AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07876


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07876.t1 MTR_8g019660 93.201 353 13 3 1 347 58 405 0.0 561
MS.gene07876.t1 MTR_3g104880 42.960 277 109 10 73 341 136 371 1.91e-39 143
MS.gene07876.t1 MTR_4g125620 37.644 348 139 14 10 347 65 344 4.28e-28 112
MS.gene07876.t1 MTR_1g015275 39.373 287 110 8 67 333 123 365 4.26e-22 96.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07876.t1 AT3G13980 51.636 275 88 10 78 347 123 357 2.70e-64 208
MS.gene07876.t1 AT1G54200 48.175 274 112 9 76 342 115 365 1.82e-54 182
MS.gene07876.t1 AT3G42800 37.719 228 111 13 125 347 140 341 2.62e-25 104
MS.gene07876.t1 AT5G12050 39.726 219 98 9 134 333 153 356 9.22e-12 65.9

Find 43 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATAATTTATCAGTAAT+TGG 0.114503 8.3:-70967905 MS.gene07876:CDS
TTCAATCCCAATAGAGTTAT+TGG 0.119420 8.3:-70967836 MS.gene07876:CDS
ATGTGATGCAAGAGAGTTTA+AGG 0.174902 8.3:-70968060 MS.gene07876:CDS
ACCATTTCCAATAACTCTAT+TGG 0.241032 8.3:+70967829 None:intergenic
TCAGAAACACTAGTTCTAAT+TGG 0.278598 8.3:+70968595 None:intergenic
AACATTGATTCATTTCAATA+TGG 0.291124 8.3:-70968565 MS.gene07876:CDS
AACTACTACAAATTCTGTTT+TGG 0.296788 8.3:-70968314 MS.gene07876:CDS
TCTGATTCAGAATCATTCTA+TGG 0.300272 8.3:-70968634 MS.gene07876:CDS
ACACTAGTTCTAATTGGTTT+TGG 0.307037 8.3:+70968601 None:intergenic
CCCAATAGAGTTATTGGAAA+TGG 0.315105 8.3:-70967830 MS.gene07876:CDS
CCATTTCCAATAACTCTATT+GGG 0.327955 8.3:+70967830 None:intergenic
AAGCAAGAAAGGTGGAGAAT+TGG 0.331562 8.3:-70968792 MS.gene07876:CDS
GAACAGAATGGTTATGGTTA+AGG 0.337246 8.3:+70968530 None:intergenic
AAATGAGTAGTTTCATAAAC+TGG 0.342032 8.3:+70967857 None:intergenic
TGGATAATTTATCAGTAATT+GGG 0.347307 8.3:-70967904 MS.gene07876:CDS
GAGTTTGAACAGAATGGTTA+TGG 0.352789 8.3:+70968524 None:intergenic
TATTGAAATGAATCAATGTT+TGG 0.355365 8.3:+70968568 None:intergenic
TCATCAACAATAACACTAAC+AGG 0.374723 8.3:+70968178 None:intergenic
ATACAAAATTCTCAAGGCTT+TGG 0.374949 8.3:+70968462 None:intergenic
AGAGTAGTAGTGAAGAATTA+AGG 0.394514 8.3:-70968087 MS.gene07876:CDS
GAATTTGCATGAAGGTGAAA+AGG 0.410666 8.3:-70968131 MS.gene07876:CDS
TAGTTCTGATTTGTTTGAAT+TGG 0.413467 8.3:-70967924 MS.gene07876:CDS
TCTTGTTTCAAATTCTGTTA+AGG 0.414977 8.3:+70968741 None:intergenic
TATCAGTAATTGGGATTGAA+AGG 0.444002 8.3:-70967895 MS.gene07876:CDS
TTAGCACCTGATCTTGATGA+AGG 0.453605 8.3:+70968220 None:intergenic
TTATTGTTGATGAAGATTGT+AGG 0.478035 8.3:-70968168 MS.gene07876:CDS
AAAACTCCTTCATCAAGATC+AGG 0.481984 8.3:-70968226 MS.gene07876:CDS
AAGGGTTCTTCAATATATGA+TGG 0.489828 8.3:-70968112 MS.gene07876:CDS
TCTTCAGAATCAAGTTCAGC+TGG 0.492878 8.3:-70968670 MS.gene07876:CDS
AATTTGCATGAAGGTGAAAA+GGG 0.494062 8.3:-70968130 MS.gene07876:CDS
