Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049878.t1 | XP_003629708.1 | 74.6 | 224 | 11 | 3 | 1 | 178 | 47 | 270 | 5.80E-88 | 333.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049878.t1 | O80582 | 41.3 | 138 | 66 | 3 | 42 | 164 | 150 | 287 | 4.6e-25 | 115.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049878.t1 | G7LHZ0 | 74.6 | 224 | 11 | 3 | 1 | 178 | 47 | 270 | 4.2e-88 | 333.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049878 | MS.gene057060 | 0.809253 | 2.07E-50 | -1.69E-46 |
| MS.gene049878 | MS.gene23305 | 0.800179 | 1.63E-48 | -1.69E-46 |
| MS.gene049878 | MS.gene64249 | 0.80252 | 5.40E-49 | -1.69E-46 |
| MS.gene049878 | MS.gene81375 | 0.800643 | 1.31E-48 | -1.69E-46 |
| MS.gene049878 | MS.gene88091 | 0.81214 | 4.92E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049878.t1 | MTR_8g085650 | 74.222 | 225 | 12 | 3 | 1 | 179 | 47 | 271 | 4.25e-111 | 320 |
| MS.gene049878.t1 | MTR_4g011900 | 35.111 | 225 | 95 | 9 | 1 | 175 | 45 | 268 | 5.90e-26 | 102 |
| MS.gene049878.t1 | MTR_2g102370 | 34.348 | 230 | 96 | 9 | 1 | 175 | 43 | 272 | 5.00e-25 | 99.8 |
| MS.gene049878.t1 | MTR_2g102355 | 41.667 | 96 | 50 | 2 | 86 | 175 | 47 | 142 | 1.70e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049878.t1 | AT2G44130 | 41.304 | 138 | 66 | 3 | 42 | 164 | 150 | 287 | 4.10e-28 | 108 |
| MS.gene049878.t1 | AT3G59940 | 39.161 | 143 | 67 | 3 | 42 | 164 | 152 | 294 | 2.16e-25 | 101 |
| MS.gene049878.t1 | AT1G80440 | 42.969 | 128 | 59 | 4 | 44 | 157 | 116 | 243 | 7.71e-23 | 93.6 |
| MS.gene049878.t1 | AT1G15670 | 33.659 | 205 | 88 | 7 | 1 | 157 | 43 | 247 | 2.79e-22 | 92.0 |
Find 0 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACACAGTAATATCGTAACT+CGG | + | 57404:5275-5294 | MS.gene049878:intergenic | 30.0% | |
| CTGATTAATTGACAGAACAA+CGG | + | 57404:5358-5377 | MS.gene049878:intergenic | 30.0% | |
| CTTATATCAACACATGATCT+GGG | + | 57404:5809-5828 | MS.gene049878:intergenic | 30.0% | |
| TACTCTCACAGAAAAAATAC+CGG | - | 57404:5163-5182 | MS.gene049878:CDS | 30.0% | |
| TGTTGATATAAGGGAACAAT+GGG | - | 57404:5817-5836 | MS.gene049878:CDS | 30.0% | |
| ! | CAGATCATGTGTTGATATAA+GGG | - | 57404:5808-5827 | MS.gene049878:CDS | 30.0% |
| !!! | ATCGTCCATTTATCGATTTT+CGG | + | 57404:5605-5624 | MS.gene049878:intergenic | 30.0% |
| AATCGATAAATGGACGATGT+TGG | - | 57404:5607-5626 | MS.gene049878:intron | 35.0% | |
| ATGACCCGAAAATCGATAAA+TGG | - | 57404:5597-5616 | MS.gene049878:CDS | 35.0% | |
| CCTTATATCAACACATGATC+TGG | + | 57404:5810-5829 | MS.gene049878:intergenic | 35.0% | |
| ! | AACCAAACAAGCAACTTTTC+TGG | + | 57404:5194-5213 | MS.gene049878:intergenic | 35.0% |
