AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04738

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04738.t1 MTR_8g103030 98.635 293 4 0 1 293 1 293 0.0 593
MS.gene04738.t1 MTR_6g488150 60.993 282 58 5 23 293 14 254 2.67e-108 315
MS.gene04738.t1 MTR_2g101500 54.627 335 101 11 1 293 1 326 2.49e-104 307
MS.gene04738.t1 MTR_5g030710 51.274 314 103 10 12 293 40 335 2.81e-95 285
MS.gene04738.t1 MTR_8g067530 50.482 311 107 9 12 293 64 356 1.08e-90 273
MS.gene04738.t1 MTR_5g067350 47.205 322 105 8 12 293 51 347 1.39e-82 253
MS.gene04738.t1 MTR_1g080860 48.214 280 92 8 24 292 15 252 1.90e-72 223
MS.gene04738.t1 MTR_1g093240 47.636 275 95 6 23 293 7 236 4.12e-70 217
MS.gene04738.t1 MTR_7g096090 63.125 160 55 2 135 293 94 250 2.87e-66 207
MS.gene04738.t1 MTR_1g080860 46.667 270 91 8 24 282 15 242 7.92e-65 204
MS.gene04738.t1 MTR_1g070520 62.424 165 51 4 135 292 84 244 1.08e-64 203
MS.gene04738.t1 MTR_1g093240 46.008 263 93 6 23 281 7 224 1.50e-61 195
MS.gene04738.t1 MTR_6g488150 52.709 203 45 5 23 214 14 176 4.12e-56 179
MS.gene04738.t1 MTR_1g093240 42.798 243 90 6 23 261 7 204 1.54e-49 163
MS.gene04738.t1 MTR_1g070830 45.385 260 70 11 24 281 12 201 8.91e-49 161
MS.gene04738.t1 MTR_1g076270 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene04738.t1 MTR_1g093350 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene04738.t1 MTR_1g040675 41.473 258 75 9 24 281 1 182 5.85e-45 150
MS.gene04738.t1 MTR_1g069495 43.169 183 71 3 139 288 81 263 1.73e-37 134
MS.gene04738.t1 MTR_5g067350 38.272 243 89 7 12 214 51 272 6.20e-35 127
MS.gene04738.t1 MTR_1g040675 38.235 238 71 8 24 261 1 162 8.42e-35 124
MS.gene04738.t1 MTR_2g100780 42.442 172 76 5 136 284 92 263 1.20e-32 121
MS.gene04738.t1 MTR_4g011880 49.333 150 45 4 138 285 52 172 2.19e-32 118
MS.gene04738.t1 MTR_2g100780 42.945 163 72 4 136 277 92 254 2.43e-32 120
MS.gene04738.t1 MTR_2g102490 53.636 110 38 3 170 276 84 183 2.48e-31 116
MS.gene04738.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 4.96e-29 112
MS.gene04738.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 5.33e-29 112
MS.gene04738.t1 MTR_3g106850 30.921 152 79 4 131 277 79 209 2.45e-19 84.7
MS.gene04738.t1 MTR_4g124300 35.811 148 63 5 135 276 61 182 5.12e-18 80.5
MS.gene04738.t1 MTR_7g110790 36.090 133 59 4 146 274 45 155 1.97e-16 75.5
MS.gene04738.t1 MTR_1g085750 37.607 117 57 2 165 281 76 176 2.82e-16 75.5
MS.gene04738.t1 MTR_4g115075 30.769 143 62 3 141 275 93 206 4.40e-14 70.1
MS.gene04738.t1 MTR_1g109510 28.571 161 85 4 114 274 38 168 9.88e-12 62.8
MS.gene04738.t1 MTR_1g085750 36.082 97 47 1 165 261 76 157 3.99e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04738.t1 AT4G29080 55.864 324 91 11 1 293 3 305 8.04e-102 300
MS.gene04738.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene04738.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene04738.t1 AT2G22670 48.993 298 97 7 24 292 64 335 3.19e-84 256
MS.gene04738.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene04738.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene04738.t1 AT5G65670 46.128 297 110 6 24 292 61 335 6.07e-74 230
MS.gene04738.t1 AT5G65670 45.819 299 110 7 24 292 61 337 4.98e-72 225
MS.gene04738.t1 AT4G14550 48.913 276 87 9 24 293 1 228 5.46e-70 216
MS.gene04738.t1 AT3G04730 53.778 225 76 5 93 292 14 235 1.91e-69 215
MS.gene04738.t1 AT3G23050 49.301 286 81 12 24 293 6 243 1.61e-68 213
MS.gene04738.t1 AT1G04250 45.956 272 100 6 21 292 4 228 1.51e-65 205
