Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052281.t1 | XP_013456565.1 | 100 | 272 | 0 | 0 | 1 | 272 | 1 | 272 | 2.60E-148 | 534.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052281.t1 | Q09473 | 33.2 | 226 | 132 | 5 | 47 | 268 | 2 | 212 | 2.5e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052281.t1 | I3SDS4 | 100.0 | 272 | 0 | 0 | 1 | 272 | 1 | 272 | 1.9e-148 | 534.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052281 | MS.gene22453 | 0.811126 | 8.18E-51 | -1.69E-46 |
| MS.gene052281 | MS.gene31726 | 0.822503 | 2.29E-53 | -1.69E-46 |
| MS.gene052281 | MS.gene32423 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene052281 | MS.gene53717 | 0.80392 | 2.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene003399 | MS.gene052281 | PPI |
| MS.gene052281 | MS.gene28949 | PPI |
| MS.gene052281 | MS.gene27841 | PPI |
| MS.gene023608 | MS.gene052281 | PPI |
| MS.gene052281 | MS.gene53264 | PPI |
| MS.gene052281 | MS.gene53261 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052281.t1 | MTR_4g075550 | 100.000 | 272 | 0 | 0 | 1 | 272 | 1 | 272 | 0.0 | 555 |
| MS.gene052281.t1 | MTR_3g113920 | 83.088 | 272 | 46 | 0 | 1 | 272 | 1 | 272 | 1.49e-172 | 477 |
| MS.gene052281.t1 | MTR_8g012595 | 49.077 | 271 | 135 | 3 | 1 | 268 | 1 | 271 | 1.35e-84 | 254 |
| MS.gene052281.t1 | MTR_8g012605 | 50.206 | 243 | 119 | 2 | 1 | 241 | 1 | 243 | 2.88e-83 | 251 |
| MS.gene052281.t1 | MTR_8g012615 | 52.821 | 195 | 91 | 1 | 75 | 268 | 9 | 203 | 3.37e-71 | 218 |
| MS.gene052281.t1 | MTR_8g012600 | 41.007 | 139 | 82 | 0 | 4 | 142 | 5 | 143 | 2.30e-31 | 118 |
| MS.gene052281.t1 | MTR_7g056337 | 34.061 | 229 | 130 | 8 | 47 | 269 | 2 | 215 | 5.47e-26 | 102 |
| MS.gene052281.t1 | MTR_8g087430 | 35.965 | 228 | 127 | 9 | 47 | 269 | 2 | 215 | 1.85e-25 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052281.t1 | AT2G21190 | 89.552 | 268 | 28 | 0 | 5 | 272 | 2 | 269 | 0.0 | 498 |
| MS.gene052281.t1 | AT4G38790 | 88.806 | 268 | 30 | 0 | 5 | 272 | 6 | 273 | 7.85e-179 | 493 |
| MS.gene052281.t1 | AT1G75760 | 85.662 | 272 | 39 | 0 | 1 | 272 | 1 | 272 | 7.41e-176 | 486 |
| MS.gene052281.t1 | AT1G19970 | 80.147 | 272 | 54 | 0 | 1 | 272 | 1 | 272 | 2.27e-169 | 469 |
| MS.gene052281.t1 | AT1G75760 | 85.547 | 256 | 37 | 0 | 1 | 256 | 1 | 256 | 6.51e-165 | 457 |
| MS.gene052281.t1 | AT1G75760 | 85.185 | 216 | 32 | 0 | 1 | 216 | 1 | 216 | 5.56e-137 | 385 |
| MS.gene052281.t1 | AT1G19970 | 75.983 | 229 | 51 | 1 | 1 | 229 | 1 | 225 | 5.22e-132 | 373 |
| MS.gene052281.t1 | AT3G25160 | 61.194 | 268 | 103 | 1 | 2 | 268 | 4 | 271 | 2.00e-121 | 348 |
| MS.gene052281.t1 | AT3G25040 | 34.934 | 229 | 128 | 9 | 47 | 269 | 2 | 215 | 2.61e-29 | 110 |
| MS.gene052281.t1 | AT1G29330 | 35.371 | 229 | 127 | 10 | 47 | 269 | 2 | 215 | 3.25e-28 | 107 |
| MS.gene052281.t1 | AT1G29330 | 35.172 | 145 | 81 | 5 | 125 | 269 | 22 | 153 | 8.89e-18 | 78.6 |
Find 68 sgRNAs with CRISPR-Local
Find 194 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCTTGGGGTATGGTTTA+TGG | 0.172228 | 4.2:-33350967 | MS.gene052281:CDS |
| CTGTGCTCATTGGGTTCTTC+AGG | 0.282833 | 4.2:-33351528 | MS.gene052281:intron |
| CATTCACACCATACTTGATT+TGG | 0.298660 | 4.2:-33352712 | MS.gene052281:CDS |
| CTGGGCATTCTGTGTATATC+TGG | 0.298963 | 4.2:-33351754 | MS.gene052281:CDS |
| AAAATATAGCTGTCAATTCC+TGG | 0.317259 | 4.2:+33352775 | None:intergenic |
| GGACATTTGTTGGTTGCCTT+GGG | 0.362241 | 4.2:-33350980 | MS.gene052281:CDS |
