AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025273

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025273.t1 MTR_2g042900 96.443 253 6 1 1 250 1 253 0.0 501
MS.gene025273.t1 MTR_2g042900 85.156 256 5 2 1 223 1 256 3.49e-154 429
MS.gene025273.t1 MTR_4g124960 44.961 258 77 10 1 248 1 203 1.44e-48 159
MS.gene025273.t1 MTR_4g124960 44.934 227 69 8 1 226 1 172 2.35e-42 142
MS.gene025273.t1 MTR_5g013515 45.513 156 47 6 99 242 3 132 4.92e-35 122
MS.gene025273.t1 MTR_5g013530 46.309 149 38 4 105 242 18 135 8.95e-34 119
MS.gene025273.t1 MTR_5g013530 44.512 164 43 5 96 242 25 157 1.26e-33 120
MS.gene025273.t1 MTR_5g013520 46.622 148 38 5 105 242 11 127 2.04e-33 118
MS.gene025273.t1 MTR_8g021380 31.441 229 121 6 12 233 11 210 9.45e-23 93.2
MS.gene025273.t1 MTR_8g021380 30.165 242 128 8 12 223 11 241 1.77e-15 74.3
MS.gene025273.t1 MTR_4g124950 55.072 69 25 3 69 137 1 63 6.41e-15 69.7
MS.gene025273.t1 MTR_2g044910 32.031 128 62 5 109 230 15 123 1.09e-13 67.4
MS.gene025273.t1 MTR_2g044910 32.031 128 62 5 109 230 58 166 1.60e-13 67.8
MS.gene025273.t1 MTR_1g031930 29.885 174 68 4 107 237 111 273 5.63e-12 64.7
MS.gene025273.t1 MTR_1g031930 29.885 174 68 4 107 237 172 334 2.57e-11 63.2
MS.gene025273.t1 MTR_4g124360 28.682 129 73 3 105 230 3 115 4.73e-11 59.7
MS.gene025273.t1 MTR_4g124360 28.682 129 73 3 105 230 41 153 6.40e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025273.t1 AT1G74950 37.143 280 101 9 2 242 3 246 2.32e-43 147
MS.gene025273.t1 AT1G19180 36.667 270 120 8 1 242 1 247 6.76e-41 141
MS.gene025273.t1 AT1G19180 36.667 270 120 8 1 242 1 247 6.76e-41 141
MS.gene025273.t1 AT1G74950 36.863 255 92 8 2 223 3 221 4.11e-37 130
MS.gene025273.t1 AT1G72450 32.759 232 120 10 17 234 4 213 7.91e-27 105
MS.gene025273.t1 AT1G17380 30.279 251 130 7 1 241 1 216 1.29e-24 99.4
MS.gene025273.t1 AT1G19180 41.096 146 56 6 106 242 57 181 3.17e-22 90.9
MS.gene025273.t1 AT1G72450 30.769 221 117 10 17 223 4 202 6.16e-22 90.5
MS.gene025273.t1 AT1G17380 29.493 217 115 6 17 223 10 198 4.08e-19 83.2
MS.gene025273.t1 AT1G17380 34.783 138 64 4 105 236 65 182 8.71e-14 69.3
MS.gene025273.t1 AT5G20900 31.967 122 68 2 109 230 57 163 3.31e-12 63.9
MS.gene025273.t1 AT3G17860 32.278 158 72 6 108 234 177 330 9.03e-11 61.6
MS.gene025273.t1 AT3G17860 32.278 158 72 6 108 234 144 297 9.26e-11 61.2
MS.gene025273.t1 AT3G17860 32.278 158 72 6 108 234 144 297 9.26e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGAGTCAATATATTA+AGG 0.231958 2.2:-44922372 MS.gene025273:CDS