MS.gene04738.t1 AT4G14550 47.727 264 84 9 24 281 1 216 7.32e-63 198
MS.gene04738.t1 AT4G14550 44.444 243 81 9 24 260 1 195 1.22e-49 163
MS.gene04738.t1 AT3G23050 45.059 253 76 11 24 261 6 210 1.03e-48 161
MS.gene04738.t1 AT1G04240 41.313 259 75 10 24 282 5 186 9.95e-47 155
MS.gene04738.t1 AT1G04240 41.473 258 74 10 24 281 105 285 2.87e-45 155
MS.gene04738.t1 AT5G43700 52.667 150 47 6 133 282 57 182 2.44e-42 144
MS.gene04738.t1 AT3G23030 48.993 149 45 2 133 281 53 170 4.11e-39 135
MS.gene04738.t1 AT2G33310 44.068 177 69 4 133 287 69 237 6.25e-39 137
MS.gene04738.t1 AT3G15540 37.313 268 91 9 23 288 5 197 1.06e-38 135
MS.gene04738.t1 AT3G23030 48.993 149 45 2 133 281 140 257 1.79e-38 136
MS.gene04738.t1 AT1G04550 45.946 185 65 7 133 293 63 236 4.79e-38 134
MS.gene04738.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene04738.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene04738.t1 AT1G52830 36.726 226 100 6 56 281 2 184 1.04e-36 129
MS.gene04738.t1 AT4G14560 49.020 153 49 5 126 278 41 164 3.00e-35 125
MS.gene04738.t1 AT4G28640 44.910 167 70 4 133 282 81 242 7.41e-35 126
MS.gene04738.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.17e-34 126
MS.gene04738.t1 AT3G16500 40.964 166 75 4 130 277 92 252 2.24e-34 126
MS.gene04738.t1 AT3G16500 40.964 166 75 4 130 277 92 252 3.01e-34 125
MS.gene04738.t1 AT1G15580 44.366 142 49 2 135 276 48 159 4.69e-34 122
MS.gene04738.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.43e-33 124
MS.gene04738.t1 AT1G04100 41.916 167 68 4 142 281 95 259 6.05e-31 117
MS.gene04738.t1 AT5G25890 36.250 160 74 3 125 284 39 170 1.25e-29 110
MS.gene04738.t1 AT1G51950 42.683 164 67 6 136 277 92 250 3.91e-29 112
MS.gene04738.t1 AT4G14550 40.513 195 65 8 24 212 1 150 3.96e-28 107
MS.gene04738.t1 AT3G23050 41.429 210 60 11 24 218 6 167 1.84e-27 105
MS.gene04738.t1 AT3G17600 37.931 145 64 5 130 274 36 154 9.61e-23 92.4
MS.gene04738.t1 AT2G46990 39.423 104 47 1 173 276 85 172 7.18e-19 82.4
MS.gene04738.t1 AT3G62100 38.393 112 48 3 169 275 74 169 1.11e-18 82.0
MS.gene04738.t1 AT2G46990 40.449 89 37 1 173 261 85 157 2.51e-14 69.7
MS.gene04738.t1 AT3G62100 37.755 98 40 3 169 261 74 155 7.19e-14 68.2
MS.gene04738.t1 AT4G32280 35.294 102 45 2 173 274 118 198 4.25e-13 67.4
MS.gene04738.t1 AT4G32280 35.294 102 45 2 173 274 160 240 1.17e-12 67.0
MS.gene04738.t1 AT1G15050 31.579 133 74 2 147 279 35 150 5.89e-12 63.2
MS.gene04738.t1 AT1G15050 31.579 133 74 2 147 279 67 182 1.31e-11 62.8
MS.gene04738.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8
MS.gene04738.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8

Find 81 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACCAATGCTTCTCTCTT+TGG 0.185471 4.2:-2387816 None:intergenic
CTCTCTTTGGTTTCCATTGA+AGG 0.229858 4.2:-2387803 None:intergenic
TCACCACCACCTTTAGCTTT+AGG 0.284997 4.2:-2388043 None:intergenic
GCTATTACTGATGGAGGTTC+TGG 0.289483 4.2:+2387953 MS.gene04738:CDS
AGATCCACTTTCCTCAAGTA+TGG 0.291849 4.2:-2389338 None:intergenic
GGCCTCCAATCCGATCTTTC+CGG 0.299985 4.2:+2389222 MS.gene04738:CDS
GAGTCACCAGAGAGAGAAAA+TGG 0.324653 4.2:+2387881 MS.gene04738:CDS
GCCTCAGTTCAGTAGCTTTG+AGG 0.337674 4.2:-2387841 None:intergenic
CCAGGTCAATGTGGCTCATA+TGG 0.347339 4.2:+2390049 MS.gene04738:intron
GTGAAACCATTGAGTTCTTC+CGG 0.369117 4.2:-2389241 None:intergenic
ATGAAGAGTTCTGAAGCAAT+TGG 0.391710 4.2:+2390402 MS.gene04738:CDS
AGCATCAGAGAAACCCCTTT+TGG 0.393944 4.2:-2387933 None:intergenic
