Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040398.t1 | XP_013448021.1 | 98.1 | 263 | 5 | 0 | 1 | 263 | 150 | 412 | 3.10E-146 | 527.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040398.t1 | Q67ZU1 | 63.0 | 262 | 97 | 0 | 1 | 262 | 155 | 416 | 3.2e-99 | 362.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040398.t1 | A0A072TYW1 | 98.1 | 263 | 5 | 0 | 1 | 263 | 150 | 412 | 2.2e-146 | 527.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene040398 | MS.gene029609 | PPI |
| MS.gene040398 | MS.gene71663 | PPI |
| MS.gene040398 | MS.gene060313 | PPI |
| MS.gene029607 | MS.gene040398 | PPI |
| MS.gene040398 | MS.gene060315 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040398.t1 | MTR_7g029365 | 98.099 | 263 | 5 | 0 | 1 | 263 | 150 | 412 | 0.0 | 538 |
| MS.gene040398.t1 | MTR_6g023740 | 59.696 | 263 | 104 | 2 | 2 | 262 | 151 | 413 | 9.31e-114 | 333 |
| MS.gene040398.t1 | MTR_7g029365 | 98.726 | 157 | 2 | 0 | 1 | 157 | 150 | 306 | 2.42e-111 | 323 |
| MS.gene040398.t1 | MTR_6g023750 | 56.061 | 264 | 114 | 2 | 1 | 262 | 93 | 356 | 2.26e-106 | 312 |
| MS.gene040398.t1 | MTR_5g084950 | 53.668 | 259 | 120 | 0 | 2 | 260 | 141 | 399 | 3.01e-101 | 301 |
| MS.gene040398.t1 | MTR_6g023715 | 53.612 | 263 | 101 | 5 | 2 | 262 | 151 | 394 | 5.13e-93 | 280 |
| MS.gene040398.t1 | MTR_7g081050 | 47.893 | 261 | 136 | 0 | 1 | 261 | 143 | 403 | 6.43e-92 | 277 |
| MS.gene040398.t1 | MTR_3g081060 | 47.909 | 263 | 135 | 2 | 1 | 261 | 147 | 409 | 8.39e-89 | 270 |
| MS.gene040398.t1 | MTR_3g081040 | 47.328 | 262 | 137 | 1 | 1 | 261 | 143 | 404 | 8.00e-87 | 264 |
| MS.gene040398.t1 | MTR_6g033965 | 45.420 | 262 | 140 | 2 | 1 | 261 | 143 | 402 | 1.57e-81 | 251 |
| MS.gene040398.t1 | MTR_3g080980 | 44.444 | 261 | 145 | 0 | 1 | 261 | 143 | 403 | 4.15e-76 | 237 |
| MS.gene040398.t1 | MTR_6g033975 | 43.893 | 262 | 143 | 3 | 1 | 261 | 143 | 401 | 6.02e-75 | 234 |
| MS.gene040398.t1 | MTR_7g081060 | 41.825 | 263 | 151 | 2 | 1 | 262 | 143 | 404 | 5.51e-72 | 226 |
| MS.gene040398.t1 | MTR_1g107330 | 38.783 | 263 | 155 | 3 | 2 | 264 | 144 | 400 | 6.32e-67 | 213 |
| MS.gene040398.t1 | MTR_1g107330 | 41.667 | 156 | 90 | 1 | 2 | 157 | 144 | 298 | 1.70e-37 | 135 |
| MS.gene040398.t1 | MTR_0595s0010 | 64.706 | 85 | 29 | 1 | 2 | 85 | 151 | 235 | 1.96e-33 | 124 |
| MS.gene040398.t1 | MTR_5g006650 | 57.143 | 84 | 31 | 1 | 2 | 85 | 125 | 203 | 4.75e-25 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040398.t1 | AT5G14180 | 62.977 | 262 | 97 | 0 | 1 | 262 | 55 | 316 | 2.88e-129 | 369 |
| MS.gene040398.t1 | AT5G14180 | 62.977 | 262 | 97 | 0 | 1 | 262 | 46 | 307 | 3.42e-129 | 369 |
