AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68324.t1 MTR_5g035190 98.605 215 3 0 61 275 3 217 1.79e-161 447
MS.gene68324.t1 MTR_5g035190 98.605 215 3 0 61 275 57 271 1.56e-160 447
MS.gene68324.t1 MTR_8g063210 90.233 215 21 0 61 275 57 271 9.56e-149 417
MS.gene68324.t1 MTR_6g039970 82.326 215 38 0 61 275 57 271 4.47e-135 382
MS.gene68324.t1 MTR_4g102060 44.878 205 113 0 71 275 85 289 7.07e-63 200
MS.gene68324.t1 MTR_7g074900 46.369 179 96 0 86 264 100 278 3.36e-58 187
MS.gene68324.t1 MTR_5g040320 28.571 224 146 8 51 267 37 253 6.53e-14 70.1
MS.gene68324.t1 MTR_1g097310 33.094 139 80 5 128 259 110 242 1.26e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68324.t1 AT2G29390 81.905 210 38 0 54 263 9 218 8.76e-132 372
MS.gene68324.t1 AT2G29390 83.744 203 33 0 61 263 50 252 4.57e-131 372
MS.gene68324.t1 AT2G29390 83.744 203 33 0 61 263 57 259 6.53e-131 371
MS.gene68324.t1 AT1G07420 79.263 217 45 0 48 264 6 222 6.13e-129 365
MS.gene68324.t1 AT1G07420 82.843 204 35 0 61 264 57 260 1.21e-128 366
MS.gene68324.t1 AT2G29390 79.535 215 32 1 61 263 57 271 9.30e-128 364
MS.gene68324.t1 AT2G29390 85.795 176 25 0 88 263 1 176 3.38e-115 328
MS.gene68324.t1 AT2G29390 85.795 176 25 0 88 263 1 176 3.38e-115 328
MS.gene68324.t1 AT4G12110 44.608 204 113 0 71 274 87 290 7.38e-63 200
MS.gene68324.t1 AT4G22755 45.098 204 109 1 71 274 86 286 5.46e-61 195
MS.gene68324.t1 AT4G22756 40.171 234 134 1 47 274 57 290 7.65e-61 194
MS.gene68324.t1 AT1G69640 34.091 176 96 8 108 269 91 260 7.35e-14 70.1
MS.gene68324.t1 AT1G14290 29.756 205 127 8 56 251 38 234 8.67e-13 67.0
MS.gene68324.t1 AT1G14290 31.395 172 103 7 111 272 19 185 1.39e-12 65.1

Find 71 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCATGTATTAGGATATT+TGG 0.108814 5.2:+25674687 MS.gene68324:intron
TCAAACTTCTTACCTTTATA+TGG 0.220586 5.2:+25674095 MS.gene68324:CDS
GGAGGACTTTATATTCTATT+GGG 0.240593 5.2:+25673741 MS.gene68324:CDS
ATACATTTATCCTGAGCTTC+AGG 0.260988 5.2:-25673469 None:intergenic
TGGAGGACTTTATATTCTAT+TGG 0.278117 5.2:+25673740 MS.gene68324:CDS
ACTTGATAACTTTGTGGTTA+TGG 0.284408 5.2:+25674009 MS.gene68324:CDS
CTTGGGTTTGCAACCATTGT+TGG 0.325863 5.2:+25673966 MS.gene68324:CDS
CGCCTGTTGCTATATCATTT+CGG 0.345848 5.2:+25673496 MS.gene68324:CDS
ACAGGATATTGCACACAAAA+TGG 0.349309 5.2:+25673767 MS.gene68324:CDS
ATTGTTGGTCCTGCCATCAC+TGG 0.360355 5.2:+25673981 MS.gene68324:CDS
TGAAGGTATGCGACACCATT+TGG 0.370092 5.2:+25673903 MS.gene68324:intron
AAACCCAAGGAAAAGTATTT+CGG 0.381254 5.2:-25673952 None:intergenic
CCGCATCATGTGACGTGAAC+TGG 0.388262 5.2:-25673179 None:intergenic
AAACTAAAACGACCTACATT+TGG 0.389659 5.2:-25673156 None:intergenic
CGTTTGTTGTACACTAAGTC+CGG 0.404710 5.2:+25674245 MS.gene68324:CDS
GAGACTGACCCGCCATATAA+AGG 0.405139 5.2:-25674107 None:intergenic
GGCCCCACTTGATAACTTTG+TGG 0.415251 5.2:+25674003 MS.gene68324:CDS