| GATTCTTAAGCTGTGCTCAT+TGG | 0.373170 | 4.2:-33351538 | MS.gene052281:CDS |
| TGGACATTTGTTGGTTGCCT+TGG | 0.385662 | 4.2:-33350981 | MS.gene052281:CDS |
| ATTCTTAAGCTGTGCTCATT+GGG | 0.386910 | 4.2:-33351537 | MS.gene052281:CDS |
| GCGAGAAAAGCCTTGACCTT+TGG | 0.406284 | 4.2:+33353396 | None:intergenic |
| CTTCTCATCATATATTGAAC+AGG | 0.417454 | 4.2:-33351782 | MS.gene052281:CDS |
| GAAAGTCGCCAAATCAAGTA+TGG | 0.428500 | 4.2:+33352704 | None:intergenic |
| TTGTTGGTTGCCTTGGGGTA+TGG | 0.433590 | 4.2:-33350974 | MS.gene052281:CDS |
| GCTGAAGCTGTTCACTCGCT+TGG | 0.434191 | 4.2:-33353300 | MS.gene052281:CDS |
| AGATACCCGTGGACATTTGT+TGG | 0.440531 | 4.2:-33350990 | MS.gene052281:CDS |
| AATCTGCTAAGATGAATGTT+TGG | 0.442427 | 4.2:+33350915 | None:intergenic |
| CTTGTTCTACGCCTTCCCTC+TGG | 0.447485 | 4.2:-33350738 | MS.gene052281:CDS |
| AATATATGATGAGAAGTTGA+TGG | 0.451706 | 4.2:+33351789 | None:intergenic |
| CGTGATCATGAACGATAAAT+CGG | 0.455398 | 4.2:+33353340 | None:intergenic |
| AGAAAAGCCTTGACCTTTGG+TGG | 0.458499 | 4.2:+33353399 | None:intergenic |
| GCGTGGATCGATGTTCTTTG+AGG | 0.459043 | 4.2:+33353447 | None:intergenic |
| TCTCGCCGTCGTTTCCGGTA+TGG | 0.465041 | 4.2:-33353379 | MS.gene052281:CDS |
| ACGCCATCTCCACATGGGTC+AGG | 0.477787 | 4.2:-33353428 | MS.gene052281:CDS |
| TGTTCTGCATGACCCGCAGT+TGG | 0.489763 | 4.2:+33351712 | None:intergenic |
| GTTCTGCATGACCCGCAGTT+GGG | 0.500169 | 4.2:+33351713 | None:intergenic |
| GAGAACAAGAGCGGCCATAC+CGG | 0.503947 | 4.2:+33353365 | None:intergenic |
| ATGAAGGAGAAGACTTGTGC+TGG | 0.507531 | 4.2:-33353252 | MS.gene052281:intron |
| TAAATTGAAGGCGAGTTACA+TGG | 0.511519 | 4.2:-33352646 | MS.gene052281:CDS |
| GACTTTCGTGACCACGGTGT+GGG | 0.516892 | 4.2:-33352688 | MS.gene052281:CDS |
| TCGCCTGACCCATGTGGAGA+TGG | 0.520457 | 4.2:+33353425 | None:intergenic |
| GTATGGTTTATGGCCGTCGA+TGG | 0.522435 | 4.2:-33350957 | MS.gene052281:CDS |
| TAACTTTGCAATTTACTATG+TGG | 0.522735 | 4.2:-33352613 | MS.gene052281:intron |
| TCTGTTACTATTATGTCAAG+AGG | 0.527285 | 4.2:-33350892 | MS.gene052281:intron |
| AAATATAGCTGTCAATTCCT+GGG | 0.533077 | 4.2:+33352776 | None:intergenic |
| CGATCCACGCCATCTCCACA+TGG | 0.539860 | 4.2:-33353434 | MS.gene052281:CDS |
| CGACTTTCGTGACCACGGTG+TGG | 0.547169 | 4.2:-33352689 | MS.gene052281:CDS |
| GGCGAGTTACATGGAGGAGA+AGG | 0.550641 | 4.2:-33352637 | MS.gene052281:CDS |
| AATACGTAATGAGCAGTGAA+TGG | 0.555239 | 4.2:+33351578 | None:intergenic |
| TTCAGTTCTACCCCAACTGC+GGG | 0.557419 | 4.2:-33351724 | MS.gene052281:CDS |
| TTCTGCATGACCCGCAGTTG+GGG | 0.562015 | 4.2:+33351714 | None:intergenic |
| AGGATTATCACTCAAATCCC+AGG | 0.579664 | 4.2:-33352793 | MS.gene052281:CDS |
| AGCAAGGCTAACACGGCACA+TGG | 0.585861 | 4.2:+33351819 | None:intergenic |
| AGCGGCCATACCGGAAACGA+CGG | 0.593225 | 4.2:+33353374 | None:intergenic |
| TTTCAGTTCTACCCCAACTG+CGG | 0.594339 | 4.2:-33351725 | MS.gene052281:CDS |
| GCTCATTACGTATTTGCACT+CGG | 0.595466 | 4.2:-33351569 | MS.gene052281:CDS |
| ATGAATGAGCAAGGCTAACA+CGG | 0.596304 | 4.2:+33351812 | None:intergenic |
| TCTTATCTATAAACTCATGA+AGG | 0.599329 | 4.2:-33353268 | MS.gene052281:CDS |
| TGCAATCACACCACTCCAGA+GGG | 0.600263 | 4.2:+33350723 | None:intergenic |
| GGCAACCAACAAATGTCCAC+GGG | 0.610059 | 4.2:+33350985 | None:intergenic |
| TGACCCATGTGGAGATGGCG+TGG | 0.610245 | 4.2:+33353430 | None:intergenic |
| AGGCAACCAACAAATGTCCA+CGG | 0.615244 | 4.2:+33350984 | None:intergenic |
