AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85313

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85313.t1 MTR_2g044910 93.780 209 11 1 1 209 1 207 2.67e-141 393
MS.gene85313.t1 MTR_2g044910 93.373 166 9 1 44 209 1 164 8.03e-110 311
MS.gene85313.t1 MTR_2g044910 85.897 156 15 3 1 154 1 151 7.22e-88 256
MS.gene85313.t1 MTR_4g124360 58.209 134 48 3 38 168 25 153 1.28e-42 142
MS.gene85313.t1 MTR_4g124360 58.824 136 47 4 52 184 1 130 4.93e-40 134
MS.gene85313.t1 MTR_5g013515 36.066 122 63 3 57 176 13 121 4.22e-15 69.7
MS.gene85313.t1 MTR_2g042900 32.031 128 64 3 58 168 112 233 8.32e-14 68.6
MS.gene85313.t1 MTR_5g013530 33.333 114 59 3 58 171 22 118 1.17e-12 63.5
MS.gene85313.t1 MTR_4g124960 32.231 121 45 3 50 168 91 176 1.43e-12 64.3
MS.gene85313.t1 MTR_5g013530 33.333 114 59 3 58 171 44 140 3.76e-12 62.4
MS.gene85313.t1 MTR_4g124960 31.933 119 44 3 50 166 91 174 8.54e-12 61.6
MS.gene85313.t1 MTR_5g013520 31.092 119 65 3 58 176 15 116 6.41e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85313.t1 AT5G20900 53.448 116 46 4 58 171 57 166 7.89e-31 112
MS.gene85313.t1 AT3G43440 38.760 129 53 4 57 183 130 234 8.48e-16 73.6
MS.gene85313.t1 AT3G43440 36.923 130 56 4 56 183 43 148 1.67e-15 71.2
MS.gene85313.t1 AT1G74950 37.097 124 49 4 59 168 120 228 4.91e-13 66.2
MS.gene85313.t1 AT1G19180 35.043 117 62 3 54 168 56 160 1.23e-12 64.3
MS.gene85313.t1 AT1G19180 34.746 118 61 4 54 168 122 226 2.40e-12 64.3
MS.gene85313.t1 AT1G19180 34.746 118 61 4 54 168 122 226 2.40e-12 64.3

Find 46 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTACCATCTTCTATAA+TGG 0.263317 2.4:-45475651 MS.gene85313:CDS
TTCCTTCACCTGTTCCTTCA+AGG 0.273672 2.4:-45475401 MS.gene85313:CDS
TCCTTCATTGAAACCGAATC+TGG 0.348326 2.4:+45474950 None:intergenic
TTTAGGTCAATGCCAGCATT+TGG 0.374746 2.4:-45475702 MS.gene85313:intron
TGCTGACAATTCGCCAGATT+CGG 0.379407 2.4:-45474963 MS.gene85313:CDS
TCTTCTCCTATTGCTGTTGA+TGG 0.379691 2.4:-45478058 MS.gene85313:CDS
AGTGAATGTCTGCGTGCTAT+TGG 0.383673 2.4:+45475168 None:intergenic
TGAGTTGTATTAGCAATCAT+TGG 0.398568 2.4:+45475672 None:intergenic
AGCTTACCATCTTCTATAAT+GGG 0.398580 2.4:-45475650 MS.gene85313:CDS
CTGCAGATAGAACTCTTTGC+AGG 0.418330 2.4:+45475321 None:intergenic
GCCAGATTCGGTTTCAATGA+AGG 0.420770 2.4:-45474951 MS.gene85313:CDS
ATTGGATTCGTTCCAAATGC+TGG 0.440801 2.4:+45475690 None:intergenic
CTAGCACCATCAACAGCAAT+AGG 0.441302 2.4:+45478052 None:intergenic
GCTAGCTCCAACATGGTTGA+GGG 0.442261 2.4:-45478034 MS.gene85313:CDS
ACAGGTGAAGGAACAGGAAC+GGG 0.448799 2.4:+45475411 None:intergenic
TTCTTCAGAAGCGGCGAGAC+AGG 0.453708 2.4:-45475137 MS.gene85313:intron
GTTGGCTGAAATTCTTCCAT+TGG 0.460471 2.4:+45474926 None:intergenic
CTTCTTCGTTTCACTAGACT+TGG 0.471119 2.4:+45475449 None:intergenic
TGCTAGCTCCAACATGGTTG+AGG 0.475742 2.4:-45478035 MS.gene85313:CDS