AGGATATTTGGAACTGATGT+AGG 0.447738 5.2:+25674699 MS.gene68324:intron
TTGAGGGACTCCATGGGAAA+TGG 0.456424 5.2:-25674076 None:intergenic
AAATAATAGCCCTGAAGCTC+AGG 0.462452 5.2:+25673459 MS.gene68324:CDS
CACCGAAATGATATAGCAAC+AGG 0.465624 5.2:-25673498 None:intergenic
GAATGTTGATGAATAGTTCC+CGG 0.467989 5.2:-25674264 None:intergenic
ATATCCCGTGTTCAAATACA+TGG 0.472596 5.2:+25673546 MS.gene68324:CDS
TACATTTATCCTGAGCTTCA+GGG 0.477416 5.2:-25673468 None:intergenic
CTAAAACGACCTACATTTGG+AGG 0.478350 5.2:-25673153 None:intergenic
TTCATCAACATTCACTTACA+TGG 0.481785 5.2:+25674274 MS.gene68324:CDS
TGTGTGAAAGGCCAACTAAA+AGG 0.490513 5.2:-25673123 None:intergenic
TATAAAGGTAAGAAGTTTGA+GGG 0.492510 5.2:-25674092 None:intergenic
TTGTTGGTCCTGCCATCACT+GGG 0.499360 5.2:+25673982 MS.gene68324:CDS
ACTTCTTACCTTTATATGGC+GGG 0.503573 5.2:+25674099 MS.gene68324:CDS
GCCCTCTCAATCCATACAAA+AGG 0.506678 5.2:-25672612 None:intergenic
GTGAAAGTACCAGGATGGTA+GGG 0.507784 5.2:-25673587 None:intergenic
GACACCATTTGGACTTACTT+CGG 0.518283 5.2:+25673914 MS.gene68324:CDS
AAGAAGTTTGAGGGACTCCA+TGG 0.521768 5.2:-25674083 None:intergenic
TATTCCGAAGTAAGTCCAAA+TGG 0.522798 5.2:-25673918 None:intergenic
GGAGGGAAGTATTGTGTGAA+AGG 0.525704 5.2:-25673135 None:intergenic
ATCCACACTGCATGTTGCTG+TGG 0.531734 5.2:+25673227 MS.gene68324:CDS
GGTGAAAGTACCAGGATGGT+AGG 0.535188 5.2:-25673588 None:intergenic
ATATAAAGGTAAGAAGTTTG+AGG 0.535634 5.2:-25674093 None:intergenic
CAACATTCACTTACATGGAC+CGG 0.539104 5.2:+25674279 MS.gene68324:CDS
TATCAAGTGGGGCCCAGTGA+TGG 0.541295 5.2:-25673994 None:intergenic
GAGGACTTTATATTCTATTG+GGG 0.543637 5.2:+25673742 MS.gene68324:CDS
AAGTGGGGCCCAGTGATGGC+AGG 0.544565 5.2:-25673990 None:intergenic
GTAATCTTCCCCTACCATCC+TGG 0.549586 5.2:+25673578 MS.gene68324:CDS
CCAGTTCACGTCACATGATG+CGG 0.552009 5.2:+25673179 MS.gene68324:CDS
ATTGCGGTTACCATTTCCCA+TGG 0.553896 5.2:+25674066 MS.gene68324:CDS
AACTTCTTACCTTTATATGG+CGG 0.558008 5.2:+25674098 MS.gene68324:CDS
GATAACTTTGTGGTTATGGA+TGG 0.559169 5.2:+25674013 MS.gene68324:CDS
CCAAGGAAAAGTATTTCGGC+AGG 0.562424 5.2:-25673948 None:intergenic
TGATGCGGCTGCAATATTTG+TGG 0.564540 5.2:+25673194 MS.gene68324:CDS
TTTGTATGGATTGAGAGGGC+AGG 0.573114 5.2:+25672615 MS.gene68324:CDS
CAATACTTCCCTCCAAATGT+AGG 0.573201 5.2:+25673144 MS.gene68324:CDS
CATGCCCATGTATTTGAACA+CGG 0.585516 5.2:-25673551 None:intergenic
TGAAAGTACCAGGATGGTAG+GGG 0.586304 5.2:-25673586 None:intergenic
CAACAATGGTTGCAAACCCA+AGG 0.591178 5.2:-25673965 None:intergenic
GTTTGTTGTACACTAAGTCC+GGG 0.594576 5.2:+25674246 MS.gene68324:CDS
AGAAGGTGAAAGTACCAGGA+TGG 0.634868 5.2:-25673592 None:intergenic
AGTGATGGCAGGACCAACAA+TGG 0.638026 5.2:-25673979 None:intergenic
TAACCACAAAGTTATCAAGT+GGG 0.641518 5.2:-25674006 None:intergenic
AGAAGTTTGAGGGACTCCAT+GGG 0.642178 5.2:-25674082 None:intergenic
TAAAACGACCTACATTTGGA+GGG 0.644044 5.2:-25673152 None:intergenic
