AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049100.t1 MTR_8g103030 98.635 293 4 0 1 293 1 293 0.0 593
MS.gene049100.t1 MTR_6g488150 60.993 282 58 5 23 293 14 254 2.67e-108 315
MS.gene049100.t1 MTR_2g101500 54.627 335 101 11 1 293 1 326 2.49e-104 307
MS.gene049100.t1 MTR_5g030710 51.274 314 103 10 12 293 40 335 2.81e-95 285
MS.gene049100.t1 MTR_8g067530 50.482 311 107 9 12 293 64 356 1.08e-90 273
MS.gene049100.t1 MTR_5g067350 47.205 322 105 8 12 293 51 347 1.39e-82 253
MS.gene049100.t1 MTR_1g080860 48.214 280 92 8 24 292 15 252 1.90e-72 223
MS.gene049100.t1 MTR_1g093240 47.636 275 95 6 23 293 7 236 4.12e-70 217
MS.gene049100.t1 MTR_7g096090 63.125 160 55 2 135 293 94 250 2.87e-66 207
MS.gene049100.t1 MTR_1g080860 46.667 270 91 8 24 282 15 242 7.92e-65 204
MS.gene049100.t1 MTR_1g070520 62.424 165 51 4 135 292 84 244 1.08e-64 203
MS.gene049100.t1 MTR_1g093240 46.008 263 93 6 23 281 7 224 1.50e-61 195
MS.gene049100.t1 MTR_6g488150 52.709 203 45 5 23 214 14 176 4.12e-56 179
MS.gene049100.t1 MTR_1g093240 42.798 243 90 6 23 261 7 204 1.54e-49 163
MS.gene049100.t1 MTR_1g070830 45.385 260 70 11 24 281 12 201 8.91e-49 161
MS.gene049100.t1 MTR_1g076270 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene049100.t1 MTR_1g093350 47.826 184 67 5 98 281 24 178 3.83e-45 151
MS.gene049100.t1 MTR_1g040675 41.473 258 75 9 24 281 1 182 5.85e-45 150
MS.gene049100.t1 MTR_1g069495 43.169 183 71 3 139 288 81 263 1.73e-37 134
MS.gene049100.t1 MTR_5g067350 38.272 243 89 7 12 214 51 272 6.20e-35 127
MS.gene049100.t1 MTR_1g040675 38.235 238 71 8 24 261 1 162 8.42e-35 124
MS.gene049100.t1 MTR_2g100780 42.442 172 76 5 136 284 92 263 1.20e-32 121
MS.gene049100.t1 MTR_4g011880 49.333 150 45 4 138 285 52 172 2.19e-32 118
MS.gene049100.t1 MTR_2g100780 42.945 163 72 4 136 277 92 254 2.43e-32 120
MS.gene049100.t1 MTR_2g102490 53.636 110 38 3 170 276 84 183 2.48e-31 116
MS.gene049100.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 4.96e-29 112
MS.gene049100.t1 MTR_8g014520 39.241 158 80 2 136 277 103 260 5.33e-29 112
MS.gene049100.t1 MTR_3g106850 30.921 152 79 4 131 277 79 209 2.45e-19 84.7
MS.gene049100.t1 MTR_4g124300 35.811 148 63 5 135 276 61 182 5.12e-18 80.5
MS.gene049100.t1 MTR_7g110790 36.090 133 59 4 146 274 45 155 1.97e-16 75.5
MS.gene049100.t1 MTR_1g085750 37.607 117 57 2 165 281 76 176 2.82e-16 75.5
MS.gene049100.t1 MTR_4g115075 30.769 143 62 3 141 275 93 206 4.40e-14 70.1
MS.gene049100.t1 MTR_1g109510 28.571 161 85 4 114 274 38 168 9.88e-12 62.8
MS.gene049100.t1 MTR_1g085750 36.082 97 47 1 165 261 76 157 3.99e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049100.t1 AT4G29080 55.864 324 91 11 1 293 3 305 8.04e-102 300
MS.gene049100.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene049100.t1 AT2G22670 48.993 298 97 7 24 292 47 318 2.85e-84 256
MS.gene049100.t1 AT2G22670 48.993 298 97 7 24 292 64 335 3.19e-84 256
MS.gene049100.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene049100.t1 AT2G22670 48.322 298 97 8 24 292 47 316 5.02e-81 248
MS.gene049100.t1 AT5G65670 46.128 297 110 6 24 292 61 335 6.07e-74 230
MS.gene049100.t1 AT5G65670 45.819 299 110 7 24 292 61 337 4.98e-72 225
MS.gene049100.t1 AT4G14550 48.913 276 87 9 24 293 1 228 5.46e-70 216
MS.gene049100.t1 AT3G04730 53.778 225 76 5 93 292 14 235 1.91e-69 215
MS.gene049100.t1 AT3G23050 49.301 286 81 12 24 293 6 243 1.61e-68 213
MS.gene049100.t1 AT1G04250 45.956 272 100 6 21 292 4 228 1.51e-65 205
MS.gene049100.t1 AT4G14550 47.727 264 84 9 24 281 1 216 7.32e-63 198