GCCTTCATTTCCTCCTAATT+TGG 0.255924 2.2:-44920882 MS.gene025273:CDS
AGCCTCGCTTCATCGGTTTC+TGG 0.260318 2.2:-44920627 MS.gene025273:CDS
GCTGGCTTATTAACTTGATA+TGG 0.264533 2.2:+44920446 None:intergenic
GCCGGTGCCGGTGGCAGTAC+TGG 0.269465 2.2:+44920805 None:intergenic
ACCGAAGTTCTGACCAAATT+AGG 0.285058 2.2:+44920869 None:intergenic
TCGGAATATTCAGAAGTTTC+CGG 0.324209 2.2:-44922449 MS.gene025273:CDS
ATTGTCAATTGTGCAGCTTT+AGG 0.328266 2.2:+44921034 None:intergenic
CTTCGCCGGTGGTTTGTTGC+CGG 0.328284 2.2:+44922430 None:intergenic
ACAAAAGAACCTTACAACTA+TGG 0.341905 2.2:-44921651 MS.gene025273:CDS
CATGGTTGGAAACAAATTCA+TGG 0.354972 2.2:+44921688 None:intergenic
CATGAGCTCATGTGCTTTGT+CGG 0.371987 2.2:+44920970 None:intergenic
GAGTCAATATATTAAGGAAA+AGG 0.376605 2.2:-44922366 MS.gene025273:CDS
CCGGTGCCGGTGGCAGTACT+GGG 0.382396 2.2:+44920806 None:intergenic
TGCTTCAAAGATCTTTCTCT+TGG 0.389248 2.2:-44922341 MS.gene025273:CDS
AAGAAATTACCGCAAAGATT+AGG 0.403918 2.2:+44920749 None:intergenic
AGTCAATATATTAAGGAAAA+GGG 0.413916 2.2:-44922365 MS.gene025273:CDS
AGCTCATGTGCTTTGTCGGC+AGG 0.427800 2.2:+44920974 None:intergenic
ATGTTCACAGTAGGAACAAC+TGG 0.428017 2.2:+44920833 None:intergenic
AGCGAGGCTTTCCTCATGAT+TGG 0.432771 2.2:+44920641 None:intergenic
CATGAATTTGTTTCCAACCA+TGG 0.434083 2.2:-44921687 MS.gene025273:CDS
AATGATTTGCAGTGCTATCA+AGG 0.444008 2.2:-44921059 MS.gene025273:intron
CCTTCCAGACCTAATCTTTG+CGG 0.448657 2.2:-44920758 MS.gene025273:intron
CCCAGTACTGCCACCGGCAC+CGG 0.456188 2.2:-44920806 MS.gene025273:CDS
TTCATTAATCGAGCCGGTGC+CGG 0.460250 2.2:+44920793 None:intergenic
ATTACCGCAAAGATTAGGTC+TGG 0.470927 2.2:+44920754 None:intergenic
GAGTCCATGTCATGGCTTGT+GGG 0.483230 2.2:-44920422 MS.gene025273:CDS
TCCTAATTTGGTCAGAACTT+CGG 0.497883 2.2:-44920870 MS.gene025273:CDS
TGAGGAAAGCCTCGCTTCAT+CGG 0.506230 2.2:-44920634 MS.gene025273:CDS
TAGGTCTGGAAGGCACTTGA+AGG 0.506440 2.2:+44920768 None:intergenic
ACATGCAACACAGACCCTTC+TGG 0.507485 2.2:-44922314 MS.gene025273:intron
TCGGTTTCTGGAGAAGAGAA+AGG 0.512539 2.2:-44920615 MS.gene025273:CDS
CAAACCACCGGCGAAGTCAC+CGG 0.513459 2.2:-44922423 MS.gene025273:CDS
CGACAAAGCACATGAGCTCA+TGG 0.524937 2.2:-44920969 MS.gene025273:CDS
TGAGCAAATCCATAGTTGTA+AGG 0.532508 2.2:+44921642 None:intergenic
ATTAACTTGATATGGTGCAT+TGG 0.537946 2.2:+44920454 None:intergenic
TGAGTCCATGTCATGGCTTG+TGG 0.539136 2.2:-44920423 MS.gene025273:CDS