GAGACAGTTGAGGCACATTG+CGG 0.646359 5.2:+25674050 MS.gene68324:CDS
ATAACCACAAAGTTATCAAG+TGG 0.646385 5.2:-25674007 None:intergenic
ATGCCCATGTATTTGAACAC+GGG 0.648181 5.2:-25673550 None:intergenic
AGGACTTTATATTCTATTGG+GGG 0.650421 5.2:+25673743 MS.gene68324:CDS
ACAAACGATGATGATAATCG+TGG 0.659889 5.2:-25674229 None:intergenic
AACCACAAAGTTATCAAGTG+GGG 0.661563 5.2:-25674005 None:intergenic
GAGAGTCTTAGAGACAGTTG+AGG 0.667372 5.2:+25674040 MS.gene68324:CDS
TATCCCGTGTTCAAATACAT+GGG 0.696685 5.2:+25673547 MS.gene68324:CDS
AACCACAGCAACATGCAGTG+TGG 0.700563 5.2:-25673229 None:intergenic
CTCACTCGTGATGTACACTG+TGG 0.757598 5.2:-25673804 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATGTTTTACTTCATTT+TGG + chr5.2:25673720-25673739 MS.gene68324:CDS 15.0%
!!! ATTTACTTCATTTTTTATGA+TGG + chr5.2:25674598-25674617 MS.gene68324:intron 15.0%
!! ACTAATGAAAGATATATTCA+AGG - chr5.2:25674432-25674451 None:intergenic 20.0%
!! AGTTCTACATATAAATCTAT+TGG + chr5.2:25673405-25673424 MS.gene68324:intron 20.0%
!! TTACTTAACAAGATGAAATT+TGG + chr5.2:25673671-25673690 MS.gene68324:intron 20.0%
!!! GTTCTTTTTTTGCAAATATT+TGG + chr5.2:25673630-25673649 MS.gene68324:intron 20.0%
!!! GTTGTTCTAATTATTACTTT+TGG + chr5.2:25673087-25673106 MS.gene68324:intron 20.0%
!!! TTGTAATTTTGATACATTCT+AGG - chr5.2:25674371-25674390 None:intergenic 20.0%
! AAAATATGTTTGATGCTTAC+CGG - chr5.2:25674301-25674320 None:intergenic 25.0%
! AAATCAGATCTGAAAAATTG+AGG - chr5.2:25674209-25674228 None:intergenic 25.0%
! AAATGAAGTAAATCGTCAAT+TGG - chr5.2:25674591-25674610 None:intergenic 25.0%
! AATGAAGTAAATCGTCAATT+GGG - chr5.2:25674590-25674609 None:intergenic 25.0%
! ATATAAAGGTAAGAAGTTTG+AGG - chr5.2:25674096-25674115 None:intergenic 25.0%
! ATCATCTATGAACTTCTATA+GGG + chr5.2:25673867-25673886 MS.gene68324:intron 25.0%
! GGATATGAAAAAATCATAAC+AGG - chr5.2:25673532-25673551 None:intergenic 25.0%
! TATAAAGGTAAGAAGTTTGA+GGG - chr5.2:25674095-25674114 None:intergenic 25.0%
! TATGTATTTACATGTGAAAG+AGG - chr5.2:25673386-25673405 None:intergenic 25.0%
! TCAAACTTCTTACCTTTATA+TGG + chr5.2:25674095-25674114 MS.gene68324:CDS 25.0%
! TTGTGTAATACATACAGATA+AGG - chr5.2:25673046-25673065 None:intergenic 25.0%
! TTTCCATGTATTAGGATATT+TGG + chr5.2:25674687-25674706 MS.gene68324:intron 25.0%
!! AATGTTTTACTTCATTTTGG+AGG + chr5.2:25673723-25673742 MS.gene68324:CDS 25.0%
!! ATCAAACATATTTTCCCTTA+AGG + chr5.2:25674307-25674326 MS.gene68324:intron 25.0%
!! ATTACATCTCTTGTTGTATT+TGG + chr5.2:25672734-25672753 MS.gene68324:intron 25.0%
!!! CTGAATTTTTTCCATGTATT+AGG + chr5.2:25674679-25674698 MS.gene68324:intron 25.0%
!!! TGTTACGTTTTTAGAGTAAA+AGG + chr5.2:25674335-25674354 MS.gene68324:intron 25.0%
!!! TTTTTTTCCTTCTCATTTTG+AGG + chr5.2:25673288-25673307 MS.gene68324:intron 25.0%
AAACGTAACAAGTATCCTTA+AGG - chr5.2:25674325-25674344 None:intergenic 30.0%
