AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052790.t1 MTR_4g014580 51.376 218 42 5 1 160 515 726 1.82e-55 187
MS.gene052790.t1 MTR_4g014990 81.731 104 19 0 70 173 995 1098 2.71e-51 176
MS.gene052790.t1 MTR_4g014570 73.786 103 25 1 70 172 1037 1137 6.40e-46 160
MS.gene052790.t1 MTR_4g015030 74.038 104 24 1 70 173 1016 1116 2.38e-45 159
MS.gene052790.t1 MTR_6g015680 45.455 154 78 2 16 168 955 1103 8.98e-34 126
MS.gene052790.t1 MTR_4g015060 63.725 102 36 1 70 171 1009 1109 4.91e-32 121
MS.gene052790.t1 MTR_3g104130 50.000 102 51 0 70 171 74 175 3.90e-30 108
MS.gene052790.t1 MTR_3g104210 50.000 102 51 0 70 171 74 175 3.90e-30 108
MS.gene052790.t1 MTR_6g015405 65.823 79 27 0 70 148 1002 1080 6.61e-30 115
MS.gene052790.t1 MTR_6g015505 49.219 128 39 3 70 171 777 904 2.33e-29 113
MS.gene052790.t1 MTR_3g104170 49.020 102 52 0 70 171 74 175 5.02e-29 105
MS.gene052790.t1 MTR_3g104240 49.020 102 52 0 70 171 74 175 5.02e-29 105
MS.gene052790.t1 MTR_6g015595 48.462 130 39 4 70 171 75 204 2.46e-27 102
MS.gene052790.t1 MTR_6g015455 60.000 90 35 1 70 158 1344 1433 1.20e-26 105
MS.gene052790.t1 MTR_6g015455 53.846 91 37 1 70 160 360 445 7.90e-21 89.0
MS.gene052790.t1 MTR_7g091540 63.750 80 28 1 70 148 609 688 8.12e-26 103
MS.gene052790.t1 MTR_6g015490 51.515 99 48 0 70 168 820 918 7.96e-25 100
MS.gene052790.t1 MTR_6g015490 51.515 99 48 0 70 168 1046 1144 8.33e-25 100
MS.gene052790.t1 MTR_6g015575 50.000 100 48 1 57 154 36 135 2.01e-24 94.4
MS.gene052790.t1 MTR_0294s0010 51.042 96 47 0 70 165 794 889 2.68e-24 99.0
MS.gene052790.t1 MTR_6g016000 58.750 80 32 1 70 148 1045 1124 2.72e-24 99.0
MS.gene052790.t1 MTR_6g016000 58.750 80 32 1 70 148 1027 1106 3.14e-24 99.0
MS.gene052790.t1 MTR_4g023400 55.128 78 34 1 70 146 1469 1546 3.87e-24 98.6
MS.gene052790.t1 MTR_6g015510 50.505 99 38 1 70 168 992 1079 9.41e-24 97.4
MS.gene052790.t1 MTR_4g415060 67.089 79 25 1 93 171 1 78 3.63e-23 87.8
MS.gene052790.t1 MTR_6g015765 49.495 99 39 2 70 168 74 161 3.95e-23 90.5
MS.gene052790.t1 MTR_6g015450 55.000 80 35 1 70 148 54 133 7.53e-23 88.6
MS.gene052790.t1 MTR_6g015775 39.610 154 76 3 16 168 1018 1155 1.21e-22 94.4
MS.gene052790.t1 MTR_6g015730 52.525 99 32 2 70 168 1277 1360 6.52e-22 92.0
MS.gene052790.t1 MTR_6g015745 55.405 74 33 0 70 143 994 1067 1.80e-21 90.9
MS.gene052790.t1 MTR_6g015430 44.167 120 62 3 20 136 962 1079 2.56e-21 90.5
MS.gene052790.t1 MTR_6g015430 44.167 120 62 3 20 136 769 886 3.57e-21 90.1
MS.gene052790.t1 MTR_4g020760 41.121 107 52 1 70 165 570 676 2.90e-20 87.4
MS.gene052790.t1 MTR_4g020590 47.674 86 34 1 70 144 1453 1538 3.83e-20 87.0
MS.gene052790.t1 MTR_4g020590 47.674 86 34 1 70 144 1460 1545 3.83e-20 87.0