TTGTTATCATGCAAAGGCTT+TGG 0.395919 4.2:-2388130 None:intergenic
TTCTCTGATGCTATTACTGA+TGG 0.411709 4.2:+2387944 MS.gene04738:CDS
TGGTGGTGTGCTTAAGAGTT+TGG 0.414516 4.2:+2387901 MS.gene04738:CDS
TTATGGAGAGGTTCCTTCAA+TGG 0.424280 4.2:+2387790 MS.gene04738:CDS
GTGCCAGTGGAACAAGGTTA+TGG 0.429339 4.2:+2387773 MS.gene04738:CDS
CTGATGGAGGTTCTGGGAAG+TGG 0.429765 4.2:+2387960 MS.gene04738:CDS
CTCTCTGGTGACTCTGAGCC+TGG 0.432328 4.2:-2387872 None:intergenic
CCGAGTCTTGCAAGAGGTTG+AGG 0.432404 4.2:+2390376 MS.gene04738:CDS
ATCACCATCCTTGTCTTCAT+AGG 0.451715 4.2:-2390146 None:intergenic
ACAACAACTGAAGCAGAGAA+AGG 0.456026 4.2:-2388085 None:intergenic
AGATTGGTGGATCTCCTTCA+TGG 0.457760 4.2:+2390106 MS.gene04738:CDS
CGAATCAGGGCGCAAGTTGT+GGG 0.462809 4.2:+2389197 MS.gene04738:intron
CTATTACTGATGGAGGTTCT+GGG 0.466403 4.2:+2387954 MS.gene04738:CDS
TGCTAGTTGGTGATGTTCCA+TGG 0.467311 4.2:+2390173 MS.gene04738:CDS
TTTGGTGTCTGGTGCCAAAA+GGG 0.469379 4.2:+2387919 MS.gene04738:CDS
TACATATATGCATTCAGACT+TGG 0.476242 4.2:-2389294 None:intergenic
TGGAAATGGTGGATCTGAAG+TGG 0.482511 4.2:+2387991 MS.gene04738:CDS
GCGAATCAGGGCGCAAGTTG+TGG 0.486260 4.2:+2389196 MS.gene04738:intron
GTGCTTAAGAGTTTGGTGTC+TGG 0.488896 4.2:+2387908 MS.gene04738:CDS
TGAAGAGTTCTGAAGCAATT+GGG 0.489200 4.2:+2390403 MS.gene04738:CDS
GTTTGGTGTCTGGTGCCAAA+AGG 0.490914 4.2:+2387918 MS.gene04738:CDS
GAACTGAGGCTAGGGTTACC+AGG 0.498587 4.2:+2387854 MS.gene04738:CDS
AAATCTAAGTGAAAGTAGAT+TGG 0.505003 4.2:+2390090 MS.gene04738:CDS
TTGTTCTCTCCTAAAGCTAA+AGG 0.505328 4.2:+2388034 MS.gene04738:CDS
TGATGGAGGTTCTGGGAAGT+GGG 0.506160 4.2:+2387961 MS.gene04738:CDS
GAAACCAAAGAGAGAAGCAT+TGG 0.506436 4.2:+2387812 MS.gene04738:CDS
ATCTTTCCGGAAGAACTCAA+TGG 0.508429 4.2:+2389235 MS.gene04738:CDS
TTGAGTTCTTCCGGAAAGAT+CGG 0.510793 4.2:-2389232 None:intergenic
ATGAAGACAAGGATGGTGAT+TGG 0.522716 4.2:+2390149 MS.gene04738:CDS
CAACAACTGAAGCAGAGAAA+GGG 0.523720 4.2:-2388084 None:intergenic
GGAAATGGTGGATCTGAAGT+GGG 0.526580 4.2:+2387992 MS.gene04738:CDS
TCTGATGCTATTACTGATGG+AGG 0.531086 4.2:+2387947 MS.gene04738:CDS
TTCCGGAAAGATCGGATTGG+AGG 0.531139 4.2:-2389224 None:intergenic
TCCTGCAGCACCTCGAGCCA+TGG 0.531399 4.2:+2390508 MS.gene04738:intron
AAGAAATCTTACTTTGCAGA+AGG 0.533764 4.2:-2388169 None:intergenic
TTGAGGAAAGTGGATCTGAA+TGG 0.538432 4.2:+2389344 MS.gene04738:CDS
TATGTAAAAGTCAGCATGGA+AGG 0.544619 4.2:+2389311 MS.gene04738:CDS
AAATCTTACTTTGCAGAAGG+AGG 0.546311 4.2:-2388166 None:intergenic
TCTAAGTGAAAGTAGATTGG+TGG 0.547921 4.2:+2390093 MS.gene04738:CDS
TCTCCATAACCTTGTTCCAC+TGG 0.551400 4.2:-2387776 None:intergenic
AAAGCTACTGAACTGAGGCT+AGG 0.551933 4.2:+2387845 MS.gene04738:CDS
AGTTCTGAAGCAATTGGGCT+AGG 0.552508 4.2:+2390408 MS.gene04738:CDS
TGCTCCATACTTGAGGAAAG+TGG 0.552637 4.2:+2389334 MS.gene04738:CDS
AAGCTACTGAACTGAGGCTA+GGG 0.557212 4.2:+2387846 MS.gene04738:CDS
TGTGGTTTGTTATCATGCAA+AGG 0.558132 4.2:-2388136 None:intergenic
GAAGTGGGGTTGTGTAAAGA+TGG 0.559129 4.2:+2388007 MS.gene04738:CDS
TTCTTCCGGAAAGATCGGAT+TGG 0.561734 4.2:-2389227 None:intergenic
TTGGTGTCTGGTGCCAAAAG+GGG 0.565847 4.2:+2387920 MS.gene04738:CDS
AGAAAATAGACGTACTCCCA+TGG 0.566126 4.2:-2390190 None:intergenic
CATATATGTAAAAGTCAGCA+TGG 0.572072 4.2:+2389307 MS.gene04738:CDS
GCTAGTTGGTGATGTTCCAT+GGG 0.572324 4.2:+2390174 MS.gene04738:CDS
TCTCCTAAAGCTAAAGGTGG+TGG 0.583056 4.2:+2388040 MS.gene04738:CDS
CCTCAACCTCTTGCAAGACT+CGG 0.585897 4.2:-2390376 None:intergenic
TTACATTTCTCCATGGCTCG+AGG 0.588053 4.2:-2390518 None:intergenic
