AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64249


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64249.t1 MTR_1g046070 97.425 233 4 1 1 231 1 233 1.43e-155 431
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64249.t1 AT4G13540 46.061 165 71 4 49 212 37 184 1.10e-32 118
MS.gene64249.t1 AT3G23930 41.279 172 78 4 52 212 40 199 1.12e-23 95.1

Find 50 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAAGAGTGGGAATTAA+TGG 0.247721 1.3:-27658952 MS.gene64249:CDS
ACTTACCATCATATGTTCTT+TGG 0.249382 1.3:+27658817 None:intergenic
GAAGAAGTTGAAAATCAAAT+TGG 0.250274 1.3:-27657981 MS.gene64249:CDS
TTCTTCTCTTTAGTTTGAAT+TGG 0.254934 1.3:+27658001 None:intergenic
AAAGCTTTAAAAGCTCAATT+AGG 0.297525 1.3:-27658109 MS.gene64249:CDS
GATAATGGTGTTTGCTTTCA+AGG 0.301137 1.3:-27659099 MS.gene64249:CDS
ATTAATGGGGACTAAGCTTT+TGG 0.315398 1.3:-27658937 MS.gene64249:CDS
AAAGGGAATTTGACTTGTTA+AGG 0.331176 1.3:-27658057 MS.gene64249:CDS
TAAAGGAGATAATGTGTGAT+AGG 0.355573 1.3:-27659151 MS.gene64249:CDS
ACTTCTACAAGAAGAAATAA+AGG 0.374088 1.3:-27659168 MS.gene64249:CDS
GAACTATGAGAGGGAGATAA+TGG 0.387809 1.3:-27659114 MS.gene64249:CDS
GAGAAAGAGTGGGAATTAAT+GGG 0.405188 1.3:-27658951 MS.gene64249:CDS
GAGGGAAGTGGTGGAAGAGA+AGG 0.429388 1.3:-27659024 MS.gene64249:CDS
AAGAGAAGGAAGAGAAGATA+AGG 0.430927 1.3:-27659010 MS.gene64249:CDS
AAGAAGTTGAAAATCAAATT+GGG 0.435875 1.3:-27657980 MS.gene64249:CDS
AGTGAAGCAGTTGAGGGAAG+TGG 0.444912 1.3:-27659036 MS.gene64249:CDS
TCACAGGAGATGGTTTGTAT+TGG 0.451338 1.3:-27658201 MS.gene64249:intron
AGAAGAATTGTGAGAAAGAG+TGG 0.454742 1.3:-27658962 MS.gene64249:CDS
ATAAGGGAGATGGAAGAGGT+TGG 0.460472 1.3:-27658993 MS.gene64249:CDS
TTGCTTTCAAGGAGGCAGAT+TGG 0.464224 1.3:-27659088 MS.gene64249:CDS
AAGAAGAAGTGAAGCAGTTG+AGG 0.467138 1.3:-27659043 MS.gene64249:CDS
AAGAAAGGTACAAACAAGAA+AGG 0.469395 1.3:-27658075 MS.gene64249:CDS
AGAGAAGGAAGAGAAGATAA+GGG 0.476340 1.3:-27659009 MS.gene64249:CDS
AGAAGAAGTGAAGCAGTTGA+GGG 0.480459 1.3:-27659042 MS.gene64249:CDS
AAGAGAGTAGGAACTATGAG+AGG 0.487797 1.3:-27659124 MS.gene64249:CDS
GAAGATAAGGGAGATGGAAG+AGG 0.488344 1.3:-27658997 MS.gene64249:CDS
TACAAATTGAAAATCTGAAG+AGG 0.492525 1.3:-27658159 MS.gene64249:CDS