MS.gene052790.t1 MTR_6g015435 43.902 123 43 2 47 168 954 1051 6.12e-20 86.7
MS.gene052790.t1 MTR_7g055970 50.633 79 38 1 70 147 432 510 3.97e-19 84.0
MS.gene052790.t1 MTR_6g015665 75.472 53 13 0 70 122 1054 1106 5.05e-19 84.0
MS.gene052790.t1 MTR_4g021057 46.753 77 40 1 70 145 1492 1568 1.04e-18 82.8
MS.gene052790.t1 MTR_0286s0020 38.947 95 58 0 70 164 474 568 2.57e-18 81.6
MS.gene052790.t1 MTR_4g020850 44.872 78 42 1 70 146 1677 1754 3.12e-18 81.6
MS.gene052790.t1 MTR_4g068200 55.263 76 33 1 70 144 545 620 5.22e-18 80.9
MS.gene052790.t1 MTR_6g015695 59.375 64 26 0 70 133 1004 1067 9.16e-18 80.1
MS.gene052790.t1 MTR_4g017740 43.902 82 45 1 70 150 40 121 1.35e-17 76.6
MS.gene052790.t1 MTR_4g020550 42.857 77 44 0 70 146 1428 1504 1.57e-17 79.7
MS.gene052790.t1 MTR_4g020700 41.758 91 42 1 70 149 1472 1562 1.62e-17 79.7
MS.gene052790.t1 MTR_6g015600 67.308 52 16 1 70 121 695 745 1.64e-17 79.3
MS.gene052790.t1 MTR_4g020700 41.758 91 42 1 70 149 1472 1562 1.81e-17 79.3
MS.gene052790.t1 MTR_4g020640 43.038 79 45 0 69 147 1487 1565 2.68e-17 79.0
MS.gene052790.t1 MTR_4g020640 43.038 79 45 0 69 147 1487 1565 2.76e-17 79.0
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1475 1544 4.17e-17 78.2
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1942 2011 4.49e-17 78.2
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1474 1543 4.59e-17 78.2
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1976 2045 4.72e-17 78.2
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1474 1543 4.72e-17 78.2
MS.gene052790.t1 MTR_7g088950 57.143 70 29 1 70 138 1440 1509 4.95e-17 78.2
MS.gene052790.t1 MTR_7g088950 54.930 71 31 1 70 139 1976 2046 5.72e-17 78.2
MS.gene052790.t1 MTR_7g088950 54.930 71 31 1 70 139 1440 1510 5.89e-17 77.8
MS.gene052790.t1 MTR_7g088950 54.930 71 31 1 70 139 1440 1510 6.42e-17 77.8
MS.gene052790.t1 MTR_8g037220 60.000 55 22 0 70 124 660 714 2.74e-16 75.9
MS.gene052790.t1 MTR_8g037220 60.000 55 22 0 70 124 881 935 2.81e-16 75.9
MS.gene052790.t1 MTR_4g021170 44.872 78 42 1 70 146 1454 1531 3.37e-16 75.9
MS.gene052790.t1 MTR_4g020535 48.438 64 33 0 70 133 941 1004 3.39e-16 75.9
MS.gene052790.t1 MTR_6g015410 55.882 68 30 0 70 137 967 1034 1.22e-15 73.9
MS.gene052790.t1 MTR_6g015675 61.111 54 21 0 70 123 416 469 1.82e-15 73.6
MS.gene052790.t1 MTR_4g020490 40.244 82 48 1 70 150 1598 1679 3.69e-15 72.8
MS.gene052790.t1 MTR_4g021023 40.698 86 40 1 70 144 1225 1310 3.90e-15 72.8
MS.gene052790.t1 MTR_4g021030 43.902 82 45 1 70 150 40 121 3.92e-15 68.9
MS.gene052790.t1 MTR_4g021023 40.698 86 40 1 70 144 1476 1561 4.39e-15 72.4
MS.gene052790.t1 MTR_4g021027 52.703 74 16 1 1 55 407 480 9.82e-15 71.2