TGCAAATTCTTGTGTCCACA+TGG 0.508348 8.3:+70968145 None:intergenic
AAACTTCAGAAAAGCAAGAA+AGG 0.514735 8.3:-70968803 MS.gene07876:CDS
TGTGATGCAAGAGAGTTTAA+GGG 0.547284 8.3:-70968059 MS.gene07876:CDS
GATGAAGATGAAGATGATGA+TGG 0.547702 8.3:-70967959 MS.gene07876:CDS
AGAAGATGAACTTGAATGCA+AGG 0.550199 8.3:+70968690 None:intergenic
TGGAATGAGAAAGTTGTGAA+TGG 0.552681 8.3:-70968772 MS.gene07876:CDS
ATCACCATACAAAATTCTCA+AGG 0.600006 8.3:+70968456 None:intergenic
TCAGAATCAAGTTCAGCTGG+TGG 0.600576 8.3:-70968667 MS.gene07876:CDS
CTTCAGAAAAGCAAGAAAGG+TGG 0.635352 8.3:-70968800 MS.gene07876:CDS
ACAAAGCAACCTATTTCACC+TGG 0.653006 8.3:-70968424 MS.gene07876:CDS
GGACACAAGAATTTGCATGA+AGG 0.715993 8.3:-70968139 MS.gene07876:CDS
GATGAAGATTGTAGGCCATG+TGG 0.719882 8.3:-70968160 MS.gene07876:CDS
CAGGTGCTAAAAGATCAGTG+AGG 0.837686 8.3:-70968207 MS.gene07876:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGAAATGAATCAATGTT+TGG + chr8.3:70968090-70968109 None:intergenic 20.0%
!! TGGATAATTTATCAGTAATT+GGG - chr8.3:70968751-70968770 MS.gene07876:CDS 20.0%
!! TTGGATAATTTATCAGTAAT+TGG - chr8.3:70968750-70968769 MS.gene07876:CDS 20.0%
!!! AACATTGATTCATTTCAATA+TGG - chr8.3:70968090-70968109 MS.gene07876:CDS 20.0%
!!! TGATGGTATTTTTGATTTTT+TGG + chr8.3:70968307-70968326 None:intergenic 20.0%
! AAATGAGTAGTTTCATAAAC+TGG + chr8.3:70968801-70968820 None:intergenic 25.0%
! CAAAAACCAAAAAATGAGAA+TGG - chr8.3:70968153-70968172 MS.gene07876:CDS 25.0%
!! AACTACTACAAATTCTGTTT+TGG - chr8.3:70968341-70968360 MS.gene07876:CDS 25.0%
!! TCTTGTTTCAAATTCTGTTA+AGG + chr8.3:70967917-70967936 None:intergenic 25.0%
!! TTATTGTTGATGAAGATTGT+AGG - chr8.3:70968487-70968506 MS.gene07876:CDS 25.0%
!!! AATTCACTTTTCAATTCTAG+TGG - chr8.3:70968276-70968295 MS.gene07876:CDS 25.0%
!!! TAGTTCTGATTTGTTTGAAT+TGG - chr8.3:70968731-70968750 MS.gene07876:CDS 25.0%
AAACTTCAGAAAAGCAAGAA+AGG - chr8.3:70967852-70967871 MS.gene07876:CDS 30.0%
AAGGGTTCTTCAATATATGA+TGG - chr8.3:70968543-70968562 MS.gene07876:CDS 30.0%
AATTTGCATGAAGGTGAAAA+GGG - chr8.3:70968525-70968544 MS.gene07876:CDS 30.0%
ACCATTTCCAATAACTCTAT+TGG + chr8.3:70968829-70968848 None:intergenic 30.0%
AGAGTAGTAGTGAAGAATTA+AGG - chr8.3:70968568-70968587 MS.gene07876:CDS 30.0%
ATACAAAATTCTCAAGGCTT+TGG + chr8.3:70968196-70968215 None:intergenic 30.0%
ATCACCATACAAAATTCTCA+AGG + chr8.3:70968202-70968221 None:intergenic 30.0%
CCAAAAAATGAGAATGGTTT+AGG - chr8.3:70968159-70968178 MS.gene07876:CDS 30.0%
CCATTTCCAATAACTCTATT+GGG + chr8.3:70968828-70968847 None:intergenic 30.0%
TATCAGTAATTGGGATTGAA+AGG - chr8.3:70968760-70968779 MS.gene07876:CDS 30.0%
TCATCAACAATAACACTAAC+AGG + chr8.3:70968480-70968499 None:intergenic 30.0%
TCTGATTCAGAATCATTCTA+TGG - chr8.3:70968021-70968040 MS.gene07876:CDS 30.0%
