AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029813

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029813.t1 MTR_1g064060 100.000 341 0 0 1 341 1 341 0.0 710
MS.gene029813.t1 MTR_4g461070 96.188 341 13 0 1 341 1 341 0.0 686
MS.gene029813.t1 MTR_1g064060 98.997 299 3 0 1 299 1 299 0.0 615
MS.gene029813.t1 MTR_3g112040 25.382 327 212 14 12 326 27 333 2.85e-12 67.4
MS.gene029813.t1 MTR_3g081400 63.636 44 13 1 263 306 34 74 2.27e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029813.t1 AT5G03300 83.728 338 55 0 4 341 8 345 0.0 608
MS.gene029813.t1 AT3G09820 82.891 339 58 0 3 341 6 344 0.0 598
MS.gene029813.t1 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MS.gene029813.t1 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MS.gene029813.t1 AT3G09820 84.068 295 47 0 47 341 8 302 0.0 526
MS.gene029813.t1 AT1G19600 25.904 332 217 14 14 336 23 334 6.87e-13 69.3
MS.gene029813.t1 AT4G27600 23.962 313 209 10 7 308 123 417 1.50e-11 65.5

Find 92 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGAAGCAACCCGAAAAT+TGG 0.173309 1.2:-48896136 MS.gene029813:CDS
ACCAGCTTGTTTAGAGTTCT+TGG 0.208302 1.2:+48896549 None:intergenic
CATTCTGGGATTCAGGTATA+TGG 0.238438 1.2:-48895698 MS.gene029813:intron
GTATCAACTAATTTCTCTTT+AGG 0.253965 1.2:+48895376 None:intergenic
CCTCTGATGCCTTTGGCAAC+TGG 0.264995 1.2:+48895491 None:intergenic
TCTTTAGGTAATAGTATAAC+AGG 0.271758 1.2:+48895391 None:intergenic
GCCAAATATTTCTATATTGC+TGG 0.282337 1.2:-48896000 MS.gene029813:CDS
GCCAGCAATATAGAAATATT+TGG 0.289493 1.2:+48895999 None:intergenic
GCAACTAATTGAATGGATTC+AGG 0.289892 1.2:+48895958 None:intergenic
GATGTACTCCACATTATATT+TGG 0.305088 1.2:+48896886 None:intergenic
TAATGAAAATCAGGCTTCTC+TGG 0.314330 1.2:+48895074 None:intergenic
CAGTGGATGCTTCAAGTTCC+CGG 0.330838 1.2:-48896625 MS.gene029813:CDS
TTGAAGAACTCGCAGATAAA+TGG 0.334608 1.2:+48895809 None:intergenic
TCCATCAATAATAATGGCTT+TGG 0.352044 1.2:-48898007 None:intergenic
TGGAGGATTTCTTTCACAAT+TGG 0.357365 1.2:-48895180 MS.gene029813:CDS
CTCTGATGCCTTTGGCAACT+GGG 0.358279 1.2:+48895492 None:intergenic
TTGCGTGCCTCTGATGCCTT+TGG 0.366644 1.2:+48895484 None:intergenic
GCTCTCACACATTCTTCAAT+AGG 0.380613 1.2:+48895146 None:intergenic
ACTTGTGCTGTTTGTGTGGT+TGG 0.384223 1.2:-48896379 MS.gene029813:CDS
CAAGAATGGCATTGTTCAAC+TGG 0.389961 1.2:+48897786 None:intergenic
TTTGTAGGAGATGCCTTTGT+TGG 0.393317 1.2:-48895200 MS.gene029813:intron
CATAGATTTGTGCTTATCTT+CGG 0.395196 1.2:+48897763 None:intergenic
AGAATCACTTAATGAAAATC+AGG 0.396079 1.2:+48895065 None:intergenic
GGAAGGTGTTCTTCTCGGTA+TGG 0.399130 1.2:-48897986 MS.gene029813:CDS
CAACCCGAAAATTGGGCATT+AGG 0.401588 1.2:-48896128 MS.gene029813:intron
TTTGAAGCAACCCGAAAATT+GGG 0.412056 1.2:-48896135 MS.gene029813:CDS
ATTTATCTGCGAGTTCTTCA+AGG 0.419149 1.2:-48895807 MS.gene029813:CDS
CTGTTTCAGGTACGATGAAA+TGG 0.420585 1.2:-48896912 MS.gene029813:intron
GGTAAGTGCAGCCAGACCTT+TGG 0.421838 1.2:+48895096 None:intergenic
GCTTTGGAAGGTGTTCTTCT+CGG 0.423155 1.2:-48897991 MS.gene029813:CDS
TACCTCCCAGCCATGAACTT+TGG 0.426161 1.2:+48895636 None:intergenic
GTGCTTATCTTCGGCAAGAA+TGG 0.435280 1.2:+48897772 None:intergenic
GAATTCAATCAGGGTTGCTC+AGG 0.437505 1.2:-48896740 MS.gene029813:intron
TGCATCGGAAAAGACAAGTT+TGG 0.438686 1.2:-48896583 MS.gene029813:CDS
GTGCTACTCAGAATTCAATC+AGG 0.452939 1.2:-48896750 MS.gene029813:CDS
