Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048346.t1 | KEH20135.1 | 90.5 | 953 | 91 | 0 | 1 | 953 | 33 | 985 | 0.00E+00 | 1290 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048346.t1 | F4IJK1 | 39.9 | 985 | 490 | 17 | 1 | 956 | 33 | 944 | 1.2e-87 | 326.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048346.t1 | A0A072U2T5 | 90.5 | 953 | 91 | 0 | 1 | 953 | 33 | 985 | 0.0e+00 | 1290.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene048346 | MS.gene010090 | PPI |
| MS.gene048346 | MS.gene21314 | PPI |
| MS.gene048346 | MS.gene37922 | PPI |
| MS.gene049982 | MS.gene048346 | PPI |
| MS.gene013085 | MS.gene048346 | PPI |
| MS.gene020439 | MS.gene048346 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048346.t1 | MTR_8g471130 | 95.912 | 954 | 39 | 0 | 1 | 954 | 33 | 986 | 0.0 | 1846 |
| MS.gene048346.t1 | MTR_5g032060 | 51.642 | 1127 | 357 | 21 | 1 | 954 | 33 | 1144 | 0.0 | 970 |
| MS.gene048346.t1 | MTR_3g100190 | 43.249 | 511 | 255 | 12 | 1 | 496 | 30 | 520 | 1.47e-107 | 356 |
| MS.gene048346.t1 | MTR_5g066340 | 39.410 | 373 | 165 | 10 | 634 | 947 | 590 | 960 | 1.87e-65 | 238 |
| MS.gene048346.t1 | MTR_1g071540 | 50.000 | 94 | 41 | 1 | 1 | 88 | 38 | 131 | 5.45e-23 | 106 |
| MS.gene048346.t1 | MTR_1g103160 | 47.000 | 100 | 50 | 1 | 1 | 97 | 1 | 100 | 8.83e-23 | 105 |
| MS.gene048346.t1 | MTR_1g103160 | 47.000 | 100 | 50 | 1 | 1 | 97 | 36 | 135 | 9.05e-23 | 105 |
| MS.gene048346.t1 | MTR_1g103160 | 47.000 | 100 | 50 | 1 | 1 | 97 | 33 | 132 | 1.03e-22 | 105 |
| MS.gene048346.t1 | MTR_5g006520 | 28.652 | 356 | 182 | 9 | 4 | 330 | 39 | 351 | 1.41e-22 | 105 |
| MS.gene048346.t1 | MTR_5g006520 | 28.652 | 356 | 182 | 9 | 4 | 330 | 39 | 351 | 2.34e-22 | 104 |
| MS.gene048346.t1 | MTR_6g016950 | 81.481 | 54 | 10 | 0 | 731 | 784 | 7 | 60 | 5.66e-22 | 90.5 |
| MS.gene048346.t1 | MTR_7g095410 | 44.340 | 106 | 48 | 1 | 1 | 106 | 1 | 95 | 2.66e-21 | 101 |
| MS.gene048346.t1 | MTR_7g095410 | 44.340 | 106 | 48 | 1 | 1 | 106 | 36 | 130 | 2.73e-21 | 101 |
| MS.gene048346.t1 | MTR_7g095410 | 44.340 | 106 | 48 | 1 | 1 | 106 | 36 | 130 | 2.78e-21 | 101 |
| MS.gene048346.t1 | MTR_5g075490 | 45.455 | 99 | 44 | 3 | 1 | 99 | 44 | 132 | 2.17e-18 | 90.5 |
| MS.gene048346.t1 | MTR_3g467610 | 47.312 | 93 | 44 | 2 | 1 | 91 | 15 | 104 | 7.43e-18 | 88.6 |
| MS.gene048346.t1 | MTR_7g108070 | 50.746 | 67 | 33 | 0 | 1 | 67 | 35 | 101 | 3.33e-16 | 80.9 |
| MS.gene048346.t1 | MTR_1g088965 | 39.759 | 83 | 46 | 1 | 1 | 79 | 39 | 121 | 1.37e-12 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048346.t1 | AT5G10500 | 44.570 | 442 | 213 | 7 | 1 | 437 | 33 | 447 | 1.20e-104 | 345 |
| MS.gene048346.t1 | AT5G10500 | 42.994 | 314 | 141 | 6 | 634 | 947 | 558 | 833 | 3.60e-56 | 209 |
| MS.gene048346.t1 | AT2G22560 | 54.573 | 328 | 125 | 7 | 634 | 956 | 636 | 944 | 5.40e-96 | 324 |
| MS.gene048346.t1 | AT1G09720 | 41.892 | 444 | 221 | 7 | 1 | 436 | 33 | 447 | 7.10e-91 | 310 |
| MS.gene048346.t1 | AT1G09720 | 40.270 | 370 | 169 | 10 | 626 | 948 | 537 | 901 | 6.95e-66 | 239 |
| MS.gene048346.t1 | AT1G58215 | 39.609 | 409 | 182 | 8 | 605 | 948 | 514 | 922 | 1.96e-68 | 247 |
| MS.gene048346.t1 | AT3G22790 | 28.692 | 474 | 260 | 16 | 1 | 435 | 35 | 469 | 1.73e-30 | 130 |
| MS.gene048346.t1 | AT3G22790 | 28.692 | 474 | 260 | 16 | 1 | 435 | 35 | 469 | 1.73e-30 | 130 |
| MS.gene048346.t1 | AT3G22790 | 28.692 | 474 | 260 | 16 | 1 | 435 | 35 | 469 | 1.73e-30 | 130 |
| MS.gene048346.t1 | AT4G14760 | 27.719 | 469 | 270 | 18 | 1 | 435 | 35 | 468 | 5.13e-24 | 110 |
| MS.gene048346.t1 | AT4G14760 | 27.719 | 469 | 270 | 18 | 1 | 435 | 35 | 468 | 5.13e-24 | 110 |
