCACGTTTCACTCGATGGAT+AGG 0.526685 4.4:+13301111 MS.gene023608:CDS
GATAATGACGAAAAGTATCA+AGG 0.541133 4.4:-13297689 None:intergenic
AATCTTGAGATAATTCAAGC+AGG 0.588695 4.4:-13301396 None:intergenic
TTAAATTGCAGTGCGTATCG+TGG 0.598999 4.4:+13301048 MS.gene023608:intron
CGAGAAATTCACCTTCCTTG+AGG 0.604093 4.4:+13297752 MS.gene023608:CDS
TCGTTGCAACAAAACCAGCT+GGG 0.607236 4.4:-13300629 None:intergenic
ATGAACATCTTCAGACTCGC+CGG 0.613512 4.4:+13297331 MS.gene023608:CDS
ATAGAAAGGGACCTCAAGGA+AGG 0.624693 4.4:-13297763 None:intergenic
GAGGAGATGAGTCATGTCAC+CGG 0.626170 4.4:-13297350 None:intergenic
CCTATCCATCGAGTGAAACG+TGG 0.656144 4.4:-13301111 None:intergenic
CGTTGCAACAAAACCAGCTG+GGG 0.659984 4.4:-13300628 None:intergenic
GGAGGAGAACGAGAATGCTG+AGG 0.684833 4.4:-13297369 None:intergenic
AACGTGGCTCAGTCAAATAG+CGG 0.705507 4.4:-13301095 None:intergenic
GAAATGTTGATAATTTGACT+GGG 0.715465 4.4:+13300656 MS.gene023608:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTAGTATAATTTGAA+AGG + chr4.4:13300101-13300120 MS.gene023608:intron 10.0%
!! AATAAGGCTATAAAATAAAA+AGG + chr4.4:13298402-13298421 MS.gene023608:intron 15.0%
!! GATAATTATTAAAAGAAACA+TGG + chr4.4:13298491-13298510 MS.gene023608:intron 15.0%
!!! ATTTGAAAATGTTGTTTATT+TGG + chr4.4:13300858-13300877 MS.gene023608:intron 15.0%
!!! TTGATAATTGATATATGTAA+TGG + chr4.4:13300827-13300846 MS.gene023608:intron 15.0%
!! AGGTAAACAATATTTATTTG+TGG + chr4.4:13301283-13301302 MS.gene023608:intron 20.0%
!! ATAAGATTCATAGATAGAAA+GGG - chr4.4:13297779-13297798 None:intergenic 20.0%
!! ATTACAGTATGAGTAAAATA+AGG + chr4.4:13298386-13298405 MS.gene023608:intron 20.0%
!! ATTGTATGAAAGTAAACAAT+TGG - chr4.4:13298024-13298043 None:intergenic 20.0%
!! CACTGCAATTTAAAAATAAA+TGG - chr4.4:13301041-13301060 None:intergenic 20.0%
!! CTTCATAAACGTATTATAAA+CGG - chr4.4:13297589-13297608 None:intergenic 20.0%
!! GAAACTAACAAAAAAAAACA+GGG - chr4.4:13299657-13299676 None:intergenic 20.0%
!! GCATTAAATAGTTAGAAAAA+GGG - chr4.4:13298588-13298607 None:intergenic 20.0%
!! TGAAACTAACAAAAAAAAAC+AGG - chr4.4:13299658-13299677 None:intergenic 20.0%
!! TGAACAAATCAACTTAATTT+AGG - chr4.4:13299197-13299216 None:intergenic 20.0%
!! TGAATAAATAAAACAGAAAC+TGG - chr4.4:13298862-13298881 None:intergenic 20.0%
!! TGCATTAAATAGTTAGAAAA+AGG - chr4.4:13298589-13298608 None:intergenic 20.0%
!! TTAAAAAAATTCAAACAGGT+CGG - chr4.4:13298799-13298818 None:intergenic 20.0%
!! TTTAGTATAATTTGAAAGGA+TGG + chr4.4:13300105-13300124 MS.gene023608:intron 20.0%
!!! AGAACAAGTTTTTAATCAAA+TGG + chr4.4:13300076-13300095 MS.gene023608:intron 20.0%
!!! ATTTTTTTCTCTTCAATTTC+AGG + chr4.4:13297486-13297505 MS.gene023608:intron 20.0%
!!! GTTTTTTTTTGTTAGTTTCA+TGG + chr4.4:13299658-13299677 MS.gene023608:intron 20.0%
!!! TTAGTTCTATAAACCAATTT+TGG - chr4.4:13299631-13299650 None:intergenic 20.0%