| ! | CCAGATCATGTGTTGATATA+AGG | - | 57404:5807-5826 | MS.gene049878:CDS | 35.0% |
| ! | GTGTTGATATAAGGGAACAA+TGG | - | 57404:5816-5835 | MS.gene049878:CDS | 35.0% |
| !! | TGTTTTTCGAATTCAGAGTG+AGG | + | 57404:5235-5254 | MS.gene049878:intergenic | 35.0% |
| !!! | TCGTCCATTTATCGATTTTC+GGG | + | 57404:5604-5623 | MS.gene049878:intergenic | 35.0% |
| AAACGTTCGTTCTTTGCTAC+CGG | - | 57404:5500-5519 | MS.gene049878:CDS | 40.0% | |
| ACAGTAATATCGTAACTCGG+TGG | + | 57404:5272-5291 | MS.gene049878:intergenic | 40.0% | |
| ACGATTTCCGAACGAATATG+TGG | - | 57404:5452-5471 | MS.gene049878:CDS | 40.0% | |
| AGAATGCGTTGAATACTGCT+TGG | - | 57404:5570-5589 | MS.gene049878:CDS | 40.0% | |
| CACCAGAAAAGTTGCTTGTT+TGG | - | 57404:5189-5208 | MS.gene049878:CDS | 40.0% | |
| CGATATTACTGTGTTTGACC+CGG | - | 57404:5280-5299 | MS.gene049878:CDS | 40.0% | |
| CGATTTCCGAACGAATATGT+GGG | - | 57404:5453-5472 | MS.gene049878:CDS | 40.0% | |
| GAATGCGTTGAATACTGCTT+GGG | - | 57404:5571-5590 | MS.gene049878:CDS | 40.0% | |
| GTCAATTAATCAGCTGCGAA+GGG | - | 57404:5365-5384 | MS.gene049878:CDS | 40.0% | |
| TGTCAATTAATCAGCTGCGA+AGG | - | 57404:5364-5383 | MS.gene049878:CDS | 40.0% | |
| ! | AGAGTGAGGTTGTTGTTCAT+GGG | + | 57404:5221-5240 | MS.gene049878:intergenic | 40.0% |
| ! | AGGGATGTTTGATGATTCTG+CGG | - | 57404:5718-5737 | MS.gene049878:intron | 40.0% |
| AACGTTCGTTCTTTGCTACC+GGG | - | 57404:5501-5520 | MS.gene049878:CDS | 45.0% | |
| AGTTGAAGGAGTTTGGGAAG+CGG | - | 57404:5775-5794 | MS.gene049878:CDS | 45.0% | |
| GAAATCGTAAACGAACACCG+CGG | + | 57404:5440-5459 | MS.gene049878:intergenic | 45.0% | |
| GAGGAGAGTTGAAGGAGTTT+GGG | - | 57404:5769-5788 | MS.gene049878:CDS | 45.0% | |
| GCAAAGAACGAACGTTTCTC+CGG | + | 57404:5497-5516 | MS.gene049878:intergenic | 45.0% | |
| TAAGAGGTTCGTAACTCGCT+GGG | + | 57404:5417-5436 | MS.gene049878:intergenic | 45.0% | |
| TATCAGGAACCGGATCAACT+CGG | + | 57404:5322-5341 | MS.gene049878:intergenic | 45.0% | |
| TTGACAGAACAACGGTAACC+CGG | + | 57404:5350-5369 | MS.gene049878:intergenic | 45.0% | |
| TTTCCGAACGAATATGTGGG+CGG | - | 57404:5456-5475 | MS.gene049878:CDS | 45.0% | |
| ! | CAGAGTGAGGTTGTTGTTCA+TGG | + | 57404:5222-5241 | MS.gene049878:intergenic | 45.0% |
| ! | GATGTTTGATGATTCTGCGG+AGG | - | 57404:5721-5740 | MS.gene049878:intron | 45.0% |
| ! | TAACTCGGTGGAGTTGAACT+GGG | + | 57404:5260-5279 | MS.gene049878:intergenic | 45.0% |
| !! | AGCAACTTTTCTGGTGTGAC+CGG | + | 57404:5185-5204 | MS.gene049878:intergenic | 45.0% |
| AACGAATATGTGGGCGGAGA+GGG | - | 57404:5462-5481 | MS.gene049878:CDS | 50.0% | |
| AAGCACGAGACGAGTGTGAA+GGG | - | 57404:5642-5661 | MS.gene049878:intron | 50.0% | |