GTAGGAGATGCCTTTGTTGG+AGG 0.455368 1.2:-48895197 MS.gene029813:intron
ACCAATGTAACTAGTGGCCC+CGG 0.467806 1.2:+48896606 None:intergenic
AGTGGATGCTTCAAGTTCCC+GGG 0.474413 1.2:-48896624 MS.gene029813:CDS
GTTATTTGCTGCGGCATGCT+CGG 0.475346 1.2:+48895936 None:intergenic
AAGGGCAATACCTGCTCCAT+TGG 0.476251 1.2:+48895354 None:intergenic
GCAATTTGCAGCTGATAAGT+TGG 0.489440 1.2:+48896172 None:intergenic
TTTGTGTGGTTGGTGGTGAA+AGG 0.492976 1.2:-48896369 MS.gene029813:intron
CAACTAATTGAATGGATTCA+GGG 0.497187 1.2:+48895959 None:intergenic
GAAGGTGTTCTTCTCGGTAT+GGG 0.502474 1.2:-48897985 MS.gene029813:CDS
CCCGTATGTGTTGCTCAAGA+TGG 0.505635 1.2:-48895430 MS.gene029813:CDS
AATGTTATCATCCAAAGGTC+TGG 0.508644 1.2:-48895107 MS.gene029813:CDS
TGGATTCAGGGGATACAGTA+AGG 0.509166 1.2:+48895971 None:intergenic
AGGCATCAGAGGCACGCAAA+AGG 0.511205 1.2:-48895480 MS.gene029813:CDS
CCCGGGGCCACTAGTTACAT+TGG 0.515125 1.2:-48896607 MS.gene029813:CDS
CCCATCTTGAGCAACACATA+CGG 0.516893 1.2:+48895429 None:intergenic
TGTGCTGTTTGTGTGGTTGG+TGG 0.518619 1.2:-48896376 MS.gene029813:CDS
CCGTATGTGTTGCTCAAGAT+GGG 0.523351 1.2:-48895429 MS.gene029813:CDS
TACGGCAGAGCTTTCTCCTG+TGG 0.523507 1.2:+48895782 None:intergenic
TATAATGTGGAGTACATCGC+TGG 0.524618 1.2:-48896881 MS.gene029813:CDS
AATGAAAATCAGGCTTCTCT+GGG 0.526760 1.2:+48895075 None:intergenic
GAGAAATTAGTTGATACCAA+TGG 0.529177 1.2:-48895370 MS.gene029813:CDS
CGCAGATAAATGGTGCAGAA+AGG 0.536901 1.2:+48895819 None:intergenic
CGAGTTCTTCAAGGATCCAC+AGG 0.539544 1.2:-48895798 MS.gene029813:CDS
ATTGAAGAATGTGTGAGAGC+TGG 0.541729 1.2:-48895143 MS.gene029813:CDS
TTAGTTGATACCAATGGAGC+AGG 0.545602 1.2:-48895364 MS.gene029813:intron
GAAGATGAGAATACACCTAC+AGG 0.551303 1.2:-48896403 MS.gene029813:CDS
ATGCTCGGCAACTAATTGAA+TGG 0.551334 1.2:+48895951 None:intergenic
GACAGCAGAGATGTCGAGAA+GGG 0.552559 1.2:+48897957 None:intergenic
CCATCTTGAGCAACACATAC+GGG 0.552682 1.2:+48895430 None:intergenic
AGGAACTTGTGCTGTTTGTG+TGG 0.555282 1.2:-48896383 MS.gene029813:CDS
CGACAGCAGAGATGTCGAGA+AGG 0.569277 1.2:+48897956 None:intergenic
ACTAGTTACATTGGTTGCAT+CGG 0.569490 1.2:-48896598 MS.gene029813:CDS
TTGAAGAATGTGTGAGAGCT+GGG 0.569874 1.2:-48895142 MS.gene029813:CDS
ACCAAGAACTCTAAACAAGC+TGG 0.574572 1.2:-48896550 MS.gene029813:CDS
CCAGTTGCCAAAGGCATCAG+AGG 0.578685 1.2:-48895491 MS.gene029813:CDS
TCTAACCTTGTTATTTGCTG+CGG 0.578850 1.2:+48895927 None:intergenic
TGAAAGAAATCCTCCAACAA+AGG 0.578951 1.2:+48895187 None:intergenic
ATCAGCTGCAAATTGCTACA+AGG 0.584960 1.2:-48896165 MS.gene029813:CDS
AAAGATATCCCAGTTGCCAA+AGG 0.593183 1.2:-48895500 MS.gene029813:CDS
TCAATAATAATGGCTTTGGA+AGG 0.597485 1.2:-48898003 None:intergenic
CAGCAAATGTTATCATCCAA+AGG 0.601761 1.2:-48895112 MS.gene029813:CDS
AACTAATTGAATGGATTCAG+GGG 0.625445 1.2:+48895960 None:intergenic
TGCTACTCAGAATTCAATCA+GGG 0.630239 1.2:-48896749 MS.gene029813:CDS
AAGGATTACTGTTATCACAC+AGG 0.637867 1.2:-48895461 MS.gene029813:CDS
ACAGCAGAGATGTCGAGAAG+GGG 0.640848 1.2:+48897958 None:intergenic
CTTTAGGTAATAGTATAACA+GGG 0.644006 1.2:+48895392 None:intergenic
TTCCAAAGTTCATGGCTGGG+AGG 0.650106 1.2:-48895638 MS.gene029813:intron
CGGAAAAGACAAGTTTGGTG+AGG 0.651267 1.2:-48896578 MS.gene029813:CDS
GATGCAACCAATGTAACTAG+TGG 0.655384 1.2:+48896600 None:intergenic
CCAATGTAACTAGTGGCCCC+GGG 0.655549 1.2:+48896607 None:intergenic