!!! TTTTTATCGCAATTTCAAAA+GGG - chr4.4:13299555-13299574 None:intergenic 20.0%
!!! TTTTTTATCGCAATTTCAAA+AGG - chr4.4:13299556-13299575 None:intergenic 20.0%
! AAGATTTGAAAACAATTGAC+AGG - chr4.4:13300404-13300423 None:intergenic 25.0%
! AGAGAAAATACATTTCTTAG+AGG - chr4.4:13300805-13300824 None:intergenic 25.0%
! ATAAAGAAATGACTTTACAG+TGG - chr4.4:13299925-13299944 None:intergenic 25.0%
! ATAAAGCAGTCAAAACATAA+AGG - chr4.4:13300744-13300763 None:intergenic 25.0%
! ATATCTTTGTCCAAAATGTT+TGG - chr4.4:13300154-13300173 None:intergenic 25.0%
! ATATGATGATATGATCTCTT+GGG + chr4.4:13298259-13298278 MS.gene023608:intron 25.0%
! ATGAAATGAAACTTATCCAT+AGG - chr4.4:13299778-13299797 None:intergenic 25.0%
! CATAAGATTCATAGATAGAA+AGG - chr4.4:13297780-13297799 None:intergenic 25.0%
! GACTTAAAGTCATAACTATA+AGG - chr4.4:13299266-13299285 None:intergenic 25.0%
! TAAAAAAATTCAAACAGGTC+GGG - chr4.4:13298798-13298817 None:intergenic 25.0%
! TAAATAACCAATAACAGCAA+CGG - chr4.4:13299168-13299187 None:intergenic 25.0%
! TATATGATGATATGATCTCT+TGG + chr4.4:13298258-13298277 MS.gene023608:intron 25.0%
! TATGCATAAAAATCAACACT+CGG - chr4.4:13299343-13299362 None:intergenic 25.0%
! TATGCATTTGAAGGAATTAA+AGG - chr4.4:13299743-13299762 None:intergenic 25.0%
! TTAAAGTCATAACTATAAGG+TGG - chr4.4:13299263-13299282 None:intergenic 25.0%
! TTAGATGATTATGCATTTGA+AGG - chr4.4:13299752-13299771 None:intergenic 25.0%
! TTAGTGAAAGTGATGAATTA+GGG - chr4.4:13297454-13297473 None:intergenic 25.0%
! TTTACTAGTAATGACAAAGT+TGG + chr4.4:13300245-13300264 MS.gene023608:intron 25.0%
!! TTTTACTCATACTGTAATGT+TGG - chr4.4:13298384-13298403 None:intergenic 25.0%
!!! ACATTTCTAGATGATTGTTT+TGG + chr4.4:13298117-13298136 MS.gene023608:intron 25.0%
!!! GAAATGTTGATAATTTGACT+GGG + chr4.4:13300656-13300675 MS.gene023608:CDS 25.0%
!!! GAATTTTTTTAAAGCCTTTG+TGG + chr4.4:13298807-13298826 MS.gene023608:intron 25.0%
!!! GGCTTTAAAAAAATTCAAAC+AGG - chr4.4:13298803-13298822 None:intergenic 25.0%
!!! GTTGTATTTGCTATGATATA+TGG + chr4.4:13298092-13298111 MS.gene023608:intron 25.0%
!!! TGTGTTTTTGTTTTGAATGT+TGG + chr4.4:13299598-13299617 MS.gene023608:intron 25.0%
!!! TGTTTTTATTGTTTGGACTT+TGG + chr4.4:13298229-13298248 MS.gene023608:intron 25.0%
!!! TTTTTTGTTAGTTTCATGGT+TGG + chr4.4:13299662-13299681 MS.gene023608:intron 25.0%
AATCAGAATATCTCAAGAGT+AGG - chr4.4:13301183-13301202 None:intergenic 30.0%
AATGACTTTACAGTGGAAAA+AGG - chr4.4:13299918-13299937 None:intergenic 30.0%
ACAATTCATGTTATGAGTTC+AGG + chr4.4:13299394-13299413 MS.gene023608:intron 30.0%
ATAAAACTCATGCATTACCA+AGG - chr4.4:13299436-13299455 None:intergenic 30.0%
ATGACTTTACAGTGGAAAAA+GGG - chr4.4:13299917-13299936 None:intergenic 30.0%
ATGCATAGCTAAAAAAAAGG+AGG - chr4.4:13300363-13300382 None:intergenic 30.0%