| MS.gene048346.t1 | AT4G14760 | 27.719 | 469 | 270 | 18 | 1 | 435 | 35 | 468 | 5.13e-24 | 110 |
| MS.gene048346.t1 | AT4G14760 | 27.719 | 469 | 270 | 18 | 1 | 435 | 35 | 468 | 5.13e-24 | 110 |
| MS.gene048346.t1 | AT1G03080 | 34.868 | 152 | 88 | 2 | 1 | 146 | 35 | 181 | 1.14e-21 | 102 |
| MS.gene048346.t1 | AT1G03080 | 34.868 | 152 | 88 | 2 | 1 | 146 | 35 | 181 | 1.14e-21 | 102 |
| MS.gene048346.t1 | AT1G03080 | 34.868 | 152 | 88 | 2 | 1 | 146 | 35 | 181 | 1.14e-21 | 102 |
| MS.gene048346.t1 | AT4G02710 | 30.000 | 280 | 164 | 9 | 1 | 257 | 36 | 306 | 5.73e-21 | 99.8 |
| MS.gene048346.t1 | AT2G30500 | 57.812 | 64 | 26 | 1 | 1 | 64 | 44 | 106 | 4.41e-16 | 82.8 |
| MS.gene048346.t1 | AT2G30500 | 57.812 | 64 | 26 | 1 | 1 | 64 | 44 | 106 | 4.41e-16 | 82.8 |
| MS.gene048346.t1 | AT2G30500 | 57.812 | 64 | 26 | 1 | 1 | 64 | 43 | 105 | 4.59e-16 | 82.8 |
| MS.gene048346.t1 | AT2G47920 | 42.391 | 92 | 46 | 1 | 1 | 85 | 32 | 123 | 7.46e-15 | 75.5 |
| MS.gene048346.t1 | AT2G47920 | 42.391 | 92 | 46 | 1 | 1 | 85 | 32 | 123 | 7.46e-15 | 75.5 |
| MS.gene048346.t1 | AT1G03470 | 59.259 | 54 | 22 | 0 | 1 | 54 | 30 | 83 | 1.71e-14 | 75.5 |
| MS.gene048346.t1 | AT1G03470 | 59.259 | 54 | 22 | 0 | 1 | 54 | 30 | 83 | 1.71e-14 | 75.5 |
| MS.gene048346.t1 | AT1G03470 | 59.259 | 54 | 22 | 0 | 1 | 54 | 30 | 83 | 1.71e-14 | 75.5 |
| MS.gene048346.t1 | AT1G03470 | 59.259 | 54 | 22 | 0 | 1 | 54 | 30 | 83 | 1.71e-14 | 75.5 |
| MS.gene048346.t1 | AT5G58320 | 51.613 | 62 | 30 | 0 | 1 | 62 | 1 | 62 | 1.14e-13 | 75.1 |
| MS.gene048346.t1 | AT5G58320 | 51.613 | 62 | 30 | 0 | 1 | 62 | 44 | 105 | 1.97e-13 | 74.7 |
| MS.gene048346.t1 | AT5G58320 | 51.613 | 62 | 30 | 0 | 1 | 62 | 44 | 105 | 1.97e-13 | 74.7 |
| MS.gene048346.t1 | AT5G58320 | 51.613 | 62 | 30 | 0 | 1 | 62 | 44 | 105 | 1.98e-13 | 74.7 |
| MS.gene048346.t1 | AT5G58320 | 51.613 | 62 | 30 | 0 | 1 | 62 | 44 | 105 | 1.98e-13 | 74.7 |
Find 126 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATCATTCAATAAGATTT+TGG | 0.176661 | 8.3:-34397187 | None:intergenic |
| ATGCTGTTGTTATCTCTTCT+TGG | 0.181149 | 8.3:-34398091 | None:intergenic |
| TTTCTACTCAATCCTGATTT+AGG | 0.182892 | 8.3:-34395986 | None:intergenic |
| CCTTACTTCCCTTACTCTTT+TGG | 0.192513 | 8.3:-34396314 | None:intergenic |
| CCTCATTCGGTATCACCTTT+TGG | 0.233093 | 8.3:-34397068 | None:intergenic |
| AATCCTGATTTAGGAGCTTT+AGG | 0.233147 | 8.3:-34395977 | None:intergenic |
| ATAATTCCATTGCTAGATAT+TGG | 0.234388 | 8.3:+34396092 | MS.gene048346:CDS |
| ACCGAGTTCGTCTTGCAAAT+TGG | 0.261286 | 8.3:-34396155 | None:intergenic |
| TCAACAGCAGAAGTTGATTC+TGG | 0.268774 | 8.3:-34397756 | None:intergenic |
| CCTCGTCTGGTATCACCTTT+TGG | 0.271862 | 8.3:-34396861 | None:intergenic |
| CCTCTAAGGTCATTTATCTT+TGG | 0.277004 | 8.3:+34398359 | MS.gene048346:CDS |
| TTTACATCTCGTTTCGGCCT+CGG | 0.284477 | 8.3:-34396394 | None:intergenic |
| GCAAACGTGACAATGCTTCT+TGG | 0.304395 | 8.3:-34396249 | None:intergenic |
| TCCATATAAGGAACTCTATC+TGG | 0.306812 | 8.3:-34395785 | None:intergenic |
| AAATCTTTGAGTGGAGTCTT+TGG | 0.314691 | 8.3:-34395884 | None:intergenic |
| CTGCTGTTGAAGAGAAGTTT+AGG | 0.318730 | 8.3:+34397769 | MS.gene048346:CDS |
| ACGTTTGGTTTCAATCCTTC+TGG | 0.318970 | 8.3:-34395851 | None:intergenic |
| CAATACGTCCATTTATTCAT+TGG | 0.319468 | 8.3:+34397218 | MS.gene048346:CDS |
| CAATACGGCCATTGATTCAT+TGG | 0.327975 | 8.3:+34397035 | MS.gene048346:CDS |
| AGTGGAGTCTTTGGAGCTTT+CGG | 0.333188 | 8.3:-34395875 | None:intergenic |
| CAATGAATCAATGGCCGTAT+TGG | 0.335865 | 8.3:-34397034 | None:intergenic |
| TGAAACGGAAAAGGAAAGTT+TGG | 0.339852 | 8.3:+34396681 | MS.gene048346:CDS |
| TCCAGATAGAGTTCCTTATA+TGG | 0.346090 | 8.3:+34395784 | MS.gene048346:CDS |
