Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23305.t1 | XP_003606962.1 | 90.7 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 1.90E-93 | 352.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23305.t1 | G7JHE9 | 90.7 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 1.4e-93 | 352.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049404 | MS.gene23305 | 0.804216 | 2.40E-49 | -1.69E-46 |
| MS.gene049878 | MS.gene23305 | 0.800179 | 1.63E-48 | -1.69E-46 |
| MS.gene050303 | MS.gene23305 | 0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene050680 | MS.gene23305 | 0.826532 | 2.58E-54 | -1.69E-46 |
| MS.gene050750 | MS.gene23305 | 0.810236 | 1.27E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene23305 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene23305 | 0.818654 | 1.75E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene23305 | 0.812291 | 4.57E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene23305 | 0.822523 | 2.26E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene23305 | 0.808942 | 2.42E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene23305 | 0.806438 | 8.23E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene23305 | 0.828104 | 1.08E-54 | -1.69E-46 |
| MS.gene052867 | MS.gene23305 | 0.818179 | 2.24E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene23305 | 0.818416 | 1.98E-52 | -1.69E-46 |
| MS.gene053953 | MS.gene23305 | 0.806191 | 9.27E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene23305 | 0.803031 | 4.23E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene23305 | 0.811823 | 5.77E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene23305 | 0.827834 | 1.26E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene23305 | 0.80785 | 4.13E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene23305 | 0.818473 | 1.93E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene23305 | 0.824557 | 7.57E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene23305 | 0.807544 | 4.80E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene23305 | 0.807376 | 5.21E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene23305 | 0.810944 | 8.96E-51 | -1.69E-46 |
| MS.gene057021 | MS.gene23305 | 0.809458 | 1.87E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene23305 | 0.847235 | 1.32E-59 | -1.69E-46 |
| MS.gene057555 | MS.gene23305 | 0.801039 | 1.09E-48 | -1.69E-46 |
| MS.gene057639 | MS.gene23305 | 0.838934 | 2.14E-57 | -1.69E-46 |
| MS.gene059128 | MS.gene23305 | 0.821353 | 4.22E-53 | -1.69E-46 |
| MS.gene059317 | MS.gene23305 | 0.81046 | 1.14E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene23305 | 0.809763 | 1.61E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene23305 | -0.823227 | 1.55E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene23305 | -0.813809 | 2.12E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23305.t1 | MTR_4g070640 | 90.654 | 214 | 19 | 1 | 1 | 214 | 1 | 213 | 3.48e-125 | 352 |