TGATGGAATTCCTGCAGAAA+AGG 0.477638 2.4:-45475613 MS.gene85313:intron
GGGAGTATTTGTATCTATGA+TGG 0.478209 2.4:-45475630 MS.gene85313:CDS
GCAGTTCCATGTGGCGAAGA+AGG 0.484372 2.4:+45475378 None:intergenic
CTGCAAAGAGTTCTATCTGC+AGG 0.484476 2.4:-45475320 MS.gene85313:CDS
TCTAGAACAGTAAACTTCTC+TGG 0.487430 2.4:+45478082 None:intergenic
GAAGGAACAGGTGAAGGAAC+AGG 0.487448 2.4:+45475405 None:intergenic
TGTGGCGAAGAAGGCCTTGA+AGG 0.491077 2.4:+45475387 None:intergenic
ATAGAACTCTTTGCAGGAAA+GGG 0.495560 2.4:+45475327 None:intergenic
TTCAAATGTTTCTTCAGAAG+CGG 0.502165 2.4:-45475146 MS.gene85313:CDS
AATACTCCCATTATAGAAGA+TGG 0.513403 2.4:+45475644 None:intergenic
GAAGAAGGCCTTGAAGGAAC+AGG 0.524051 2.4:+45475393 None:intergenic
GCTCAAGATGCAGATATAGT+TGG 0.526009 2.4:+45474908 None:intergenic
TCAAGGCCTTCTTCGCCACA+TGG 0.540794 2.4:-45475384 MS.gene85313:CDS
AGTTCTATCTGCAGGCTGCA+AGG 0.555717 2.4:-45475312 MS.gene85313:intron
GATAGAACTCTTTGCAGGAA+AGG 0.557564 2.4:+45475326 None:intergenic
CATTGACCCCTCAACCATGT+TGG 0.571586 2.4:+45478027 None:intergenic
TGATAATATCGCTTCACCAC+AGG 0.571663 2.4:-45475355 MS.gene85313:CDS
GGCCTTGAAGGAACAGGTGA+AGG 0.574210 2.4:+45475399 None:intergenic
GGTTTCAATGAAGGAACCAA+TGG 0.581761 2.4:-45474942 MS.gene85313:CDS
AACAGGTGAAGGAACAGGAA+CGG 0.583312 2.4:+45475410 None:intergenic
AACAAAGGTAGCTGACAACA+TGG 0.591184 2.4:-45474999 MS.gene85313:CDS
TGATGGTGCTAGCTCCAACA+TGG 0.603125 2.4:-45478041 MS.gene85313:CDS
GACAACATGGAGAACAACTT+CGG 0.616517 2.4:-45474986 MS.gene85313:CDS
GAACTCTTTGCAGGAAAGGG+AGG 0.634573 2.4:+45475330 None:intergenic
GCAGGAAAGGGAGGTGCCTG+TGG 0.640323 2.4:+45475339 None:intergenic
CTAGCTCCAACATGGTTGAG+GGG 0.656060 2.4:-45478033 MS.gene85313:CDS
ATATTATCAGCAGTTCCATG+TGG 0.739266 2.4:+45475369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTAGTTATTTAAAAAGT+TGG + chr2.4:45476555-45476574 None:intergenic 15.0%
!!! ATAAAAAACATGCTTTTTTT+TGG + chr2.4:45475058-45475077 None:intergenic 15.0%
!!! ATTTTAACTCAAAAAACTAA+AGG + chr2.4:45475329-45475348 None:intergenic 15.0%
!! ATAATTTATGTTTGTACTAC+AGG - chr2.4:45477962-45477981 MS.gene85313:intron 20.0%
!! ATACACTGATACATATATTT+AGG - chr2.4:45477059-45477078 MS.gene85313:intron 20.0%
!! ATATGAAAATCGAAAAGATT+AGG + chr2.4:45476056-45476075 None:intergenic 20.0%
!! CAAAACAGAAACAAAAAAAA+GGG + chr2.4:45475140-45475159 None:intergenic 20.0%
!! TAATTCATTGCATAATAGAA+CGG - chr2.4:45475690-45475709 MS.gene85313:CDS 20.0%
!! TCAAAACAGAAACAAAAAAA+AGG + chr2.4:45475141-45475160 None:intergenic 20.0%
!! TTCTATTATGCAATGAATTA+GGG + chr2.4:45475690-45475709 None:intergenic 20.0%
!!! ACTATGAAAAACGAAATTTT+CGG - chr2.4:45475409-45475428 MS.gene85313:CDS 20.0%
!!! ACTGTATTATTTTCTCTTTT+AGG - chr2.4:45477300-45477319 MS.gene85313:intron 20.0%
!!! ATACAAATTGATACCATTTT+AGG + chr2.4:45475281-45475300 None:intergenic 20.0%