ATGTCAATTTCAGATCTTCT+GGG + chr4.4:13300570-13300589 MS.gene023608:intron 30.0%
ATTGCAAACATAGAGAAGTA+GGG + chr4.4:13298320-13298339 MS.gene023608:intron 30.0%
CATAATCATCTAAAAGCCTA+TGG + chr4.4:13299759-13299778 MS.gene023608:intron 30.0%
GAAATAGAAATCATATCACC+AGG - chr4.4:13300300-13300319 None:intergenic 30.0%
GACTTGTGAAATTGAGTTAT+TGG + chr4.4:13299218-13299237 MS.gene023608:intron 30.0%
GGCAAAAAGCAATAAATGTT+GGG - chr4.4:13298567-13298586 None:intergenic 30.0%
GGCAATATGTATTCTTTCTT+GGG + chr4.4:13300677-13300696 MS.gene023608:CDS 30.0%
GTCCAAACAATAAAAACATC+AGG - chr4.4:13298227-13298246 None:intergenic 30.0%
GTGATGCATAGCTAAAAAAA+AGG - chr4.4:13300366-13300385 None:intergenic 30.0%
GTTAGTGAAAGTGATGAATT+AGG - chr4.4:13297455-13297474 None:intergenic 30.0%
GTTTACACAGAATCATGATT+GGG + chr4.4:13298692-13298711 MS.gene023608:intron 30.0%
TACTCTTGAGATATTCTGAT+TGG + chr4.4:13301182-13301201 MS.gene023608:intron 30.0%
TATGTCAATTTCAGATCTTC+TGG + chr4.4:13300569-13300588 MS.gene023608:intron 30.0%
TCTTCTACTATTACTTCATC+AGG + chr4.4:13301263-13301282 MS.gene023608:CDS 30.0%
TGTTTACACAGAATCATGAT+TGG + chr4.4:13298691-13298710 MS.gene023608:intron 30.0%
! AACAATTGACAGGTTTTCTA+TGG - chr4.4:13300394-13300413 None:intergenic 30.0%
! AGTGTTTAAGCCAAACATTT+TGG + chr4.4:13300141-13300160 MS.gene023608:intron 30.0%
! ATAGCAAAGCATTCCAAAAT+AGG - chr4.4:13299471-13299490 None:intergenic 30.0%
! GTGGATTATACATTCTCAAT+TGG + chr4.4:13301067-13301086 MS.gene023608:CDS 30.0%
! GTTTTACGATTTTACTACCT+TGG + chr4.4:13299416-13299435 MS.gene023608:intron 30.0%
! TGTTGTTTATTTGGCTGAAT+TGG + chr4.4:13300867-13300886 MS.gene023608:intron 30.0%
! TTTGTTCAGCTTTCCTATTT+TGG + chr4.4:13299455-13299474 MS.gene023608:intron 30.0%
!! AAAGGATTAGCTGAAAAAAC+AGG + chr4.4:13298420-13298439 MS.gene023608:intron 30.0%
!! GATAATGACGAAAAGTATCA+AGG - chr4.4:13297692-13297711 None:intergenic 30.0%
!! GGAAATGTTGATAATTTGAC+TGG + chr4.4:13300655-13300674 MS.gene023608:CDS 30.0%
!! GTCCTGATGTTTTTATTGTT+TGG + chr4.4:13298222-13298241 MS.gene023608:intron 30.0%
!!! AGATTCAGTTTTGAGATGAA+GGG - chr4.4:13298921-13298940 None:intergenic 30.0%
!!! AGGATTTTGGTTGTAAAAAC+AGG - chr4.4:13299079-13299098 None:intergenic 30.0%
!!! GGATTTTGGTTGTAAAAACA+GGG - chr4.4:13299078-13299097 None:intergenic 30.0%
AAAACAAGCAACAACAGAAG+AGG - chr4.4:13299099-13299118 None:intergenic 35.0%
AAATACAACACATCCTCGAA+TGG - chr4.4:13298081-13298100 None:intergenic 35.0%
AAATTCATGCCATCTTCTTC+TGG + chr4.4:13300209-13300228 MS.gene023608:intron 35.0%
AAGCAGTCAAAACATAAAGG+TGG - chr4.4:13300741-13300760 None:intergenic 35.0%
AATAGAATTCAGTACGGACA+GGG - chr4.4:13300448-13300467 None:intergenic 35.0%
ACAAAAATAGAGCACAAGAG+AGG - chr4.4:13298975-13298994 None:intergenic 35.0%
ACATTTCTTAGAGGACAATC+AGG - chr4.4:13300796-13300815 None:intergenic 35.0%