| GCGACAACCACCAAAGGTCA+AGG | 0.619702 | 4.2:-33353406 | MS.gene052281:CDS |
| TCTACGCCTTCCCTCTGGAG+TGG | 0.628133 | 4.2:-33350733 | MS.gene052281:CDS |
| GGTCAGGCGACAACCACCAA+AGG | 0.628509 | 4.2:-33353412 | MS.gene052281:CDS |
| ATCACACCACTCCAGAGGGA+AGG | 0.629698 | 4.2:+33350727 | None:intergenic |
| GACATTTGTTGGTTGCCTTG+GGG | 0.633865 | 4.2:-33350979 | MS.gene052281:CDS |
| AGTTGATGGATGAATGAGCA+AGG | 0.649423 | 4.2:+33351803 | None:intergenic |
| CTGCAATCACACCACTCCAG+AGG | 0.651756 | 4.2:+33350722 | None:intergenic |
| TTTGGCGACTTTCGTGACCA+CGG | 0.658314 | 4.2:-33352694 | MS.gene052281:CDS |
| CGGCCATAAACCATACCCCA+AGG | 0.668614 | 4.2:+33350964 | None:intergenic |
| GATCCACGCCATCTCCACAT+GGG | 0.685742 | 4.2:-33353433 | MS.gene052281:CDS |
| GATCATGAACGATAAATCGG+AGG | 0.692285 | 4.2:+33353343 | None:intergenic |
| TCTGAGATAAGAACCATCGA+CGG | 0.702119 | 4.2:+33350944 | None:intergenic |
| AAATCGGAGGAGAACAAGAG+CGG | 0.713382 | 4.2:+33353356 | None:intergenic |
| ATTGAAGGCGAGTTACATGG+AGG | 0.718937 | 4.2:-33352643 | MS.gene052281:CDS |
| TGGTTGTCGCCTGACCCATG+TGG | 0.729979 | 4.2:+33353419 | None:intergenic |
| GCGGGTCATGCAGAACACCA+AGG | 0.742034 | 4.2:-33351706 | MS.gene052281:intron |
| CATGTATATAACCCACACCG+TGG | 0.775290 | 4.2:+33352677 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATAGTAAAAACAAATT+AGG | + | chr4.2:33353326-33353345 | None:intergenic | 10.0% |
| !!! | AAAAAAGAATTGAATTTTTT+TGG | - | chr4.2:33350983-33351002 | MS.gene052281:CDS | 10.0% |
| !!! | ATATATTTTTCTTTTGAAAA+TGG | - | chr4.2:33352327-33352346 | MS.gene052281:intron | 10.0% |
| !! | AAAAAACATAAAACATTACA+TGG | - | chr4.2:33351136-33351155 | MS.gene052281:intron | 15.0% |
| !! | AAAAACATAAAACATTACAT+GGG | - | chr4.2:33351137-33351156 | MS.gene052281:intron | 15.0% |
| !! | AATAATATGATTATGAAATC+TGG | - | chr4.2:33351907-33351926 | MS.gene052281:intron | 15.0% |
| !! | ACACAAAAATATTATTGATA+AGG | + | chr4.2:33351636-33351655 | None:intergenic | 15.0% |
| !! | GCAATTAAATAAATATCATT+AGG | + | chr4.2:33353405-33353424 | None:intergenic | 15.0% |
| !! | TGATTGATAAAACATAAAAA+GGG | + | chr4.2:33351076-33351095 | None:intergenic | 15.0% |
| !! | TTGATTGATAAAACATAAAA+AGG | + | chr4.2:33351077-33351096 | None:intergenic | 15.0% |
| !!! | ATGAAAAAGATCAATTTTTT+GGG | - | chr4.2:33351162-33351181 | MS.gene052281:intron | 15.0% |
| !!! | TTTTATGTTTTATCAATCAA+AGG | - | chr4.2:33351077-33351096 | MS.gene052281:intron | 15.0% |
| !! | AAAATATATCAAGCTGATAA+TGG | + | chr4.2:33352316-33352335 | None:intergenic | 20.0% |
| !! | AATCATATTATTCATGCAAA+AGG | + | chr4.2:33351899-33351918 | None:intergenic | 20.0% |
| !! | AGAACAAATAGTTAAAGATT+CGG | + | chr4.2:33351830-33351849 | None:intergenic | 20.0% |
| !! | ATTGCTCTTAAAAAATTACA+TGG | - | chr4.2:33351221-33351240 | MS.gene052281:intron | 20.0% |
| !! | GATTGATAAAACATAAAAAG+GGG | + | chr4.2:33351075-33351094 | None:intergenic | 20.0% |
| !! | TAAATTATTCCTCTTTCTAA+AGG | - | chr4.2:33352255-33352274 | MS.gene052281:intron | 20.0% |
| !! | TTGCTCTTAAAAAATTACAT+GGG | - | chr4.2:33351222-33351241 | MS.gene052281:intron | 20.0% |
| !!! | AGGTTAGATTTTTTAATAAC+TGG | - | chr4.2:33351097-33351116 | MS.gene052281:intron | 20.0% |
| !!! | CAATAATATTTTTGTGTCAA+TGG | - | chr4.2:33351639-33351658 | MS.gene052281:intron | 20.0% |
| !!! | CATGAAAAAGATCAATTTTT+TGG | - | chr4.2:33351161-33351180 | MS.gene052281:intron | 20.0% |
| !!! | CATTTTTTCCTACTTTTTTA+TGG | - | chr4.2:33351667-33351686 | MS.gene052281:intron | 20.0% |