MS.gene049100.t1 AT4G14550 44.444 243 81 9 24 260 1 195 1.22e-49 163
MS.gene049100.t1 AT3G23050 45.059 253 76 11 24 261 6 210 1.03e-48 161
MS.gene049100.t1 AT1G04240 41.313 259 75 10 24 282 5 186 9.95e-47 155
MS.gene049100.t1 AT1G04240 41.473 258 74 10 24 281 105 285 2.87e-45 155
MS.gene049100.t1 AT5G43700 52.667 150 47 6 133 282 57 182 2.44e-42 144
MS.gene049100.t1 AT3G23030 48.993 149 45 2 133 281 53 170 4.11e-39 135
MS.gene049100.t1 AT2G33310 44.068 177 69 4 133 287 69 237 6.25e-39 137
MS.gene049100.t1 AT3G15540 37.313 268 91 9 23 288 5 197 1.06e-38 135
MS.gene049100.t1 AT3G23030 48.993 149 45 2 133 281 140 257 1.79e-38 136
MS.gene049100.t1 AT1G04550 45.946 185 65 7 133 293 63 236 4.79e-38 134
MS.gene049100.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene049100.t1 AT2G33310 44.068 177 68 5 133 287 69 236 2.17e-37 133
MS.gene049100.t1 AT1G52830 36.726 226 100 6 56 281 2 184 1.04e-36 129
MS.gene049100.t1 AT4G14560 49.020 153 49 5 126 278 41 164 3.00e-35 125
MS.gene049100.t1 AT4G28640 44.910 167 70 4 133 282 81 242 7.41e-35 126
MS.gene049100.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.17e-34 126
MS.gene049100.t1 AT3G16500 40.964 166 75 4 130 277 92 252 2.24e-34 126
MS.gene049100.t1 AT3G16500 40.964 166 75 4 130 277 92 252 3.01e-34 125
MS.gene049100.t1 AT1G15580 44.366 142 49 2 135 276 48 159 4.69e-34 122
MS.gene049100.t1 AT4G28640 44.848 165 69 4 133 280 81 240 1.43e-33 124
MS.gene049100.t1 AT1G04100 41.916 167 68 4 142 281 95 259 6.05e-31 117
MS.gene049100.t1 AT5G25890 36.250 160 74 3 125 284 39 170 1.25e-29 110
MS.gene049100.t1 AT1G51950 42.683 164 67 6 136 277 92 250 3.91e-29 112
MS.gene049100.t1 AT4G14550 40.513 195 65 8 24 212 1 150 3.96e-28 107
MS.gene049100.t1 AT3G23050 41.429 210 60 11 24 218 6 167 1.84e-27 105
MS.gene049100.t1 AT3G17600 37.931 145 64 5 130 274 36 154 9.61e-23 92.4
MS.gene049100.t1 AT2G46990 39.423 104 47 1 173 276 85 172 7.18e-19 82.4
MS.gene049100.t1 AT3G62100 38.393 112 48 3 169 275 74 169 1.11e-18 82.0
MS.gene049100.t1 AT2G46990 40.449 89 37 1 173 261 85 157 2.51e-14 69.7
MS.gene049100.t1 AT3G62100 37.755 98 40 3 169 261 74 155 7.19e-14 68.2
MS.gene049100.t1 AT4G32280 35.294 102 45 2 173 274 118 198 4.25e-13 67.4
MS.gene049100.t1 AT4G32280 35.294 102 45 2 173 274 160 240 1.17e-12 67.0
MS.gene049100.t1 AT1G15050 31.579 133 74 2 147 279 35 150 5.89e-12 63.2
MS.gene049100.t1 AT1G15050 31.579 133 74 2 147 279 67 182 1.31e-11 62.8
MS.gene049100.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8
MS.gene049100.t1 AT2G01200 33.043 115 60 2 165 279 91 188 1.33e-11 62.8

Find 81 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACCAATGCTTCTCTCTT+TGG 0.185471 4.1:-2922588 None:intergenic
CTCTCTTTGGTTTCCATTGA+AGG 0.229858 4.1:-2922575 None:intergenic
TCACCACCACCTTTAGCTTT+AGG 0.284997 4.1:-2922815 None:intergenic
GCTATTACTGATGGAGGTTC+TGG 0.289483 4.1:+2922725 MS.gene049100:CDS
AGATCCACTTTCCTCAAGTA+TGG 0.291849 4.1:-2924170 None:intergenic
GGCCTCCAATCCGATCTTTC+CGG 0.299985 4.1:+2924054 MS.gene049100:CDS
GAGTCACCAGAGAGAGAAAA+TGG 0.324653 4.1:+2922653 MS.gene049100:CDS
GCCTCAGTTCAGTAGCTTTG+AGG 0.337674 4.1:-2922613 None:intergenic
CCAGGTCAATGTGGCTCATA+TGG 0.347339 4.1:+2924881 MS.gene049100:intron
GTGAAACCATTGAGTTCTTC+CGG 0.369117 4.1:-2924073 None:intergenic
ATGAAGAGTTCTGAAGCAAT+TGG 0.391710 4.1:+2925234 MS.gene049100:CDS
AGCATCAGAGAAACCCCTTT+TGG 0.393944 4.1:-2922705 None:intergenic
TTGTTATCATGCAAAGGCTT+TGG 0.395919 4.1:-2922902 None:intergenic
TTCTCTGATGCTATTACTGA+TGG 0.411709 4.1:+2922716 MS.gene049100:CDS
