Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031139.t1 | XP_003618625.1 | 100 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 1.30E-59 | 238.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031139.t1 | Q9MAB9 | 56.4 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.1e-34 | 146.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031139.t1 | G7KLT7 | 100.0 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 9.1e-60 | 238.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97583 | MS.gene031139 | PPI |
| MS.gene031139 | MS.gene86087 | PPI |
| MS.gene031139 | MS.gene057527 | PPI |
| MS.gene031139 | MS.gene45247 | PPI |
| MS.gene031139 | MS.gene021818 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031139.t1 | MTR_6g013820 | 100.000 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 1.45e-82 | 237 |
| MS.gene031139.t1 | MTR_7g012560 | 75.630 | 119 | 27 | 1 | 1 | 117 | 1 | 119 | 5.33e-60 | 180 |
| MS.gene031139.t1 | MTR_7g012560 | 78.218 | 101 | 20 | 1 | 1 | 99 | 1 | 101 | 1.21e-51 | 159 |
| MS.gene031139.t1 | MTR_7g027300 | 53.097 | 113 | 49 | 2 | 8 | 117 | 7 | 118 | 1.27e-34 | 115 |
| MS.gene031139.t1 | MTR_8g096380 | 48.718 | 117 | 56 | 2 | 4 | 117 | 3 | 118 | 1.89e-33 | 113 |
| MS.gene031139.t1 | MTR_7g027017 | 48.246 | 114 | 51 | 1 | 8 | 113 | 7 | 120 | 5.68e-33 | 117 |
| MS.gene031139.t1 | MTR_7g108180 | 41.322 | 121 | 66 | 4 | 1 | 117 | 1 | 120 | 9.82e-26 | 93.6 |
| MS.gene031139.t1 | MTR_8g096390 | 44.444 | 90 | 48 | 1 | 3 | 90 | 2 | 91 | 2.57e-23 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene031139.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene031139.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene031139.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene031139.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene031139.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene031139.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene031139.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene031139.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene031139.t1 | AT1G22050 | 52.941 | 119 | 54 | 1 | 1 | 117 | 1 | 119 | 4.54e-43 | 137 |
| MS.gene031139.t1 | AT1G77870 | 52.101 | 119 | 53 | 2 | 3 | 117 | 2 | 120 | 1.43e-36 | 121 |
| MS.gene031139.t1 | AT3G26980 | 47.458 | 118 | 58 | 2 | 4 | 117 | 3 | 120 | 1.08e-32 | 111 |
| MS.gene031139.t1 | AT4G24990 | 48.718 | 117 | 56 | 3 | 4 | 117 | 3 | 118 | 8.97e-32 | 108 |
| MS.gene031139.t1 | AT4G24990 | 48.718 | 117 | 56 | 3 | 4 | 117 | 3 | 118 | 8.97e-32 | 108 |
| MS.gene031139.t1 | AT5G15460 | 52.381 | 84 | 37 | 1 | 37 | 117 | 11 | 94 | 4.15e-19 | 76.3 |
Find 18 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGTGCAACCACCTAATT+TGG | 0.182555 | 6.2:-8924475 | MS.gene031139:CDS |
| TACAAACCTTTGTCCAAATT+AGG | 0.290205 | 6.2:+8924462 | None:intergenic |