AAACTAAAACGACCTACATT+TGG - chr5.2:25673159-25673178 None:intergenic 30.0%
AACGTAACAAGTATCCTTAA+GGG - chr5.2:25674324-25674343 None:intergenic 30.0%
AACTAGCTGATATTAAGCAA+AGG - chr5.2:25674144-25674163 None:intergenic 30.0%
AACTTCTTACCTTTATATGG+CGG + chr5.2:25674098-25674117 MS.gene68324:CDS 30.0%
AATGAACATTTGTAATGCCT+TGG + chr5.2:25674629-25674648 MS.gene68324:intron 30.0%
AGATAAGGAAGAAGTTGAAA+AGG - chr5.2:25673031-25673050 None:intergenic 30.0%
AGGAAGAATCTACAACATAA+AGG - chr5.2:25672695-25672714 None:intergenic 30.0%
AGGACTTTATATTCTATTGG+GGG + chr5.2:25673743-25673762 MS.gene68324:CDS 30.0%
ATAACCACAAAGTTATCAAG+TGG - chr5.2:25674010-25674029 None:intergenic 30.0%
CTGATATTTGACAACACTAA+AGG - chr5.2:25672867-25672886 None:intergenic 30.0%
CTGTATGTATTACACAACAT+AGG + chr5.2:25673049-25673068 MS.gene68324:intron 30.0%
GAGATGTAATTCATAACAGT+AGG - chr5.2:25672724-25672743 None:intergenic 30.0%
GAGGACTTTATATTCTATTG+GGG + chr5.2:25673742-25673761 MS.gene68324:CDS 30.0%
GATAAGGAAGAAGTTGAAAA+GGG - chr5.2:25673030-25673049 None:intergenic 30.0%
GATCATCTATGAACTTCTAT+AGG + chr5.2:25673866-25673885 MS.gene68324:intron 30.0%
GGAGGACTTTATATTCTATT+GGG + chr5.2:25673741-25673760 MS.gene68324:CDS 30.0%
GTTCCAAATATCCTAATACA+TGG - chr5.2:25674693-25674712 None:intergenic 30.0%
TAACCACAAAGTTATCAAGT+GGG - chr5.2:25674009-25674028 None:intergenic 30.0%
TAATTTCCTGTCAGTATTGT+GGG - chr5.2:25673353-25673372 None:intergenic 30.0%
TGGAGGACTTTATATTCTAT+TGG + chr5.2:25673740-25673759 MS.gene68324:CDS 30.0%
TTCATAACAGTAGGAACATA+AGG - chr5.2:25672715-25672734 None:intergenic 30.0%
TTCATCAACATTCACTTACA+TGG + chr5.2:25674274-25674293 MS.gene68324:CDS 30.0%
TTTGCTTAATATCAGCTAGT+TGG + chr5.2:25674143-25674162 MS.gene68324:intron 30.0%
! AATTGTGGTTGTGGATTTTA+TGG + chr5.2:25672895-25672914 MS.gene68324:intron 30.0%
! ACTTGATAACTTTGTGGTTA+TGG + chr5.2:25674009-25674028 MS.gene68324:CDS 30.0%
! TAGTAAAGCATCAAATTCCA+AGG - chr5.2:25674649-25674668 None:intergenic 30.0%
! TTACATGTGTTCTGCATTTT+TGG + chr5.2:25673832-25673851 MS.gene68324:intron 30.0%
!! AAACCCAAGGAAAAGTATTT+CGG - chr5.2:25673955-25673974 None:intergenic 30.0%
!!! AAGATGAAATTTGGTTTTGC+AGG + chr5.2:25673680-25673699 MS.gene68324:intron 30.0%
AACCACAAAGTTATCAAGTG+GGG - chr5.2:25674008-25674027 None:intergenic 35.0%
ACAAACGATGATGATAATCG+TGG - chr5.2:25674232-25674251 None:intergenic 35.0%
ACAGGATATTGCACACAAAA+TGG + chr5.2:25673767-25673786 MS.gene68324:CDS 35.0%
ACTGTAGTTGTTGAGTGTAT+TGG - chr5.2:25674543-25674562 None:intergenic 35.0%
ACTTCTTACCTTTATATGGC+GGG + chr5.2:25674099-25674118 MS.gene68324:CDS 35.0%
AGTTGAAAAGGGAAATCTCA+AGG - chr5.2:25673019-25673038 None:intergenic 35.0%
ATACATTTATCCTGAGCTTC+AGG - chr5.2:25673472-25673491 None:intergenic 35.0%
ATATCCCGTGTTCAAATACA+TGG + chr5.2:25673546-25673565 MS.gene68324:CDS 35.0%