TGGTGGTGTGCTTAAGAGTT+TGG 0.414516 4.1:+2922673 MS.gene049100:CDS
TTATGGAGAGGTTCCTTCAA+TGG 0.424280 4.1:+2922562 MS.gene049100:CDS
GTGCCAGTGGAACAAGGTTA+TGG 0.429339 4.1:+2922545 MS.gene049100:CDS
CTGATGGAGGTTCTGGGAAG+TGG 0.429765 4.1:+2922732 MS.gene049100:CDS
CTCTCTGGTGACTCTGAGCC+TGG 0.432328 4.1:-2922644 None:intergenic
CCGAGTCTTGCAAGAGGTTG+AGG 0.432404 4.1:+2925208 MS.gene049100:CDS
ATCACCATCCTTGTCTTCAT+AGG 0.451715 4.1:-2924978 None:intergenic
ACAACAACTGAAGCAGAGAA+AGG 0.456026 4.1:-2922857 None:intergenic
AGATTGGTGGATCTCCTTCA+TGG 0.457760 4.1:+2924938 MS.gene049100:CDS
CGAATCAGGGCGCAAGTTGT+GGG 0.462809 4.1:+2924029 MS.gene049100:intron
CTATTACTGATGGAGGTTCT+GGG 0.466403 4.1:+2922726 MS.gene049100:CDS
TGCTAGTTGGTGATGTTCCA+TGG 0.467311 4.1:+2925005 MS.gene049100:CDS
TTTGGTGTCTGGTGCCAAAA+GGG 0.469379 4.1:+2922691 MS.gene049100:CDS
TACATATATGCATTCAGACT+TGG 0.476242 4.1:-2924126 None:intergenic
TGGAAATGGTGGATCTGAAG+TGG 0.482511 4.1:+2922763 MS.gene049100:CDS
GCGAATCAGGGCGCAAGTTG+TGG 0.486260 4.1:+2924028 MS.gene049100:intron
GTGCTTAAGAGTTTGGTGTC+TGG 0.488896 4.1:+2922680 MS.gene049100:CDS
TGAAGAGTTCTGAAGCAATT+GGG 0.489200 4.1:+2925235 MS.gene049100:CDS
GTTTGGTGTCTGGTGCCAAA+AGG 0.490914 4.1:+2922690 MS.gene049100:CDS
GAACTGAGGCTAGGGTTACC+AGG 0.498587 4.1:+2922626 MS.gene049100:CDS
AAATCTAAGTGAAAGTAGAT+TGG 0.505003 4.1:+2924922 MS.gene049100:CDS
TTGTTCTCTCCTAAAGCTAA+AGG 0.505328 4.1:+2922806 MS.gene049100:CDS
TGATGGAGGTTCTGGGAAGT+GGG 0.506160 4.1:+2922733 MS.gene049100:CDS
GAAACCAAAGAGAGAAGCAT+TGG 0.506436 4.1:+2922584 MS.gene049100:CDS
ATCTTTCCGGAAGAACTCAA+TGG 0.508429 4.1:+2924067 MS.gene049100:CDS
TTGAGTTCTTCCGGAAAGAT+CGG 0.510793 4.1:-2924064 None:intergenic
ATGAAGACAAGGATGGTGAT+TGG 0.522716 4.1:+2924981 MS.gene049100:CDS
CAACAACTGAAGCAGAGAAA+GGG 0.523720 4.1:-2922856 None:intergenic
GGAAATGGTGGATCTGAAGT+GGG 0.526580 4.1:+2922764 MS.gene049100:CDS
TCTGATGCTATTACTGATGG+AGG 0.531086 4.1:+2922719 MS.gene049100:CDS
TTCCGGAAAGATCGGATTGG+AGG 0.531139 4.1:-2924056 None:intergenic
TCCTGCAGCACCTCGAGCCA+TGG 0.531399 4.1:+2925340 MS.gene049100:intron
AAGAAATCTTACTTTGCAGA+AGG 0.533764 4.1:-2922941 None:intergenic
TTGAGGAAAGTGGATCTGAA+TGG 0.538432 4.1:+2924176 MS.gene049100:CDS
TATGTAAAAGTCAGCATGGA+AGG 0.544619 4.1:+2924143 MS.gene049100:CDS
AAATCTTACTTTGCAGAAGG+AGG 0.546311 4.1:-2922938 None:intergenic
TCTAAGTGAAAGTAGATTGG+TGG 0.547921 4.1:+2924925 MS.gene049100:CDS
TCTCCATAACCTTGTTCCAC+TGG 0.551400 4.1:-2922548 None:intergenic
AAAGCTACTGAACTGAGGCT+AGG 0.551933 4.1:+2922617 MS.gene049100:CDS
AGTTCTGAAGCAATTGGGCT+AGG 0.552508 4.1:+2925240 MS.gene049100:CDS
TGCTCCATACTTGAGGAAAG+TGG 0.552637 4.1:+2924166 MS.gene049100:CDS
AAGCTACTGAACTGAGGCTA+GGG 0.557212 4.1:+2922618 MS.gene049100:CDS
TGTGGTTTGTTATCATGCAA+AGG 0.558132 4.1:-2922908 None:intergenic
GAAGTGGGGTTGTGTAAAGA+TGG 0.559129 4.1:+2922779 MS.gene049100:CDS
TTCTTCCGGAAAGATCGGAT+TGG 0.561734 4.1:-2924059 None:intergenic
TTGGTGTCTGGTGCCAAAAG+GGG 0.565847 4.1:+2922692 MS.gene049100:CDS
AGAAAATAGACGTACTCCCA+TGG 0.566126 4.1:-2925022 None:intergenic
CATATATGTAAAAGTCAGCA+TGG 0.572072 4.1:+2924139 MS.gene049100:CDS
GCTAGTTGGTGATGTTCCAT+GGG 0.572324 4.1:+2925006 MS.gene049100:CDS
TCTCCTAAAGCTAAAGGTGG+TGG 0.583056 4.1:+2922812 MS.gene049100:CDS
CCTCAACCTCTTGCAAGACT+CGG 0.585897 4.1:-2925208 None:intergenic
TTACATTTCTCCATGGCTCG+AGG 0.588053 4.1:-2925350 None:intergenic
TCAGGGCGCAAGTTGTGGGA+TGG 0.592117 4.1:+2924033 MS.gene049100:intron
GCACATATTCTGAACCATGA+AGG 0.604244 4.1:-2924952 None:intergenic
GATGGTGATTGGATGCTAGT+TGG 0.610602 4.1:+2924992 MS.gene049100:CDS