| ATCAATTAGAGATCAAATTT+CGG | 0.354770 | 6.2:-8925510 | MS.gene031139:CDS |
| AGTCCATTGTGTAATCTTCC+TGG | 0.429392 | 6.2:-8924519 | MS.gene031139:CDS |
| TGTATCTCTCTGAGTATGGC+TGG | 0.438310 | 6.2:-8925541 | None:intergenic |
| TTGAGCGATGGATCGGATAT+TGG | 0.459581 | 6.2:-8925487 | MS.gene031139:CDS |
| AAGAAAGTATTCTTACTCAA+TGG | 0.476431 | 6.2:-8925423 | MS.gene031139:CDS |
| GATGTGAAGTTAATTTGTGC+TGG | 0.476748 | 6.2:-8924582 | MS.gene031139:CDS |
| ATCAAATTTCGGTTGAGCGA+TGG | 0.478567 | 6.2:-8925499 | MS.gene031139:CDS |
| ATTCTTACTCAATGGCCAAA+AGG | 0.495557 | 6.2:-8925415 | MS.gene031139:intron |
| ATTTCGGTTGAGCGATGGAT+CGG | 0.507983 | 6.2:-8925494 | MS.gene031139:CDS |
| GTATCTCTCTGAGTATGGCT+GGG | 0.571681 | 6.2:-8925540 | None:intergenic |
| CAACCACCTAATTTGGACAA+AGG | 0.572921 | 6.2:-8924468 | MS.gene031139:intron |
| AAACCTTTGTCCAAATTAGG+TGG | 0.589222 | 6.2:+8924465 | None:intergenic |
| AGCATTGTTGTAACTCCACC+AGG | 0.610313 | 6.2:+8924501 | None:intergenic |
| CCATTGTGTAATCTTCCTGG+TGG | 0.622842 | 6.2:-8924516 | MS.gene031139:CDS |
| GGAGAACAACAAAACAGTAG+AGG | 0.643312 | 6.2:-8924550 | MS.gene031139:CDS |
| CCACCAGGAAGATTACACAA+TGG | 0.747125 | 6.2:+8924516 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTCAAATGTTTATATTAA+TGG | - | chr6.2:8924719-8924738 | MS.gene031139:intron | 15.0% |
| !! | GCAAAAAAAAAAAAAAAAAG+AGG | + | chr6.2:8924485-8924504 | None:intergenic | 15.0% |
| !!! | ATTTTTAGTTCATATAGTTT+TGG | - | chr6.2:8925421-8925440 | MS.gene031139:CDS | 15.0% |
| !! | ATCAATTAGAGATCAAATTT+CGG | - | chr6.2:8924101-8924120 | MS.gene031139:CDS | 20.0% |
| !! | ATTGAATTACATGGAAAATT+AGG | - | chr6.2:8924589-8924608 | MS.gene031139:CDS | 20.0% |
| !! | GAAAATTAGGAATATTAATG+TGG | - | chr6.2:8924602-8924621 | MS.gene031139:CDS | 20.0% |
| !! | GTTAAATGAATTGAATTACA+TGG | - | chr6.2:8924580-8924599 | MS.gene031139:CDS | 20.0% |
| !!! | ACATTTTTAGACTTTATAGT+TGG | + | chr6.2:8924919-8924938 | None:intergenic | 20.0% |
| !!! | ATATTTCCTTTTTGAAGATA+AGG | - | chr6.2:8924983-8925002 | MS.gene031139:intron | 20.0% |
| !!! | CATGTTTTAAAAGCTATTAT+TGG | - | chr6.2:8925221-8925240 | MS.gene031139:intron | 20.0% |
| !!! | GTTATTTTCATAAACTATCT+TGG | + | chr6.2:8924372-8924391 | None:intergenic | 20.0% |
| !!! | TGGTTGTTTAATCTTATTTA+GGG | - | chr6.2:8924220-8924239 | MS.gene031139:intron | 20.0% |
| !!! | TTGGTTGTTTAATCTTATTT+AGG | - | chr6.2:8924219-8924238 | MS.gene031139:intron | 20.0% |
| !!! | TTTAATTCTCATTGATTTTC+TGG | - | chr6.2:8925451-8925470 | MS.gene031139:CDS | 20.0% |
| ! | AATTTGTGCTGGAAAAATAT+TGG | - | chr6.2:8925040-8925059 | MS.gene031139:intron | 25.0% |
| ! | ACTTATTTGAGCTTATTTAC+TGG | - | chr6.2:8924256-8924275 | MS.gene031139:intron | 25.0% |
| ! | ATTGGTATCTTAGTATTCAT+AGG | + | chr6.2:8924511-8924530 | None:intergenic | 25.0% |