GAATTGTTCAAAGCAGCTTG+TGG 0.542652 2.2:+44921617 None:intergenic
TTCAACTATGTCTACCTCAT+CGG 0.558400 2.2:-44922468 None:intergenic
TGCACCCACAAGCCATGACA+TGG 0.562173 2.2:+44920418 None:intergenic
ACCAAATTAGGAGGAAATGA+AGG 0.565826 2.2:+44920881 None:intergenic
TTCTCCGGTGACTTCGCCGG+TGG 0.568647 2.2:+44922419 None:intergenic
AAGGTGTTCATTAATCGAGC+CGG 0.572295 2.2:+44920787 None:intergenic
AGCCATGACATGGACTCAGC+TGG 0.572954 2.2:+44920428 None:intergenic
AATTAGGAGGAAATGAAGGC+TGG 0.580229 2.2:+44920885 None:intergenic
ATTAATCGAGCCGGTGCCGG+TGG 0.588264 2.2:+44920796 None:intergenic
CCGCAAAGATTAGGTCTGGA+AGG 0.596635 2.2:+44920758 None:intergenic
ATCATCCCCAGTACTGCCAC+CGG 0.600357 2.2:-44920812 MS.gene025273:CDS
ATTAAGGTTGCGTACCAGAA+GGG 0.608421 2.2:+44922300 None:intergenic
CTCCAGAAACCGATGAAGCG+AGG 0.614975 2.2:+44920625 None:intergenic
TTCTGGAGAAGAGAAAGGAT+AGG 0.619484 2.2:-44920610 MS.gene025273:intron
GAAGTTCTGACCAAATTAGG+AGG 0.621959 2.2:+44920872 None:intergenic
ATGATTTGCAGTGCTATCAA+GGG 0.622638 2.2:-44921058 MS.gene025273:intron
AGTTTCCGGCAACAAACCAC+CGG 0.625545 2.2:-44922435 MS.gene025273:CDS
CTGGGGATGATGTTCACAGT+AGG 0.632088 2.2:+44920824 None:intergenic
CGGTGCCGGTGGCAGTACTG+GGG 0.633630 2.2:+44920807 None:intergenic
GTGCAGATCTGCCAATCATG+AGG 0.639785 2.2:-44920652 MS.gene025273:intron
AGCCAGCTGAGTCCATGTCA+TGG 0.640187 2.2:-44920430 MS.gene025273:CDS
CACAGTAGGAACAACTGGAG+CGG 0.676854 2.2:+44920838 None:intergenic
AACTTCTGAATATTCCGATG+AGG 0.677602 2.2:+44922454 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTATTTTTTTAAAATT+TGG - chr2.2:44920651-44920670 MS.gene025273:intron 0.0%
!! AAAAAAAAAAATAAACAACA+TGG + chr2.2:44920905-44920924 None:intergenic 10.0%
!! AAAAATTAATAATCAACTAA+TGG + chr2.2:44921745-44921764 None:intergenic 10.0%
!! AGTTAAAAAAATAAATTTGA+TGG - chr2.2:44921474-44921493 MS.gene025273:intron 10.0%
!!! ATTGTTATTATATACTAATT+AGG - chr2.2:44920993-44921012 MS.gene025273:CDS 10.0%
!! CATACAAGAATAATTTAAAA+TGG + chr2.2:44920861-44920880 None:intergenic 15.0%
!! TTTATTGAGTCAATATATTA+AGG - chr2.2:44920489-44920508 MS.gene025273:intron 15.0%
!!! AATCTTTGAAATTTTTTTGT+GGG - chr2.2:44920623-44920642 MS.gene025273:CDS 15.0%
!!! ATTTTTTTACAAACCTAATT+TGG + chr2.2:44921320-44921339 None:intergenic 15.0%
!!! TTATTTTTTCCTTTTTTGTA+AGG - chr2.2:44920805-44920824 MS.gene025273:CDS 15.0%
!!! TTTAAAATTTGGTATCTTAA+TGG - chr2.2:44920662-44920681 MS.gene025273:intron 15.0%