ATTTGACAACACTAAAGGCA+GGG - chr5.2:25672862-25672881 None:intergenic 35.0%
GATAACTTTGTGGTTATGGA+TGG + chr5.2:25674013-25674032 MS.gene68324:CDS 35.0%
GCAAAAAAAGAACCCAACAA+GGG - chr5.2:25673623-25673642 None:intergenic 35.0%
GTAATTTCCTGTCAGTATTG+TGG - chr5.2:25673354-25673373 None:intergenic 35.0%
TAAAACGACCTACATTTGGA+GGG - chr5.2:25673155-25673174 None:intergenic 35.0%
TACATTTATCCTGAGCTTCA+GGG - chr5.2:25673471-25673490 None:intergenic 35.0%
TAGCACTGTTCTTAGAACAA+TGG - chr5.2:25672984-25673003 None:intergenic 35.0%
TAGCTGATATTAAGCAAAGG+TGG - chr5.2:25674141-25674160 None:intergenic 35.0%
TATCCCGTGTTCAAATACAT+GGG + chr5.2:25673547-25673566 MS.gene68324:CDS 35.0%
TATTCCGAAGTAAGTCCAAA+TGG - chr5.2:25673921-25673940 None:intergenic 35.0%
TATTTGACAACACTAAAGGC+AGG - chr5.2:25672863-25672882 None:intergenic 35.0%
TGCAAAAAAAGAACCCAACA+AGG - chr5.2:25673624-25673643 None:intergenic 35.0%
! AGGATATTTGGAACTGATGT+AGG + chr5.2:25674699-25674718 MS.gene68324:intron 35.0%
! AGGGTTTTACTGTATTGTGA+AGG + chr5.2:25673886-25673905 MS.gene68324:intron 35.0%
! GAATGTTGATGAATAGTTCC+CGG - chr5.2:25674267-25674286 None:intergenic 35.0%
! TATCTGGACTTCCTTTTGTA+TGG + chr5.2:25672601-25672620 MS.gene68324:CDS 35.0%
! TCCTAGAATGTGTCTTTTGT+TGG - chr5.2:25674507-25674526 None:intergenic 35.0%
! TTGATTGTCAGCCTTTTAGT+TGG + chr5.2:25673112-25673131 MS.gene68324:intron 35.0%
!! ACATAGGTGTTGATACTGAT+AGG + chr5.2:25673065-25673084 MS.gene68324:intron 35.0%
!! TCCTTTTGTATGGATTGAGA+GGG + chr5.2:25672611-25672630 MS.gene68324:CDS 35.0%
!! TTATTTTTCGCCTGCATGAA+AGG - chr5.2:25673445-25673464 None:intergenic 35.0%
!! TTCCTTTTGTATGGATTGAG+AGG + chr5.2:25672610-25672629 MS.gene68324:CDS 35.0%
!!! GAGAGTGTTTTCTTCTTATC+TGG + chr5.2:25672585-25672604 MS.gene68324:CDS 35.0%
AAATAATAGCCCTGAAGCTC+AGG + chr5.2:25673459-25673478 MS.gene68324:CDS 40.0%
ACGAAGGAAAGACACATTCA+GGG - chr5.2:25674400-25674419 None:intergenic 40.0%
ACTAATGCTTCCTTTCATGC+AGG + chr5.2:25673432-25673451 MS.gene68324:intron 40.0%
ATATCAGCCATGGCAAATTG+TGG + chr5.2:25672880-25672899 MS.gene68324:intron 40.0%
ATGCCCATGTATTTGAACAC+GGG - chr5.2:25673553-25673572 None:intergenic 40.0%
CAACATTCACTTACATGGAC+CGG + chr5.2:25674279-25674298 MS.gene68324:CDS 40.0%
CAATACTTCCCTCCAAATGT+AGG + chr5.2:25673144-25673163 MS.gene68324:CDS 40.0%
CACCGAAATGATATAGCAAC+AGG - chr5.2:25673501-25673520 None:intergenic 40.0%
CATGCCCATGTATTTGAACA+CGG - chr5.2:25673554-25673573 None:intergenic 40.0%
CGCCTGTTGCTATATCATTT+CGG + chr5.2:25673496-25673515 MS.gene68324:CDS 40.0%
CTAAAACGACCTACATTTGG+AGG - chr5.2:25673156-25673175 None:intergenic 40.0%
CTGATATTAAGCAAAGGTGG+AGG - chr5.2:25674138-25674157 None:intergenic 40.0%
CTGTCAGTATTGTGGGAATT+CGG - chr5.2:25673346-25673365 None:intergenic 40.0%
GACACCATTTGGACTTACTT+CGG + chr5.2:25673914-25673933 MS.gene68324:CDS 40.0%
GCCAACAAAAGACACATTCT+AGG + chr5.2:25674503-25674522 MS.gene68324:intron 40.0%