TCAGGGCGCAAGTTGTGGGA+TGG 0.592117 4.2:+2389201 MS.gene04738:intron
GCACATATTCTGAACCATGA+AGG 0.604244 4.2:-2390120 None:intergenic
GATGGTGATTGGATGCTAGT+TGG 0.610602 4.2:+2390160 MS.gene04738:CDS
TGGAAGGTGCTCCATACTTG+AGG 0.612400 4.2:+2389327 MS.gene04738:CDS
GCAGAAGGAGGAGAAACATG+TGG 0.623815 4.2:-2388154 None:intergenic
ATGTCTGTGCCAGTGGAACA+AGG 0.641481 4.2:+2387767 MS.gene04738:CDS
TTCTCTCCTAAAGCTAAAGG+TGG 0.652446 4.2:+2388037 MS.gene04738:CDS
TTGACAAATCCAGGTCAATG+TGG 0.662027 4.2:+2390040 MS.gene04738:intron
TGTGCTTACCTATGAAGACA+AGG 0.670147 4.2:+2390138 MS.gene04738:CDS
TGTTCACCGAGTCTTGCAAG+AGG 0.674237 4.2:+2390370 MS.gene04738:CDS
GAAATGGTGGATCTGAAGTG+GGG 0.680430 4.2:+2387993 MS.gene04738:CDS
CTTACCTATGAAGACAAGGA+TGG 0.683773 4.2:+2390142 MS.gene04738:CDS
AGTGGAACAAGGTTATGGAG+AGG 0.703860 4.2:+2387778 MS.gene04738:CDS
TCACCAGAGAGAGAAAATGG+TGG 0.713194 4.2:+2387884 MS.gene04738:CDS
ACCTCAAAGCTACTGAACTG+AGG 0.714271 4.2:+2387840 MS.gene04738:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTTAAGTTTAACATT+TGG - chr4.2:2389690-2389709 None:intergenic 15.0%
!!! GATGTTATTTTTGTTATTTT+AGG + chr4.2:2390346-2390365 MS.gene04738:intron 15.0%
!!! GATTTTAATATAACAAACAT+TGG - chr4.2:2390439-2390458 None:intergenic 15.0%
!!! TCATTTTATTTAAGCAAAAA+AGG - chr4.2:2390003-2390022 None:intergenic 15.0%
!! AAGTAAGATTTCTTCTTATT+TGG + chr4.2:2388178-2388197 MS.gene04738:intron 20.0%
!!! ACTTGTTGTATCTTTTATTA+TGG - chr4.2:2388587-2388606 None:intergenic 20.0%
!!! ATTGATAGTTAGACTTATTT+TGG + chr4.2:2390269-2390288 MS.gene04738:intron 20.0%
!!! TGATTTCTTTTTTCTCAATA+CGG + chr4.2:2389166-2389185 MS.gene04738:intron 20.0%
! AAATCTAAGTGAAAGTAGAT+TGG + chr4.2:2390090-2390109 MS.gene04738:CDS 25.0%
! AACATGCAGAAGAAAATTTA+AGG - chr4.2:2388920-2388939 None:intergenic 25.0%
! AGATTTATATTATCATGCGT+TGG - chr4.2:2389096-2389115 None:intergenic 25.0%
! CGATCATAAATTCAAAACTA+AGG - chr4.2:2389133-2389152 None:intergenic 25.0%
! GCAAAAATATTTGAAAATCG+AGG + chr4.2:2389579-2389598 MS.gene04738:intron 25.0%
! TGACATGAATTTATGAACAT+CGG + chr4.2:2389764-2389783 MS.gene04738:intron 25.0%
! TTGATTCTAACATTGTTCAT+AGG - chr4.2:2390295-2390314 None:intergenic 25.0%
!! TCTATTGTTGGTTGAAATTA+TGG + chr4.2:2389858-2389877 MS.gene04738:intron 25.0%
!!! ACTTTTTTCACAAGCTATTA+TGG + chr4.2:2388642-2388661 MS.gene04738:intron 25.0%
!!! AGTTTTGAATTTATGATCGT+TGG + chr4.2:2389134-2389153 MS.gene04738:intron 25.0%
!!! GATACCTTCATATTTTTTGT+CGG + chr4.2:2390467-2390486 MS.gene04738:intron 25.0%
!!! TTCACATAGATAGTTACTTT+TGG + chr4.2:2388889-2388908 MS.gene04738:intron 25.0%
!!! TTCTACATTAATTTGTCAGT+TGG + chr4.2:2388412-2388431 MS.gene04738:intron 25.0%
AAGAAATCTTACTTTGCAGA+AGG - chr4.2:2388172-2388191 None:intergenic 30.0%
AGATATTTGTGATCATGCAT+CGG - chr4.2:2389492-2389511 None:intergenic 30.0%
CATATATGTAAAAGTCAGCA+TGG + chr4.2:2389307-2389326 MS.gene04738:CDS 30.0%
TACATATATGCATTCAGACT+TGG - chr4.2:2389297-2389316 None:intergenic 30.0%
TATGTGCTCTAGTAAATTTC+AGG + chr4.2:2388840-2388859 MS.gene04738:intron 30.0%
TCAAATAAGCCAATCCAAAT+AGG + chr4.2:2388744-2388763 MS.gene04738:intron 30.0%
TCAACCAACAATAGAGAAAA+TGG - chr4.2:2389853-2389872 None:intergenic 30.0%
TGCATGTATTTATATGTCAC+AGG + chr4.2:2389904-2389923 MS.gene04738:intron 30.0%
! TATATTCTTATGTTAGCCTC+TGG + chr4.2:2388787-2388806 MS.gene04738:intron 30.0%
! TCAACCATTTTCTCTATTGT+TGG + chr4.2:2389846-2389865 MS.gene04738:intron 30.0%
!! ATGTTTTTGTTGACAAATCC+AGG + chr4.2:2390031-2390050 MS.gene04738:intron 30.0%