!!! TTTTTTTGTGTAATATTGTT+TGG - chr2.2:44921085-44921104 MS.gene025273:intron 15.0%
!! AAAAAATAAATTTGATGGAG+AGG - chr2.2:44921479-44921498 MS.gene025273:intron 20.0%
!! AGTCAATATATTAAGGAAAA+GGG - chr2.2:44920496-44920515 MS.gene025273:intron 20.0%
!! ATTTCATTTCATACCAAATT+AGG - chr2.2:44921304-44921323 MS.gene025273:intron 20.0%
!! GAAACAAAAAACGAAAAATT+CGG + chr2.2:44920591-44920610 None:intergenic 20.0%
!!! AATTTTTCGTTTTTTGTTTC+TGG - chr2.2:44920591-44920610 MS.gene025273:intron 20.0%
!!! ATTTTTCGTTTTTTGTTTCT+GGG - chr2.2:44920592-44920611 MS.gene025273:intron 20.0%
!!! CAATCTTTGAAATTTTTTTG+TGG - chr2.2:44920622-44920641 MS.gene025273:CDS 20.0%
!!! CAATTGACAATGTTTTATAA+TGG - chr2.2:44921839-44921858 MS.gene025273:intron 20.0%
!!! TTGTTTTTTTTTTCTTTGCA+GGG - chr2.2:44921119-44921138 MS.gene025273:intron 20.0%
!!! TTTGTTTTTTTTTTCTTTGC+AGG - chr2.2:44921118-44921137 MS.gene025273:intron 20.0%
! AATTTATGAGTGATGACAAA+TGG - chr2.2:44921341-44921360 MS.gene025273:intron 25.0%
! ATTATTCCTTTCTTCTTAGT+TGG - chr2.2:44920688-44920707 MS.gene025273:intron 25.0%
! ATTCTAGTACTTAAGAGTAA+CGG - chr2.2:44920725-44920744 MS.gene025273:intron 25.0%
! GAGTCAATATATTAAGGAAA+AGG - chr2.2:44920495-44920514 MS.gene025273:intron 25.0%
!! ATAGTTCTACCTTACAAAAA+AGG + chr2.2:44920817-44920836 None:intergenic 25.0%
AACCATTGACACGTAAAATT+CGG - chr2.2:44922137-44922156 MS.gene025273:intron 30.0%
AAGAAATTACCGCAAAGATT+AGG + chr2.2:44922115-44922134 None:intergenic 30.0%
ACAAAAGAACCTTACAACTA+TGG - chr2.2:44921210-44921229 MS.gene025273:intron 30.0%
ATTTCACTCGCTAAATGTAA+CGG - chr2.2:44921584-44921603 MS.gene025273:intron 30.0%
CAAAATGAAGAAAACTCACT+TGG + chr2.2:44921269-44921288 None:intergenic 30.0%
CGAAAAATTCGGAGAAATTA+AGG + chr2.2:44920580-44920599 None:intergenic 30.0%
TATGAACCAACTAAGAAGAA+AGG + chr2.2:44920697-44920716 None:intergenic 30.0%
TGCTATCAATCATTACTAAG+CGG + chr2.2:44921668-44921687 None:intergenic 30.0%
! ATTAACTTGATATGGTGCAT+TGG + chr2.2:44922410-44922429 None:intergenic 30.0%
! TTGTGTCAAATTGTTTTCCA+TGG + chr2.2:44921194-44921213 None:intergenic 30.0%
!! TTTTTAACTAGTTCCACCTA+GGG + chr2.2:44921463-44921482 None:intergenic 30.0%
!!! TTTTTTAACTAGTTCCACCT+AGG + chr2.2:44921464-44921483 None:intergenic 30.0%
AACTTCTGAATATTCCGATG+AGG + chr2.2:44920410-44920429 None:intergenic 35.0%
ACCAAATTAGGAGGAAATGA+AGG + chr2.2:44921983-44922002 None:intergenic 35.0%