| GAGTTACGAACCTCTTACCG+CGG | - | 57404:5420-5439 | MS.gene049878:CDS | 50.0% | |
| GGAGGAGAGTTGAAGGAGTT+TGG | - | 57404:5768-5787 | MS.gene049878:CDS | 50.0% | |
| GGTGGTTAGTGGTTATGCGA+CGG | - | 57404:5688-5707 | MS.gene049878:intron | 50.0% | |
| GTAAGAGGTTCGTAACTCGC+TGG | + | 57404:5418-5437 | MS.gene049878:intergenic | 50.0% | |
| GTTATGCGACGGAGAGTCAA+GGG | - | 57404:5699-5718 | MS.gene049878:intron | 50.0% | |
| GTTGAAGGAGTTTGGGAAGC+GGG | - | 57404:5776-5795 | MS.gene049878:CDS | 50.0% | |
| TAAACGAACACCGCGGTAAG+AGG | + | 57404:5433-5452 | MS.gene049878:intergenic | 50.0% | |
| TCCGGTTCCTGATATCCATC+CGG | - | 57404:5328-5347 | MS.gene049878:CDS | 50.0% | |
| TCGTCTCGTGCTTGACTCAT+CGG | + | 57404:5635-5654 | MS.gene049878:intergenic | 50.0% | |
| TCTCCGCCCACATATTCGTT+CGG | + | 57404:5462-5481 | MS.gene049878:intergenic | 50.0% | |
| TGACCCGGAGAGTTTGTCAT+GGG | - | 57404:5295-5314 | MS.gene049878:CDS | 50.0% | |
| TTCGTTCTTTGCTACCGGGT+CGG | - | 57404:5505-5524 | MS.gene049878:intron | 50.0% | |
| TTGACCCGGAGAGTTTGTCA+TGG | - | 57404:5294-5313 | MS.gene049878:CDS | 50.0% | |
| ! | GTAACTCGGTGGAGTTGAAC+TGG | + | 57404:5261-5280 | MS.gene049878:intergenic | 50.0% |
| ! | TGATGATTCTGCGGAGGTGT+TGG | - | 57404:5727-5746 | MS.gene049878:CDS | 50.0% |
| !! | CTGCGGAGGTGTTGGATATT+GGG | - | 57404:5735-5754 | MS.gene049878:CDS | 50.0% |
| !! | GAGGTGTTGGATATTGGGTC+GGG | - | 57404:5740-5759 | MS.gene049878:CDS | 50.0% |
| !! | GATCGGGTTTTTGTTGTGCG+TGG | - | 57404:5533-5552 | MS.gene049878:intron | 50.0% |
| !! | GGAGGTGTTGGATATTGGGT+CGG | - | 57404:5739-5758 | MS.gene049878:CDS | 50.0% |
| !! | TCTGCGGAGGTGTTGGATAT+TGG | - | 57404:5734-5753 | MS.gene049878:CDS | 50.0% |
| AAGGAGTTTGGGAAGCGGGT+CGG | - | 57404:5780-5799 | MS.gene049878:CDS | 55.0% | |
| AATATGTGGGCGGAGAGGGA+AGG | - | 57404:5466-5485 | MS.gene049878:CDS | 55.0% | |
| ACGAGACGAGTGTGAAGGGA+CGG | - | 57404:5646-5665 | MS.gene049878:intron | 55.0% | |
| ATATTGGGTCGGGTCAGTGG+AGG | - | 57404:5750-5769 | MS.gene049878:CDS | 55.0% | |
| CAAGCACGAGACGAGTGTGA+AGG | - | 57404:5641-5660 | MS.gene049878:intron | 55.0% | |
| CAGAACAACGGTAACCCGGA+TGG | + | 57404:5346-5365 | MS.gene049878:intergenic | 55.0% | |
| CCCGGATGGATATCAGGAAC+CGG | + | 57404:5332-5351 | MS.gene049878:intergenic | 55.0% | |
| CCGGTTCCTGATATCCATCC+GGG | - | 57404:5329-5348 | MS.gene049878:CDS | 55.0% | |
| CGGTAACCCGGATGGATATC+AGG | + | 57404:5338-5357 | MS.gene049878:intergenic | 55.0% | |
| CGGTCCCATGACAAACTCTC+CGG | + | 57404:5302-5321 | MS.gene049878:intergenic | 55.0% | |
| GAACGAATATGTGGGCGGAG+AGG | - | 57404:5461-5480 | MS.gene049878:CDS | 55.0% | |
| GAAGGGACGCTCGTGGTAAT+GGG | - | 57404:5382-5401 | MS.gene049878:CDS | 55.0% | |