CAAACAGCACAAGTTCCTGT+AGG 0.659898 1.2:+48896388 None:intergenic
GCATGCCGCAGCAAATAACA+AGG 0.662561 1.2:-48895932 MS.gene029813:intron
AGGATTACTGTTATCACACA+GGG 0.665946 1.2:-48895460 MS.gene029813:CDS
GTGGATGCTTCAAGTTCCCG+GGG 0.678166 1.2:-48896623 MS.gene029813:CDS
TGACCAGACTGAAAATGTCG+AGG 0.681552 1.2:-48895533 MS.gene029813:intron
AATGTGGAGTACATCGCTGG+AGG 0.683186 1.2:-48896878 MS.gene029813:intron
AATGGCTGCCAAATATAATG+TGG 0.689459 1.2:-48896894 MS.gene029813:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTTCAAAATTTTAT+CGG - chr1.2:48895795-48895814 MS.gene029813:CDS 10.0%
!! TTATTTGAATTTAACAGTTT+AGG + chr1.2:48897316-48897335 None:intergenic 15.0%
!!! AATTTTAAAACTGAATTCAA+CGG + chr1.2:48896609-48896628 None:intergenic 15.0%
!!! GTAATTTCTTTTATATTAGT+TGG - chr1.2:48896362-48896381 MS.gene029813:intron 15.0%
!!! TATTTTTATCTCCATTAAAT+GGG - chr1.2:48896986-48897005 MS.gene029813:intron 15.0%
!!! TTTATGATTTTTCTTTTTTC+AGG - chr1.2:48896295-48896314 MS.gene029813:intron 15.0%
!!! TTTTTAATAAATAGTTGAGA+AGG - chr1.2:48897045-48897064 MS.gene029813:intron 15.0%
!!! TTTTTTCTTATTGATCTTTA+AGG - chr1.2:48897341-48897360 MS.gene029813:intron 15.0%
!! ACAATTAGATGCAATAAAAA+AGG + chr1.2:48896862-48896881 None:intergenic 20.0%
!! ACTAAATTCATTTAACAACT+AGG - chr1.2:48896630-48896649 MS.gene029813:intron 20.0%
!! ATCACATCAAATCTTTAATT+AGG + chr1.2:48895992-48896011 None:intergenic 20.0%
!! ATTTGATGTGATGTAATAAT+TGG - chr1.2:48896000-48896019 MS.gene029813:CDS 20.0%
!! GAGACATAGATTAATATATA+CGG - chr1.2:48895553-48895572 MS.gene029813:intron 20.0%
!! TTAACGTTTGTTTAAGTATT+TGG - chr1.2:48895597-48895616 MS.gene029813:intron 20.0%
!! TTTATAGATTATTGTGTTTC+CGG - chr1.2:48896245-48896264 MS.gene029813:intron 20.0%
!!! AAGATAATAAAATTTTGCAC+TGG - chr1.2:48896774-48896793 MS.gene029813:intron 20.0%
!!! AGATTTTTAGCTGTTTTTAT+CGG - chr1.2:48896056-48896075 MS.gene029813:intron 20.0%
!!! CTATTTTTATCTCCATTAAA+TGG - chr1.2:48896985-48897004 MS.gene029813:intron 20.0%
!!! GTGTTGATTTGAATAATAAA+AGG - chr1.2:48895202-48895221 MS.gene029813:intron 20.0%
!!! TCTTTCTTTTTTGTTTTTTG+CGG - chr1.2:48895180-48895199 MS.gene029813:CDS 20.0%
!!! TGAGTTTAACTTATTTAGTT+TGG - chr1.2:48895935-48895954 MS.gene029813:CDS 20.0%
!!! TTCTTATTCGAAGTATTAAA+GGG - chr1.2:48896749-48896768 MS.gene029813:CDS 20.0%
!!! TTCTTTTCTTCTATTTTTGT+AGG - chr1.2:48897852-48897871 MS.gene029813:intron 20.0%
!!! TTTCTTATTCGAAGTATTAA+AGG - chr1.2:48896748-48896767 MS.gene029813:CDS 20.0%
!!! TTTGTTGTTGAATAATGTTT+TGG - chr1.2:48895405-48895424 MS.gene029813:CDS 20.0%
!!! TTTTTATTGCATCTAATTGT+AGG - chr1.2:48896862-48896881 MS.gene029813:intron 20.0%
! AACAGAGATCAAAACAAATA+AGG + chr1.2:48895690-48895709 None:intergenic 25.0%
! AATGATAAATTGACCTTGAA+TGG + chr1.2:48897809-48897828 None:intergenic 25.0%
! AATTAACTACAAAAAGCAGA+AGG + chr1.2:48897735-48897754 None:intergenic 25.0%
! ATCAAATACTTCTAGTAGAA+AGG + chr1.2:48897187-48897206 None:intergenic 25.0%
! ATGATAAATTGACCTTGAAT+GGG + chr1.2:48897808-48897827 None:intergenic 25.0%
! ATTAACTACAAAAAGCAGAA+GGG + chr1.2:48897734-48897753 None:intergenic 25.0%
! CACAACTACAAATATAACTT+TGG + chr1.2:48897763-48897782 None:intergenic 25.0%
! CTTTAGGTAATAGTATAACA+GGG + chr1.2:48897678-48897697 None:intergenic 25.0%
! GTATCAACTAATTTCTCTTT+AGG + chr1.2:48897694-48897713 None:intergenic 25.0%
