Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41508.t1 | KEH34477.1 | 88.2 | 161 | 19 | 0 | 1 | 161 | 1 | 161 | 6.20E-73 | 283.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41508.t1 | Q9FJK3 | 47.1 | 155 | 81 | 1 | 1 | 155 | 1 | 154 | 4.1e-33 | 142.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41508.t1 | A0A072UY35 | 88.2 | 161 | 19 | 0 | 1 | 161 | 1 | 161 | 4.4e-73 | 283.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene41508.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049541 | MS.gene41508 | 0.809285 | 2.04E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene41508 | 0.834304 | 3.24E-56 | -1.69E-46 |
| MS.gene052447 | MS.gene41508 | 0.804693 | 1.91E-49 | -1.69E-46 |
| MS.gene055017 | MS.gene41508 | 0.807156 | 5.80E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene41508 | 0.803387 | 3.57E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41508.t1 | MTR_3g467080 | 88.199 | 161 | 19 | 0 | 1 | 161 | 1 | 161 | 2.21e-105 | 298 |
| MS.gene41508.t1 | MTR_4g063790 | 80.124 | 161 | 32 | 0 | 1 | 161 | 1 | 161 | 4.67e-92 | 265 |
| MS.gene41508.t1 | MTR_3g465410 | 78.947 | 133 | 28 | 0 | 29 | 161 | 1 | 133 | 3.26e-73 | 216 |
| MS.gene41508.t1 | MTR_3g466980 | 53.247 | 154 | 71 | 1 | 1 | 154 | 1 | 153 | 3.60e-55 | 173 |
| MS.gene41508.t1 | MTR_3g031240 | 46.250 | 160 | 86 | 0 | 1 | 160 | 1 | 160 | 3.43e-45 | 146 |
| MS.gene41508.t1 | MTR_3g031100 | 47.771 | 157 | 82 | 0 | 1 | 157 | 1 | 157 | 2.47e-44 | 146 |
| MS.gene41508.t1 | MTR_3g065100 | 48.101 | 158 | 81 | 1 | 1 | 158 | 1 | 157 | 5.96e-42 | 140 |
| MS.gene41508.t1 | MTR_2g016210 | 41.667 | 156 | 91 | 0 | 5 | 160 | 5 | 160 | 1.35e-40 | 137 |
| MS.gene41508.t1 | MTR_2g035580 | 46.452 | 155 | 82 | 1 | 1 | 155 | 1 | 154 | 3.20e-40 | 133 |
| MS.gene41508.t1 | MTR_5g075380 | 41.558 | 154 | 89 | 1 | 1 | 154 | 1 | 153 | 3.44e-38 | 128 |
| MS.gene41508.t1 | MTR_8g036130 | 40.506 | 158 | 92 | 1 | 1 | 156 | 1 | 158 | 3.14e-36 | 125 |
| MS.gene41508.t1 | MTR_4g032620 | 38.312 | 154 | 95 | 0 | 1 | 154 | 1 | 154 | 4.51e-36 | 125 |
| MS.gene41508.t1 | MTR_4g032290 | 42.236 | 161 | 93 | 0 | 1 | 161 | 1 | 161 | 1.42e-33 | 118 |
| MS.gene41508.t1 | MTR_3g466830 | 39.735 | 151 | 90 | 1 | 3 | 153 | 2 | 151 | 1.92e-33 | 116 |
| MS.gene41508.t1 | MTR_4g031910 | 41.615 | 161 | 94 | 0 | 1 | 161 | 1 | 161 | 5.36e-33 | 117 |
| MS.gene41508.t1 | MTR_2g035610 | 48.361 | 122 | 63 | 0 | 1 | 122 | 1 | 122 | 2.06e-32 | 112 |
| MS.gene41508.t1 | MTR_4g032260 | 38.365 | 159 | 98 | 0 | 1 | 159 | 1 | 159 | 4.97e-31 | 110 |
| MS.gene41508.t1 | MTR_5g047580 | 36.943 | 157 | 97 | 2 | 1 | 157 | 1 | 155 | 2.02e-29 | 105 |
| MS.gene41508.t1 | MTR_1g077390 | 38.272 | 162 | 92 | 4 | 1 | 159 | 1 | 157 | 8.19e-28 | 101 |
