AlfalfaGEDB | MS.gene049541

M. sativa cultivar XinJiangDaYe / MS.gene049541

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049541.t1	KEH34477.1	89.5	133	14	0	1	133	29	161	4.20E-59	237.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049541.t1	Q9FJK3	44.9	127	69	1	1	127	29	154	8.3e-24	111.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049541.t1	A0A072UY35	89.5	133	14	0	1	133	29	161	3.0e-59	237.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene049541.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049541	MS.gene20657	0.802175	6.36E-49	-1.69E-46
MS.gene049541	MS.gene30139	0.808517	2.98E-50	-1.69E-46
MS.gene049541	MS.gene34615	0.806819	6.83E-50	-1.69E-46
MS.gene049541	MS.gene38444	0.820185	7.84E-53	-1.69E-46
MS.gene049541	MS.gene41508	0.809285	2.04E-50	-1.69E-46
MS.gene049541	MS.gene41509	0.844134	9.12E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049541.t1	MTR_3g467080	89.474	133	14	0	1	133	29	161	2.36e-86	249
MS.gene049541.t1	MTR_4g063790	78.947	133	28	0	1	133	29	161	1.55e-73	217
MS.gene049541.t1	MTR_3g465410	78.195	133	29	0	1	133	1	133	6.11e-73	214
MS.gene049541.t1	MTR_3g466980	51.969	127	58	2	1	126	29	153	1.68e-40	134
MS.gene049541.t1	MTR_3g065100	45.968	124	66	1	4	127	32	154	2.14e-34	119
MS.gene049541.t1	MTR_3g031100	44.697	132	73	0	1	132	29	160	8.62e-34	118
MS.gene049541.t1	MTR_3g031240	44.961	129	71	0	1	129	29	157	3.26e-31	110
MS.gene049541.t1	MTR_2g035580	46.032	126	67	1	2	127	30	154	2.86e-30	107
MS.gene049541.t1	MTR_2g016210	40.909	132	78	0	1	132	29	160	3.72e-30	108
MS.gene049541.t1	MTR_4g032290	40.769	130	77	0	4	133	32	161	3.74e-28	103
MS.gene049541.t1	MTR_5g075380	37.302	126	78	1	1	126	29	153	4.68e-28	101
MS.gene049541.t1	MTR_2g035610	49.451	91	46	0	4	94	32	122	1.24e-27	99.0
MS.gene049541.t1	MTR_4g032620	36.508	126	80	0	1	126	29	154	4.49e-27	100
MS.gene049541.t1	MTR_4g032260	37.398	123	77	0	6	128	34	156	5.03e-27	98.6
MS.gene049541.t1	MTR_4g031910	40.000	125	75	0	4	128	32	156	2.56e-26	98.6
MS.gene049541.t1	MTR_8g036130	39.695	131	77	1	1	129	29	159	2.19e-25	96.7
MS.gene049541.t1	MTR_3g466830	38.400	125	76	1	1	125	28	151	5.21e-23	88.6
MS.gene049541.t1	MTR_3g466900	33.333	126	83	1	4	129	2	126	1.41e-19	79.0