TGGAAGGTGCTCCATACTTG+AGG 0.612400 4.1:+2924159 MS.gene049100:CDS
GCAGAAGGAGGAGAAACATG+TGG 0.623815 4.1:-2922926 None:intergenic
ATGTCTGTGCCAGTGGAACA+AGG 0.641481 4.1:+2922539 MS.gene049100:CDS
TTCTCTCCTAAAGCTAAAGG+TGG 0.652446 4.1:+2922809 MS.gene049100:CDS
TTGACAAATCCAGGTCAATG+TGG 0.662027 4.1:+2924872 MS.gene049100:intron
TGTGCTTACCTATGAAGACA+AGG 0.670147 4.1:+2924970 MS.gene049100:CDS
TGTTCACCGAGTCTTGCAAG+AGG 0.674237 4.1:+2925202 MS.gene049100:CDS
GAAATGGTGGATCTGAAGTG+GGG 0.680430 4.1:+2922765 MS.gene049100:CDS
CTTACCTATGAAGACAAGGA+TGG 0.683773 4.1:+2924974 MS.gene049100:CDS
AGTGGAACAAGGTTATGGAG+AGG 0.703860 4.1:+2922550 MS.gene049100:CDS
TCACCAGAGAGAGAAAATGG+TGG 0.713194 4.1:+2922656 MS.gene049100:CDS
ACCTCAAAGCTACTGAACTG+AGG 0.714271 4.1:+2922612 MS.gene049100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTTAAGTTTAACATT+TGG - chr4.1:2924522-2924541 None:intergenic 15.0%
!!! GATGTTATTTTTGTTATTTT+AGG + chr4.1:2925178-2925197 MS.gene049100:intron 15.0%
!!! GATTTTAATATAACAAACAT+TGG - chr4.1:2925271-2925290 None:intergenic 15.0%
!!! TCATTTTATTTAAGCAAAAA+AGG - chr4.1:2924835-2924854 None:intergenic 15.0%
!! AAGTAAGATTTCTTCTTATT+TGG + chr4.1:2922950-2922969 MS.gene049100:intron 20.0%
!!! ACTTGTTGTATCTTTTATTA+TGG - chr4.1:2923419-2923438 None:intergenic 20.0%
!!! ATTGATAGTTAGACTTATTT+TGG + chr4.1:2925101-2925120 MS.gene049100:intron 20.0%
!!! TGATTTCTTTTTTCTCAATA+CGG + chr4.1:2923998-2924017 MS.gene049100:intron 20.0%
! AAATCTAAGTGAAAGTAGAT+TGG + chr4.1:2924922-2924941 MS.gene049100:CDS 25.0%
! AACATGCAGAAGAAAATTTA+AGG - chr4.1:2923752-2923771 None:intergenic 25.0%
! AGATTTATATTATCATGCGT+TGG - chr4.1:2923928-2923947 None:intergenic 25.0%
! CGATCATAAATTCAAAACTA+AGG - chr4.1:2923965-2923984 None:intergenic 25.0%
! GCAAAAATATTTGAAAATCG+AGG + chr4.1:2924411-2924430 MS.gene049100:intron 25.0%
! TGACATGAATTTATGAACAT+CGG + chr4.1:2924596-2924615 MS.gene049100:intron 25.0%
! TTGATTCTAACATTGTTCAT+AGG - chr4.1:2925127-2925146 None:intergenic 25.0%
!! TCTATTGTTGGTTGAAATTA+TGG + chr4.1:2924690-2924709 MS.gene049100:intron 25.0%
!!! ACTTTTTTCACAAGCTATTA+TGG + chr4.1:2923474-2923493 MS.gene049100:intron 25.0%
!!! AGTTTTGAATTTATGATCGT+TGG + chr4.1:2923966-2923985 MS.gene049100:intron 25.0%
!!! GATACCTTCATATTTTTTGT+CGG + chr4.1:2925299-2925318 MS.gene049100:intron 25.0%
!!! TTCACATAGATAGTTACTTT+TGG + chr4.1:2923721-2923740 MS.gene049100:intron 25.0%
!!! TTCTACATTAATTTGTCAGT+TGG + chr4.1:2923240-2923259 MS.gene049100:intron 25.0%
AAGAAATCTTACTTTGCAGA+AGG - chr4.1:2922944-2922963 None:intergenic 30.0%
AGATATTTGTGATCATGCAT+CGG - chr4.1:2924324-2924343 None:intergenic 30.0%
CATATATGTAAAAGTCAGCA+TGG + chr4.1:2924139-2924158 MS.gene049100:CDS 30.0%
GATTCCGACAAAAAATATGA+AGG - chr4.1:2925306-2925325 None:intergenic 30.0%
TACATATATGCATTCAGACT+TGG - chr4.1:2924129-2924148 None:intergenic 30.0%
TATGTGCTCTAGTAAATTTC+AGG + chr4.1:2923672-2923691 MS.gene049100:intron 30.0%
TCAAATAAGCCAATCCAAAT+AGG + chr4.1:2923576-2923595 MS.gene049100:intron 30.0%
TCAACCAACAATAGAGAAAA+TGG - chr4.1:2924685-2924704 None:intergenic 30.0%
TGCATGTATTTATATGTCAC+AGG + chr4.1:2924736-2924755 MS.gene049100:intron 30.0%
! GTTGAATCTGATGTTTCTTT+TGG + chr4.1:2923146-2923165 MS.gene049100:intron 30.0%
! TATATTCTTATGTTAGCCTC+TGG + chr4.1:2923619-2923638 MS.gene049100:intron 30.0%
! TCAACCATTTTCTCTATTGT+TGG + chr4.1:2924678-2924697 MS.gene049100:intron 30.0%
!! ATGTTTTTGTTGACAAATCC+AGG + chr4.1:2924863-2924882 MS.gene049100:intron 30.0%
!!! AATCACTTTTGAGACTGTTT+GGG - chr4.1:2923540-2923559 None:intergenic 30.0%
