AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028204.t1 MTR_1g112270 45.669 254 133 4 30 280 47 298 1.46e-72 238
MS.gene028204.t1 MTR_8g017210 64.103 156 54 2 27 182 62 215 6.63e-72 235
MS.gene028204.t1 MTR_2g099990 63.462 156 55 2 27 182 46 199 4.35e-71 231
MS.gene028204.t1 MTR_2g093960 62.179 156 58 1 27 182 32 186 6.11e-71 229
MS.gene028204.t1 MTR_1g016010 61.392 158 60 1 27 184 41 197 1.52e-70 229
MS.gene028204.t1 MTR_1g112270 45.528 246 129 4 38 280 66 309 2.78e-69 229
MS.gene028204.t1 MTR_1g094115 60.897 156 59 2 27 182 47 200 4.78e-69 228
MS.gene028204.t1 MTR_4g059870 60.256 156 61 1 27 182 38 192 5.76e-69 226
MS.gene028204.t1 MTR_2g090745 62.162 148 54 2 35 182 15 160 1.77e-63 210
MS.gene028204.t1 MTR_2g099990 63.636 132 46 2 51 182 1 130 8.33e-59 197
MS.gene028204.t1 MTR_1g094115 53.205 156 54 3 27 182 47 183 2.18e-54 189
MS.gene028204.t1 MTR_2g017720 54.887 133 53 2 20 152 306 431 2.33e-39 146
MS.gene028204.t1 MTR_3g102000 46.565 131 58 5 27 150 6 131 1.90e-26 106
MS.gene028204.t1 MTR_5g030560 64.444 45 16 0 37 81 16 60 2.42e-16 73.9
MS.gene028204.t1 MTR_4g103250 34.884 86 56 0 311 396 58 143 1.10e-14 71.2
MS.gene028204.t1 MTR_1g093095 27.374 179 114 6 15 181 74 248 2.94e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028204.t1 AT2G01940 57.482 421 151 9 17 421 34 442 3.58e-164 469
MS.gene028204.t1 AT2G01940 56.738 423 152 11 17 421 34 443 1.04e-158 455
MS.gene028204.t1 AT1G68130 55.961 411 136 9 21 419 34 411 1.15e-149 431
MS.gene028204.t1 AT1G25250 53.846 429 137 10 3 421 8 385 2.54e-145 419
MS.gene028204.t1 AT1G25250 54.315 394 120 9 37 421 20 362 1.24e-134 391
MS.gene028204.t1 AT2G01940 54.247 365 139 9 73 421 1 353 1.10e-126 370
MS.gene028204.t1 AT1G68130 51.117 358 131 8 73 419 1 325 6.57e-113 334
MS.gene028204.t1 AT1G25250 50.000 358 119 9 73 421 1 307 7.49e-106 315
MS.gene028204.t1 AT3G50700 56.757 185 74 3 2 182 5 187 8.52e-72 233
MS.gene028204.t1 AT5G66730 57.219 187 68 4 2 182 5 185 7.60e-71 231
MS.gene028204.t1 AT4G02670 49.569 232 95 4 27 243 53 277 3.33e-70 227
MS.gene028204.t1 AT4G02670 49.569 232 95 4 27 243 55 279 3.34e-70 227
MS.gene028204.t1 AT3G45260 55.747 174 76 1 27 200 39 211 1.19e-69 227
MS.gene028204.t1 AT3G45260 55.747 174 76 1 27 200 39 211 1.19e-69 227
MS.gene028204.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.19e-69 227
MS.gene028204.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.19e-69 227
MS.gene028204.t1 AT1G55110 61.538 156 59 1 27 182 63 217 1.19e-69 227
MS.gene028204.t1 AT3G13810 62.821 156 57 1 27 182 51 205 3.02e-69 227
MS.gene028204.t1 AT3G13810 62.821 156 57 1 27 182 70 224 3.64e-69 228
MS.gene028204.t1 AT3G13810 62.821 156 57 1 27 182 70 224 3.64e-69 228
MS.gene028204.t1 AT5G60470 54.098 183 83 1 27 209 44 225 3.82e-69 226
MS.gene028204.t1 AT3G13810 62.821 156 57 1 27 182 65 219 3.91e-69 227
MS.gene028204.t1 AT5G60470 54.098 183 83 1 27 209 40 221 4.55e-69 226
MS.gene028204.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.92e-69 227
MS.gene028204.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.92e-69 227