| GAGTGTGAAGGGACGGTTGT+CGG | - | 57404:5653-5672 | MS.gene049878:intron | 55.0% | |
| GGTCAGTGGAGGAGAGTTGA+AGG | - | 57404:5761-5780 | MS.gene049878:CDS | 55.0% | |
| GGTCCCATGACAAACTCTCC+GGG | + | 57404:5301-5320 | MS.gene049878:intergenic | 55.0% | |
| GGTTATGCGACGGAGAGTCA+AGG | - | 57404:5698-5717 | MS.gene049878:intron | 55.0% | |
| TCGTTCTTTGCTACCGGGTC+GGG | - | 57404:5506-5525 | MS.gene049878:intron | 55.0% | |
| TGGATATTGGGTCGGGTCAG+TGG | - | 57404:5747-5766 | MS.gene049878:CDS | 55.0% | |
| !! | GTCATGGGACCGAGTTGATC+CGG | - | 57404:5310-5329 | MS.gene049878:CDS | 55.0% |
| !!! | GGCGAGTTTTGGGTGGTTAG+TGG | - | 57404:5677-5696 | MS.gene049878:intron | 55.0% |
| AACCCGATCGTAACCCGACC+CGG | + | 57404:5522-5541 | MS.gene049878:intergenic | 60.0% | |
| CGAAGGGACGCTCGTGGTAA+TGG | - | 57404:5381-5400 | MS.gene049878:CDS | 60.0% | |
| CTACCGGGTCGGGTTACGAT+CGG | - | 57404:5516-5535 | MS.gene049878:intron | 60.0% | |
| GCGGAGAGGGAAGGATATGC+CGG | - | 57404:5475-5494 | MS.gene049878:CDS | 60.0% | |
| TACCGGGTCGGGTTACGATC+GGG | - | 57404:5517-5536 | MS.gene049878:intron | 60.0% | |
| TGTGAAGGGACGGTTGTCGG+CGG | - | 57404:5656-5675 | MS.gene049878:intron | 60.0% | |
| ! | GGTTGTCGGCGGCGAGTTTT+GGG | - | 57404:5667-5686 | MS.gene049878:intron | 60.0% |
| !! | GCTCGTGGTAATGGGTGGGT+GGG | - | 57404:5390-5409 | MS.gene049878:CDS | 60.0% |
| !! | GGACGCTCGTGGTAATGGGT+GGG | - | 57404:5386-5405 | MS.gene049878:CDS | 60.0% |
| ! | CGGTTGTCGGCGGCGAGTTT+TGG | - | 57404:5666-5685 | MS.gene049878:intron | 65.0% |
| !! | CGCTCGTGGTAATGGGTGGG+TGG | - | 57404:5389-5408 | MS.gene049878:CDS | 65.0% |
| !! | GGGACGCTCGTGGTAATGGG+TGG | - | 57404:5385-5404 | MS.gene049878:CDS | 65.0% |
| !!! | TGTCGGCGGCGAGTTTTGGG+TGG | - | 57404:5670-5689 | MS.gene049878:intron | 65.0% |
| CAGCTGCGAAGGGACGCTCG+TGG | - | 57404:5375-5394 | MS.gene049878:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57404 | gene | 5160 | 5842 | 5160 | ID=MS.gene049878 |
| 57404 | mRNA | 5160 | 5842 | 5160 | ID=MS.gene049878.t1;Parent=MS.gene049878 |
| 57404 | exon | 5727 | 5842 | 5727 | ID=MS.gene049878.t1.exon1;Parent=MS.gene049878.t1 |
| 57404 | CDS | 5727 | 5842 | 5727 | ID=cds.MS.gene049878.t1;Parent=MS.gene049878.t1 |
| 57404 | exon | 5559 | 5624 | 5559 | ID=MS.gene049878.t1.exon2;Parent=MS.gene049878.t1 |
| 57404 | CDS | 5559 | 5624 | 5559 | ID=cds.MS.gene049878.t1;Parent=MS.gene049878.t1 |
| 57404 | exon | 5160 | 5521 | 5160 | ID=MS.gene049878.t1.exon3;Parent=MS.gene049878.t1 |
| 57404 | CDS | 5160 | 5521 | 5160 | ID=cds.MS.gene049878.t1;Parent=MS.gene049878.t1 |
| Gene Sequence |
| Protein sequence |