| !!! | TTATTTAATTGCAGTGTTTT+CGG | - | chr4.2:33353413-33353432 | MS.gene052281:CDS | 20.0% |
| !!! | TTGAATCTTTTTGTTTTCTT+GGG | - | chr4.2:33351020-33351039 | MS.gene052281:intron | 20.0% |
| !!! | TTTGAATCTTTTTGTTTTCT+TGG | - | chr4.2:33351019-33351038 | MS.gene052281:intron | 20.0% |
| ! | AAGGCAATGCTTATAAATTT+AGG | + | chr4.2:33351617-33351636 | None:intergenic | 25.0% |
| ! | AATATATGATGAGAAGTTGA+TGG | + | chr4.2:33352395-33352414 | None:intergenic | 25.0% |
| ! | ACAAAATGAAACAATTCAGA+AGG | + | chr4.2:33352566-33352585 | None:intergenic | 25.0% |
| ! | AGAAATAGTATGTTAGTAGA+AGG | + | chr4.2:33353158-33353177 | None:intergenic | 25.0% |
| ! | ATGCAAAAGGAAATTTGAAT+CGG | + | chr4.2:33351886-33351905 | None:intergenic | 25.0% |
| ! | CATCTTTACCATAAAAAAGT+AGG | + | chr4.2:33351678-33351697 | None:intergenic | 25.0% |
| ! | GAAATATCTTAAGTCACTAT+CGG | + | chr4.2:33353360-33353379 | None:intergenic | 25.0% |
| ! | GATGCAATCATATTTGAATA+CGG | - | chr4.2:33352173-33352192 | MS.gene052281:intron | 25.0% |
| ! | GTTAGTTATAAATATGCAAC+TGG | + | chr4.2:33352722-33352741 | None:intergenic | 25.0% |
| ! | TAACTTTGCAATTTACTATG+TGG | - | chr4.2:33351568-33351587 | MS.gene052281:CDS | 25.0% |
| ! | TAATTAGGAGATTACAGATT+CGG | + | chr4.2:33352984-33353003 | None:intergenic | 25.0% |
| ! | TATTCAAATATGATTGCATC+AGG | + | chr4.2:33352173-33352192 | None:intergenic | 25.0% |
| ! | TCTTATCTATAAACTCATGA+AGG | - | chr4.2:33350913-33350932 | MS.gene052281:CDS | 25.0% |
| !! | AGATTTTTATCCCTTTAGAT+TGG | - | chr4.2:33351197-33351216 | MS.gene052281:intron | 25.0% |
| !! | ATATATTGAACAGGATTTTC+TGG | - | chr4.2:33352408-33352427 | MS.gene052281:intron | 25.0% |
| !! | TATATTGAACAGGATTTTCT+GGG | - | chr4.2:33352409-33352428 | MS.gene052281:intron | 25.0% |
| !! | TTTAGGATGAAACAAACAAT+TGG | + | chr4.2:33351600-33351619 | None:intergenic | 25.0% |
| !!! | CATGATTCGTTTTAAATTGA+AGG | - | chr4.2:33351523-33351542 | MS.gene052281:intron | 25.0% |
| !!! | CTTTTTTGTGTAAAATCGAA+GGG | + | chr4.2:33350970-33350989 | None:intergenic | 25.0% |
| !!! | GTTAATTTGATTTGTGTTTG+TGG | - | chr4.2:33351263-33351282 | MS.gene052281:intron | 25.0% |
| !!! | GTTTGTTTTTGTTTTGATGA+TGG | - | chr4.2:33351349-33351368 | MS.gene052281:intron | 25.0% |
| !!! | TCTTTTTTGTGTAAAATCGA+AGG | + | chr4.2:33350971-33350990 | None:intergenic | 25.0% |
| !!! | TTGGCTTAATGTGTTATTTT+TGG | - | chr4.2:33351286-33351305 | MS.gene052281:intron | 25.0% |
| !!! | TTTTTTGTGTAAAATCGAAG+GGG | + | chr4.2:33350969-33350988 | None:intergenic | 25.0% |
| AAAATATAGCTGTCAATTCC+TGG | + | chr4.2:33351409-33351428 | None:intergenic | 30.0% | |
| AAATATAGCTGTCAATTCCT+GGG | + | chr4.2:33351408-33351427 | None:intergenic | 30.0% | |
| AATCTGCTAAGATGAATGTT+TGG | + | chr4.2:33353269-33353288 | None:intergenic | 30.0% | |
| AATTAGGAGATTACAGATTC+GGG | + | chr4.2:33352983-33353002 | None:intergenic | 30.0% | |
| ATACTATTCAGTATTTACCC+AGG | + | chr4.2:33351946-33351965 | None:intergenic | 30.0% | |
| ATGGTTATGAATGATGTAAC+AGG | - | chr4.2:33351368-33351387 | MS.gene052281:intron | 30.0% | |
| ATGTCCATCATCTAATGTAT+GGG | - | chr4.2:33352522-33352541 | MS.gene052281:intron | 30.0% | |
| CTTAAAAAATTACATGGGTC+AGG | - | chr4.2:33351227-33351246 | MS.gene052281:intron | 30.0% | |
| CTTCTCATCATATATTGAAC+AGG | - | chr4.2:33352399-33352418 | MS.gene052281:intron | 30.0% | |
| GGCGTTAAAAATTTATACCT+TGG | + | chr4.2:33352495-33352514 | None:intergenic | 30.0% | |