| MS.gene41508.t1 | MTR_3g466890 | 35.484 | 155 | 99 | 1 | 3 | 157 | 2 | 155 | 1.74e-27 | 100 |
| MS.gene41508.t1 | MTR_1g090783 | 34.839 | 155 | 99 | 2 | 3 | 157 | 2 | 154 | 7.46e-27 | 99.4 |
| MS.gene41508.t1 | MTR_1g077320 | 35.802 | 162 | 100 | 3 | 1 | 161 | 1 | 159 | 1.87e-25 | 95.5 |
| MS.gene41508.t1 | MTR_4g028720 | 36.025 | 161 | 101 | 2 | 1 | 161 | 1 | 159 | 2.19e-25 | 95.5 |
| MS.gene41508.t1 | MTR_7g011950 | 34.161 | 161 | 104 | 2 | 1 | 161 | 1 | 159 | 9.61e-24 | 91.3 |
| MS.gene41508.t1 | MTR_1g090697 | 32.911 | 158 | 102 | 3 | 1 | 157 | 1 | 155 | 1.05e-23 | 91.3 |
| MS.gene41508.t1 | MTR_1g077300 | 35.404 | 161 | 100 | 2 | 1 | 161 | 1 | 157 | 1.50e-23 | 90.9 |
| MS.gene41508.t1 | MTR_1g090710 | 33.333 | 159 | 101 | 3 | 1 | 157 | 1 | 156 | 3.58e-23 | 89.7 |
| MS.gene41508.t1 | MTR_1g084950 | 34.810 | 158 | 99 | 3 | 1 | 157 | 1 | 155 | 5.07e-22 | 86.7 |
| MS.gene41508.t1 | MTR_3g466900 | 32.540 | 126 | 84 | 1 | 32 | 157 | 2 | 126 | 3.30e-19 | 79.0 |
| MS.gene41508.t1 | MTR_4g028800 | 47.945 | 73 | 36 | 1 | 1 | 73 | 1 | 71 | 1.11e-18 | 75.9 |
| MS.gene41508.t1 | MTR_3g466930 | 32.540 | 126 | 84 | 1 | 32 | 157 | 2 | 126 | 2.48e-17 | 73.9 |
| MS.gene41508.t1 | MTR_7g055800 | 36.364 | 99 | 48 | 1 | 16 | 114 | 3 | 86 | 6.52e-16 | 69.3 |
| MS.gene41508.t1 | MTR_5g047560 | 29.299 | 157 | 93 | 3 | 1 | 157 | 1 | 139 | 1.09e-14 | 67.4 |
| MS.gene41508.t1 | MTR_4g019670 | 29.412 | 136 | 89 | 3 | 16 | 148 | 19 | 150 | 2.21e-14 | 69.7 |
| MS.gene41508.t1 | MTR_7g055790 | 35.780 | 109 | 52 | 2 | 1 | 109 | 59 | 149 | 2.84e-14 | 66.6 |
| MS.gene41508.t1 | MTR_7g055940 | 35.165 | 91 | 44 | 1 | 16 | 106 | 3 | 78 | 1.81e-13 | 62.4 |
| MS.gene41508.t1 | MTR_7g106510 | 28.070 | 114 | 82 | 0 | 5 | 118 | 8 | 121 | 1.16e-12 | 65.1 |
| MS.gene41508.t1 | MTR_4g084780 | 55.556 | 54 | 24 | 0 | 74 | 127 | 1 | 54 | 1.71e-12 | 61.6 |
| MS.gene41508.t1 | MTR_3g093900 | 26.056 | 142 | 96 | 2 | 16 | 153 | 19 | 155 | 6.58e-12 | 62.8 |
| MS.gene41508.t1 | MTR_6g018920 | 30.088 | 113 | 72 | 3 | 1 | 107 | 1 | 112 | 6.82e-12 | 62.4 |
| MS.gene41508.t1 | MTR_1g114730 | 29.310 | 116 | 75 | 3 | 1 | 110 | 1 | 115 | 1.67e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41508.t1 | AT5G48670 | 48.077 | 156 | 78 | 2 | 1 | 155 | 1 | 154 | 1.31e-37 | 131 |
| MS.gene41508.t1 | AT1G65330 | 41.892 | 148 | 85 | 1 | 3 | 150 | 2 | 148 | 5.00e-30 | 110 |
| MS.gene41508.t1 | AT3G05860 | 51.515 | 99 | 48 | 0 | 1 | 99 | 1 | 99 | 1.15e-29 | 107 |
| MS.gene41508.t1 | AT1G65300 | 39.735 | 151 | 90 | 1 | 5 | 155 | 4 | 153 | 1.99e-29 | 108 |
| MS.gene41508.t1 | AT3G05860 | 51.515 | 99 | 48 | 0 | 1 | 99 | 1 | 99 | 2.21e-29 | 108 |
| MS.gene41508.t1 | AT3G05860 | 51.515 | 99 | 48 | 0 | 1 | 99 | 1 | 99 | 4.02e-29 | 107 |