ACGTTATTTGGATCTGTTCA+CGG + chr4.4:13297554-13297573 MS.gene023608:CDS 35.0%
ATTGTGACCTGAACATGATT+TGG - chr4.4:13297410-13297429 None:intergenic 35.0%
CAAAAATAGAGCACAAGAGA+GGG - chr4.4:13298974-13298993 None:intergenic 35.0%
CATTGCAAACATAGAGAAGT+AGG + chr4.4:13298319-13298338 MS.gene023608:intron 35.0%
CTTAAACACTGCATGATACT+TGG - chr4.4:13300131-13300150 None:intergenic 35.0%
GCACGTAATAGAATTCAGTA+CGG - chr4.4:13300454-13300473 None:intergenic 35.0%
GGGCAAAAAGCAATAAATGT+TGG - chr4.4:13298568-13298587 None:intergenic 35.0%
GGGCAATATGTATTCTTTCT+TGG + chr4.4:13300676-13300695 MS.gene023608:CDS 35.0%
GTGGGATAAAATGAATTTCC+TGG + chr4.4:13300279-13300298 MS.gene023608:intron 35.0%
TAATAGAATTCAGTACGGAC+AGG - chr4.4:13300449-13300468 None:intergenic 35.0%
TACTGAAATCGGAAAGAACA+TGG - chr4.4:13298763-13298782 None:intergenic 35.0%
TAGGGATTGTGAAATGTGAA+GGG - chr4.4:13297436-13297455 None:intergenic 35.0%
TCATAGCTTCTCTTAACCAT+AGG - chr4.4:13297663-13297682 None:intergenic 35.0%
TCTTTCCGATTTCAGTAACT+GGG + chr4.4:13298766-13298785 MS.gene023608:intron 35.0%
TGTGTTCTTAGCACGTTATT+TGG + chr4.4:13297542-13297561 MS.gene023608:CDS 35.0%
TTAGGGATTGTGAAATGTGA+AGG - chr4.4:13297437-13297456 None:intergenic 35.0%
TTCTATTTCCTTCTTCCGTA+TGG + chr4.4:13300311-13300330 MS.gene023608:intron 35.0%
TTCTTTCCGATTTCAGTAAC+TGG + chr4.4:13298765-13298784 MS.gene023608:intron 35.0%
TTGCAAACATAGAGAAGTAG+GGG + chr4.4:13298321-13298340 MS.gene023608:intron 35.0%
! CTGGGCATTTTCAATATACT+TGG + chr4.4:13300588-13300607 MS.gene023608:CDS 35.0%
! GGCATTTTCAATATACTTGG+AGG + chr4.4:13300591-13300610 MS.gene023608:CDS 35.0%
!!! AAGGGAATTGGTTTTTAGCA+AGG - chr4.4:13298903-13298922 None:intergenic 35.0%
!!! AAGTGTTGGTGCTACATTTT+CGG + chr4.4:13297975-13297994 MS.gene023608:intron 35.0%
!!! AGGGAATTGGTTTTTAGCAA+GGG - chr4.4:13298902-13298921 None:intergenic 35.0%
!!! AGTTTTGAGATGAAGGGAAT+TGG - chr4.4:13298915-13298934 None:intergenic 35.0%
!!! ATTTGGTAGCGTAGATTTTG+AGG - chr4.4:13297393-13297412 None:intergenic 35.0%
!!! CAGATTCAGTTTTGAGATGA+AGG - chr4.4:13298922-13298941 None:intergenic 35.0%
!!! CAGGAATTTTGTTTTGCAGT+TGG + chr4.4:13301349-13301368 MS.gene023608:intron 35.0%
!!! CTGTTCTTTTCTAGAGTTCT+TGG - chr4.4:13299973-13299992 None:intergenic 35.0%
!!! TTCTAGATGATTGTTTTGGC+TGG + chr4.4:13298121-13298140 MS.gene023608:intron 35.0%
ACGGACAGGGAATATATTCT+TGG - chr4.4:13300435-13300454 None:intergenic 40.0%
AGGGATTGTGAAATGTGAAG+GGG - chr4.4:13297435-13297454 None:intergenic 40.0%
AGTCTTCATTGCTAGTTCGT+TGG + chr4.4:13297611-13297630 MS.gene023608:CDS 40.0%
ATAGATAGAAAGGGACCTCA+AGG - chr4.4:13297770-13297789 None:intergenic 40.0%
ATTTGAGTGACGCTTAACCT+AGG + chr4.4:13298178-13298197 MS.gene023608:intron 40.0%