!!! TAGCTTGGTTATTTTAATTT+TGG - chr2.4:45475176-45475195 MS.gene85313:CDS 20.0%
! AAGAACTCAACGATTAATTA+TGG + chr2.4:45475764-45475783 None:intergenic 25.0%
! AATGGAATAAGTCATTTGAA+TGG + chr2.4:45476858-45476877 None:intergenic 25.0%
! ACAAACAATTGATGTTTGTA+TGG - chr2.4:45475557-45475576 MS.gene85313:intron 25.0%
! ACATGAATTTCATCAACTTA+TGG + chr2.4:45476793-45476812 None:intergenic 25.0%
! AGTGTATATGCTACATTAAT+GGG + chr2.4:45477046-45477065 None:intergenic 25.0%
! GTTCTATTATGCAATGAATT+AGG + chr2.4:45475691-45475710 None:intergenic 25.0%
! GTTCTTATCTTAGTAGTTAA+GGG - chr2.4:45475860-45475879 MS.gene85313:intron 25.0%
! TAGTTAAGGGATAGAATAAA+GGG - chr2.4:45475873-45475892 MS.gene85313:intron 25.0%
! TGTTCTTATCTTAGTAGTTA+AGG - chr2.4:45475859-45475878 MS.gene85313:intron 25.0%
! TTAACTCTCTATATACTAAC+AGG - chr2.4:45477514-45477533 MS.gene85313:intron 25.0%
! TTATGAGAAACCATTAAACA+AGG + chr2.4:45475455-45475474 None:intergenic 25.0%
!! CATACACAAAATTTGAAACA+TGG + chr2.4:45477921-45477940 None:intergenic 25.0%
!! TTCCAATTTTGTACCTAAAA+TGG - chr2.4:45475265-45475284 MS.gene85313:intron 25.0%
!! TTTCTTTTCAATTCAACTCT+TGG - chr2.4:45476086-45476105 MS.gene85313:intron 25.0%
!!! AGATATGTCATTGTTTTTCA+TGG - chr2.4:45476710-45476729 MS.gene85313:intron 25.0%
!!! ATGAAAAAGTATTAAGTCCA+AGG + chr2.4:45476528-45476547 None:intergenic 25.0%
!!! TACCATTTTAGGTACAAAAT+TGG + chr2.4:45475270-45475289 None:intergenic 25.0%
!!! TGTCTTCATTGATTTAGAAA+AGG + chr2.4:45476132-45476151 None:intergenic 25.0%
!!! TTTCGTTTTTCATAGTCAAA+TGG + chr2.4:45475405-45475424 None:intergenic 25.0%
AAAATTGTTCTGATGAGTTG+AGG - chr2.4:45475344-45475363 MS.gene85313:CDS 30.0%
AACAAAAAAAAGGGTCTTGA+AGG + chr2.4:45475131-45475150 None:intergenic 30.0%
AACAATCCACCATATAAACA+TGG + chr2.4:45476394-45476413 None:intergenic 30.0%
AATACTCCCATTATAGAAGA+TGG + chr2.4:45477378-45477397 None:intergenic 30.0%
AGCTTACCATCTTCTATAAT+GGG - chr2.4:45477369-45477388 MS.gene85313:intron 30.0%
CACACGAGACATATAAATTA+TGG + chr2.4:45477802-45477821 None:intergenic 30.0%
CAGTGTATATGCTACATTAA+TGG + chr2.4:45477047-45477066 None:intergenic 30.0%
CATAATTTATATGTCTCGTG+TGG - chr2.4:45477800-45477819 MS.gene85313:intron 30.0%
CGGTTAAATTGTTTATCCTA+TGG - chr2.4:45475429-45475448 MS.gene85313:CDS 30.0%
GAAAGGTTACAATAGAAATC+TGG + chr2.4:45475810-45475829 None:intergenic 30.0%
GTAGTTAAGGGATAGAATAA+AGG - chr2.4:45475872-45475891 MS.gene85313:intron 30.0%
GTGTATAACCAAATTGAACA+GGG - chr2.4:45476811-45476830 MS.gene85313:intron 30.0%
GTGTATATGCTACATTAATG+GGG + chr2.4:45477045-45477064 None:intergenic 30.0%
TCTCGCTGAAAAATATGTAA+TGG + chr2.4:45476876-45476895 None:intergenic 30.0%
TCTGTTCTGTATGAAATCAT+TGG - chr2.4:45476672-45476691 MS.gene85313:intron 30.0%
TGAATAATTACTCACTAGCT+TGG - chr2.4:45475161-45475180 MS.gene85313:CDS 30.0%
