Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86087.t1 | XP_003618625.1 | 100 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 1.30E-59 | 238.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86087.t1 | Q9MAB9 | 56.4 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.1e-34 | 146.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86087.t1 | G7KLT7 | 100.0 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 9.1e-60 | 238.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene021818 | MS.gene86087 | PPI |
| MS.gene97583 | MS.gene86087 | PPI |
| MS.gene45247 | MS.gene86087 | PPI |
| MS.gene057527 | MS.gene86087 | PPI |
| MS.gene031139 | MS.gene86087 | PPI |
| MS.gene86087 | MS.gene057527 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86087.t1 | MTR_6g013820 | 100.000 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 1.45e-82 | 237 |
| MS.gene86087.t1 | MTR_7g012560 | 75.630 | 119 | 27 | 1 | 1 | 117 | 1 | 119 | 5.33e-60 | 180 |
| MS.gene86087.t1 | MTR_7g012560 | 78.218 | 101 | 20 | 1 | 1 | 99 | 1 | 101 | 1.21e-51 | 159 |
| MS.gene86087.t1 | MTR_7g027300 | 53.097 | 113 | 49 | 2 | 8 | 117 | 7 | 118 | 1.27e-34 | 115 |
| MS.gene86087.t1 | MTR_8g096380 | 48.718 | 117 | 56 | 2 | 4 | 117 | 3 | 118 | 1.89e-33 | 113 |
| MS.gene86087.t1 | MTR_7g027017 | 48.246 | 114 | 51 | 1 | 8 | 113 | 7 | 120 | 5.68e-33 | 117 |
| MS.gene86087.t1 | MTR_7g108180 | 41.322 | 121 | 66 | 4 | 1 | 117 | 1 | 120 | 9.82e-26 | 93.6 |
| MS.gene86087.t1 | MTR_8g096390 | 44.444 | 90 | 48 | 1 | 3 | 90 | 2 | 91 | 2.57e-23 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86087.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene86087.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene86087.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene86087.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene86087.t1 | AT3G01050 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 2.54e-46 | 145 |
| MS.gene86087.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene86087.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene86087.t1 | AT5G15460 | 61.864 | 118 | 42 | 1 | 1 | 115 | 1 | 118 | 8.68e-44 | 139 |
| MS.gene86087.t1 | AT1G22050 | 52.941 | 119 | 54 | 1 | 1 | 117 | 1 | 119 | 4.54e-43 | 137 |
| MS.gene86087.t1 | AT1G77870 | 52.101 | 119 | 53 | 2 | 3 | 117 | 2 | 120 | 1.43e-36 | 121 |
| MS.gene86087.t1 | AT3G26980 | 47.458 | 118 | 58 | 2 | 4 | 117 | 3 | 120 | 1.08e-32 | 111 |
| MS.gene86087.t1 | AT4G24990 | 48.718 | 117 | 56 | 3 | 4 | 117 | 3 | 118 | 8.97e-32 | 108 |
| MS.gene86087.t1 | AT4G24990 | 48.718 | 117 | 56 | 3 | 4 | 117 | 3 | 118 | 8.97e-32 | 108 |
| MS.gene86087.t1 | AT5G15460 | 52.381 | 84 | 37 | 1 | 37 | 117 | 11 | 94 | 4.15e-19 | 76.3 |