CAGGGAATATATTCTTGGAC+AGG - chr4.4:13300430-13300449 None:intergenic 40.0%
CTTCTCTATGTTTGCAATGC+AGG - chr4.4:13298318-13298337 None:intergenic 40.0%
GTAAAAACAGGGTACAAGAG+AGG - chr4.4:13299067-13299086 None:intergenic 40.0%
TACGCTACCAAATCATGTTC+AGG + chr4.4:13297400-13297419 MS.gene023608:intron 40.0%
TCGTACAAGTCTACGATTTC+TGG + chr4.4:13298651-13298670 MS.gene023608:intron 40.0%
TCTGTCCCAGTTACTGAAAT+CGG - chr4.4:13298774-13298793 None:intergenic 40.0%
TTAAATTGCAGTGCGTATCG+TGG + chr4.4:13301048-13301067 MS.gene023608:intron 40.0%
TTTCATGGTTGGAGAGGAAT+GGG + chr4.4:13299673-13299692 MS.gene023608:intron 40.0%
TTTCGCAGATGCAAATGAGA+CGG - chr4.4:13298057-13298076 None:intergenic 40.0%
TTTGAGTGACGCTTAACCTA+GGG + chr4.4:13298179-13298198 MS.gene023608:intron 40.0%
! ATTTTATCCCACACTGTGAG+CGG - chr4.4:13300271-13300290 None:intergenic 40.0%
! CTAGTTCGTTGGCTATTGTT+TGG + chr4.4:13297622-13297641 MS.gene023608:CDS 40.0%
! GCAACAACAGAAGAGGATTT+TGG - chr4.4:13299092-13299111 None:intergenic 40.0%
! TCTAACTCCGTTGCTGTTAT+TGG + chr4.4:13299158-13299177 MS.gene023608:intron 40.0%
! TGTTAGTTTCATGGTTGGAG+AGG + chr4.4:13299667-13299686 MS.gene023608:intron 40.0%
! TGTTGGAGCATCTCCAAAAT+TGG + chr4.4:13299615-13299634 MS.gene023608:intron 40.0%
!! TCTTGGGCATTTTGTTTCGA+GGG + chr4.4:13298275-13298294 MS.gene023608:intron 40.0%
!!! AGTGTTGGTGCTACATTTTC+GGG + chr4.4:13297976-13297995 MS.gene023608:intron 40.0%
AACGTGGCTCAGTCAAATAG+CGG - chr4.4:13301098-13301117 None:intergenic 45.0%
AATCCATAACCCTGTTCCCT+AGG - chr4.4:13298198-13298217 None:intergenic 45.0%
AGCAGATTCCATACGGAAGA+AGG - chr4.4:13300322-13300341 None:intergenic 45.0%
ATAACCCTAGCCACCATTGT+TGG - chr4.4:13298350-13298369 None:intergenic 45.0%
ATAGAAAGGGACCTCAAGGA+AGG - chr4.4:13297766-13297785 None:intergenic 45.0%
ATGAACATCTTCAGACTCGC+CGG + chr4.4:13297331-13297350 MS.gene023608:CDS 45.0%
CGAGAAATTCACCTTCCTTG+AGG + chr4.4:13297752-13297771 MS.gene023608:CDS 45.0%
GAAGTAGGGGTAACCAACAA+TGG + chr4.4:13298334-13298353 MS.gene023608:intron 45.0%
GAATTGCAGCCAGAAGAAGA+TGG - chr4.4:13300221-13300240 None:intergenic 45.0%
GGAGACTGAACACTGTGATT+CGG + chr4.4:13299698-13299717 MS.gene023608:intron 45.0%
GTAACCAACAATGGTGGCTA+GGG + chr4.4:13298343-13298362 MS.gene023608:intron 45.0%
GTACAAGAGAGGATGTGTTC+TGG - chr4.4:13299056-13299075 None:intergenic 45.0%
GTTTCATGGTTGGAGAGGAA+TGG + chr4.4:13299672-13299691 MS.gene023608:intron 45.0%
TAACCTAGGGAACAGGGTTA+TGG + chr4.4:13298192-13298211 MS.gene023608:intron 45.0%
TAACGAATGCTGCGTTGAAG+TGG + chr4.4:13300520-13300539 MS.gene023608:intron 45.0%
TCGTTGCAACAAAACCAGCT+GGG - chr4.4:13300632-13300651 None:intergenic 45.0%
TGACACTGACAGTTTGACAC+TGG + chr4.4:13297878-13297897 MS.gene023608:intron 45.0%
TGTAGCACCAACACTTCTGA+TGG - chr4.4:13297971-13297990 None:intergenic 45.0%