MS.gene028204.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.92e-69 227
MS.gene028204.t1 AT2G02080 55.307 179 75 3 27 202 54 230 4.92e-69 227
MS.gene028204.t1 AT5G03150 61.538 156 59 1 27 182 53 207 5.97e-69 227
MS.gene028204.t1 AT1G03840 63.462 156 55 2 27 182 41 194 3.03e-68 225
MS.gene028204.t1 AT1G14580 58.974 156 62 2 27 182 53 206 3.22e-68 224
MS.gene028204.t1 AT1G14580 58.974 156 62 2 27 182 53 206 3.22e-68 224
MS.gene028204.t1 AT1G14580 58.974 156 62 2 27 182 67 220 4.78e-68 224
MS.gene028204.t1 AT2G02070 61.538 156 58 2 27 182 52 205 1.16e-67 226
MS.gene028204.t1 AT2G02070 61.538 156 58 2 27 182 52 205 1.16e-67 226
MS.gene028204.t1 AT5G44160 49.302 215 97 5 27 236 37 244 2.73e-66 219
MS.gene028204.t1 AT3G13810 64.138 145 51 1 38 182 68 211 7.60e-65 216
MS.gene028204.t1 AT1G03840 62.179 156 55 3 27 182 41 192 9.65e-65 216
MS.gene028204.t1 AT3G13810 64.138 145 51 1 38 182 82 225 9.68e-65 216
MS.gene028204.t1 AT5G60470 54.070 172 78 1 38 209 46 216 1.19e-64 214
MS.gene028204.t1 AT5G44160 49.268 205 92 5 37 236 8 205 6.08e-63 209
MS.gene028204.t1 AT2G02080 53.205 156 68 3 51 203 1 154 2.10e-56 192
MS.gene028204.t1 AT2G02080 53.205 156 68 3 51 203 1 154 2.10e-56 192
MS.gene028204.t1 AT5G22890 31.138 167 101 5 25 178 186 351 6.89e-17 82.0
MS.gene028204.t1 AT1G34370 29.231 195 119 7 36 216 14 203 1.09e-16 80.5
MS.gene028204.t1 AT1G34370 29.231 195 119 7 36 216 224 413 1.55e-16 82.0
MS.gene028204.t1 AT1G34370 29.231 195 119 7 36 216 224 413 1.55e-16 82.0
MS.gene028204.t1 AT3G48550 31.034 174 87 6 226 393 11 157 2.75e-15 73.6
MS.gene028204.t1 AT3G57670 30.986 142 87 5 48 181 209 347 4.34e-12 67.8

Find 75 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATGCATTTGAAGATTC+TGG 0.162069 4.2:-16266037 None:intergenic
CAGTTTCAATCTTCAACTTC+TGG 0.249687 4.2:+16268306 MS.gene028204:CDS
AATGGTTTATCAGGTGGTTT+TGG 0.294809 4.2:-16267823 None:intergenic
TATATCTGAATTAGTTAATA+AGG 0.305699 4.2:-16267857 None:intergenic
TTGAGTTTGAAAATGCTAAA+AGG 0.314430 4.2:+16268172 MS.gene028204:CDS
AGAAGAAACATCCATTGTTA+TGG 0.316252 4.2:+16268338 MS.gene028204:CDS
TCTGATTCTAACAAAGTCTT+TGG 0.339052 4.2:-16265972 None:intergenic
CTAAAATTTGCCTCACTAGA+TGG 0.342793 4.2:-16267766 None:intergenic
GCACTGGTAATCCTTGTAAT+GGG 0.366655 4.2:-16267798 None:intergenic
GGTGCTGATAATGGTTTATC+AGG 0.373734 4.2:-16267832 None:intergenic
GAAGATAAGTAACACTGTTA+TGG 0.384215 4.2:+16268257 MS.gene028204:CDS
TGATGCAAACAACTTGGTTC+TGG 0.390697 4.2:-16266134 None:intergenic
GAAGAAACATCCATTGTTAT+GGG 0.391163 4.2:+16268339 MS.gene028204:CDS
ATTGTTGTTGCTTGGAACTC+TGG 0.397896 4.2:-16267703 None:intergenic
AATAAGGTAGGTGCTGATAA+TGG 0.403602 4.2:-16267841 None:intergenic
ACACACTTGAAGCTTTCAAT+AGG 0.404368 4.2:+16267991 MS.gene028204:CDS
GAAACTGTTGCTTACAAGCT+TGG 0.418931 4.2:-16268290 None:intergenic
CCGGCTGCATTGTTGTTGCT+TGG 0.420074 4.2:-16267711 None:intergenic
TCTGGTCTCTTTGAAACCCT+TGG 0.440797 4.2:-16266019 None:intergenic
ATGTTACCTGGTGTGCCTGC+TGG 0.441395 4.2:-16265849 None:intergenic
AGAGACACAACTTCAGCATC+TGG 0.455957 4.2:-16265945 None:intergenic
AGAGTTGAAGTTCTAAGTTA+TGG 0.456212 4.2:-16267909 None:intergenic