ATTCGGATGACACGAAAAAT+TGG - chr2.2:44922154-44922173 MS.gene025273:intron 35.0%
ATTGTCAATTGTGCAGCTTT+AGG + chr2.2:44921830-44921849 None:intergenic 35.0%
CATGGTTGGAAACAAATTCA+TGG + chr2.2:44921176-44921195 None:intergenic 35.0%
TATCCTCTACAATTGCAGTA+TGG + chr2.2:44921627-44921646 None:intergenic 35.0%
TCCTAATTTGGTCAGAACTT+CGG - chr2.2:44921991-44922010 MS.gene025273:intron 35.0%
TCGGAATATTCAGAAGTTTC+CGG - chr2.2:44920412-44920431 MS.gene025273:CDS 35.0%
TGAGCAAATCCATAGTTGTA+AGG + chr2.2:44921222-44921241 None:intergenic 35.0%
TGCTTCAAAGATCTTTCTCT+TGG - chr2.2:44920520-44920539 MS.gene025273:intron 35.0%
! AATGATTTGCAGTGCTATCA+AGG - chr2.2:44921802-44921821 MS.gene025273:intron 35.0%
! ATCCGAATTTTACGTGTCAA+TGG + chr2.2:44922142-44922161 None:intergenic 35.0%
! ATGATTTGCAGTGCTATCAA+GGG - chr2.2:44921803-44921822 MS.gene025273:intron 35.0%
! CATGAATTTGTTTCCAACCA+TGG - chr2.2:44921174-44921193 MS.gene025273:intron 35.0%
! CTCATGGCTTTTGCTAATAA+AGG - chr2.2:44921908-44921927 MS.gene025273:intron 35.0%
! GCTGGCTTATTAACTTGATA+TGG + chr2.2:44922418-44922437 None:intergenic 35.0%
! GTCAAATTGTTTTCCATGGT+TGG + chr2.2:44921190-44921209 None:intergenic 35.0%
!! TGAGAGAAAGTTGTTTTCTC+CGG + chr2.2:44920460-44920479 None:intergenic 35.0%
AATTAAGGTTGCGTACCAGA+AGG + chr2.2:44920565-44920584 None:intergenic 40.0%
AATTAGGAGGAAATGAAGGC+TGG + chr2.2:44921979-44921998 None:intergenic 40.0%
ACCTAGATCCTCTACAGATA+CGG - chr2.2:44921509-44921528 MS.gene025273:intron 40.0%
ATCAATCATTACTAAGCGGC+TGG + chr2.2:44921664-44921683 None:intergenic 40.0%
ATGTTCACAGTAGGAACAAC+TGG + chr2.2:44922031-44922050 None:intergenic 40.0%
ATTAAGGTTGCGTACCAGAA+GGG + chr2.2:44920564-44920583 None:intergenic 40.0%
ATTACCGCAAAGATTAGGTC+TGG + chr2.2:44922110-44922129 None:intergenic 40.0%
ATTGGCAGATCTGCACATAA+AGG + chr2.2:44922205-44922224 None:intergenic 40.0%
GAATTGTTCAAAGCAGCTTG+TGG + chr2.2:44921247-44921266 None:intergenic 40.0%
GCCTTCATTTCCTCCTAATT+TGG - chr2.2:44921979-44921998 MS.gene025273:intron 40.0%
TTTCCACGAATTGACCCTTT+CGG + chr2.2:44921414-44921433 None:intergenic 40.0%
! AAGGTGTTCATTAATCGAGC+CGG + chr2.2:44922077-44922096 None:intergenic 40.0%
! ACCGAAGTTCTGACCAAATT+AGG + chr2.2:44921995-44922014 None:intergenic 40.0%
! GAAGTTCTGACCAAATTAGG+AGG + chr2.2:44921992-44922011 None:intergenic 40.0%
! TTCGTGGAAATAACTTGCAG+AGG - chr2.2:44921424-44921443 MS.gene025273:intron 40.0%
! TTCTGGAGAAGAGAAAGGAT+AGG - chr2.2:44922251-44922270 MS.gene025273:intron 40.0%