| TAGAGGAGAATCTAGAATTC+TGG | 0.350291 | 8.3:+34397811 | MS.gene048346:CDS |
| AAATCATGAAAAGGATTATA+AGG | 0.353380 | 8.3:+34397380 | MS.gene048346:CDS |
| TAATTCCATTGCTAGATATT+GGG | 0.353589 | 8.3:+34396093 | MS.gene048346:CDS |
| TAAAGTTGCTGATGAGCTTC+AGG | 0.359570 | 8.3:+34398208 | MS.gene048346:CDS |
| TCTCTTTCTTTCAACACTAT+AGG | 0.361479 | 8.3:-34397306 | None:intergenic |
| TCGTCGTCCTCATCCATATA+AGG | 0.365262 | 8.3:-34395797 | None:intergenic |
| TGTGATTTAACATCATGTTT+TGG | 0.365811 | 8.3:-34396961 | None:intergenic |
| GTCACGTTTGCAAGAGAAAC+AGG | 0.366657 | 8.3:+34396261 | MS.gene048346:CDS |
| CATATTGAAGTTGATTCATA+AGG | 0.369783 | 8.3:-34396351 | None:intergenic |
| TATCGAAGCTCGAAACAAAA+TGG | 0.379973 | 8.3:+34397904 | MS.gene048346:CDS |
| TCAACAAAGTTTATTAGCTC+TGG | 0.383758 | 8.3:-34395668 | None:intergenic |
| CCAAAGATAAATGACCTTAG+AGG | 0.389815 | 8.3:-34398359 | None:intergenic |
| CCAAAAGAGTAAGGGAAGTA+AGG | 0.391738 | 8.3:+34396314 | MS.gene048346:CDS |
| ACTCTTTGAGTCATCATTGC+AGG | 0.395563 | 8.3:+34397563 | MS.gene048346:CDS |
| TTTCGAGGCTGGCTCAAATT+CGG | 0.396521 | 8.3:+34396513 | MS.gene048346:CDS |
| GACAGAAGCAATTGTGTTGT+TGG | 0.400635 | 8.3:-34395759 | None:intergenic |
| TGTGATTTAACGTTATGTTT+CGG | 0.407395 | 8.3:-34397147 | None:intergenic |
| CGAAGAACCGTGTTCCTCTA+AGG | 0.410206 | 8.3:+34398345 | MS.gene048346:CDS |
| CAATGAATAAATGGACGTAT+TGG | 0.417435 | 8.3:-34397217 | None:intergenic |
| TTTGGAGCTTTCGGAACGTT+TGG | 0.418385 | 8.3:-34395866 | None:intergenic |
| TCGAATCCGAAGGTCTCATC+TGG | 0.419762 | 8.3:-34398325 | None:intergenic |
| AGTTTCTTCTCAAACAGCTT+TGG | 0.420468 | 8.3:+34396606 | MS.gene048346:CDS |
| TTCAAAATGAACTCACACTA+TGG | 0.420988 | 8.3:+34398006 | MS.gene048346:CDS |
| TACAAAAGATTTACTGATTG+AGG | 0.424969 | 8.3:+34397881 | MS.gene048346:CDS |
| GAACGAGAAACTTAGAGAAA+TGG | 0.426500 | 8.3:+34396723 | MS.gene048346:CDS |
| TACTGTTGTTGCTTTCTCCT+CGG | 0.428816 | 8.3:-34397442 | None:intergenic |
| GGCTTTAACCGTGACAGAAA+TGG | 0.430771 | 8.3:+34396534 | MS.gene048346:CDS |
| ACTTAGAGAAATGGACGAAA+TGG | 0.431565 | 8.3:+34396732 | MS.gene048346:CDS |
| AAATCGAGTCCAAAAGAGTA+AGG | 0.433279 | 8.3:+34396305 | MS.gene048346:CDS |
| TTTAAAGCTTCTAGAAGAAG+AGG | 0.435154 | 8.3:+34395601 | MS.gene048346:CDS |
| GATACCAGACGAGGACGATA+TGG | 0.437799 | 8.3:+34396870 | MS.gene048346:CDS |
| ACTCCTAAAGCTCCTAAATC+AGG | 0.441379 | 8.3:+34395974 | MS.gene048346:CDS |
| TCGAATCTCTTCGTCTTTCA+CGG | 0.443211 | 8.3:-34397610 | None:intergenic |
| CGAGATGTAAAAGAAGTAAC+AGG | 0.444047 | 8.3:+34396406 | MS.gene048346:CDS |
| CTCACCTTATCCTCCTCATT+CGG | 0.449905 | 8.3:-34397081 | None:intergenic |
| TGTTTCATATTTCTGTATCT+CGG | 0.450311 | 8.3:-34397856 | None:intergenic |
| GAAGATTAAAGAACATTTCG+AGG | 0.454058 | 8.3:+34396498 | MS.gene048346:CDS |
| GAGCAGAAATGTATTACAAA+AGG | 0.465180 | 8.3:+34395642 | MS.gene048346:CDS |
| AAATGAACTCACACTATGGC+TGG | 0.467020 | 8.3:+34398010 | MS.gene048346:CDS |
| CGAGGAGGCTCGCCGTTTGA+TGG | 0.468797 | 8.3:+34396204 | MS.gene048346:CDS |
| ATTAAAGAACATTTCGAGGC+TGG | 0.469351 | 8.3:+34396502 | MS.gene048346:CDS |
| GTTGCTGATGAGCTTCAGGC+TGG | 0.477201 | 8.3:+34398212 | MS.gene048346:CDS |
| GCTGGTTCGCAACCTTGAAA+CGG | 0.479618 | 8.3:+34396666 | MS.gene048346:CDS |
| TTGGAGGGAAATGAAGAAAT+CGG | 0.485167 | 8.3:+34397669 | MS.gene048346:CDS |
| TGTTCTGATCTTCAACAACT+TGG | 0.492297 | 8.3:-34396777 | None:intergenic |
| ACCAGAAAATCATGTTATTG+AGG | 0.492750 | 8.3:+34397713 | MS.gene048346:CDS |
| ATTGGAAAATGAAGTCCAAA+AGG | 0.496529 | 8.3:+34397053 | MS.gene048346:CDS |
| TCTCTCTGATAAAGTCCAAA+AGG | 0.496569 | 8.3:+34396846 | MS.gene048346:CDS |