| MS.gene040398.t1 | AT5G14180 | 62.977 | 262 | 97 | 0 | 1 | 262 | 155 | 416 | 4.03e-128 | 370 |
| MS.gene040398.t1 | AT5G14180 | 64.331 | 157 | 56 | 0 | 1 | 157 | 155 | 311 | 3.20e-74 | 229 |
| MS.gene040398.t1 | AT2G15230 | 36.154 | 260 | 160 | 3 | 2 | 261 | 132 | 385 | 3.72e-58 | 190 |
| MS.gene040398.t1 | AT5G14180 | 54.930 | 142 | 62 | 1 | 1 | 140 | 155 | 296 | 6.74e-53 | 174 |
Find 0 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATATATAAAATATTAAT+TGG | + | 51471:17923-17942 | MS.gene040398:intergenic | 0.0% |
| !! | TAATATATAAAATATTAATT+GGG | + | 51471:17922-17941 | MS.gene040398:intergenic | 0.0% |
| !! | TATAGTATAAATGTTTATAT+TGG | + | 51471:18082-18101 | MS.gene040398:intergenic | 10.0% |
| !!! | CATAATTTTATACATAAAAA+AGG | - | 51471:17758-17777 | MS.gene040398:intron | 10.0% |
| !! | ACAAATAGAATAGTAAAAAA+AGG | + | 51471:17431-17450 | MS.gene040398:intergenic | 15.0% |
| !! | AGAACATTATTAATTGTTTA+AGG | - | 51471:18405-18424 | MS.gene040398:intron | 15.0% |
| !! | CAAATAGAATAGTAAAAAAA+GGG | + | 51471:17430-17449 | MS.gene040398:intergenic | 15.0% |
| !! | CTAACATTTAATTAGATATT+TGG | - | 51471:18653-18672 | MS.gene040398:intron | 15.0% |
| !! | TCATGTAAAAAAGTAAGTAA+TGG | + | 51471:17331-17350 | MS.gene040398:intergenic | 20.0% |
| !!! | ACATTTTTTTTTTTGCATGA+TGG | + | 51471:18362-18381 | MS.gene040398:intergenic | 20.0% |
| !!! | TTGAGTTTTTTTTAAATACG+AGG | - | 51471:18322-18341 | MS.gene040398:CDS | 20.0% |
| !!! | TTGTTGTTTTTTCTTTCAAT+CGG | - | 51471:18547-18566 | MS.gene040398:intron | 20.0% |
| ! | ATGTTGAGAATTTACTTGAT+AGG | - | 51471:18948-18967 | MS.gene040398:intron | 25.0% |
| ! | ATGTTGGATAGGTTATATAT+TGG | + | 51471:18866-18885 | MS.gene040398:intergenic | 25.0% |
| ! | ATTGGTCAAAGTAAAATATG+TGG | + | 51471:18064-18083 | MS.gene040398:intergenic | 25.0% |
| ! | GACAATTTCTTATGTTACAT+TGG | + | 51471:17882-17901 | MS.gene040398:intergenic | 25.0% |
| ! | GATTATAACATAATGCACTA+TGG | - | 51471:18827-18846 | MS.gene040398:intron | 25.0% |
| ! | TAGCAGAATTTAATCCTAAA+GGG | - | 51471:17840-17859 | MS.gene040398:intron | 25.0% |
| ! | TAGTGCATTATGTTATAATC+TGG | + | 51471:18827-18846 | MS.gene040398:intergenic | 25.0% |
| ! | TTGGTCAAAGTAAAATATGT+GGG | + | 51471:18063-18082 | MS.gene040398:intergenic | 25.0% |
| !! | ACAACAATTTTTACCTGAAT+TGG | + | 51471:18135-18154 | MS.gene040398:intergenic | 25.0% |
| !! | CTATTCTATTTGTCTAAAAC+TGG | - | 51471:17438-17457 | MS.gene040398:CDS | 25.0% |
| !! | TTTATTTGTGTTACTGACTA+AGG | - | 51471:18725-18744 | MS.gene040398:CDS | 25.0% |
| !!! | AATCGATTGAATTTTGAGAA+GGG | + | 51471:18599-18618 | MS.gene040398:intergenic | 25.0% |