!!! GAATGGTTTTAGCTCTTATA+GGG + chr4.1:2924193-2924212 MS.gene049100:CDS 30.0%
!!! TAATCACTTTTGAGACTGTT+TGG - chr4.1:2923541-2923560 None:intergenic 30.0%
!!! TGAATGGTTTTAGCTCTTAT+AGG + chr4.1:2924192-2924211 MS.gene049100:CDS 30.0%
AAATCTTACTTTGCAGAAGG+AGG - chr4.1:2922941-2922960 None:intergenic 35.0%
ACATCAAGACACACAGATAA+TGG - chr4.1:2924296-2924315 None:intergenic 35.0%
ATAATTAGCAACAACCACCA+AGG - chr4.1:2923602-2923621 None:intergenic 35.0%
ATCTTCATTCTCAACCATGT+TGG - chr4.1:2924783-2924802 None:intergenic 35.0%
CAACTGTACTGTCAAAGTAT+AGG - chr4.1:2924572-2924591 None:intergenic 35.0%
CATCAAGACACACAGATAAT+GGG - chr4.1:2924295-2924314 None:intergenic 35.0%
GCATGATCACAAATATCTCT+CGG + chr4.1:2924326-2924345 MS.gene049100:intron 35.0%
TATGTAAAAGTCAGCATGGA+AGG + chr4.1:2924143-2924162 MS.gene049100:CDS 35.0%
TCTAAGTGAAAGTAGATTGG+TGG + chr4.1:2924925-2924944 MS.gene049100:CDS 35.0%
TTCTCTGATGCTATTACTGA+TGG + chr4.1:2922716-2922735 MS.gene049100:CDS 35.0%
TTGTTATCATGCAAAGGCTT+TGG - chr4.1:2922905-2922924 None:intergenic 35.0%
TTGTTCTCTCCTAAAGCTAA+AGG + chr4.1:2922806-2922825 MS.gene049100:CDS 35.0%
! ATACGGTTTTTATGCGAATC+AGG + chr4.1:2924015-2924034 MS.gene049100:intron 35.0%
! ATGGTTGAAAGAGAGTGTAT+TGG - chr4.1:2924666-2924685 None:intergenic 35.0%
! ATGTAGAAGATCTTCTAGAG+AGG - chr4.1:2923228-2923247 None:intergenic 35.0%
! CAAAGGTATGACTACTGTAT+AGG - chr4.1:2924808-2924827 None:intergenic 35.0%
! GAAAGAGAGTGTATTGGTAA+CGG - chr4.1:2924660-2924679 None:intergenic 35.0%
! TAAGCAAAAAAGGCATGCAA+AGG - chr4.1:2924825-2924844 None:intergenic 35.0%
! TACGGTTTTTATGCGAATCA+GGG + chr4.1:2924016-2924035 MS.gene049100:intron 35.0%
! TGTAGAAGATCTTCTAGAGA+GGG - chr4.1:2923227-2923246 None:intergenic 35.0%
! TGTGCTTTGTTGATTGATGT+TGG + chr4.1:2923011-2923030 MS.gene049100:intron 35.0%
!! ATGAAGAGTTCTGAAGCAAT+TGG + chr4.1:2925234-2925253 MS.gene049100:CDS 35.0%
!! TGAAGAGTTCTGAAGCAATT+GGG + chr4.1:2925235-2925254 MS.gene049100:CDS 35.0%
!! TGTGGTTTGTTATCATGCAA+AGG - chr4.1:2922911-2922930 None:intergenic 35.0%
AAATCGAGGTGTGTCTTGAA+TGG + chr4.1:2924425-2924444 MS.gene049100:intron 40.0%
AAGACATGGAAAAGGCTAAC+TGG - chr4.1:2923858-2923877 None:intergenic 40.0%
AATATCTCTCGGTGATGTGA+TGG + chr4.1:2924337-2924356 MS.gene049100:intron 40.0%
ACAACAACTGAAGCAGAGAA+AGG - chr4.1:2922860-2922879 None:intergenic 40.0%
ACTGGACAAAATCAAATCCC+AGG - chr4.1:2923840-2923859 None:intergenic 40.0%
AGAAAATAGACGTACTCCCA+TGG - chr4.1:2925025-2925044 None:intergenic 40.0%
AGATCCACTTTCCTCAAGTA+TGG - chr4.1:2924173-2924192 None:intergenic 40.0%
AGCATCAGAAGACATGGAAA+AGG - chr4.1:2923866-2923885 None:intergenic 40.0%
ATCACCATCCTTGTCTTCAT+AGG - chr4.1:2924981-2925000 None:intergenic 40.0%
ATCTTTCCGGAAGAACTCAA+TGG + chr4.1:2924067-2924086 MS.gene049100:CDS 40.0%
ATGAAGACAAGGATGGTGAT+TGG + chr4.1:2924981-2925000 MS.gene049100:CDS 40.0%
CAAACCAATGCTTCTCTCTT+TGG - chr4.1:2922591-2922610 None:intergenic 40.0%
CAACAACTGAAGCAGAGAAA+GGG - chr4.1:2922859-2922878 None:intergenic 40.0%
CTATTACTGATGGAGGTTCT+GGG + chr4.1:2922726-2922745 MS.gene049100:CDS 40.0%
CTGGACAAAATCAAATCCCA+GGG - chr4.1:2923839-2923858 None:intergenic 40.0%
CTTACCTATGAAGACAAGGA+TGG + chr4.1:2924974-2924993 MS.gene049100:CDS 40.0%
GCACATATTCTGAACCATGA+AGG - chr4.1:2924955-2924974 None:intergenic 40.0%
GTGAAACCATTGAGTTCTTC+CGG - chr4.1:2924076-2924095 None:intergenic 40.0%
TCTGATGCTATTACTGATGG+AGG + chr4.1:2922719-2922738 MS.gene049100:CDS 40.0%
TGAGTATGCGGATAATTCAG+AGG + chr4.1:2923180-2923199 MS.gene049100:intron 40.0%