! TAGATTAATATATACGGTGT+TGG - chr1.2:48895559-48895578 MS.gene029813:intron 25.0%
! TATACAACAAGATTGAAAGT+AGG - chr1.2:48895529-48895548 MS.gene029813:CDS 25.0%
! TCAAATACTTCTAGTAGAAA+GGG + chr1.2:48897186-48897205 None:intergenic 25.0%
! TCTTTAGGTAATAGTATAAC+AGG + chr1.2:48897679-48897698 None:intergenic 25.0%
! TTATAGATTATTGTGTTTCC+GGG - chr1.2:48896246-48896265 MS.gene029813:intron 25.0%
! TTCGAATAAGAAAAAATGCA+AGG + chr1.2:48896741-48896760 None:intergenic 25.0%
! TTTCATTTAGTAACTCATCT+TGG - chr1.2:48897499-48897518 MS.gene029813:intron 25.0%
!! TCTCATTTCAGATTCATTTT+CGG - chr1.2:48895356-48895375 MS.gene029813:intron 25.0%
!! TTAACAGTTTAGGATTCTTA+CGG + chr1.2:48897306-48897325 None:intergenic 25.0%
!!! TGGTTTTCAATTTGATTTAC+AGG - chr1.2:48895250-48895269 MS.gene029813:intron 25.0%
AATGTAAAAGCAAAACTGCA+AGG + chr1.2:48897481-48897500 None:intergenic 30.0%
ATGTAAAAGCAAAACTGCAA+GGG + chr1.2:48897480-48897499 None:intergenic 30.0%
CATAGATTTGTGCTTATCTT+CGG + chr1.2:48895307-48895326 None:intergenic 30.0%
GATGTACTCCACATTATATT+TGG + chr1.2:48896184-48896203 None:intergenic 30.0%
GATGTGATGTAATAATTGGT+TGG - chr1.2:48896004-48896023 MS.gene029813:CDS 30.0%
GCCAGCAATATAGAAATATT+TGG + chr1.2:48897071-48897090 None:intergenic 30.0%
GGAAAAAAAAATCCCATTCA+AGG - chr1.2:48897793-48897812 MS.gene029813:CDS 30.0%
TAAAACTGAATTCAACGGTT+CGG + chr1.2:48896604-48896623 None:intergenic 30.0%
TCATCAAGATGACTAACATT+TGG - chr1.2:48897011-48897030 MS.gene029813:intron 30.0%
! AACTAATTGAATGGATTCAG+GGG + chr1.2:48897110-48897129 None:intergenic 30.0%
! AGAACATTTTCCAAAGTTCA+TGG - chr1.2:48897421-48897440 MS.gene029813:intron 30.0%
! ATCTTTAAGGATTTGCATTC+TGG - chr1.2:48897354-48897373 MS.gene029813:intron 30.0%
! CAACTAATTGAATGGATTCA+GGG + chr1.2:48897111-48897130 None:intergenic 30.0%
! GAGAAATTAGTTGATACCAA+TGG - chr1.2:48897697-48897716 MS.gene029813:intron 30.0%
! GCCAAATATTTCTATATTGC+TGG - chr1.2:48897067-48897086 MS.gene029813:intron 30.0%
! TCTTGATGATTCCCATTTAA+TGG + chr1.2:48897000-48897019 None:intergenic 30.0%
! TCTTTAAGGATTTGCATTCT+GGG - chr1.2:48897355-48897374 MS.gene029813:intron 30.0%
!! AAAAATGTTACTTTCCTTCC+CGG + chr1.2:48896267-48896286 None:intergenic 30.0%
!! AGGTATATGGACTATGTTTT+TGG - chr1.2:48897382-48897401 MS.gene029813:intron 30.0%
!! TTTGATTTTGCTTCTTACAG+TGG - chr1.2:48896425-48896444 MS.gene029813:intron 30.0%
AAGGATTACTGTTATCACAC+AGG - chr1.2:48897606-48897625 MS.gene029813:intron 35.0%
AATGAAAATCAGGCTTCTCT+GGG + chr1.2:48897995-48898014 None:intergenic 35.0%
AATGGCTGCCAAATATAATG+TGG - chr1.2:48896173-48896192 MS.gene029813:CDS 35.0%
AATGTTATCATCCAAAGGTC+TGG - chr1.2:48897960-48897979 MS.gene029813:CDS 35.0%
AGGATTACTGTTATCACACA+GGG - chr1.2:48897607-48897626 MS.gene029813:intron 35.0%
ATACTTCTAGTAGAAAGGGA+AGG + chr1.2:48897182-48897201 None:intergenic 35.0%
ATTTATCTGCGAGTTCTTCA+AGG - chr1.2:48897260-48897279 MS.gene029813:intron 35.0%
CAGCAAATGTTATCATCCAA+AGG - chr1.2:48897955-48897974 MS.gene029813:CDS 35.0%
TAATGAAAATCAGGCTTCTC+TGG + chr1.2:48897996-48898015 None:intergenic 35.0%
TGAAAGAAATCCTCCAACAA+AGG + chr1.2:48897883-48897902 None:intergenic 35.0%
TGAATCAGATCTCATGTTTC+TGG - chr1.2:48895230-48895249 MS.gene029813:intron 35.0%
TGCTACTCAGAATTCAATCA+GGG - chr1.2:48896318-48896337 MS.gene029813:intron 35.0%