| !!! | TCGTGATTATTGTGTTATTT+TGG | - | 51471:18249-18268 | MS.gene040398:intron | 25.0% |
| AAACAGATCACTACACTATT+TGG | - | 51471:17815-17834 | MS.gene040398:intron | 30.0% | |
| AAATAGTGTAGTGATCTGTT+TGG | + | 51471:17816-17835 | MS.gene040398:intergenic | 30.0% | |
| AACACATAATCAAAAACAGC+AGG | + | 51471:17260-17279 | MS.gene040398:intergenic | 30.0% | |
| AACATCGAAGAATGTATTGA+TGG | - | 51471:18628-18647 | MS.gene040398:intron | 30.0% | |
| AAGGATTTATTCTGTGTACT+CGG | + | 51471:18494-18513 | MS.gene040398:intergenic | 30.0% | |
| ATCAACATCAACAAAGAACA+TGG | - | 51471:18199-18218 | MS.gene040398:intron | 30.0% | |
| ATTATGTGTTTAGCCAAACT+GGG | - | 51471:17270-17289 | MS.gene040398:CDS | 30.0% | |
| CACGAGTTAAATCTTACTTT+GGG | + | 51471:18234-18253 | MS.gene040398:intergenic | 30.0% | |
| CTAGCAGAATTTAATCCTAA+AGG | - | 51471:17839-17858 | MS.gene040398:intron | 30.0% | |
| CTTCTCAAAATTCAATCGAT+TGG | - | 51471:18598-18617 | MS.gene040398:intron | 30.0% | |
| GCACAAAAACATTGTATTGA+AGG | + | 51471:17736-17755 | MS.gene040398:intergenic | 30.0% | |
| TACTTCTCTTATCAACATCA+TGG | + | 51471:18982-19001 | MS.gene040398:intergenic | 30.0% | |
| TCAAAATTCAATCGATTGGA+AGG | - | 51471:18602-18621 | MS.gene040398:intron | 30.0% | |
| TCACGAGTTAAATCTTACTT+TGG | + | 51471:18235-18254 | MS.gene040398:intergenic | 30.0% | |
| TGGATAGGTTATATATTGGT+GGG | + | 51471:18862-18881 | MS.gene040398:intergenic | 30.0% | |
| TTGGATAGGTTATATATTGG+TGG | + | 51471:18863-18882 | MS.gene040398:intergenic | 30.0% | |
| TTTATGTACCTCACCAATAA+AGG | + | 51471:17611-17630 | MS.gene040398:intergenic | 30.0% | |
| ! | AATCAATTTTCCTTCAGAGA+AGG | + | 51471:17503-17522 | MS.gene040398:intergenic | 30.0% |
| ! | ACAATTTTTACCTGAATTGG+TGG | + | 51471:18132-18151 | MS.gene040398:intergenic | 30.0% |
| ! | TCTATTTGTCTAAAACTGGA+AGG | - | 51471:17442-17461 | MS.gene040398:CDS | 30.0% |
| ! | TTCTTTGTTGATGTTGATTG+AGG | + | 51471:18197-18216 | MS.gene040398:intergenic | 30.0% |
| !! | CAATCGATTGAATTTTGAGA+AGG | + | 51471:18600-18619 | MS.gene040398:intergenic | 30.0% |
| AAAAACAGCAGGTAGATCAT+AGG | + | 51471:17249-17268 | MS.gene040397:intergenic | 35.0% | |
| AATTAATCTTCTGCCCAGTT+TGG | + | 51471:17286-17305 | MS.gene040398:intergenic | 35.0% | |
| ATGAAGATAGGTAGATCATG+TGG | + | 51471:18890-18909 | MS.gene040398:intergenic | 35.0% | |
| ATGTGGCTTAGATAAGCAAT+AGG | + | 51471:17556-17575 | MS.gene040398:intergenic | 35.0% | |
| CTACCTATCTTCATTAGCTA+TGG | - | 51471:18896-18915 | MS.gene040398:intron | 35.0% | |
| CTTCTATGTTCTTACCCTTT+AGG | + | 51471:17857-17876 | MS.gene040398:intergenic | 35.0% | |