| MS.gene41508.t1 | AT5G27810 | 52.809 | 89 | 42 | 0 | 29 | 117 | 1 | 89 | 1.08e-28 | 102 |
| MS.gene41508.t1 | AT5G26650 | 36.601 | 153 | 96 | 1 | 5 | 157 | 3 | 154 | 1.53e-25 | 100 |
| MS.gene41508.t1 | AT5G27960 | 35.948 | 153 | 97 | 1 | 5 | 157 | 3 | 154 | 1.62e-24 | 97.1 |
| MS.gene41508.t1 | AT1G31630 | 36.875 | 160 | 83 | 5 | 3 | 154 | 2 | 151 | 1.81e-23 | 94.4 |
| MS.gene41508.t1 | AT1G22590 | 33.750 | 160 | 89 | 2 | 1 | 157 | 1 | 146 | 2.01e-23 | 90.5 |
| MS.gene41508.t1 | AT1G31640 | 38.272 | 162 | 86 | 4 | 3 | 158 | 2 | 155 | 3.59e-23 | 94.7 |
| MS.gene41508.t1 | AT2G28700 | 32.667 | 150 | 100 | 1 | 1 | 150 | 1 | 149 | 3.78e-21 | 88.2 |
| MS.gene41508.t1 | AT5G26630 | 33.548 | 155 | 102 | 1 | 1 | 155 | 1 | 154 | 2.06e-20 | 84.3 |
| MS.gene41508.t1 | AT5G06500 | 33.333 | 147 | 88 | 3 | 1 | 147 | 1 | 137 | 1.01e-18 | 80.1 |
| MS.gene41508.t1 | AT2G40210 | 35.644 | 101 | 65 | 0 | 1 | 101 | 1 | 101 | 3.52e-14 | 68.9 |
| MS.gene41508.t1 | AT5G26580 | 38.614 | 101 | 48 | 1 | 1 | 101 | 1 | 87 | 7.25e-14 | 68.2 |
| MS.gene41508.t1 | AT3G57230 | 28.395 | 162 | 101 | 4 | 1 | 161 | 1 | 148 | 2.08e-11 | 60.5 |
| MS.gene41508.t1 | AT3G57230 | 28.395 | 162 | 101 | 4 | 1 | 161 | 1 | 148 | 2.08e-11 | 60.5 |
| MS.gene41508.t1 | AT3G57230 | 29.825 | 114 | 76 | 2 | 1 | 113 | 1 | 111 | 3.98e-11 | 58.2 |
Find 31 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTCATTTCCTGCTTTC+TGG | 0.066665 | 3.1:+51380148 | None:intergenic |
| ATTCATACTACAGAATTTAA+TGG | 0.196701 | 3.1:-51380110 | MS.gene41508:CDS |
| CAAGTTTGGCCATCAGATTC+AGG | 0.225208 | 3.1:-51380305 | MS.gene41508:CDS |
| CTGAATGTTATCCTCCTCTT+CGG | 0.350265 | 3.1:+51380622 | None:intergenic |
| ACAATCCTCAAGCACAAGTT+TGG | 0.390704 | 3.1:-51380319 | MS.gene41508:CDS |
| GTTCAGAAGTTTGCCTGGAC+TGG | 0.392828 | 3.1:-51380261 | MS.gene41508:CDS |
| TGAATGTTATCCTCCTCTTC+GGG | 0.395416 | 3.1:+51380623 | None:intergenic |
| GATGAAATTAGCACTCTTTG+CGG | 0.422591 | 3.1:-51380374 | MS.gene41508:CDS |
| GGGTTAGAAGTGTGATATCT+AGG | 0.436600 | 3.1:-51380283 | MS.gene41508:CDS |
| AATGAAATGATGAATGATAT+TGG | 0.442815 | 3.1:+51380130 | None:intergenic |
| TATTCTTGAGGCAAAGCATT+GGG | 0.452810 | 3.1:-51380208 | MS.gene41508:CDS |
| TCTAGGTTCAGAAGTTTGCC+TGG | 0.467579 | 3.1:-51380266 | MS.gene41508:CDS |
| AAGTTTGGCCATCAGATTCA+GGG | 0.497829 | 3.1:-51380304 | MS.gene41508:CDS |
| CACTATCATGGCTAGAGGTA+AGG | 0.508320 | 3.1:-51380671 | None:intergenic |
| AAGGAAGGAAACCAGAAAGC+AGG | 0.520416 | 3.1:-51380159 | MS.gene41508:CDS |
| GGAGGATAACATTCAGAAAG+AGG | 0.525464 | 3.1:-51380615 | MS.gene41508:CDS |
| CAAATTCTCAGCAACAAAAG+AGG | 0.527893 | 3.1:+51380052 | None:intergenic |
| GTATTCTTGAGGCAAAGCAT+TGG | 0.555152 | 3.1:-51380209 | MS.gene41508:CDS |