GTGATAGGGAGAAAGAGAGT+AGG 0.501313 1.3:-27659136 MS.gene64249:CDS
GGAAGAGAAGATAAGGGAGA+TGG 0.503969 1.3:-27659003 MS.gene64249:CDS
TCTTGCAATTAAGAATGAGT+TGG 0.505081 1.3:-27658850 MS.gene64249:CDS
GAGATGAAGCTGTTGAGAAG+TGG 0.508742 1.3:-27658884 MS.gene64249:CDS
TAGGTTCAGTGGAGAAAGAA+AGG 0.512086 1.3:-27658090 MS.gene64249:CDS
GAAGCAGTTGAGGGAAGTGG+TGG 0.513840 1.3:-27659033 MS.gene64249:CDS
AAAAGCTCAATTAGGTTCAG+TGG 0.518823 1.3:-27658101 MS.gene64249:CDS
AGAGAGTAGGAACTATGAGA+GGG 0.522297 1.3:-27659123 MS.gene64249:CDS
CTCATCCAAAGAACATATGA+TGG 0.522947 1.3:-27658822 MS.gene64249:intron
AAAGGAGATAATGTGTGATA+GGG 0.526845 1.3:-27659150 MS.gene64249:CDS
AGAAAGGTACAAACAAGAAA+GGG 0.530283 1.3:-27658074 MS.gene64249:CDS
GGAGATGGAAGAGGTTGGAA+TGG 0.546010 1.3:-27658988 MS.gene64249:CDS
ACTTGTTAAGGCAAAGCTTG+AGG 0.554263 1.3:-27658045 MS.gene64249:CDS
ATTGGAAACAAGAGATGAAG+AGG 0.564436 1.3:-27659070 MS.gene64249:CDS
CAAAGCTTGAGGATCATGAA+TGG 0.565818 1.3:-27658034 MS.gene64249:CDS
ACAAATTGAAAATCTGAAGA+GGG 0.598118 1.3:-27658158 MS.gene64249:CDS
GAAGAATTGTGAGAAAGAGT+GGG 0.599083 1.3:-27658961 MS.gene64249:CDS
AGAAAGAGTGGGAATTAATG+GGG 0.618319 1.3:-27658950 MS.gene64249:CDS
AGATGAAGGAGGAAAGAGCA+AGG 0.618929 1.3:-27658908 MS.gene64249:CDS
TGGTTTGTATTGGAAAGCTG+AGG 0.636251 1.3:-27658191 MS.gene64249:CDS
TTTGGTTCAAGAGATGAAGG+AGG 0.645416 1.3:-27658919 MS.gene64249:CDS
AATGGTGTTTGCTTTCAAGG+AGG 0.717432 1.3:-27659096 MS.gene64249:CDS
GGGGCCAATTCAAAGATGGG+GGG 0.731569 1.3:-27659266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAAATAAATTTTTTT+TGG + chr1.3:27658696-27658715 None:intergenic 0.0%
!!! AAATTCATTACATATTTTTG+TGG + chr1.3:27658021-27658040 None:intergenic 15.0%
!! AAATACAAACACTAATTGTT+TGG + chr1.3:27658781-27658800 None:intergenic 20.0%
!! AAGAAGTTGAAAATCAAATT+GGG - chr1.3:27659244-27659263 MS.gene64249:CDS 20.0%
!! ATTAATATGTGACAATAATG+TGG - chr1.3:27658904-27658923 MS.gene64249:CDS 20.0%
!!! AATTTTGAAAAGTACTATGA+TGG + chr1.3:27658879-27658898 None:intergenic 20.0%
!!! ATTTTGAAAAGTACTATGAT+GGG + chr1.3:27658878-27658897 None:intergenic 20.0%
! ACAAATTGAAAATCTGAAGA+GGG - chr1.3:27659066-27659085 MS.gene64249:CDS 25.0%