TGTGCTTACCTATGAAGACA+AGG + chr4.1:2924970-2924989 MS.gene049100:CDS 40.0%
TTATGGAGAGGTTCCTTCAA+TGG + chr4.1:2922562-2922581 MS.gene049100:CDS 40.0%
TTCTCTCCTAAAGCTAAAGG+TGG + chr4.1:2922809-2922828 MS.gene049100:CDS 40.0%
TTGACAAATCCAGGTCAATG+TGG + chr4.1:2924872-2924891 MS.gene049100:intron 40.0%
TTGAGGAAAGTGGATCTGAA+TGG + chr4.1:2924176-2924195 MS.gene049100:CDS 40.0%
TTGAGTTCTTCCGGAAAGAT+CGG - chr4.1:2924067-2924086 None:intergenic 40.0%
! CTCTCTTTGGTTTCCATTGA+AGG - chr4.1:2922578-2922597 None:intergenic 40.0%
! GAAACCAAAGAGAGAAGCAT+TGG + chr4.1:2922584-2922603 MS.gene049100:CDS 40.0%
! GCATCAGCATCATTCTTTTG+AGG - chr4.1:2924101-2924120 None:intergenic 40.0%
! GCGACTTTTACATTTCTCCA+TGG - chr4.1:2925360-2925379 None:intergenic 40.0%
! TATGTCACAGGTTCTGATTG+TGG + chr4.1:2924748-2924767 MS.gene049100:intron 40.0%
!! AAGCAGAGAAAGGGTTTTGT+TGG - chr4.1:2922850-2922869 None:intergenic 40.0%
!! AAGTGGGTTTTGTCTGGAAA+TGG + chr4.1:2922749-2922768 MS.gene049100:CDS 40.0%
AAAGCTACTGAACTGAGGCT+AGG + chr4.1:2922617-2922636 MS.gene049100:CDS 45.0%
AAATCCCAGGGCATACACAA+GGG - chr4.1:2923827-2923846 None:intergenic 45.0%
AACAACCACCAAGGCCTATT+TGG - chr4.1:2923593-2923612 None:intergenic 45.0%
AAGCCAATCCAAATAGGCCT+TGG + chr4.1:2923582-2923601 MS.gene049100:intron 45.0%
AAGCTACTGAACTGAGGCTA+GGG + chr4.1:2922618-2922637 MS.gene049100:CDS 45.0%
ACCTCAAAGCTACTGAACTG+AGG + chr4.1:2922612-2922631 MS.gene049100:CDS 45.0%
AGTGGAACAAGGTTATGGAG+AGG + chr4.1:2922550-2922569 MS.gene049100:CDS 45.0%
CCATGTCTTCTGATGCTGAA+AGG + chr4.1:2923869-2923888 MS.gene049100:intron 45.0%
CCTTTCAGCATCAGAAGACA+TGG - chr4.1:2923872-2923891 None:intergenic 45.0%
GAAGTGGGGTTGTGTAAAGA+TGG + chr4.1:2922779-2922798 MS.gene049100:CDS 45.0%
GAGTCACCAGAGAGAGAAAA+TGG + chr4.1:2922653-2922672 MS.gene049100:CDS 45.0%
GATGGTGATTGGATGCTAGT+TGG + chr4.1:2924992-2925011 MS.gene049100:CDS 45.0%
GCGGATAATTCAGAGGAAGA+GGG + chr4.1:2923187-2923206 MS.gene049100:intron 45.0%
GCTATTACTGATGGAGGTTC+TGG + chr4.1:2922725-2922744 MS.gene049100:CDS 45.0%
GTGTAGAGCAAGTGAGTATG+CGG + chr4.1:2923168-2923187 MS.gene049100:intron 45.0%
TCACCAGAGAGAGAAAATGG+TGG + chr4.1:2922656-2922675 MS.gene049100:CDS 45.0%
TCTCCATAACCTTGTTCCAC+TGG - chr4.1:2922551-2922570 None:intergenic 45.0%
TCTCCTAAAGCTAAAGGTGG+TGG + chr4.1:2922812-2922831 MS.gene049100:CDS 45.0%
TGCGGATAATTCAGAGGAAG+AGG + chr4.1:2923186-2923205 MS.gene049100:intron 45.0%
TGCTCCATACTTGAGGAAAG+TGG + chr4.1:2924166-2924185 MS.gene049100:CDS 45.0%
TTACATTTCTCCATGGCTCG+AGG - chr4.1:2925353-2925372 None:intergenic 45.0%
TTAGCCTCTGGTGTAAAAGC+TGG + chr4.1:2923631-2923650 MS.gene049100:intron 45.0%
TTCTCAACCATGTTGGCACT+TGG - chr4.1:2924776-2924795 None:intergenic 45.0%
TTCTTCCGGAAAGATCGGAT+TGG - chr4.1:2924062-2924081 None:intergenic 45.0%
! ACACCACCATTTTCTCTCTC+TGG - chr4.1:2922662-2922681 None:intergenic 45.0%
! AGATTGGTGGATCTCCTTCA+TGG + chr4.1:2924938-2924957 MS.gene049100:CDS 45.0%
! AGCATCAGAGAAACCCCTTT+TGG - chr4.1:2922708-2922727 None:intergenic 45.0%
! TGGTGGTGTGCTTAAGAGTT+TGG + chr4.1:2922673-2922692 MS.gene049100:CDS 45.0%
!! ACTCACTTGTTGATTCGCAC+TGG + chr4.1:2923315-2923334 MS.gene049100:intron 45.0%
!! ACTTGTTGATTCGCACTGGT+TGG + chr4.1:2923319-2923338 MS.gene049100:intron 45.0%
!! AGTTCTGAAGCAATTGGGCT+AGG + chr4.1:2925240-2925259 MS.gene049100:CDS 45.0%
!! GAAATGGTGGATCTGAAGTG+GGG + chr4.1:2922765-2922784 MS.gene049100:CDS 45.0%
!! GCTAGTTGGTGATGTTCCAT+GGG + chr4.1:2925006-2925025 MS.gene049100:CDS 45.0%
!! GGAAATGGTGGATCTGAAGT+GGG + chr4.1:2922764-2922783 MS.gene049100:CDS 45.0%