ACCGTATCTGTAGAGGATCT+AGG + chr2.2:44921513-44921532 None:intergenic 45.0%
AGGATTTAGATCCTCTCCCT+AGG - chr2.2:44921444-44921463 MS.gene025273:intron 45.0%
ATTTAGATCCTCTCCCTAGG+TGG - chr2.2:44921447-44921466 MS.gene025273:intron 45.0%
CATGAGCTCATGTGCTTTGT+CGG + chr2.2:44921894-44921913 None:intergenic 45.0%
CCTTCCAGACCTAATCTTTG+CGG - chr2.2:44922103-44922122 MS.gene025273:intron 45.0%
CTCTACAGATACGGTATGGA+CGG - chr2.2:44921518-44921537 MS.gene025273:intron 45.0%
GATCCTCTACAGATACGGTA+TGG - chr2.2:44921514-44921533 MS.gene025273:intron 45.0%
GCACCATACTGCAATTGTAG+AGG - chr2.2:44921621-44921640 MS.gene025273:CDS 45.0%
GGAGAAGAGAAAGGATAGGT+AGG - chr2.2:44922255-44922274 MS.gene025273:intron 45.0%
! TCGGTTTCTGGAGAAGAGAA+AGG - chr2.2:44922246-44922265 MS.gene025273:intron 45.0%
AACTAGTTCCACCTAGGGAG+AGG + chr2.2:44921458-44921477 None:intergenic 50.0%
ACATGCAACACAGACCCTTC+TGG - chr2.2:44920547-44920566 MS.gene025273:intron 50.0%
ACGAATTGACCCTTTCGGCA+AGG + chr2.2:44921409-44921428 None:intergenic 50.0%
AGCGAGGCTTTCCTCATGAT+TGG + chr2.2:44922223-44922242 None:intergenic 50.0%
AGTTTCCGGCAACAAACCAC+CGG - chr2.2:44920426-44920445 MS.gene025273:CDS 50.0%
CACAGTAGGAACAACTGGAG+CGG + chr2.2:44922026-44922045 None:intergenic 50.0%
CAGATACGGTATGGACGGTA+TGG - chr2.2:44921523-44921542 MS.gene025273:intron 50.0%
CCGCAAAGATTAGGTCTGGA+AGG + chr2.2:44922106-44922125 None:intergenic 50.0%
CGACAAAGCACATGAGCTCA+TGG - chr2.2:44921892-44921911 MS.gene025273:intron 50.0%
CGTCCATACCGTATCTGTAG+AGG + chr2.2:44921520-44921539 None:intergenic 50.0%
CTGGGGATGATGTTCACAGT+AGG + chr2.2:44922040-44922059 None:intergenic 50.0%
GTGCAGATCTGCCAATCATG+AGG - chr2.2:44922209-44922228 MS.gene025273:intron 50.0%
TGAGGAAAGCCTCGCTTCAT+CGG - chr2.2:44922227-44922246 MS.gene025273:intron 50.0%
TGAGTCCATGTCATGGCTTG+TGG - chr2.2:44922438-44922457 MS.gene025273:CDS 50.0%
TTCATTAATCGAGCCGGTGC+CGG + chr2.2:44922071-44922090 None:intergenic 50.0%
TTGCCGAAAGGGTCAATTCG+TGG - chr2.2:44921408-44921427 MS.gene025273:intron 50.0%
! GAGTCCATGTCATGGCTTGT+GGG - chr2.2:44922439-44922458 MS.gene025273:CDS 50.0%
! TCATTACTAAGCGGCTGGCT+CGG + chr2.2:44921659-44921678 None:intergenic 50.0%
!! TAGGTCTGGAAGGCACTTGA+AGG + chr2.2:44922096-44922115 None:intergenic 50.0%
AGCCAGCTGAGTCCATGTCA+TGG - chr2.2:44922431-44922450 MS.gene025273:CDS 55.0%
AGCCATGACATGGACTCAGC+TGG + chr2.2:44922436-44922455 None:intergenic 55.0%