! ACTTCTACAAGAAGAAATAA+AGG - chr1.3:27658056-27658075 MS.gene64249:CDS 25.0%
! GAAGAAGTTGAAAATCAAAT+TGG - chr1.3:27659243-27659262 MS.gene64249:CDS 25.0%
! TACAAATTGAAAATCTGAAG+AGG - chr1.3:27659065-27659084 MS.gene64249:CDS 25.0%
!!! AAAGCTTTAAAAGCTCAATT+AGG - chr1.3:27659115-27659134 MS.gene64249:CDS 25.0%
!!! TTCTTCTCTTTAGTTTGAAT+TGG + chr1.3:27659226-27659245 None:intergenic 25.0%
AAAGGAGATAATGTGTGATA+GGG - chr1.3:27658074-27658093 MS.gene64249:CDS 30.0%
ACTTACCATCATATGTTCTT+TGG + chr1.3:27658410-27658429 None:intergenic 30.0%
TAAAGGAGATAATGTGTGAT+AGG - chr1.3:27658073-27658092 MS.gene64249:CDS 30.0%
TCTTGCAATTAAGAATGAGT+TGG - chr1.3:27658374-27658393 MS.gene64249:intron 30.0%
! AAGAAAGGTACAAACAAGAA+AGG - chr1.3:27659149-27659168 MS.gene64249:CDS 30.0%
! AGAAAGGTACAAACAAGAAA+GGG - chr1.3:27659150-27659169 MS.gene64249:CDS 30.0%
! TACTTGAGCTTATTTGCTTT+TGG - chr1.3:27658992-27659011 MS.gene64249:CDS 30.0%
!! AAAGGGAATTTGACTTGTTA+AGG - chr1.3:27659167-27659186 MS.gene64249:CDS 30.0%
AAAAGCTCAATTAGGTTCAG+TGG - chr1.3:27659123-27659142 MS.gene64249:CDS 35.0%
AAGAGAAGGAAGAGAAGATA+AGG - chr1.3:27658214-27658233 MS.gene64249:intron 35.0%
AGAAAGAGTGGGAATTAATG+GGG - chr1.3:27658274-27658293 MS.gene64249:intron 35.0%
AGAAGAATTGTGAGAAAGAG+TGG - chr1.3:27658262-27658281 MS.gene64249:intron 35.0%
AGAGAAGGAAGAGAAGATAA+GGG - chr1.3:27658215-27658234 MS.gene64249:intron 35.0%
ATTGGAAACAAGAGATGAAG+AGG - chr1.3:27658154-27658173 MS.gene64249:CDS 35.0%
CTCATCCAAAGAACATATGA+TGG - chr1.3:27658402-27658421 MS.gene64249:intron 35.0%
GAAGAATTGTGAGAAAGAGT+GGG - chr1.3:27658263-27658282 MS.gene64249:intron 35.0%
GAGAAAGAGTGGGAATTAAT+GGG - chr1.3:27658273-27658292 MS.gene64249:intron 35.0%
TGAGAAAGAGTGGGAATTAA+TGG - chr1.3:27658272-27658291 MS.gene64249:intron 35.0%
! ATTAATGGGGACTAAGCTTT+TGG - chr1.3:27658287-27658306 MS.gene64249:intron 35.0%
! GATAATGGTGTTTGCTTTCA+AGG - chr1.3:27658125-27658144 MS.gene64249:CDS 35.0%
!! GCTTTTGGAAATATACTCAC+AGG - chr1.3:27659007-27659026 MS.gene64249:CDS 35.0%
AAAAGTAGTGTGTGGCAGAT+GGG - chr1.3:27658845-27658864 MS.gene64249:CDS 40.0%
AAGAAGAAGTGAAGCAGTTG+AGG - chr1.3:27658181-27658200 MS.gene64249:CDS 40.0%