Find 18 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGTGCAACCACCTAATT+TGG | 0.182555 | 6.3:-8629949 | MS.gene86087:CDS |
| TACAAACCTTTGTCCAAATT+AGG | 0.290205 | 6.3:+8629936 | None:intergenic |
| ATCAATTAGAGATCAAATTT+CGG | 0.354770 | 6.3:-8630982 | MS.gene86087:CDS |
| AGTCCATTGTGTAATCTTCC+TGG | 0.429392 | 6.3:-8629993 | MS.gene86087:CDS |
| TCTGTTTCTCTGAGTATGGC+TGG | 0.457554 | 6.3:-8631013 | None:intergenic |
| TTGAGCGATGGATCGGATAT+TGG | 0.459581 | 6.3:-8630959 | MS.gene86087:CDS |
| AAGAAAGTATTCTTACTCAA+TGG | 0.476431 | 6.3:-8630895 | MS.gene86087:CDS |
| GATGTGAAGTTAATTTGTGC+TGG | 0.476748 | 6.3:-8630056 | MS.gene86087:CDS |
| ATCAAATTTCGGTTGAGCGA+TGG | 0.478567 | 6.3:-8630971 | MS.gene86087:CDS |
| ATTCTTACTCAATGGCCAAA+AGG | 0.495557 | 6.3:-8630887 | MS.gene86087:intron |
| ATTTCGGTTGAGCGATGGAT+CGG | 0.507983 | 6.3:-8630966 | MS.gene86087:CDS |
| CTGTTTCTCTGAGTATGGCT+GGG | 0.558929 | 6.3:-8631012 | None:intergenic |
| CAACCACCTAATTTGGACAA+AGG | 0.572921 | 6.3:-8629942 | MS.gene86087:intron |
| AAACCTTTGTCCAAATTAGG+TGG | 0.589222 | 6.3:+8629939 | None:intergenic |
| AGCATTGTTGTAACTCCACC+AGG | 0.610313 | 6.3:+8629975 | None:intergenic |
| CCATTGTGTAATCTTCCTGG+TGG | 0.622842 | 6.3:-8629990 | MS.gene86087:CDS |
| GGAGAACAACAAAACAGTAG+AGG | 0.643312 | 6.3:-8630024 | MS.gene86087:CDS |
| CCACCAGGAAGATTACACAA+TGG | 0.747125 | 6.3:+8629990 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAATGTTTATATTAATAA+TGG | - | chr6.3:8630198-8630217 | MS.gene86087:intron | 10.0% |
| !! | TTCAATATAATCACAATTAT+CGG | - | chr6.3:8630312-8630331 | MS.gene86087:intron | 15.0% |
| !!! | ACATTTTAATTCTTTATAGT+TGG | + | chr6.3:8630397-8630416 | None:intergenic | 15.0% |
| !!! | ATTTTTAGTTCATATAGTTT+TGG | - | chr6.3:8630893-8630912 | MS.gene86087:CDS | 15.0% |
| !!! | GTTTGTATTTTTTTTTTTGA+GGG | - | chr6.3:8630643-8630662 | MS.gene86087:intron | 15.0% |
| !!! | TAGTTGTTTAATCTTATTTA+GGG | - | chr6.3:8629700-8629719 | MS.gene86087:intron | 15.0% |
| !!! | TTAGTTGTTTAATCTTATTT+AGG | - | chr6.3:8629699-8629718 | MS.gene86087:intron | 15.0% |
| !! | ATCAATTAGAGATCAAATTT+CGG | - | chr6.3:8629581-8629600 | MS.gene86087:CDS | 20.0% |
| !!! | CATGTTTTAAAAGCTATTAT+TGG | - | chr6.3:8630693-8630712 | MS.gene86087:intron | 20.0% |
| !!! | GGTTTGTATTTTTTTTTTTG+AGG | - | chr6.3:8630642-8630661 | MS.gene86087:intron | 20.0% |
| !!! | TCATGTTCTCATAATTTTAT+CGG | + | chr6.3:8630287-8630306 | None:intergenic | 20.0% |
| !!! | TTTAATTCTCATTGATTTTC+TGG | - | chr6.3:8630923-8630942 | MS.gene86087:CDS | 20.0% |
| !!! | TTTTTTGAAGATAAAGAGTA+TGG | - | chr6.3:8630468-8630487 | MS.gene86087:intron | 20.0% |
| ! | AATTTGTGCTGGAAAAATAT+TGG | - | chr6.3:8630518-8630537 | MS.gene86087:intron | 25.0% |