TTCAATCCCAATAGAGTTAT+TGG - chr8.3:70968819-70968838 MS.gene07876:CDS 30.0%
! AAAGCCTTGAGAATTTTGTA+TGG - chr8.3:70968195-70968214 MS.gene07876:CDS 30.0%
!! TCAGAAACACTAGTTCTAAT+TGG + chr8.3:70968063-70968082 None:intergenic 30.0%
!!! ACACTAGTTCTAATTGGTTT+TGG + chr8.3:70968057-70968076 None:intergenic 30.0%
!!! CCTAAACCATTCTCATTTTT+TGG + chr8.3:70968162-70968181 None:intergenic 30.0%
!!! GTTTTTGAGTTTGAACAGAA+TGG + chr8.3:70968140-70968159 None:intergenic 30.0%
!!! TTTCTTGCTTTTCTGAAGTT+TGG + chr8.3:70967853-70967872 None:intergenic 30.0%
AAAACTCCTTCATCAAGATC+AGG - chr8.3:70968429-70968448 MS.gene07876:CDS 35.0%
AGAAGATGAACTTGAATGCA+AGG + chr8.3:70967968-70967987 None:intergenic 35.0%
ATGTGATGCAAGAGAGTTTA+AGG - chr8.3:70968595-70968614 MS.gene07876:CDS 35.0%
CCCAATAGAGTTATTGGAAA+TGG - chr8.3:70968825-70968844 MS.gene07876:CDS 35.0%
GAACAGAATGGTTATGGTTA+AGG + chr8.3:70968128-70968147 None:intergenic 35.0%
GAATTTGCATGAAGGTGAAA+AGG - chr8.3:70968524-70968543 MS.gene07876:CDS 35.0%
GAGTTTGAACAGAATGGTTA+TGG + chr8.3:70968134-70968153 None:intergenic 35.0%
GATGAAGATGAAGATGATGA+TGG - chr8.3:70968696-70968715 MS.gene07876:CDS 35.0%
TGGAATGAGAAAGTTGTGAA+TGG - chr8.3:70967883-70967902 MS.gene07876:CDS 35.0%
TGTGATGCAAGAGAGTTTAA+GGG - chr8.3:70968596-70968615 MS.gene07876:CDS 35.0%
! ATTTTTGCACCAGGTGAAAT+AGG + chr8.3:70968243-70968262 None:intergenic 35.0%
!! CATCAGTGTCTTCTTTTTCA+AGG - chr8.3:70968394-70968413 MS.gene07876:CDS 35.0%
!! GTTTTTGTAGCTGTTGATGA+TGG + chr8.3:70968324-70968343 None:intergenic 35.0%
AAGCAAGAAAGGTGGAGAAT+TGG - chr8.3:70967863-70967882 MS.gene07876:CDS 40.0%
ACAAAGCAACCTATTTCACC+TGG - chr8.3:70968231-70968250 MS.gene07876:CDS 40.0%
CTTCAGAAAAGCAAGAAAGG+TGG - chr8.3:70967855-70967874 MS.gene07876:CDS 40.0%
GGACACAAGAATTTGCATGA+AGG - chr8.3:70968516-70968535 MS.gene07876:CDS 40.0%
TCTTCAGAATCAAGTTCAGC+TGG - chr8.3:70967985-70968004 MS.gene07876:CDS 40.0%
TGCAAATTCTTGTGTCCACA+TGG + chr8.3:70968513-70968532 None:intergenic 40.0%
TTAGCACCTGATCTTGATGA+AGG + chr8.3:70968438-70968457 None:intergenic 40.0%
! AAGCTAGCAATTTTTGCACC+AGG + chr8.3:70968252-70968271 None:intergenic 40.0%
CAGGTGCTAAAAGATCAGTG+AGG - chr8.3:70968448-70968467 MS.gene07876:CDS 45.0%
GATGAAGATTGTAGGCCATG+TGG - chr8.3:70968495-70968514 MS.gene07876:CDS 45.0%
TCAGAATCAAGTTCAGCTGG+TGG - chr8.3:70967988-70968007 MS.gene07876:CDS 45.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 70967817 70968860 70967817 ID=MS.gene07876
chr8.3 mRNA 70967817 70968860 70967817 ID=MS.gene07876.t1;Parent=MS.gene07876
chr8.3 exon 70967817 70968860 70967817 ID=MS.gene07876.t1.exon1;Parent=MS.gene07876.t1
chr8.3 CDS 70967817 70968860 70967817 ID=cds.MS.gene07876.t1;Parent=MS.gene07876.t1
Gene Sequence