AAAGTTGGCAATGGCAGAAA+AGG 0.462255 4.2:+16268095 MS.gene028204:CDS
AGCACTGGTAATCCTTGTAA+TGG 0.468962 4.2:-16267799 None:intergenic
ACAATGGATGTTTCTTCTGA+TGG 0.469771 4.2:-16268333 None:intergenic
TTCCCCTTCTGTGGTGGCTG+AGG 0.471434 4.2:-16268370 None:intergenic
AGTATATCAGCCCCATTACA+AGG 0.490202 4.2:+16267787 MS.gene028204:CDS
TTCTGCTTCCCCTTCTGTGG+TGG 0.491783 4.2:-16268376 None:intergenic
TTGTTGCTTGGAACTCTGGT+TGG 0.493027 4.2:-16267699 None:intergenic
ACATAGTAATCATAAACAAT+GGG 0.505280 4.2:+16266214 MS.gene028204:CDS
GTTTGTGAGATCTGTAACCA+AGG 0.507755 4.2:+16266002 MS.gene028204:CDS
TACTAGTGAGGAGCTAAAGT+TGG 0.509569 4.2:+16268080 MS.gene028204:CDS
AGAAGACCAGCAGGCACACC+AGG 0.525697 4.2:+16265843 MS.gene028204:CDS
AAACAAGCACAAGATGAACT+TGG 0.527243 4.2:+16268201 MS.gene028204:CDS
ACTTGAGGGTTTATAGAGGA+AGG 0.528633 4.2:-16267934 None:intergenic
TCATTCTGCTTCCCCTTCTG+TGG 0.531823 4.2:-16268379 None:intergenic
AACATAGTAATCATAAACAA+TGG 0.531879 4.2:+16266213 MS.gene028204:CDS
TTTGAAAGAGTTTACTAGTG+AGG 0.537749 4.2:+16268068 MS.gene028204:CDS
CATGATCCATGTCATGCACT+AGG 0.544901 4.2:+16266155 MS.gene028204:CDS
TTCTACTTGAGGGTTTATAG+AGG 0.552798 4.2:-16267938 None:intergenic
TTTGTGAGATCTGTAACCAA+GGG 0.553552 4.2:+16266003 MS.gene028204:CDS
CCAAGCAACAACAATGCAGC+CGG 0.555619 4.2:+16267711 MS.gene028204:CDS
GCTGATAATGGTTTATCAGG+TGG 0.558635 4.2:-16267829 None:intergenic
GTTGAAGTTCTAAGTTATGG+TGG 0.560774 4.2:-16267906 None:intergenic
ATGTCCTCAGCCACCACAGA+AGG 0.565952 4.2:+16268366 MS.gene028204:CDS
GGATCATGATGCAAACAACT+TGG 0.569323 4.2:-16266140 None:intergenic
AGAGGACATTCGTGTGATTG+TGG 0.573870 4.2:+16266308 MS.gene028204:CDS
ATTAACTGTGTTGTCTAACA+TGG 0.574745 4.2:-16265754 None:intergenic
CATGCACTAGGTGACCTTGT+TGG 0.575089 4.2:+16266167 MS.gene028204:CDS
GTTATGGTGGTGATGAGAAG+TGG 0.575098 4.2:-16267893 None:intergenic
ACAATCACACGAATGTCCTC+TGG 0.575624 4.2:-16266306 None:intergenic
TGATAATGCAAAAGCAGTAG+TGG 0.577782 4.2:-16265790 None:intergenic
TCTGAATTAGTTAATAAGGT+AGG 0.583687 4.2:-16267853 None:intergenic
TGTCCTCAGCCACCACAGAA+GGG 0.586753 4.2:+16268367 MS.gene028204:CDS
GTTTGTGACAAATGCAACAA+AGG 0.587309 4.2:+16266236 MS.gene028204:CDS
GAAGAAGACACAAAGTGCCA+TGG 0.587753 4.2:+16266057 MS.gene028204:CDS
AGCAGTAGTGGCATCAGAAG+AGG 0.593577 4.2:-16265778 None:intergenic
ATAATGAGTCAGAGAAACAA+AGG 0.608502 4.2:+16268016 MS.gene028204:CDS
CAAACTTGCTAGTTCCAATG+TGG 0.610019 4.2:-16268049 None:intergenic
AAGGAATGATGTATCCACAT+TGG 0.613625 4.2:+16268035 MS.gene028204:CDS
GTCCTCAGCCACCACAGAAG+GGG 0.615788 4.2:+16268368 MS.gene028204:CDS
TGAGGAGCTAAAGTTGGCAA+TGG 0.615902 4.2:+16268086 MS.gene028204:CDS
CAAGACGCATGCACAATCAG+AGG 0.629083 4.2:+16267670 MS.gene028204:CDS
ATCAAAACATGTGGTACCAG+AGG 0.630022 4.2:+16266290 MS.gene028204:CDS
CACTGGTAATCCTTGTAATG+GGG 0.639858 4.2:-16267797 None:intergenic
TGGTGGTGATGAGAAGTGGA+AGG 0.646826 4.2:-16267889 None:intergenic
AAAGCACATATCAAAACATG+TGG 0.657146 4.2:+16266281 MS.gene028204:CDS
AAGCACAAGTCCATCTAGTG+AGG 0.660327 4.2:+16267756 MS.gene028204:CDS
AATGTTTCTTGATTCCAACA+AGG 0.660532 4.2:-16266181 None:intergenic