| ATAATACAATCAATTGATGC+TGG | 0.499961 | 8.3:+34397357 | MS.gene048346:CDS |
| ATATTGAAGTTGATTCATAA+GGG | 0.500924 | 8.3:-34396350 | None:intergenic |
| GGAAACTGAGGGACAGATCA+AGG | 0.501428 | 8.3:+34396114 | MS.gene048346:CDS |
| CTCACCATATCGTCCTCGTC+TGG | 0.501832 | 8.3:-34396874 | None:intergenic |
| TCATATTCAAAACCTCGCCT+TGG | 0.503348 | 8.3:-34398172 | None:intergenic |
| GAGAAACCAGATGAGACCTT+CGG | 0.505800 | 8.3:+34398319 | MS.gene048346:CDS |
| CAAAAGAGTAAGGGAAGTAA+GGG | 0.506192 | 8.3:+34396315 | MS.gene048346:CDS |
| TAAAGTATTTGAAACAGAAG+AGG | 0.511073 | 8.3:+34396897 | MS.gene048346:CDS |
| GATACCGAATGAGGAGGATA+AGG | 0.512984 | 8.3:+34397077 | MS.gene048346:CDS |
| TGATGCTGGAAATCATGAAA+AGG | 0.514888 | 8.3:+34397371 | MS.gene048346:CDS |
| TCGCAACCTTGAAACGGAAA+AGG | 0.515404 | 8.3:+34396672 | MS.gene048346:CDS |
| TCCAATTTGCAAGACGAACT+CGG | 0.522122 | 8.3:+34396154 | MS.gene048346:CDS |
| ATCAAGACTACAATGCGCTT+CGG | 0.526939 | 8.3:-34396821 | None:intergenic |
| CGTACCTATCGGCTAAAGAT+CGG | 0.533688 | 8.3:-34395709 | None:intergenic |
| TATAATGCTGCGAAATTCCA+AGG | 0.542876 | 8.3:+34398155 | MS.gene048346:CDS |
| AACTGATGAACTCCATTCGC+TGG | 0.544339 | 8.3:+34396648 | MS.gene048346:CDS |
| GAGCCGCTTCTGCCATCAAA+CGG | 0.544838 | 8.3:-34396216 | None:intergenic |
| TTAAAGCTTCTAGAAGAAGA+GGG | 0.544929 | 8.3:+34395602 | MS.gene048346:CDS |
| AAAGGATTTGAGTGAAGATG+TGG | 0.556266 | 8.3:+34396432 | MS.gene048346:CDS |
| TAGAGTTCCTTATATGGATG+AGG | 0.558198 | 8.3:+34395790 | MS.gene048346:CDS |
| TTGCAAGACGAACTCGGTGA+AGG | 0.558534 | 8.3:+34396160 | MS.gene048346:CDS |
| TCTTCAACTAGATGCTGAGA+AGG | 0.561168 | 8.3:+34398253 | MS.gene048346:CDS |
| TTGCTGATGAGCTTCAGGCT+GGG | 0.562771 | 8.3:+34398213 | MS.gene048346:CDS |
| TGTTGAAGAGAAGTTTAGGA+TGG | 0.566244 | 8.3:+34397773 | MS.gene048346:CDS |
| TCTGCAAGGTGCTTATAAAG+TGG | 0.568092 | 8.3:-34397981 | None:intergenic |
| TGCTAGATATTGGGAAACTG+AGG | 0.569587 | 8.3:+34396102 | MS.gene048346:CDS |
| GGAAAACAGCGCGTTGCTGA+AGG | 0.570843 | 8.3:+34398031 | MS.gene048346:CDS |
| TCGCCGTTTGATGGCAGAAG+CGG | 0.572967 | 8.3:+34396213 | MS.gene048346:CDS |
| TGATGATGCAAAATCCAATA+CGG | 0.576914 | 8.3:+34397020 | MS.gene048346:CDS |
| TAGAAAGCAAGCAATTGAAG+AGG | 0.578147 | 8.3:+34396003 | MS.gene048346:CDS |
| CAAGGTTGCGAACCAGCGAA+TGG | 0.582849 | 8.3:-34396660 | None:intergenic |
| GTTGAAGAGAAGTTTAGGAT+GGG | 0.590016 | 8.3:+34397774 | MS.gene048346:CDS |
| CCAAAAGGTGATACCGAATG+AGG | 0.590400 | 8.3:+34397068 | MS.gene048346:CDS |
| TTATAAGGAAATTTCATCTG+AGG | 0.593385 | 8.3:+34397395 | MS.gene048346:CDS |
| CTTGTTACTGAATTTCCTTG+TGG | 0.598228 | 8.3:-34395933 | None:intergenic |
| TATATCATCATTTCAAAGCA+CGG | 0.601898 | 8.3:-34398433 | None:intergenic |
| ACAAAATGGAAAGCAACAGA+AGG | 0.603333 | 8.3:+34397918 | MS.gene048346:CDS |
| TGCTGCGAAATTCCAAGGCG+AGG | 0.604091 | 8.3:+34398160 | MS.gene048346:CDS |
| AAAAGAAGTAACAGGAACAA+AGG | 0.607695 | 8.3:+34396414 | MS.gene048346:CDS |
| TGATAAGTGATCGTACCTAT+CGG | 0.609920 | 8.3:-34395720 | None:intergenic |
| ACGACGACGAAGCATCGCCA+AGG | 0.611893 | 8.3:+34395813 | MS.gene048346:CDS |
| AATCGAGTCCAAAAGAGTAA+GGG | 0.614608 | 8.3:+34396306 | MS.gene048346:CDS |
| GGGAATCGACGAACTTCTAG+AGG | 0.619415 | 8.3:+34397794 | MS.gene048346:CDS |
| AGTTTCCCAATATCTAGCAA+TGG | 0.619821 | 8.3:-34396098 | None:intergenic |
| TGTTAATGTTATTGATGACG+AGG | 0.631377 | 8.3:+34396186 | MS.gene048346:CDS |
| CGGCTAAAGATCGGTATGCA+CGG | 0.631655 | 8.3:-34395700 | None:intergenic |
| AATCCAATTCGCAAAAGCCG+AGG | 0.632575 | 8.3:+34396377 | MS.gene048346:CDS |
| ACACGGTTCTTCGAATCCGA+AGG | 0.634630 | 8.3:-34398335 | None:intergenic |
| GCTAGATATTGGGAAACTGA+GGG | 0.645079 | 8.3:+34396103 | MS.gene048346:CDS |
| TAATGTTATTGATGACGAGG+AGG | 0.647964 | 8.3:+34396189 | MS.gene048346:CDS |
| CATACCGATCTTTAGCCGAT+AGG | 0.649267 | 8.3:+34395705 | MS.gene048346:CDS |
| AAAGGTGATACCGAATGAGG+AGG | 0.672192 | 8.3:+34397071 | MS.gene048346:CDS |
| TAACGACAGCGACAGCCACA+AGG | 0.673857 | 8.3:+34395918 | MS.gene048346:CDS |
| CCAAAAGGTGATACCAGACG+AGG | 0.715557 | 8.3:+34396861 | MS.gene048346:CDS |
| TAAATGACCTTAGAGGAACA+CGG | 0.755952 | 8.3:-34398352 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCATGAAAAGGATTATA+AGG | + | chr8.3:34397380-34397399 | MS.gene048346:CDS | 20.0% |
| !!! | ATATTGAAGTTGATTCATAA+GGG | - | chr8.3:34396353-34396372 | None:intergenic | 20.0% |
| !!! | ATCATCATTCAATAAGATTT+TGG | - | chr8.3:34397190-34397209 | None:intergenic | 20.0% |
| !!! | TTCATTTTCCAATGAATAAA+TGG | - | chr8.3:34397229-34397248 | None:intergenic | 20.0% |
| ! | ATAATACAATCAATTGATGC+TGG | + | chr8.3:34397357-34397376 | MS.gene048346:CDS | 25.0% |
| ! | ATAATTCCATTGCTAGATAT+TGG | + | chr8.3:34396092-34396111 | MS.gene048346:CDS | 25.0% |
| ! | GCAAAGTTGAATGAAAAATT+TGG | + | chr8.3:34398281-34398300 | MS.gene048346:CDS | 25.0% |
| ! | TAATTCCATTGCTAGATATT+GGG | + | chr8.3:34396093-34396112 | MS.gene048346:CDS | 25.0% |
| ! | TGTGATTTAACGTTATGTTT+CGG | - | chr8.3:34397150-34397169 | None:intergenic | 25.0% |
| ! | TTATAAGGAAATTTCATCTG+AGG | + | chr8.3:34397395-34397414 | MS.gene048346:CDS | 25.0% |
| !! | CATATTGAAGTTGATTCATA+AGG | - | chr8.3:34396354-34396373 | None:intergenic | 25.0% |
| !! | TAAAGTATTTGAAACAGAAG+AGG | + | chr8.3:34396897-34396916 | MS.gene048346:CDS | 25.0% |
| !! | TACAAAAGATTTACTGATTG+AGG | + | chr8.3:34397881-34397900 | MS.gene048346:CDS | 25.0% |
| !! | TGTGATTTAACATCATGTTT+TGG | - | chr8.3:34396964-34396983 | None:intergenic | 25.0% |
| !! | TGTTTCATATTTCTGTATCT+CGG | - | chr8.3:34397859-34397878 | None:intergenic | 25.0% |
| !! | TTCATTTTCCAATGAATCAA+TGG | - | chr8.3:34397046-34397065 | None:intergenic | 25.0% |
| !!! | ACACTTTTTAAATCTTTGAG+TGG | - | chr8.3:34395896-34395915 | None:intergenic | 25.0% |
| !!! | ATAGACTGTTTTTGTTTCTT+TGG | - | chr8.3:34398392-34398411 | None:intergenic | 25.0% |
| !!! | TTCTTCTTTTGTTTGTGAAT+CGG | - | chr8.3:34397262-34397281 | None:intergenic | 25.0% |
| AAAAGAAGTAACAGGAACAA+AGG | + | chr8.3:34396414-34396433 | MS.gene048346:CDS | 30.0% | |
| ACCAGAAAATCATGTTATTG+AGG | + | chr8.3:34397713-34397732 | MS.gene048346:CDS | 30.0% | |
| ATTGGAAAATGAAGTCCAAA+AGG | + | chr8.3:34397053-34397072 | MS.gene048346:CDS | 30.0% | |
| CAATACGTCCATTTATTCAT+TGG | + | chr8.3:34397218-34397237 | MS.gene048346:CDS | 30.0% | |
| CAATGAATAAATGGACGTAT+TGG | - | chr8.3:34397220-34397239 | None:intergenic | 30.0% | |
| GAAGATTAAAGAACATTTCG+AGG | + | chr8.3:34396498-34396517 | MS.gene048346:CDS | 30.0% | |
| GAGCAGAAATGTATTACAAA+AGG | + | chr8.3:34395642-34395661 | MS.gene048346:CDS | 30.0% | |
| TCAACAAAGTTTATTAGCTC+TGG | - | chr8.3:34395671-34395690 | None:intergenic | 30.0% | |
| TCTCTTTCTTTCAACACTAT+AGG | - | chr8.3:34397309-34397328 | None:intergenic | 30.0% | |
| TTCAAAATGAACTCACACTA+TGG | + | chr8.3:34398006-34398025 | MS.gene048346:CDS | 30.0% | |
| TTTCTACTCAATCCTGATTT+AGG | - | chr8.3:34395989-34396008 | None:intergenic | 30.0% | |
| ! | ACTTCTTTTACATCTCGTTT+CGG | - | chr8.3:34396403-34396422 | None:intergenic | 30.0% |
| ! | AGTCTATCTTTTCAATGCAT+AGG | + | chr8.3:34398405-34398424 | MS.gene048346:CDS | 30.0% |
| ! | TCAATAACATGATTTTCTGG+TGG | - | chr8.3:34397714-34397733 | None:intergenic | 30.0% |
| ! | TCCTCAATAACATGATTTTC+TGG | - | chr8.3:34397717-34397736 | None:intergenic | 30.0% |
| ! | TGATGATGCAAAATCCAATA+CGG | + | chr8.3:34397020-34397039 | MS.gene048346:CDS | 30.0% |
| ! | TTAAAGCTTCTAGAAGAAGA+GGG | + | chr8.3:34395602-34395621 | MS.gene048346:CDS | 30.0% |
| ! | TTTAAAGCTTCTAGAAGAAG+AGG | + | chr8.3:34395601-34395620 | MS.gene048346:CDS | 30.0% |
| !! | TACTTTTAAAGAAGATTCCG+AGG | + | chr8.3:34397425-34397444 | MS.gene048346:CDS | 30.0% |
| !! | TGTTAATGTTATTGATGACG+AGG | + | chr8.3:34396186-34396205 | MS.gene048346:CDS | 30.0% |
| !!! | ATGCTTTGCTTTTAGAGTAT+AGG | + | chr8.3:34397472-34397491 | MS.gene048346:CDS | 30.0% |
| AAAGGATTTGAGTGAAGATG+TGG | + | chr8.3:34396432-34396451 | MS.gene048346:CDS | 35.0% | |
| AAATCGAGTCCAAAAGAGTA+AGG | + | chr8.3:34396305-34396324 | MS.gene048346:CDS | 35.0% | |
| AAATCTTTGAGTGGAGTCTT+TGG | - | chr8.3:34395887-34395906 | None:intergenic | 35.0% | |
| AATCCTGATTTAGGAGCTTT+AGG | - | chr8.3:34395980-34395999 | None:intergenic | 35.0% | |
| AATCGAGTCCAAAAGAGTAA+GGG | + | chr8.3:34396306-34396325 | MS.gene048346:CDS | 35.0% | |
| ACAAAATGGAAAGCAACAGA+AGG | + | chr8.3:34397918-34397937 | MS.gene048346:CDS | 35.0% | |
| ACTTAGAGAAATGGACGAAA+TGG | + | chr8.3:34396732-34396751 | MS.gene048346:CDS | 35.0% | |
| AGTTTCCCAATATCTAGCAA+TGG | - | chr8.3:34396101-34396120 | None:intergenic | 35.0% | |
| AGTTTCTTCTCAAACAGCTT+TGG | + | chr8.3:34396606-34396625 | MS.gene048346:CDS | 35.0% | |
| ATGCTGTTGTTATCTCTTCT+TGG | - | chr8.3:34398094-34398113 | None:intergenic | 35.0% | |
| ATTAAAGAACATTTCGAGGC+TGG | + | chr8.3:34396502-34396521 | MS.gene048346:CDS | 35.0% | |
| CAAAAGAGTAAGGGAAGTAA+GGG | + | chr8.3:34396315-34396334 | MS.gene048346:CDS | 35.0% | |
| CCAAAGATAAATGACCTTAG+AGG | - | chr8.3:34398362-34398381 | None:intergenic | 35.0% | |
| CGAGATGTAAAAGAAGTAAC+AGG | + | chr8.3:34396406-34396425 | MS.gene048346:CDS | 35.0% | |
| CTTGTTACTGAATTTCCTTG+TGG | - | chr8.3:34395936-34395955 | None:intergenic | 35.0% | |
| GAACGAGAAACTTAGAGAAA+TGG | + | chr8.3:34396723-34396742 | MS.gene048346:CDS | 35.0% | |
| TAAATGACCTTAGAGGAACA+CGG | - | chr8.3:34398355-34398374 | None:intergenic | 35.0% | |
| TAGAAAGCAAGCAATTGAAG+AGG | + | chr8.3:34396003-34396022 | MS.gene048346:CDS | 35.0% | |
| TAGAGTTCCTTATATGGATG+AGG | + | chr8.3:34395790-34395809 | MS.gene048346:CDS | 35.0% | |
| TATAATGCTGCGAAATTCCA+AGG | + | chr8.3:34398155-34398174 | MS.gene048346:CDS | 35.0% | |
| TATCGAAGCTCGAAACAAAA+TGG | + | chr8.3:34397904-34397923 | MS.gene048346:CDS | 35.0% | |
| TCCAGATAGAGTTCCTTATA+TGG | + | chr8.3:34395784-34395803 | MS.gene048346:CDS | 35.0% | |
| TCCATATAAGGAACTCTATC+TGG | - | chr8.3:34395788-34395807 | None:intergenic | 35.0% | |
| TCTCTCTGATAAAGTCCAAA+AGG | + | chr8.3:34396846-34396865 | MS.gene048346:CDS | 35.0% | |
| TGAAACGGAAAAGGAAAGTT+TGG | + | chr8.3:34396681-34396700 | MS.gene048346:CDS | 35.0% | |
| TGATAAGTGATCGTACCTAT+CGG | - | chr8.3:34395723-34395742 | None:intergenic | 35.0% | |
| TGATGCTGGAAATCATGAAA+AGG | + | chr8.3:34397371-34397390 | MS.gene048346:CDS | 35.0% | |
| TGTTCTGATCTTCAACAACT+TGG | - | chr8.3:34396780-34396799 | None:intergenic | 35.0% | |
| TGTTGAAGAGAAGTTTAGGA+TGG | + | chr8.3:34397773-34397792 | MS.gene048346:CDS | 35.0% | |
| TTGGAGGGAAATGAAGAAAT+CGG | + | chr8.3:34397669-34397688 | MS.gene048346:CDS | 35.0% | |
| ! | AACTTTCCTTTTCCGTTTCA+AGG | - | chr8.3:34396681-34396700 | None:intergenic | 35.0% |
| ! | AAGTCTTTTGCAGAAAAGCT+TGG | + | chr8.3:34397650-34397669 | MS.gene048346:CDS | 35.0% |
| ! | AATCCTTCTGGCATTTTTCT+TGG | - | chr8.3:34395842-34395861 | None:intergenic | 35.0% |
| ! | GTTCATTTTGAATCTCTGCA+AGG | - | chr8.3:34397998-34398017 | None:intergenic | 35.0% |
| ! | GTTGAAGAGAAGTTTAGGAT+GGG | + | chr8.3:34397774-34397793 | MS.gene048346:CDS | 35.0% |
| ! | TAGAGGAGAATCTAGAATTC+TGG | + | chr8.3:34397811-34397830 | MS.gene048346:CDS | 35.0% |
| !! | CCTCTAAGGTCATTTATCTT+TGG | + | chr8.3:34398359-34398378 | MS.gene048346:CDS | 35.0% |
| !! | TAATGTTATTGATGACGAGG+AGG | + | chr8.3:34396189-34396208 | MS.gene048346:CDS | 35.0% |
| !! | TTTTTCTGCCATTTCTGTCA+CGG | - | chr8.3:34396545-34396564 | None:intergenic | 35.0% |
| AAATGAACTCACACTATGGC+TGG | + | chr8.3:34398010-34398029 | MS.gene048346:CDS | 40.0% | |
| ACTCCTAAAGCTCCTAAATC+AGG | + | chr8.3:34395974-34395993 | MS.gene048346:CDS | 40.0% | |
| ACTCTTTGAGTCATCATTGC+AGG | + | chr8.3:34397563-34397582 | MS.gene048346:CDS | 40.0% | |
| ATCAAGACTACAATGCGCTT+CGG | - | chr8.3:34396824-34396843 | None:intergenic | 40.0% | |
| CAATGAATCAATGGCCGTAT+TGG | - | chr8.3:34397037-34397056 | None:intergenic | 40.0% | |
| CCAAAAGAGTAAGGGAAGTA+AGG | + | chr8.3:34396314-34396333 | MS.gene048346:CDS | 40.0% | |
| CTGCTGTTGAAGAGAAGTTT+AGG | + | chr8.3:34397769-34397788 | MS.gene048346:CDS | 40.0% | |
| GACAGAAGCAATTGTGTTGT+TGG | - | chr8.3:34395762-34395781 | None:intergenic | 40.0% | |
| GCTAGATATTGGGAAACTGA+GGG | + | chr8.3:34396103-34396122 | MS.gene048346:CDS | 40.0% | |
| TAAAGTTGCTGATGAGCTTC+AGG | + | chr8.3:34398208-34398227 | MS.gene048346:CDS | 40.0% | |
| TCATATTCAAAACCTCGCCT+TGG | - | chr8.3:34398175-34398194 | None:intergenic | 40.0% | |
| TCCAATTTGCAAGACGAACT+CGG | + | chr8.3:34396154-34396173 | MS.gene048346:CDS | 40.0% | |
| TCGAATCTCTTCGTCTTTCA+CGG | - | chr8.3:34397613-34397632 | None:intergenic | 40.0% | |
| TCTTCAACTAGATGCTGAGA+AGG | + | chr8.3:34398253-34398272 | MS.gene048346:CDS | 40.0% | |
| TGCTAGATATTGGGAAACTG+AGG | + | chr8.3:34396102-34396121 | MS.gene048346:CDS | 40.0% | |
| ! | ACGTTTGGTTTCAATCCTTC+TGG | - | chr8.3:34395854-34395873 | None:intergenic | 40.0% |
| ! | CCTTACTTCCCTTACTCTTT+TGG | - | chr8.3:34396317-34396336 | None:intergenic | 40.0% |
| ! | CTTTTGCAGAAAAGCTTGGA+GGG | + | chr8.3:34397654-34397673 | MS.gene048346:CDS | 40.0% |
| ! | TCAACAGCAGAAGTTGATTC+TGG | - | chr8.3:34397759-34397778 | None:intergenic | 40.0% |
| ! | TCTGCAAGGTGCTTATAAAG+TGG | - | chr8.3:34397984-34398003 | None:intergenic | 40.0% |
| ! | TCTTTTGCAGAAAAGCTTGG+AGG | + | chr8.3:34397653-34397672 | MS.gene048346:CDS | 40.0% |
| !! | CAATACGGCCATTGATTCAT+TGG | + | chr8.3:34397035-34397054 | MS.gene048346:CDS | 40.0% |
| !! | TACTGTTGTTGCTTTCTCCT+CGG | - | chr8.3:34397445-34397464 | None:intergenic | 40.0% |
| AAAGGTGATACCGAATGAGG+AGG | + | chr8.3:34397071-34397090 | MS.gene048346:CDS | 45.0% | |
| AACTGATGAACTCCATTCGC+TGG | + | chr8.3:34396648-34396667 | MS.gene048346:CDS | 45.0% | |
| AATCCAATTCGCAAAAGCCG+AGG | + | chr8.3:34396377-34396396 | MS.gene048346:CDS | 45.0% | |
| ACCGAGTTCGTCTTGCAAAT+TGG | - | chr8.3:34396158-34396177 | None:intergenic | 45.0% | |
| CATACCGATCTTTAGCCGAT+AGG | + | chr8.3:34395705-34395724 | MS.gene048346:CDS | 45.0% | |
| CCAAAAGGTGATACCGAATG+AGG | + | chr8.3:34397068-34397087 | MS.gene048346:CDS | 45.0% | |
| CCACCAAGAAAAATGCCAGA+AGG | + | chr8.3:34395836-34395855 | MS.gene048346:CDS | 45.0% | |
| CTCACCTTATCCTCCTCATT+CGG | - | chr8.3:34397084-34397103 | None:intergenic | 45.0% | |
| GAGAAACCAGATGAGACCTT+CGG | + | chr8.3:34398319-34398338 | MS.gene048346:CDS | 45.0% | |
| GATACCGAATGAGGAGGATA+AGG | + | chr8.3:34397077-34397096 | MS.gene048346:CDS | 45.0% | |
| GCAAACGTGACAATGCTTCT+TGG | - | chr8.3:34396252-34396271 | None:intergenic | 45.0% | |
| GTCACGTTTGCAAGAGAAAC+AGG | + | chr8.3:34396261-34396280 | MS.gene048346:CDS | 45.0% | |
| TCGCAACCTTGAAACGGAAA+AGG | + | chr8.3:34396672-34396691 | MS.gene048346:CDS | 45.0% | |
| TCGTCGTCCTCATCCATATA+AGG | - | chr8.3:34395800-34395819 | None:intergenic | 45.0% | |
| TTTACATCTCGTTTCGGCCT+CGG | - | chr8.3:34396397-34396416 | None:intergenic | 45.0% | |
| ! | CCTCATTCGGTATCACCTTT+TGG | - | chr8.3:34397071-34397090 | None:intergenic | 45.0% |
| ! | CCTTCTGGCATTTTTCTTGG+TGG | - | chr8.3:34395839-34395858 | None:intergenic | 45.0% |
| ! | CGTACCTATCGGCTAAAGAT+CGG | - | chr8.3:34395712-34395731 | None:intergenic | 45.0% |
| ! | GGCTTTAACCGTGACAGAAA+TGG | + | chr8.3:34396534-34396553 | MS.gene048346:CDS | 45.0% |
| ! | TTTCGAGGCTGGCTCAAATT+CGG | + | chr8.3:34396513-34396532 | MS.gene048346:CDS | 45.0% |
| ! | TTTGGAGCTTTCGGAACGTT+TGG | - | chr8.3:34395869-34395888 | None:intergenic | 45.0% |
| !! | AGTGGAGTCTTTGGAGCTTT+CGG | - | chr8.3:34395878-34395897 | None:intergenic | 45.0% |
| !! | TGATTTTCTGGTGGCTGCAA+AGG | - | chr8.3:34397705-34397724 | None:intergenic | 45.0% |
| ACACGGTTCTTCGAATCCGA+AGG | - | chr8.3:34398338-34398357 | None:intergenic | 50.0% | |
| CCAAAAGGTGATACCAGACG+AGG | + | chr8.3:34396861-34396880 | MS.gene048346:CDS | 50.0% | |
| CGAAGAACCGTGTTCCTCTA+AGG | + | chr8.3:34398345-34398364 | MS.gene048346:CDS | 50.0% | |
| CGGCTAAAGATCGGTATGCA+CGG | - | chr8.3:34395703-34395722 | None:intergenic | 50.0% | |
| GATACCAGACGAGGACGATA+TGG | + | chr8.3:34396870-34396889 | MS.gene048346:CDS | 50.0% | |
| GGAAACTGAGGGACAGATCA+AGG | + | chr8.3:34396114-34396133 | MS.gene048346:CDS | 50.0% | |
| GGGAATCGACGAACTTCTAG+AGG | + | chr8.3:34397794-34397813 | MS.gene048346:CDS | 50.0% | |
| TCGAATCCGAAGGTCTCATC+TGG | - | chr8.3:34398328-34398347 | None:intergenic | 50.0% | |
| TTGCTGATGAGCTTCAGGCT+GGG | + | chr8.3:34398213-34398232 | MS.gene048346:CDS | 50.0% | |
| ! | CCTCGTCTGGTATCACCTTT+TGG | - | chr8.3:34396864-34396883 | None:intergenic | 50.0% |
| ! | TTGCAAGACGAACTCGGTGA+AGG | + | chr8.3:34396160-34396179 | MS.gene048346:CDS | 50.0% |
| !! | GCTGGTTCGCAACCTTGAAA+CGG | + | chr8.3:34396666-34396685 | MS.gene048346:CDS | 50.0% |
| !! | GGCATTTTTCTTGGTGGCCT+TGG | - | chr8.3:34395833-34395852 | None:intergenic | 50.0% |
| !! | TTTTCTGGTGGCTGCAAAGG+AGG | - | chr8.3:34397702-34397721 | None:intergenic | 50.0% |
| CAAGGTTGCGAACCAGCGAA+TGG | - | chr8.3:34396663-34396682 | None:intergenic | 55.0% | |
| CTCACCATATCGTCCTCGTC+TGG | - | chr8.3:34396877-34396896 | None:intergenic | 55.0% | |
| GAGCCGCTTCTGCCATCAAA+CGG | - | chr8.3:34396219-34396238 | None:intergenic | 55.0% | |
| GGAAAACAGCGCGTTGCTGA+AGG | + | chr8.3:34398031-34398050 | MS.gene048346:CDS | 55.0% | |
| GTTGCTGATGAGCTTCAGGC+TGG | + | chr8.3:34398212-34398231 | MS.gene048346:CDS | 55.0% | |
| TAACGACAGCGACAGCCACA+AGG | + | chr8.3:34395918-34395937 | MS.gene048346:CDS | 55.0% | |
| TGCTGCGAAATTCCAAGGCG+AGG | + | chr8.3:34398160-34398179 | MS.gene048346:CDS | 55.0% | |
| ! | TCGCCGTTTGATGGCAGAAG+CGG | + | chr8.3:34396213-34396232 | MS.gene048346:CDS | 55.0% |
| ! | CGGCCTCGGCTTTTGCGAAT+TGG | - | chr8.3:34396383-34396402 | None:intergenic | 60.0% |
| !! | ACGACGACGAAGCATCGCCA+AGG | + | chr8.3:34395813-34395832 | MS.gene048346:CDS | 60.0% |
| CGAGGAGGCTCGCCGTTTGA+TGG | + | chr8.3:34396204-34396223 | MS.gene048346:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 34395578 | 34398448 | 34395578 | ID=MS.gene048346 |
| chr8.3 | mRNA | 34395578 | 34398448 | 34395578 | ID=MS.gene048346.t1;Parent=MS.gene048346 |
| chr8.3 | exon | 34395578 | 34398448 | 34395578 | ID=MS.gene048346.t1.exon1;Parent=MS.gene048346.t1 |
| chr8.3 | CDS | 34395578 | 34398448 | 34395578 | ID=cds.MS.gene048346.t1;Parent=MS.gene048346.t1 |
| Gene Sequence |
| Protein sequence |