TGGAGGATTTCTTTCACAAT+TGG - chr1.2:48897887-48897906 MS.gene029813:intron 35.0%
TTCAACGTAATCACAAGTGT+TGG + chr1.2:48895771-48895790 None:intergenic 35.0%
TTGAAGAACTCGCAGATAAA+TGG + chr1.2:48897261-48897280 None:intergenic 35.0%
TTGTAGTTGTGCTTATGAGT+AGG - chr1.2:48897772-48897791 MS.gene029813:CDS 35.0%
! ACACCTTCGATCAAAAGTAT+TGG + chr1.2:48895716-48895735 None:intergenic 35.0%
! ACTAGTTACATTGGTTGCAT+CGG - chr1.2:48896469-48896488 MS.gene029813:intron 35.0%
! ATTTGAAGCAACCCGAAAAT+TGG - chr1.2:48896931-48896950 MS.gene029813:intron 35.0%
! ATTTTCCAAAGTTCATGGCT+GGG - chr1.2:48897426-48897445 MS.gene029813:intron 35.0%
! CTTGTGCTAGAATGCTATTT+AGG - chr1.2:48895643-48895662 MS.gene029813:CDS 35.0%
! GCAACTAATTGAATGGATTC+AGG + chr1.2:48897112-48897131 None:intergenic 35.0%
! TGTTTGAGTCTTTGAGAGTT+TGG - chr1.2:48895959-48895978 MS.gene029813:CDS 35.0%
! TTTGAAGCAACCCGAAAATT+GGG - chr1.2:48896932-48896951 MS.gene029813:intron 35.0%
! TTTTCCATCTCTCAGTTTTC+TGG - chr1.2:48896031-48896050 MS.gene029813:intron 35.0%
!! TCTAACCTTGTTATTTGCTG+CGG + chr1.2:48897143-48897162 None:intergenic 35.0%
!!! AATTTTCGGGTTGCTTCAAA+TGG + chr1.2:48896932-48896951 None:intergenic 35.0%
AAAGATATCCCAGTTGCCAA+AGG - chr1.2:48897567-48897586 MS.gene029813:intron 40.0%
ACCAAGAACTCTAAACAAGC+TGG - chr1.2:48896517-48896536 MS.gene029813:intron 40.0%
AGATGCTGAACAGATCAACA+AGG + chr1.2:48896967-48896986 None:intergenic 40.0%
AGATTATTGTGTTTCCGGGA+AGG - chr1.2:48896250-48896269 MS.gene029813:intron 40.0%
AGTATCTTACTCCCTTGTGA+AGG - chr1.2:48897212-48897231 MS.gene029813:intron 40.0%
ATCAGCTGCAAATTGCTACA+AGG - chr1.2:48896902-48896921 MS.gene029813:CDS 40.0%
ATGCTCGGCAACTAATTGAA+TGG + chr1.2:48897119-48897138 None:intergenic 40.0%
ATTGAAGAATGTGTGAGAGC+TGG - chr1.2:48897924-48897943 MS.gene029813:intron 40.0%
CAAGAATGGCATTGTTCAAC+TGG + chr1.2:48895284-48895303 None:intergenic 40.0%
CAGACAACCAAAATGAAGCA+CGG + chr1.2:48895853-48895872 None:intergenic 40.0%
CATCATGAAGACCTTCACAA+GGG + chr1.2:48897226-48897245 None:intergenic 40.0%
CTTTCCAGAAAACTGAGAGA+TGG + chr1.2:48896038-48896057 None:intergenic 40.0%
GAAGATGAGAATACACCTAC+AGG - chr1.2:48896664-48896683 MS.gene029813:intron 40.0%
GATGCAACCAATGTAACTAG+TGG + chr1.2:48896470-48896489 None:intergenic 40.0%
GCAATTTGCAGCTGATAAGT+TGG + chr1.2:48896898-48896917 None:intergenic 40.0%
GCTCTCACACATTCTTCAAT+AGG + chr1.2:48897924-48897943 None:intergenic 40.0%
TATAATGTGGAGTACATCGC+TGG - chr1.2:48896186-48896205 MS.gene029813:CDS 40.0%
TATGATCCTACGACTGTTTC+AGG - chr1.2:48896142-48896161 MS.gene029813:CDS 40.0%
TCATCATGAAGACCTTCACA+AGG + chr1.2:48897227-48897246 None:intergenic 40.0%
TGCATCGGAAAAGACAAGTT+TGG - chr1.2:48896484-48896503 MS.gene029813:intron 40.0%
TTGAAGAATGTGTGAGAGCT+GGG - chr1.2:48897925-48897944 MS.gene029813:intron 40.0%
TTTGTAGGAGATGCCTTTGT+TGG - chr1.2:48897867-48897886 MS.gene029813:intron 40.0%
! ACATTTTCAGTCTGGTCAAC+AGG + chr1.2:48897532-48897551 None:intergenic 40.0%
! ACCAGCTTGTTTAGAGTTCT+TGG + chr1.2:48896521-48896540 None:intergenic 40.0%
! CATTTTCCAAAGTTCATGGC+TGG - chr1.2:48897425-48897444 MS.gene029813:intron 40.0%
! CTGTTTCAGGTACGATGAAA+TGG - chr1.2:48896155-48896174 MS.gene029813:CDS 40.0%
! GCACCAATACTTTTGATCGA+AGG - chr1.2:48895710-48895729 MS.gene029813:intron 40.0%
! GGTACCTAATGCCCAATTTT+CGG + chr1.2:48896946-48896965 None:intergenic 40.0%