MS.gene052790.t1 MTR_6g015580 61.538 52 19 1 70 121 816 866 1.10e-14 71.2
MS.gene052790.t1 MTR_6g015580 61.538 52 19 1 70 121 982 1032 1.19e-14 71.2
MS.gene052790.t1 MTR_8g037235 52.727 55 26 0 70 124 868 922 7.35e-14 68.9
MS.gene052790.t1 MTR_8g011870 55.769 52 23 0 69 120 185 236 1.18e-13 67.8
MS.gene052790.t1 MTR_6g465280 46.575 73 38 1 47 118 6 78 1.89e-13 63.5
MS.gene052790.t1 MTR_5g036240 50.909 55 27 0 70 124 804 858 5.71e-13 66.2
MS.gene052790.t1 MTR_2g437560 52.830 53 25 0 70 122 927 979 9.19e-13 65.9
MS.gene052790.t1 MTR_8g011850 47.273 55 29 0 70 124 982 1036 1.76e-12 65.1
MS.gene052790.t1 MTR_3g072140 43.750 64 36 0 70 133 774 837 3.31e-12 64.3
MS.gene052790.t1 MTR_8g012080 46.429 56 30 0 70 125 950 1005 3.54e-12 63.9
MS.gene052790.t1 MTR_8g012200 52.830 53 25 0 70 122 958 1010 3.57e-12 63.9
MS.gene052790.t1 MTR_5g047480 48.214 56 29 0 70 125 952 1007 3.68e-12 63.9
MS.gene052790.t1 MTR_8g012190 48.214 56 29 0 70 125 941 996 1.28e-11 62.4
MS.gene052790.t1 MTR_8g012190 48.214 56 29 0 70 125 947 1002 1.36e-11 62.4
MS.gene052790.t1 MTR_8g020430 46.154 78 19 1 1 55 636 713 1.46e-11 62.4
MS.gene052790.t1 MTR_8g020430 57.447 47 20 0 70 116 927 973 3.30e-11 61.2
MS.gene052790.t1 MTR_8g012005 49.057 53 27 0 70 122 86 138 1.67e-11 61.2
MS.gene052790.t1 MTR_5g047530 46.429 56 30 0 70 125 952 1007 1.74e-11 62.0
MS.gene052790.t1 MTR_0468s0010 49.057 53 27 0 70 122 922 974 2.42e-11 61.6
MS.gene052790.t1 MTR_0552s0040 41.333 75 37 1 70 144 401 468 5.06e-11 60.5
MS.gene052790.t1 MTR_8g073730 47.170 53 28 0 70 122 951 1003 6.15e-11 60.5
MS.gene052790.t1 MTR_4g021095 41.333 75 37 1 70 144 1341 1408 6.67e-11 60.5
MS.gene052790.t1 MTR_8g011950 47.273 55 29 0 70 124 930 984 7.07e-11 60.5
MS.gene052790.t1 MTR_8g011710 50.943 53 26 0 70 122 973 1025 9.06e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTATCATGAGACCTTTA+TGG 0.161546 4.4:-86227387 None:intergenic
TGAACCAGTTGGCGAAAATT+TGG 0.190447 4.4:+86228831 MS.gene052790:CDS
GAACCAGTTGGCGAAAATTT+GGG 0.281243 4.4:+86228832 MS.gene052790:CDS
GCAGAGAGTAGTATCAAATA+TGG 0.372012 4.4:+86228726 MS.gene052790:CDS
CCAACAAGAACATTCTTATC+TGG 0.387749 4.4:-86228868 None:intergenic
CCAGATAAGAATGTTCTTGT+TGG 0.394164 4.4:+86228868 MS.gene052790:CDS
GGAAGAGGACTTGGAACAAA+TGG 0.409764 4.4:+86227508 MS.gene052790:intron
TATGGAGATGTCCAGTGGTA+TGG 0.442238 4.4:-86227369 None:intergenic
TAACAAATTGGAAGAGGACT+TGG 0.456569 4.4:+86227499 MS.gene052790:intron
GTTCGCCACCTTTGAAAATG+AGG 0.482686 4.4:+86228699 MS.gene052790:CDS
ACCACTGGACATCTCCATAA+AGG 0.482951 4.4:+86227373 MS.gene052790:CDS
ATGGAGATGTCCAGTGGTAT+GGG 0.504712 4.4:-86227368 None:intergenic