AAGAGAGTAGGAACTATGAG+AGG - chr1.3:27658100-27658119 MS.gene64249:CDS 40.0%
AATGGTGTTTGCTTTCAAGG+AGG - chr1.3:27658128-27658147 MS.gene64249:CDS 40.0%
AGAAGAAGTGAAGCAGTTGA+GGG - chr1.3:27658182-27658201 MS.gene64249:CDS 40.0%
AGAGAGTAGGAACTATGAGA+GGG - chr1.3:27658101-27658120 MS.gene64249:CDS 40.0%
CAAAGCTTGAGGATCATGAA+TGG - chr1.3:27659190-27659209 MS.gene64249:CDS 40.0%
GAACTATGAGAGGGAGATAA+TGG - chr1.3:27658110-27658129 MS.gene64249:CDS 40.0%
GGAAATATACTCACAGGAGA+TGG - chr1.3:27659013-27659032 MS.gene64249:CDS 40.0%
TAGGTTCAGTGGAGAAAGAA+AGG - chr1.3:27659134-27659153 MS.gene64249:CDS 40.0%
! ACTTGTTAAGGCAAAGCTTG+AGG - chr1.3:27659179-27659198 MS.gene64249:CDS 40.0%
! TCACAGGAGATGGTTTGTAT+TGG - chr1.3:27659023-27659042 MS.gene64249:CDS 40.0%
! TGGTTTGTATTGGAAAGCTG+AGG - chr1.3:27659033-27659052 MS.gene64249:CDS 40.0%
! TTTGGTTCAAGAGATGAAGG+AGG - chr1.3:27658305-27658324 MS.gene64249:intron 40.0%
!! GCCACACACTACTTTTCAAA+GGG + chr1.3:27658841-27658860 None:intergenic 40.0%
!! TGCCACACACTACTTTTCAA+AGG + chr1.3:27658842-27658861 None:intergenic 40.0%
!!! GCTTTTGGTTCAAGAGATGA+AGG - chr1.3:27658302-27658321 MS.gene64249:intron 40.0%
AAAGTAGTGTGTGGCAGATG+GGG - chr1.3:27658846-27658865 MS.gene64249:CDS 45.0%
AGATGAAGGAGGAAAGAGCA+AGG - chr1.3:27658316-27658335 MS.gene64249:intron 45.0%
ATAAGGGAGATGGAAGAGGT+TGG - chr1.3:27658231-27658250 MS.gene64249:intron 45.0%
GAAAAGTAGTGTGTGGCAGA+TGG - chr1.3:27658844-27658863 MS.gene64249:CDS 45.0%
GAAGATAAGGGAGATGGAAG+AGG - chr1.3:27658227-27658246 MS.gene64249:intron 45.0%
GAGATGAAGCTGTTGAGAAG+TGG - chr1.3:27658340-27658359 MS.gene64249:intron 45.0%
GGAAGAGAAGATAAGGGAGA+TGG - chr1.3:27658221-27658240 MS.gene64249:intron 45.0%
GTGATAGGGAGAAAGAGAGT+AGG - chr1.3:27658088-27658107 MS.gene64249:CDS 45.0%
TTGCTTTCAAGGAGGCAGAT+TGG - chr1.3:27658136-27658155 MS.gene64249:CDS 45.0%
! GCCCTTTGAAAAGTAGTGTG+TGG - chr1.3:27658837-27658856 MS.gene64249:CDS 45.0%
AGTGAAGCAGTTGAGGGAAG+TGG - chr1.3:27658188-27658207 MS.gene64249:CDS 50.0%
GGAGATGGAAGAGGTTGGAA+TGG - chr1.3:27658236-27658255 MS.gene64249:intron 50.0%
GAAGCAGTTGAGGGAAGTGG+TGG - chr1.3:27658191-27658210 MS.gene64249:CDS 55.0%