! GTACCTAATGCCCAATTTTC+GGG + chr1.2:48896945-48896964 None:intergenic 40.0%
! GTGCTACTCAGAATTCAATC+AGG - chr1.2:48896317-48896336 MS.gene029813:intron 40.0%
! GTGTTTGACACGAAAAGTGT+AGG + chr1.2:48895749-48895768 None:intergenic 40.0%
! TTAGTTGATACCAATGGAGC+AGG - chr1.2:48897703-48897722 MS.gene029813:intron 40.0%
!! CATTCTGGGATTCAGGTATA+TGG - chr1.2:48897369-48897388 MS.gene029813:intron 40.0%
ATCGTACCTGAAACAGTCGT+AGG + chr1.2:48896151-48896170 None:intergenic 45.0%
CAAACAGCACAAGTTCCTGT+AGG + chr1.2:48896682-48896701 None:intergenic 45.0%
CAACCCGAAAATTGGGCATT+AGG - chr1.2:48896939-48896958 MS.gene029813:intron 45.0%
CCATCTTGAGCAACACATAC+GGG + chr1.2:48897640-48897659 None:intergenic 45.0%
CCCATCTTGAGCAACACATA+CGG + chr1.2:48897641-48897660 None:intergenic 45.0%
CGCAGATAAATGGTGCAGAA+AGG + chr1.2:48897251-48897270 None:intergenic 45.0%
GAATTCAATCAGGGTTGCTC+AGG - chr1.2:48896327-48896346 MS.gene029813:intron 45.0%
GTGCTTATCTTCGGCAAGAA+TGG + chr1.2:48895298-48895317 None:intergenic 45.0%
TGACCAGACTGAAAATGTCG+AGG - chr1.2:48897534-48897553 MS.gene029813:intron 45.0%
TGGATTCAGGGGATACAGTA+AGG + chr1.2:48897099-48897118 None:intergenic 45.0%
! CACGAAAAGTGTAGGACATC+AGG + chr1.2:48895741-48895760 None:intergenic 45.0%
! CCGTATGTGTTGCTCAAGAT+GGG - chr1.2:48897638-48897657 MS.gene029813:intron 45.0%
! CGGAAAAGACAAGTTTGGTG+AGG - chr1.2:48896489-48896508 MS.gene029813:intron 45.0%
! TCTCCTCGACATTTTCAGTC+TGG + chr1.2:48897540-48897559 None:intergenic 45.0%
! TCTGTGTCCGTGCTTCATTT+TGG - chr1.2:48895843-48895862 MS.gene029813:intron 45.0%
!! ACTTGTGCTGTTTGTGTGGT+TGG - chr1.2:48896688-48896707 MS.gene029813:intron 45.0%
!! AGGAACTTGTGCTGTTTGTG+TGG - chr1.2:48896684-48896703 MS.gene029813:intron 45.0%
!! GAAGGTGTTCTTCTCGGTAT+GGG - chr1.2:48895082-48895101 MS.gene029813:CDS 45.0%
!! GCTTTGGAAGGTGTTCTTCT+CGG - chr1.2:48895076-48895095 MS.gene029813:CDS 45.0%
!! GGATTTGCATTCTGGGATTC+AGG - chr1.2:48897362-48897381 MS.gene029813:intron 45.0%
!! TTTGTGTGGTTGGTGGTGAA+AGG - chr1.2:48896698-48896717 MS.gene029813:intron 45.0%
AAGGGCAATACCTGCTCCAT+TGG + chr1.2:48897716-48897735 None:intergenic 50.0%
AATGTGGAGTACATCGCTGG+AGG - chr1.2:48896189-48896208 MS.gene029813:intron 50.0%
ACAGCAGAGATGTCGAGAAG+GGG + chr1.2:48895112-48895131 None:intergenic 50.0%
ACCAATGTAACTAGTGGCCC+CGG + chr1.2:48896464-48896483 None:intergenic 50.0%
CGAGTTCTTCAAGGATCCAC+AGG - chr1.2:48897269-48897288 MS.gene029813:intron 50.0%
CTCTGATGCCTTTGGCAACT+GGG + chr1.2:48897578-48897597 None:intergenic 50.0%
GACAGCAGAGATGTCGAGAA+GGG + chr1.2:48895113-48895132 None:intergenic 50.0%
GCATGCCGCAGCAAATAACA+AGG - chr1.2:48897135-48897154 MS.gene029813:intron 50.0%
GTAGGAGATGCCTTTGTTGG+AGG - chr1.2:48897870-48897889 MS.gene029813:intron 50.0%
TACCTCCCAGCCATGAACTT+TGG + chr1.2:48897434-48897453 None:intergenic 50.0%
TTCCAAAGTTCATGGCTGGG+AGG - chr1.2:48897429-48897448 MS.gene029813:intron 50.0%
! AGTGGATGCTTCAAGTTCCC+GGG - chr1.2:48896443-48896462 MS.gene029813:intron 50.0%
! CAGTGGATGCTTCAAGTTCC+CGG - chr1.2:48896442-48896461 MS.gene029813:intron 50.0%
! CCCGTATGTGTTGCTCAAGA+TGG - chr1.2:48897637-48897656 MS.gene029813:intron 50.0%
! GTTATTTGCTGCGGCATGCT+CGG + chr1.2:48897134-48897153 None:intergenic 50.0%
!! AACGGTTCGGTACAGTGTAG+TGG + chr1.2:48896591-48896610 None:intergenic 50.0%