GATCACTCTTCAAGTAGAGC+TGG 0.504877 4.4:+86228924 MS.gene052790:CDS
AACCAGTTGGCGAAAATTTG+GGG 0.551858 4.4:+86228833 MS.gene052790:CDS
TGAAGAGTGATCCTCTTGGC+AGG 0.553675 4.4:-86228913 None:intergenic
TCTGAGGTTTCCCATACCAC+TGG 0.561143 4.4:+86227358 MS.gene052790:CDS
CTCATATATGATGAACCAGT+TGG 0.562231 4.4:+86228820 MS.gene052790:CDS
TGGGAAACCTCAGACAGCCT+TGG 0.562909 4.4:-86227349 None:intergenic
AAGAATGTTCTTGTTGGTGA+TGG 0.577141 4.4:+86228874 MS.gene052790:CDS
TAATACTCAAAGATTGTCCA+AGG 0.597568 4.4:+86227332 MS.gene052790:CDS
AGATTGTCCAAGGCTGTCTG+AGG 0.609340 4.4:+86227342 MS.gene052790:CDS
TACTTGAAGAGTGATCCTCT+TGG 0.627194 4.4:-86228917 None:intergenic
CATTCAGCTCTATAAGAGAG+AGG 0.638242 4.4:+86228675 MS.gene052790:CDS
GTAGTATCAAATATGGAACC+TGG 0.639177 4.4:+86228733 MS.gene052790:CDS
ACCTTTATGGAGATGTCCAG+TGG 0.643406 4.4:-86227374 None:intergenic
ACAATCTCGACTTTGTCACC+AGG 0.643713 4.4:-86228751 None:intergenic
CCACCTTTGAAAATGAGGAG+TGG 0.645361 4.4:+86228704 MS.gene052790:CDS
AGTGTCATCTGCAATCAAAG+TGG 0.650441 4.4:-86227540 None:intergenic
AAAATGAATGTCCTGCCAAG+AGG 0.655746 4.4:+86228902 MS.gene052790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATGATTATGTTATG+AGG + chr4.4:86228347-86228366 MS.gene052790:intron 15.0%
!! CTGTTTAATTATTAACAAAT+TGG + chr4.4:86227487-86227506 MS.gene052790:intron 15.0%
!! AATTATTAACAAATTGGAAG+AGG + chr4.4:86227493-86227512 MS.gene052790:intron 20.0%
!! TCTTTATTAATTGAATGTTG+TGG + chr4.4:86227938-86227957 MS.gene052790:intron 20.0%
! AAATGATTATGTTATGAGGA+AGG + chr4.4:86228351-86228370 MS.gene052790:intron 25.0%
! AATTTATCATGAGACCTTTA+TGG - chr4.4:86227390-86227409 None:intergenic 25.0%
! AATTTGGATAATTATCACCA+GGG - chr4.4:86228493-86228512 None:intergenic 25.0%
! CTAACAAGGTAAAAAACAAT+TGG + chr4.4:86227587-86227606 MS.gene052790:intron 25.0%
! TTTAAGTGCAAAGAATTCTA+AGG + chr4.4:86227868-86227887 MS.gene052790:intron 25.0%
!! TTTTACCTTGTTAGTGAAAA+GGG - chr4.4:86227581-86227600 None:intergenic 25.0%
!! TTTTTACCTTGTTAGTGAAA+AGG - chr4.4:86227582-86227601 None:intergenic 25.0%
!!! AGTATTAGCTTTTCAAGATT+AGG - chr4.4:86227319-86227338 None:intergenic 25.0%
AAGTATTTCCTTCGATCATT+CGG + chr4.4:86227648-86227667 MS.gene052790:intron 30.0%
ATTGGATATATTTCCTTGTG+TGG + chr4.4:86227605-86227624 MS.gene052790:intron 30.0%
CAATTTGGATAATTATCACC+AGG - chr4.4:86228494-86228513 None:intergenic 30.0%
TAACAAATATATCGACAGCT+TGG - chr4.4:86228029-86228048 None:intergenic 30.0%
TAATACTCAAAGATTGTCCA+AGG + chr4.4:86227332-86227351 MS.gene052790:CDS 30.0%
TTATAGTTCCTCAAGTGAAA+GGG + chr4.4:86228549-86228568 MS.gene052790:intron 30.0%
TTTATAGTTCCTCAAGTGAA+AGG + chr4.4:86228548-86228567 MS.gene052790:intron 30.0%