AGCTCATGTGCTTTGTCGGC+AGG + chr2.2:44921890-44921909 None:intergenic 55.0%
ATAGCTGTCGCCTTGCCGAA+AGG - chr2.2:44921396-44921415 MS.gene025273:intron 55.0%
ATCATCCCCAGTACTGCCAC+CGG - chr2.2:44922049-44922068 MS.gene025273:intron 55.0%
CTCCAGAAACCGATGAAGCG+AGG + chr2.2:44922239-44922258 None:intergenic 55.0%
TAGCTGTCGCCTTGCCGAAA+GGG - chr2.2:44921397-44921416 MS.gene025273:intron 55.0%
TATCGTCACGCTAGCCGCTA+AGG + chr2.2:44921377-44921396 None:intergenic 55.0%
TGCACCCACAAGCCATGACA+TGG + chr2.2:44922446-44922465 None:intergenic 55.0%
! GTTTTCTCCGGTGACTTCGC+CGG + chr2.2:44920448-44920467 None:intergenic 55.0%
!! AGCCTCGCTTCATCGGTTTC+TGG - chr2.2:44922234-44922253 MS.gene025273:intron 55.0%
ATGGCGAGAGCGAGCCTTAG+CGG - chr2.2:44921360-44921379 MS.gene025273:intron 60.0%
CAAACCACCGGCGAAGTCAC+CGG - chr2.2:44920438-44920457 MS.gene025273:CDS 60.0%
! ATTAATCGAGCCGGTGCCGG+TGG + chr2.2:44922068-44922087 None:intergenic 60.0%
!! CTTCGCCGGTGGTTTGTTGC+CGG + chr2.2:44920434-44920453 None:intergenic 60.0%
TTCTCCGGTGACTTCGCCGG+TGG + chr2.2:44920445-44920464 None:intergenic 65.0%
CCCAGTACTGCCACCGGCAC+CGG - chr2.2:44922055-44922074 MS.gene025273:intron 70.0%
!! CCGGTGCCGGTGGCAGTACT+GGG + chr2.2:44922058-44922077 None:intergenic 70.0%
!! CGGTGCCGGTGGCAGTACTG+GGG + chr2.2:44922057-44922076 None:intergenic 70.0%
!! GCCGGTGCCGGTGGCAGTAC+TGG + chr2.2:44922059-44922078 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 44920400 44922483 44920400 ID=MS.gene025273
chr2.2 mRNA 44920400 44922483 44920400 ID=MS.gene025273.t1;Parent=MS.gene025273
chr2.2 exon 44922315 44922483 44922315 ID=MS.gene025273.t1.exon1;Parent=MS.gene025273.t1
chr2.2 CDS 44922315 44922483 44922315 ID=cds.MS.gene025273.t1;Parent=MS.gene025273.t1
chr2.2 exon 44921614 44921743 44921614 ID=MS.gene025273.t1.exon2;Parent=MS.gene025273.t1
chr2.2 CDS 44921614 44921743 44921614 ID=cds.MS.gene025273.t1;Parent=MS.gene025273.t1
chr2.2 exon 44920759 44921069 44920759 ID=MS.gene025273.t1.exon3;Parent=MS.gene025273.t1
chr2.2 CDS 44920759 44921069 44920759 ID=cds.MS.gene025273.t1;Parent=MS.gene025273.t1
chr2.2 exon 44920611 44920668 44920611 ID=MS.gene025273.t1.exon4;Parent=MS.gene025273.t1
chr2.2 CDS 44920611 44920668 44920611 ID=cds.MS.gene025273.t1;Parent=MS.gene025273.t1
chr2.2 exon 44920400 44920484 44920400 ID=MS.gene025273.t1.exon5;Parent=MS.gene025273.t1
chr2.2 CDS 44920400 44920484 44920400 ID=cds.MS.gene025273.t1;Parent=MS.gene025273.t1