GTGTTGTCAAATATCAGCCA+TGG + chr5.2:25672870-25672889 MS.gene68324:intron 40.0%
GTTGAGCAAGTACAAGATTC+AGG + chr5.2:25672641-25672660 MS.gene68324:intron 40.0%
TATGTCAGAGCGAACATTGT+TGG - chr5.2:25672787-25672806 None:intergenic 40.0%
TGTGTGAAAGGCCAACTAAA+AGG - chr5.2:25673126-25673145 None:intergenic 40.0%
! CTGCCGAAATACTTTTCCTT+GGG + chr5.2:25673949-25673968 MS.gene68324:CDS 40.0%
! GTCTTTTGTTGGCGATTTCA+TGG - chr5.2:25674496-25674515 None:intergenic 40.0%
! GTGGATTTTATGGCAGATTG+TGG + chr5.2:25672905-25672924 MS.gene68324:intron 40.0%
! TTATATTCTATTGGGGGCAC+AGG + chr5.2:25673749-25673768 MS.gene68324:CDS 40.0%
!! CGTTTGTTGTACACTAAGTC+CGG + chr5.2:25674245-25674264 MS.gene68324:CDS 40.0%
!! GTTTGTTGTACACTAAGTCC+GGG + chr5.2:25674246-25674265 MS.gene68324:CDS 40.0%
AAGAAGTTTGAGGGACTCCA+TGG - chr5.2:25674086-25674105 None:intergenic 45.0%
ACCTTCTTGAGTCCCTTGTT+GGG + chr5.2:25673608-25673627 MS.gene68324:intron 45.0%
AGAAGTTTGAGGGACTCCAT+GGG - chr5.2:25674085-25674104 None:intergenic 45.0%
ATTGCGGTTACCATTTCCCA+TGG + chr5.2:25674066-25674085 MS.gene68324:CDS 45.0%
ATTGGGTCCCTAAATGTGTG+AGG - chr5.2:25674573-25674592 None:intergenic 45.0%
CAACAATGGTTGCAAACCCA+AGG - chr5.2:25673968-25673987 None:intergenic 45.0%
CACGAAGGAAAGACACATTC+AGG - chr5.2:25674401-25674420 None:intergenic 45.0%
CAGAGCGAACATTGTTGGAA+CGG - chr5.2:25672782-25672801 None:intergenic 45.0%
CGAATTCCCACAATACTGAC+AGG + chr5.2:25673344-25673363 MS.gene68324:intron 45.0%
CTTGGGTTTGCAACCATTGT+TGG + chr5.2:25673966-25673985 MS.gene68324:CDS 45.0%
GACACCACCTCACACATTTA+GGG + chr5.2:25674563-25674582 MS.gene68324:intron 45.0%
GAGAGTCTTAGAGACAGTTG+AGG + chr5.2:25674040-25674059 MS.gene68324:CDS 45.0%
GCACAAGCCTCAAAATGAGA+AGG - chr5.2:25673298-25673317 None:intergenic 45.0%
GCCCTCTCAATCCATACAAA+AGG - chr5.2:25672615-25672634 None:intergenic 45.0%
GTGTCTTTCCTTCGTGAGTT+TGG + chr5.2:25674405-25674424 MS.gene68324:intron 45.0%
TCCACAACCACAATTTGCCA+TGG - chr5.2:25672890-25672909 None:intergenic 45.0%
TGACACCACCTCACACATTT+AGG + chr5.2:25674562-25674581 MS.gene68324:intron 45.0%
TGATGCGGCTGCAATATTTG+TGG + chr5.2:25673194-25673213 MS.gene68324:CDS 45.0%
TTCAAGGACCAAACTCACGA+AGG - chr5.2:25674416-25674435 None:intergenic 45.0%
! CCTGCCGAAATACTTTTCCT+TGG + chr5.2:25673948-25673967 MS.gene68324:CDS 45.0%
! TGAAGGTATGCGACACCATT+TGG + chr5.2:25673903-25673922 MS.gene68324:intron 45.0%
! TTTGTATGGATTGAGAGGGC+AGG + chr5.2:25672615-25672634 MS.gene68324:CDS 45.0%
!! AGAAGGTGAAAGTACCAGGA+TGG - chr5.2:25673595-25673614 None:intergenic 45.0%
!! CCAAGGAAAAGTATTTCGGC+AGG - chr5.2:25673951-25673970 None:intergenic 45.0%
!! CTCAAGAAGGTGAAAGTACC+AGG - chr5.2:25673599-25673618 None:intergenic 45.0%
!! GGAGGGAAGTATTGTGTGAA+AGG - chr5.2:25673138-25673157 None:intergenic 45.0%
