AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025386.t1 MTR_2g044910 93.780 209 11 1 1 209 1 207 1.17e-141 394
MS.gene025386.t1 MTR_2g044910 93.976 166 8 1 44 209 1 164 1.01e-110 314
MS.gene025386.t1 MTR_2g044910 85.897 156 15 3 1 154 1 151 2.97e-88 257
MS.gene025386.t1 MTR_4g124360 58.955 134 47 3 38 168 25 153 2.39e-44 146
MS.gene025386.t1 MTR_4g124360 60.606 132 43 4 56 184 5 130 1.07e-40 135
MS.gene025386.t1 MTR_5g013515 35.833 120 66 4 57 176 13 121 1.50e-15 70.9
MS.gene025386.t1 MTR_2g042900 31.538 130 62 3 58 168 112 233 6.54e-14 68.9
MS.gene025386.t1 MTR_5g013530 33.333 114 59 3 58 171 22 118 2.99e-12 62.4
MS.gene025386.t1 MTR_4g124960 31.148 122 47 3 49 168 90 176 8.74e-12 62.0
MS.gene025386.t1 MTR_5g013530 33.333 114 59 3 58 171 44 140 9.91e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025386.t1 AT5G20900 51.724 116 48 4 58 171 57 166 3.55e-29 107
MS.gene025386.t1 AT3G43440 38.760 129 53 4 57 183 130 234 7.06e-15 71.2
MS.gene025386.t1 AT3G43440 36.154 130 57 4 56 183 43 148 1.51e-14 68.6
MS.gene025386.t1 AT1G74950 36.800 125 48 4 59 168 120 228 4.44e-13 66.6
MS.gene025386.t1 AT1G19180 35.043 117 62 3 54 168 56 160 4.46e-12 62.8
MS.gene025386.t1 AT1G19180 34.746 118 61 4 54 168 122 226 8.98e-12 62.8
MS.gene025386.t1 AT1G19180 34.746 118 61 4 54 168 122 226 8.98e-12 62.8

Find 47 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTCCTGTTGCTGTTGA+TGG 0.258403 2.2:-42210376 MS.gene025386:CDS
CAGCTTACCATCTTCTATAA+TGG 0.263317 2.2:-42207450 MS.gene025386:CDS
TTCCTTCACCTGTTCCTTCA+AGG 0.303820 2.2:-42207210 MS.gene025386:CDS
TCCTTCATTGAAACCGAATC+TGG 0.348326 2.2:+42206760 None:intergenic
TTTAGGTCAATGCCAGCATC+TGG 0.377821 2.2:-42207501 MS.gene025386:intron
TGCTGACAATTCGCCAGATT+CGG 0.379407 2.2:-42206773 MS.gene025386:CDS
AGCTTACCATCTTCTATAAT+GGG 0.398580 2.2:-42207449 MS.gene025386:CDS
CTGCAGATAGAACTCTTTGC+AGG 0.418330 2.2:+42207130 None:intergenic
GCCAGATTCGGTTTCAATGA+AGG 0.420770 2.2:-42206761 MS.gene025386:CDS
ACAGGTGAAGGAACGGGAAT+GGG 0.422899 2.2:+42207220 None:intergenic
AATGGATGGTGTTACTGTTA+AGG 0.443386 2.2:-42210428 MS.gene025386:CDS
GCTAGTTCCAACATGGTTGA+GGG 0.452791 2.2:-42210352 MS.gene025386:CDS
AGTGAATGTCTGCGTGCAAT+TGG 0.453687 2.2:+42206974 None:intergenic
GTTGGCTGAAATTCTTCCAT+TGG 0.460471 2.2:+42206736 None:intergenic
CTAGCACCATCAACAGCAAC+AGG 0.467485 2.2:+42210370 None:intergenic
CTTCTTCGTTTCACTAGACT+TGG 0.471119 2.2:+42207258 None:intergenic
TGATGGAATTCCTGCAGAAA+AGG 0.477638 2.2:-42207412 MS.gene025386:intron
GGGAGTATTTGTATCTATGA+TGG 0.478209 2.2:-42207429 MS.gene025386:CDS
TGCTAGTTCCAACATGGTTG+AGG 0.478224 2.2:-42210353 MS.gene025386:CDS
GCAGTTCCATGTGGCGAAGA+AGG 0.484372 2.2:+42207187 None:intergenic
CTGCAAAGAGTTCTATCTGC+AGG 0.484476 2.2:-42207129 MS.gene025386:CDS
TCTAGAACAGTAAACTTCTC+TGG 0.487430 2.2:+42210400 None:intergenic
TGTGGCGAAGAAGGCCTTGA+AGG 0.491077 2.2:+42207196 None:intergenic
ATAGAACTCTTTGCAGGAAA+GGG 0.495560 2.2:+42207136 None:intergenic
AACAGGTGAAGGAACGGGAA+TGG 0.503659 2.2:+42207219 None:intergenic
AATACTCCCATTATAGAAGA+TGG 0.513403 2.2:+42207443 None:intergenic
TTCTTCAGAAGCGTCGAGAC+AGG 0.516859 2.2:-42206943 MS.gene025386:intron
ACTGGATTCATTCCAGATGC+TGG 0.517894 2.2:+42207489 None:intergenic
GAAGGAACAGGTGAAGGAAC+GGG 0.518842 2.2:+42207214 None:intergenic
GAAGAAGGCCTTGAAGGAAC+AGG 0.524051 2.2:+42207202 None:intergenic
GCTCAAGATGCAGATATAGT+TGG 0.526009 2.2:+42206718 None:intergenic
TGAGTTGTATTAGCAATCAC+TGG 0.534299 2.2:+42207471 None:intergenic
TCAAGGCCTTCTTCGCCACA+TGG 0.540794 2.2:-42207193 MS.gene025386:CDS
AGTTCTATCTGCAGGCTGCA+AGG 0.555717 2.2:-42207121 MS.gene025386:intron
GATAGAACTCTTTGCAGGAA+AGG 0.557564 2.2:+42207135 None:intergenic
GGCCTTGAAGGAACAGGTGA+AGG 0.574210 2.2:+42207208 None:intergenic
CATTGACCCCTCAACCATGT+TGG 0.577350 2.2:+42210345 None:intergenic
TGAAGGAACAGGTGAAGGAA+CGG 0.580717 2.2:+42207213 None:intergenic
GGTTTCAATGAAGGAACCAA+TGG 0.581761 2.2:-42206752 MS.gene025386:CDS
AACAAAGGTAGCTGACAACA+TGG 0.591184 2.2:-42206809 MS.gene025386:CDS
GCAGGAAAGGGAGGTGCCTG+CGG 0.600459 2.2:+42207148 None:intergenic
GACAACATGGAGAACAACTT+CGG 0.616517 2.2:-42206796 MS.gene025386:CDS
TGATAATATCACTTCACCGC+AGG 0.617304 2.2:-42207164 MS.gene025386:CDS
GAACTCTTTGCAGGAAAGGG+AGG 0.634573 2.2:+42207139 None:intergenic
TGATGGTGCTAGTTCCAACA+TGG 0.653805 2.2:-42210359 MS.gene025386:CDS
CTAGTTCCAACATGGTTGAG+GGG 0.662811 2.2:-42210351 MS.gene025386:CDS
ATATTATCAGCAGTTCCATG+TGG 0.736827 2.2:+42207178 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTATAAAAAATTGATATA+AGG - chr2.2:42209224-42209243 MS.gene025386:intron 10.0%
!! AAAATTGGAAACAAAAAAAA+AGG + chr2.2:42206949-42206968 None:intergenic 15.0%
!! AAATTGGAAACAAAAAAAAA+GGG + chr2.2:42206948-42206967 None:intergenic 15.0%
!! ATAATTTATGTTTGTATTAC+AGG - chr2.2:42210280-42210299 MS.gene025386:intron 15.0%
!!! ATAAAAAAAACATGCTTTTT+TGG + chr2.2:42206868-42206887 None:intergenic 15.0%
!!! GTTGAATGTTTTTTTTTTTT+TGG - chr2.2:42207020-42207039 MS.gene025386:intron 15.0%
!!! TAACTTGGTTATTTTAATTT+TGG - chr2.2:42206998-42207017 MS.gene025386:intron 15.0%
!!! TCTACTAAAAACAAAATTTT+CGG - chr2.2:42207230-42207249 MS.gene025386:CDS 15.0%
!! AAAACTAGAACTATAGATTA+TGG + chr2.2:42210251-42210270 None:intergenic 20.0%
!! AAAATTGTTCTAATGTGTTA+AGG - chr2.2:42207166-42207185 MS.gene025386:CDS 20.0%
!! AAATTGTTCTAATGTGTTAA+GGG - chr2.2:42207167-42207186 MS.gene025386:CDS 20.0%
!! AAGAACTTAACGATTAATTA+CGG + chr2.2:42207589-42207608 None:intergenic 20.0%
!! ATCAAAACAGAAACAAAAAT+TGG + chr2.2:42206964-42206983 None:intergenic 20.0%
!! ATCTTGGTTATTTAAAAAGT+TGG + chr2.2:42208409-42208428 None:intergenic 20.0%
!! TATATTCAATTTGACGAAAA+AGG + chr2.2:42208457-42208476 None:intergenic 20.0%
!! TGGCATAAAATATTTCAATT+CGG + chr2.2:42209153-42209172 None:intergenic 20.0%
!! TTTCGTCAAATTGAATATAA+AGG - chr2.2:42208458-42208477 MS.gene025386:intron 20.0%
!!! ACTAGATATTGTTTCATTTT+AGG + chr2.2:42209025-42209044 None:intergenic 20.0%
!!! CATAATTTTACTTTTCTGAT+CGG - chr2.2:42208701-42208720 MS.gene025386:intron 20.0%
!!! TAGCTAAGAGTTTAAAATAA+AGG + chr2.2:42208983-42209002 None:intergenic 20.0%
! AACCAAAATATATGTATCAG+TGG + chr2.2:42209303-42209322 None:intergenic 25.0%
! AACTATAGATTATGGTAACA+TGG + chr2.2:42210243-42210262 None:intergenic 25.0%
! AATGGAATAAGTAATTACAG+TGG + chr2.2:42208817-42208836 None:intergenic 25.0%
! ACACAAAAACTGTTATATCA+CGG - chr2.2:42208945-42208964 MS.gene025386:intron 25.0%
! ACACAAATTGAAACCATTTA+AGG + chr2.2:42207104-42207123 None:intergenic 25.0%
! ACTATAGATTATGGTAACAT+GGG + chr2.2:42210242-42210261 None:intergenic 25.0%
! AGTAGATCAAAGAAAACTTT+AGG + chr2.2:42207294-42207313 None:intergenic 25.0%
! ATCTACTTAAATATTACGTG+CGG + chr2.2:42207459-42207478 None:intergenic 25.0%
! ATTAGACTCTATTTACTAAC+AGG - chr2.2:42209833-42209852 MS.gene025386:intron 25.0%
! ATTTCATTCAAAACAGAACA+GGG + chr2.2:42208633-42208652 None:intergenic 25.0%
! CGGTTAAATTATTTATCCTA+TGG - chr2.2:42207250-42207269 MS.gene025386:CDS 25.0%
! GCAAATATACGAAATATATG+AGG - chr2.2:42209443-42209462 MS.gene025386:intron 25.0%
! TAAAATAAAGGTTTGTGATG+CGG + chr2.2:42208971-42208990 None:intergenic 25.0%
! TAGTAGTTAAGAGTTGTTAA+GGG - chr2.2:42207719-42207738 MS.gene025386:intron 25.0%
! TAGTTGAAGACATAGTTAAT+AGG + chr2.2:42210062-42210081 None:intergenic 25.0%
! TATATGTATCAGTGGTTAAT+TGG + chr2.2:42209295-42209314 None:intergenic 25.0%
! TCAGAAAAGTAAAATTATGC+TGG + chr2.2:42208700-42208719 None:intergenic 25.0%
! TGATTCATTGCATAATAGAA+CGG - chr2.2:42207515-42207534 MS.gene025386:intron 25.0%
! TGGATTACTAACAATATCAT+AGG - chr2.2:42207966-42207985 MS.gene025386:intron 25.0%
! TTAGTAGTTAAGAGTTGTTA+AGG - chr2.2:42207718-42207737 MS.gene025386:intron 25.0%
! TTATGTTTGTATTACAGGTT+GGG - chr2.2:42210285-42210304 MS.gene025386:intron 25.0%
! TTCTATTATGCAATGAATCA+GGG + chr2.2:42207515-42207534 None:intergenic 25.0%
! TTGTGAAAATAGAAAAGAGT+AGG + chr2.2:42207915-42207934 None:intergenic 25.0%
! TTTATGTTTGTATTACAGGT+TGG - chr2.2:42210284-42210303 MS.gene025386:intron 25.0%
!! AACCACTGATACATATATTT+TGG - chr2.2:42209298-42209317 MS.gene025386:intron 25.0%
!! ACCATTTAAGGTACAAAAAT+TGG + chr2.2:42207092-42207111 None:intergenic 25.0%
!! TAATTTTGCTTATGACAGAA+AGG + chr2.2:42208083-42208102 None:intergenic 25.0%
!! TCCAATTTTTGTACCTTAAA+TGG - chr2.2:42207088-42207107 MS.gene025386:intron 25.0%
!! TTCGGTTAACTATTTTCAAA+AGG + chr2.2:42209135-42209154 None:intergenic 25.0%
!! TTGACTTAATTCCACTATTT+TGG - chr2.2:42209255-42209274 MS.gene025386:intron 25.0%
!! TTTCTTTTCAATTCAACTCT+TGG - chr2.2:42207946-42207965 MS.gene025386:intron 25.0%
!!! ATGAAAAAGTATTAAGTCCA+AGG + chr2.2:42208382-42208401 None:intergenic 25.0%
!!! GTTTTCTAGAATAGAGTTTA+TGG + chr2.2:42209479-42209498 None:intergenic 25.0%
!!! TCTGTTTTGAATGAAATCAT+TGG - chr2.2:42208636-42208655 MS.gene025386:intron 25.0%
AAATAACCAAGATCATTGCT+TGG - chr2.2:42208416-42208435 MS.gene025386:intron 30.0%
AACAATCCACCATATTAACA+TGG + chr2.2:42208248-42208267 None:intergenic 30.0%
AATACTCCCATTATAGAAGA+TGG + chr2.2:42209707-42209726 None:intergenic 30.0%
AATCTGATTGTACAGTAGAA+AGG + chr2.2:42207676-42207695 None:intergenic 30.0%
AATTGTTGAGGAGAAAAGTA+GGG + chr2.2:42207364-42207383 None:intergenic 30.0%
ACAAAAAAAAAGGGTCTTGA+AGG + chr2.2:42206939-42206958 None:intergenic 30.0%
ACATATGTTTCAGCAAGATT+CGG - chr2.2:42208837-42208856 MS.gene025386:intron 30.0%
AGCTTACCATCTTCTATAAT+GGG - chr2.2:42209698-42209717 MS.gene025386:intron 30.0%
ATGAAGTTACAATAGCAATC+CGG + chr2.2:42207635-42207654 None:intergenic 30.0%
ATTGCTTGGCAAATATGATA+CGG - chr2.2:42208430-42208449 MS.gene025386:intron 30.0%
ATTGGTGGAAATTGTAACTT+TGG + chr2.2:42209501-42209520 None:intergenic 30.0%
CTACTTTAGTCATCAAAAAC+TGG - chr2.2:42209604-42209623 MS.gene025386:intron 30.0%
GATTTCATTCAAAACAGAAC+AGG + chr2.2:42208634-42208653 None:intergenic 30.0%
GCAAAATCATTGTATAACAG+TGG + chr2.2:42209192-42209211 None:intergenic 30.0%
GTTCTATTATGCAATGAATC+AGG + chr2.2:42207516-42207535 None:intergenic 30.0%
GTTGTTAAGGGATAGAATAA+AGG - chr2.2:42207731-42207750 MS.gene025386:intron 30.0%
TCTTGCTGAAACATATGTAA+TGG + chr2.2:42208835-42208854 None:intergenic 30.0%
TGAATGATTACTCACTAACT+TGG - chr2.2:42206983-42207002 MS.gene025386:intron 30.0%
TGTAGATGAACAATGAACTA+AGG - chr2.2:42207056-42207075 MS.gene025386:intron 30.0%
TTACAATTTCCACCAATATC+AGG - chr2.2:42209504-42209523 MS.gene025386:intron 30.0%
TTAGGAGAAACCATTAAACA+AGG + chr2.2:42207276-42207295 None:intergenic 30.0%
TTATCTTCTTACTTGAGAAC+CGG - chr2.2:42207613-42207632 MS.gene025386:intron 30.0%
TTCAAAACACACCAAAATAG+TGG + chr2.2:42209269-42209288 None:intergenic 30.0%
! CTGTTATACAATGATTTTGC+TGG - chr2.2:42209192-42209211 MS.gene025386:intron 30.0%
! TGATTGAACAACAAAAGCAT+AGG - chr2.2:42208537-42208556 MS.gene025386:intron 30.0%
! TTTTTCTGAATTCTGAACCT+TGG - chr2.2:42208362-42208381 MS.gene025386:intron 30.0%
!! ATGTTAATATGGTGGATTGT+TGG - chr2.2:42208247-42208266 MS.gene025386:intron 30.0%
!! CTTTGATCTACTTTTCTTAC+TGG - chr2.2:42207302-42207321 MS.gene025386:intron 30.0%
!! GTAACTTCATTTTAGTCTGT+TGG - chr2.2:42207645-42207664 MS.gene025386:intron 30.0%
!!! ACTATTTTGGTGTGTTTTGA+AGG - chr2.2:42209268-42209287 MS.gene025386:intron 30.0%
!!! AGATGTATCGTTGTTTTTGA+TGG - chr2.2:42208674-42208693 MS.gene025386:intron 30.0%
!!! CTATTTTGGTGTGTTTTGAA+GGG - chr2.2:42209269-42209288 MS.gene025386:intron 30.0%
!!! TTTTGGTTTTTTGGCTTTTC+TGG - chr2.2:42208314-42208333 MS.gene025386:intron 30.0%
AAACCATTAAACAAGGCCAT+AGG + chr2.2:42207269-42207288 None:intergenic 35.0%
AAATGTGATTAACCAGCGAA+TGG + chr2.2:42209071-42209090 None:intergenic 35.0%
AGAGTAGTTAGTTAGCTGTT+CGG - chr2.2:42207787-42207806 MS.gene025386:intron 35.0%
AGCATAGATAAGCCTGATAT+TGG + chr2.2:42209519-42209538 None:intergenic 35.0%
ATAGAACTCTTTGCAGGAAA+GGG + chr2.2:42210014-42210033 None:intergenic 35.0%
ATAGATAAGCCTGATATTGG+TGG + chr2.2:42209516-42209535 None:intergenic 35.0%
ATAGATTATGGTAACATGGG+AGG + chr2.2:42210239-42210258 None:intergenic 35.0%
ATATTATCAGCAGTTCCATG+TGG + chr2.2:42209972-42209991 None:intergenic 35.0%
CAATTGTTGAGGAGAAAAGT+AGG + chr2.2:42207365-42207384 None:intergenic 35.0%
CACAACACAACCTGTTATTA+AGG + chr2.2:42208916-42208935 None:intergenic 35.0%
CACACGAGACATATAGATTA+TGG + chr2.2:42210124-42210143 None:intergenic 35.0%
CAGCTTACCATCTTCTATAA+TGG - chr2.2:42209697-42209716 MS.gene025386:intron 35.0%
CATAATCTATATGTCTCGTG+TGG - chr2.2:42210122-42210141 MS.gene025386:intron 35.0%
CGGTCTATGTCAGATATAAT+AGG + chr2.2:42208220-42208239 None:intergenic 35.0%
CTAAATCAATGAAGACAGTG+TGG - chr2.2:42207995-42208014 MS.gene025386:intron 35.0%
CTTAATAACAGGTTGTGTTG+TGG - chr2.2:42208914-42208933 MS.gene025386:intron 35.0%
GAAAAGATGCAGAACAATAC+AGG + chr2.2:42208028-42208047 None:intergenic 35.0%
GAAACAAACACCAATTGTTG+AGG + chr2.2:42207376-42207395 None:intergenic 35.0%
GCTCATAGTCATTGATAAGT+GGG - chr2.2:42207431-42207450 MS.gene025386:CDS 35.0%
GGCTGAATTCATGCATATTT+CGG - chr2.2:42208861-42208880 MS.gene025386:intron 35.0%
TAACGATTAATTACGGAGCT+CGG + chr2.2:42207582-42207601 None:intergenic 35.0%
TATTTGCCAAGCAATGATCT+TGG + chr2.2:42208425-42208444 None:intergenic 35.0%
TGAGTTGTATTAGCAATCAC+TGG + chr2.2:42209679-42209698 None:intergenic 35.0%
! ACAAAAGCATAGGAAACCAT+TGG - chr2.2:42208547-42208566 MS.gene025386:intron 35.0%
! ACCATATTAACATGGTGATG+CGG + chr2.2:42208240-42208259 None:intergenic 35.0%
! ATGGATAACACGGGATATTT+TGG - chr2.2:42208297-42208316 MS.gene025386:intron 35.0%
! CAAAAGCATAGGAAACCATT+GGG - chr2.2:42208548-42208567 MS.gene025386:intron 35.0%
! GCTTTGTAACTTCCTCTTTT+AGG - chr2.2:42209629-42209648 MS.gene025386:intron 35.0%
! GTCAGATATAATAGGTGTTG+TGG + chr2.2:42208212-42208231 None:intergenic 35.0%
! TCTAGAACAGTAAACTTCTC+TGG + chr2.2:42206750-42206769 None:intergenic 35.0%
!! ACTTCATTTTAGTCTGTTGG+TGG - chr2.2:42207648-42207667 MS.gene025386:intron 35.0%
!! CTACTTTTCTCCTCAACAAT+TGG - chr2.2:42207363-42207382 MS.gene025386:intron 35.0%
!! GGGAGTATTTGTATCTATGA+TGG - chr2.2:42209718-42209737 MS.gene025386:intron 35.0%
!! TATCCTATGGCCTTGTTTAA+TGG - chr2.2:42207263-42207282 MS.gene025386:CDS 35.0%
!!! ATTTTGATTGTGTCGCAACA+TGG + chr2.2:42209810-42209829 None:intergenic 35.0%
!!! CACGGGATATTTTGGTTTTT+TGG - chr2.2:42208305-42208324 MS.gene025386:intron 35.0%
!!! TTGTCAGCTACCTTTGTTTT+TGG + chr2.2:42210336-42210355 None:intergenic 35.0%
AACAAAGGTAGCTGACAACA+TGG - chr2.2:42210338-42210357 MS.gene025386:CDS 40.0%
AATCATCTCAGTGTAGCGTA+GGG + chr2.2:42209764-42209783 None:intergenic 40.0%
ACCGCATCACCATGTTAATA+TGG - chr2.2:42208236-42208255 MS.gene025386:intron 40.0%
ACCTTGGTATGTATCCTTCA+CGG - chr2.2:42208053-42208072 MS.gene025386:intron 40.0%
ACGTCTAACCAAATCGAACA+CGG - chr2.2:42208770-42208789 MS.gene025386:intron 40.0%
AGCCTAGAAAACTCAGTACA+TGG + chr2.2:42209351-42209370 None:intergenic 40.0%
AGTAGGAGTCTCTTCTTCAA+GGG + chr2.2:42207898-42207917 None:intergenic 40.0%
AGTTAGTTAGCTGTTCGGTT+AGG - chr2.2:42207792-42207811 MS.gene025386:intron 40.0%
CTTCTTCGTTTCACTAGACT+TGG + chr2.2:42209892-42209911 None:intergenic 40.0%
GACAACATGGAGAACAACTT+CGG - chr2.2:42210351-42210370 MS.gene025386:CDS 40.0%
GATAGAACTCTTTGCAGGAA+AGG + chr2.2:42210015-42210034 None:intergenic 40.0%
GCAGTCAAATACTGTTGATG+TGG - chr2.2:42208890-42208909 MS.gene025386:intron 40.0%
GCATCACCATGTTAATATGG+TGG - chr2.2:42208239-42208258 MS.gene025386:intron 40.0%
GGCTCATAGTCATTGATAAG+TGG - chr2.2:42207430-42207449 MS.gene025386:CDS 40.0%
GGTTTCAATGAAGGAACCAA+TGG - chr2.2:42210395-42210414 MS.gene025386:CDS 40.0%
GTGGAAGCTCATAGAAAATG+TGG + chr2.2:42209173-42209192 None:intergenic 40.0%
GTTGGCTGAAATTCTTCCAT+TGG + chr2.2:42210414-42210433 None:intergenic 40.0%
TAATCATCTCAGTGTAGCGT+AGG + chr2.2:42209765-42209784 None:intergenic 40.0%
TATGTTTCAGCAAGATTCGG+AGG - chr2.2:42208840-42208859 MS.gene025386:intron 40.0%
TCATTCAAAACAGAACAGGG+AGG + chr2.2:42208630-42208649 None:intergenic 40.0%
TCCTTCATTGAAACCGAATC+TGG + chr2.2:42210390-42210409 None:intergenic 40.0%
TGATAATATCACTTCACCGC+AGG - chr2.2:42209983-42210002 MS.gene025386:intron 40.0%
TGATGGAATTCCTGCAGAAA+AGG - chr2.2:42209735-42209754 MS.gene025386:intron 40.0%
TGCTTCTGTGTTTGTTTGAG+TGG - chr2.2:42209535-42209554 MS.gene025386:intron 40.0%
TTGAATTCAAGACCATTCGC+TGG - chr2.2:42209056-42209075 MS.gene025386:intron 40.0%
TTGCAGAGACTAAATCCCAA+TGG + chr2.2:42208566-42208585 None:intergenic 40.0%
TTTCGTGTTTGTGTTGAGCA+TGG - chr2.2:42207409-42207428 MS.gene025386:intron 40.0%
! ACTTTTGTCCGTGTTCGATT+TGG + chr2.2:42208781-42208800 None:intergenic 40.0%
! ATAATAGGTGTTGTGGCCTA+TGG + chr2.2:42208205-42208224 None:intergenic 40.0%
! CTCCATGTACTGAGTTTTCT+AGG - chr2.2:42209346-42209365 MS.gene025386:intron 40.0%
! GTTGATGTGGCCTTAATAAC+AGG - chr2.2:42208903-42208922 MS.gene025386:intron 40.0%
! TGCATCTTTTCATCGAACCT+TGG - chr2.2:42208037-42208056 MS.gene025386:intron 40.0%
ACCGTGAAGGATACATACCA+AGG + chr2.2:42208057-42208076 None:intergenic 45.0%
ACTGGATTCATTCCAGATGC+TGG + chr2.2:42209661-42209680 None:intergenic 45.0%
AGTGAATGTCTGCGTGCAAT+TGG + chr2.2:42210176-42210195 None:intergenic 45.0%
CATAATCTGCCATCTGCAGA+TGG - chr2.2:42208278-42208297 MS.gene025386:intron 45.0%
CATCTGCAGATGGATAACAC+GGG - chr2.2:42208288-42208307 MS.gene025386:intron 45.0%
CATCTGCAGATGGCAGATTA+TGG + chr2.2:42208280-42208299 None:intergenic 45.0%
CCATCTGCAGATGGATAACA+CGG - chr2.2:42208287-42208306 MS.gene025386:intron 45.0%
CTAGTTCCAACATGGTTGAG+GGG - chr2.2:42206796-42206815 MS.gene025386:CDS 45.0%
CTGCAAAGAGTTCTATCTGC+AGG - chr2.2:42210018-42210037 MS.gene025386:intron 45.0%
CTGCAGATAGAACTCTTTGC+AGG + chr2.2:42210020-42210039 None:intergenic 45.0%
GAGTAGGAGTCTCTTCTTCA+AGG + chr2.2:42207899-42207918 None:intergenic 45.0%
GCTAGTTCCAACATGGTTGA+GGG - chr2.2:42206795-42206814 MS.gene025386:CDS 45.0%
GTACATCTCTAACAGTCGAG+TGG + chr2.2:42207549-42207568 None:intergenic 45.0%
GTAGGAGTCTCTTCTTCAAG+GGG + chr2.2:42207897-42207916 None:intergenic 45.0%
GTTAGAGATGTACGCGCAAT+TGG - chr2.2:42207556-42207575 MS.gene025386:intron 45.0%
TCCTTCCTCGCCAAAAACAA+AGG - chr2.2:42210323-42210342 MS.gene025386:intron 45.0%
TGAAGGAACAGGTGAAGGAA+CGG + chr2.2:42209937-42209956 None:intergenic 45.0%
TGCTAGTTCCAACATGGTTG+AGG - chr2.2:42206794-42206813 MS.gene025386:CDS 45.0%
TGCTGACAATTCGCCAGATT+CGG - chr2.2:42210374-42210393 MS.gene025386:CDS 45.0%
TTCCTTCACCTGTTCCTTCA+AGG - chr2.2:42209937-42209956 MS.gene025386:intron 45.0%
TTTAGGTCAATGCCAGCATC+TGG - chr2.2:42209646-42209665 MS.gene025386:intron 45.0%
! GCCAGATTCGGTTTCAATGA+AGG - chr2.2:42210386-42210405 MS.gene025386:CDS 45.0%
! TCTTCTCCTGTTGCTGTTGA+TGG - chr2.2:42206771-42206790 MS.gene025386:CDS 45.0%
! TGATGGTGCTAGTTCCAACA+TGG - chr2.2:42206788-42206807 MS.gene025386:CDS 45.0%
!! TGCTGGCATTGACCTAAAAG+AGG + chr2.2:42209644-42209663 None:intergenic 45.0%
!!! ACCTTTGTTTTTGGCGAGGA+AGG + chr2.2:42210327-42210346 None:intergenic 45.0%
!!! AGCTACCTTTGTTTTTGGCG+AGG + chr2.2:42210331-42210350 None:intergenic 45.0%
!!! GTTTTTGGCGAGGAAGGATA+AGG + chr2.2:42210321-42210340 None:intergenic 45.0%
AACAGGTGAAGGAACGGGAA+TGG + chr2.2:42209931-42209950 None:intergenic 50.0%
ACAGGTGAAGGAACGGGAAT+GGG + chr2.2:42209930-42209949 None:intergenic 50.0%
AGTTCTATCTGCAGGCTGCA+AGG - chr2.2:42210026-42210045 MS.gene025386:intron 50.0%
CATTGACCCCTCAACCATGT+TGG + chr2.2:42206805-42206824 None:intergenic 50.0%
CCGTGTTATCCATCTGCAGA+TGG + chr2.2:42208290-42208309 None:intergenic 50.0%