| MS.gene23305.t1 | MTR_4g070660 | 74.779 | 226 | 44 | 4 | 1 | 214 | 1 | 225 | 4.83e-104 | 300 |
| MS.gene23305.t1 | MTR_4g070630 | 67.059 | 170 | 54 | 2 | 46 | 214 | 1 | 169 | 3.09e-72 | 217 |
| MS.gene23305.t1 | MTR_3g117050 | 39.286 | 252 | 95 | 12 | 9 | 210 | 10 | 253 | 2.00e-28 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23305.t1 | AT5G66440 | 28.333 | 240 | 137 | 6 | 1 | 209 | 1 | 236 | 9.88e-22 | 90.1 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACTCAAGTGAGTATTTA+AGG | 0.169279 | 4.3:-34511166 | MS.gene23305:CDS |
| AATCATCACGTTGTTTGTTC+AGG | 0.261006 | 4.3:-34511087 | MS.gene23305:CDS |
| TCATCGTGTTCAGGTTCTTT+TGG | 0.272939 | 4.3:+34511047 | None:intergenic |
| CACTTCAACGAGTCGTATTA+AGG | 0.283376 | 4.3:+34511244 | None:intergenic |
| TAGGATTGCGTTGGCTTTCT+CGG | 0.305380 | 4.3:+34511295 | None:intergenic |
| AAGAAACGACGTCGTGTATT+GGG | 0.312077 | 4.3:-34510867 | MS.gene23305:CDS |
| ACGTTGTTTGTTCAGGGTTC+TGG | 0.316731 | 4.3:-34511080 | MS.gene23305:CDS |
| TTGCGTTGGCTTTCTCGGCT+TGG | 0.357630 | 4.3:+34511300 | None:intergenic |
| TCGTAAATATCATCGTGTTC+AGG | 0.373649 | 4.3:+34511038 | None:intergenic |
| AGAGAATAATAAAAGATGAT+AGG | 0.379532 | 4.3:-34510977 | MS.gene23305:CDS |
| GAAGAAACGACGTCGTGTAT+TGG | 0.380853 | 4.3:-34510868 | MS.gene23305:CDS |
| AGGTGAAGAAGAGTTATAGA+AGG | 0.390915 | 4.3:-34510908 | MS.gene23305:CDS |
| CTTGAGTTTCTTACTACAAC+TGG | 0.398117 | 4.3:+34511179 | None:intergenic |
| TCCTACCGTTGAGGAGGAAA+TGG | 0.408695 | 4.3:-34510799 | MS.gene23305:CDS |
| ATCATCACGTTGTTTGTTCA+GGG | 0.409938 | 4.3:-34511086 | MS.gene23305:CDS |
| AATAAAAGATGATAGGATAA+TGG | 0.411065 | 4.3:-34510970 | MS.gene23305:CDS |
| AATGGTGAGGATTTCGTATC+CGG | 0.424985 | 4.3:-34510781 | MS.gene23305:CDS |
| AAACTCAAGTGAGTATTTAA+GGG | 0.440864 | 4.3:-34511165 | MS.gene23305:CDS |
| CGTTGGCTTTCTCGGCTTGG+AGG | 0.445821 | 4.3:+34511303 | None:intergenic |
| TCTCGGCTTGGAGGTTAAAA+CGG | 0.449727 | 4.3:+34511312 | None:intergenic |
| TGAGTATTTAAGGGACGTTT+CGG | 0.464899 | 4.3:-34511156 | MS.gene23305:CDS |
| TCGCTGCTCATTTCATCCTC+CGG | 0.494759 | 4.3:+34510762 | None:intergenic |
| TAAAACACCACAAGCTTCAA+AGG | 0.505796 | 4.3:-34511274 | MS.gene23305:CDS |
| AAGCAACAAAGAGGATTTGA+GGG | 0.514889 | 4.3:-34510705 | MS.gene23305:CDS |
| GCAAAAGAAAATGAAAAGAG+AGG | 0.527440 | 4.3:-34510928 | MS.gene23305:CDS |
| TTGTGACCCTTTGAAGCTTG+TGG | 0.528150 | 4.3:+34511267 | None:intergenic |
| TGAGTTTCGTCGCATTGTAG+AGG | 0.528471 | 4.3:-34510739 | MS.gene23305:CDS |
| AAAGCAACAAAGAGGATTTG+AGG | 0.536464 | 4.3:-34510706 | MS.gene23305:CDS |