| TTGCTCGTAAATTATTGCAC+CGG | 0.562136 | 3.1:+51380343 | None:intergenic |
| GATGGCCAAACTTGTGCTTG+AGG | 0.578295 | 3.1:+51380314 | None:intergenic |
| ACTCTTTGCGGAGTAGAAGC+CGG | 0.579730 | 3.1:-51380362 | MS.gene41508:CDS |
| GAGGTCGATCAAAAGATGAA+AGG | 0.605953 | 3.1:-51380026 | MS.gene41508:CDS |
| CTTCTAACCCCTGAATCTGA+TGG | 0.608216 | 3.1:+51380296 | None:intergenic |
| ATGAGCAGTTGATGAAACAA+AGG | 0.608842 | 3.1:-51380178 | MS.gene41508:CDS |
| ACATAACATGTGACCCGAAG+AGG | 0.618667 | 3.1:-51380636 | MS.gene41508:CDS |
| GCAGTTGATGAAACAAAGGA+AGG | 0.627441 | 3.1:-51380174 | MS.gene41508:CDS |
| AGTTTGGCCATCAGATTCAG+GGG | 0.628974 | 3.1:-51380303 | MS.gene41508:CDS |
| TGTTGCTGAGAATTTGAGGG+AGG | 0.633462 | 3.1:-51380045 | MS.gene41508:CDS |
| ATCCTCACTATCATGGCTAG+AGG | 0.659018 | 3.1:-51380676 | None:intergenic |
| TAACATGTGACCCGAAGAGG+AGG | 0.659370 | 3.1:-51380633 | MS.gene41508:CDS |
| TGGATCAAGAGGTATTCTTG+AGG | 0.709854 | 3.1:-51380220 | MS.gene41508:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATGGATTTTTTTTAATA+GGG | - | chr3.1:51380259-51380278 | MS.gene41508:CDS | 10.0% |
| !! | TATAGGTTATATATTGTTTA+TGG | - | chr3.1:51380243-51380262 | MS.gene41508:CDS | 15.0% |
| !!! | GTTTATGGATTTTTTTTAAT+AGG | - | chr3.1:51380258-51380277 | MS.gene41508:CDS | 15.0% |
| !!! | TAAATTTGGAATTTTACTAT+AGG | - | chr3.1:51380226-51380245 | MS.gene41508:CDS | 15.0% |
| !! | AATGAAATGATGAATGATAT+TGG | + | chr3.1:51380544-51380563 | None:intergenic | 20.0% |
| !! | ATTCATACTACAGAATTTAA+TGG | - | chr3.1:51380561-51380580 | MS.gene41508:intron | 20.0% |
| ACATTCAGAAAGAGGAAAAA+TGG | - | chr3.1:51380064-51380083 | MS.gene41508:CDS | 30.0% | |
| ACTGGAACAAAGTAAAAAGA+TGG | - | chr3.1:51380428-51380447 | MS.gene41508:intron | 30.0% | |
| TATGTATGATATGCAAAGAG+AGG | + | chr3.1:51380100-51380119 | None:intergenic | 30.0% | |
| ! | TTTTGTTGCTGAGAATTTGA+GGG | - | chr3.1:51380623-51380642 | MS.gene41508:CDS | 30.0% |
| CAAATTCTCAGCAACAAAAG+AGG | + | chr3.1:51380622-51380641 | None:intergenic | 35.0% | |
| GATGAAATTAGCACTCTTTG+CGG | - | chr3.1:51380297-51380316 | MS.gene41508:CDS | 35.0% | |
| GGAACAAAGTAAAAAGATGG+TGG | - | chr3.1:51380431-51380450 | MS.gene41508:intron | 35.0% | |
| TTGCTCGTAAATTATTGCAC+CGG | + | chr3.1:51380331-51380350 | None:intergenic | 35.0% | |
| ! | CTTTTGTTGCTGAGAATTTG+AGG | - | chr3.1:51380622-51380641 | MS.gene41508:CDS | 35.0% |
| ! | TATTCTTGAGGCAAAGCATT+GGG | - | chr3.1:51380463-51380482 | MS.gene41508:intron | 35.0% |
| ! | TTTTTACTTTGTTCCAGTCC+AGG | + | chr3.1:51380426-51380445 | None:intergenic | 35.0% |
| !! | ATGAGCAGTTGATGAAACAA+AGG | - | chr3.1:51380493-51380512 | MS.gene41508:intron | 35.0% |
| !! | TAAAAAGATGGTGGATCAAG+AGG | - | chr3.1:51380440-51380459 | MS.gene41508:intron | 35.0% |