| TAACAAGCTATCATGCAAAA+TGG | - | chr4.2:33351978-33351997 | MS.gene052281:intron | 30.0% | |
| TATCTTTCACATTACAGACA+AGG | + | chr4.2:33352948-33352967 | None:intergenic | 30.0% | |
| TATGTCCATCATCTAATGTA+TGG | - | chr4.2:33352521-33352540 | MS.gene052281:intron | 30.0% | |
| TATTTACCCAGGTTATTCTA+TGG | + | chr4.2:33351935-33351954 | None:intergenic | 30.0% | |
| TCTGTTACTATTATGTCAAG+AGG | - | chr4.2:33353289-33353308 | MS.gene052281:CDS | 30.0% | |
| TGTTACAATGTTTGTGTCTT+TGG | - | chr4.2:33353022-33353041 | MS.gene052281:intron | 30.0% | |
| TTATTACTAACCTGCAACAT+TGG | - | chr4.2:33352006-33352025 | MS.gene052281:intron | 30.0% | |
| TTCTTGGGTCTTTGTTAATT+GGG | - | chr4.2:33351035-33351054 | MS.gene052281:intron | 30.0% | |
| TTTCTTGGGTCTTTGTTAAT+TGG | - | chr4.2:33351034-33351053 | MS.gene052281:intron | 30.0% | |
| ! | AAAATCAAGCCTTTAGAAAG+AGG | + | chr4.2:33352267-33352286 | None:intergenic | 30.0% |
| ! | AACATACTATTTCTTGCATC+AGG | - | chr4.2:33353164-33353183 | MS.gene052281:intron | 30.0% |
| ! | ACATCTTATTGATAGTCATC+TGG | - | chr4.2:33353059-33353078 | MS.gene052281:intron | 30.0% |
| ! | AGGTATAAATTTTTAACGCC+AGG | - | chr4.2:33352495-33352514 | MS.gene052281:intron | 30.0% |
| ! | CATCTTATTGATAGTCATCT+GGG | - | chr4.2:33353060-33353079 | MS.gene052281:intron | 30.0% |
| ! | TAAATATGCAACTGGATTAG+AGG | + | chr4.2:33352714-33352733 | None:intergenic | 30.0% |
| ! | TTTTTGTGTAAAATCGAAGG+GGG | + | chr4.2:33350968-33350987 | None:intergenic | 30.0% |
| !! | ATATATCAAGCTGATAATGG+TGG | + | chr4.2:33352313-33352332 | None:intergenic | 30.0% |
| !! | ATTTGATTTGTGTTTGTGGT+TGG | - | chr4.2:33351267-33351286 | MS.gene052281:intron | 30.0% |
| !! | TTTCTTTTGAAAATGGTTGC+AGG | - | chr4.2:33352334-33352353 | MS.gene052281:intron | 30.0% |
| !!! | ACAGCTAAGCCATTTTTTAA+AGG | - | chr4.2:33352836-33352855 | MS.gene052281:intron | 30.0% |
| !!! | CAGCTATATTTTTAGCTGTT+AGG | - | chr4.2:33351417-33351436 | MS.gene052281:intron | 30.0% |
| !!! | TGATTGCATCAGGTTTTATT+TGG | + | chr4.2:33352163-33352182 | None:intergenic | 30.0% |
| AAAAGAAGAGGGGGTTTAAA+AGG | + | chr4.2:33352203-33352222 | None:intergenic | 35.0% | |
| AAGAGCAATGCCAATCTAAA+GGG | + | chr4.2:33351210-33351229 | None:intergenic | 35.0% | |
| AATACGTAATGAGCAGTGAA+TGG | + | chr4.2:33352606-33352625 | None:intergenic | 35.0% | |
| AGATCCCATACATTAGATGA+TGG | + | chr4.2:33352529-33352548 | None:intergenic | 35.0% | |
| AGTACAGGACTAAAAGAAGA+GGG | + | chr4.2:33352214-33352233 | None:intergenic | 35.0% | |
| ATATCATTAGGACAACAGCA+CGG | + | chr4.2:33353393-33353412 | None:intergenic | 35.0% | |
| ATATTCCACTCCTTGATCAA+CGG | + | chr4.2:33352765-33352784 | None:intergenic | 35.0% | |
| ATGGGTCAGGAAAAATTACA+TGG | - | chr4.2:33351240-33351259 | MS.gene052281:intron | 35.0% | |
| ATTCTTAAGCTGTGCTCATT+GGG | - | chr4.2:33352644-33352663 | MS.gene052281:CDS | 35.0% | |
| CATTCACACCATACTTGATT+TGG | - | chr4.2:33351469-33351488 | MS.gene052281:intron | 35.0% | |
| CGTGATCATGAACGATAAAT+CGG | + | chr4.2:33350844-33350863 | None:intergenic | 35.0% | |
| GACACAAACATTGTAACAGA+TGG | + | chr4.2:33353020-33353039 | None:intergenic | 35.0% | |
| TAAATTGAAGGCGAGTTACA+TGG | - | chr4.2:33351535-33351554 | MS.gene052281:CDS | 35.0% | |
| TAAGAGCAATGCCAATCTAA+AGG | + | chr4.2:33351211-33351230 | None:intergenic | 35.0% | |
| TGGGTCAGGAAAAATTACAT+GGG | - | chr4.2:33351241-33351260 | MS.gene052281:intron | 35.0% | |