GCAGTTCTAGAAGAACATGC+CGG 0.667398 4.2:-16267730 None:intergenic
AGGTCACCTAGTGCATGACA+TGG 0.667882 4.2:-16266161 None:intergenic
GGACATGTAACCCATAACAA+TGG 0.669538 4.2:-16268349 None:intergenic
TTAACTGTGTTGTCTAACAT+GGG 0.670601 4.2:-16265753 None:intergenic
TTTGCATTATCAGAAAATGG+GGG 0.689769 4.2:+16265801 MS.gene028204:CDS
TTGAAAAGAGAAACAACACA+AGG 0.699162 4.2:+16266086 MS.gene028204:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAATACTAATATTAATT+TGG - chr4.2:16266562-16266581 None:intergenic 10.0%
!!! ATAATAAAATAGTACTATTT+TGG + chr4.2:16266756-16266775 MS.gene028204:intron 10.0%
!! AAATTAATTAATCAAAGATC+TGG - chr4.2:16267131-16267150 None:intergenic 15.0%
!! AATTAATTAATCAAAGATCT+GGG - chr4.2:16267130-16267149 None:intergenic 15.0%
!! ATTGATAAAAAAATGTAGAT+AGG - chr4.2:16266980-16266999 None:intergenic 15.0%
!! TAGCTAATATTATAAGTTTA+TGG + chr4.2:16266942-16266961 MS.gene028204:intron 15.0%
!! TATATCTGAATTAGTTAATA+AGG - chr4.2:16267860-16267879 None:intergenic 15.0%
!! TTTCATCTTAAAAAATAACA+CGG - chr4.2:16267178-16267197 None:intergenic 15.0%
!!! ACTCTCAAATATAAAATTTT+AGG - chr4.2:16266652-16266671 None:intergenic 15.0%
!!! CTCTCAAATATAAAATTTTA+GGG - chr4.2:16266651-16266670 None:intergenic 15.0%
!!! TTTTTATAACCTTTTTTTGT+GGG + chr4.2:16265911-16265930 MS.gene028204:intron 15.0%
!!! TTTTTTATAACCTTTTTTTG+TGG + chr4.2:16265910-16265929 MS.gene028204:intron 15.0%
!! AACATAGTAATCATAAACAA+TGG + chr4.2:16266213-16266232 MS.gene028204:CDS 20.0%
!! AATTAAATTCTCATCTTCTT+AGG + chr4.2:16266711-16266730 MS.gene028204:intron 20.0%
!! ACATAGTAATCATAAACAAT+GGG + chr4.2:16266214-16266233 MS.gene028204:CDS 20.0%
!! AGTCAATAACCATATATTAT+AGG - chr4.2:16267602-16267621 None:intergenic 20.0%
!!! GTCAAATTATATTTTCATAC+AGG + chr4.2:16267413-16267432 MS.gene028204:intron 20.0%
!!! TTTTATCAATAAACCAAACA+AGG + chr4.2:16266990-16267009 MS.gene028204:intron 20.0%
! AGGTACTAAAATGAATCATA+TGG + chr4.2:16267010-16267029 MS.gene028204:intron 25.0%
! ATAAGACAGAATGATGAAAT+AGG - chr4.2:16267069-16267088 None:intergenic 25.0%
! ATATATATATAGGCCTTGAT+TGG + chr4.2:16267258-16267277 MS.gene028204:intron 25.0%
! ATGAGAATTTAATTGCAAAG+AGG - chr4.2:16266705-16266724 None:intergenic 25.0%
! GAAACTATCTACAATAAAGA+TGG - chr4.2:16266411-16266430 None:intergenic 25.0%
! TAAGACAGAATGATGAAATA+GGG - chr4.2:16267068-16267087 None:intergenic 25.0%
! TAAGATGAAATAGCTATAAC+AGG + chr4.2:16267188-16267207 MS.gene028204:intron 25.0%
! TACAATAAAGATGGAAAACT+TGG - chr4.2:16266402-16266421 None:intergenic 25.0%
! TACTAAAATGAATCATATGG+AGG + chr4.2:16267013-16267032 MS.gene028204:intron 25.0%
! TATTTGTAAGATATGTTACC+TGG - chr4.2:16265864-16265883 None:intergenic 25.0%
! TCTGAATTAGTTAATAAGGT+AGG - chr4.2:16267856-16267875 None:intergenic 25.0%
! TGAGAATTTAATTGCAAAGA+GGG - chr4.2:16266704-16266723 None:intergenic 25.0%
! TTGAGTTTGAAAATGCTAAA+AGG + chr4.2:16268172-16268191 MS.gene028204:CDS 25.0%
!! CTTTTGCATTATCAGAAAAT+GGG + chr4.2:16265799-16265818 MS.gene028204:CDS 25.0%
!! TTTTCTCTCTCTTTCTTTTT+CGG + chr4.2:16266439-16266458 MS.gene028204:intron 25.0%
!! TTTTGCATTATCAGAAAATG+GGG + chr4.2:16265800-16265819 MS.gene028204:CDS 25.0%