! GAGGGAAGTGGTGGAAGAGA+AGG - chr1.3:27658200-27658219 MS.gene64249:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 27657973 27659273 27657973 ID=MS.gene64249
chr1.3 mRNA 27657973 27659273 27657973 ID=MS.gene64249.t1;Parent=MS.gene64249
chr1.3 exon 27658823 27659273 27658823 ID=MS.gene64249.t1.exon1;Parent=MS.gene64249.t1
chr1.3 CDS 27658823 27659273 27658823 ID=cds.MS.gene64249.t1;Parent=MS.gene64249.t1
chr1.3 exon 27657973 27658217 27657973 ID=MS.gene64249.t1.exon2;Parent=MS.gene64249.t1
chr1.3 CDS 27657973 27658217 27657973 ID=cds.MS.gene64249.t1;Parent=MS.gene64249.t1
Gene Sequence

>MS.gene64249

ATGGGGGGAAGTGTTAGTGTTAGCAAGAAAAATGAGATAAAGTATCCACAAAAATATGTAATGAATTTGAAAGAGAAAGTGAGACTTCTACAAGAAGAAATAAAGGAGATAATGTGTGATAGGGAGAAAGAGAGTAGGAACTATGAGAGGGAGATAATGGTGTTTGCTTTCAAGGAGGCAGATTGGAAACAAGAGATGAAGAGGTTGAAAGAAGAAGTGAAGCAGTTGAGGGAAGTGGTGGAAGAGAAGGAAGAGAAGATAAGGGAGATGGAAGAGGTTGGAATGGTTGAGAAGAATTGTGAGAAAGAGTGGGAATTAATGGGGACTAAGCTTTTGGTTCAAGAGATGAAGGAGGAAAGAGCAAGGAGAGATGAAGCTGTTGAGAAGTGGAAACAACTTTATCTTGCAATTAAGAATGAGTTGGATGATCTCATCCAAAGAACATATGATGGTAAGTTAATCATAATTTGTTATTGATGTCTTAAGACATGTTTGAATTGAGTTATTTTATCTTATTTATGAGCATGTTTGAGAGTTTATAAAAACAAATTATTATGTGTATAATCTATCTATTTTTATCTTATAAGTTGAATTGAGTTATTTTAGCTTATTTATGAGTATGTTTGAGAGAGTTTATAAAAAACAAATTATAATGTGTGTAATCTATTTTTATCTTATAAGTTTTCTAAAATAGTCTATAAAAACAGTTTATAGTTTATGCCAAAAAAAATTTATTTAATTTTTTTTTGTAGAAATAACTTATAAAATAATGATTATATAATAATTGTTTAATTAAGATTTTTATCCAAACAATTAGTGTTTGTATTTTTTTGTGATTTGCATTAGTCTTTGTATGCTATTAATGCCCTTTGAAAAGTAGTGTGTGGCAGATGGGGTTCAATCCCATCATAGTACTTTTCAAAATTAGTCAATTAATATGTGACAATAATGTGGTGAATTATTTTTATTATCTTCTTGAGAAATATTCATACTACTATGCATGAATGCTATGTTTGAACTACTTGAGCTTATTTGCTTTTGGAAATATACTCACAGGAGATGGTTTGTATTGGAAAGCTGAGGAAAATGACATACAAATTGAAAATCTGAAGAGGGAATTGCAAGAGAAAGAAGAAAGCATAAAAGCTTTAAAAGCTCAATTAGGTTCAGTGGAGAAAGAAAGGTACAAACAAGAAAGGGAATTTGACTTGTTAAGGCAAAGCTTGAGGATCATGAATGGCAAGAAGAATCCAATTCAAACTAAAGAGAAGAAGAAGTTGAAAATCAAATTGGGAAAATAA

Protein sequence

>MS.gene64249.t1

MGGSVSVSKKNEIKYPQKYVMNLKEKVRLLQEEIKEIMCDREKESRNYEREIMVFAFKEADWKQEMKRLKEEVKQLREVVEEKEEKIREMEEVGMVEKNCEKEWELMGTKLLVQEMKEERARRDEAVEKWKQLYLAIKNELDDLIQRTYDGDGLYWKAEENDIQIENLKRELQEKEESIKALKAQLGSVEKERYKQEREFDLLRQSLRIMNGKKNPIQTKEKKKLKIKLGK