Gene Sequence

>MS.gene025273

ATGTCTACCTCATCGGAATATTCAGAAGTTTCCGGCAACAAACCACCGGCGAAGTCACCGGAGAAAACAACTTTCTCTCAAACATGTAGTTTATTGAGTCAATATATTAAGGAAAAGGGTTGCTTCAAAGATCTTTCTCTTGGTATCACATGCAACACAGACCCTTCTGGTACGCAACCTTAATTTCTCCGAATTTTTCGTTTTTTGTTTCTGGGTTTTGTTCAATCTTTGAAATTTTTTTGTGGGTGTGATTAATTATTTTTTTAAAATTTGGTATCTTAATGGTTAATTATTCCTTTCTTCTTAGTTGGTTCATAATTATTAAATTCTAGTACTTAAGAGTAACGGTTAATTTGTGTCTATAGAGTAGACTATATCTATATATGTTCATTTATTTTTGTTAATTTATTTTTTCCTTTTTTGTAAGGTAGAACTATATCTAAATTTTCAAATATTTGCCATTTTAAATTATTCTTGTATGTTGTTTATTTTTATTTTGAATCCATGTTGTTTATTTTTTTTTTTTTGAGACTTTTAAAATTCATAGATCTAATTTCTAGCTCTAATTAATAGATAATTTGTTCATAATTCTAATTGTTATTATATACTAATTAGGTTGTTAAATTTGATAAGATTGTTATCTTGTTGTATAATTTTATTTGTTTTGTTAATTTTGTTAAATATTTTTTTTTGTGTAATATTGTTTGGTTTTTCTAAATTTGTTTTTTTTTTCTTTGCAGGGTCTTCTGAGACTTCTTCTCAATCTGCAACAACCATGAATTTGTTTCCAACCATGGAAAACAATTTGACACAAAAGAACCTTACAACTATGGATTTGCTCACTCCACAAGCTGCTTTGAACAATTCCAAGTGAGTTTTCTTCATTTTGTTCTGATTTCAATTAATTTCATTTCATACCAAATTAGGTTTGTAAAAAAATTAATTTATGAGTGATGACAAATGGCGAGAGCGAGCCTTAGCGGCTAGCGTGACGATATAGCTGTCGCCTTGCCGAAAGGGTCAATTCGTGGAAATAACTTGCAGAGGATTTAGATCCTCTCCCTAGGTGGAACTAGTTAAAAAAATAAATTTGATGGAGAGGCTCACACACCTAGATCCTCTACAGATACGGTATGGACGGTATGGTGCTACATCTGTAAGAACGTTAGATTTAGAGAGATCAAATTTCACTCGCTAAATGTAACGGTCGCTACAGCTGCAGCACCATACTGCAATTGTAGAGGATAAGTTGTGAACCGAGCCAGCCGCTTAGTAATGATTGATAGCAATTTATAAATGTTGTGCTGATTTAATAATCTTGATATAGTTCGAGATATCAAAACCATTAGTTGATTATTAATTTTTTTTTCTTCGATTAGAGACTAATATATGATGTTGTATGAATGATTTGCAGTGCTATCAAGGGACCTAAAGCTGCACAATTGACAATGTTTTATAATGGTCAAGTTATTGTATTTGACGATTTTCCTGCCGACAAAGCACATGAGCTCATGGCTTTTGCTAATAAAGGAATCTCTCAAAGTCAGAACAATTCTGTGTACACTTACACACAGAGCCAGCCTTCATTTCCTCCTAATTTGGTCAGAACTTCGGTTAACACAACCGCTCCAGTTGTTCCTACTGTGAACATCATCCCCAGTACTGCCACCGGCACCGGCTCGATTAATGAACACCTTCAAGTGCCTTCCAGACCTAATCTTTGCGGTAATTTCTTGAAACCATTGACACGTAAAATTCGGATGACACGAAAAATTGGATCATGTTTTTCTGATCATGAATTTCCTTTATGTGCAGATCTGCCAATCATGAGGAAAGCCTCGCTTCATCGGTTTCTGGAGAAGAGAAAGGATAGGTAGGCACTCTCGATTAAATTAAAACTTGTAAATGAACTATTTTTTACATCTTTTCTAACTTAGCTTGTTGATCAAGTATTTTTGCTGAGATAATAATAATTTGTTTCATTGTTGTGTATTTACAGAATTGCTGCCAATGCACCATATCAAGTTAATAAGCCAGCTGAGTCCATGTCATGGCTTGTGGGTGCAAAATCAACTCAAATTTGA
Protein sequence

>MS.gene025273.t1

MSTSSEYSEVSGNKPPAKSPEKTTFSQTCSLLSQYIKEKGCFKDLSLGITCNTDPSGSSETSSQSATTMNLFPTMENNLTQKNLTTMDLLTPQAALNNSNAIKGPKAAQLTMFYNGQVIVFDDFPADKAHELMAFANKGISQSQNNSVYTYTQSQPSFPPNLVRTSVNTTAPVVPTVNIIPSTATGTGSINEHLQVPSRPNLCDLPIMRKASLHRFLEKRKDRIAANAPYQVNKPAESMSWLVGAKSTQI