! CACTTAAAGTCTTATAGTTC+TGG + chr4.4:86228102-86228121 MS.gene052790:intron 30.0%
! TAATTGAAGGCTAGTCAATT+TGG - chr4.4:86228509-86228528 None:intergenic 30.0%
! TGCAAAGAATTCTAAGGAAT+TGG + chr4.4:86227874-86227893 MS.gene052790:intron 30.0%
!!! TCGAGATTGTTGTTGTTTTT+GGG + chr4.4:86228764-86228783 MS.gene052790:CDS 30.0%
AAAGACTAAGAACCTTTCCA+AGG - chr4.4:86227796-86227815 None:intergenic 35.0%
AAATCCTTTATAGCCACACA+AGG - chr4.4:86227621-86227640 None:intergenic 35.0%
AGAAGAACCGTCATAATTGA+AGG - chr4.4:86228522-86228541 None:intergenic 35.0%
AGGTCTCTTCTAATTCGAAT+GGG + chr4.4:86227994-86228013 MS.gene052790:intron 35.0%
ATTCTGTAAACCGTGAAAGT+AGG - chr4.4:86228438-86228457 None:intergenic 35.0%
ATTTCCTTGTGTGGCTATAA+AGG + chr4.4:86227614-86227633 MS.gene052790:intron 35.0%
CAGAAACTAAAAAATCCCTG+AGG + chr4.4:86227974-86227993 MS.gene052790:intron 35.0%
CAGAGATTTCAACGTATACA+TGG - chr4.4:86227449-86227468 None:intergenic 35.0%
CCAACAAGAACATTCTTATC+TGG - chr4.4:86228871-86228890 None:intergenic 35.0%
CCAGATAAGAATGTTCTTGT+TGG + chr4.4:86228868-86228887 MS.gene052790:CDS 35.0%
CTCATATATGATGAACCAGT+TGG + chr4.4:86228820-86228839 MS.gene052790:CDS 35.0%
CTTCATTTCTTTGGGTACTA+CGG + chr4.4:86228225-86228244 MS.gene052790:intron 35.0%
GCAGAGAGTAGTATCAAATA+TGG + chr4.4:86228726-86228745 MS.gene052790:CDS 35.0%
GCAGATAACATAACATCAGA+TGG + chr4.4:86228614-86228633 MS.gene052790:intron 35.0%
GTAGTATCAAATATGGAACC+TGG + chr4.4:86228733-86228752 MS.gene052790:CDS 35.0%
TAACAAATTGGAAGAGGACT+TGG + chr4.4:86227499-86227518 MS.gene052790:intron 35.0%
TAGTACCCAAAGAAATGAAG+TGG - chr4.4:86228225-86228244 None:intergenic 35.0%
TTCAACGTATACATGGTTCT+TGG - chr4.4:86227442-86227461 None:intergenic 35.0%
! AAGAATGTTCTTGTTGGTGA+TGG + chr4.4:86228874-86228893 MS.gene052790:CDS 35.0%
! ACTTTCACGAGATGCAATTT+TGG + chr4.4:86227841-86227860 MS.gene052790:intron 35.0%
! AGATGGACTCAAAAATGTGT+TGG + chr4.4:86228631-86228650 MS.gene052790:intron 35.0%
! CAATTTTGAGCCTACTTTCA+CGG + chr4.4:86228425-86228444 MS.gene052790:intron 35.0%
!! TGACTAGCCTTCAATTATGA+CGG + chr4.4:86228512-86228531 MS.gene052790:intron 35.0%
!! TGGAAAGGTTCTTAGTCTTT+TGG + chr4.4:86227796-86227815 MS.gene052790:intron 35.0%
!!! GTCGAGATTGTTGTTGTTTT+TGG + chr4.4:86228763-86228782 MS.gene052790:CDS 35.0%
AAAATGAATGTCCTGCCAAG+AGG + chr4.4:86228902-86228921 MS.gene052790:CDS 40.0%
AACCAGTTGGCGAAAATTTG+GGG + chr4.4:86228833-86228852 MS.gene052790:CDS 40.0%
AAGTTATGCCCTTTCACTTG+AGG - chr4.4:86228560-86228579 None:intergenic 40.0%
AATACGACTGTCAAGTCATG+TGG + chr4.4:86228455-86228474 MS.gene052790:intron 40.0%
ACGTATACATGGTTCTTGGA+AGG - chr4.4:86227438-86227457 None:intergenic 40.0%