| AAGTTTGGCCATCAGATTCA+GGG | - | chr3.1:51380367-51380386 | MS.gene41508:CDS | 40.0% | |
| ACAATCCTCAAGCACAAGTT+TGG | - | chr3.1:51380352-51380371 | MS.gene41508:CDS | 40.0% | |
| ATTGGTCATTTCCTGCTTTC+TGG | + | chr3.1:51380526-51380545 | None:intergenic | 40.0% | |
| CTGAATGTTATCCTCCTCTT+CGG | + | chr3.1:51380052-51380071 | None:intergenic | 40.0% | |
| GAGGTCGATCAAAAGATGAA+AGG | - | chr3.1:51380645-51380664 | MS.gene41508:CDS | 40.0% | |
| GGAGGATAACATTCAGAAAG+AGG | - | chr3.1:51380056-51380075 | MS.gene41508:CDS | 40.0% | |
| GTATTCTTGAGGCAAAGCAT+TGG | - | chr3.1:51380462-51380481 | MS.gene41508:intron | 40.0% | |
| TGAATGTTATCCTCCTCTTC+GGG | + | chr3.1:51380051-51380070 | None:intergenic | 40.0% | |
| ! | GGGTTAGAAGTGTGATATCT+AGG | - | chr3.1:51380388-51380407 | MS.gene41508:CDS | 40.0% |
| !! | GCAGTTGATGAAACAAAGGA+AGG | - | chr3.1:51380497-51380516 | MS.gene41508:intron | 40.0% |
| !! | TGGATCAAGAGGTATTCTTG+AGG | - | chr3.1:51380451-51380470 | MS.gene41508:intron | 40.0% |
| AAGGAAGGAAACCAGAAAGC+AGG | - | chr3.1:51380512-51380531 | MS.gene41508:intron | 45.0% | |
| ACATAACATGTGACCCGAAG+AGG | - | chr3.1:51380035-51380054 | MS.gene41508:CDS | 45.0% | |
| AGTTTGGCCATCAGATTCAG+GGG | - | chr3.1:51380368-51380387 | MS.gene41508:CDS | 45.0% | |
| CAAGTTTGGCCATCAGATTC+AGG | - | chr3.1:51380366-51380385 | MS.gene41508:CDS | 45.0% | |
| CTTCTAACCCCTGAATCTGA+TGG | + | chr3.1:51380378-51380397 | None:intergenic | 45.0% | |
| TCTAGGTTCAGAAGTTTGCC+TGG | - | chr3.1:51380405-51380424 | MS.gene41508:intron | 45.0% | |
| TGTTGCTGAGAATTTGAGGG+AGG | - | chr3.1:51380626-51380645 | MS.gene41508:CDS | 45.0% | |
| !!! | TAACTTTTATTTAATAAATT+TGG | - | chr3.1:51380212-51380231 | MS.gene41508:CDS | 5.0% |
| ACTCTTTGCGGAGTAGAAGC+CGG | - | chr3.1:51380309-51380328 | MS.gene41508:CDS | 50.0% | |
| GATGGCCAAACTTGTGCTTG+AGG | + | chr3.1:51380360-51380379 | None:intergenic | 50.0% | |
| GTTCAGAAGTTTGCCTGGAC+TGG | - | chr3.1:51380410-51380429 | MS.gene41508:intron | 50.0% | |
| TAACATGTGACCCGAAGAGG+AGG | - | chr3.1:51380038-51380057 | MS.gene41508:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 51380007 | 51380686 | 51380007 | ID=MS.gene41508 |
| chr3.1 | mRNA | 51380007 | 51380686 | 51380007 | ID=MS.gene41508.t1;Parent=MS.gene41508 |
| chr3.1 | exon | 51380608 | 51380686 | 51380608 | ID=MS.gene41508.t1.exon1;Parent=MS.gene41508.t1 |
| chr3.1 | CDS | 51380608 | 51380686 | 51380608 | ID=cds.MS.gene41508.t1;Parent=MS.gene41508.t1 |
| chr3.1 | exon | 51380007 | 51380413 | 51380007 | ID=MS.gene41508.t1.exon2;Parent=MS.gene41508.t1 |
| chr3.1 | CDS | 51380007 | 51380413 | 51380007 | ID=cds.MS.gene41508.t1;Parent=MS.gene41508.t1 |
| Gene Sequence |
| Protein sequence |