TGAGTTGTATTAGCAATCAT+TGG + chr2.4:45477350-45477369 None:intergenic 30.0%
TGGTCTATGTCAGATATAAT+AGG + chr2.4:45476366-45476385 None:intergenic 30.0%
TGTGTATAACCAAATTGAAC+AGG - chr2.4:45476810-45476829 MS.gene85313:intron 30.0%
TTATGTTTGTACTACAGGTT+GGG - chr2.4:45477967-45477986 MS.gene85313:intron 30.0%
TTCAAAGAACTTAGTCATCA+AGG - chr2.4:45477174-45477193 MS.gene85313:intron 30.0%
TTCAAATGTTTCTTCAGAAG+CGG - chr2.4:45477873-45477892 MS.gene85313:intron 30.0%
TTTATGTTTGTACTACAGGT+TGG - chr2.4:45477966-45477985 MS.gene85313:intron 30.0%
! AAATTGTTCTGATGAGTTGA+GGG - chr2.4:45475345-45475364 MS.gene85313:CDS 30.0%
! ATAGATAACAGGGGATATTT+TGG - chr2.4:45476443-45476462 MS.gene85313:intron 30.0%
! CAAACATCAATTGTTTGTTG+AGG + chr2.4:45475555-45475574 None:intergenic 30.0%
! GCTTTTTGTGTTTGTTTGAA+TGG - chr2.4:45477206-45477225 MS.gene85313:intron 30.0%
! GTTGCAGATTTTTATTGAAC+TGG + chr2.4:45477020-45477039 None:intergenic 30.0%
! TACATCTTTTCATTGAACCT+TGG - chr2.4:45476177-45476196 MS.gene85313:intron 30.0%
! TGATTGAACAACAAAAGCAT+AGG - chr2.4:45476609-45476628 MS.gene85313:intron 30.0%
! TTTTTCTGAATTCTGAACCT+TGG - chr2.4:45476508-45476527 MS.gene85313:intron 30.0%
!! ATGTTTATATGGTGGATTGT+TGG - chr2.4:45476393-45476412 MS.gene85313:intron 30.0%
!! TATTTTTCAGCGAGATTTTG+AGG - chr2.4:45476881-45476900 MS.gene85313:intron 30.0%
!!! TTTTGGTTTTTTGGCTTTTC+TGG - chr2.4:45476460-45476479 MS.gene85313:intron 30.0%
AAAAGGCTTGTGACAATGTT+AGG + chr2.4:45476115-45476134 None:intergenic 35.0%
AAACCATTAAACAAGGCCAT+AGG + chr2.4:45475448-45475467 None:intergenic 35.0%
AAATACTACGAGCCACTTAT+CGG + chr2.4:45475626-45475645 None:intergenic 35.0%
ACATCACCATGTTTATATGG+TGG - chr2.4:45476385-45476404 MS.gene85313:intron 35.0%
ACCACATCACCATGTTTATA+TGG - chr2.4:45476382-45476401 MS.gene85313:intron 35.0%
AGAGTAGTTAGTTAGCTGTT+TGG - chr2.4:45475928-45475947 MS.gene85313:intron 35.0%
AGGCTTCTTCATGCATATTA+TGG - chr2.4:45476901-45476920 MS.gene85313:intron 35.0%
AGTCATGACTCTATTGTGAA+AGG - chr2.4:45476009-45476028 MS.gene85313:intron 35.0%
AGTTAGTTAGCTGTTTGGTT+GGG - chr2.4:45475933-45475952 MS.gene85313:intron 35.0%
ATAGAACTCTTTGCAGGAAA+GGG + chr2.4:45477695-45477714 None:intergenic 35.0%
ATATTATCAGCAGTTCCATG+TGG + chr2.4:45477653-45477672 None:intergenic 35.0%
ATTAGGAGTCTCTTCTTCAA+GGG + chr2.4:45476039-45476058 None:intergenic 35.0%
ATTTCGATTGTGTCGTAACA+TGG + chr2.4:45477489-45477508 None:intergenic 35.0%
CAACGATTAATTATGGAGCT+CGG + chr2.4:45475757-45475776 None:intergenic 35.0%
CAGCTTACCATCTTCTATAA+TGG - chr2.4:45477368-45477387 MS.gene85313:intron 35.0%
CATCTGATTGTACAGTAGAA+AGG + chr2.4:45475827-45475846 None:intergenic 35.0%
GTCATGACTCTATTGTGAAA+GGG - chr2.4:45476010-45476029 MS.gene85313:intron 35.0%
TAATTTCGCTTATGACAGAG+AGG + chr2.4:45476223-45476242 None:intergenic 35.0%