| GATTATGTGTTTAGCCAAAC+TGG | - | 51471:17269-17288 | MS.gene040398:CDS | 35.0% | |
| TACCTCACCAATAAAGGATT+TGG | + | 51471:17605-17624 | MS.gene040398:intergenic | 35.0% | |
| TGTGGCTTAGATAAGCAATA+GGG | + | 51471:17555-17574 | MS.gene040398:intergenic | 35.0% | |
| ! | CTCATGCTGATTACATTATG+GGG | - | 51471:19023-19042 | MS.gene040398:CDS | 35.0% |
| ! | GCTCATGCTGATTACATTAT+GGG | - | 51471:19022-19041 | MS.gene040398:CDS | 35.0% |
| ! | TGCTCATGCTGATTACATTA+TGG | - | 51471:19021-19040 | MS.gene040398:CDS | 35.0% |
| !! | GAATTTTGAGAAGGGAGTTA+GGG | + | 51471:18591-18610 | MS.gene040398:intergenic | 35.0% |
| !! | TGAATTTTGAGAAGGGAGTT+AGG | + | 51471:18592-18611 | MS.gene040398:intergenic | 35.0% |
| AACAAAGAACATGGTGCACT+TGG | - | 51471:18208-18227 | MS.gene040398:intron | 40.0% | |
| AACAATGTCCGTGTTTCACT+TGG | + | 51471:17675-17694 | MS.gene040398:intergenic | 40.0% | |
| AACTATGCAGTTGTTAGACG+TGG | - | 51471:18773-18792 | MS.gene040398:CDS | 40.0% | |
| AAGTCGTAGCAGTCAATTCT+AGG | + | 51471:18010-18029 | MS.gene040398:intergenic | 40.0% | |
| ACTATGCAGTTGTTAGACGT+GGG | - | 51471:18774-18793 | MS.gene040398:CDS | 40.0% | |
| AGCACATAGAGACTTGAGAA+AGG | + | 51471:17984-18003 | MS.gene040398:intergenic | 40.0% | |
| AGCCAAATCCTTTATTGGTG+AGG | - | 51471:17600-17619 | MS.gene040398:intron | 40.0% | |
| AGGTAGATCATGTGGAATGT+TGG | + | 51471:18882-18901 | MS.gene040398:intergenic | 40.0% | |
| AGTCGTAGCAGTCAATTCTA+GGG | + | 51471:18009-18028 | MS.gene040398:intergenic | 40.0% | |
| CCTATCTTCATTAGCTATGG+AGG | - | 51471:18899-18918 | MS.gene040398:intron | 40.0% | |
| CCTCCATAGCTAATGAAGAT+AGG | + | 51471:18902-18921 | MS.gene040398:intergenic | 40.0% | |
| GATCATGTGGAATGTTGGAT+AGG | + | 51471:18877-18896 | MS.gene040398:intergenic | 40.0% | |
| GGGCAGAAGATTAATTACGT+TGG | - | 51471:17290-17309 | MS.gene040398:CDS | 40.0% | |
| GTTGCAGCCAAATCCTTTAT+TGG | - | 51471:17595-17614 | MS.gene040398:intron | 40.0% | |
| TAAAACTGGAAGGCTGTGTT+TGG | - | 51471:17452-17471 | MS.gene040398:CDS | 40.0% | |
| TCATCACACCAAGTGAAACA+CGG | - | 51471:17664-17683 | MS.gene040398:intron | 40.0% | |
| TTCGTAAATGCCACCAATTC+AGG | - | 51471:18119-18138 | MS.gene040398:CDS | 40.0% | |
| TTTGAGTGTCCGACACATAT+TGG | + | 51471:17709-17728 | MS.gene040398:intergenic | 40.0% | |
| ! | AACACGGACATTGTTTCTCT+CGG | - | 51471:17680-17699 | MS.gene040398:intron | 40.0% |
| ! | GACTTGATGACTGCATTAAC+TGG | - | 51471:18025-18044 | MS.gene040398:intron | 40.0% |
| !! | ATAAAGCTGCTGATTTCAGC+TGG | + | 51471:17528-17547 | MS.gene040398:intergenic | 40.0% |
| CTATGCAGTTGTTAGACGTG+GGG | - | 51471:18775-18794 | MS.gene040398:CDS | 45.0% | |