!!! AATTTGGTTTTTCTACTTGA+GGG - chr4.2:16267951-16267970 None:intergenic 25.0%
!!! ATTCATTTTAGTACCTTGTT+TGG - chr4.2:16267006-16267025 None:intergenic 25.0%
!!! TAGTTTTGATCGTTTGAATT+TGG - chr4.2:16267967-16267986 None:intergenic 25.0%
AAAGCACATATCAAAACATG+TGG + chr4.2:16266281-16266300 MS.gene028204:CDS 30.0%
AATGTTTCTTGATTCCAACA+AGG - chr4.2:16266184-16266203 None:intergenic 30.0%
ACAAACTGTTTATCCAATCA+AGG - chr4.2:16267274-16267293 None:intergenic 30.0%
AGAAGAAACATCCATTGTTA+TGG + chr4.2:16268338-16268357 MS.gene028204:CDS 30.0%
ATAATGAGTCAGAGAAACAA+AGG + chr4.2:16268016-16268035 MS.gene028204:CDS 30.0%
CAATATGAGAAAGTGAAAGA+TGG - chr4.2:16266370-16266389 None:intergenic 30.0%
CCAAACAGACCTATAATATA+TGG + chr4.2:16267590-16267609 MS.gene028204:intron 30.0%
CCATATATTATAGGTCTGTT+TGG - chr4.2:16267593-16267612 None:intergenic 30.0%
GAAGAAACATCCATTGTTAT+GGG + chr4.2:16268339-16268358 MS.gene028204:CDS 30.0%
GAAGATAAGTAACACTGTTA+TGG + chr4.2:16268257-16268276 MS.gene028204:CDS 30.0%
GTTCATCATTCATCAAATTC+AGG + chr4.2:16266498-16266517 MS.gene028204:intron 30.0%
TAGTAGCTACTTAATGATAC+TGG - chr4.2:16266920-16266939 None:intergenic 30.0%
TCTGATTCTAACAAAGTCTT+TGG - chr4.2:16265975-16265994 None:intergenic 30.0%
TTACATTTGCCCTTTGATTA+GGG + chr4.2:16267627-16267646 MS.gene028204:intron 30.0%
TTCTATGCATTTGAAGATTC+TGG - chr4.2:16266040-16266059 None:intergenic 30.0%
TTGAAAAGAGAAACAACACA+AGG + chr4.2:16266086-16266105 MS.gene028204:CDS 30.0%
TTTGAAAGAGTTTACTAGTG+AGG + chr4.2:16268068-16268087 MS.gene028204:CDS 30.0%
TTTGCATTATCAGAAAATGG+GGG + chr4.2:16265801-16265820 MS.gene028204:CDS 30.0%
! CAATTGACTGTGTTTGTTAA+CGG + chr4.2:16266533-16266552 MS.gene028204:intron 30.0%
! GCTTTTGCATTATCAGAAAA+TGG + chr4.2:16265798-16265817 MS.gene028204:CDS 30.0%
! TCTCTTTTCAACAATTTCCA+TGG - chr4.2:16266077-16266096 None:intergenic 30.0%
! TTTTTACATGCTCTCTCAAA+CGG + chr4.2:16267523-16267542 MS.gene028204:intron 30.0%
!! AGAGTTGAAGTTCTAAGTTA+TGG - chr4.2:16267912-16267931 None:intergenic 30.0%
!! GAAGAGTTAATTTGTACTAG+TGG + chr4.2:16267039-16267058 MS.gene028204:intron 30.0%
!!! GAATTTGGTTTTTCTACTTG+AGG - chr4.2:16267952-16267971 None:intergenic 30.0%
AAAAGAAAAAGAAGACCAGC+AGG + chr4.2:16265834-16265853 MS.gene028204:CDS 35.0%
AAACAAGCACAAGATGAACT+TGG + chr4.2:16268201-16268220 MS.gene028204:CDS 35.0%
AAGGAATGATGTATCCACAT+TGG + chr4.2:16268035-16268054 MS.gene028204:CDS 35.0%
ACAATGGATGTTTCTTCTGA+TGG - chr4.2:16268336-16268355 None:intergenic 35.0%
ACACACTTGAAGCTTTCAAT+AGG + chr4.2:16267991-16268010 MS.gene028204:CDS 35.0%
CAGTTTCAATCTTCAACTTC+TGG + chr4.2:16268306-16268325 MS.gene028204:CDS 35.0%
CTAAAATTTGCCTCACTAGA+TGG - chr4.2:16267769-16267788 None:intergenic 35.0%
CTAAACACACCCACAAAAAA+AGG - chr4.2:16265923-16265942 None:intergenic 35.0%
GTTACATTTGCCCTTTGATT+AGG + chr4.2:16267626-16267645 MS.gene028204:intron 35.0%
GTTTGTGACAAATGCAACAA+AGG + chr4.2:16266236-16266255 MS.gene028204:CDS 35.0%
TTAATGATACTGGCACTTAC+AGG - chr4.2:16266910-16266929 None:intergenic 35.0%
TTCTACTTGAGGGTTTATAG+AGG - chr4.2:16267941-16267960 None:intergenic 35.0%