!!! AATCACTTTTGAGACTGTTT+GGG - chr4.2:2388708-2388727 None:intergenic 30.0%
!!! GAATGGTTTTAGCTCTTATA+GGG + chr4.2:2389361-2389380 MS.gene04738:CDS 30.0%
!!! TAATCACTTTTGAGACTGTT+TGG - chr4.2:2388709-2388728 None:intergenic 30.0%
!!! TGAATGGTTTTAGCTCTTAT+AGG + chr4.2:2389360-2389379 MS.gene04738:CDS 30.0%
AAATCTTACTTTGCAGAAGG+AGG - chr4.2:2388169-2388188 None:intergenic 35.0%
ACATCAAGACACACAGATAA+TGG - chr4.2:2389464-2389483 None:intergenic 35.0%
ATAATTAGCAACAACCACCA+AGG - chr4.2:2388770-2388789 None:intergenic 35.0%
ATCTTCATTCTCAACCATGT+TGG - chr4.2:2389951-2389970 None:intergenic 35.0%
CAACTGTACTGTCAAAGTAT+AGG - chr4.2:2389740-2389759 None:intergenic 35.0%
CATCAAGACACACAGATAAT+GGG - chr4.2:2389463-2389482 None:intergenic 35.0%
GAGTCCGACAAAAAATATGA+AGG - chr4.2:2390474-2390493 None:intergenic 35.0%
GCATGATCACAAATATCTCT+CGG + chr4.2:2389494-2389513 MS.gene04738:intron 35.0%
TATGTAAAAGTCAGCATGGA+AGG + chr4.2:2389311-2389330 MS.gene04738:CDS 35.0%
TCTAAGTGAAAGTAGATTGG+TGG + chr4.2:2390093-2390112 MS.gene04738:CDS 35.0%
TTCTCTGATGCTATTACTGA+TGG + chr4.2:2387944-2387963 MS.gene04738:CDS 35.0%
TTGTTATCATGCAAAGGCTT+TGG - chr4.2:2388133-2388152 None:intergenic 35.0%
TTGTTCTCTCCTAAAGCTAA+AGG + chr4.2:2388034-2388053 MS.gene04738:CDS 35.0%
! ATACGGTTTTTATGCGAATC+AGG + chr4.2:2389183-2389202 MS.gene04738:intron 35.0%
! ATGGTTGAAAGAGAGTGTAT+TGG - chr4.2:2389834-2389853 None:intergenic 35.0%
! ATGTAGAAGATCTTCTAGAG+AGG - chr4.2:2388400-2388419 None:intergenic 35.0%
! CAAAGGTATGACTACTGTAT+AGG - chr4.2:2389976-2389995 None:intergenic 35.0%
! GAAAGAGAGTGTATTGGTAA+CGG - chr4.2:2389828-2389847 None:intergenic 35.0%
! TAAGCAAAAAAGGCATGCAA+AGG - chr4.2:2389993-2390012 None:intergenic 35.0%
! TACGGTTTTTATGCGAATCA+GGG + chr4.2:2389184-2389203 MS.gene04738:intron 35.0%
! TGTAGAAGATCTTCTAGAGA+GGG - chr4.2:2388399-2388418 None:intergenic 35.0%
! TGTGCTTTGTTGATTGATGT+TGG + chr4.2:2388239-2388258 MS.gene04738:intron 35.0%
!! ATGAAGAGTTCTGAAGCAAT+TGG + chr4.2:2390402-2390421 MS.gene04738:CDS 35.0%
!! TGAAGAGTTCTGAAGCAATT+GGG + chr4.2:2390403-2390422 MS.gene04738:CDS 35.0%
!! TGTGGTTTGTTATCATGCAA+AGG - chr4.2:2388139-2388158 None:intergenic 35.0%
AAATCGAGGTGTGTCTTGAA+TGG + chr4.2:2389593-2389612 MS.gene04738:intron 40.0%
AAGACATGGAAAAGGCTAAC+TGG - chr4.2:2389026-2389045 None:intergenic 40.0%
AATATCTCTCGGTGATGTGA+TGG + chr4.2:2389505-2389524 MS.gene04738:intron 40.0%
ACAACAACTGAAGCAGAGAA+AGG - chr4.2:2388088-2388107 None:intergenic 40.0%
ACTGGACAAAATCAAATCCC+AGG - chr4.2:2389008-2389027 None:intergenic 40.0%
AGAAAATAGACGTACTCCCA+TGG - chr4.2:2390193-2390212 None:intergenic 40.0%
AGATCCACTTTCCTCAAGTA+TGG - chr4.2:2389341-2389360 None:intergenic 40.0%
AGCATCAGAAGACATGGAAA+AGG - chr4.2:2389034-2389053 None:intergenic 40.0%
ATCACCATCCTTGTCTTCAT+AGG - chr4.2:2390149-2390168 None:intergenic 40.0%
ATCTTTCCGGAAGAACTCAA+TGG + chr4.2:2389235-2389254 MS.gene04738:CDS 40.0%
ATGAAGACAAGGATGGTGAT+TGG + chr4.2:2390149-2390168 MS.gene04738:CDS 40.0%
CAAACCAATGCTTCTCTCTT+TGG - chr4.2:2387819-2387838 None:intergenic 40.0%
CAACAACTGAAGCAGAGAAA+GGG - chr4.2:2388087-2388106 None:intergenic 40.0%
CTATTACTGATGGAGGTTCT+GGG + chr4.2:2387954-2387973 MS.gene04738:CDS 40.0%
CTGGACAAAATCAAATCCCA+GGG - chr4.2:2389007-2389026 None:intergenic 40.0%
CTTACCTATGAAGACAAGGA+TGG + chr4.2:2390142-2390161 MS.gene04738:CDS 40.0%
GCACATATTCTGAACCATGA+AGG - chr4.2:2390123-2390142 None:intergenic 40.0%