ACTGGACCACTTCATTTCTT+TGG + chr4.4:86228216-86228235 MS.gene052790:intron 40.0%
AGTGTCATCTGCAATCAAAG+TGG - chr4.4:86227543-86227562 None:intergenic 40.0%
CATTCAGCTCTATAAGAGAG+AGG + chr4.4:86228675-86228694 MS.gene052790:CDS 40.0%
CTACTACACCACAATACACT+CGG - chr4.4:86228169-86228188 None:intergenic 40.0%
CTCGGCTCTTCACATATAAT+TGG - chr4.4:86228151-86228170 None:intergenic 40.0%
CTGGACCACTTCATTTCTTT+GGG + chr4.4:86228217-86228236 MS.gene052790:intron 40.0%
GAACCAGTTGGCGAAAATTT+GGG + chr4.4:86228832-86228851 MS.gene052790:CDS 40.0%
GAGGTCTCTTCTAATTCGAA+TGG + chr4.4:86227993-86228012 MS.gene052790:intron 40.0%
GGAAATAGATGACAGACAGT+GGG - chr4.4:86227768-86227787 None:intergenic 40.0%
GTGTATTGTGGTGTAGTAGT+TGG + chr4.4:86228170-86228189 MS.gene052790:intron 40.0%
TACATGGTTCTTGGAAGGTT+AGG - chr4.4:86227433-86227452 None:intergenic 40.0%
TACTTGAAGAGTGATCCTCT+TGG - chr4.4:86228920-86228939 None:intergenic 40.0%
TCGAATTAGAAGAGACCTCA+GGG - chr4.4:86227992-86228011 None:intergenic 40.0%
TGAACCAGTTGGCGAAAATT+TGG + chr4.4:86228831-86228850 MS.gene052790:CDS 40.0%
TTCGAATTAGAAGAGACCTC+AGG - chr4.4:86227993-86228012 None:intergenic 40.0%
! AAGTGCCCTTTTCACTAACA+AGG + chr4.4:86227573-86227592 MS.gene052790:CDS 40.0%
! CTCTGAGAACTCTTATTCTG+TGG + chr4.4:86227464-86227483 MS.gene052790:intron 40.0%
!! CTTAGTCTTTTGGTAGAGTG+CGG + chr4.4:86227806-86227825 MS.gene052790:intron 40.0%
AAATGAAGTGGTCCAGTAGC+CGG - chr4.4:86228213-86228232 None:intergenic 45.0%
AAGAACCTTTCCAAGGACCT+CGG - chr4.4:86227789-86227808 None:intergenic 45.0%
ACAATCTCGACTTTGTCACC+AGG - chr4.4:86228754-86228773 None:intergenic 45.0%
ACCACTGGACATCTCCATAA+AGG + chr4.4:86227373-86227392 MS.gene052790:CDS 45.0%
ACCTTTATGGAGATGTCCAG+TGG - chr4.4:86227377-86227396 None:intergenic 45.0%
ATAAGAGATGACATGCCAGC+AGG - chr4.4:86227719-86227738 None:intergenic 45.0%
ATCCAAGACCGAATGATCGA+AGG - chr4.4:86227659-86227678 None:intergenic 45.0%
ATGGAGATGTCCAGTGGTAT+GGG - chr4.4:86227371-86227390 None:intergenic 45.0%
CAGTGGGAACTACTTCTTGA+TGG - chr4.4:86227752-86227771 None:intergenic 45.0%
CGGAAATAGATGACAGACAG+TGG - chr4.4:86227769-86227788 None:intergenic 45.0%
CTGTCTGTCATCTATTTCCG+AGG + chr4.4:86227769-86227788 MS.gene052790:intron 45.0%
GATCACTCTTCAAGTAGAGC+TGG + chr4.4:86228924-86228943 MS.gene052790:CDS 45.0%
GGAAGAGGACTTGGAACAAA+TGG + chr4.4:86227508-86227527 MS.gene052790:intron 45.0%
TATGGAGATGTCCAGTGGTA+TGG - chr4.4:86227372-86227391 None:intergenic 45.0%
TATTTCCGAGGTCCTTGGAA+AGG + chr4.4:86227781-86227800 MS.gene052790:intron 45.0%
TCATCTATTTCCGAGGTCCT+TGG + chr4.4:86227776-86227795 MS.gene052790:intron 45.0%
TCCTTAGTTCAAACACCTGC+TGG + chr4.4:86227701-86227720 MS.gene052790:intron 45.0%
TGGTTCTTGGAAGGTTAGGA+AGG - chr4.4:86227429-86227448 None:intergenic 45.0%
TTCCTTCGATCATTCGGTCT+TGG + chr4.4:86227654-86227673 MS.gene052790:intron 45.0%
! CCACCTTTGAAAATGAGGAG+TGG + chr4.4:86228704-86228723 MS.gene052790:CDS 45.0%
! CCACTCCTCATTTTCAAAGG+TGG - chr4.4:86228707-86228726 None:intergenic 45.0%
! CTGCCACTCCTCATTTTCAA+AGG - chr4.4:86228710-86228729 None:intergenic 45.0%
! GTTCGCCACCTTTGAAAATG+AGG + chr4.4:86228699-86228718 MS.gene052790:CDS 45.0%
AACGACTCTGCTGCAATGGA+TGG - chr4.4:86228261-86228280 None:intergenic 50.0%
AATGAAGTGGTCCAGTAGCC+GGG - chr4.4:86228212-86228231 None:intergenic 50.0%
ACGACTGTCAAGTCATGTGG+TGG + chr4.4:86228458-86228477 MS.gene052790:intron 50.0%
AGATTGTCCAAGGCTGTCTG+AGG + chr4.4:86227342-86227361 MS.gene052790:CDS 50.0%
CATTGCAGCAGAGTCGTTTC+AGG + chr4.4:86228263-86228282 MS.gene052790:intron 50.0%
CTGAAACGACTCTGCTGCAA+TGG - chr4.4:86228265-86228284 None:intergenic 50.0%
GCCAGCAGGTGTTTGAACTA+AGG - chr4.4:86227705-86227724 None:intergenic 50.0%
GTGAAGAGCCGAGTGTATTG+TGG + chr4.4:86228158-86228177 MS.gene052790:intron 50.0%
TGAAGAGTGATCCTCTTGGC+AGG - chr4.4:86228916-86228935 None:intergenic 50.0%
! TCTGAGGTTTCCCATACCAC+TGG + chr4.4:86227358-86227377 MS.gene052790:CDS 50.0%
! TGCCCCAAATTTTCGCCAAC+TGG - chr4.4:86228838-86228857 None:intergenic 50.0%
AGCAGAGTCGTTTCAGGAGG+GGG + chr4.4:86228269-86228288 MS.gene052790:intron 55.0%
CAGCAGAGTCGTTTCAGGAG+GGG + chr4.4:86228268-86228287 MS.gene052790:intron 55.0%
CTGCTGCAATGGATGGCAGA+GGG - chr4.4:86228254-86228273 None:intergenic 55.0%
GCAGCAGAGTCGTTTCAGGA+GGG + chr4.4:86228267-86228286 MS.gene052790:intron 55.0%
TCTGCTGCAATGGATGGCAG+AGG - chr4.4:86228255-86228274 None:intergenic 55.0%
TGCAGCAGAGTCGTTTCAGG+AGG + chr4.4:86228266-86228285 MS.gene052790:intron 55.0%
TGCTGCAATGGATGGCAGAG+GGG - chr4.4:86228253-86228272 None:intergenic 55.0%
TGGGAAACCTCAGACAGCCT+TGG - chr4.4:86227352-86227371 None:intergenic 55.0%
TGTGGTGGTTTCTTGCTCCC+TGG + chr4.4:86228473-86228492 MS.gene052790:intron 55.0%
AAGTGGTCCAGTAGCCGGGA+AGG - chr4.4:86228208-86228227 None:intergenic 60.0%
AGTGGTCCAGTAGCCGGGAA+GGG - chr4.4:86228207-86228226 None:intergenic 60.0%
CAGAGTCGTTTCAGGAGGGG+GGG + chr4.4:86228271-86228290 MS.gene052790:intron 60.0%
GCAGAGTCGTTTCAGGAGGG+GGG + chr4.4:86228270-86228289 MS.gene052790:intron 60.0%
GCTGCAATGGATGGCAGAGG+GGG - chr4.4:86228252-86228271 None:intergenic 60.0%
GGCACTTCTAGTCCCCTTCC+CGG + chr4.4:86228191-86228210 MS.gene052790:intron 60.0%
CTAGTCCCCTTCCCGGCTAC+TGG + chr4.4:86228198-86228217 MS.gene052790:intron 65.0%
GTGGTCCAGTAGCCGGGAAG+GGG - chr4.4:86228206-86228225 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 86227313 86228990 86227313 ID=MS.gene052790
chr4.4 mRNA 86227313 86228990 86227313 ID=MS.gene052790.t1;Parent=MS.gene052790
chr4.4 exon 86227313 86227394 86227313 ID=MS.gene052790.t1.exon1;Parent=MS.gene052790.t1
chr4.4 CDS 86227313 86227394 86227313 ID=cds.MS.gene052790.t1;Parent=MS.gene052790.t1
chr4.4 exon 86227513 86227594 86227513 ID=MS.gene052790.t1.exon2;Parent=MS.gene052790.t1
chr4.4 CDS 86227513 86227594 86227513 ID=cds.MS.gene052790.t1;Parent=MS.gene052790.t1
chr4.4 exon 86228633 86228990 86228633 ID=MS.gene052790.t1.exon3;Parent=MS.gene052790.t1
chr4.4 CDS 86228633 86228990 86228633 ID=cds.MS.gene052790.t1;Parent=MS.gene052790.t1
Gene Sequence

>MS.gene052790

ATGCCTAATCTTGAAAAGCTAATACTCAAAGATTGTCCAAGGCTGTCTGAGGTTTCCCATACCACTGGACATCTCCATAAAGGTCTCATGATAAATTTGAAAGACTGTATTAGCCTTCCTAACCTTCCAAGAACCATGTATACGTTGAAATCTCTGAGAACTCTTATTCTGTGGCTGTTTAATTATTAACAAATTGGAAGAGGACTTGGAACAAATGGAATCTTTAACCACTTTGATTGCAGATGACACTGCAATAACAGAAGTGCCCTTTTCACTAACAAGGTAAAAAACAATTGGATATATTTCCTTGTGTGGCTATAAAGGATTTTCACATGAAGTATTTCCTTCGATCATTCGGTCTTGGATGTCACTAACAAATAATCTCTCATCCTTAGTTCAAACACCTGCTGGCATGTCATCTCTTATTTCTTTAGATCCATCAAGAAGTAGTTCCCACTGTCTGTCATCTATTTCCGAGGTCCTTGGAAAGGTTCTTAGTCTTTTGGTAGAGTGCGGCTCAGAACTTCAACTTTCACGAGATGCAATTTTGGATGCTTTAAGTGCAAAGAATTCTAAGGAATTGGAATCAACTGCAACTACATCACAAGTATCAGTTTTAGAGACTTCTTTATTAATTGAATGTTGTGGTCAAGTCACGTTTCAGAAACTAAAAAATCCCTGAGGTCTCTTCTAATTCGAATGGGAATGAATTGCCAAGCTGTCGATATATTTGTTACATTTTTATATATAAAATATTAGAACAGTAAGTTTCGTATAAAAATAGATGAGCACTTAAAGTCTTATAGTTCTGGTTGTTGAATTATTTTTGATAATACCAATTATATGTGAAGAGCCGAGTGTATTGTGGTGTAGTAGTTGGCACTTCTAGTCCCCTTCCCGGCTACTGGACCACTTCATTTCTTTGGGTACTACGGACCCCCTCTGCCATCCATTGCAGCAGAGTCGTTTCAGGAGGGGGGGCTAAGCACAATTTTTACTTCAGAACACTGTCAGATGATCATATTTTTTTACTGATAAAAATGATTATGTTATGAGGAAGGTTACATCTTTCATTGTTGAATAAATGATAATGTGATATTAATATATTAGTACAATTTTGAGCCTACTTTCACGGTTTACAGAATACGACTGTCAAGTCATGTGGTGGTTTCTTGCTCCCTGGTGATAATTATCCAAATTGACTAGCCTTCAATTATGACGGTTCTTCTGTAATTTTTATAGTTCCTCAAGTGAAAGGGCATAACTTGAAGACAATGATGTGCTTTGTCTATTCTTCAAGCGCAGATAACATAACATCAGATGGACTCAAAAATGTGTTGGTGATAAATCACACGAAGAGCACATTCAGCTCTATAAGAGAGAGGCGTTCGCCACCTTTGAAAATGAGGAGTGGCAGAGAGTAGTATCAAATATGGAACCTGGTGACAAAGTCGAGATTGTTGTTGTTTTTGGGAACAGTTTTATTGTGATGAAGACAGCAGTTTATCTCATATATGATGAACCAGTTGGCGAAAATTTGGGGCAATGTCATACTCCAGATAAGAATGTTCTTGTTGGTGATGGTGATGAAAATGAATGTCCTGCCAAGAGGATCACTCTTCAAGTAGAGCTGGCAGAAGATTTCAATCAAAAACAGAAAATAAGAAAACTTGACTGA
Protein sequence

>MS.gene052790.t1

MPNLEKLILKDCPRLSEVSHTTGHLHKEDLEQMESLTTLIADDTAITEVPFSLTRWTQKCVGDKSHEEHIQLYKREAFATFENEEWQRVVSNMEPGDKVEIVVVFGNSFIVMKTAVYLIYDEPVGENLGQCHTPDKNVLVGDGDENECPAKRITLQVELAEDFNQKQKIRKLD