| ! | CACAGGTTGCCTTTAAAAAA+TGG | + | chr4.2:33352848-33352867 | None:intergenic | 35.0% |
| ! | TAAGAATATGCAGAGAGTAC+AGG | + | chr4.2:33352229-33352248 | None:intergenic | 35.0% |
| ! | TTTAATTGCAGTGTTTTCGG+AGG | - | chr4.2:33353416-33353435 | MS.gene052281:CDS | 35.0% |
| !! | TTATTTGGTGATGTGTCACT+TGG | + | chr4.2:33352148-33352167 | None:intergenic | 35.0% |
| !!! | GCTGTATTGTAGTTTTGTCA+TGG | - | chr4.2:33351439-33351458 | MS.gene052281:intron | 35.0% |
| AGACTACAACACATGCACAA+TGG | + | chr4.2:33352904-33352923 | None:intergenic | 40.0% | |
| AGGATTATCACTCAAATCCC+AGG | - | chr4.2:33351388-33351407 | MS.gene052281:intron | 40.0% | |
| AGGTTACAAAACCAGAGAGT+TGG | + | chr4.2:33353110-33353129 | None:intergenic | 40.0% | |
| ATATGCCTGCATACTAGAGT+AGG | - | chr4.2:33352062-33352081 | MS.gene052281:intron | 40.0% | |
| ATGAATGAGCAAGGCTAACA+CGG | + | chr4.2:33352372-33352391 | None:intergenic | 40.0% | |
| CAGCATGCACATGATTTACT+AGG | - | chr4.2:33352102-33352121 | MS.gene052281:intron | 40.0% | |
| CTGGATCCATAGAATAACCT+GGG | - | chr4.2:33351926-33351945 | MS.gene052281:intron | 40.0% | |
| GAAAGTCGCCAAATCAAGTA+TGG | + | chr4.2:33351480-33351499 | None:intergenic | 40.0% | |
| GACTACAACACATGCACAAT+GGG | + | chr4.2:33352903-33352922 | None:intergenic | 40.0% | |
| GAGTACAGGACTAAAAGAAG+AGG | + | chr4.2:33352215-33352234 | None:intergenic | 40.0% | |
| GATCATGAACGATAAATCGG+AGG | + | chr4.2:33350841-33350860 | None:intergenic | 40.0% | |
| GATTCTTAAGCTGTGCTCAT+TGG | - | chr4.2:33352643-33352662 | MS.gene052281:CDS | 40.0% | |
| GCTCATTACGTATTTGCACT+CGG | - | chr4.2:33352612-33352631 | MS.gene052281:intron | 40.0% | |
| GGAGTTGCTGTCATGTAATT+AGG | + | chr4.2:33352999-33353018 | None:intergenic | 40.0% | |
| GGGAAAGACGACTCAAAAAA+TGG | + | chr4.2:33352744-33352763 | None:intergenic | 40.0% | |
| GTACAGGACTAAAAGAAGAG+GGG | + | chr4.2:33352213-33352232 | None:intergenic | 40.0% | |
| TACAGGACTAAAAGAAGAGG+GGG | + | chr4.2:33352212-33352231 | None:intergenic | 40.0% | |
| TATGCCTGCATACTAGAGTA+GGG | - | chr4.2:33352063-33352082 | MS.gene052281:intron | 40.0% | |
| TATTCCACTCCTTGATCAAC+GGG | + | chr4.2:33352764-33352783 | None:intergenic | 40.0% | |
| TCTGGATCCATAGAATAACC+TGG | - | chr4.2:33351925-33351944 | MS.gene052281:intron | 40.0% | |
| TGGGTCTTTGTTAATTGGGT+AGG | - | chr4.2:33351039-33351058 | MS.gene052281:intron | 40.0% | |
| TGTATACTACCATTGCACAC+AGG | + | chr4.2:33351783-33351802 | None:intergenic | 40.0% | |
| TGTTAGTAGAAGGAGACATG+CGG | + | chr4.2:33353148-33353167 | None:intergenic | 40.0% | |
| ! | TCTGAGATAAGAACCATCGA+CGG | + | chr4.2:33353240-33353259 | None:intergenic | 40.0% |
| !! | AGTTGATGGATGAATGAGCA+AGG | + | chr4.2:33352381-33352400 | None:intergenic | 40.0% |
| !! | ATCAAGCTGATAATGGTGGA+AGG | + | chr4.2:33352309-33352328 | None:intergenic | 40.0% |
| !! | CTTGGTTCTATTGCAAGAAG+AGG | + | chr4.2:33352130-33352149 | None:intergenic | 40.0% |
| !! | TAGATGATGGACATAGATCC+TGG | + | chr4.2:33352516-33352535 | None:intergenic | 40.0% |
| !!! | CTCTGGTTTTGTAACCTTCT+TGG | - | chr4.2:33353113-33353132 | MS.gene052281:intron | 40.0% |
| AAATCGGAGGAGAACAAGAG+CGG | + | chr4.2:33350828-33350847 | None:intergenic | 45.0% | |
| AGAAAAGCCTTGACCTTTGG+TGG | + | chr4.2:33350785-33350804 | None:intergenic | 45.0% | |
| AGATACCCGTGGACATTTGT+TGG | - | chr4.2:33353191-33353210 | MS.gene052281:intron | 45.0% | |
| AGGCAACCAACAAATGTCCA+CGG | + | chr4.2:33353200-33353219 | None:intergenic | 45.0% | |