TTCGTTGCAACAAAACCAGC+TGG - chr4.4:13300633-13300652 None:intergenic 45.0%
! CTTCTTCTGGCTGCAATTCA+TGG + chr4.4:13300222-13300241 MS.gene023608:intron 45.0%
!! ACTGGTCTTATTGCCAAACC+AGG - chr4.4:13298844-13298863 None:intergenic 45.0%
!! CTCTTGGGCATTTTGTTTCG+AGG + chr4.4:13298274-13298293 MS.gene023608:intron 45.0%
!! TCTACGATTTCTGGAGTGCT+TGG + chr4.4:13298660-13298679 MS.gene023608:intron 45.0%
!! TGGTAGCGTAGATTTTGAGG+AGG - chr4.4:13297390-13297409 None:intergenic 45.0%
!! TGTTGCTGCTAGCTTTCTTC+TGG + chr4.4:13297716-13297735 MS.gene023608:CDS 45.0%
!!! CTGGTTTTGTTGCAACGAAG+TGG + chr4.4:13300634-13300653 MS.gene023608:CDS 45.0%
AAGATCGCGTGAAATCGCTG+TGG + chr4.4:13298716-13298735 MS.gene023608:intron 50.0%
AGGTCAGAGTTCAGCCACAA+AGG - chr4.4:13298824-13298843 None:intergenic 50.0%
ATGGTTGGAGAGGAATGGGT+TGG + chr4.4:13299677-13299696 MS.gene023608:intron 50.0%
CAGCTGCAGCAGATTCCATA+CGG - chr4.4:13300329-13300348 None:intergenic 50.0%
CCACGTTTCACTCGATGGAT+AGG + chr4.4:13301111-13301130 MS.gene023608:CDS 50.0%
CCTATCCATCGAGTGAAACG+TGG - chr4.4:13301114-13301133 None:intergenic 50.0%
CGCGTAGAAGCAGAAGATGT+TGG - chr4.4:13299021-13299040 None:intergenic 50.0%
CGTTGCAACAAAACCAGCTG+GGG - chr4.4:13300631-13300650 None:intergenic 50.0%
CTGAACAACACCTGAGACAC+AGG - chr4.4:13301224-13301243 None:intergenic 50.0%
GACGCTTAACCTAGGGAACA+GGG + chr4.4:13298186-13298205 MS.gene023608:intron 50.0%
GAGGAGATGAGTCATGTCAC+CGG - chr4.4:13297353-13297372 None:intergenic 50.0%
GGTAACCAACAATGGTGGCT+AGG + chr4.4:13298342-13298361 MS.gene023608:intron 50.0%
GTAGGGGTAACCAACAATGG+TGG + chr4.4:13298337-13298356 MS.gene023608:intron 50.0%
GTGCATGCGTGTTCATCCTA+TGG + chr4.4:13297644-13297663 MS.gene023608:CDS 50.0%
TGACGCTTAACCTAGGGAAC+AGG + chr4.4:13298185-13298204 MS.gene023608:intron 50.0%
TTTGTGGCTGAACTCTGACC+TGG + chr4.4:13298823-13298842 MS.gene023608:intron 50.0%
! CACGCATCCATCAGAAGTGT+TGG + chr4.4:13297961-13297980 MS.gene023608:intron 50.0%
! CACTTCTGATGGATGCGTGT+TGG - chr4.4:13297960-13297979 None:intergenic 50.0%
! GTTTTTGCAGCCTGTGTCTC+AGG + chr4.4:13301211-13301230 MS.gene023608:intron 50.0%
AAAGTTGGCCGCTCACAGTG+TGG + chr4.4:13300260-13300279 MS.gene023608:intron 55.0%
AAGTTGGCCGCTCACAGTGT+GGG + chr4.4:13300261-13300280 MS.gene023608:intron 55.0%
AGTTGCAATACTGCCCCAGC+TGG + chr4.4:13300615-13300634 MS.gene023608:CDS 55.0%
CTGAGCCACGTTTCACTCGA+TGG + chr4.4:13301106-13301125 MS.gene023608:CDS 55.0%
GCATCTGCGAAAGCCATTCG+AGG + chr4.4:13298065-13298084 MS.gene023608:intron 55.0%
GGAGGAGAACGAGAATGCTG+AGG - chr4.4:13297372-13297391 None:intergenic 55.0%
GGCTGAACTCTGACCTGGTT+TGG + chr4.4:13298828-13298847 MS.gene023608:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 13297331 13301404 13297331 ID=MS.gene023608