| GTCCCAAGACCAATTCCAAA+AGG | + | 51471:17216-17235 | MS.gene040398:intergenic | 45.0% | |
| TAATTACGTTGGCCACTCTC+TGG | - | 51471:17301-17320 | MS.gene040398:CDS | 45.0% | |
| TCTCGGACACCAATATGTGT+CGG | - | 51471:17697-17716 | MS.gene040398:intron | 45.0% | |
| ! | AGTAATGGAGTACCAGAGAG+TGG | + | 51471:17316-17335 | MS.gene040398:intergenic | 45.0% |
| ! | GCTTTAGCTTCCTTCTCTGA+AGG | - | 51471:17490-17509 | MS.gene040398:CDS | 45.0% |
| !! | GACTTGAGAAAGGCATCAAC+AGG | + | 51471:17974-17993 | MS.gene040398:intergenic | 45.0% |
| !! | AATATATAAAATATTAATTG+GGG | + | 51471:17921-17940 | MS.gene040398:intergenic | 5.0% |
| !! | ATTATAATTGAAATATTTAA+AGG | + | 51471:18513-18532 | MS.gene040398:intergenic | 5.0% |
| !! | TTAAATATTTCAATTATAAT+TGG | - | 51471:18513-18532 | MS.gene040398:intron | 5.0% |
| AGAAGGGAGTTAGGGCACAT+TGG | + | 51471:18583-18602 | MS.gene040398:intergenic | 50.0% | |
| AGCCACATGAACACTGCACT+TGG | - | 51471:17568-17587 | MS.gene040398:intron | 50.0% | |
| CACCAAGTGCAGTGTTCATG+TGG | + | 51471:17573-17592 | MS.gene040398:intergenic | 50.0% | |
| ACTGGAAGGCTGTGTTTGGC+AGG | - | 51471:17456-17475 | MS.gene040398:CDS | 55.0% | |
| CTGGAAGGCTGTGTTTGGCA+GGG | - | 51471:17457-17476 | MS.gene040398:CDS | 55.0% | |
| GGGAGTTAGGGCACATTGGT+CGG | + | 51471:18579-18598 | MS.gene040398:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51471 | gene | 17212 | 19108 | 17212 | ID=MS.gene040398 |
| 51471 | mRNA | 17212 | 19108 | 17212 | ID=MS.gene040398.t1;Parent=MS.gene040398 |
| 51471 | exon | 18998 | 19108 | 18998 | ID=MS.gene040398.t1.exon1;Parent=MS.gene040398.t1 |
| 51471 | CDS | 18998 | 19108 | 18998 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| 51471 | exon | 18699 | 18842 | 18699 | ID=MS.gene040398.t1.exon2;Parent=MS.gene040398.t1 |
| 51471 | CDS | 18699 | 18842 | 18699 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| 51471 | exon | 18459 | 18499 | 18459 | ID=MS.gene040398.t1.exon3;Parent=MS.gene040398.t1 |
| 51471 | CDS | 18459 | 18499 | 18459 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| 51471 | exon | 18274 | 18353 | 18274 | ID=MS.gene040398.t1.exon4;Parent=MS.gene040398.t1 |
| 51471 | CDS | 18274 | 18353 | 18274 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| 51471 | exon | 18084 | 18179 | 18084 | ID=MS.gene040398.t1.exon5;Parent=MS.gene040398.t1 |
| 51471 | CDS | 18084 | 18179 | 18084 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| 51471 | exon | 17212 | 17537 | 17212 | ID=MS.gene040398.t1.exon6;Parent=MS.gene040398.t1 |
| 51471 | CDS | 17212 | 17537 | 17212 | ID=cds.MS.gene040398.t1;Parent=MS.gene040398.t1 |
| Gene Sequence |
| Protein sequence |