| ATAAGCGCAACCAATGTTGC+AGG | + | chr4.2:33352019-33352038 | None:intergenic | 45.0% | |
| ATGAAGGAGAAGACTTGTGC+TGG | - | chr4.2:33350929-33350948 | MS.gene052281:CDS | 45.0% | |
| ATTGAAGGCGAGTTACATGG+AGG | - | chr4.2:33351538-33351557 | MS.gene052281:CDS | 45.0% | |
| CATGTATATAACCCACACCG+TGG | + | chr4.2:33351507-33351526 | None:intergenic | 45.0% | |
| CTGGGCATTCTGTGTATATC+TGG | - | chr4.2:33352427-33352446 | MS.gene052281:intron | 45.0% | |
| GATATGTCACCTGTGTGCAA+TGG | - | chr4.2:33351771-33351790 | MS.gene052281:CDS | 45.0% | |
| TGCATACTAGAGTAGGGTCT+GGG | - | chr4.2:33352069-33352088 | MS.gene052281:intron | 45.0% | |
| TGCGGTTACAGAATCCAAGA+AGG | + | chr4.2:33353130-33353149 | None:intergenic | 45.0% | |
| TTGCCTTGGGGTATGGTTTA+TGG | - | chr4.2:33353214-33353233 | MS.gene052281:intron | 45.0% | |
| TTTCAGTTCTACCCCAACTG+CGG | - | chr4.2:33352456-33352475 | MS.gene052281:intron | 45.0% | |
| ! | AAAGCTCTTGCCATTTGGTG+AGG | - | chr4.2:33352872-33352891 | MS.gene052281:intron | 45.0% |
| ! | CAACACATGCACAATGGGTT+TGG | + | chr4.2:33352898-33352917 | None:intergenic | 45.0% |
| ! | GACATTTGTTGGTTGCCTTG+GGG | - | chr4.2:33353202-33353221 | MS.gene052281:intron | 45.0% |
| ! | GGACATTTGTTGGTTGCCTT+GGG | - | chr4.2:33353201-33353220 | MS.gene052281:intron | 45.0% |
| ! | TGGACATTTGTTGGTTGCCT+TGG | - | chr4.2:33353200-33353219 | MS.gene052281:intron | 45.0% |
| !! | GCTCTAAAGCTCTTGCCATT+TGG | - | chr4.2:33352867-33352886 | MS.gene052281:intron | 45.0% |
| !!! | CATCAGGTTTTAGATACCCG+TGG | - | chr4.2:33353180-33353199 | MS.gene052281:intron | 45.0% |
| !!! | CTGTTTTTGCACCAACTCTC+TGG | - | chr4.2:33353096-33353115 | MS.gene052281:intron | 45.0% |
| ATGGGTTTGGCCTCACCAAA+TGG | + | chr4.2:33352885-33352904 | None:intergenic | 50.0% | |
| CAGACCCTACTCTAGTATGC+AGG | + | chr4.2:33352070-33352089 | None:intergenic | 50.0% | |
| CTGCATACTAGAGTAGGGTC+TGG | - | chr4.2:33352068-33352087 | MS.gene052281:intron | 50.0% | |
| CTGTGCTCATTGGGTTCTTC+AGG | - | chr4.2:33352653-33352672 | MS.gene052281:CDS | 50.0% | |
| CTTTCCCGTTGATCAAGGAG+TGG | - | chr4.2:33352757-33352776 | MS.gene052281:CDS | 50.0% | |
| GCATACTAGAGTAGGGTCTG+GGG | - | chr4.2:33352070-33352089 | MS.gene052281:intron | 50.0% | |
| GCGAGAAAAGCCTTGACCTT+TGG | + | chr4.2:33350788-33350807 | None:intergenic | 50.0% | |
| GCGTGGATCGATGTTCTTTG+AGG | + | chr4.2:33350737-33350756 | None:intergenic | 50.0% | |
| GGCAACCAACAAATGTCCAC+GGG | + | chr4.2:33353199-33353218 | None:intergenic | 50.0% | |
| GTATGGTTTATGGCCGTCGA+TGG | - | chr4.2:33353224-33353243 | MS.gene052281:intron | 50.0% | |
| GTCGTCTTTCCCGTTGATCA+AGG | - | chr4.2:33352752-33352771 | MS.gene052281:CDS | 50.0% | |
| TAGGACAACAGCACGGAGTA+AGG | + | chr4.2:33353386-33353405 | None:intergenic | 50.0% | |
| TTCAGTTCTACCCCAACTGC+GGG | - | chr4.2:33352457-33352476 | MS.gene052281:intron | 50.0% | |
| TTTGGCGACTTTCGTGACCA+CGG | - | chr4.2:33351487-33351506 | MS.gene052281:intron | 50.0% | |
| ! | TTGTTGGTTGCCTTGGGGTA+TGG | - | chr4.2:33353207-33353226 | MS.gene052281:intron | 50.0% |
| !! | TGGCAAGAGCTTTAGAGCAC+AGG | + | chr4.2:33352865-33352884 | None:intergenic | 50.0% |
| ACAACAGCACGGAGTAAGGC+AGG | + | chr4.2:33353382-33353401 | None:intergenic | 55.0% | |
| AGCAAGGCTAACACGGCACA+TGG | + | chr4.2:33352365-33352384 | None:intergenic | 55.0% | |
| CGGCCATAAACCATACCCCA+AGG | + | chr4.2:33353220-33353239 | None:intergenic | 55.0% | |
| CTTGTTCTACGCCTTCCCTC+TGG | - | chr4.2:33353443-33353462 | MS.gene052281:CDS | 55.0% | |