GTGAAACCATTGAGTTCTTC+CGG - chr4.2:2389244-2389263 None:intergenic 40.0%
TCTGATGCTATTACTGATGG+AGG + chr4.2:2387947-2387966 MS.gene04738:CDS 40.0%
TGTGCTTACCTATGAAGACA+AGG + chr4.2:2390138-2390157 MS.gene04738:CDS 40.0%
TTATGGAGAGGTTCCTTCAA+TGG + chr4.2:2387790-2387809 MS.gene04738:CDS 40.0%
TTCTCTCCTAAAGCTAAAGG+TGG + chr4.2:2388037-2388056 MS.gene04738:CDS 40.0%
TTGACAAATCCAGGTCAATG+TGG + chr4.2:2390040-2390059 MS.gene04738:intron 40.0%
TTGAGGAAAGTGGATCTGAA+TGG + chr4.2:2389344-2389363 MS.gene04738:CDS 40.0%
TTGAGTTCTTCCGGAAAGAT+CGG - chr4.2:2389235-2389254 None:intergenic 40.0%
! CTCTCTTTGGTTTCCATTGA+AGG - chr4.2:2387806-2387825 None:intergenic 40.0%
! GAAACCAAAGAGAGAAGCAT+TGG + chr4.2:2387812-2387831 MS.gene04738:CDS 40.0%
! GCATCAGCATCATTCTTTTG+AGG - chr4.2:2389269-2389288 None:intergenic 40.0%
! GCGACTTTTACATTTCTCCA+TGG - chr4.2:2390528-2390547 None:intergenic 40.0%
! TATGTCACAGGTTCTGATTG+TGG + chr4.2:2389916-2389935 MS.gene04738:intron 40.0%
!! AAGCAGAGAAAGGGTTTTGT+TGG - chr4.2:2388078-2388097 None:intergenic 40.0%
!! AAGTGGGTTTTGTCTGGAAA+TGG + chr4.2:2387977-2387996 MS.gene04738:CDS 40.0%
AAAGCTACTGAACTGAGGCT+AGG + chr4.2:2387845-2387864 MS.gene04738:CDS 45.0%
AAATCCCAGGGCATACACAA+GGG - chr4.2:2388995-2389014 None:intergenic 45.0%
AACAACCACCAAGGCCTATT+TGG - chr4.2:2388761-2388780 None:intergenic 45.0%
AAGCCAATCCAAATAGGCCT+TGG + chr4.2:2388750-2388769 MS.gene04738:intron 45.0%
AAGCTACTGAACTGAGGCTA+GGG + chr4.2:2387846-2387865 MS.gene04738:CDS 45.0%
ACCTCAAAGCTACTGAACTG+AGG + chr4.2:2387840-2387859 MS.gene04738:CDS 45.0%
AGTGGAACAAGGTTATGGAG+AGG + chr4.2:2387778-2387797 MS.gene04738:CDS 45.0%
CCATGTCTTCTGATGCTGAA+AGG + chr4.2:2389037-2389056 MS.gene04738:intron 45.0%
CCTTTCAGCATCAGAAGACA+TGG - chr4.2:2389040-2389059 None:intergenic 45.0%
GAAGTGGGGTTGTGTAAAGA+TGG + chr4.2:2388007-2388026 MS.gene04738:CDS 45.0%
GAGTCACCAGAGAGAGAAAA+TGG + chr4.2:2387881-2387900 MS.gene04738:CDS 45.0%
GATGGTGATTGGATGCTAGT+TGG + chr4.2:2390160-2390179 MS.gene04738:CDS 45.0%
GCTATTACTGATGGAGGTTC+TGG + chr4.2:2387953-2387972 MS.gene04738:CDS 45.0%
TCACCAGAGAGAGAAAATGG+TGG + chr4.2:2387884-2387903 MS.gene04738:CDS 45.0%
TCTCCATAACCTTGTTCCAC+TGG - chr4.2:2387779-2387798 None:intergenic 45.0%
TCTCCTAAAGCTAAAGGTGG+TGG + chr4.2:2388040-2388059 MS.gene04738:CDS 45.0%
TGCTCCATACTTGAGGAAAG+TGG + chr4.2:2389334-2389353 MS.gene04738:CDS 45.0%
TTACATTTCTCCATGGCTCG+AGG - chr4.2:2390521-2390540 None:intergenic 45.0%
TTAGCCTCTGGTGTAAAAGC+TGG + chr4.2:2388799-2388818 MS.gene04738:intron 45.0%
TTCTCAACCATGTTGGCACT+TGG - chr4.2:2389944-2389963 None:intergenic 45.0%
TTCTTCCGGAAAGATCGGAT+TGG - chr4.2:2389230-2389249 None:intergenic 45.0%
! ACACCACCATTTTCTCTCTC+TGG - chr4.2:2387890-2387909 None:intergenic 45.0%
! AGATTGGTGGATCTCCTTCA+TGG + chr4.2:2390106-2390125 MS.gene04738:CDS 45.0%
! AGCATCAGAGAAACCCCTTT+TGG - chr4.2:2387936-2387955 None:intergenic 45.0%
! TGGTGGTGTGCTTAAGAGTT+TGG + chr4.2:2387901-2387920 MS.gene04738:CDS 45.0%
!! ACTCACTTGTTGATTCGCAC+TGG + chr4.2:2388483-2388502 MS.gene04738:intron 45.0%
!! ACTTGTTGATTCGCACTGGT+TGG + chr4.2:2388487-2388506 MS.gene04738:intron 45.0%
!! AGTTCTGAAGCAATTGGGCT+AGG + chr4.2:2390408-2390427 MS.gene04738:CDS 45.0%
!! GAAATGGTGGATCTGAAGTG+GGG + chr4.2:2387993-2388012 MS.gene04738:CDS 45.0%
!! GCTAGTTGGTGATGTTCCAT+GGG + chr4.2:2390174-2390193 MS.gene04738:CDS 45.0%
!! GGAAATGGTGGATCTGAAGT+GGG + chr4.2:2387992-2388011 MS.gene04738:CDS 45.0%
!! GTGCTTAAGAGTTTGGTGTC+TGG + chr4.2:2387908-2387927 MS.gene04738:CDS 45.0%