| TTTATTGCAAAGCAACAAAG+AGG | 0.547495 | 4.3:-34510714 | MS.gene23305:CDS |
| AAAACACCACAAGCTTCAAA+GGG | 0.570187 | 4.3:-34511273 | MS.gene23305:CDS |
| GATGATAGGATAATGGAAGA+AGG | 0.570977 | 4.3:-34510963 | MS.gene23305:CDS |
| GATAGGATAATGGAAGAAGG+TGG | 0.576977 | 4.3:-34510960 | MS.gene23305:CDS |
| GGTGTCAGAGTGAGAACGAA+AGG | 0.592349 | 4.3:-34510842 | MS.gene23305:CDS |
| AGCTACTCCTACCGTTGAGG+AGG | 0.595046 | 4.3:-34510805 | MS.gene23305:CDS |
| ACCATTTCCTCCTCAACGGT+AGG | 0.634496 | 4.3:+34510798 | None:intergenic |
| AACGACGTCGTGTATTGGGA+AGG | 0.637926 | 4.3:-34510863 | MS.gene23305:CDS |
| CCTCACCATTTCCTCCTCAA+CGG | 0.646170 | 4.3:+34510794 | None:intergenic |
| GGTGAGGATTTCGTATCCGG+AGG | 0.659971 | 4.3:-34510778 | MS.gene23305:CDS |
| AGTAGCTACTCCTACCGTTG+AGG | 0.669377 | 4.3:-34510808 | MS.gene23305:CDS |
| CCGTTGAGGAGGAAATGGTG+AGG | 0.712743 | 4.3:-34510794 | MS.gene23305:CDS |
| AGCAACAAAGAGGATTTGAG+GGG | 0.729509 | 4.3:-34510704 | MS.gene23305:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAGATGATAGGATAA+TGG | - | chr4.3:34511054-34511073 | MS.gene23305:CDS | 20.0% |
| !! | AGAGAATAATAAAAGATGAT+AGG | - | chr4.3:34511047-34511066 | MS.gene23305:CDS | 20.0% |
| !! | CGATAACAAAAACAAAATAA+TGG | + | chr4.3:34510907-34510926 | None:intergenic | 20.0% |
| ! | AAACTCAAGTGAGTATTTAA+GGG | - | chr4.3:34510859-34510878 | MS.gene23305:CDS | 25.0% |
| !!! | TTGTTTTAGTCTTGATTTCA+AGG | - | chr4.3:34510807-34510826 | MS.gene23305:CDS | 25.0% |
| GAAACTCAAGTGAGTATTTA+AGG | - | chr4.3:34510858-34510877 | MS.gene23305:CDS | 30.0% | |
| GCAAAAGAAAATGAAAAGAG+AGG | - | chr4.3:34511096-34511115 | MS.gene23305:CDS | 30.0% | |
| TTTATTGCAAAGCAACAAAG+AGG | - | chr4.3:34511310-34511329 | MS.gene23305:CDS | 30.0% | |
| AAAACACCACAAGCTTCAAA+GGG | - | chr4.3:34510751-34510770 | MS.gene23305:CDS | 35.0% | |
| AAAGCAACAAAGAGGATTTG+AGG | - | chr4.3:34511318-34511337 | MS.gene23305:CDS | 35.0% | |
| AAGCAACAAAGAGGATTTGA+GGG | - | chr4.3:34511319-34511338 | MS.gene23305:CDS | 35.0% | |
| AATCATCACGTTGTTTGTTC+AGG | - | chr4.3:34510937-34510956 | MS.gene23305:CDS | 35.0% | |
| AGGTGAAGAAGAGTTATAGA+AGG | - | chr4.3:34511116-34511135 | MS.gene23305:CDS | 35.0% | |
| ATCATCACGTTGTTTGTTCA+GGG | - | chr4.3:34510938-34510957 | MS.gene23305:CDS | 35.0% | |
| CTTGAGTTTCTTACTACAAC+TGG | + | chr4.3:34510848-34510867 | None:intergenic | 35.0% | |
| TAAAACACCACAAGCTTCAA+AGG | - | chr4.3:34510750-34510769 | MS.gene23305:CDS | 35.0% | |
| TCGTAAATATCATCGTGTTC+AGG | + | chr4.3:34510989-34511008 | None:intergenic | 35.0% | |
| TGAGTATTTAAGGGACGTTT+CGG | - | chr4.3:34510868-34510887 | MS.gene23305:CDS | 35.0% | |
| !! | GATGATAGGATAATGGAAGA+AGG | - | chr4.3:34511061-34511080 | MS.gene23305:CDS | 35.0% |