| GACTTTCGTGACCACGGTGT+GGG | - | chr4.2:33351493-33351512 | MS.gene052281:intron | 55.0% | |
| GAGAACAAGAGCGGCCATAC+CGG | + | chr4.2:33350819-33350838 | None:intergenic | 55.0% | |
| GATCCACGCCATCTCCACAT+GGG | - | chr4.2:33350748-33350767 | MS.gene052281:CDS | 55.0% | |
| GCGACAACCACCAAAGGTCA+AGG | - | chr4.2:33350775-33350794 | MS.gene052281:intron | 55.0% | |
| GCTGAAGCTGTTCACTCGCT+TGG | - | chr4.2:33350881-33350900 | MS.gene052281:intron | 55.0% | |
| GGCGAGTTACATGGAGGAGA+AGG | - | chr4.2:33351544-33351563 | MS.gene052281:CDS | 55.0% | |
| GTTCTGCATGACCCGCAGTT+GGG | + | chr4.2:33352471-33352490 | None:intergenic | 55.0% | |
| TGTTCTGCATGACCCGCAGT+TGG | + | chr4.2:33352472-33352491 | None:intergenic | 55.0% | |
| TTCTGCATGACCCGCAGTTG+GGG | + | chr4.2:33352470-33352489 | None:intergenic | 55.0% | |
| ! | GCTTTTCTCGCCGTCGTTTC+CGG | - | chr4.2:33350797-33350816 | MS.gene052281:intron | 55.0% |
| ACGCCATCTCCACATGGGTC+AGG | - | chr4.2:33350753-33350772 | MS.gene052281:CDS | 60.0% | |
| AGCGGCCATACCGGAAACGA+CGG | + | chr4.2:33350810-33350829 | None:intergenic | 60.0% | |
| CGACTTTCGTGACCACGGTG+TGG | - | chr4.2:33351492-33351511 | MS.gene052281:intron | 60.0% | |
| CGATCCACGCCATCTCCACA+TGG | - | chr4.2:33350747-33350766 | MS.gene052281:CDS | 60.0% | |
| GCGGGTCATGCAGAACACCA+AGG | - | chr4.2:33352475-33352494 | MS.gene052281:intron | 60.0% | |
| GGTCAGGCGACAACCACCAA+AGG | - | chr4.2:33350769-33350788 | MS.gene052281:intron | 60.0% | |
| TCGCCTGACCCATGTGGAGA+TGG | + | chr4.2:33350759-33350778 | None:intergenic | 60.0% | |
| TCTCGCCGTCGTTTCCGGTA+TGG | - | chr4.2:33350802-33350821 | MS.gene052281:intron | 60.0% | |
| TGACCCATGTGGAGATGGCG+TGG | + | chr4.2:33350754-33350773 | None:intergenic | 60.0% | |
| TGGTTGTCGCCTGACCCATG+TGG | + | chr4.2:33350765-33350784 | None:intergenic | 60.0% | |
| ! | TCTACGCCTTCCCTCTGGAG+TGG | - | chr4.2:33353448-33353467 | MS.gene052281:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 33350728 | 33353475 | 33350728 | ID=MS.gene052281 |
| chr4.2 | mRNA | 33350728 | 33353475 | 33350728 | ID=MS.gene052281.t1;Parent=MS.gene052281 |
| chr4.2 | exon | 33353253 | 33353475 | 33353253 | ID=MS.gene052281.t1.exon1;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33353253 | 33353475 | 33353253 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| chr4.2 | exon | 33352614 | 33352813 | 33352614 | ID=MS.gene052281.t1.exon2;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33352614 | 33352813 | 33352614 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| chr4.2 | exon | 33351707 | 33351847 | 33351707 | ID=MS.gene052281.t1.exon3;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33351707 | 33351847 | 33351707 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| chr4.2 | exon | 33351529 | 33351609 | 33351529 | ID=MS.gene052281.t1.exon4;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33351529 | 33351609 | 33351529 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| chr4.2 | exon | 33350893 | 33351017 | 33350893 | ID=MS.gene052281.t1.exon5;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33350893 | 33351017 | 33350893 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| chr4.2 | exon | 33350728 | 33350776 | 33350728 | ID=MS.gene052281.t1.exon6;Parent=MS.gene052281.t1 |
| chr4.2 | CDS | 33350728 | 33350776 | 33350728 | ID=cds.MS.gene052281.t1;Parent=MS.gene052281.t1 |
| Gene Sequence |
| Protein sequence |