TAGTCGAAGATATGACTAGT+AGG + chr2.4:45477743-45477762 None:intergenic 35.0%
TAGTTAGTTAGCTGTTTGGT+TGG - chr2.4:45475932-45475951 MS.gene85313:intron 35.0%
TGAAACACTGGAAATCTCAA+TGG + chr2.4:45476638-45476657 None:intergenic 35.0%
TGTTGAGTTTGTGTTGAGTA+TGG - chr2.4:45475590-45475609 MS.gene85313:intron 35.0%
! ACCATATAAACATGGTGATG+TGG + chr2.4:45476386-45476405 None:intergenic 35.0%
! ACTTTTGTCCCTGTTCAATT+TGG + chr2.4:45476822-45476841 None:intergenic 35.0%
! CACAAAATTTGAAACATGGC+AGG + chr2.4:45477917-45477936 None:intergenic 35.0%
! GTCAGATATAATAGGTGTTG+TGG + chr2.4:45476358-45476377 None:intergenic 35.0%
! TCTAGAACAGTAAACTTCTC+TGG + chr2.4:45474940-45474959 None:intergenic 35.0%
!! GGGAGTATTTGTATCTATGA+TGG - chr2.4:45477389-45477408 MS.gene85313:intron 35.0%
!! TATCCTATGGCCTTGTTTAA+TGG - chr2.4:45475442-45475461 MS.gene85313:CDS 35.0%
!! TTCCACGTTTGTGTTTTGAA+GGG - chr2.4:45476994-45477013 MS.gene85313:intron 35.0%
!! TTTCCACGTTTGTGTTTTGA+AGG - chr2.4:45476993-45477012 MS.gene85313:intron 35.0%
!!! CAGGGGATATTTTGGTTTTT+TGG - chr2.4:45476451-45476470 MS.gene85313:intron 35.0%
!!! TTGTCAGCTACCTTTGTTTT+TGG + chr2.4:45478018-45478037 None:intergenic 35.0%
AACAAAGGTAGCTGACAACA+TGG - chr2.4:45478020-45478039 MS.gene85313:CDS 40.0%
ACCTTGGTATGTATCCTTCA+CGG - chr2.4:45476193-45476212 MS.gene85313:intron 40.0%
AGCCTAGAAAACTCAGTACA+AGG + chr2.4:45477112-45477131 None:intergenic 40.0%
ATGCTACACAACCGTGATTA+AGG + chr2.4:45476956-45476975 None:intergenic 40.0%
ATTGGATTCGTTCCAAATGC+TGG + chr2.4:45477332-45477351 None:intergenic 40.0%
CATCTGCAGATAGATAACAG+GGG - chr2.4:45476434-45476453 MS.gene85313:intron 40.0%
CCATCTGCAGATAGATAACA+GGG - chr2.4:45476433-45476452 MS.gene85313:intron 40.0%
CTTCTTCGTTTCACTAGACT+TGG + chr2.4:45477573-45477592 None:intergenic 40.0%
GACAACATGGAGAACAACTT+CGG - chr2.4:45478033-45478052 MS.gene85313:CDS 40.0%
GATAGAACTCTTTGCAGGAA+AGG + chr2.4:45477696-45477715 None:intergenic 40.0%
GATTAGGAGTCTCTTCTTCA+AGG + chr2.4:45476040-45476059 None:intergenic 40.0%
GCAGTCAAAAACTGTTGATG+CGG - chr2.4:45476930-45476949 MS.gene85313:intron 40.0%
GGTTTCAATGAAGGAACCAA+TGG - chr2.4:45478077-45478096 MS.gene85313:CDS 40.0%
GTTAGAGATGTACACGCAAT+TGG - chr2.4:45475731-45475750 MS.gene85313:intron 40.0%
GTTGGCTGAAATTCTTCCAT+TGG + chr2.4:45478096-45478115 None:intergenic 40.0%
TATCTGCAGATGGCAGATTA+TGG + chr2.4:45476426-45476445 None:intergenic 40.0%
TCATACAGAACAGAACAGAG+AGG + chr2.4:45476666-45476685 None:intergenic 40.0%
TCCTTCATTGAAACCGAATC+TGG + chr2.4:45478072-45478091 None:intergenic 40.0%
TGATAATATCGCTTCACCAC+AGG - chr2.4:45477664-45477683 MS.gene85313:intron 40.0%
TGATGGAATTCCTGCAGAAA+AGG - chr2.4:45477406-45477425 MS.gene85313:intron 40.0%
TTTAGGTCAATGCCAGCATT+TGG - chr2.4:45477317-45477336 MS.gene85313:intron 40.0%
! ATAATAGGTGTTGTGGCCTA+TGG + chr2.4:45476351-45476370 None:intergenic 40.0%
