AlfalfaGEDB | MS.gene050284

M. sativa cultivar XinJiangDaYe / MS.gene050284

KEGG: -
GO: -
PFAM: PF04520.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050284.t1	XP_013450120.1	95.9	98	4	0	1	98	120	217	1.30E-36	162.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050284.t1	A0A072U4S8	95.9	98	4	0	1	98	120	217	9.1e-37	162.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050284	MS.gene050287	0.893421	6.39E-75	-1.69E-46
MS.gene050284	MS.gene051752	0.800802	1.21E-48	-1.69E-46
MS.gene050284	MS.gene24710	0.895518	8.88E-76	-1.69E-46
MS.gene050284	MS.gene33483	-0.813763	2.17E-51	-1.69E-46
MS.gene050284	MS.gene41198	0.801233	9.92E-49	-1.69E-46
MS.gene050284	MS.gene54090	-0.836279	1.03E-56	-1.69E-46
MS.gene050284	MS.gene62887	0.825424	4.72E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050284.t1	MTR_7g105130	98.980	98	1	0	1	98	120	217	6.65e-65	195
MS.gene050284.t1	MTR_2g086660	47.917	96	49	1	4	98	160	255	5.93e-22	86.7
MS.gene050284.t1	MTR_2g086660	47.312	93	48	1	4	95	160	252	4.30e-20	82.0
MS.gene050284.t1	MTR_4g132020	53.012	83	32	3	14	96	99	174	6.68e-19	77.0
MS.gene050284.t1	MTR_2g023390	58.000	50	21	0	49	98	107	156	2.89e-15	67.0
MS.gene050284.t1	MTR_3g052330	36.522	115	46	3	11	98	84	198	7.45e-15	67.0
MS.gene050284.t1	MTR_7g101250	61.224	49	15	1	50	98	116	160	7.26e-13	60.8
MS.gene050284.t1	MTR_2g094410	61.224	49	15	1	50	98	112	156	1.24e-12	60.1
MS.gene050284.t1	MTR_2g018175	54.000	50	21	1	49	98	79	126	2.64e-12	58.5
MS.gene050284.t1	MTR_4g059320	60.417	48	15	1	49	96	114	157	6.06e-12	58.5
MS.gene050284.t1	MTR_2g023070	47.727	88	41	2	9	96	134	216	1.02e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050284.t1	AT3G15040	54.444	90	39	1	11	98	154	243	8.21e-21	83.2
MS.gene050284.t1	AT4G21970	43.182	88	49	1	12	98	58	145	2.69e-19	77.4
MS.gene050284.t1	AT3G45210	56.667	60	22	1	39	98	93	148	7.27e-17	70.9
MS.gene050284.t1	AT4G04630	50.000	92	41	2	12	98	77	168	3.85e-16	69.7
MS.gene050284.t1	AT1G29640	45.455	88	37	3	11	98	63	139	2.37e-15	67.0
MS.gene050284.t1	AT5G60680	52.857	70	29	1	29	98	98	163	1.62e-11	57.4
MS.gene050284.t1	AT5G03230	49.057	53	23	1	46	98	118	166	1.73e-11	57.4

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCGACTCGGCGGCATAAT+CGG	0.409285	7.3:-9475479	None:intergenic
TTCAATCAAGAAAACCGGTT+TGG	0.414262	7.3:-9475697	None:intergenic
ACCGGTTCAATCAAGAAAAC+CGG	0.431244	7.3:-9475702	None:intergenic
CCTTCACCGGAGCAGATTGT+TGG	0.453692	7.3:-9475454	None:intergenic
TGTTGATGAGCTTGCGGTGT+TGG	0.456949	7.3:+9475517	MS.gene050284:CDS
GGCATAATCGGAACCTTCAC+CGG	0.480745	7.3:-9475467	None:intergenic
GAGATTGTTGCGAGAGGCTC+TGG	0.482407	7.3:+9475581	MS.gene050284:CDS
AACGCTGTTCTGCGCCAAAC+CGG	0.488010	7.3:+9475683	MS.gene050284:CDS
CATCAACATCCCTACGCCTC+GGG	0.490643	7.3:-9475502	None:intergenic
AAAACCGAAAAGGTAGTTCT+CGG	0.517462	7.3:-9475611	None:intergenic
AACTCCTTCCAAAACCGAAA+AGG	0.517512	7.3:-9475621	None:intergenic
CAAAGGGAGAGATCTTCGTC+AGG	0.523089	7.3:+9475655	MS.gene050284:CDS
CCAACAATCTGCTCCGGTGA+AGG	0.523875	7.3:+9475454	MS.gene050284:CDS
ATTCTTCCAACAATCTGCTC+CGG	0.527245	7.3:+9475448	MS.gene050284:CDS
CGAGTCGATTCCCGAGGCGT+AGG	0.528775	7.3:+9475492	MS.gene050284:CDS
GAGTCGATTCCCGAGGCGTA+GGG	0.543346	7.3:+9475493	MS.gene050284:CDS
TCATCAACATCCCTACGCCT+CGG	0.557335	7.3:-9475503	None:intergenic
CTACGCCTCGGGAATCGACT+CGG	0.583023	7.3:-9475491	None:intergenic
GGAGTTGGAAGAACTCTCAA+AGG	0.586119	7.3:+9475638	MS.gene050284:CDS
TGATGATGACGACGACGGCA+AGG	0.596482	7.3:+9475541	MS.gene050284:CDS
GAGTTGGAAGAACTCTCAAA+GGG	0.607793	7.3:+9475639	MS.gene050284:CDS
CCTCATGAGATTGTTGCGAG+AGG	0.640232	7.3:+9475575	MS.gene050284:CDS
CCTCTCGCAACAATCTCATG+AGG	0.649415	7.3:-9475575	None:intergenic
TGCCGCCGAGTCGATTCCCG+AGG	0.656419	7.3:+9475486	MS.gene050284:CDS
TAGGGATGTTGATGAGCTTG+CGG	0.658353	7.3:+9475511	MS.gene050284:CDS
CTCGCAACAATCTCATGAGG+CGG	0.658511	7.3:-9475572	None:intergenic
CGCCTCGGGAATCGACTCGG+CGG	0.734091	7.3:-9475488	None:intergenic
TTGGATGATGATGACGACGA+CGG	0.773393	7.3:+9475536	MS.gene050284:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTTGGAAGAATTTTCTAGT+AGG	-	chr7.3:9475440-9475459	None:intergenic	30.0%
	AAAACCGAAAAGGTAGTTCT+CGG	-	chr7.3:9475614-9475633	None:intergenic	35.0%
!!	GAAGAATTTTCTAGTAGGCA+TGG	-	chr7.3:9475435-9475454	None:intergenic	35.0%
	AACTCCTTCCAAAACCGAAA+AGG	-	chr7.3:9475624-9475643	None:intergenic	40.0%
	ATTCTTCCAACAATCTGCTC+CGG	+	chr7.3:9475448-9475467	MS.gene050284:CDS	40.0%
	GAGTTGGAAGAACTCTCAAA+GGG	+	chr7.3:9475639-9475658	MS.gene050284:CDS	40.0%
!	GAGAACTACCTTTTCGGTTT+TGG	+	chr7.3:9475613-9475632	MS.gene050284:CDS	40.0%
!!!	ACTACCTTTTCGGTTTTGGA+AGG	+	chr7.3:9475617-9475636	MS.gene050284:CDS	40.0%
!!!	TTTTCGGTTTTGGAAGGAGT+TGG	+	chr7.3:9475623-9475642	MS.gene050284:CDS	40.0%
	GGAGTTGGAAGAACTCTCAA+AGG	+	chr7.3:9475638-9475657	MS.gene050284:CDS	45.0%
!	TAGGGATGTTGATGAGCTTG+CGG	+	chr7.3:9475511-9475530	MS.gene050284:CDS	45.0%
!	TTCGCCGAGAACTACCTTTT+CGG	+	chr7.3:9475607-9475626	MS.gene050284:CDS	45.0%
!	TTGGATGATGATGACGACGA+CGG	+	chr7.3:9475536-9475555	MS.gene050284:CDS	45.0%
	CAAAGGGAGAGATCTTCGTC+AGG	+	chr7.3:9475655-9475674	MS.gene050284:CDS	50.0%
	CCTCATGAGATTGTTGCGAG+AGG	+	chr7.3:9475575-9475594	MS.gene050284:CDS	50.0%
	CCTCTCGCAACAATCTCATG+AGG	-	chr7.3:9475578-9475597	None:intergenic	50.0%
	CTCGCAACAATCTCATGAGG+CGG	-	chr7.3:9475575-9475594	None:intergenic	50.0%
	GGCATAATCGGAACCTTCAC+CGG	-	chr7.3:9475470-9475489	None:intergenic	50.0%
	TCATCAACATCCCTACGCCT+CGG	-	chr7.3:9475506-9475525	None:intergenic	50.0%
!	TGTTGATGAGCTTGCGGTGT+TGG	+	chr7.3:9475517-9475536	MS.gene050284:CDS	50.0%
!!	AATCGACTCGGCGGCATAAT+CGG	-	chr7.3:9475482-9475501	None:intergenic	50.0%
	AACGCTGTTCTGCGCCAAAC+CGG	+	chr7.3:9475683-9475702	MS.gene050284:CDS	55.0%
	CATCAACATCCCTACGCCTC+GGG	-	chr7.3:9475505-9475524	None:intergenic	55.0%
	CCAACAATCTGCTCCGGTGA+AGG	+	chr7.3:9475454-9475473	MS.gene050284:CDS	55.0%
	CCTTCACCGGAGCAGATTGT+TGG	-	chr7.3:9475457-9475476	None:intergenic	55.0%
	GAGATTGTTGCGAGAGGCTC+TGG	+	chr7.3:9475581-9475600	MS.gene050284:CDS	55.0%
!	TGATGATGACGACGACGGCA+AGG	+	chr7.3:9475541-9475560	MS.gene050284:CDS	55.0%
	CTACGCCTCGGGAATCGACT+CGG	-	chr7.3:9475494-9475513	None:intergenic	60.0%
!	GAGTCGATTCCCGAGGCGTA+GGG	+	chr7.3:9475493-9475512	MS.gene050284:CDS	60.0%
!	CGAGTCGATTCCCGAGGCGT+AGG	+	chr7.3:9475492-9475511	MS.gene050284:CDS	65.0%
!	TGCCGCCGAGTCGATTCCCG+AGG	+	chr7.3:9475486-9475505	MS.gene050284:CDS	70.0%
!!	CGCCTCGGGAATCGACTCGG+CGG	-	chr7.3:9475491-9475510	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	9475422	9475718	9475422	ID=MS.gene050284
chr7.3	mRNA	9475422	9475718	9475422	ID=MS.gene050284.t1;Parent=MS.gene050284
chr7.3	exon	9475422	9475718	9475422	ID=MS.gene050284.t1.exon1;Parent=MS.gene050284.t1
chr7.3	CDS	9475422	9475718	9475422	ID=cds.MS.gene050284.t1;Parent=MS.gene050284.t1

Gene Sequence	>MS.gene050284 ATGATTCAATCCATGCCTACTAGAAAATTCTTCCAACAATCTGCTCCGGTGAAGGTTCCGATTATGCCGCCGAGTCGATTCCCGAGGCGTAGGGATGTTGATGAGCTTGCGGTGTTGGATGATGATGACGACGACGGCAAGGAGTTGTTGCCGCCTCATGAGATTGTTGCGAGAGGCTCTGGTGTTTCGCCGAGAACTACCTTTTCGGTTTTGGAAGGAGTTGGAAGAACTCTCAAAGGGAGAGATCTTCGTCAGGTTCGAAACGCTGTTCTGCGCCAAACCGGTTTTCTTGATTGA
Protein sequence	>MS.gene050284.t1 MIQSMPTRKFFQQSAPVKVPIMPPSRFPRRRDVDELAVLDDDDDDGKELLPPHEIVARGSGVSPRTTFSVLEGVGRTLKGRDLRQVRNAVLRQTGFLD