!! GTGCTTAAGAGTTTGGTGTC+TGG + chr4.1:2922680-2922699 MS.gene049100:CDS 45.0%
!! TCACCACCACCTTTAGCTTT+AGG - chr4.1:2922818-2922837 None:intergenic 45.0%
!! TGCTAGTTGGTGATGTTCCA+TGG + chr4.1:2925005-2925024 MS.gene049100:CDS 45.0%
!! TGGAAATGGTGGATCTGAAG+TGG + chr4.1:2922763-2922782 MS.gene049100:CDS 45.0%
!! TGGGTTTTGTCTGGAAATGG+TGG + chr4.1:2922752-2922771 MS.gene049100:CDS 45.0%
!! TTTGGTGTCTGGTGCCAAAA+GGG + chr4.1:2922691-2922710 MS.gene049100:CDS 45.0%
ATGTCTGTGCCAGTGGAACA+AGG + chr4.1:2922539-2922558 MS.gene049100:CDS 50.0%
CAAATCCCAGGGCATACACA+AGG - chr4.1:2923828-2923847 None:intergenic 50.0%
CCAATCCAAATAGGCCTTGG+TGG + chr4.1:2923585-2923604 MS.gene049100:intron 50.0%
CCACCAAGGCCTATTTGGAT+TGG - chr4.1:2923588-2923607 None:intergenic 50.0%
CCAGGTCAATGTGGCTCATA+TGG + chr4.1:2924881-2924900 MS.gene049100:intron 50.0%
CCATATGAGCCACATTGACC+TGG - chr4.1:2924884-2924903 None:intergenic 50.0%
CCTCAACCTCTTGCAAGACT+CGG - chr4.1:2925211-2925230 None:intergenic 50.0%
GCAGAAGGAGGAGAAACATG+TGG - chr4.1:2922929-2922948 None:intergenic 50.0%
GTGCCAGTGGAACAAGGTTA+TGG + chr4.1:2922545-2922564 MS.gene049100:CDS 50.0%
TGTTCACCGAGTCTTGCAAG+AGG + chr4.1:2925202-2925221 MS.gene049100:CDS 50.0%
! AACGCCAGCTTTTACACCAG+AGG - chr4.1:2923638-2923657 None:intergenic 50.0%
! GCCTCAGTTCAGTAGCTTTG+AGG - chr4.1:2922616-2922635 None:intergenic 50.0%
! TTCCGGAAAGATCGGATTGG+AGG - chr4.1:2924059-2924078 None:intergenic 50.0%
! TTGGTGTCTGGTGCCAAAAG+GGG + chr4.1:2922692-2922711 MS.gene049100:CDS 50.0%
!! GTTTGGTGTCTGGTGCCAAA+AGG + chr4.1:2922690-2922709 MS.gene049100:CDS 50.0%
!! TCTGGGAAGTGGGTTTTGTC+TGG + chr4.1:2922743-2922762 MS.gene049100:CDS 50.0%
!! TGATGGAGGTTCTGGGAAGT+GGG + chr4.1:2922733-2922752 MS.gene049100:CDS 50.0%
!! TGGAAGGTGCTCCATACTTG+AGG + chr4.1:2924159-2924178 MS.gene049100:CDS 50.0%
CCGAGTCTTGCAAGAGGTTG+AGG + chr4.1:2925208-2925227 MS.gene049100:CDS 55.0%
CGAATCAGGGCGCAAGTTGT+GGG + chr4.1:2924029-2924048 MS.gene049100:intron 55.0%
GAACTGAGGCTAGGGTTACC+AGG + chr4.1:2922626-2922645 MS.gene049100:CDS 55.0%
GGCCTCCAATCCGATCTTTC+CGG + chr4.1:2924054-2924073 MS.gene049100:CDS 55.0%
TGTGCCCTTGTGTATGCCCT+GGG + chr4.1:2923820-2923839 MS.gene049100:intron 55.0%
TTGTGCCCTTGTGTATGCCC+TGG + chr4.1:2923819-2923838 MS.gene049100:intron 55.0%
! TGTGGTGCCAAGTGCCAACA+TGG + chr4.1:2924766-2924785 MS.gene049100:intron 55.0%
!! CTGATGGAGGTTCTGGGAAG+TGG + chr4.1:2922732-2922751 MS.gene049100:CDS 55.0%
CTCTCTGGTGACTCTGAGCC+TGG - chr4.1:2922647-2922666 None:intergenic 60.0%
GCGAATCAGGGCGCAAGTTG+TGG + chr4.1:2924028-2924047 MS.gene049100:intron 60.0%
TCAGGGCGCAAGTTGTGGGA+TGG + chr4.1:2924033-2924052 MS.gene049100:intron 60.0%
TCCTGCAGCACCTCGAGCCA+TGG + chr4.1:2925340-2925359 MS.gene049100:intron 65.0%
!! TCCATGGCTCGAGGTGCTGC+AGG - chr4.1:2925344-2925363 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 2922539 2925385 2922539 ID=MS.gene049100
chr4.1 mRNA 2922539 2925385 2922539 ID=MS.gene049100.t1;Parent=MS.gene049100
chr4.1 exon 2922539 2922951 2922539 ID=MS.gene049100.t1.exon1;Parent=MS.gene049100.t1
chr4.1 CDS 2922539 2922951 2922539 ID=cds.MS.gene049100.t1;Parent=MS.gene049100.t1
chr4.1 exon 2924037 2924260 2924037 ID=MS.gene049100.t1.exon2;Parent=MS.gene049100.t1
chr4.1 CDS 2924037 2924260 2924037 ID=cds.MS.gene049100.t1;Parent=MS.gene049100.t1
chr4.1 exon 2924885 2925029 2924885 ID=MS.gene049100.t1.exon3;Parent=MS.gene049100.t1
chr4.1 CDS 2924885 2925029 2924885 ID=cds.MS.gene049100.t1;Parent=MS.gene049100.t1
chr4.1 exon 2925200 2925261 2925200 ID=MS.gene049100.t1.exon4;Parent=MS.gene049100.t1
chr4.1 CDS 2925200 2925261 2925200 ID=cds.MS.gene049100.t1;Parent=MS.gene049100.t1
chr4.1 exon 2925348 2925385 2925348 ID=MS.gene049100.t1.exon5;Parent=MS.gene049100.t1
chr4.1 CDS 2925348 2925385 2925348 ID=cds.MS.gene049100.t1;Parent=MS.gene049100.t1
Gene Sequence

>MS.gene049100

ATGTCTGTGCCAGTGGAACAAGGTTATGGAGAGGTTCCTTCAATGGAAACCAAAGAGAGAAGCATTGGTTTGAACCTCAAAGCTACTGAACTGAGGCTAGGGTTACCAGGCTCAGAGTCACCAGAGAGAGAAAATGGTGGTGTGCTTAAGAGTTTGGTGTCTGGTGCCAAAAGGGGTTTCTCTGATGCTATTACTGATGGAGGTTCTGGGAAGTGGGTTTTGTCTGGAAATGGTGGATCTGAAGTGGGGTTGTGTAAAGATGGAAACTTGTTCTCTCCTAAAGCTAAAGGTGGTGGTGAATGTAATAACCAACAAAACCCTTTCTCTGCTTCAGTTGTTGTTAAAGAAACTGTTACACACTCTCCAAAGCCTTTGCATGATAACAAACCACATGTTTCTCCTCCTTCTGCAAAGTAAGATTTCTTCTTATTTGGTTGTATGTTATGTTTCTCTTAGTGTTTGTGTATGTTGATGTGCTTTGTTGATTGATGTTGGCTCTTTGTTCATTGAAATATTTTTTGTTTATTTGTCTTGTCAGTTATCACTATTTAGTGTTTATAGTTTCTTCTGTGAGTTTACAAAATGTTGAAAACAAGAACAAAATTATGTTGAATCTGATGTTTCTTTTGGTGTAGAGCAAGTGAGTATGCGGATAATTCAGAGGAAGAGGGATTTTTTTTTTTCTCCCTCTCTAGAAGATCTTCTACATTAATTTGTCAGTTGGAAATTTGAACAGAAGTCAACTAACTCACTTTTTTTTTTTTTTACAAGAACAAACTCACTTGTTGATTCGCACTGGTTGGATAAACAACTTAATTAAGTGCTTATAGCATTAGCACTTATCTTATATCTTATAAGTGCTTATATATAGCTATTTCCATAATAAAAGATACAACAAGTTCAAAGTGTTTTCATTTAAGCAATTTAAGCTATAAACTTTTTTCACAAGCTATTATGGAGAGCTTATGCTCATGTCATAAGTTGTATCTATAAGCTCTCCCAAACAGTCTCAAAAGTGATTATACTAGTAGATAAGCTCAAATAAGCCAATCCAAATAGGCCTTGGTGGTTGTTGCTAATTATATTCTTATGTTAGCCTCTGGTGTAAAAGCTGGCGTTGTTACTTGTAGTTGTATGTGCTCTAGTAAATTTCAGGATTTTCTATGTGCTAATCTTCAAAATTTCACATAGATAGTTACTTTTGGTTATTCCTTAAATTTTCTTCTGCATGTTGCATTCAATATCTTAAAAGTTTAAAATTTTATCTTAAAAGTTTTTTCTTGTGCCCTTGTGTATGCCCTGGGATTTGATTTTGTCCAGTTAGCCTTTTCCATGTCTTCTGATGCTGAAAGGTTTTTACTCAGCTTCTGTATTCATTGATTTTCACCAACGCATGATAATATAAATCTGTTTTAGCTTGATTCCTTAGTTTTGAATTTATGATCGTTGGCTTTAAAATTGATTTCTTTTTTCTCAATACGGTTTTTATGCGAATCAGGGCGCAAGTTGTGGGATGGCCTCCAATCCGATCTTTCCGGAAGAACTCAATGGTTTCACAGCCTCAAAAGAATGATGCTGATGCAGAAGCCAAGTCTGAATGCATATATGTAAAAGTCAGCATGGAAGGTGCTCCATACTTGAGGAAAGTGGATCTGAATGGTTTTAGCTCTTATAGGGAACTTTCTTCAGCACTCGAAAAGATGTTCAGTTGTTTTACAATCAGTGAGTTTTTATCTACTTGTTATCGTCTTCTCCCATTATCTGTGTGTCTTGATGTTTTCACCGATGCATGATCACAAATATCTCTCGGTGATGTGATGGTGATCATTCTAGTAAATGTATTGTACTTTGACGAACATTTTAGTTAAATGAGCAAAAATATTTGAAAATCGAGGTGTGTCTTGAATGGCTCAATAAATTCTTTACTAGAAAAAGTTCTTTATATAATTAATTGCATATGAAAAAGAAAACGTAATGAAACCAAATGTTAAACTTAAATAGTTAGCTCAAACACACAGACACACATTTTGCCTATACTTTGACAGTACAGTTGTTGATGACATGAATTTATGAACATCGGTCAATGTCAGTGTTGTATAGTGTTTCAATTGAAGAGAACCGTTACCAATACACTCTCTTTCAACCATTTTCTCTATTGTTGGTTGAAATTATGGCATATAAAAACTATTATAGAATATGCATGTATTTATATGTCACAGGTTCTGATTGTGGTGCCAAGTGCCAACATGGTTGAGAATGAAGATTACCTATACAGTAGTCATACCTTTGCATGCCTTTTTTGCTTAAATAAAATGATTCTGATGATGTTTTTGTTGACAAATCCAGGTCAATGTGGCTCATATGGAGTTTCTTGTCGAGAAAAAAATCTAAGTGAAAGTAGATTGGTGGATCTCCTTCATGGTTCAGAATATGTGCTTACCTATGAAGACAAGGATGGTGATTGGATGCTAGTTGGTGATGTTCCATGGGAGTACGTCTATTTTCTTATATATAATTCATAATTCACTACATGAATTAACAATGATTAGCTATGTTTGCTGAATTGATAGTTAGACTTATTTTGGCCTATGAACAATGTTAGAATCAACAAAGAGTGATTTAATTCTGATATCTGAAATGATGTTATTTTTGTTATTTTAGGATGTTCACCGAGTCTTGCAAGAGGTTGAGGATAATGAAGAGTTCTGAAGCAATTGGGCTAGGTAAGACCAATGTTTGTTATATTAAAATCTCATGTATGATACCTTCATATTTTTTGTCGGAATCATGTATACTCTTATTCCTGCAGCACCTCGAGCCATGGAGAAATGTAAAAGTCGCAACTAG
Protein sequence

>MS.gene049100.t1

MSVPVEQGYGEVPSMETKERSIGLNLKATELRLGLPGSESPERENGGVLKSLVSGAKRGFSDAITDGGSGKWVLSGNGGSEVGLCKDGNLFSPKAKGGGECNNQQNPFSASVVVKETVTHSPKPLHDNKPHVSPPSAKAQVVGWPPIRSFRKNSMVSQPQKNDADAEAKSECIYVKVSMEGAPYLRKVDLNGFSSYRELSSALEKMFSCFTISQCGSYGVSCREKNLSESRLVDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCKRLRIMKSSEAIGLAPRAMEKCKSRN