MS.gene049541.t1	MTR_1g077320	33.858	127	80	3	4	129	32	155	1.17e-18	77.0
MS.gene049541.t1	MTR_3g466890	34.400	125	81	1	2	126	29	152	7.75e-18	75.1
MS.gene049541.t1	MTR_3g466930	33.333	126	83	1	4	129	2	126	1.08e-17	73.9
MS.gene049541.t1	MTR_5g047580	33.333	129	84	2	1	129	29	155	1.45e-17	74.3
MS.gene049541.t1	MTR_4g028720	31.746	126	84	2	4	129	32	155	6.86e-17	72.4
MS.gene049541.t1	MTR_1g077300	30.769	130	86	2	4	133	32	157	7.16e-17	72.4
MS.gene049541.t1	MTR_7g011950	30.952	126	85	2	4	129	32	155	9.16e-17	72.4
MS.gene049541.t1	MTR_1g090783	30.233	129	88	2	1	129	28	154	1.21e-16	72.0
MS.gene049541.t1	MTR_7g055800	42.857	84	33	1	3	86	18	86	3.05e-16	68.9
MS.gene049541.t1	MTR_1g077390	34.848	132	78	4	1	129	29	155	3.52e-16	70.9
MS.gene049541.t1	MTR_1g084950	29.921	127	85	3	4	129	32	155	1.90e-15	68.9
MS.gene049541.t1	MTR_7g055940	42.105	76	29	1	3	78	18	78	1.02e-13	62.4
MS.gene049541.t1	MTR_1g090697	29.134	127	86	3	1	126	29	152	8.71e-13	62.0
MS.gene049541.t1	MTR_4g084780	53.704	54	25	0	46	99	1	54	2.14e-12	60.8
MS.gene049541.t1	MTR_1g090710	27.481	131	90	3	1	129	29	156	6.96e-12	59.3
MS.gene049541.t1	MTR_4g028800	48.889	45	23	0	1	45	27	71	1.17e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049541.t1	AT5G48670	43.548	124	69	1	4	127	32	154	7.23e-30	110
MS.gene049541.t1	AT5G27810	52.809	89	42	0	1	89	1	89	4.70e-29	102
MS.gene049541.t1	AT1G65300	38.636	132	79	2	2	133	29	158	7.30e-23	90.9
MS.gene049541.t1	AT1G65330	37.705	122	75	1	1	122	28	148	9.52e-20	82.4
MS.gene049541.t1	AT3G05860	38.710	124	71	2	1	122	29	149	1.61e-19	80.5
MS.gene049541.t1	AT3G05860	39.062	128	65	3	1	122	29	149	3.10e-19	80.5
MS.gene049541.t1	AT3G05860	39.062	128	65	3	1	122	29	149	4.42e-19	80.5
MS.gene049541.t1	AT5G26650	33.333	129	85	1	1	129	27	154	5.59e-18	78.6
MS.gene049541.t1	AT1G31640	36.364	132	70	4	2	127	29	152	3.71e-16	73.9
MS.gene049541.t1	AT5G26630	30.579	121	83	1	6	126	34	153	6.04e-16	71.2
MS.gene049541.t1	AT5G27960	31.008	129	88	1	1	129	27	154	8.68e-16	72.4
MS.gene049541.t1	AT1G22590	29.545	132	76	2	1	129	29	146	4.06e-13	62.8
MS.gene049541.t1	AT2G28700	30.328	122	84	1	1	122	29	149	4.71e-13	64.7
MS.gene049541.t1	AT1G31630	31.496	127	79	4	3	126	30	151	1.19e-12	63.5