>MS.gene07876

ATGAGGAAGATGAAAAGTGTTGAAGCTGAAAAACCAAACTTCAGAAAAGCAAGAAAGGTGGAGAATTGGAATGAGAAAGTTGTGAATGGAAGAAACTCCTTAACAGAATTTGAAACAAGAACAACAAGAAGCAATTCAAACACACTCTCCTTGCATTCAAGTTCATCTTCTTCAGAATCAAGTTCAGCTGGTGGTTTTTCATCATCTGATTCAGAATCATTCTATGGTTTACAAAAACCAAAACCAATTAGAACTAGTGTTTCTGAAAAACCAAACATTGATTCATTTCAATATGGTTATAGTAGAAACCTTAACCATAACCATTCTGTTCAAACTCAAAAACCAAAAAATGAGAATGGTTTAGGTAAAACAAAGTCCAAAGCCTTGAGAATTTTGTATGGTGATTTGAAGAAAACAAAGCAACCTATTTCACCTGGTGCAAAAATTGCTAGCTTTTTGAATTCACTTTTCAATTCTAGTGGAAATACCAAAAAATCAAAAATACCATCATCAACAGCTACAAAAACTACTACAAATTCTGTTTTGGAAGCAAAATCAGCACAAGCTTCAACATGTTCATCAGTGTCTTCTTTTTCAAGGTCTTGTTTGAGTAAAACTCCTTCATCAAGATCAGGTGCTAAAAGATCAGTGAGGTTTTGTCCTGTTAGTGTTATTGTTGATGAAGATTGTAGGCCATGTGGACACAAGAATTTGCATGAAGGTGAAAAGGGTTCTTCAATATATGATGGAAAGAGTAGTAGTGAAGAATTAAGGTTGCATGTGATGCAAGAGAGTTTAAGGGTTGAAGAGTTAGCTAGAGACTTGTTGAAGAATTATCAGAAAAAAAATGAAGTTGATTTTAATATGCAATTTGAAGATGATGAAGATGAAGATGATGATGGAGCTAGTTGTGCTAGTTCTGATTTGTTTGAATTGGATAATTTATCAGTAATTGGGATTGAAAGGTATAGAGAAGAATTACCAGTTTATGAAACTACTCATTTCAATCCCAATAGAGTTATTGGAAATGGTTTCATTATGTAA

Protein sequence

>MS.gene07876.t1

MRKMKSVEAEKPNFRKARKVENWNEKVVNGRNSLTEFETRTTRSNSNTLSLHSSSSSSESSSAGGFSSSDSESFYGLQKPKPIRTSVSEKPNIDSFQYGYSRNLNHNHSVQTQKPKNENGLGKTKSKALRILYGDLKKTKQPISPGAKIASFLNSLFNSSGNTKKSKIPSSTATKTTTNSVLEAKSAQASTCSSVSSFSRSCLSKTPSSRSGAKRSVRFCPVSVIVDEDCRPCGHKNLHEGEKGSSIYDGKSSSEELRLHVMQESLRVEELARDLLKNYQKKNEVDFNMQFEDDEDEDDDGASCASSDLFELDNLSVIGIERYREELPVYETTHFNPNRVIGNGFIM