TTTGTGAGATCTGTAACCAA+GGG + chr4.2:16266003-16266022 MS.gene028204:CDS 35.0%
! AATGGTTTATCAGGTGGTTT+TGG - chr4.2:16267826-16267845 None:intergenic 35.0%
! TGATAATGCAAAAGCAGTAG+TGG - chr4.2:16265793-16265812 None:intergenic 35.0%
!! AATAAGGTAGGTGCTGATAA+TGG - chr4.2:16267844-16267863 None:intergenic 35.0%
!! GTTGAAGTTCTAAGTTATGG+TGG - chr4.2:16267909-16267928 None:intergenic 35.0%
!!! ATTTTAGGGTTTGACTTCCT+AGG - chr4.2:16266637-16266656 None:intergenic 35.0%
AAACTCTCCACCCTAATCAA+AGG - chr4.2:16267640-16267659 None:intergenic 40.0%
AAAGCCTGCATAATGATGAG+GGG - chr4.2:16266879-16266898 None:intergenic 40.0%
AAAGTTGGCAATGGCAGAAA+AGG + chr4.2:16268095-16268114 MS.gene028204:CDS 40.0%
AACTCTCCACCCTAATCAAA+GGG - chr4.2:16267639-16267658 None:intergenic 40.0%
AAGAAAGCAGAACACAAACC+TGG - chr4.2:16266345-16266364 None:intergenic 40.0%
ACAGTTCTTAGGACTGTAGT+AGG + chr4.2:16267550-16267569 MS.gene028204:intron 40.0%
ACTTGAGGGTTTATAGAGGA+AGG - chr4.2:16267937-16267956 None:intergenic 40.0%
AGTATATCAGCCCCATTACA+AGG + chr4.2:16267787-16267806 MS.gene028204:CDS 40.0%
ATCAAAACATGTGGTACCAG+AGG + chr4.2:16266290-16266309 MS.gene028204:CDS 40.0%
CAAACGGTTCAACAGTTCTT+AGG + chr4.2:16267539-16267558 MS.gene028204:intron 40.0%
CAAAGCCTGCATAATGATGA+GGG - chr4.2:16266880-16266899 None:intergenic 40.0%
CACTGGTAATCCTTGTAATG+GGG - chr4.2:16267800-16267819 None:intergenic 40.0%
GGACATGTAACCCATAACAA+TGG - chr4.2:16268352-16268371 None:intergenic 40.0%
GGATCATGATGCAAACAACT+TGG - chr4.2:16266143-16266162 None:intergenic 40.0%
GTTTGTGAGATCTGTAACCA+AGG + chr4.2:16266002-16266021 MS.gene028204:CDS 40.0%
TACTAGTGAGGAGCTAAAGT+TGG + chr4.2:16268080-16268099 MS.gene028204:CDS 40.0%
TGATGCAAACAACTTGGTTC+TGG - chr4.2:16266137-16266156 None:intergenic 40.0%
! ATTGTTGTTGCTTGGAACTC+TGG - chr4.2:16267706-16267725 None:intergenic 40.0%
! GAAACTGTTGCTTACAAGCT+TGG - chr4.2:16268293-16268312 None:intergenic 40.0%
! GCACTGGTAATCCTTGTAAT+GGG - chr4.2:16267801-16267820 None:intergenic 40.0%
!! AGCACTGGTAATCCTTGTAA+TGG - chr4.2:16267802-16267821 None:intergenic 40.0%
!! CAAACTTGCTAGTTCCAATG+TGG - chr4.2:16268052-16268071 None:intergenic 40.0%
!! GCTGATAATGGTTTATCAGG+TGG - chr4.2:16267832-16267851 None:intergenic 40.0%
!! GGTGCTGATAATGGTTTATC+AGG - chr4.2:16267835-16267854 None:intergenic 40.0%
AAGCACAAGTCCATCTAGTG+AGG + chr4.2:16267756-16267775 MS.gene028204:CDS 45.0%
AAGCCTGCATAATGATGAGG+GGG - chr4.2:16266878-16266897 None:intergenic 45.0%
ACAATCACACGAATGTCCTC+TGG - chr4.2:16266309-16266328 None:intergenic 45.0%
AGAGACACAACTTCAGCATC+TGG - chr4.2:16265948-16265967 None:intergenic 45.0%
AGAGGACATTCGTGTGATTG+TGG + chr4.2:16266308-16266327 MS.gene028204:CDS 45.0%
AGATCTGGGTCATGACTCAT+AGG - chr4.2:16267116-16267135 None:intergenic 45.0%
CATGATCCATGTCATGCACT+AGG + chr4.2:16266155-16266174 MS.gene028204:CDS 45.0%
CATTTGCCCTTTGATTAGGG+TGG + chr4.2:16267630-16267649 MS.gene028204:intron 45.0%
GCAAAGCCTGCATAATGATG+AGG - chr4.2:16266881-16266900 None:intergenic 45.0%
TGAGGAGCTAAAGTTGGCAA+TGG + chr4.2:16268086-16268105 MS.gene028204:CDS 45.0%
! ATTGTGGTCGTGTGTTTTCC+AGG + chr4.2:16266324-16266343 MS.gene028204:CDS 45.0%