CTAGCACCATCAACAGCAAC+AGG + chr2.2:42206780-42206799 None:intergenic 50.0%
CTGCAGATGGCAGATTATGG+TGG + chr2.2:42208277-42208296 None:intergenic 50.0%
GAACTCTTTGCAGGAAAGGG+AGG + chr2.2:42210011-42210030 None:intergenic 50.0%
GAAGAAGGCCTTGAAGGAAC+AGG + chr2.2:42209948-42209967 None:intergenic 50.0%
GAAGGAACAGGTGAAGGAAC+GGG + chr2.2:42209936-42209955 None:intergenic 50.0%
GACAGAAAGGTCAACCGTGA+AGG + chr2.2:42208070-42208089 None:intergenic 50.0%
!! TTCTTCAGAAGCGTCGAGAC+AGG - chr2.2:42210204-42210223 MS.gene025386:intron 50.0%
GCAGTTCCATGTGGCGAAGA+AGG + chr2.2:42209963-42209982 None:intergenic 55.0%
GGCCTTGAAGGAACAGGTGA+AGG + chr2.2:42209942-42209961 None:intergenic 55.0%
TCAAGGCCTTCTTCGCCACA+TGG - chr2.2:42209954-42209973 MS.gene025386:intron 55.0%
TGTGGCGAAGAAGGCCTTGA+AGG + chr2.2:42209954-42209973 None:intergenic 55.0%
! CGTAGGGCTACCTTTTCTGC+AGG + chr2.2:42209748-42209767 None:intergenic 55.0%
TGAGCACCACCAATGTGCCG+TGG - chr2.2:42208173-42208192 MS.gene025386:intron 60.0%
CAGCTGCCACGGCACATTGG+TGG + chr2.2:42208182-42208201 None:intergenic 65.0%
GTGGCCTATGGCAGCTGCCA+CGG + chr2.2:42208193-42208212 None:intergenic 65.0%
TGGCAGCTGCCACGGCACAT+TGG + chr2.2:42208185-42208204 None:intergenic 65.0%
TGTGCCGTGGCAGCTGCCAT+AGG - chr2.2:42208186-42208205 MS.gene025386:intron 65.0%
!! GCAGGAAAGGGAGGTGCCTG+CGG + chr2.2:42210002-42210021 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 42206720 42210449 42206720 ID=MS.gene025386
chr2.2 mRNA 42206720 42210449 42206720 ID=MS.gene025386.t1;Parent=MS.gene025386
chr2.2 exon 42210325 42210449 42210325 ID=MS.gene025386.t1.exon1;Parent=MS.gene025386.t1
chr2.2 CDS 42210325 42210449 42210325 ID=cds.MS.gene025386.t1;Parent=MS.gene025386.t1
chr2.2 exon 42207413 42207518 42207413 ID=MS.gene025386.t1.exon2;Parent=MS.gene025386.t1
chr2.2 CDS 42207413 42207518 42207413 ID=cds.MS.gene025386.t1;Parent=MS.gene025386.t1
chr2.2 exon 42207122 42207314 42207122 ID=MS.gene025386.t1.exon3;Parent=MS.gene025386.t1
chr2.2 CDS 42207122 42207314 42207122 ID=cds.MS.gene025386.t1;Parent=MS.gene025386.t1
chr2.2 exon 42206944 42207001 42206944 ID=MS.gene025386.t1.exon4;Parent=MS.gene025386.t1
chr2.2 CDS 42206944 42207001 42206944 ID=cds.MS.gene025386.t1;Parent=MS.gene025386.t1
chr2.2 exon 42206720 42206867 42206720 ID=MS.gene025386.t1.exon5;Parent=MS.gene025386.t1
chr2.2 CDS 42206720 42206867 42206720 ID=cds.MS.gene025386.t1;Parent=MS.gene025386.t1
Gene Sequence

>MS.gene025386

ATGGATGGTGTTACTGTTAAGGTTGAGCCAGAGAAGTTTACTGTTCTAGAATCTTCTCCTGTTGCTGTTGATGGTGCTAGTTCCAACATGGTTGAGGGGTCAATGATGAATTCTTCAGCAAACAAGTAAGTGTTTTATATTTTCACCAAAAAAGCATGTTTTTTTTATTACTGTTGTTTTATGTGTTTCACTTTTTTTATGTTTATACAGCATGTTCCTTCAAGACCCTTTTTTTTTGTTTCCAATTTTTGTTTCTGTTTTGATGAATGATTACTCACTAACTTGGTTATTTTAATTTTGGTTGAATGTTTTTTTTTTTTTGGATGTCATAAAAACTGTAGATGAACAATGAACTAAGGTGTTTCTTTTCCAATTTTTGTACCTTAAATGGTTTCAATTTGTGTGAATTGATTTTAAATATACTTGAACTTCAGTTTTTTTGAATTAAAATTGTTCTAATGTGTTAAGGGAACTTGTTAATTATAATGATCTGAACTGATGTCATTTGACTCTACTAAAAACAAAATTTTCGGTTAAATTATTTATCCTATGGCCTTGTTTAATGGTTTCTCCTAAAGTTTTCTTTGATCTACTTTTCTTACTGGAGCTTTAATTGAAATTCTTTCAACATGATCAAACTACCCTACTTTTCTCCTCAACAATTGGTGTTTGTTTCGTATATATATTTGTTTCGTGTTTGTGTTGAGCATGGCTCATAGTCATTGATAAGTGGGAACCGCACGTAATATTTAAGTAGATCATAGTAGTTAAACAAAGTTTTGTACTCTGTAACCCTGATTCATTGCATAATAGAACGGTACAACAACCACTCGACTGTTAGAGATGTACGCGCAATTGGCCGAGCTCCGTAATTAATCGTTAAGTTCTTGTGTTTATCTTCTTACTTGAGAACCGGATTGCTATTGTAACTTCATTTTAGTCTGTTGGTGGAACCTTTCTACTGTACAATCAGATTATAGAGTATCATTGTTCTTATCTTAGTAGTTAAGAGTTGTTAAGGGATAGAATAAAGGTAAAATAAGAGCAGTTAAGAGTTGTTACTCGTTAGAGTAGTTAGTTAGCTGTTCGGTTAGGTAGAGAATACAATGATAGAGATGATCATTGAATTTTGTTTCTTTTGTGAATAGAGTAGAGACTCTATTGTGAAAGAGAACCCCTTGAAGAAGAGACTCCTACTCTTTTCTATTTTCACAATAAAATTCTTCTTTCTTTTCAATTCAACTCTTGGATTACTAACAATATCATAGGTCTTTTCTAAATCAATGAAGACAGTGTGGAAGTTTTCCTGTATTGTTCTGCATCTTTTCATCGAACCTTGGTATGTATCCTTCACGGTTGACCTTTCTGTCATAAGCAAAATTAATGCTGTTATATGCAAGTCAAATTTTTCGAACATTCTCTGAAATTATGCTTTTCATATTGTAAAATTAGATGAGCACCACCAATGTGCCGTGGCAGCTGCCATAGGCCACAACACCTATTATATCTGACATAGACCGCATCACCATGTTAATATGGTGGATTGTTGGCTTGCCACCATAATCTGCCATCTGCAGATGGATAACACGGGATATTTTGGTTTTTTGGCTTTTCTGGTTAGCATTAAAAATCAAATACACGATTTTTCTGAATTCTGAACCTTGGACTTAATACTTTTTCATTTTGCCAACTTTTTAAATAACCAAGATCATTGCTTGGCAAATATGATACGGACCTTTTTCGTCAAATTGAATATAAAGGAACTATAGTACAAACTCATCGTCTTGATTCAGAAATGAAGAACACAATTTTTTATTTGATTGAACAACAAAAGCATAGGAAACCATTGGGATTTAGTCTCTGCAATTGAATAAATCTACTGAAGACGTGTTTCAGCATACTGCATACCTCCCTGTTCTGTTTTGAATGAAATCATTGGAAGTCTCACATATAAAGATGTATCGTTGTTTTTGATGGCCAGCATAATTTTACTTTTCTGATCGGAGATATACTTTAACACAAATTTCAACTGCATAAGTTGATGAAATTCACGTCTAACCAAATCGAACACGGACAAAAGTTTCTGATGATAATCCACTGTAATTACTTATTCCATTACATATGTTTCAGCAAGATTCGGAGGCTGAATTCATGCATATTTCGGAGAACTGCAGTCAAATACTGTTGATGTGGCCTTAATAACAGGTTGTGTTGTGGTTTCAGCAACACAAAAACTGTTATATCACGGCCGCATCACAAACCTTTATTTTAAACTCTTAGCTACTATGAGTATTAAGACTTACCTAAAATGAAACAATATCTAGTGCTTTAGCTACTTGAATTCAAGACCATTCGCTGGTTAATCACATTTGAGTTCTATCTGATTGTTGTTAGTTTATGTTTATGTGTTTGCCTTTTGAAAATAGTTAACCGAATTGAAATATTTTATGCCACATTTTCTATGAGCTTCCACTGTTATACAATGATTTTGCTGGAGAAAGTGAAAGTATAAAAAATTGATATAAGGAATCATGCTTGACTTAATTCCACTATTTTGGTGTGTTTTGAAGGGCCAATTAACCACTGATACATATATTTTGGTTTATCAGAAATAACATCTTGAAGACTCCATGTACTGAGTTTTCTAGGCTTAACATCAAAATCAATCTTTGACATAACGCATCTCTTATTACTTCAAAGAACTTAGTATTCTTCAAAATTTAGCAAATATACGAAATATATGAGGTCTTGTACATCCATAAACTCTATTCTAGAAAACCAAAGTTACAATTTCCACCAATATCAGGCTTATCTATGCTTCTGTGTTTGTTTGAGTGGTTTATTTAGTTGTTTTTTTTCGTTATTTTTATTATCATCATTATTACTACTTTAGTCATCAAAAACTGGTTGCTTTGTAACTTCCTCTTTTAGGTCAATGCCAGCATCTGGAATGAATCCAGTGATTGCTAATACAACTCAGCTTACCATCTTCTATAATGGGAGTATTTGTATCTATGATGGAATTCCTGCAGAAAAGGTAGCCCTACGCTACACTGAGATGATTACTGAAACTTACAGCTCTATTTTCCATGTTGCGACACAATCAAAATCATATTAGACTCTATTTACTAACAGGTGCAAGAAATAATGCTCATTGCTGCTGCTGCTGCCAAGTCTAGTGAAACGAAGAAGATTGTGAAACAATCTCCCATTCCCGTTCCTTCACCTGTTCCTTCAAGGCCTTCTTCGCCACATGGAACTGCTGATAATATCACTTCACCGCAGGCACCTCCCTTTCCTGCAAAGAGTTCTATCTGCAGGCTGCAAGGTGTGAATTTTCCTATTAACTATGTCTTCAACTATTTAGTAACTGTTTGAACTTGAATAGACTCTTAATGTTACCATAATCTATATGTCTCGTGTGGTTTTTCTTGATATTTCTCTTCAGAATTTCCAATTGCACGCAGACATTCACTTCAAATGTTTCTTCAGAAGCGTCGAGACAGGTACTGTCTACCTCCCATGTTACCATAATCTATAGTTCTAGTTTTCTTACATGCATAATTTATGTTTGTATTACAGGTTGGGTAGTAAAGCTCCTTATCCTTCCTCGCCAAAAACAAAGGTAGCTGACAACATGGAGAACAACTTCGGTGCTGACAATTCGCCAGATTCGGTTTCAATGAAGGAACCAATGGAAGAATTTCAGCCAACTATATCTGCATCTTGA
Protein sequence

>MS.gene025386.t1

MDGVTVKVEPEKFTVLESSPVAVDGASSNMVEGSMMNSSANKSMPASGMNPVIANTTQLTIFYNGSICIYDGIPAEKVQEIMLIAAAAAKSSETKKIVKQSPIPVPSPVPSRPSSPHGTADNITSPQAPPFPAKSSICRLQEFPIARRHSLQMFLQKRRDRLGSKAPYPSSPKTKVADNMENNFGADNSPDSVSMKEPMEEFQPTISAS