! CTCCTTGTACTGAGTTTTCT+AGG - chr2.4:45477107-45477126 MS.gene85313:intron 40.0%
! GTTTGTTGAGGAGAAAAGCA+GGG + chr2.4:45475543-45475562 None:intergenic 40.0%
! TCTTCTCCTATTGCTGTTGA+TGG - chr2.4:45474961-45474980 MS.gene85313:CDS 40.0%
! TGTTTGTTGAGGAGAAAAGC+AGG + chr2.4:45475544-45475563 None:intergenic 40.0%
AACAGGTGAAGGAACAGGAA+CGG + chr2.4:45477612-45477631 None:intergenic 45.0%
ACCGTGAAGGATACATACCA+AGG + chr2.4:45476197-45476216 None:intergenic 45.0%
AGTGAATGTCTGCGTGCTAT+TGG + chr2.4:45477854-45477873 None:intergenic 45.0%
CCCTGTTATCTATCTGCAGA+TGG + chr2.4:45476436-45476455 None:intergenic 45.0%
CTAGCACCATCAACAGCAAT+AGG + chr2.4:45474970-45474989 None:intergenic 45.0%
CTGCAAAGAGTTCTATCTGC+AGG - chr2.4:45477699-45477718 MS.gene85313:intron 45.0%
CTGCAGATAGAACTCTTTGC+AGG + chr2.4:45477701-45477720 None:intergenic 45.0%
GCCATCTGCAGATAGATAAC+AGG - chr2.4:45476432-45476451 MS.gene85313:intron 45.0%
TCCTTCCTCGCCAAAAACAA+AGG - chr2.4:45478005-45478024 MS.gene85313:intron 45.0%
TGCTGACAATTCGCCAGATT+CGG - chr2.4:45478056-45478075 MS.gene85313:CDS 45.0%
TGTTGATGCGGCCTTAATCA+CGG - chr2.4:45476942-45476961 MS.gene85313:intron 45.0%
TTCCTTCACCTGTTCCTTCA+AGG - chr2.4:45477618-45477637 MS.gene85313:intron 45.0%
! AGAGAGGTATGCTGAAACAC+TGG + chr2.4:45476650-45476669 None:intergenic 45.0%
! GCCAGATTCGGTTTCAATGA+AGG - chr2.4:45478068-45478087 MS.gene85313:CDS 45.0%
!!! ACCTTTGTTTTTGGCGAGGA+AGG + chr2.4:45478009-45478028 None:intergenic 45.0%
!!! AGCTACCTTTGTTTTTGGCG+AGG + chr2.4:45478013-45478032 None:intergenic 45.0%
!!! GTTTTTGGCGAGGAAGGATA+GGG + chr2.4:45478003-45478022 None:intergenic 45.0%
!!! TGTTTTTGGCGAGGAAGGAT+AGG + chr2.4:45478004-45478023 None:intergenic 45.0%
ACAGGTGAAGGAACAGGAAC+GGG + chr2.4:45477611-45477630 None:intergenic 50.0%
AGTTCTATCTGCAGGCTGCA+AGG - chr2.4:45477707-45477726 MS.gene85313:intron 50.0%
CATTGACCCCTCAACCATGT+TGG + chr2.4:45474995-45475014 None:intergenic 50.0%
CTAGCTCCAACATGGTTGAG+GGG - chr2.4:45474986-45475005 MS.gene85313:CDS 50.0%
CTGCAGATGGCAGATTATGG+TGG + chr2.4:45476423-45476442 None:intergenic 50.0%
GAACTCTTTGCAGGAAAGGG+AGG + chr2.4:45477692-45477711 None:intergenic 50.0%
GAAGAAGGCCTTGAAGGAAC+AGG + chr2.4:45477629-45477648 None:intergenic 50.0%
GAAGGAACAGGTGAAGGAAC+AGG + chr2.4:45477617-45477636 None:intergenic 50.0%
GCTAGCTCCAACATGGTTGA+GGG - chr2.4:45474985-45475004 MS.gene85313:CDS 50.0%
GGCCCTTCAAAACACAAACG+TGG + chr2.4:45476999-45477018 None:intergenic 50.0%
GGCTCATTGTCACCGATAAG+TGG - chr2.4:45475611-45475630 MS.gene85313:intron 50.0%
TGCTAGCTCCAACATGGTTG+AGG - chr2.4:45474984-45475003 MS.gene85313:CDS 50.0%
TGTTGAGGAGAAAAGCAGGG+AGG + chr2.4:45475540-45475559 None:intergenic 50.0%
! TGATGGTGCTAGCTCCAACA+TGG - chr2.4:45474978-45474997 MS.gene85313:CDS 50.0%
CAGTGTAGCGTAGCTGCGTA+GGG + chr2.4:45477435-45477454 None:intergenic 55.0%