! GAAGAAGACACAAAGTGCCA+TGG + chr4.2:16266057-16266076 MS.gene028204:CDS 45.0%
! GTTATGGTGGTGATGAGAAG+TGG - chr4.2:16267896-16267915 None:intergenic 45.0%
! GTTTGACTTCCTAGGAAGCT+AGG - chr4.2:16266629-16266648 None:intergenic 45.0%
! TTGTTGCTTGGAACTCTGGT+TGG - chr4.2:16267702-16267721 None:intergenic 45.0%
!! GCAGTTCTAGAAGAACATGC+CGG - chr4.2:16267733-16267752 None:intergenic 45.0%
!! TCTGGTCTCTTTGAAACCCT+TGG - chr4.2:16266022-16266041 None:intergenic 45.0%
!! ATAGTTTAATATATATATAT+AGG + chr4.2:16267248-16267267 MS.gene028204:intron 5.0%
AGCAGTAGTGGCATCAGAAG+AGG - chr4.2:16265781-16265800 None:intergenic 50.0%
AGGTCACCTAGTGCATGACA+TGG - chr4.2:16266164-16266183 None:intergenic 50.0%
ATAATGATGAGGGGGTGGGT+GGG - chr4.2:16266870-16266889 None:intergenic 50.0%
CAAGACGCATGCACAATCAG+AGG + chr4.2:16267670-16267689 MS.gene028204:CDS 50.0%
CATGCACTAGGTGACCTTGT+TGG + chr4.2:16266167-16266186 MS.gene028204:CDS 50.0%
CCAAGCAACAACAATGCAGC+CGG + chr4.2:16267711-16267730 MS.gene028204:CDS 50.0%
CTGCATAATGATGAGGGGGT+GGG - chr4.2:16266874-16266893 None:intergenic 50.0%
TCATTCTGCTTCCCCTTCTG+TGG - chr4.2:16268382-16268401 None:intergenic 50.0%
TCTTCTGCACCTAGCTTCCT+AGG + chr4.2:16266617-16266636 MS.gene028204:intron 50.0%
TGTGTGTGAGTGAGTGTGTG+TGG + chr4.2:16266476-16266495 MS.gene028204:intron 50.0%
! TGGTGGTGATGAGAAGTGGA+AGG - chr4.2:16267892-16267911 None:intergenic 50.0%
!!! TCAGGTGGTTTTGGAAGCAC+TGG - chr4.2:16267817-16267836 None:intergenic 50.0%
ATGTCCTCAGCCACCACAGA+AGG + chr4.2:16268366-16268385 MS.gene028204:CDS 55.0%
ATGTTACCTGGTGTGCCTGC+TGG - chr4.2:16265852-16265871 None:intergenic 55.0%
CATAATGATGAGGGGGTGGG+TGG - chr4.2:16266871-16266890 None:intergenic 55.0%
CCACCCCCTCATCATTATGC+AGG + chr4.2:16266872-16266891 MS.gene028204:intron 55.0%
CCTGCATAATGATGAGGGGG+TGG - chr4.2:16266875-16266894 None:intergenic 55.0%
TGTCCTCAGCCACCACAGAA+GGG + chr4.2:16268367-16268386 MS.gene028204:CDS 55.0%
TTCTGCTTCCCCTTCTGTGG+TGG - chr4.2:16268379-16268398 None:intergenic 55.0%
! CCGGCTGCATTGTTGTTGCT+TGG - chr4.2:16267714-16267733 None:intergenic 55.0%
AGAAGACCAGCAGGCACACC+AGG + chr4.2:16265843-16265862 MS.gene028204:CDS 60.0%
GTCCTCAGCCACCACAGAAG+GGG + chr4.2:16268368-16268387 MS.gene028204:CDS 60.0%
TTCCCCTTCTGTGGTGGCTG+AGG - chr4.2:16268373-16268392 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 16265756 16268401 16265756 ID=MS.gene028204
chr4.2 mRNA 16265756 16268401 16265756 ID=MS.gene028204.t1;Parent=MS.gene028204
chr4.2 exon 16265756 16265864 16265756 ID=MS.gene028204.t1.exon1;Parent=MS.gene028204.t1
chr4.2 CDS 16265756 16265864 16265756 ID=cds.MS.gene028204.t1;Parent=MS.gene028204.t1
chr4.2 exon 16265943 16266345 16265943 ID=MS.gene028204.t1.exon2;Parent=MS.gene028204.t1
chr4.2 CDS 16265943 16266345 16265943 ID=cds.MS.gene028204.t1;Parent=MS.gene028204.t1
chr4.2 exon 16267648 16268401 16267648 ID=MS.gene028204.t1.exon3;Parent=MS.gene028204.t1
chr4.2 CDS 16267648 16268401 16267648 ID=cds.MS.gene028204.t1;Parent=MS.gene028204.t1
Gene Sequence

>MS.gene028204