| !! | TTTGAAGCTTGTGGTGTTTT+AGG | + | chr4.3:34510751-34510770 | None:intergenic | 35.0% |
| AAGAAACGACGTCGTGTATT+GGG | - | chr4.3:34511157-34511176 | MS.gene23305:CDS | 40.0% | |
| AATGGTGAGGATTTCGTATC+CGG | - | chr4.3:34511243-34511262 | MS.gene23305:CDS | 40.0% | |
| AGCAACAAAGAGGATTTGAG+GGG | - | chr4.3:34511320-34511339 | MS.gene23305:CDS | 40.0% | |
| CACTTCAACGAGTCGTATTA+AGG | + | chr4.3:34510783-34510802 | None:intergenic | 40.0% | |
| !! | GATAGGATAATGGAAGAAGG+TGG | - | chr4.3:34511064-34511083 | MS.gene23305:CDS | 40.0% |
| !! | TCATCGTGTTCAGGTTCTTT+TGG | + | chr4.3:34510980-34510999 | None:intergenic | 40.0% |
| ACGTTGTTTGTTCAGGGTTC+TGG | - | chr4.3:34510944-34510963 | MS.gene23305:CDS | 45.0% | |
| GAAGAAACGACGTCGTGTAT+TGG | - | chr4.3:34511156-34511175 | MS.gene23305:CDS | 45.0% | |
| TGAGTTTCGTCGCATTGTAG+AGG | - | chr4.3:34511285-34511304 | MS.gene23305:CDS | 45.0% | |
| !! | TAGGATTGCGTTGGCTTTCT+CGG | + | chr4.3:34510732-34510751 | None:intergenic | 45.0% |
| !! | TCTCGGCTTGGAGGTTAAAA+CGG | + | chr4.3:34510715-34510734 | None:intergenic | 45.0% |
| !! | TTGTGACCCTTTGAAGCTTG+TGG | + | chr4.3:34510760-34510779 | None:intergenic | 45.0% |
| !!! | GTGGTGTTTTAGGATTGCGT+TGG | + | chr4.3:34510741-34510760 | None:intergenic | 45.0% |
| AACGACGTCGTGTATTGGGA+AGG | - | chr4.3:34511161-34511180 | MS.gene23305:CDS | 50.0% | |
| ACCATTTCCTCCTCAACGGT+AGG | + | chr4.3:34511229-34511248 | None:intergenic | 50.0% | |
| AGTAGCTACTCCTACCGTTG+AGG | - | chr4.3:34511216-34511235 | MS.gene23305:CDS | 50.0% | |
| CCTCACCATTTCCTCCTCAA+CGG | + | chr4.3:34511233-34511252 | None:intergenic | 50.0% | |
| GGTGTCAGAGTGAGAACGAA+AGG | - | chr4.3:34511182-34511201 | MS.gene23305:CDS | 50.0% | |
| TCCTACCGTTGAGGAGGAAA+TGG | - | chr4.3:34511225-34511244 | MS.gene23305:CDS | 50.0% | |
| TCGCTGCTCATTTCATCCTC+CGG | + | chr4.3:34511265-34511284 | None:intergenic | 50.0% | |
| AGCTACTCCTACCGTTGAGG+AGG | - | chr4.3:34511219-34511238 | MS.gene23305:CDS | 55.0% | |
| CCGTTGAGGAGGAAATGGTG+AGG | - | chr4.3:34511230-34511249 | MS.gene23305:CDS | 55.0% | |
| GGTGAGGATTTCGTATCCGG+AGG | - | chr4.3:34511246-34511265 | MS.gene23305:CDS | 55.0% | |
| !! | TTGCGTTGGCTTTCTCGGCT+TGG | + | chr4.3:34510727-34510746 | None:intergenic | 55.0% |
| !! | CGTTGGCTTTCTCGGCTTGG+AGG | + | chr4.3:34510724-34510743 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 34510701 | 34511345 | 34510701 | ID=MS.gene23305 |
| chr4.3 | mRNA | 34510701 | 34511345 | 34510701 | ID=MS.gene23305.t1;Parent=MS.gene23305 |
| chr4.3 | exon | 34510701 | 34511345 | 34510701 | ID=MS.gene23305.t1.exon1;Parent=MS.gene23305.t1 |
| chr4.3 | CDS | 34510701 | 34511345 | 34510701 | ID=cds.MS.gene23305.t1;Parent=MS.gene23305.t1 |
| Gene Sequence |
| Protein sequence |