chr4.4 mRNA 13297331 13301404 13297331 ID=MS.gene023608.t1;Parent=MS.gene023608
chr4.4 exon 13297331 13297415 13297331 ID=MS.gene023608.t1.exon1;Parent=MS.gene023608.t1
chr4.4 CDS 13297331 13297415 13297331 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
chr4.4 exon 13297508 13297773 13297508 ID=MS.gene023608.t1.exon2;Parent=MS.gene023608.t1
chr4.4 CDS 13297508 13297773 13297508 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
chr4.4 exon 13300583 13300698 13300583 ID=MS.gene023608.t1.exon3;Parent=MS.gene023608.t1
chr4.4 CDS 13300583 13300698 13300583 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
chr4.4 exon 13301059 13301132 13301059 ID=MS.gene023608.t1.exon4;Parent=MS.gene023608.t1
chr4.4 CDS 13301059 13301132 13301059 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
chr4.4 exon 13301221 13301284 13301221 ID=MS.gene023608.t1.exon5;Parent=MS.gene023608.t1
chr4.4 CDS 13301221 13301284 13301221 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
chr4.4 exon 13301368 13301404 13301368 ID=MS.gene023608.t1.exon6;Parent=MS.gene023608.t1
chr4.4 CDS 13301368 13301404 13301368 ID=cds.MS.gene023608.t1;Parent=MS.gene023608.t1
Gene Sequence

>MS.gene023608

ATGAACATCTTCAGACTCGCCGGTGACATGACTCATCTCCTCAGCATTCTCGTTCTCCTCCTCAAAATCTACGCTACCAAATCATGTTCAGGTCACAATTTCCCCTTCACATTTCACAATCCCTAATTCATCACTTTCACTAACAATTGATCATGATTTTTTTCTCTTCAATTTCAGGTGTTTCTCGTAAGACTCAAGAACTATACGCGATTGTGTTCTTAGCACGTTATTTGGATCTGTTCACGGATTTCATTTCCGTTTATAATACGTTTATGAAGATAGTCTTCATTGCTAGTTCGTTGGCTATTGTTTGGTGCATGCGTGTTCATCCTATGGTTAAGAGAAGCTATGATAAAGACCTTGATACTTTTCGTCATTATCTTCTTGTTGCTGCTAGCTTTCTTCTGGCACTCGTCTTGCACGAGAAATTCACCTTCCTTGAGGTCCCTTTCTATCTATGAATCTTATGTTTCTCTTTTTTAAGTATTTTGCAATGTTAATGTTTGAGTTGCGTGATTTAATTGTGTTTTATTTGTTTATTTTACACTGACACTGACAGTTTGACACTGGTAAAAATTTGATTAATTGAATTTTCTCACATGTCAGTGTCTATGATGTATCTGACACCAACACGCATCCATCAGAAGTGTTGGTGCTACATTTTCGGGTTTTTAATCGTTGAGCATTTCTCCAATTGTTTACTTTCATACAATTTTTATGTTTCCGTCTCATTTGCATCTGCGAAAGCCATTCGAGGATGTGTTGTATTTGCTATGATATATGGATACATTTCTAGATGATTGTTTTGGCTGGTCATGATGACAAACTTTAGATAAGCAAGAAACATATTTGAGTGACGCTTAACCTAGGGAACAGGGTTATGGATTTTTTGTCCTGATGTTTTTATTGTTTGGACTTTGGAGTTCTTATATGATGATATGATCTCTTGGGCATTTTGTTTCGAGGGATGATTCTTTTGTTAAACCTGCATTGCAAACATAGAGAAGTAGGGGTAACCAACAATGGTGGCTAGGGTTATATTTGCATCAACCAACATTACAGTATGAGTAAAATAAGGCTATAAAATAAAAAGGATTAGCTGAAAAAACAGGAAAAAACAATCAGTTTATTTCATTTTCATTTTTTGTCTATGTTTCATCGATAATTATTAAAAGAAACATGGTATTTTTTTTAATTATCTATAAAAACAAATCAACTGAAAATCAAACGCAACCCAACATTTATTGCTTTTTGCCCTTTTTCTAACTATTTAATGCACATAGTTATTGAAATCTCGATTTAAAACGAAAAATCGAAAAATCGTACAAGTCTACGATTTCTGGAGTGCTTGGTGATTTCATGTTTACACAGAATCATGATTGGGTAAGATCGCGTGAAATCGCTGTGGAAAAACATAAAGTTGTGCAATCCATGTTCTTTCCGATTTCAGTAACTGGGACAGATCCCGACCTGTTTGAATTTTTTTAAAGCCTTTGTGGCTGAACTCTGACCTGGTTTGGCAATAAGACCAGTTTCTGTTTTATTTATTCATACGCGTTGCGTCGTTTCCCTTGCTAAAAACCAATTCCCTTCATCTCAAAACTGAATCTGTAATGCTTTGTTTTTGTGAGCATAACTCACCCTCTCTTGTGCTCTATTTTTGTGTTCATGTGCTTTGTTTTTGCGACCAACATCTTCTGCTTCTACGCGCTGTTTTTGCAACCAGAACACATCCTCTCTTGTACCCTGTTTTTACAACCAAAATCCTCTTCTGTTGTTGCTTGTTTTTGCTGCTAAGTTCTGTTCTTAATTCGATTCATTAGAATGTCTAACTCCGTTGCTGTTATTGGTTATTTAAGCAAGCCTAAATTAAGTTGATTTGTTCATGACTTGTGAAATTGAGTTATTGGTTATTTGAGAACTTATTTACCACCTTATAGTTATGACTTTAAGTCTAATTAAGTATTTGAATCTGAAGACTTACTTTCACTTACTAATGTTAATTTATGCCGAGTGTTGATTTTTATGCATATTATATGAGTTTAATGAATTTGTAGAATCTTACAATTCATGTTATGAGTTCAGGTTTTACGATTTTACTACCTTGGTAATGCATGAGTTTTATTTGTTCAGCTTTCCTATTTTGGAATGCTTTGCTATGCAATTACATTTTTCTCTTTGAGATCAAATGCAAATATAAGTAAATCTTGACAAATTTCATCCCTTTTGAAATTGCGATAAAAAATGCGATGTTTCGAAAAATAATCTGTGTTTTTGTTTTGAATGTTGGAGCATCTCCAAAATTGGTTTATAGAACTAAAAACCCTGTTTTTTTTTGTTAGTTTCATGGTTGGAGAGGAATGGGTTGGAGACTGAACACTGTGATTCGGTTTAAGATCTGATTTTTGTCCTTTAATTCCTTCAAATGCATAATCATCTAAAAGCCTATGGATAAGTTTCATTTCATATTTTCATGCAAATTTTGCATATTTGAGCAAAGTTGATAGACATTAATATTCTATCAAATGTGTTGAATCTGACGATATTTCAAAACTTATCTGATCACTTGTGACATTCTTTAATCCCTTTTTCCACTGTAAAGTCATTTCTTTATATGTTTGAACGTTGCTCATAGAAAACCAAGAACTCTAGAAAAGAACAGCTTCAGATATGTTGAGTGTTTCTTAATGTCAAGTGTCTCTGAACATCTTGATTATTTGTGTCAAATCTTGTAACATGTTAACAAGAACAAGTTTTTAATCAAATGGTTTTATTTTAGTATAATTTGAAAGGATGGCCAAGTATCATGCAGTGTTTAAGCCAAACATTTTGGACAAAGATATATTTTGTTGATAGTTTGAAATATTTAACATTTATAAAATTCATGCCATCTTCTTCTGGCTGCAATTCATGGTTTACTAGTAATGACAAAGTTGGCCGCTCACAGTGTGGGATAAAATGAATTTCCTGGTGATATGATTTCTATTTCCTTCTTCCGTATGGAATCTGCTGCAGCTGTGATTTATTTTCCTCCTTTTTTTTAGCTATGCATCACAAAAGCCATAGAAAACCTGTCAATTGTTTTCAAATCTTATTCCTGTCCAAGAATATATTCCCTGTCCGTACTGAATTCTATTACGTGCGCTACATGTTAGCTTTATAGTAATTCTGTTACACAATGTAACATAATAACGAATGCTGCGTTGAAGTGGAAATGCTCAATATACTTTGTTTTATGTATGTCAATTTCAGATCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATACTGCCCCAGCTGGTTTTGTTGCAACGAAGTGGAAATGTTGATAATTTGACTGGGCAATATGTATTCTTTCTTGGGTATGTGCTCTTTTGTTTATTTAGCTTCTCTTTTACGATCCACCTTTATGTTTTGACTGCTTTATACTATGAACTTTCTAGATACTTTTGTCATCCTGATTGTCCTCTAAGAAATGTATTTTCTCTACTTGATAATTGATATATGTAATGGTTGTCATTATTTGAAAATGTTGTTTATTTGGCTGAATTGGCATTTTTCTGCTGACATGTTGTCTAACAACTTGTCTCTAGTCTTCATGACTGCTTAGTTTCATGAGCAGTTTGCTGTAGAACTTTTAAATATTACTAGACAATCTGATCTTGTTCAAAATCTGTTTATTATGATAAGTAAATATCATACCATTTATTTTTAAATTGCAGTGCGTATCGTGGATTATACATTCTCAATTGGATATACCGCTATTTGACTGAGCCACGTTTCACTCGATGGATAGGTAAATATGCTTAAAATCAAAATATGAATTGCTATTTAAACTATATGCCTACTCTTGAGATATTCTGATTGGCTTCTTGTTTTTGCAGCCTGTGTCTCAGGTGTTGTTCAGACAGCTCTGTATGCTGATTTCTTCTACTATTACTTCATCAGGTAAACAATATTTATTTGTGGAAGTTCATGTTTTTATTATACCGATACAACAAATAGGTAACATCAGGAATTTTGTTTTGCAGTTGGAAGAACAATTCAAAACTGAAGCTGCCTGCTTGA
Protein sequence

>MS.gene023608.t1

MNIFRLAGDMTHLLSILVLLLKIYATKSCVSRKTQELYAIVFLARYLDLFTDFISVYNTFMKIVFIASSLAIVWCMRVHPMVKRSYDKDLDTFRHYLLVAASFLLALVLHEKFTFLEIFWAFSIYLEAVAILPQLVLLQRSGNVDNLTGQYVFFLGAYRGLYILNWIYRYLTEPRFTRWIACVSGVVQTALYADFFYYYFISWKNNSKLKLPA