!! TCACCACCACCTTTAGCTTT+AGG - chr4.2:2388046-2388065 None:intergenic 45.0%
!! TGCTAGTTGGTGATGTTCCA+TGG + chr4.2:2390173-2390192 MS.gene04738:CDS 45.0%
!! TGGAAATGGTGGATCTGAAG+TGG + chr4.2:2387991-2388010 MS.gene04738:CDS 45.0%
!! TGGGTTTTGTCTGGAAATGG+TGG + chr4.2:2387980-2387999 MS.gene04738:CDS 45.0%
!! TTTGGTGTCTGGTGCCAAAA+GGG + chr4.2:2387919-2387938 MS.gene04738:CDS 45.0%
ATGTCTGTGCCAGTGGAACA+AGG + chr4.2:2387767-2387786 MS.gene04738:CDS 50.0%
CAAATCCCAGGGCATACACA+AGG - chr4.2:2388996-2389015 None:intergenic 50.0%
CCAATCCAAATAGGCCTTGG+TGG + chr4.2:2388753-2388772 MS.gene04738:intron 50.0%
CCACCAAGGCCTATTTGGAT+TGG - chr4.2:2388756-2388775 None:intergenic 50.0%
CCAGGTCAATGTGGCTCATA+TGG + chr4.2:2390049-2390068 MS.gene04738:intron 50.0%
CCATATGAGCCACATTGACC+TGG - chr4.2:2390052-2390071 None:intergenic 50.0%
CCTCAACCTCTTGCAAGACT+CGG - chr4.2:2390379-2390398 None:intergenic 50.0%
GCAGAAGGAGGAGAAACATG+TGG - chr4.2:2388157-2388176 None:intergenic 50.0%
GTGCCAGTGGAACAAGGTTA+TGG + chr4.2:2387773-2387792 MS.gene04738:CDS 50.0%
TGTTCACCGAGTCTTGCAAG+AGG + chr4.2:2390370-2390389 MS.gene04738:CDS 50.0%
! AACGCCAGCTTTTACACCAG+AGG - chr4.2:2388806-2388825 None:intergenic 50.0%
! GCCTCAGTTCAGTAGCTTTG+AGG - chr4.2:2387844-2387863 None:intergenic 50.0%
! TTCCGGAAAGATCGGATTGG+AGG - chr4.2:2389227-2389246 None:intergenic 50.0%
! TTGGTGTCTGGTGCCAAAAG+GGG + chr4.2:2387920-2387939 MS.gene04738:CDS 50.0%
!! GTTTGGTGTCTGGTGCCAAA+AGG + chr4.2:2387918-2387937 MS.gene04738:CDS 50.0%
!! TCTGGGAAGTGGGTTTTGTC+TGG + chr4.2:2387971-2387990 MS.gene04738:CDS 50.0%
!! TGATGGAGGTTCTGGGAAGT+GGG + chr4.2:2387961-2387980 MS.gene04738:CDS 50.0%
!! TGGAAGGTGCTCCATACTTG+AGG + chr4.2:2389327-2389346 MS.gene04738:CDS 50.0%
CCGAGTCTTGCAAGAGGTTG+AGG + chr4.2:2390376-2390395 MS.gene04738:CDS 55.0%
CGAATCAGGGCGCAAGTTGT+GGG + chr4.2:2389197-2389216 MS.gene04738:intron 55.0%
GAACTGAGGCTAGGGTTACC+AGG + chr4.2:2387854-2387873 MS.gene04738:CDS 55.0%
GGCCTCCAATCCGATCTTTC+CGG + chr4.2:2389222-2389241 MS.gene04738:CDS 55.0%
TGTGCCCTTGTGTATGCCCT+GGG + chr4.2:2388988-2389007 MS.gene04738:intron 55.0%
TTGTGCCCTTGTGTATGCCC+TGG + chr4.2:2388987-2389006 MS.gene04738:intron 55.0%
! TGTGGTGCCAAGTGCCAACA+TGG + chr4.2:2389934-2389953 MS.gene04738:intron 55.0%
!! CTGATGGAGGTTCTGGGAAG+TGG + chr4.2:2387960-2387979 MS.gene04738:CDS 55.0%
CTCTCTGGTGACTCTGAGCC+TGG - chr4.2:2387875-2387894 None:intergenic 60.0%
GCGAATCAGGGCGCAAGTTG+TGG + chr4.2:2389196-2389215 MS.gene04738:intron 60.0%
TCAGGGCGCAAGTTGTGGGA+TGG + chr4.2:2389201-2389220 MS.gene04738:intron 60.0%
TCCTGCAGCACCTCGAGCCA+TGG + chr4.2:2390508-2390527 MS.gene04738:intron 65.0%
!! TCCATGGCTCGAGGTGCTGC+AGG - chr4.2:2390512-2390531 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 2387767 2390553 2387767 ID=MS.gene04738
chr4.2 mRNA 2387767 2390553 2387767 ID=MS.gene04738.t1;Parent=MS.gene04738
chr4.2 exon 2387767 2388179 2387767 ID=MS.gene04738.t1.exon1;Parent=MS.gene04738.t1
chr4.2 CDS 2387767 2388179 2387767 ID=cds.MS.gene04738.t1;Parent=MS.gene04738.t1
chr4.2 exon 2389205 2389428 2389205 ID=MS.gene04738.t1.exon2;Parent=MS.gene04738.t1
chr4.2 CDS 2389205 2389428 2389205 ID=cds.MS.gene04738.t1;Parent=MS.gene04738.t1
chr4.2 exon 2390053 2390197 2390053 ID=MS.gene04738.t1.exon3;Parent=MS.gene04738.t1
chr4.2 CDS 2390053 2390197 2390053 ID=cds.MS.gene04738.t1;Parent=MS.gene04738.t1
chr4.2 exon 2390368 2390429 2390368 ID=MS.gene04738.t1.exon4;Parent=MS.gene04738.t1