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGGTCATTTCCTGCTTTC+TGG	0.066665	3.2:+57741703	None:intergenic
ATTCATACCACAGAATTTAA+TGG	0.196701	3.2:-57741665	MS.gene049541:CDS
CAGGTTTGGCCATCAGATTC+AGG	0.280803	3.2:-57741860	MS.gene049541:CDS
GTTCAGGAGTTTGCCTGGAC+TGG	0.382826	3.2:-57741816	MS.gene049541:CDS
CTGAATCTGATGGCCAAACC+TGG	0.394292	3.2:+57741861	None:intergenic
GAAGTGTGATATCCAGGTTC+AGG	0.406864	3.2:-57741832	MS.gene049541:CDS
GATGAAATTAGCACTCTTTG+CGG	0.413383	3.2:-57741929	MS.gene049541:CDS
AATGAAATGATGAATGATAT+TGG	0.442815	3.2:+57741685	None:intergenic
TCCAGGTTCAGGAGTTTGCC+TGG	0.447666	3.2:-57741821	MS.gene049541:CDS
ACAATCCTCAAGCCCAGGTT+TGG	0.451431	3.2:-57741874	MS.gene049541:CDS
TATTCTTGAGGCAAAGCATT+GGG	0.452810	3.2:-57741763	MS.gene049541:CDS
GAGGACAAATCATTAAGATC+AGG	0.474701	3.2:+57741626	None:intergenic
GTGTTAGAAGTGTGATATCC+AGG	0.495852	3.2:-57741838	MS.gene049541:CDS
TCCAGGCAAACTCCTGAACC+TGG	0.517673	3.2:+57741820	None:intergenic
AAGGAAGGAAACCAGAAAGC+AGG	0.520416	3.2:-57741714	MS.gene049541:CDS
CAAATTCTCAGCAATAAAAG+AGG	0.520681	3.2:+57741607	None:intergenic
TAAATATCCATTAAATTCTG+TGG	0.554522	3.2:+57741658	None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG	0.555152	3.2:-57741764	MS.gene049541:CDS
GATGGCCAAACCTGGGCTTG+AGG	0.558655	3.2:+57741869	None:intergenic
TTGCTCGTAAATTATTGCAC+CGG	0.562136	3.2:+57741898	None:intergenic
GCAAAACAATCCTCAAGCCC+AGG	0.573782	3.2:-57741879	MS.gene049541:CDS
TGAATCTGATGGCCAAACCT+GGG	0.601638	3.2:+57741862	None:intergenic
GAGGTCGATCAAAAGATGAA+AGG	0.605953	3.2:-57741581	MS.gene049541:CDS
ATGAGCAGATGATGAAACAA+AGG	0.611511	3.2:-57741733	MS.gene049541:CDS
ACTCTTTGCGGAATAGAAGC+CGG	0.612299	3.2:-57741917	MS.gene049541:CDS
CTTCTAACACCTGAATCTGA+TGG	0.613550	3.2:+57741851	None:intergenic
TATTGCTGAGAATTTGAGGG+AGG	0.639350	3.2:-57741600	MS.gene049541:CDS
GCAGATGATGAAACAAAGGA+AGG	0.642572	3.2:-57741729	MS.gene049541:CDS
TGGATCAAGAGGTATTCTTG+AGG	0.709854	3.2:-57741775	MS.gene049541:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGAAATGATGAATGATAT+TGG	+	chr3.2:57741821-57741840	None:intergenic	20.0%
!!	TAAATATCCATTAAATTCTG+TGG	+	chr3.2:57741848-57741867	None:intergenic	20.0%
!	ATTCATACCACAGAATTTAA+TGG	-	chr3.2:57741838-57741857	MS.gene049541:CDS	25.0%
!!	TTTTATTGCTGAGAATTTGA+GGG	-	chr3.2:57741900-57741919	MS.gene049541:CDS	25.0%
	ACTGGAACAAAGTAAAAAGA+TGG	-	chr3.2:57741705-57741724	MS.gene049541:CDS	30.0%
	CAAATTCTCAGCAATAAAAG+AGG	+	chr3.2:57741899-57741918	None:intergenic	30.0%
!	CTTTTATTGCTGAGAATTTG+AGG	-	chr3.2:57741899-57741918	MS.gene049541:CDS	30.0%
	ATGAGCAGATGATGAAACAA+AGG	-	chr3.2:57741770-57741789	MS.gene049541:CDS	35.0%
	GAGGACAAATCATTAAGATC+AGG	+	chr3.2:57741880-57741899	None:intergenic	35.0%
	GATGAAATTAGCACTCTTTG+CGG	-	chr3.2:57741574-57741593	MS.gene049541:CDS	35.0%