ATGTTAGACAACACAGTTAATTCCTCTTCTGATGCCACTACTGCTTTTGCATTATCAGAAAATGGGGGTGCTAATAACAAAAGAAAAAGAAGACCAGCAGGCACACCAGGTAACATATCTTACAAATAAAGATTACTTCTTTTTTAACTAAGTATTTTTTATAACCTTTTTTTGTGGGTGTGTTTAGATCCAGATGCTGAAGTTGTGTCTCTATCACCAAAGACTTTGTTAGAATCAGACAGATATGTTTGTGAGATCTGTAACCAAGGGTTTCAAAGAGACCAGAATCTTCAAATGCATAGAAGAAGACACAAAGTGCCATGGAAATTGTTGAAAAGAGAAACAACACAAGGATTAAAAAAGAGAGTATTTGTTTGTCCAGAACCAAGTTGTTTGCATCATGATCCATGTCATGCACTAGGTGACCTTGTTGGAATCAAGAAACATTTTAGAAGAAAACATAGTAATCATAAACAATGGGTTTGTGACAAATGCAACAAAGGTTATGCTGTTCAATCTGATTATAAAGCACATATCAAAACATGTGGTACCAGAGGACATTCGTGTGATTGTGGTCGTGTGTTTTCCAGGTTTGTGTTCTGCTTTCTTTTCCATCTTTCACTTTCTCATATTGTTCTTCATTCCAAGTTTTCCATCTTTATTGTAGATAGTTTCTTTTCTCTTTTTCTCTCTCTTTCTTTTTCGGTGTGTGTGTGTGTGTGTGTGTGAGTGAGTGTGTGTGGTTCATCATTCATCAAATTCAGGTCACTGCTGAAACAATTGACTGTGTTTGTTAACGGAAACCAAATTAATATTAGTATTTTACTACTTCAATTATACTATATTATATTATTTGATTATTCTTCTGCACCTAGCTTCCTAGGAAGTCAAACCCTAAAATTTTATATTTGAGAGTGAAACAAAAATTCTTTTAAATCTCAGATACCCTCTTTGCAATTAAATTCTCATCTTCTTAGGTTATCATATTATTATTCTTCATATAATAAAATAGTACTATTTTGGAAAGTTAAATACATAAGCTGAAAATTTTAGCATTATGTTTTGAAATTTTGAATATATGAATATGATATGATATGAATAGTGAATACAGCCCACCCACCCCCTCATCATTATGCAGGCTTTGCAAATTGCCTGTAAGTGCCAGTATCATTAAGTAGCTACTAGCTAGCTAATATTATAAGTTTATGGACTGTATTATTACCTATCTACATTTTTTTATCAATAAACCAAACAAGGTACTAAAATGAATCATATGGAGGAGAGAAGAGTTAATTTGTACTAGTGGAGACCCTATTTCATCATTCTGTCTTATTTAATTAATTAATTAATTACTTTTCCTATGAGTCATGACCCAGATCTTTGATTAATTAATTTTTTTATAAACATATCTTTGTTTGACCGTGTTATTTTTTAAGATGAAATAGCTATAACAGGCATTAACATATATAATATCACAATCTAGTCATATAATATAGTTTAATATATATATATAGGCCTTGATTGGATAAACAGTTTGTTTAATTATGTGATTATATAACATAGATGCTTATTATTCATGTGTTTATATAGAAACTTTTTTTATAAGATGAAATAAAGTCAAATTATGTATAAACTTTTTTTTATAAGATAAAATAAAGTCAAATTATATTTTCATACAGGATATACTTAATCTCTTTTCAAAACTATCATCATGATGAACTTCATGAACACACTTAAAACAGTTTATAGACATGTTATTATAAATTATTTTTACATGCTCTCTCAAACGGTTCAACAGTTCTTAGGACTGTAGTAGGTAAACTCATAAATAAATCCAAACAGACCTATAATATATGGTTATTGACTTAAAGTTACATTTGCCCTTTGATTAGGGTGGAGAGTTTCATAGAACATCAAGACGCATGCACAATCAGAGGAAAGCACCAACCAGAGTTCCAAGCAACAACAATGCAGCCGGCATGTTCTTCTAGAACTGCTTCAAGCACAAGTCCATCTAGTGAGGCAAATTTTAGTATATCAGCCCCATTACAAGGATTACCAGTGCTTCCAAAACCACCTGATAAACCATTATCAGCACCTACCTTATTAACTAATTCAGATATACATAACAAGCCTTCCACTTCTCATCACCACCATAACTTAGAACTTCAACTCTTACCTTCCTCTATAAACCCTCAAGTAGAAAAACCAAATTCAAACGATCAAAACTACGAAACACACTTGAAGCTTTCAATAGGAAATAATGAGTCAGAGAAACAAAGGAATGATGTATCCACATTGGAACTAGCAAGTTTGAAAGAGTTTACTAGTGAGGAGCTAAAGTTGGCAATGGCAGAAAAGGCTTATGCAGAAGAAGCTAGAAAAGAAGCTAAACGTCAAATTGAGATAGCTGAAGTTGAGTTTGAAAATGCTAAAAGGATAAGAAAACAAGCACAAGATGAACTTGGAAAAGCTGAAGCTTTGAGAAAACAAGCTATTAAGAAGATAAGTAACACTGTTATGGAAGTAACATGCCAAGCTTGTAAGCAACAGTTTCAATCTTCAACTTCTGGTGTTCCATCAGAAGAAACATCCATTGTTATGGGTTACATGTCCTCAGCCACCACAGAAGGGGAAGCAGAATGA
Protein sequence

>MS.gene028204.t1

MLDNTVNSSSDATTAFALSENGGANNKRKRRPAGTPDPDAEVVSLSPKTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRETTQGLKKRVFVCPEPSCLHHDPCHALGDLVGIKKHFRRKHSNHKQWVCDKCNKGYAVQSDYKAHIKTCGTRGHSCDCGRVFSRVESFIEHQDACTIRGKHQPEFQATTMQPACSSRTASSTSPSSEANFSISAPLQGLPVLPKPPDKPLSAPTLLTNSDIHNKPSTSHHHHNLELQLLPSSINPQVEKPNSNDQNYETHLKLSIGNNESEKQRNDVSTLELASLKEFTSEELKLAMAEKAYAEEARKEAKRQIEIAEVEFENAKRIRKQAQDELGKAEALRKQAIKKISNTVMEVTCQACKQQFQSSTSGVPSEETSIVMGYMSSATTEGEAE