| ! | ATAATGGTTAACATTGTATG+AGG | - | chr6.3:8630214-8630233 | MS.gene86087:intron | 25.0% |
| ! | ATTGGTATCTTAGTATTCAT+AGG | + | chr6.3:8629981-8630000 | None:intergenic | 25.0% |
| ! | GAAAACTAGGAATATTAATG+TGG | - | chr6.3:8630072-8630091 | MS.gene86087:CDS | 25.0% |
| ! | GTTAAATGAATTGAGTAACA+TGG | - | chr6.3:8630050-8630069 | MS.gene86087:CDS | 25.0% |
| !! | AATCTTATTTAGGGTTTGTT+TGG | - | chr6.3:8629709-8629728 | MS.gene86087:intron | 25.0% |
| !! | AGATTAAACAACTAACCTTT+TGG | + | chr6.3:8629694-8629713 | None:intergenic | 25.0% |
| !! | GATGATTCCATTTAAGTTTA+TGG | - | chr6.3:8630140-8630159 | MS.gene86087:intron | 25.0% |
| !! | TTCACATCTTTTACAGTTTT+TGG | + | chr6.3:8630495-8630514 | None:intergenic | 25.0% |
| !! | TTTTAACACACTATAATTGC+AGG | + | chr6.3:8630728-8630747 | None:intergenic | 25.0% |
| !!! | AAGAAAGTATTCTTACTCAA+TGG | - | chr6.3:8629668-8629687 | MS.gene86087:intron | 25.0% |
| !!! | GTTATTTTCATAAGCTATCT+TGG | + | chr6.3:8629852-8629871 | None:intergenic | 25.0% |
| ATTGAGTAACATGGAAAACT+AGG | - | chr6.3:8630059-8630078 | MS.gene86087:CDS | 30.0% | |
| GTTACAGCCATAAACTTAAA+TGG | + | chr6.3:8630150-8630169 | None:intergenic | 30.0% | |
| TACAAACCTTTGTCCAAATT+AGG | + | chr6.3:8630630-8630649 | None:intergenic | 30.0% | |
| TACTGCAAATTAAGAAGAAC+TGG | - | chr6.3:8630025-8630044 | MS.gene86087:CDS | 30.0% | |
| TCATTACAACAGACACAAAT+TGG | + | chr6.3:8629999-8630018 | None:intergenic | 30.0% | |
| !!! | TTTTTTTTTTGAGGGAGCAA+AGG | - | chr6.3:8630651-8630670 | MS.gene86087:intron | 30.0% |
| AAACCTTTGTCCAAATTAGG+TGG | + | chr6.3:8630627-8630646 | None:intergenic | 35.0% | |
| AAGCTATCTTGGAGATCTTA+TGG | + | chr6.3:8629841-8629860 | None:intergenic | 35.0% | |
| ATTCTTACTCAATGGCCAAA+AGG | - | chr6.3:8629676-8629695 | MS.gene86087:intron | 35.0% | |
| GAATACATTGTTACCAGAGT+TGG | + | chr6.3:8630860-8630879 | None:intergenic | 35.0% | |
| TGGATGCATTCATTGATAGA+AGG | - | chr6.3:8630092-8630111 | MS.gene86087:intron | 35.0% | |
| !! | GATGTGAAGTTAATTTGTGC+TGG | - | chr6.3:8630507-8630526 | MS.gene86087:intron | 35.0% |
| !! | TGTTCTCTGATGCTTTTTAG+TGG | - | chr6.3:8630414-8630433 | MS.gene86087:intron | 35.0% |
| AGTCCATTGTGTAATCTTCC+TGG | - | chr6.3:8630570-8630589 | MS.gene86087:intron | 40.0% | |
| AGTTGTGCAACCACCTAATT+TGG | - | chr6.3:8630614-8630633 | MS.gene86087:intron | 40.0% | |
| ATCAAATTTCGGTTGAGCGA+TGG | - | chr6.3:8629592-8629611 | MS.gene86087:CDS | 40.0% | |
| ATTCCTAGAGTCGCTCATTT+GGG | + | chr6.3:8630798-8630817 | None:intergenic | 40.0% | |
| CAACCACCTAATTTGGACAA+AGG | - | chr6.3:8630621-8630640 | MS.gene86087:intron | 40.0% | |
| GGAGAACAACAAAACAGTAG+AGG | - | chr6.3:8630539-8630558 | MS.gene86087:intron | 40.0% | |
| GTGTGTTAAAATGTGTGCGT+TGG | - | chr6.3:8630737-8630756 | MS.gene86087:intron | 40.0% | |
| TCATAGGAAGACAGAAAGAG+AGG | + | chr6.3:8629965-8629984 | None:intergenic | 40.0% | |
| TGAACTACGAACACAGATAC+TGG | - | chr6.3:8630239-8630258 | MS.gene86087:intron | 40.0% | |
| ! | ACTTACTGGTTGTTTCACAC+GGG | - | chr6.3:8630761-8630780 | MS.gene86087:intron | 40.0% |
| ! | CACTTACTGGTTGTTTCACA+CGG | - | chr6.3:8630760-8630779 | MS.gene86087:intron | 40.0% |
| ! | GTAGCAGCAGCAAAACTTTT+CGG | + | chr6.3:8629631-8629650 | None:intergenic | 40.0% |
| ! | TAGCAGCAGCAAAACTTTTC+GGG | + | chr6.3:8629630-8629649 | None:intergenic | 40.0% |
| !! | GCACTGACACTTTTGATTGA+AGG | + | chr6.3:8630368-8630387 | None:intergenic | 40.0% |
| AGCATTGTTGTAACTCCACC+AGG | + | chr6.3:8630591-8630610 | None:intergenic | 45.0% | |
| ATTTCGGTTGAGCGATGGAT+CGG | - | chr6.3:8629597-8629616 | MS.gene86087:CDS | 45.0% | |
| CCACCAGGAAGATTACACAA+TGG | + | chr6.3:8630576-8630595 | None:intergenic | 45.0% | |
| CCATTGTGTAATCTTCCTGG+TGG | - | chr6.3:8630573-8630592 | MS.gene86087:intron | 45.0% | |
| GAGAGTCATGTTACCAACTC+TGG | - | chr6.3:8630844-8630863 | MS.gene86087:intron | 45.0% | |
| GATTCCTAGAGTCGCTCATT+TGG | + | chr6.3:8630799-8630818 | None:intergenic | 45.0% | |
| TTGAGCGATGGATCGGATAT+TGG | - | chr6.3:8629604-8629623 | MS.gene86087:intron | 45.0% | |
| !! | CACAATTATCGGTGTCGTGT+AGG | - | chr6.3:8630323-8630342 | MS.gene86087:intron | 45.0% |
| !! | TTTTGATTGAAGGCGTGTCC+TGG | + | chr6.3:8630358-8630377 | None:intergenic | 45.0% |
| CAACCCAAATGAGCGACTCT+AGG | - | chr6.3:8630792-8630811 | MS.gene86087:intron | 50.0% | |
| ! | ACTGGTTGTTTCACACGGGT+TGG | - | chr6.3:8630765-8630784 | MS.gene86087:intron | 50.0% |
| ! | ATGTGTGCGTTGGCACTTAC+TGG | - | chr6.3:8630747-8630766 | MS.gene86087:intron | 50.0% |
| !! | TATCGGTGTCGTGTAGGTGT+CGG | - | chr6.3:8630329-8630348 | MS.gene86087:intron | 50.0% |
| !! | TCGTGTAGGTGTCGGACACC+AGG | - | chr6.3:8630337-8630356 | MS.gene86087:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 8629565 | 8631020 | 8629565 | ID=MS.gene86087 |
| chr6.3 | mRNA | 8629565 | 8631020 | 8629565 | ID=MS.gene86087.t1;Parent=MS.gene86087 |
| chr6.3 | exon | 8630888 | 8631020 | 8630888 | ID=MS.gene86087.t1.exon1;Parent=MS.gene86087.t1 |
| chr6.3 | CDS | 8630888 | 8631020 | 8630888 | ID=cds.MS.gene86087.t1;Parent=MS.gene86087.t1 |
| chr6.3 | exon | 8629943 | 8630107 | 8629943 | ID=MS.gene86087.t1.exon2;Parent=MS.gene86087.t1 |
| chr6.3 | CDS | 8629943 | 8630107 | 8629943 | ID=cds.MS.gene86087.t1;Parent=MS.gene86087.t1 |
| chr6.3 | exon | 8629565 | 8629620 | 8629565 | ID=MS.gene86087.t1.exon3;Parent=MS.gene86087.t1 |
| chr6.3 | CDS | 8629565 | 8629620 | 8629565 | ID=cds.MS.gene86087.t1;Parent=MS.gene86087.t1 |
| Gene Sequence |
| Protein sequence |