| ! | TAATGGTTTAACATTGTATG+AGG | - | chr6.2:8924736-8924755 | MS.gene031139:intron | 25.0% |
| ! | TACTGCAAATTAATAAGAAC+TGG | - | chr6.2:8924555-8924574 | MS.gene031139:CDS | 25.0% |
| ! | TTCAATGTAATCACAATTGT+CGG | - | chr6.2:8924834-8924853 | MS.gene031139:intron | 25.0% |
| !! | AATCTTATTTAGGGTTTGTT+TGG | - | chr6.2:8924229-8924248 | MS.gene031139:intron | 25.0% |
| !! | TCATGTTCTCAGAATTTTAT+CGG | + | chr6.2:8924809-8924828 | None:intergenic | 25.0% |
| !! | TTCACATCTTTTACAGTTTT+TGG | + | chr6.2:8925017-8925036 | None:intergenic | 25.0% |
| !! | TTTTAACACACTATAATTGC+AGG | + | chr6.2:8925256-8925275 | None:intergenic | 25.0% |
| !!! | AAGAAAGTATTCTTACTCAA+TGG | - | chr6.2:8924188-8924207 | MS.gene031139:intron | 25.0% |
| AAACTATCTTGGAGAGATTA+TGG | + | chr6.2:8924361-8924380 | None:intergenic | 30.0% | |
| AAATACATTGTTACGAGAGT+TGG | + | chr6.2:8925388-8925407 | None:intergenic | 30.0% | |
| CATACTCCTTATCTTCAAAA+AGG | + | chr6.2:8924992-8925011 | None:intergenic | 30.0% | |
| GATGATTCCATTTAAGTGTA+TGG | - | chr6.2:8924670-8924689 | MS.gene031139:intron | 30.0% | |
| GTATCTTAGTATTCATAGGA+AGG | + | chr6.2:8924507-8924526 | None:intergenic | 30.0% | |
| GTTACAACCATACACTTAAA+TGG | + | chr6.2:8924680-8924699 | None:intergenic | 30.0% | |
| TACAAACCTTTGTCCAAATT+AGG | + | chr6.2:8925152-8925171 | None:intergenic | 30.0% | |
| ! | AGATTAAACAACCAACCTTT+TGG | + | chr6.2:8924214-8924233 | None:intergenic | 30.0% |
| !! | CTTTTTGAAGATAAGGAGTA+TGG | - | chr6.2:8924990-8925009 | MS.gene031139:intron | 30.0% |
| !! | TGGATGTATTGATTGATAGA+AGG | - | chr6.2:8924622-8924641 | MS.gene031139:intron | 30.0% |
| !!! | TTATTTTTTTGAGGGAGCAA+AGG | - | chr6.2:8925179-8925198 | MS.gene031139:intron | 30.0% |
| AAACCTTTGTCCAAATTAGG+TGG | + | chr6.2:8925149-8925168 | None:intergenic | 35.0% | |
| ATTCTTACTCAATGGCCAAA+AGG | - | chr6.2:8924196-8924215 | MS.gene031139:intron | 35.0% | |
| TCATTACAACAGACACAGAT+TGG | + | chr6.2:8924529-8924548 | None:intergenic | 35.0% | |
| !! | GATGTGAAGTTAATTTGTGC+TGG | - | chr6.2:8925029-8925048 | MS.gene031139:intron | 35.0% |
| AGTCCATTGTGTAATCTTCC+TGG | - | chr6.2:8925092-8925111 | MS.gene031139:intron | 40.0% | |
| AGTTGTGCAACCACCTAATT+TGG | - | chr6.2:8925136-8925155 | MS.gene031139:intron | 40.0% | |
| ATCAAATTTCGGTTGAGCGA+TGG | - | chr6.2:8924112-8924131 | MS.gene031139:CDS | 40.0% | |
| ATTCCTAGAGTCGCTCATTT+GGG | + | chr6.2:8925326-8925345 | None:intergenic | 40.0% | |
| CAACCACCTAATTTGGACAA+AGG | - | chr6.2:8925143-8925162 | MS.gene031139:intron | 40.0% | |
| GGAGAACAACAAAACAGTAG+AGG | - | chr6.2:8925061-8925080 | MS.gene031139:intron | 40.0% | |
| GTGTGTTAAAATGTGTGCGT+TGG | - | chr6.2:8925265-8925284 | MS.gene031139:intron | 40.0% | |
| TTACTCAATGGCCAAAAGGT+TGG | - | chr6.2:8924200-8924219 | MS.gene031139:intron | 40.0% | |
| ! | ACACCTTCAATCAGAAGTGT+CGG | - | chr6.2:8924884-8924903 | MS.gene031139:intron | 40.0% |
| ! | ACTTACTGGTTGTTTCACAC+GGG | - | chr6.2:8925289-8925308 | MS.gene031139:intron | 40.0% |
| ! | CACTTACTGGTTGTTTCACA+CGG | - | chr6.2:8925288-8925307 | MS.gene031139:intron | 40.0% |
| ! | GTAGCAGCAGCAAAACTTTT+CGG | + | chr6.2:8924151-8924170 | None:intergenic | 40.0% |
| ! | TAGCAGCAGCAAAACTTTTC+GGG | + | chr6.2:8924150-8924169 | None:intergenic | 40.0% |
| AGCATTGTTGTAACTCCACC+AGG | + | chr6.2:8925113-8925132 | None:intergenic | 45.0% | |
| ATTTCGGTTGAGCGATGGAT+CGG | - | chr6.2:8924117-8924136 | MS.gene031139:CDS | 45.0% | |
| CCACCAGGAAGATTACACAA+TGG | + | chr6.2:8925098-8925117 | None:intergenic | 45.0% | |
| CCATTGTGTAATCTTCCTGG+TGG | - | chr6.2:8925095-8925114 | MS.gene031139:intron | 45.0% | |
| GAACCGACACTTCTGATTGA+AGG | + | chr6.2:8924890-8924909 | None:intergenic | 45.0% | |
| GATTCCTAGAGTCGCTCATT+TGG | + | chr6.2:8925327-8925346 | None:intergenic | 45.0% | |
| TTGAGCGATGGATCGGATAT+TGG | - | chr6.2:8924124-8924143 | MS.gene031139:intron | 45.0% | |
| ! | TTCTGATTGAAGGTGTGTCC+TGG | + | chr6.2:8924880-8924899 | None:intergenic | 45.0% |
| !!! | ATTTTTTTTTATTTTTTTGA+GGG | - | chr6.2:8925171-8925190 | MS.gene031139:intron | 5.0% |
| !!! | TATTTTTTTTTATTTTTTTG+AGG | - | chr6.2:8925170-8925189 | MS.gene031139:intron | 5.0% |
| CAACCCAAATGAGCGACTCT+AGG | - | chr6.2:8925320-8925339 | MS.gene031139:intron | 50.0% | |
| ! | ACTGGTTGTTTCACACGGGT+TGG | - | chr6.2:8925293-8925312 | MS.gene031139:intron | 50.0% |
| ! | ATGTGTGCGTTGGCACTTAC+TGG | - | chr6.2:8925275-8925294 | MS.gene031139:intron | 50.0% |
| CGACGTGTCAATGTCGTGTC+TGG | + | chr6.2:8924783-8924802 | None:intergenic | 55.0% | |
| !! | CACAATTGTCGGTGCCGTGT+AGG | - | chr6.2:8924845-8924864 | MS.gene031139:intron | 55.0% |
| CCTGGTGTCCGACACCTACA+CGG | + | chr6.2:8924862-8924881 | None:intergenic | 60.0% | |
| !! | TGTCGGTGCCGTGTAGGTGT+CGG | - | chr6.2:8924851-8924870 | MS.gene031139:intron | 60.0% |
| !! | CCGTGTAGGTGTCGGACACC+AGG | - | chr6.2:8924859-8924878 | MS.gene031139:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 8924085 | 8925548 | 8924085 | ID=MS.gene031139 |
| chr6.2 | mRNA | 8924085 | 8925548 | 8924085 | ID=MS.gene031139.t1;Parent=MS.gene031139 |
| chr6.2 | exon | 8925416 | 8925548 | 8925416 | ID=MS.gene031139.t1.exon1;Parent=MS.gene031139.t1 |
| chr6.2 | CDS | 8925416 | 8925548 | 8925416 | ID=cds.MS.gene031139.t1;Parent=MS.gene031139.t1 |
| chr6.2 | exon | 8924469 | 8924633 | 8924469 | ID=MS.gene031139.t1.exon2;Parent=MS.gene031139.t1 |
| chr6.2 | CDS | 8924469 | 8924633 | 8924469 | ID=cds.MS.gene031139.t1;Parent=MS.gene031139.t1 |
| chr6.2 | exon | 8924085 | 8924140 | 8924085 | ID=MS.gene031139.t1.exon3;Parent=MS.gene031139.t1 |
| chr6.2 | CDS | 8924085 | 8924140 | 8924085 | ID=cds.MS.gene031139.t1;Parent=MS.gene031139.t1 |
| Gene Sequence |
| Protein sequence |