!! GGAAGGTGTTCTTCTCGGTA+TGG - chr1.2:48895081-48895100 MS.gene029813:CDS 50.0%
!! GGTTGGTGGTGAAAGGTAAG+AGG - chr1.2:48896705-48896724 MS.gene029813:intron 50.0%
!! TGTGCTGTTTGTGTGGTTGG+TGG - chr1.2:48896691-48896710 MS.gene029813:intron 50.0%
CCAATGTAACTAGTGGCCCC+GGG + chr1.2:48896463-48896482 None:intergenic 55.0%
CCTCTGATGCCTTTGGCAAC+TGG + chr1.2:48897579-48897598 None:intergenic 55.0%
CGACAGCAGAGATGTCGAGA+AGG + chr1.2:48895114-48895133 None:intergenic 55.0%
GGTAAGTGCAGCCAGACCTT+TGG + chr1.2:48897974-48897993 None:intergenic 55.0%
TACGGCAGAGCTTTCTCCTG+TGG + chr1.2:48897288-48897307 None:intergenic 55.0%
TTGCGTGCCTCTGATGCCTT+TGG + chr1.2:48897586-48897605 None:intergenic 55.0%
! AGGCATCAGAGGCACGCAAA+AGG - chr1.2:48897587-48897606 MS.gene029813:intron 55.0%
! GTGGATGCTTCAAGTTCCCG+GGG - chr1.2:48896444-48896463 MS.gene029813:intron 55.0%
!! CCAGTTGCCAAAGGCATCAG+AGG - chr1.2:48897576-48897595 MS.gene029813:intron 55.0%
CCCGGGGCCACTAGTTACAT+TGG - chr1.2:48896460-48896479 MS.gene029813:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 48895073 48898016 48895073 ID=MS.gene029813
chr1.2 mRNA 48895073 48898016 48895073 ID=MS.gene029813.t1;Parent=MS.gene029813
chr1.2 exon 48897934 48898016 48897934 ID=MS.gene029813.t1.exon1;Parent=MS.gene029813.t1
chr1.2 CDS 48897934 48898016 48897934 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48897764 48897817 48897764 ID=MS.gene029813.t1.exon2;Parent=MS.gene029813.t1
chr1.2 CDS 48897764 48897817 48897764 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48896879 48896925 48896879 ID=MS.gene029813.t1.exon3;Parent=MS.gene029813.t1
chr1.2 CDS 48896879 48896925 48896879 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48896741 48896772 48896741 ID=MS.gene029813.t1.exon4;Parent=MS.gene029813.t1
chr1.2 CDS 48896741 48896772 48896741 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48896543 48896644 48896543 ID=MS.gene029813.t1.exon5;Parent=MS.gene029813.t1
chr1.2 CDS 48896543 48896644 48896543 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48896370 48896437 48896370 ID=MS.gene029813.t1.exon6;Parent=MS.gene029813.t1
chr1.2 CDS 48896370 48896437 48896370 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48896129 48896205 48896129 ID=MS.gene029813.t1.exon7;Parent=MS.gene029813.t1
chr1.2 CDS 48896129 48896205 48896129 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48895933 48896030 48895933 ID=MS.gene029813.t1.exon8;Parent=MS.gene029813.t1
chr1.2 CDS 48895933 48896030 48895933 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48895785 48895855 48895785 ID=MS.gene029813.t1.exon9;Parent=MS.gene029813.t1
chr1.2 CDS 48895785 48895855 48895785 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48895639 48895705 48895639 ID=MS.gene029813.t1.exon10;Parent=MS.gene029813.t1
chr1.2 CDS 48895639 48895705 48895639 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48895365 48895548 48895365 ID=MS.gene029813.t1.exon11;Parent=MS.gene029813.t1
chr1.2 CDS 48895365 48895548 48895365 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
chr1.2 exon 48895073 48895215 48895073 ID=MS.gene029813.t1.exon12;Parent=MS.gene029813.t1
chr1.2 CDS 48895073 48895215 48895073 ID=cds.MS.gene029813.t1;Parent=MS.gene029813.t1
Gene Sequence

>MS.gene029813