!! GTGAAAGTACCAGGATGGTA+GGG - chr5.2:25673590-25673609 None:intergenic 45.0%
!! TGAAAGTACCAGGATGGTAG+GGG - chr5.2:25673589-25673608 None:intergenic 45.0%
AACCACAGCAACATGCAGTG+TGG - chr5.2:25673232-25673251 None:intergenic 50.0%
ACCCAACAAGGGACTCAAGA+AGG - chr5.2:25673612-25673631 None:intergenic 50.0%
AGTGATGGCAGGACCAACAA+TGG - chr5.2:25673982-25674001 None:intergenic 50.0%
ATCCACACTGCATGTTGCTG+TGG + chr5.2:25673227-25673246 MS.gene68324:CDS 50.0%
ATTGTTGGTCCTGCCATCAC+TGG + chr5.2:25673981-25674000 MS.gene68324:CDS 50.0%
CAACACTAAAGGCAGGGTCA+AGG - chr5.2:25672856-25672875 None:intergenic 50.0%
CACCTTCTTGAGTCCCTTGT+TGG + chr5.2:25673607-25673626 MS.gene68324:intron 50.0%
CCAGTTCACGTCACATGATG+CGG + chr5.2:25673179-25673198 MS.gene68324:CDS 50.0%
CTCACTCGTGATGTACACTG+TGG - chr5.2:25673807-25673826 None:intergenic 50.0%
GAGACAGTTGAGGCACATTG+CGG + chr5.2:25674050-25674069 MS.gene68324:CDS 50.0%
GAGACTGACCCGCCATATAA+AGG - chr5.2:25674110-25674129 None:intergenic 50.0%
GCCATGGCAAATTGTGGTTG+TGG + chr5.2:25672886-25672905 MS.gene68324:intron 50.0%
GTAATCTTCCCCTACCATCC+TGG + chr5.2:25673578-25673597 MS.gene68324:CDS 50.0%
TTGAGGGACTCCATGGGAAA+TGG - chr5.2:25674079-25674098 None:intergenic 50.0%
TTGTTGGTCCTGCCATCACT+GGG + chr5.2:25673982-25674001 MS.gene68324:CDS 50.0%
! GGCCCCACTTGATAACTTTG+TGG + chr5.2:25674003-25674022 MS.gene68324:CDS 50.0%
! TATGGATTGAGAGGGCAGGT+TGG + chr5.2:25672619-25672638 MS.gene68324:intron 50.0%
!! GGTGAAAGTACCAGGATGGT+AGG - chr5.2:25673591-25673610 None:intergenic 50.0%
CCGCATCATGTGACGTGAAC+TGG - chr5.2:25673182-25673201 None:intergenic 55.0%
GGGTCCCTAAATGTGTGAGG+TGG - chr5.2:25674570-25674589 None:intergenic 55.0%
TATCAAGTGGGGCCCAGTGA+TGG - chr5.2:25673997-25674016 None:intergenic 55.0%
AAGTGGGGCCCAGTGATGGC+AGG - chr5.2:25673993-25674012 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 25672583 25674788 25672583 ID=MS.gene68324
chr5.2 mRNA 25672583 25674788 25672583 ID=MS.gene68324.t1;Parent=MS.gene68324
chr5.2 exon 25672583 25672636 25672583 ID=MS.gene68324.t1.exon1;Parent=MS.gene68324.t1
chr5.2 CDS 25672583 25672636 25672583 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25673123 25673248 25673123 ID=MS.gene68324.t1.exon2;Parent=MS.gene68324.t1
chr5.2 CDS 25673123 25673248 25673123 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25673454 25673599 25673454 ID=MS.gene68324.t1.exon3;Parent=MS.gene68324.t1
chr5.2 CDS 25673454 25673599 25673454 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25673702 25673821 25673702 ID=MS.gene68324.t1.exon4;Parent=MS.gene68324.t1
chr5.2 CDS 25673702 25673821 25673702 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25673908 25674120 25673908 ID=MS.gene68324.t1.exon5;Parent=MS.gene68324.t1
chr5.2 CDS 25673908 25674120 25673908 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25674220 25674300 25674220 ID=MS.gene68324.t1.exon6;Parent=MS.gene68324.t1