chr4.2 CDS 2390368 2390429 2390368 ID=cds.MS.gene04738.t1;Parent=MS.gene04738.t1
chr4.2 exon 2390516 2390553 2390516 ID=MS.gene04738.t1.exon5;Parent=MS.gene04738.t1
chr4.2 CDS 2390516 2390553 2390516 ID=cds.MS.gene04738.t1;Parent=MS.gene04738.t1
Gene Sequence

>MS.gene04738

ATGTCTGTGCCAGTGGAACAAGGTTATGGAGAGGTTCCTTCAATGGAAACCAAAGAGAGAAGCATTGGTTTGAACCTCAAAGCTACTGAACTGAGGCTAGGGTTACCAGGCTCAGAGTCACCAGAGAGAGAAAATGGTGGTGTGCTTAAGAGTTTGGTGTCTGGTGCCAAAAGGGGTTTCTCTGATGCTATTACTGATGGAGGTTCTGGGAAGTGGGTTTTGTCTGGAAATGGTGGATCTGAAGTGGGGTTGTGTAAAGATGGAAACTTGTTCTCTCCTAAAGCTAAAGGTGGTGGTGAATGTAATAACCAACAAAACCCTTTCTCTGCTTCAGTTGTTGTTAAAGAAACTGTTACACACTCTCCAAAGCCTTTGCATGATAACAAACCACATGTTTCTCCTCCTTCTGCAAAGTAAGATTTCTTCTTATTTGGTTGTATGTTATGTTTATCTTAGTGTTTGTGTATGTTGCTGTGCTTTGTTGATTGATGTTGGCTCTTTGTTCATTGAAATATTTTTTGTTTATTTGTCTTGTCAGTTATCACTATTTAGTGTTTATAGTTTCTTCTGTGAGTTTACAAAATGTTGAAAACAAGAACAAAATTATGTTGAATCTGATGTTTCTTTCTCCCTCTCTAGAAGATCTTCTACATTAATTTGTCAGTTGGAAATTTGAACAGAAGTCAACTAACTCACTTTTTTTTTTACAAGAACAAACTCACTTGTTGATTCGCACTGGTTGGATAAACAACTTAATTAAGTGCTTATAGCATTAGCACTTATCTTATATCTTATAAGTGCTTATATATAGCTATTTCCATAATAAAAGATACAACAAGTTCAAAGTGTTTTCATTTAAGCAATTTAAGCTATGAACTTTTTTCACAAGCTATTATGGAGAGCTTATGCTCATGTCATAAGTTGTATCTATAAGCTCTCCCAAACAGTCTCAAAAGTGATTATACTAGTAGATAAGCTCAAATAAGCCAATCCAAATAGGCCTTGGTGGTTGTTGCTAATTATATTCTTATGTTAGCCTCTGGTGTAAAAGCTGGCGTTGTTACTTGTAGTTGTATGTGCTCTAGTAAATTTCAGGATTTTCTATGTGCTAATCTTCAAAATTTCACATAGATAGTTACTTTTGGTTATTCCTTAAATTTTCTTCTGCATGTTGCATTCAATATCTTAAAAGTTTAAAATTTTATCTTAAAAGTTTTTTCTTGTGCCCTTGTGTATGCCCTGGGATTTGATTTTGTCCAGTTAGCCTTTTCCATGTCTTCTGATGCTGAAAGGTTTTTACTCAGCTTCTGTATTCATTGATTTTCACCAACGCATGATAATATAAATCTGTTTTAGCTTGATTCCTTAGTTTTGAATTTATGATCGTTGGCTTTAAAATTGATTTCTTTTTTCTCAATACGGTTTTTATGCGAATCAGGGCGCAAGTTGTGGGATGGCCTCCAATCCGATCTTTCCGGAAGAACTCAATGGTTTCACAGCCTCAAAAGAATGATGCTGATGCAGAAGCCAAGTCTGAATGCATATATGTAAAAGTCAGCATGGAAGGTGCTCCATACTTGAGGAAAGTGGATCTGAATGGTTTTAGCTCTTATAGGGAACTTTCTTCAGCACTCGAAAAGATGTTCAGTTGTTTTACAATCAGTGAGTTCTTATCTACTTGTTATCGTCTTCTCCCATTATCTGTGTGTCTTGATGTTTTCACCGATGCATGATCACAAATATCTCTCGGTGATGTGATGGTGATCATTCTAGTAAATGTATTGTACTTTGACGAACATTTTAGTTAAATGAGCAAAAATATTTGAAAATCGAGGTGTGTCTTGAATGGTTCAATAAATTCTTTACTAGAAAAAGTTCTTTATATAATTAATTGCATATGAAAAAGAAAACGTAATGAAACCAAATGTTAAACTTAAATAGTTAGCTCAAACACACAGACACACATTTTACCTATACTTTGACAGTACAGTTGTTGATGACATGAATTTATGAACATCGGTCAATGTCAGTGTTGTATAGTGTTTCAATTGAAGAGAACCGTTACCAATACACTCTCTTTCAACCATTTTCTCTATTGTTGGTTGAAATTATGGCATATAAAAACTATTATAGAATATGCATGTATTTATATGTCACAGGTTCTGATTGTGGTGCCAAGTGCCAACATGGTTGAGAATGAAGATTACCTATACAGTAGTCATACCTTTGCATGCCTTTTTTGCTTAAATAAAATGATTCTGATGATGTTTTTGTTGACAAATCCAGGTCAATGTGGCTCATATGGAGTTTCTTGTCGAGAAAAAAATCTAAGTGAAAGTAGATTGGTGGATCTCCTTCATGGTTCAGAATATGTGCTTACCTATGAAGACAAGGATGGTGATTGGATGCTAGTTGGTGATGTTCCATGGGAGTACGTCTATTTTCTTATATATAATTCATAATTCACTACATGAATTAACAATGATTAGCTATGTTTGCTGAATTGATAGTTAGACTTATTTTGGCCTATGAACAATGTTAGAATCAACAAAGAGTGATTTAATTCTGATATCTGAAATGATGTTATTTTTGTTATTTTAGGATGTTCACCGAGTCTTGCAAGAGGTTGAGGATAATGAAGAGTTCTGAAGCAATTGGGCTAGGTAAGACCAATGTTTGTTATATTAAAATCTCATGTATGATACCTTCATATTTTTTGTCGGACTCATGTATACTCTTATTCCTGCAGCACCTCGAGCCATGGAGAAATGTAAAAGTCGCAACTAG
Protein sequence

>MS.gene04738.t1

MSVPVEQGYGEVPSMETKERSIGLNLKATELRLGLPGSESPERENGGVLKSLVSGAKRGFSDAITDGGSGKWVLSGNGGSEVGLCKDGNLFSPKAKGGGECNNQQNPFSASVVVKETVTHSPKPLHDNKPHVSPPSAKAQVVGWPPIRSFRKNSMVSQPQKNDADAEAKSECIYVKVSMEGAPYLRKVDLNGFSSYRELSSALEKMFSCFTISQCGSYGVSCREKNLSESRLVDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCKRLRIMKSSEAIGLAPRAMEKCKSRN