ATGGCTTTGGAAGGTGTTCTTCTCGGTATGGGTAATCCCCTTCTCGACATCTCTGCTGTCGTCGACGAAGATTTCTTGAAAAAGTACGTCGCTTCTTTCTTACTACATCTTTCTTTTTTGTTTTTTGCGGTGTTGATTTGAATAATAAAAGGTGAAATGAATCAGATCTCATGTTTCTGGTTTTCAATTTGATTTACAGGTTTGATATCCAGTTGAACAATGCCATTCTTGCCGAAGATAAGCACAAATCTATGTAAGATTTTCGACTCTACGCGTTGTTTTTTCTCATTTCAGATTCATTTTCGGATCAGTACGATATCAACTATCAACGTTTTGTTGTTGAATAATGTTTTGGTTGTTTGAGAGTTTAACTTATTTATTTCGAAGAAATGATGTTTACACATGTTAAATACGATGTCTTTGTTTTTTGTGTGTATTTTTTAAATATGCGTAAAATATACAACAAGATTGAAAGTAGGAGAGACATAGATTAATATATACGGTGTTGGTATTTTGATGTTTAATTAACGTTTGTTTAAGTATTTGGTAAACATGCTAATTTGAATTTATCTTGTGCTAGAATGCTATTTAGGATAAATATAATGTCGAGTTTACCTTATTTGTTTTGATCTCTGTTGCACCAATACTTTTGATCGAAGGTGTGTCCTGATGTCCTACACTTTTCGTGTCAAACACCAACACTTGTGATTACGTTGAATTGTCATTTTTTTCAAAATTTTATCGGTTTCTGTATCAGTGTCGTGTCTGCGTCTGTGTCCGTGCTTCATTTTGGTTGTCTGAGCTGTCATGTAACATGCTATTTTTATTTGATTTTGTGCTATAATGTATTTTAAGATAATCTTGAGTTTAACTTATTTAGTTTGGTTGTTTGAGTCTTTGAGAGTTTGGTAAACATCCTAATTAAAGATTTGATGTGATGTAATAATTGGTTGGAGATTTTTCCATCTCTCAGTTTTCTGGAAAGATTTTTAGCTGTTTTTATCGGCTCTATTCTTTTTGTTTTAGTATTTAGATATTTTATGAAAAGATATCATGTTTTGTGACTTCATATGATCCTACGACTGTTTCAGGTACGATGAAATGGCTGCCAAATATAATGTGGAGTACATCGCTGGAGGTAATTACACTAGATCTGAATTGTTGTGTTTTAATTTATAGATTATTGTGTTTCCGGGAAGGAAAGTAACATTTTTTAATGATGTTTATGATTTTTCTTTTTTCAGGTGCTACTCAGAATTCAATCAGGGTTGCTCAGGTTTGTGCAGTGTGTAATTTCTTTTATATTAGTTGGAGTTGAATCTCGAGTAGCTGTTGTAAAAATAGCTTATCATTTTGATTTTGCTTCTTACAGTGGATGCTTCAAGTTCCCGGGGCCACTAGTTACATTGGTTGCATCGGAAAAGACAAGTTTGGTGAGGAGATGACCAAGAACTCTAAACAAGCTGGTGTAAATGTAAGTTTAATCTTGTAATTTTGTCTTGTTTCTTTTTCAATCCACTACACTGTACCGAACCGTTGAATTCAGTTTTAAAATTAACTAAATTCATTTAACAACTAGGTTCACTATTATGAAGATGAGAATACACCTACAGGAACTTGTGCTGTTTGTGTGGTTGGTGGTGAAAGGTAAGAGGATCAAGCTTTCCTTGCATTTTTTCTTATTCGAAGTATTAAAGGGAAAAGATAATAAAATTTTGCACTGGCTTAAAATAGTATACATCTTCAGCTTAGTATATATTTTTTATTGCAAAATTGTTGTTTGAAGCCTTTTTTATTGCATCTAATTGTAGGTCACTTATTGCCAACTTATCAGCTGCAAATTGCTACAAGGTCGACCATTTGAAGCAACCCGAAAATTGGGCATTAGGTACCTTGTTGATCTGTTCAGCATCTATTTTTATCTCCATTAAATGGGAATCATCAAGATGACTAACATTTGGTTTTGTGCATTTTTTTAATAAATAGTTGAGAAGGCCAAATATTTCTATATTGCTGGCTTTTTCCTTACTGTATCCCCTGAATCCATTCAATTAGTTGCCGAGCATGCCGCAGCAAATAACAAGGTTAGATATGATTAAAACTTTGCCTTCCCTTTCTACTAGAAGTATTTGATACTCAAGTATCTTACTCCCTTGTGAAGGTCTTCATGATGAACCTTTCTGCACCATTTATCTGCGAGTTCTTCAAGGATCCACAGGAGAAAGCTCTGCCGTAAGAATCCTAAACTGTTAAATTCAAATAAATTATTTTTTTCTTATTGATCTTTAAGGATTTGCATTCTGGGATTCAGGTATATGGACTATGTTTTTGGAAATGAGACTGAAGCAAGAACATTTTCCAAAGTTCATGGCTGGGAGGTAAATCATTACTTTGTAGATCTGTACCCTTGCAGTTTTGCTTTTACATTTCATTTAGTAACTCATCTTGGTTCTTTTCCTGTTGACCAGACTGAAAATGTCGAGGAGATAGCTTTAAAGATATCCCAGTTGCCAAAGGCATCAGAGGCACGCAAAAGGATTACTGTTATCACACAGGGTGCTGATCCCGTATGTGTTGCTCAAGATGGGAAAGTGACATTGTACCCTGTTATACTATTACCTAAAGAGAAATTAGTTGATACCAATGGAGCAGGTATTGCCCTTCTGCTTTTTGTAGTTAATTGTTGACCAAAGTTATATTTGTAGTTGTGCTTATGAGTAGGAAAAAAAAATCCCATTCAAGGTCAATTTATCATTGATGTCACATGCTAGTCGTAATATTCTTTTCTTCTATTTTTGTAGGAGATGCCTTTGTTGGAGGATTTCTTTCACAATTGGTTCAAGAGAAGCCTATTGAAGAATGTGTGAGAGCTGGGTGTTATGCAGCAAATGTTATCATCCAAAGGTCTGGCTGCACTTACCCAGAGAAGCCTGATTTTCATTAA
Protein sequence

>MS.gene029813.t1

MALEGVLLGMGNPLLDISAVVDEDFLKKFDIQLNNAILAEDKHKSMYDEMAAKYNVEYIAGGATQNSIRVAQWMLQVPGATSYIGCIGKDKFGEEMTKNSKQAGVNVHYYEDENTPTGTCAVCVVGGERSLIANLSAANCYKVDHLKQPENWALVEKAKYFYIAGFFLTVSPESIQLVAEHAAANNKVFMMNLSAPFICEFFKDPQEKALPYMDYVFGNETEARTFSKVHGWETENVEEIALKISQLPKASEARKRITVITQGADPVCVAQDGKVTLYPVILLPKEKLVDTNGAGDAFVGGFLSQLVQEKPIEECVRAGCYAANVIIQRSGCTYPEKPDFH