chr5.2 CDS 25674220 25674300 25674220 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
chr5.2 exon 25674701 25674788 25674701 ID=MS.gene68324.t1.exon7;Parent=MS.gene68324.t1
chr5.2 CDS 25674701 25674788 25674701 ID=cds.MS.gene68324.t1;Parent=MS.gene68324.t1
Gene Sequence

>MS.gene68324

ATGAGAGTGTTTTCTTCTTATCTGGACTTCCTTTTGTATGGATTGAGAGGGCAGGTTGGTTGAGCAAGTACAAGATTCAGGTCTGCAGTTGACAGAATTTGTTCTTTATCCTTTATGTTGTAGATTCTTCCTTATGTTCCTACTGTTATGAATTACATCTCTTGTTGTATTTGGAAGTAAATCTTTCATAACTAAACCGTTCCAACAATGTTCGCTCTGACATAATATGCACATTTGACAGCGCACACATTGTTATTCATTTCAATCATTCCTTGACCCTGCCTTTAGTGTTGTCAAATATCAGCCATGGCAAATTGTGGTTGTGGATTTTATGGCAGATTGTGGTTGCAGATTTTTTAACAATCGCTAAATGCTTAATCAATAACACTGTCTGCTTTCCATTGTTCTAAGAACAGTGCTATAAGTGAAAGTTCCTTGAGATTTCCCTTTTCAACTTCTTCCTTATCTGTATGTATTACACAACATAGGTGTTGATACTGATAGGTTGTTCTAATTATTACTTTTGGTGTTGATTGTCAGCCTTTTAGTTGGCCTTTCACACAATACTTCCCTCCAAATGTAGGTCGTTTTAGTTTCCAGTTCACGTCACATGATGCGGCTGCAATATTTGTGGTTGCAACTGAATCCACACTGCATGTTGCTGTGGTTTAGTTTGAATAACTTATTGAGAGTGTTCAATTAATTTTTTTTTCCTTCTCATTTTGAGGCTTGTGCATGCTATATTTGATTATTGTATTAACCGAATTCCCACAATACTGACAGGAAATTACAATTGTTTTCCTCTTTCACATGTAAATACATAGTTCTACATATAAATCTATTGGTGTTACTAATGCTTCCTTTCATGCAGGCGAAAAATAATAGCCCTGAAGCTCAGGATAAATGTATTGTTCGCCTGTTGCTATATCATTTCGGTGTCAATCTACCTGTTATGATTTTTTCATATCCCGTGTTCAAATACATGGGCATGCAGAGTAATCTTCCCCTACCATCCTGGTACTTTCACCTTCTTGAGTCCCTTGTTGGGTTCTTTTTTTGCAAATATTTGGAATGCGTTTGTATTTAGATTACTTAACAAGATGAAATTTGGTTTTGCAGGAACATAATTCTAACTCAAATAATGTTTTACTTCATTTTGGAGGACTTTATATTCTATTGGGGGCACAGGATATTGCACACAAAATGGTTATACAAGCATGTCCACAGTGTACATCACGAGTGAGTGTTGTTACATGTGTTCTGCATTTTTGGCTTGTTATATTGATCATCTATGAACTTCTATAGGGTTTTACTGTATTGTGAAGGTATGCGACACCATTTGGACTTACTTCGGAATATGCTCATCCTGCCGAAATACTTTTCCTTGGGTTTGCAACCATTGTTGGTCCTGCCATCACTGGGCCCCACTTGATAACTTTGTGGTTATGGATGGTTGTGAGAGTCTTAGAGACAGTTGAGGCACATTGCGGTTACCATTTCCCATGGAGTCCCTCAAACTTCTTACCTTTATATGGCGGGTCAGTCTCAAGTTCCTCCACCTTTGCTTAATATCAGCTAGTTGGTTCATTCTTCTGTTGTACTTCTTGCTGTTAAACTTTTTATCCTCAATTTTTCAGATCTGATTTCCACGATTATCATCATCGTTTGTTGTACACTAAGTCCGGGAACTATTCATCAACATTCACTTACATGGACCGGTAAGCATCAAACATATTTTCCCTTAAGGATACTTGTTACGTTTTTAGAGTAAAAGGTCATTTTAGTCCTAGAATGTATCAAAATTACAAAAACTTCCCTGAATGTGTCTTTCCTTCGTGAGTTTGGTCCTTGAATATATCTTTCATTAGTTAATTTGATCGTCGAATGTCTTTTCTATTAGACAGTTTAGTCCATGAAATCGCCAACAAAAGACACATTCTAGGATACTTTTGTAATTCCAATACACTCAACAACTACAGTGACACCACCTCACACATTTAGGGACCCAATTGACGATTTACTTCATTTTTTATGATGGCTTAACTTAATGAACATTTGTAATGCCTTGGAATTTGATGCTTTACTAGTTTTCTATACTGAATTTTTTCCATGTATTAGGATATTTGGAACTGATGTAGGATACAGAAAGTTAAAAGCATTGAAGAGCAGAGAATTTGAAGACAGTTACGAGCAAAAGAAACAATAA
Protein sequence

>MS.gene68324.t1

MRVFSSYLDFLLYGLRGQPFSWPFTQYFPPNVGRFSFQFTSHDAAAIFVVATESTLHVAVAKNNSPEAQDKCIVRLLLYHFGVNLPVMIFSYPVFKYMGMQSNLPLPSWNIILTQIMFYFILEDFIFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVVRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRKLKALKSREFEDSYEQKKQ