AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031394.t1 MTR_2g079700 94.005 417 23 1 1 415 1 417 0.0 793
MS.gene031394.t1 MTR_6g007490 65.707 417 141 2 1 415 25 441 0.0 589
MS.gene031394.t1 MTR_6g007490 62.541 307 113 2 1 305 25 331 1.40e-143 412
MS.gene031394.t1 MTR_3g086910 49.231 390 177 7 41 415 32 415 1.73e-118 351
MS.gene031394.t1 MTR_3g086910 46.953 279 128 6 41 305 32 304 1.55e-74 235
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031394.t1 AT1G52920 67.952 415 128 1 1 415 1 410 0.0 590
MS.gene031394.t1 AT2G20770 63.462 416 140 3 1 415 1 405 0.0 551
MS.gene031394.t1 AT5G65280 45.340 397 190 8 41 415 42 433 4.50e-113 338
MS.gene031394.t1 AT5G65280 47.573 309 147 5 121 415 4 311 6.73e-97 292

Find 115 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAGATCATCCACATTCT+TGG 0.288609 2.1:+20288155 None:intergenic
ATACCTCTTTGAATTGTCTA+AGG 0.293167 2.1:+20287244 None:intergenic
AGACCTTCGAATAAGGAATA+AGG 0.296203 2.1:+20286099 None:intergenic
CATCCACATTCTTGGTTACT+TGG 0.297066 2.1:+20288163 None:intergenic
ATGGCAGAACAGGTTACTTA+TGG 0.303883 2.1:-20287078 MS.gene031394:CDS
TCCTCCAAGACCTTCGAATA+AGG 0.318685 2.1:+20286092 None:intergenic
GGTGCTGCTCATGGACTTGC+AGG 0.320322 2.1:-20286799 MS.gene031394:CDS
CTCCTCCATGCATCTTTCCT+TGG 0.350097 2.1:+20286127 None:intergenic
CGTGCCGGTGTTTGTGCTCT+TGG 0.351585 2.1:-20287315 MS.gene031394:CDS
TTGACATCTTCCACCTCATC+TGG 0.364253 2.1:+20286742 None:intergenic
TATTCCTTATTCGAAGGTCT+TGG 0.377583 2.1:-20286096 MS.gene031394:CDS
CATTGGTGTCACGGTGCTCC+TGG 0.378975 2.1:-20286634 MS.gene031394:CDS
AATGGCATGGGAAGAAATAC+TGG 0.383288 2.1:-20286822 MS.gene031394:CDS
GAGTTATTGTATGGCAGAAC+AGG 0.390773 2.1:-20287088 MS.gene031394:CDS
CCACAAGTGGCTAGGTTCCC+TGG 0.391353 2.1:-20286039 MS.gene031394:CDS
GGTTCTTGGAACGGCATACT+TGG 0.395964 2.1:-20288199 MS.gene031394:CDS
TGTTCTTCAAGTGTTGTCTC+TGG 0.397728 2.1:+20288790 None:intergenic
AGCACTTCACTTCAAACAAT+TGG 0.406303 2.1:-20288668 MS.gene031394:intron
CAGGAGAAGTAGTGTGGAAG+AGG 0.407282 2.1:-20286302 MS.gene031394:CDS
GTTATAACTTCATCCACAAT+TGG 0.424250 2.1:+20286892 None:intergenic
GTCAGATTCACTTCCTTCAC+TGG 0.425272 2.1:+20286666 None:intergenic
CTTTCTCTGTACAGATTGAC+AGG 0.431846 2.1:-20286222 MS.gene031394:CDS
TGGCAGAACAGGTTACTTAT+GGG 0.432303 2.1:-20287077 MS.gene031394:CDS
TCATTCTTAAACAAGCATAT+CGG 0.434369 2.1:-20287049 MS.gene031394:CDS
TATACCTTTCTAGACATGAT+TGG 0.445743 2.1:-20286063 MS.gene031394:CDS
ATTCTGCTTCAGCAAATTCA+AGG 0.460631 2.1:-20288104 MS.gene031394:intron
GACCTTCGAATAAGGAATAA+GGG 0.464630 2.1:+20286100 None:intergenic
TCTGCCATACAATAACTCAT+AGG 0.466009 2.1:+20287093 None:intergenic
GTGTTTGGTAAGGTAGAGAA+AGG 0.471162 2.1:+20288713 None:intergenic
TCCTTATTCGAAGGTCTTGG+AGG 0.474374 2.1:-20286093 MS.gene031394:CDS
AAATACTGGGGTGCTGCTCA+TGG 0.483810 2.1:-20286808 MS.gene031394:CDS
CGCCCTTATTCCTTATTCGA+AGG 0.485112 2.1:-20286102 MS.gene031394:CDS
TTTATGCTTGAAGATTGTTA+AGG 0.488334 2.1:-20288133 MS.gene031394:CDS
GAGGTGAGAGAGTGTGGTGT+TGG 0.490011 2.1:+20288739 None:intergenic
ATGCCTTACCGTGTAGGTAG+TGG 0.491158 2.1:+20287012 None:intergenic
AGGGGTCTTCTTAAGCGAGT+TGG 0.504459 2.1:-20286282 MS.gene031394:CDS
GAGTACTTGTACAGGGCTAA+AGG 0.505457 2.1:-20286192 MS.gene031394:CDS
TCGCAAGCAGCGGAAGATGC+AGG 0.507394 2.1:-20286321 MS.gene031394:CDS
AGTGTTGTCTCTGGCATGAA+TGG 0.507407 2.1:+20288799 None:intergenic
TTGCCCTATGAGTTATTGTA+TGG 0.507703 2.1:-20287097 MS.gene031394:CDS
AGGAGTGAGGTGAGAGAGTG+TGG 0.508542 2.1:+20288733 None:intergenic
GTGACTCAAAGTTCATAGCC+AGG 0.511069 2.1:+20286021 None:intergenic
TTTGAGGTGAGTGTTTGGTA+AGG 0.514421 2.1:+20288703 None:intergenic
CAAGAGCACAAACACCGGCA+CGG 0.514774 2.1:+20287316 None:intergenic
GAAACAAGGAGTACTTGTAC+AGG 0.518932 2.1:-20286200 MS.gene031394:CDS
ACGTTCATCTGTGGCCGTGC+CGG 0.520688 2.1:-20287330 MS.gene031394:CDS
CGTCTTGTGCATTGGTGTCA+CGG 0.521333 2.1:-20286643 MS.gene031394:CDS
TGGACCAATCATGTCTAGAA+AGG 0.522222 2.1:+20286059 None:intergenic
ATGAATGGTGGCAAGTTGTT+CGG 0.523714 2.1:+20288814 None:intergenic
GTGGATGAAGTTATAACAGC+TGG 0.524603 2.1:-20286886 MS.gene031394:CDS
TGGTGTTGGTGTTTGAGAAG+AGG 0.528091 2.1:+20288753 None:intergenic
CTGCCATACAATAACTCATA+GGG 0.530788 2.1:+20287094 None:intergenic
ATTCGAAGGTCTTGGAGGAA+TGG 0.532742 2.1:-20286088 MS.gene031394:CDS
GGAATTTGCCACGGCATCAG+TGG 0.534765 2.1:-20286261 MS.gene031394:CDS
GGCAATTATCCCTCCAGTGA+AGG 0.536493 2.1:-20286679 MS.gene031394:CDS
GATGTCCTTTGATGTATGAA+TGG 0.539425 2.1:-20286840 MS.gene031394:CDS
AGCACCAAGAGCACAAACAC+CGG 0.540687 2.1:+20287311 None:intergenic
AGATGAGGTGGAAGATGTCA+AGG 0.556890 2.1:-20286740 MS.gene031394:CDS
AGTGAGACATGGGGTTCTAG+TGG 0.556968 2.1:-20288255 MS.gene031394:CDS
TTAAGGTGGTGAGTGAGACA+TGG 0.563607 2.1:-20288266 MS.gene031394:intron
TAAGGTGGTGAGTGAGACAT+GGG 0.563863 2.1:-20288265 MS.gene031394:intron
GCGCAAGAATTGATCGGCCA+AGG 0.565355 2.1:-20286144 MS.gene031394:CDS
AACTCATAGGGCAAATCAGT+GGG 0.573329 2.1:+20287106 None:intergenic
TGGTAGAAAATTAGCTCACA+AGG 0.574909 2.1:-20286866 MS.gene031394:CDS
GAAGGACTATACGCTTTACA+CGG 0.574930 2.1:-20288223 MS.gene031394:CDS
AGGAGAAGTAGTGTGGAAGA+GGG 0.575038 2.1:-20286301 MS.gene031394:CDS
GAATTTGCCACGGCATCAGT+GGG 0.580597 2.1:-20286260 MS.gene031394:CDS
TGCCTTACCGTGTAGGTAGT+GGG 0.583545 2.1:+20287013 None:intergenic
GTACAGAGAAAGAAAGACAT+AGG 0.584385 2.1:+20286233 None:intergenic
ATCGGCCAAGGAAAGATGCA+TGG 0.587677 2.1:-20286132 MS.gene031394:CDS
AGATTCACTTCCTTCACTGG+AGG 0.588226 2.1:+20286669 None:intergenic
GCTGTTATAGCTAAGCATGC+TGG 0.588262 2.1:-20287291 MS.gene031394:CDS
TGATTGGTCCACAAGTGGCT+AGG 0.590458 2.1:-20286047 MS.gene031394:CDS
CCAGGGAACCTAGCCACTTG+TGG 0.590886 2.1:+20286039 None:intergenic
CACAATACCCACTACCTACA+CGG 0.590900 2.1:-20287020 MS.gene031394:intron
GGAACTTAAGCCAGATGAGG+TGG 0.592388 2.1:-20286752 MS.gene031394:CDS
GATAGAGCGCAAGAATTGAT+CGG 0.594878 2.1:-20286150 MS.gene031394:CDS
GTAAGGTAGAGAAAGGAGTG+AGG 0.595969 2.1:+20288720 None:intergenic
GTACAGATTGACAGGAAACA+AGG 0.596022 2.1:-20286214 MS.gene031394:CDS
ACAAGCGTAAGAGTTACTCC+AGG 0.600044 2.1:+20286616 None:intergenic
ATGGCATGGGAAGAAATACT+GGG 0.602526 2.1:-20286821 MS.gene031394:CDS
CCATGCCATTCATACATCAA+AGG 0.604422 2.1:+20286835 None:intergenic
CTACCAAGTAACCAAGAATG+TGG 0.605333 2.1:-20288166 MS.gene031394:CDS
AATCTGACCGTCTTGTGCAT+TGG 0.606132 2.1:-20286651 MS.gene031394:CDS
AAGGACTATACGCTTTACAC+GGG 0.607848 2.1:-20288222 MS.gene031394:CDS
AAGCGAGTTGGAATTTGCCA+CGG 0.608188 2.1:-20286270 MS.gene031394:CDS
GAACATATGCCTTACCGTGT+AGG 0.609489 2.1:+20287006 None:intergenic
GATTCACTTCCTTCACTGGA+GGG 0.611653 2.1:+20286670 None:intergenic
AGGTGTTCCCACTGATGCCG+TGG 0.612064 2.1:+20286253 None:intergenic
TTTAGCCGGAGATGTCTGAT+CGG 0.613413 2.1:-20288843 None:intergenic
GGAGAAGTAGTGTGGAAGAG+GGG 0.618091 2.1:-20286300 MS.gene031394:CDS
TTCTAGTGGGAACCGTGTGA+AGG 0.618170 2.1:-20288241 MS.gene031394:CDS
GTGAGACATGGGGTTCTAGT+GGG 0.619681 2.1:-20288254 MS.gene031394:CDS
TTACCTTAGACAATTCAAAG+AGG 0.627912 2.1:-20287247 MS.gene031394:intron
TAACTCATAGGGCAAATCAG+TGG 0.629793 2.1:+20287105 None:intergenic
CTTTGATGTATGAATGGCAT+GGG 0.630619 2.1:-20286834 MS.gene031394:CDS
TGACTCAAAGTTCATAGCCA+GGG 0.635792 2.1:+20286022 None:intergenic
AGGACTATACGCTTTACACG+GGG 0.636276 2.1:-20288221 MS.gene031394:CDS
AAGATGCAGGAGAAGTAGTG+TGG 0.637341 2.1:-20286308 MS.gene031394:CDS
AGACATGATTGGTCCACAAG+TGG 0.638089 2.1:-20286052 MS.gene031394:CDS
GGCCACAGATGAACGTCACA+CGG 0.640066 2.1:+20287337 None:intergenic
TACGCTTGTAAAAGCAGCCG+AGG 0.641247 2.1:-20286602 MS.gene031394:intron
CGTGACACCAATGCACAAGA+CGG 0.665767 2.1:+20286644 None:intergenic
CATGGAACTTAAGCCAGATG+AGG 0.673066 2.1:-20286755 MS.gene031394:CDS
GTTGTCTCTGGCATGAATGG+TGG 0.674768 2.1:+20288802 None:intergenic
AGCCGTGTGACGTTCATCTG+TGG 0.685914 2.1:-20287339 MS.gene031394:CDS
CCTTTGATGTATGAATGGCA+TGG 0.688871 2.1:-20286835 MS.gene031394:CDS
GATGAGGTGGAAGATGTCAA+GGG 0.689281 2.1:-20286739 MS.gene031394:CDS
AAACAAGGAGTACTTGTACA+GGG 0.691885 2.1:-20286199 MS.gene031394:CDS
GGTAGAAAATTAGCTCACAA+GGG 0.710711 2.1:-20286865 MS.gene031394:CDS
GGACTATACGCTTTACACGG+GGG 0.714635 2.1:-20288220 MS.gene031394:CDS
AAGGTGGTGAGTGAGACATG+GGG 0.727439 2.1:-20288264 MS.gene031394:CDS
GGCCAAGGAAAGATGCATGG+AGG 0.732329 2.1:-20286129 MS.gene031394:CDS
AAAGCGTATAGTCCTTCACA+CGG 0.761639 2.1:+20288229 None:intergenic
TGGCATGGGAAGAAATACTG+GGG 0.762911 2.1:-20286820 MS.gene031394:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCATTATAAAAAAAAAAAA+GGG + chr2.1:20288290-20288309 None:intergenic 10.0%
!! AGAAAAAATGAAAATTATTG+TGG + chr2.1:20286220-20286239 None:intergenic 15.0%
!! CCTCATTATAAAAAAAAAAA+AGG + chr2.1:20288291-20288310 None:intergenic 15.0%
!!! AATTTTCATTTTTTCTTTGT+GGG - chr2.1:20286224-20286243 MS.gene031394:CDS 15.0%
!!! CCTTTTTTTTTTTTATAATG+AGG - chr2.1:20288288-20288307 MS.gene031394:intron 15.0%
!!! TAATTTTCATTTTTTCTTTG+TGG - chr2.1:20286223-20286242 MS.gene031394:CDS 15.0%
!!! TTGTTTATGTATTACTTTTA+AGG - chr2.1:20286575-20286594 MS.gene031394:intron 15.0%
!! AAACTTTGATTGTTCAATTT+AGG + chr2.1:20287434-20287453 None:intergenic 20.0%
!! CTAATTTCTTTACCATAATT+AGG - chr2.1:20288494-20288513 MS.gene031394:intron 20.0%
!! TATTAATTACGTTAAACAGA+TGG + chr2.1:20287112-20287131 None:intergenic 20.0%
!! TTCATCATAAACCATAAATT+TGG + chr2.1:20287064-20287083 None:intergenic 20.0%
!!! AAATTAAAAGCCAATTTTCA+TGG + chr2.1:20287931-20287950 None:intergenic 20.0%
!!! TCAATTTCTGTTTTTCATTT+TGG - chr2.1:20286253-20286272 MS.gene031394:CDS 20.0%
!!! TGTTAATATATTTTGTTGTC+TGG - chr2.1:20287316-20287335 MS.gene031394:CDS 20.0%
!!! TTATGATGAATTTTGAAACA+TGG - chr2.1:20287074-20287093 MS.gene031394:CDS 20.0%
!!! TTTACCATAATTAGGTTTTT+GGG - chr2.1:20288502-20288521 MS.gene031394:intron 20.0%
!!! TTTATGTATTACTTTTAAGG+TGG - chr2.1:20286578-20286597 MS.gene031394:intron 20.0%
! AAAAAAGGGAAATATTACCT+CGG + chr2.1:20288276-20288295 None:intergenic 25.0%
! AATGCAATTTAGATGGTTTA+TGG - chr2.1:20286899-20286918 MS.gene031394:intron 25.0%
! AGTTGAACATCTGAAAATAA+TGG + chr2.1:20287000-20287019 None:intergenic 25.0%
! GATTTCTGTACAATTTAATG+TGG - chr2.1:20286846-20286865 MS.gene031394:CDS 25.0%
! TAATGAAGTTACATTGGTAT+TGG + chr2.1:20286954-20286973 None:intergenic 25.0%
! TCACATAAATGCTTATGTAT+AGG + chr2.1:20286524-20286543 None:intergenic 25.0%
! TCATTCTTAAACAAGCATAT+CGG - chr2.1:20287809-20287828 MS.gene031394:intron 25.0%
! TCCAATTTACACATGAATTT+CGG - chr2.1:20287675-20287694 MS.gene031394:intron 25.0%
! TGCAAAAATGCAATTCAATT+AGG + chr2.1:20286376-20286395 None:intergenic 25.0%
! TTTATCCTTAGGATTAAGTT+AGG - chr2.1:20287366-20287385 MS.gene031394:intron 25.0%
! TTTATGCTTGAAGATTGTTA+AGG - chr2.1:20286725-20286744 MS.gene031394:CDS 25.0%
!! CTTTACCATAATTAGGTTTT+TGG - chr2.1:20288501-20288520 MS.gene031394:intron 25.0%
!! TGTGTTTTCTAATTCTTCTT+AGG - chr2.1:20286350-20286369 MS.gene031394:intron 25.0%
!! TTACCATAATTAGGTTTTTG+GGG - chr2.1:20288503-20288522 MS.gene031394:intron 25.0%
!!! GTTAAACTTCTTTTTCGTAT+TGG - chr2.1:20287700-20287719 MS.gene031394:intron 25.0%
!!! TTCTGTTTTTCATTTTGGTT+AGG - chr2.1:20286258-20286277 MS.gene031394:CDS 25.0%
AGATGGTTTATGGATATATC+AGG - chr2.1:20286909-20286928 MS.gene031394:intron 30.0%
AGTGGTTAATGCAATTTAGA+TGG - chr2.1:20286892-20286911 MS.gene031394:CDS 30.0%
ATACCTCTTTGAATTGTCTA+AGG + chr2.1:20287617-20287636 None:intergenic 30.0%
ATTAAAAATCGTTTCTCGAG+TGG - chr2.1:20288158-20288177 MS.gene031394:CDS 30.0%
ATTAACCACAAATTTGAACG+TGG - chr2.1:20286970-20286989 MS.gene031394:intron 30.0%
ATTTGAACACCTGGAAATTA+TGG - chr2.1:20287028-20287047 MS.gene031394:CDS 30.0%
CATATTCAAATACAAGCTTC+AGG - chr2.1:20288330-20288349 MS.gene031394:intron 30.0%
CATTTCCTAACTTAATCCTA+AGG + chr2.1:20287374-20287393 None:intergenic 30.0%
CCAATTTACACATGAATTTC+GGG - chr2.1:20287676-20287695 MS.gene031394:intron 30.0%
CTGAAGCTTGTATTTGAATA+TGG + chr2.1:20288332-20288351 None:intergenic 30.0%
CTTAGGATTAAGTTAGGAAA+TGG - chr2.1:20287372-20287391 MS.gene031394:intron 30.0%
GTCAATGACATCCAAATTTA+TGG - chr2.1:20287050-20287069 MS.gene031394:CDS 30.0%
GTTATAACTTCATCCACAAT+TGG + chr2.1:20287969-20287988 None:intergenic 30.0%
TACGTTAAACAGATGGTTTA+TGG + chr2.1:20287105-20287124 None:intergenic 30.0%
TATACCTTTCTAGACATGAT+TGG - chr2.1:20288795-20288814 MS.gene031394:CDS 30.0%
TATCCCCAAAAACCTAATTA+TGG + chr2.1:20288509-20288528 None:intergenic 30.0%
TCAGGTTTGTGCAAATTTAT+CGG + chr2.1:20288380-20288399 None:intergenic 30.0%
TGTGGTTAATGAAGTTACAT+TGG + chr2.1:20286960-20286979 None:intergenic 30.0%
TTACCTTAGACAATTCAAAG+AGG - chr2.1:20287611-20287630 MS.gene031394:intron 30.0%
! AGGAATTATGAACGTTTTGA+TGG - chr2.1:20288079-20288098 MS.gene031394:intron 30.0%
! ATTTTGCAGTGTTCATTGTA+TGG - chr2.1:20288409-20288428 MS.gene031394:intron 30.0%
! TCCTTTACATCATATTGACT+CGG + chr2.1:20288356-20288375 None:intergenic 30.0%
! TTTAAAACTACGCCTTACAA+CGG + chr2.1:20286818-20286837 None:intergenic 30.0%
! TTTTTGACAGAAACCAATTG+TGG - chr2.1:20287953-20287972 MS.gene031394:intron 30.0%
!! ATAATTAGGTTTTTGGGGAT+AGG - chr2.1:20288508-20288527 MS.gene031394:intron 30.0%
!! TAATTAGGTTTTTGGGGATA+GGG - chr2.1:20288509-20288528 MS.gene031394:intron 30.0%
!!! CGTATTGGTGCTTTTATTTT+TGG - chr2.1:20287715-20287734 MS.gene031394:intron 30.0%
AAGGCATATGTTCTTCATCA+TGG - chr2.1:20287862-20287881 MS.gene031394:intron 35.0%
ACTAAGATCATCCACATTCT+TGG + chr2.1:20286706-20286725 None:intergenic 35.0%
AGACCTTCGAATAAGGAATA+AGG + chr2.1:20288762-20288781 None:intergenic 35.0%
AGCACTTCACTTCAAACAAT+TGG - chr2.1:20286190-20286209 MS.gene031394:CDS 35.0%
ATCAAAGTTTCAGCCTGAAA+TGG - chr2.1:20287444-20287463 MS.gene031394:intron 35.0%
ATTCTGCTTCAGCAAATTCA+AGG - chr2.1:20286754-20286773 MS.gene031394:CDS 35.0%
ATTTGCTCGTGTTTATCCTT+AGG - chr2.1:20287355-20287374 MS.gene031394:intron 35.0%
CAACGAAATTTAGACAGCTT+TGG + chr2.1:20287173-20287192 None:intergenic 35.0%
CAAGTTAGAAACTTTCACGT+TGG - chr2.1:20286283-20286302 MS.gene031394:CDS 35.0%
CCCGAAATTCATGTGTAAAT+TGG + chr2.1:20287679-20287698 None:intergenic 35.0%
CTGCCATACAATAACTCATA+GGG + chr2.1:20287767-20287786 None:intergenic 35.0%
CTTTGATGTATGAATGGCAT+GGG - chr2.1:20288024-20288043 MS.gene031394:intron 35.0%
GACCTTCGAATAAGGAATAA+GGG + chr2.1:20288761-20288780 None:intergenic 35.0%
GATGTCCTTTGATGTATGAA+TGG - chr2.1:20288018-20288037 MS.gene031394:intron 35.0%
GGTAGAAAATTAGCTCACAA+GGG - chr2.1:20287993-20288012 MS.gene031394:intron 35.0%
GTACAGAGAAAGAAAGACAT+AGG + chr2.1:20288628-20288647 None:intergenic 35.0%
GTTAACCACGTTCAAATTTG+TGG + chr2.1:20286978-20286997 None:intergenic 35.0%
TACTCTCTATTTCATGCATC+TGG - chr2.1:20286777-20286796 MS.gene031394:CDS 35.0%
TATTCCTTATTCGAAGGTCT+TGG - chr2.1:20288762-20288781 MS.gene031394:CDS 35.0%
TCAAAGTTTCAGCCTGAAAT+GGG - chr2.1:20287445-20287464 MS.gene031394:intron 35.0%
TCTGCCATACAATAACTCAT+AGG + chr2.1:20287768-20287787 None:intergenic 35.0%
TGGTAGAAAATTAGCTCACA+AGG - chr2.1:20287992-20288011 MS.gene031394:intron 35.0%
TGTCATTGACCATAATTTCC+AGG + chr2.1:20287040-20287059 None:intergenic 35.0%
TTGCCCTATGAGTTATTGTA+TGG - chr2.1:20287761-20287780 MS.gene031394:intron 35.0%
TTGGCTACAACTTCTATAAG+TGG + chr2.1:20287154-20287173 None:intergenic 35.0%
TTTAATGTGGACAACTTTCG+CGG - chr2.1:20286859-20286878 MS.gene031394:CDS 35.0%
TTTCACGTTGGTGCAATAAT+GGG - chr2.1:20286295-20286314 MS.gene031394:CDS 35.0%
! TGAAGTTACATTGGTATTGG+TGG + chr2.1:20286951-20286970 None:intergenic 35.0%
!! AAACAAGGAGTACTTGTACA+GGG - chr2.1:20288659-20288678 MS.gene031394:intron 35.0%
!! AACGGCATACTTGGTTTTTA+AGG - chr2.1:20286668-20286687 MS.gene031394:CDS 35.0%
!! TATGAACGTTTTGATGGACA+TGG - chr2.1:20288085-20288104 MS.gene031394:intron 35.0%
!! TGCTGTAGTTTGTAGTTTTG+AGG + chr2.1:20286174-20286193 None:intergenic 35.0%
!! TTTCGCGGTAACTTTTTAAG+TGG - chr2.1:20286874-20286893 MS.gene031394:CDS 35.0%
AAAGCGTATAGTCCTTCACA+CGG + chr2.1:20286632-20286651 None:intergenic 40.0%
AACTCATAGGGCAAATCAGT+GGG + chr2.1:20287755-20287774 None:intergenic 40.0%
AAGGACTATACGCTTTACAC+GGG - chr2.1:20286636-20286655 MS.gene031394:CDS 40.0%
AATGGCATGGGAAGAAATAC+TGG - chr2.1:20288036-20288055 MS.gene031394:intron 40.0%
ATGAATGGTGGCAAGTTGTT+CGG + chr2.1:20286047-20286066 None:intergenic 40.0%
ATGGCAGAACAGGTTACTTA+TGG - chr2.1:20287780-20287799 MS.gene031394:intron 40.0%
ATGGCATGGGAAGAAATACT+GGG - chr2.1:20288037-20288056 MS.gene031394:intron 40.0%
CATCCACATTCTTGGTTACT+TGG + chr2.1:20286698-20286717 None:intergenic 40.0%
CCATGCCATTCATACATCAA+AGG + chr2.1:20288026-20288045 None:intergenic 40.0%
CCTTTGATGTATGAATGGCA+TGG - chr2.1:20288023-20288042 MS.gene031394:intron 40.0%
CTACCAAGTAACCAAGAATG+TGG - chr2.1:20286692-20286711 MS.gene031394:CDS 40.0%
CTGCAAAATCCATTGCAATC+AGG + chr2.1:20288398-20288417 None:intergenic 40.0%
CTTTCACGTTGGTGCAATAA+TGG - chr2.1:20286294-20286313 MS.gene031394:CDS 40.0%
CTTTCTCTGTACAGATTGAC+AGG - chr2.1:20288636-20288655 MS.gene031394:intron 40.0%
GAAGGACTATACGCTTTACA+CGG - chr2.1:20286635-20286654 MS.gene031394:CDS 40.0%
GACTTAACTTAGCCGTTGTA+AGG - chr2.1:20286803-20286822 MS.gene031394:CDS 40.0%
GAGTTATTGTATGGCAGAAC+AGG - chr2.1:20287770-20287789 MS.gene031394:intron 40.0%
GATAGAGCGCAAGAATTGAT+CGG - chr2.1:20288708-20288727 MS.gene031394:CDS 40.0%
GCCGAGTCAATATGATGTAA+AGG - chr2.1:20288352-20288371 MS.gene031394:intron 40.0%
GTACAGATTGACAGGAAACA+AGG - chr2.1:20288644-20288663 MS.gene031394:intron 40.0%
GTGGATGAAGTTATAACAGC+TGG - chr2.1:20287972-20287991 MS.gene031394:intron 40.0%
GTGGTTAATGCACTTCGAAT+AGG + chr2.1:20287135-20287154 None:intergenic 40.0%
GTGTTTGGTAAGGTAGAGAA+AGG + chr2.1:20286148-20286167 None:intergenic 40.0%
TAACTCATAGGGCAAATCAG+TGG + chr2.1:20287756-20287775 None:intergenic 40.0%
TCCAGGTGTTCAAATCTACA+TGG + chr2.1:20287023-20287042 None:intergenic 40.0%
TCCATGTAGATTTGAACACC+TGG - chr2.1:20287019-20287038 MS.gene031394:intron 40.0%
TGGACCAATCATGTCTAGAA+AGG + chr2.1:20288802-20288821 None:intergenic 40.0%
TGGCAGAACAGGTTACTTAT+GGG - chr2.1:20287781-20287800 MS.gene031394:intron 40.0%
TTGCACAAACCTGATTGCAA+TGG - chr2.1:20288386-20288405 MS.gene031394:intron 40.0%
! TGTTCTTCAAGTGTTGTCTC+TGG + chr2.1:20286071-20286090 None:intergenic 40.0%
! TTTGAGGTGAGTGTTTGGTA+AGG + chr2.1:20286158-20286177 None:intergenic 40.0%
!! GAAACAAGGAGTACTTGTAC+AGG - chr2.1:20288658-20288677 MS.gene031394:intron 40.0%
!! TGTCGACTTGCCATGAAAAT+TGG - chr2.1:20287918-20287937 MS.gene031394:intron 40.0%
!!! GTAGTTTTGAGGTGAGTGTT+TGG + chr2.1:20286163-20286182 None:intergenic 40.0%
AAATGGGAGGATGGGAAGTA+AGG - chr2.1:20287461-20287480 MS.gene031394:intron 45.0%
AAGATGCAGGAGAAGTAGTG+TGG - chr2.1:20288550-20288569 MS.gene031394:intron 45.0%
AAGTTTCAGCCTGAAATGGG+AGG - chr2.1:20287448-20287467 MS.gene031394:intron 45.0%
AATCTGACCGTCTTGTGCAT+TGG - chr2.1:20288207-20288226 MS.gene031394:CDS 45.0%
ACAAGCGTAAGAGTTACTCC+AGG + chr2.1:20288245-20288264 None:intergenic 45.0%
AGATGAGGTGGAAGATGTCA+AGG - chr2.1:20288118-20288137 MS.gene031394:CDS 45.0%
AGATTCACTTCCTTCACTGG+AGG + chr2.1:20288192-20288211 None:intergenic 45.0%
AGGACTATACGCTTTACACG+GGG - chr2.1:20286637-20286656 MS.gene031394:CDS 45.0%
AGGAGAAGTAGTGTGGAAGA+GGG - chr2.1:20288557-20288576 MS.gene031394:intron 45.0%
AGTGTTGTCTCTGGCATGAA+TGG + chr2.1:20286062-20286081 None:intergenic 45.0%
ATTCGAAGGTCTTGGAGGAA+TGG - chr2.1:20288770-20288789 MS.gene031394:CDS 45.0%
CACAATACCCACTACCTACA+CGG - chr2.1:20287838-20287857 MS.gene031394:intron 45.0%
CATGGAACTTAAGCCAGATG+AGG - chr2.1:20288103-20288122 MS.gene031394:intron 45.0%
CGCCCTTATTCCTTATTCGA+AGG - chr2.1:20288756-20288775 MS.gene031394:CDS 45.0%
GAACATATGCCTTACCGTGT+AGG + chr2.1:20287855-20287874 None:intergenic 45.0%
GAGTACTTGTACAGGGCTAA+AGG - chr2.1:20288666-20288685 MS.gene031394:intron 45.0%
GATGAGGTGGAAGATGTCAA+GGG - chr2.1:20288119-20288138 MS.gene031394:CDS 45.0%
GATTCACTTCCTTCACTGGA+GGG + chr2.1:20288191-20288210 None:intergenic 45.0%
GCTGTTATAGCTAAGCATGC+TGG - chr2.1:20287567-20287586 MS.gene031394:intron 45.0%
GTAAGGTAGAGAAAGGAGTG+AGG + chr2.1:20286141-20286160 None:intergenic 45.0%
GTCAGATTCACTTCCTTCAC+TGG + chr2.1:20288195-20288214 None:intergenic 45.0%
TAAGGTGGTGAGTGAGACAT+GGG - chr2.1:20286593-20286612 MS.gene031394:intron 45.0%
TCCTCCAAGACCTTCGAATA+AGG + chr2.1:20288769-20288788 None:intergenic 45.0%
TCCTTATTCGAAGGTCTTGG+AGG - chr2.1:20288765-20288784 MS.gene031394:CDS 45.0%
TGGCATGGGAAGAAATACTG+GGG - chr2.1:20288038-20288057 MS.gene031394:intron 45.0%
TTAAGGTGGTGAGTGAGACA+TGG - chr2.1:20286592-20286611 MS.gene031394:intron 45.0%
TTGACATCTTCCACCTCATC+TGG + chr2.1:20288119-20288138 None:intergenic 45.0%
! AAGCGAGTTGGAATTTGCCA+CGG - chr2.1:20288588-20288607 MS.gene031394:intron 45.0%
! AGACATGATTGGTCCACAAG+TGG - chr2.1:20288806-20288825 MS.gene031394:CDS 45.0%
! TAGGGAATTTTCGCAAGCAG+CGG - chr2.1:20288527-20288546 MS.gene031394:intron 45.0%
!! ACGTTGGTGCAATAATGGGT+CGG - chr2.1:20286299-20286318 MS.gene031394:CDS 45.0%
!! TGGTGTTGGTGTTTGAGAAG+AGG + chr2.1:20286108-20286127 None:intergenic 45.0%
AAGGTGGTGAGTGAGACATG+GGG - chr2.1:20286594-20286613 MS.gene031394:intron 50.0%
ACTTCCCATCCTCCCATTTC+AGG + chr2.1:20287460-20287479 None:intergenic 50.0%
AGGGGTCTTCTTAAGCGAGT+TGG - chr2.1:20288576-20288595 MS.gene031394:intron 50.0%
ATCGGCCAAGGAAAGATGCA+TGG - chr2.1:20288726-20288745 MS.gene031394:CDS 50.0%
ATGCCTTACCGTGTAGGTAG+TGG + chr2.1:20287849-20287868 None:intergenic 50.0%
CAGGAGAAGTAGTGTGGAAG+AGG - chr2.1:20288556-20288575 MS.gene031394:intron 50.0%
CGTGACACCAATGCACAAGA+CGG + chr2.1:20288217-20288236 None:intergenic 50.0%
CTCCTCCATGCATCTTTCCT+TGG + chr2.1:20288734-20288753 None:intergenic 50.0%
GAATTTGCCACGGCATCAGT+GGG - chr2.1:20288598-20288617 MS.gene031394:intron 50.0%
GGAACTTAAGCCAGATGAGG+TGG - chr2.1:20288106-20288125 MS.gene031394:CDS 50.0%
GGACTATACGCTTTACACGG+GGG - chr2.1:20286638-20286657 MS.gene031394:CDS 50.0%
GGAGAAGTAGTGTGGAAGAG+GGG - chr2.1:20288558-20288577 MS.gene031394:intron 50.0%
GGCAATTATCCCTCCAGTGA+AGG - chr2.1:20288179-20288198 MS.gene031394:CDS 50.0%
GGTTCTTGGAACGGCATACT+TGG - chr2.1:20286659-20286678 MS.gene031394:CDS 50.0%
GTTGTCTCTGGCATGAATGG+TGG + chr2.1:20286059-20286078 None:intergenic 50.0%
TACCCACTACCTACACGGTA+AGG - chr2.1:20287843-20287862 MS.gene031394:intron 50.0%
TACGCTTGTAAAAGCAGCCG+AGG - chr2.1:20288256-20288275 MS.gene031394:CDS 50.0%
TCAGCCTGAAATGGGAGGAT+GGG - chr2.1:20287453-20287472 MS.gene031394:intron 50.0%
TGCCTTACCGTGTAGGTAGT+GGG + chr2.1:20287848-20287867 None:intergenic 50.0%
TTACACGGGGGTTCTTGGAA+CGG - chr2.1:20286650-20286669 MS.gene031394:CDS 50.0%
TTCAGCCTGAAATGGGAGGA+TGG - chr2.1:20287452-20287471 MS.gene031394:intron 50.0%
TTCTAGTGGGAACCGTGTGA+AGG - chr2.1:20286617-20286636 MS.gene031394:CDS 50.0%
! AGCACCAAGAGCACAAACAC+CGG + chr2.1:20287550-20287569 None:intergenic 50.0%
! AGTGAGACATGGGGTTCTAG+TGG - chr2.1:20286603-20286622 MS.gene031394:CDS 50.0%
! GTGAGACATGGGGTTCTAGT+GGG - chr2.1:20286604-20286623 MS.gene031394:CDS 50.0%
! TGATTGGTCCACAAGTGGCT+AGG - chr2.1:20288811-20288830 MS.gene031394:CDS 50.0%
!! AAATACTGGGGTGCTGCTCA+TGG - chr2.1:20288050-20288069 MS.gene031394:intron 50.0%
!! CGTCTTGTGCATTGGTGTCA+CGG - chr2.1:20288215-20288234 MS.gene031394:CDS 50.0%
ACGCTTTACACGGGGGTTCT+TGG - chr2.1:20286645-20286664 MS.gene031394:CDS 55.0%
AGCCGTGTGACGTTCATCTG+TGG - chr2.1:20287519-20287538 MS.gene031394:intron 55.0%
AGGAGTGAGGTGAGAGAGTG+TGG + chr2.1:20286128-20286147 None:intergenic 55.0%
GCGCAAGAATTGATCGGCCA+AGG - chr2.1:20288714-20288733 MS.gene031394:CDS 55.0%
GGAATTTGCCACGGCATCAG+TGG - chr2.1:20288597-20288616 MS.gene031394:intron 55.0%
GGCCAAGGAAAGATGCATGG+AGG - chr2.1:20288729-20288748 MS.gene031394:CDS 55.0%
GGCCACAGATGAACGTCACA+CGG + chr2.1:20287524-20287543 None:intergenic 55.0%
TGGGAGGATGGGAAGTAAGG+TGG - chr2.1:20287464-20287483 MS.gene031394:intron 55.0%
! CAAGAGCACAAACACCGGCA+CGG + chr2.1:20287545-20287564 None:intergenic 55.0%
! GAGGTGAGAGAGTGTGGTGT+TGG + chr2.1:20286122-20286141 None:intergenic 55.0%
ACGTTCATCTGTGGCCGTGC+CGG - chr2.1:20287528-20287547 MS.gene031394:intron 60.0%
AGGTGTTCCCACTGATGCCG+TGG + chr2.1:20288608-20288627 None:intergenic 60.0%
CCACAAGTGGCTAGGTTCCC+TGG - chr2.1:20288819-20288838 MS.gene031394:CDS 60.0%
CCAGGGAACCTAGCCACTTG+TGG + chr2.1:20288822-20288841 None:intergenic 60.0%
TCGCAAGCAGCGGAAGATGC+AGG - chr2.1:20288537-20288556 MS.gene031394:intron 60.0%
! CGTGCCGGTGTTTGTGCTCT+TGG - chr2.1:20287543-20287562 MS.gene031394:intron 60.0%
!! CATTGGTGTCACGGTGCTCC+TGG - chr2.1:20288224-20288243 MS.gene031394:CDS 60.0%
!! GGTGCTGCTCATGGACTTGC+AGG - chr2.1:20288059-20288078 MS.gene031394:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 20286026 20288854 20286026 ID=MS.gene031394
chr2.1 mRNA 20286026 20288854 20286026 ID=MS.gene031394.t1;Parent=MS.gene031394
chr2.1 exon 20288669 20288854 20288669 ID=MS.gene031394.t1.exon1;Parent=MS.gene031394.t1
chr2.1 CDS 20288669 20288854 20288669 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
chr2.1 exon 20288105 20288283 20288105 ID=MS.gene031394.t1.exon2;Parent=MS.gene031394.t1
chr2.1 CDS 20288105 20288283 20288105 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
chr2.1 exon 20287248 20287359 20287248 ID=MS.gene031394.t1.exon3;Parent=MS.gene031394.t1
chr2.1 CDS 20287248 20287359 20287248 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
chr2.1 exon 20287021 20287137 20287021 ID=MS.gene031394.t1.exon4;Parent=MS.gene031394.t1
chr2.1 CDS 20287021 20287137 20287021 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
chr2.1 exon 20286603 20286917 20286603 ID=MS.gene031394.t1.exon5;Parent=MS.gene031394.t1
chr2.1 CDS 20286603 20286917 20286603 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
chr2.1 exon 20286026 20286364 20286026 ID=MS.gene031394.t1.exon6;Parent=MS.gene031394.t1
chr2.1 CDS 20286026 20286364 20286026 ID=cds.MS.gene031394.t1;Parent=MS.gene031394.t1
Gene Sequence

>MS.gene031394

ATGTCTGATCGGTTTTTTCCGAACAACTTGCCACCATTCATGCCAGAGACAACACTTGAAGAACAAGAAGCAGAAGCAACCTCTTCTCAAACACCAACACCACACTCTCTCACCTCACTCCTTTCTCTACCTTACCAAACACTCACCTCAAAACTACAAACTACAGCACTTCACTTCAAACAATTGGTACTCCACAATAATTTTCATTTTTTCTTTGTGGGTTTCTTTCAATTTCTGTTTTTCATTTTGGTTAGGTTCAAGTTAGAAACTTTCACGTTGGTGCAATAATGGGTCGGTGTTTATTTGCTCACAAAGTGTTTGTGCTGTGTTTTCTAATTCTTCTTAGGCCTAATTGAATTGCATTTTTGCATTTATCTACTATTTGAATTGCTGTTTTTAGCTTATTTTCATACTCGCATGACTTATGAAAACAATTCATAACTTATATGATGTATAAATAGTTTGAATTTATTGTATCTTTTGTTAAAGAAATAACCTATACATAAGCATTTATGTGATAGACGCTTATCTTATAAGTATTTAACTAAGTTGTTTATGTATTACTTTTAAGGTGGTGAGTGAGACATGGGGTTCTAGTGGGAACCGTGTGAAGGACTATACGCTTTACACGGGGGTTCTTGGAACGGCATACTTGGTTTTTAAGGTCTACCAAGTAACCAAGAATGTGGATGATCTTAGTTTATGCTTGAAGATTGTTAAGGCTTGTGATTCTGCTTCAGCAAATTCAAGGTACTCTCTATTTCATGCATCTGGTAAGACTTAACTTAGCCGTTGTAAGGCGTAGTTTTAAAAGAGAAATGATTTCTGTACAATTTAATGTGGACAACTTTCGCGGTAACTTTTTAAGTGGTTAATGCAATTTAGATGGTTTATGGATATATCAGGTGTTGAAACTCATGTACCACCAATACCAATGTAACTTCATTAACCACAAATTTGAACGTGGTTAACCATTATTTTCAGATGTTCAACTCCATGTAGATTTGAACACCTGGAAATTATGGTCAATGACATCCAAATTTATGGTTTATGATGAATTTTGAAACATGGTATGTCCATAAACCATCTGTTTAACGTAATTAATACCTATTCGAAGTGCATTAACCACTTATAGAAGTTGTAGCCAAAGCTGTCTAAATTTCGTTGTTCAAAATGTGCAACTGTTTTAAAATGCTCAGTTAGAGAATTTTAATTGTTAAGAATAGCTTGCTATGAATAGTTAGATTTTGAAGTATAATGACAATAGAACTGCTTGTGTATTAGCTTATCTGTTAATATATTTTGTTGTCTGGTTATGTTACTTGAGAAATTTGCTCGTGTTTATCCTTAGGATTAAGTTAGGAAATGGCTTAAACTGTAGTTTCAAACTTGTGTTTTGAAATCACCTAAATTGAACAATCAAAGTTTCAGCCTGAAATGGGAGGATGGGAAGTAAGGTGGTTTTAGTTTTATTTGCTAATGTATTGAGTTGCAGCCGTGTGACGTTCATCTGTGGCCGTGCCGGTGTTTGTGCTCTTGGTGCTGTTATAGCTAAGCATGCTGGTGATGAGAGATTACTTGACTATTACCTTAGACAATTCAAAGAGGTATTTTGTTGTATTTATTTATGAATCACGATCTTATGATAGTCCAATTTACACATGAATTTCGGGTGTTAAACTTCTTTTTCGTATTGGTGCTTTTATTTTTGGAACAGATTGAGCTACCCACTGATTTGCCCTATGAGTTATTGTATGGCAGAACAGGTTACTTATGGGCTTGTTCATTCTTAAACAAGCATATCGGTAAAGACACAATACCCACTACCTACACGGTAAGGCATATGTTCTTCATCATGGCGTTCTTCTTTGCAATTTAACTTGTCAAAATCATGTCGACTTGCCATGAAAATTGGCTTTTAATTTAATTTTTGACAGAAACCAATTGTGGATGAAGTTATAACAGCTGGTAGAAAATTAGCTCACAAGGGAAGATGTCCTTTGATGTATGAATGGCATGGGAAGAAATACTGGGGTGCTGCTCATGGACTTGCAGGAATTATGAACGTTTTGATGGACATGGAACTTAAGCCAGATGAGGTGGAAGATGTCAAGGGTACGCTACGCTACATGATTAAAAATCGTTTCTCGAGTGGCAATTATCCCTCCAGTGAAGGAAGTGAATCTGACCGTCTTGTGCATTGGTGTCACGGTGCTCCTGGAGTAACTCTTACGCTTGTAAAAGCAGCCGAGGTAATATTTCCCTTTTTTTTTTTTATAATGAGGCAATTTTCTCATTCGTTACCATATTCAAATACAAGCTTCAGGCCGAGTCAATATGATGTAAAGGAACCGATAAATTTGCACAAACCTGATTGCAATGGATTTTGCAGTGTTCATTGTATGGTGTATTTAAACAGTTAAACTGAAAGTGATCTTGACAATTCAATGAAAATTAAGATCACATGACTAATTTCTTTACCATAATTAGGTTTTTGGGGATAGGGAATTTTCGCAAGCAGCGGAAGATGCAGGAGAAGTAGTGTGGAAGAGGGGTCTTCTTAAGCGAGTTGGAATTTGCCACGGCATCAGTGGGAACACCTATGTCTTTCTTTCTCTGTACAGATTGACAGGAAACAAGGAGTACTTGTACAGGGCTAAAGGATTTTCTTGCTTTCTACTTGATAGAGCGCAAGAATTGATCGGCCAAGGAAAGATGCATGGAGGAGATCGCCCTTATTCCTTATTCGAAGGTCTTGGAGGAATGGCATATACCTTTCTAGACATGATTGGTCCACAAGTGGCTAGGTTCCCTGGCTATGAACTTTGA
Protein sequence

>MS.gene031394.t1

MSDRFFPNNLPPFMPETTLEEQEAEATSSQTPTPHSLTSLLSLPYQTLTSKLQTTALHFKQLVVSETWGSSGNRVKDYTLYTGVLGTAYLVFKVYQVTKNVDDLSLCLKIVKACDSASANSSRVTFICGRAGVCALGAVIAKHAGDERLLDYYLRQFKEIELPTDLPYELLYGRTGYLWACSFLNKHIGKDTIPTTYTKPIVDEVITAGRKLAHKGRCPLMYEWHGKKYWGAAHGLAGIMNVLMDMELKPDEVEDVKGTLRYMIKNRFSSGNYPSSEGSESDRLVHWCHGAPGVTLTLVKAAEVFGDREFSQAAEDAGEVVWKRGLLKRVGICHGISGNTYVFLSLYRLTGNKEYLYRAKGFSCFLLDRAQELIGQGKMHGGDRPYSLFEGLGGMAYTFLDMIGPQVARFPGYEL