	GGAACAAAGTAAAAAGATGG+TGG	-	chr3.2:57741708-57741727	MS.gene049541:CDS	35.0%
	TTGCTCGTAAATTATTGCAC+CGG	+	chr3.2:57741608-57741627	None:intergenic	35.0%
!	TATTCTTGAGGCAAAGCATT+GGG	-	chr3.2:57741740-57741759	MS.gene049541:CDS	35.0%
!	TTTTTACTTTGTTCCAGTCC+AGG	+	chr3.2:57741703-57741722	None:intergenic	35.0%
!!	TAAAAAGATGGTGGATCAAG+AGG	-	chr3.2:57741717-57741736	MS.gene049541:CDS	35.0%
	ATTGGTCATTTCCTGCTTTC+TGG	+	chr3.2:57741803-57741822	None:intergenic	40.0%
	CTTCTAACACCTGAATCTGA+TGG	+	chr3.2:57741655-57741674	None:intergenic	40.0%
	GAGGTCGATCAAAAGATGAA+AGG	-	chr3.2:57741922-57741941	MS.gene049541:CDS	40.0%
	GCAGATGATGAAACAAAGGA+AGG	-	chr3.2:57741774-57741793	MS.gene049541:CDS	40.0%
	GTATTCTTGAGGCAAAGCAT+TGG	-	chr3.2:57741739-57741758	MS.gene049541:CDS	40.0%
	TATTGCTGAGAATTTGAGGG+AGG	-	chr3.2:57741903-57741922	MS.gene049541:CDS	40.0%
!	GTGTTAGAAGTGTGATATCC+AGG	-	chr3.2:57741665-57741684	MS.gene049541:CDS	40.0%
!!	TGGATCAAGAGGTATTCTTG+AGG	-	chr3.2:57741728-57741747	MS.gene049541:CDS	40.0%
	AAGGAAGGAAACCAGAAAGC+AGG	-	chr3.2:57741789-57741808	MS.gene049541:CDS	45.0%
	ACTCTTTGCGGAATAGAAGC+CGG	-	chr3.2:57741586-57741605	MS.gene049541:CDS	45.0%
	GAAGTGTGATATCCAGGTTC+AGG	-	chr3.2:57741671-57741690	MS.gene049541:CDS	45.0%
	TGAATCTGATGGCCAAACCT+GGG	+	chr3.2:57741644-57741663	None:intergenic	45.0%
	ACAATCCTCAAGCCCAGGTT+TGG	-	chr3.2:57741629-57741648	MS.gene049541:CDS	50.0%
	CAGGTTTGGCCATCAGATTC+AGG	-	chr3.2:57741643-57741662	MS.gene049541:CDS	50.0%
	CTGAATCTGATGGCCAAACC+TGG	+	chr3.2:57741645-57741664	None:intergenic	50.0%
	GCAAAACAATCCTCAAGCCC+AGG	-	chr3.2:57741624-57741643	MS.gene049541:CDS	50.0%
	GTTCAGGAGTTTGCCTGGAC+TGG	-	chr3.2:57741687-57741706	MS.gene049541:CDS	55.0%
	TCCAGGCAAACTCCTGAACC+TGG	+	chr3.2:57741686-57741705	None:intergenic	55.0%
	TCCAGGTTCAGGAGTTTGCC+TGG	-	chr3.2:57741682-57741701	MS.gene049541:CDS	55.0%
	GATGGCCAAACCTGGGCTTG+AGG	+	chr3.2:57741637-57741656	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	57741562	57741963	57741562	ID=MS.gene049541
chr3.2	mRNA	57741562	57741963	57741562	ID=MS.gene049541.t1;Parent=MS.gene049541
chr3.2	exon	57741562	57741963	57741562	ID=MS.gene049541.t1.exon1;Parent=MS.gene049541.t1
chr3.2	CDS	57741562	57741963	57741562	ID=cds.MS.gene049541.t1;Parent=MS.gene049541.t1

Gene Sequence

>MS.gene049541

ATGAAGAAGATTGATGAAATTAGCACTCTTTGCGGAATAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGTGTTAGAAGTGTGATATCCAGGTTCAGGAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGATGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACCTGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTTAATCGAAGATTGA

Protein sequence

>MS.gene049541.t1

MKKIDEISTLCGIEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQMMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKPDLNDLSSFIAENLREVDQKMKGMLIED