GACAGAGAGGTCAACCGTGA+AGG + chr2.4:45476210-45476229 None:intergenic 55.0%
GCAGTTCCATGTGGCGAAGA+AGG + chr2.4:45477644-45477663 None:intergenic 55.0%
GGCCTTGAAGGAACAGGTGA+AGG + chr2.4:45477623-45477642 None:intergenic 55.0%
TCAAGGCCTTCTTCGCCACA+TGG - chr2.4:45477635-45477654 MS.gene85313:intron 55.0%
TCAGTGTAGCGTAGCTGCGT+AGG + chr2.4:45477436-45477455 None:intergenic 55.0%
TGTGGCGAAGAAGGCCTTGA+AGG + chr2.4:45477635-45477654 None:intergenic 55.0%
TTCTTCAGAAGCGGCGAGAC+AGG - chr2.4:45477882-45477901 MS.gene85313:intron 55.0%
! CGTAGGGCTACCTTTTCTGC+AGG + chr2.4:45477419-45477438 None:intergenic 55.0%
CAACTGCCACGGCACATTGG+TGG + chr2.4:45476328-45476347 None:intergenic 60.0%
GTGGCCTATGGCAACTGCCA+CGG + chr2.4:45476339-45476358 None:intergenic 60.0%
TGAGCACCACCAATGTGCCG+TGG - chr2.4:45476319-45476338 MS.gene85313:intron 60.0%
TGGCAACTGCCACGGCACAT+TGG + chr2.4:45476331-45476350 None:intergenic 60.0%
TGTGCCGTGGCAGTTGCCAT+AGG - chr2.4:45476332-45476351 MS.gene85313:intron 60.0%
!! GCAGGAAAGGGAGGTGCCTG+TGG + chr2.4:45477683-45477702 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 45474910 45478131 45474910 ID=MS.gene85313
chr2.4 mRNA 45474910 45478131 45474910 ID=MS.gene85313.t1;Parent=MS.gene85313
chr2.4 exon 45478007 45478131 45478007 ID=MS.gene85313.t1.exon1;Parent=MS.gene85313.t1
chr2.4 CDS 45478007 45478131 45478007 ID=cds.MS.gene85313.t1;Parent=MS.gene85313.t1
chr2.4 exon 45475614 45475719 45475614 ID=MS.gene85313.t1.exon2;Parent=MS.gene85313.t1
chr2.4 CDS 45475614 45475719 45475614 ID=cds.MS.gene85313.t1;Parent=MS.gene85313.t1
chr2.4 exon 45475313 45475505 45475313 ID=MS.gene85313.t1.exon3;Parent=MS.gene85313.t1
chr2.4 CDS 45475313 45475505 45475313 ID=cds.MS.gene85313.t1;Parent=MS.gene85313.t1
chr2.4 exon 45475138 45475195 45475138 ID=MS.gene85313.t1.exon4;Parent=MS.gene85313.t1
chr2.4 CDS 45475138 45475195 45475138 ID=cds.MS.gene85313.t1;Parent=MS.gene85313.t1
chr2.4 exon 45474910 45475057 45474910 ID=MS.gene85313.t1.exon5;Parent=MS.gene85313.t1
chr2.4 CDS 45474910 45475057 45474910 ID=cds.MS.gene85313.t1;Parent=MS.gene85313.t1
Gene Sequence

>MS.gene85313

ATGGATGGTGTTACTGTTAAGCTTGAGCCAGAGAAGTTTACTGTTCTAGAATCTTCTCCTATTGCTGTTGATGGTGCTAGCTCCAACATGGTTGAGGGGTCAATGATGAATTCTTCAGCAAACAAGTAAGTGTTTGATATTTTCACCAAAAAAAAGCATGTTTTTTATTACTGTTGTTTTATGTGTTTCACTTTTTTTATGTTTATACAACATATGTTCCTTCAAGACCCTTTTTTTTGTTTCTGTTTTGATGAATAATTACTCACTAGCTTGGTTATTTTAATTTTGGTTGAGTTTTGTTTTTTTTTTTTTAGATGTCATAAAAACTGTAAATGAACAATGAACTGTGTTTCTTTTCCAATTTTGTACCTAAAATGGTATCAATTTGTATGAGATGAGTTCAAATATACTTGTAACCTTTAGTTTTTTGAGTTAAAATTGTTCTGATGAGTTGAGGGAACTTGTTAATTATAATGATCTTCTGAACTGATGCCATTTGACTATGAAAAACGAAATTTTCGGTTAAATTGTTTATCCTATGGCCTTGTTTAATGGTTTCTCATAAAGTTTTCTTTGATCTACTTTTCTTACTAGAATCTTAATTGATATTCTTTCAACATGATCAAACCTCCCTGCTTTTCTCCTCAACAAACAATTGATGTTTGTATGGTATATATATTTGTTGAGTTTGTGTTGAGTATGGCTCATTGTCACCGATAAGTGGCTCGTAGTATTTAAGTAGATCATAGTATTTGAACAAAGCTTTGTACTCTGTAACCCTAATTCATTGCATAATAGAACGGTACAACAACTGCTCAACTGTTAGAGATGTACACGCAATTGGCCGAGCTCCATAATTAATCGTTGAGTTCTTGTGTTTATCTTCTTACGTGAGAACCAGATTTCTATTGTAACCTTTCTACTGTACAATCAGATGATAGAGTATCATTGTTCTTATCTTAGTAGTTAAGGGATAGAATAAAGGGAAAAGAAGAGTAGTTAAGAGTTGTTACTTGTTAGAGTAGTTAGTTAGCTGTTTGGTTGGGTAGAGAATACAATGATAGAGATGATCATTGAATATTATTTCTTATGTGAATAAAGTCATGACTCTATTGTGAAAGGGAAACCCTTGAAGAAGAGACTCCTAATCTTTTCGATTTTCATATAAAATTCTTCTTTCTTTTCAATTCAACTCTTGGATTCCTAACATTGTCACAAGCCTTTTCTAAATCAATGAAGACAATGTGTAAGTTTTCATGTATTGTTTTACATCTTTTCATTGAACCTTGGTATGTATCCTTCACGGTTGACCTCTCTGTCATAAGCGAAATTAATGCTGTTATATCAATATGCAAGTCAAATTTTTCGAACATTCTCTCAAATTACGCTTTTCATATTGTAAAATTAGATGAGCACCACCAATGTGCCGTGGCAGTTGCCATAGGCCACAACACCTATTATATCTGACATAGACCACATCACCATGTTTATATGGTGGATTGTTGGCTTGCCACCATAATCTGCCATCTGCAGATAGATAACAGGGGATATTTTGGTTTTTTGGCTTTTCTGGTTAGCATTAAAAATCAAATACACGATTTTTCTGAATTCTGAACCTTGGACTTAATACTTTTTCATTTCACCAACTTTTTAAATAACTAAGATCTTTGCTTCAGAAATGAAGACACGATTTTTTATTTGATTGAACAACAAAAGCATAGGAAGCCATTGAGATTTCCAGTGTTTCAGCATACCTCTCTGTTCTGTTCTGTATGAAATCATTGGAAGTCTCACATGTAAAGATATGTCATTGTTTTTCATGGCAGTATAGTTTTACTTTGCTGTTTGTAGACAAACTTTAACACAGAAAAACTTTTAAGCCATAAGTTGATGAAATTCATGTGTATAACCAAATTGAACAGGGACAAAAGTTTATGATGATAATCCATTCAAATGACTTATTCCATTACATATTTTTCAGCGAGATTTTGAGGCTTCTTCATGCATATTATGGAGAATTGCAGTCAAAAACTGTTGATGCGGCCTTAATCACGGTTGTGTAGCATTAAAAACTGTTTGAGCTTTTCCACGTTTGTGTTTTGAAGGGCCAGTTCAATAAAAATCTGCAACTTCCCCATTAATGTAGCATATACACTGATACATATATTTAGGTTTATCAGAAATAACATCTTGAAGACTCCTTGTACTGAGTTTTCTAGGCTTGACTTCAAAATCAATTTTTGACATAACTCATCTCTTATTACTTCAAAGAACTTAGTCATCAAGGCTTATCTATGCTTTTTGTGTTTGTTTGAATGGTTTTTAATTGTTGTTTTTTTCGTTATTTTTATTATTATCATTATTACTATTTTAGTAATAAAAAACTGATTACTGTATTATTTTCTCTTTTAGGTCAATGCCAGCATTTGGAACGAATCCAATGATTGCTAATACAACTCAGCTTACCATCTTCTATAATGGGAGTATTTGTATCTATGATGGAATTCCTGCAGAAAAGGTAGCCCTACGCAGCTACGCTACACTGAGATGATTAGTGAAACTTACAGCTCTATTTTCCATGTTACGACACAATCGAAATCATATTTAACTCTCTATATACTAACAGGTGCAAGAAATAATGCGCATTGCTGCTGCTGCTGCCAAGTCTAGTGAAACGAAGAAGATTGTGAAACAATCTCCCGTTCCTGTTCCTTCACCTGTTCCTTCAAGGCCTTCTTCGCCACATGGAACTGCTGATAATATCGCTTCACCACAGGCACCTCCCTTTCCTGCAAAGAGTTCTATCTGCAGGCTGCAAGGTGTGAATTCTCCTACTAGTCATATCTTCGACTATTTAGTTGTTTGAACTTGAATAGTCACTTAATGTCACCATAATTTATATGTCTCGTGTGGTTTTTCTTGATATTTCTCTTCAGAATTTCCAATAGCACGCAGACATTCACTTCAAATGTTTCTTCAGAAGCGGCGAGACAGGTACTGTCTACCTGCCATGTTTCAAATTTTGTGTATGACTACTAGTTTTCTTATTTGCATAATTTATGTTTGTACTACAGGTTGGGTAGTAAAGCTCCCTATCCTTCCTCGCCAAAAACAAAGGTAGCTGACAACATGGAGAACAACTTCGGTGCTGACAATTCGCCAGATTCGGTTTCAATGAAGGAACCAATGGAAGAATTTCAGCCAACTATATCTGCATCTTGA
Protein sequence

>MS.gene85313.t1

MDGVTVKLEPEKFTVLESSPIAVDGASSNMVEGSMMNSSANKSMPAFGTNPMIANTTQLTIFYNGSICIYDGIPAEKVQEIMRIAAAAAKSSETKKIVKQSPVPVPSPVPSRPSSPHGTADNIASPQAPPFPAKSSICRLQEFPIARRHSLQMFLQKRRDRLGSKAPYPSSPKTKVADNMENNFGADNSPDSVSMKEPMEEFQPTISAS